SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Dandan Fang,Huizhu Ren,Yitian Huang,Hong Dai,Defu Huang,Yanyu Lin
Herein, an ultrasensitive electrochemiluminescence (ECL) immunosensor based on a high performance probe of Nb 2 CTx MXene immobilized ZnO quantum dots (QDs) was constructed for Thyroglobulin (Tg) detection. ZnO QDs with good biocompatibility and catalytic property was for the first time utilized as coreaction accelerator in Ru(bpy) 3 2+ /S 2 O 8 2− system and enhanced ECL signal. Specifically, ZnO QDs can facilitate S 2 O 8 2− reduction to generate more SO 4 − , and then reacted with cathodic radical of Ru(bpy) 3 2+ , realizing 2-fold enhancement in ECL intensity. Additionally, Nb 2 CTx MXene possesses excellent properties and was used as prominent supporter, improving the loading capacity of Ru(bpy) 3 2+ and ZnO QDs, and accelerating the electron transfer, resulting in ECL signal enhancement. Furthermore, Nb 2 CTx MXene acted as temperature controller and increased the electrode surface temperature by converting laser energy into heat, further amplified ECL signal. Because of the high sensitivity, good specificity and favorable stability, the proposed immunosensor realized sensitive detection of Thyroglobulin and had a great application prospect in bioanalysis and clinical diagnosis.
研究领域:
分析化学
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生物医学工程
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临床诊断
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Qiao-Fen Pan,Hai-Feng Jiao,Hua Liu,Jin-Jie You,Ai-Li Sun,Ze-Ming Zhang,Xi-Zhi Shi
A novel molecularly imprinted electrochemiluminescence (MIECL) sensor based on the luminescence of molecularly imprinted polymer-perovskite (MIP-CsPbBr 3 ) layer and Ru(bpy) 3 2+ was fabricated for simazine detection. MIP-CsPbBr 3 layers were immobilized onto the surface of glassy carbon electrode as the capture and signal amplification probe, and Ru(bpy) 3 2+ and co-reactant tripropylamine exhibited stronger electrochemiluminescence (ECL) emission. Under optimal conditions, the ECL signal of the MIECL sensor was linearly quenched, with the logarithm of simazine concentration ranging from 0.1 μg/L to 500.0 μg/L, correlation coefficient of 0.9947, and limit of detection of 0.06 μg/L. The practicality of the developed MIECL sensor method for simazine determination in aquatic samples was validated. Excellent recoveries of 86.5 %–103.9 % with relative standard deviation below 1.6 % were obtained for fish and shrimp samples at three different spiked concentrations. The MIECL sensor exhibited excellent selectivity , sensitivity, reproducibility, accuracy, and precision for simazine determination in actual aquatic samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Li Jingxian,Shan Xueling,Jiang Ding,Wang Wenchang,Chen Zhidong
Ultrasensitive and selective determination of bisphenol A (BPA) was achieved by a solid-state electrochemiluminescence (ECL) aptasensor. The Ti-based MOFs (MIL-125, MIL stands for Materials of Institute Lavoisier) have porous structure and large specific surface area. They were used as a carrier for Ru(bpy) 3 2+ by a hydrothermal synthesis method. The Ru(bpy) 3 2+ encapsulated MIL-125 (Ru(bpy) 3 2+ @MIL-125) was dropped on a glass carbon electrode (GCE) and used as working electrode. After that, the thiol-based aptamer of BPA was used to modify the working electrode by Ti–S bond. As a result of the specific binding affinity between aptamer and BPA, the ECL signal was significantly quenched. Thus, a selective electrochemiluminescence (ECL) aptasensor for BPA was developed. The ECL aptasensor based on Ru(bpy) 3 2+ @MIL-125 has a good ECL response toward detecting BPA. Under optimal conditions, the aptasensor can detect BPA in a wide linear range from 1.0 × 10 −12 to 1.0 × 10 −6 M with an excellent determination limit of 6.1 × 10 −13 M (S/ N = 3). The ECL aptasensor can selectively detect BPA in a mixture containing BPA interference compounds.
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Yang Luoxing,Zhang Tongtong,Zhou Huaxu,Yan Fei,Liu Yan
Vertically ordered mesoporous silica film (VMSF) with uniform mesoporous channels perpendicular to electrode substrate has a wide range of applications in direct electroanalysis of complex samples. However, the detection of nucleic acid bases is difficult to realize at the commonly used VMSF-modified indium tin oxide (VMSF/ITO) electrode due to the high overpotentials of underlying ITO for many small organic molecules. In this work, we demonstrated an electrochemical method for the sensitive detection of guanine (G) by integration of VMSF/ITO and tris(2,2′-bipyridine) ruthenium (II) [Ru(bpy)32+] redox mediator. Ru(bpy)32+ electrostatically accumulated by VMSF is able to act as an electron shuttle between G and underlying ITO surface, showing electrocatalytic oxidation of G and enabling the quantitative determination of G with a limit of detection (LOD) of 0.058 μM and a limit of quantitation (LOQ) of 0.2 μM. Electrochemical detection performance for G could be regulated by changing the pH of the supporting electrolyte and the content of Ru(bpy)32+, achieving a wide dynamic linear range from 0.2 to 10 μM (R2 = 0.999), 2 to 100 μM (R2 = 0.999), and 10 to 500 μM (R2 = 0.998). Furthermore, owing to the good anti-fouling and anti-interference ability of VMSF, this simply sensing strategy can be applied to the direct and rapid detection of G in beer samples, and the detection of ganciclovir (G analog) content in ganciclovir eye drops.
研究领域:
分析化学
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纳米技术
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电化学
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生物传感器
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药物分析
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2014
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IF 4.517
Limin Zhou,Jianshe Huang,Lu Yang,Libo Li,Tianyan You
A sensitive electrochemiluminescence (ECL) sensor for melamine analysis was fabricated based on Ru(bpy) 3 2+ -doped silica (Ru(bpy) 3 2+ @SiO 2 ) nanoparticles and graphene composite. Spherical Ru(bpy) 3 2+ @SiO 2 nanoparticles with uniform size about 55 nm were prepared by the reverse microemulsion method. Since per Ru(bpy) 3 2+ @SiO 2 nanoparticle encapsulated a great deal of Ru(bpy) 3 2+ , the ECL intensity has been greatly enhanced, which resulted in high sensitivity. Due to its extraordinary electric conductivity, graphene improved the conductivity and accelerated the electron transfer rate. In addition, graphene could work as electronic channel improving the efficient luminophor amount participating in the ECL reaction, which further enhanced the ECL signal. This proposed sensor was used to melamine analysis and the ECL intensity was proportional to logarithmic melamine concentration range from 1 × 10 −13 M to 1 × 10 −8 M with the detect limit as low as 1 × 10 −13 M. In application to detect melamine in milk, satisfactory recoveries could be obtained, which indicated this sensor having potential application in melamine analysis in real samples.
研究领域:
分析化学
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食品安全检测
·
材料科学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
·
IF 4.097
Amin Zhang,Chongchong Miao,Hongyuan Shi,Hongkun Xiang,Chusen Huang,Nengqin Jia
Recent years have witnessed the great development of electrochemiluminescence (ECL) as a platform for the analytical processes in many fields, which includes pharmaceutical analysis, immunoassay, DNA analysis as well as clinical analysis. Herein, a novel electrochemiluminescence (ECL) sensor was fabricated for ultrasensitive detection of atropine based on Ru(bpy) 3 2+ /carbon nanospheres/Nafion composite film. The carbon nanospheres (CNPs) in composite film were obtained through a hydrothermal method. Then, electrochemical and ECL behaviors of the proposed sensor were investigated. Under the optimal conditions, the constructed atropine sensor showed a wide linear range from 0.5 to 250 μM with a detection limit of 0.17 μM (at signal-to-noise ratio of 3). Moreover, the results indicated that the CNPs-based ECL sensor exhibited excellent sensitivity and favorable stability, which might be ascribed to the high surface area and the good electrical conductivity of CNPs. Furthermore, the ECL sensor was applied for the determination of atropine in atropine sulfate injection samples and displayed a desirable result. The proposed ECL sensor could be easily obtained and be promising used for drug assay.
研究领域:
分析化学
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药学
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电化学
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纳米技术
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产品关联
DOI
高影响因子
RSC Advances
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2015
·Vol. 5
·期 114
·94338-94343
·
IF 3.84
Qinghong Wang,Xia Lin,Dongmei Guo,Juanjuan Xu,Chunzhi Xuan,Cui Chen,Yingzi Fu
A sensitive, stable and stereoselective electrochemiluminescence (ECL) sensor has been designed to enantioselectively discriminate proline enantiomers by immobilizing Ru(bpy)32+–gold nanoparticles (Ru–AuNPs) and β-cyclodextrin–reduced graphene oxide (β-CD–rGO) on glassy carbon electrode. More Ru(bpy)32+ could be immobilized on the surface of electrode stably via preparing Ru–AuNPs and better stereoselectivity could be introduced to the sensor via the synthesis of β-CD–rGO. When the developed sensor interacted with proline enantiomers, obvious difference of ECL intensities towards L- and D-proline was observed, and a larger intensity was obtained from D-proline. As a result, ECL technique might act as a promising method to chiral recognition of amino acids enantiomers or chiral drugs.
研究领域:
分析化学
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生物传感
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材料科学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-11
·
IF 5.3
Lu Juan,Li Chao,Guo Yanjia,Feng Yongjia,Song Yujia,Li Ruidan,Tian Li,Wang Jing
Loofah sponge-like carbon nanofibers (LF-Co,N/CNFs) were utilized as a carrier for Ru(bpy) 3 2+ , and then combined with CdS to create a novel solid-state electrochemiluminescence sensor capable of detecting trace amounts of fenpropathrin. LF-Co,N/CNFs, obtained through the high-temperature pyrolysis of ZIF-67 coaxial electrospinning fibers, were characterized by a loofah-like morphology and exhibited a significant specific surface area and porosity. Apart from serving as a carrier, LF-Co,N/CNFs also functioned as a luminescence accelerator, enhancing the system's luminescence efficiency by facilitating electron transmission and reducing the transmission distance. The inclusion of CdS in the luminescence reaction, in conjunction with Ru(bpy) 3 2+ , further boosted the sensor's luminescence signal. The resulting sensor demonstrated a satisfactory signal, with fenpropathrin causing significant quenching of the ECL signal. Under optimized conditions, a linear relationship between the signal quench value and fenpropathrin concentration in the range 1 × 10 –12 to 1 × 10 –6 M was observed, with a detection limit of 3.3 × 10 –13 M (S/N = 3). This developed sensor is characterized by its simplicity, sensitivity, and successful application in detecting fenpropathrin in real samples. The study not only presents a straightforward detection platform for fenpropathrin but also introduces new avenues for the rapid determination of various food contaminants, thereby expanding the utility of carbon fibers in electrochemiluminescence sensors. Graphical Abstract
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2015
·
IF 4.772
Wenjing Lian,Xue Yu,Lei Wang,Hongyun Liu
A series of biomacromolecular logic gates and functional devices with cyclic voltammetric (CV) and electrochemiluminescence (ECL) responses as output signals were established on the basis of molecularly imprinted polymer (MIP) film electrodes. The MIP films were electropolymerized on the surface of Au electrodes with o-phenylenediamine (OPD) as the monomer and chloramphenicol (CP) as the template molecule. The simultaneous CV and ECL signals of Ru(bpy)32+ were significantly enhanced by the addition of natural DNA in solution at the MIP film electrodes after CP removal. The CV and ECL responses of the Ru(bpy)32+–DNA system at the MIP film electrodes were greatly influenced by CP removal and rebinding. Moreover, the addition of ferrocene methanol (FcMeOH) to the solution led to substantial quenching of the ECL signal and produced a new CV peak pair. On the basis of these results, 3-input/3-output and 3-input/5-output biomacromolecular logic gate systems were established with DNA, CP, and FcMeOH as inputs and the ECL responses at different levels or CV responses at different potentials as outputs. After an elaborate reconfiguration of inputs and outputs, the functional non-Boolean logic devices such as a 2-to-1 encoder, a 1-to-2 decoder, and a 1-to-2 demultiplexer were also constructed on the same platform.
研究领域:
电分析化学
·
生物传感器
·
分子电子学
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生物纳米技术
·
化学逻辑器件
✓ 已验证
DOI
高影响因子
Research
·
2023
·
IF 11
Shijun Wang,Shu Zhu,Ziqi Kang,Xiangxiu Wang,Zixin Deng,Kun Hu,Jianjun Hu,Xiancheng Liu,Guixue Wang,Guangchao Zang,Yuchan Zhang
Multiple signal strategies remarkably improve the accuracy and efficiency of electrochemiluminescence (ECL) immunoassays, but the lack of potential-resolved luminophore pairs and chemical cross talk hinders their development. In this study, we synthesized a series of gold nanoparticles (AuNPs)/reduced graphene oxide (Au/rGO) composites as adjustable oxygen reduction reaction and oxygen evolution reaction catalysts to promote and modulate tris(2,2′-bipyridine) ruthenium(II) (Ru(bpy)32+)’s multisignal luminescence. With the increase in the diameter of AuNPs (3 to 30 nm), their ability to promote Ru(bpy)32+’s anodic ECL was first impaired and then strengthened, and cathodic ECL was first enhanced and then weakened. Au/rGOs with medium-small and medium-large AuNP diameters remarkably increased Ru(bpy)32+’s cathodic and anodic luminescence, respectively. Notably, the stimulation effects of Au/rGOs were superior to those of most existing Ru(bpy)32+ co-reactants. Moreover, we proposed a novel ratiometric immunosensor construction strategy using Ru(bpy)32+’s luminescence promoter rather than luminophores as tags of antibodies to achieve signal resolution. This method avoids signal cross talk between luminophores and their respective co-reactants, which achieved a good linear range of 10−7 to 10−1 ng/ml and a limit of detection of 0.33 fg/ml for detecting carcinoembryonic antigen. This study addresses the previous scarcity of the macromolecular co-reactants of Ru(bpy)32+, broadening its application in biomaterial detection. Furthermore, the systematic clarification of the detailed mechanisms for converting the potential-resolved luminescence of Ru(bpy)32+ could facilitate an in-depth understanding of the ECL process and should inspire new designs of Ru(bpy)32+ luminescence enhancers or applications of Au/rGOs to other luminophores. This work removes some impediments to the development of multisignal ECL biodetection systems and provides vitality into their widespread applications.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Fan Yuan,Kai Hao,Shu Sheng,Tadesse Haile Fereja,Xiangui Ma,Feng Liu,Muhammad Nadeem Zafar,Baohua Lou,Huayu Tian,Guobao Xu
A highly efficient co-reactant of Ru(bpy) 3 2+ electrochemiluminescence (ECL), 2-(dibutylamino)ethyl acrylate(DBAEA), was successfully synthesized by a straightforward one-step reaction between dibutylaminoethanol and acrylyl chloride. On account of the interaction between the olefin group of DBAEA and Pt electrode, the ECL intensity utilizing DBAEA as the co-reactant was increased dramatically, nearly as 10 times and 17 times as conventional co-reactants dibutyaminoethanol and tripropylamine . Since cysteine can remove the acrylate group of DBAEA specifically through conjugated addition/cyclization reaction, the ECL intensity of Ru(bpy) 3 2+ /DBAEA was declined selectively. Therefore, cysteine was detected through this novel ECL system with high selectivity towards other amino acids . The linear range was between 10 μM and 200 μM with a limit of detection of 1.15 μM. The cysteine in Dulbecco’s Modified Eagle Medium (DMEM) was detected and satisfying recovery was obtained.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Chen Cui,Xinyao Lin,Jie Lv,Hang Guo,Lu Shen,Guoqiang Xiang,Wenjie Zhao,Dechen Jiang
Designing innovative electrochemiluminescence (ECL) immunosensors is critical for the detection of biomarkers with a low concentration and the precise evaluation of clinical diseases. Herein, a Cu 3 (hexahydroxytriphenylene) 2 (Cu 3 (HHTP) 2 ) nanoflake-based sandwich-type ECL immunosensor was constructed for C-Reactive Protein (CRP) detection. The Cu 3 (HHTP) 2 nanoflake , an electronically conductive metal–organic framework (MOF), has a periodically arranged porous structure with a cavity size of 2 nm, which not only accommodates a large amount of Ru(bpy) 3 2+ but also confines the spatial diffusion of active species. Therefore, the Ru(bpy) 3 2+ -loaded Cu 3 (HHTP) 2 nanocomplex (Ru@CuMOF) as an ECL emitter exhibits an enhanced ECL efficiency. The ECL resonance energy transfer (ECL-RET) was accomplished by combining Ru@CuMOF used as a donor with gold nanoparticles-functionalized graphene oxide nanosheets (GO-Au) utilized as an acceptor. This should be ascribed to the fact that the ECL emission spectrum of Ru@CuMOF shows the strongest signal intensity at 615 nm, overlapping with the absorption spectrum of GO-Au at 580–680 nm. Targeted detection of CRP in human serum samples was achieved by the sandwich-type immunosensor based on the ECL-RET mechanism with a 0.26 pg mL −1 detection limit. The electro-activated hybrids of Cu 3 (HHTP) 2 and ECL emitters provide a new sensing strategy for the high-sensitivity detection of disease markers.
研究领域:
生物医学工程
·
分析化学
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）