Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Zang Yuan-Yuan,Yang Sha,Xu Yong-Qiang,Chen Zhi-Gang,Wu Tao
In this study, a strain with rutin degrading enzyme (RDE) activity was screened from moldy tartary buckwheat and subsequently identified as Fusarium spp. The structure and enzyme characteristics of CLEA-RDE formed by immobilization via cross-linking were then investigated. Further, the optimal catalysis conditions of CLEA-RDE in natural deep eutectic solvents (NADESs) serving as hydrolysis solvents were also investigated. The results of SEM and spectrum indicated that CLEA-RDE became more stable than free-RDE due to the cross-linking. Interestingly, CLEA-RDE showed a wider range of pH adaptation and higher tolerance to low temperatures (20 – 30°C) and hydrophobic environments. The results of orthogonal experiments revealed that the optimal condition for rutin hydrolysis was under pH 5.0 and 40oC with the degradation rate of 10.65 mg min−1 L−1. The preparation of CLEA-RDE without a carrier-based immobilization method reduces the loss of enzyme activity, improves the stability of the enzyme and can be applied to the investigation of immobilization of various enzymes, thus providing a referred idea for the improvement of catalysts in industrial production.
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高影响因子
CERAMICS INTERNATIONAL
·
2018
·
IF 3.057
Jiangjiang Zhang,Shiqiang Cui,Yaping Ding,Xinxin Yang,Kai Guo,Jingtai Zhao
The ordered mesoporous Co 3 O 4 nanospheres encapsulated with reduced graphene oxide (denoted as meso-Co 3 O 4 / RGO) were synthesized via electrostatic interaction and firstly for the electrochemical detection of rutin with good sensing effects. The resultant meso-Co 3 O 4 / RGO nanocatalyst not only possesses more active sites due to the high surface area deriving from the mesoporous structure, but also has benign conductibility due to the presence of RGO, both of which enhance the sensing properties for the electrochemical detection of rutin. The developed sensor displays low detection limit (0.03 μM) and large sensitivity (74.85 μA μM −1 cm −2 ). Besides, the rutin sensor possesses good selectivity, stability and reproducibility.
研究领域:
材料科学
·
分析化学
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高影响因子
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
·
2022
·Vol. 79
·期 2
·287-295
·
IF 5.08
Ye Fan,Lv Jiahua,Shen Xinyu,Zhang Jian,Zong Yingxin,Zhu Chan,Yang Yan,Jia Keke,Jiang Yucui,Tang Zongxiang
Rutin is a natural anti-inflammatory ingredient widely found in medicinal plants. Studies have shown that rutin inhibits mast cell degranulation and the release of inflammatory mediators. Mast cell P2X7 receptor mediates mast cell degranulation and serves as a therapeutic target for inflammatory pain. Herein, the aim of this study was to investigate whether the anti-inflammatory mechanism of rutin is related to the mast cell P2X7 receptor. Our results showed that rutin could inhibit [Ca 2+ ] i elevation induced by 5 mM ATP or 30 μM BZATP in a concentration-dependent manner in mouse peritoneal mast cells. Rutin also suppressed the inward current mediated by P2X7 receptor. In vivo, rutin could significantly inhibit the mechanical hypersensitivity induced by 100 mM ATP that is associated with P2X7 receptor in mast cells. Moreover, molecular docking revealed the high affinity between rutin and the P2X7 receptor crystal structure. Collectively, this study demonstrated that rutin attenuated inflammatory pain by inhibiting the activity of P2X7 receptor in mast cells.
研究领域:
药理学
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免疫学
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疼痛研究
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Tongtong Zhai,Yuan Xue,Yunhui Li,Jianwei Zhu,Jing Li,Erkang Wang
For hot electron-induced electrochemiluminescence (HECL), developing facile and controllable insulating layer on the electrode surface is key to the effective electron tunneling. Herein, a polystyrene modified glassy carbon electrode based the spin-coating was proposed for HECL . The constructed interface was characterized by atomic force microscopy (AFM). And HECL performance was investigated in details using the system of Ru(bpy) 3 2+ and S 2 O 8 2− . The as-prepared film shows the stable and reproducible HECL performance and the HECL mechanism was discussed using ECL spectra and hydrated electron quenchers. Finally, on the basis of the inhibition of SO 4 − , the present polystyrene film was applied to the detection of rutin in the range of 0.1–1.3 μM with a detection limit of 90 nM (S/N = 3).
研究领域:
分析化学
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电化学
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 3
·955
·
IF 4.6
Chaokang Chang,Meng Song,Mingxing Ma,Jihong Song,Fengyi Cao,Qi Qin
Rutin is a natural flavonoid that carries out a variety of biological activities, but its application in medicine and food is limited by its water solubility. One of the classical methods used to enhance drug solubility is encapsulation with cyclodextrins. In this paper, the encapsulation of different cyclodextrins with rutin was investigated using a combination of experimental and simulation methods. Three inclusions of rutin/beta-cyclodextrin (β-CD), rutin/2-hydroxypropyl beta-cyclodextrin (HP-β-CD) and rutin/2,6-dimethyl beta-cyclodextrin (DM-β-CD) were prepared by the freeze-drying method, and the inclusions were analyzed using Fourier infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), differential scanning calorimetry (DSC) and ultraviolet–visible spectroscopy (UV) to characterize and demonstrate the formation of the inclusion complexes. Phase solubility studies showed that rutin formed a 1:1 stoichiometric inclusion complex and significantly increased its solubility. β-CD, HP-β-CD, DM-β-CD, rutin and the three inclusion complexes were modeled by using MS2018 and AutoDock 4.0, and molecular dynamics simulations were performed to calculate the solubility parameters, binding energies, mean square displacement (MSD), hydrogen bonding and radial distribution functions (RDF) after the equilibration of the systems. The results of simulation and experiment showed that rutin/DM-β-CD had the best encapsulation effect among the three cyclodextrin inclusion complexes.Keywords:rutin;cyclodextrin;complex;molecular docking;molecular dynamics simulation
研究领域:
药学
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材料科学
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计算化学
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高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Zekun Li,Shuang Liang,Huijuan Sun,Cheng Bao,Yuan Li
Rutin, a flavonoid glycoside phytochemical compound, has a remarkable antiobesity effect. However, its therapeutic potential is hindered by its poor water solubility and low oral bioavailability. In this study, rutin was loaded into liposomes (LR) through the self-assembly of lecithin and cholesterol. It was discovered that liposomes improved the water solubility and cellular uptake of rutin in adipocytes. These rutin-loaded liposomes were then incorporated into a microneedle patch (MP) system formed by polyvinylpyrrolidone and poly(vinyl alcohol), and the MP-LR showed an increased release percentage in the adipose tissue microenvironment of pH 6.5 and achieved local delivery of rutin into adipocytes. Next, the therapeutic potentials of rutin, LR, and MP-LR were investigated in a high-fat diet (HFD)-induced obese mouse model. The MP-LR formulation decreased the weight of the HFD mice the most significantly. The antilipogenesis mechanisms of MP-LR are downregulating the lipid synthesis-related proteins (PPAR γ and C/EBP α) in adipocytes and promoting the expression of the beige adipogenesis-related proteins (UCP 1 and Cyt C). The MP systems further promote the local penetration of LR into the adipose tissue specifically, which again elevates their antiobesity effect. Overall, this study suggests that MP-delivered liposome-based formulation is a promising approach to enhance the antiobesity efficacy of antilipogenesis bioactive compounds.
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高影响因子
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
·
2020
·
IF 1.724
Kai Sheng,Qian Zhang,Lantao Li,YiLun Wang,Baoxian Ye
A new and sensitive electrochemical sensor for rutin determination was developed based on cetyltrimethylammonium chloride (CTAC) functionalized graphene (Gr) and palladium nanoparticles (Pd) (CTAC-Gr-PdNPs) composite. Rutin displayed remarkably increased electrochemical activity on the CTAC-Gr-PdNPs composite modified electrode due to the synergistic effect of the large surface area and electrocatalytic activity of both Gr and Pd nanoparticles, which offers the feasibility for highly sensitive determination of rutin via electrochemistry. Under the optimal experimental conditions, the oxidation peak current of rutin was proportional to its concentration in the range of 2.0 × 10−8–1.0 × 10−6 mol L−1, and the limit of detection (LOD) was 5 nM (S/N = 3). The developed method was successfully applied to determine rutin in pharmaceuticals with satisfactory recoveries, which shows that the fabricated sensor has potential in pharmaceutical analysis.
研究领域:
分析化学
·
药学
·
电化学
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-8
·
IF 5.479
Yalikun Nuerbiya,Mamat Xamxikamar,Li Yongtao,Hu Xun,Wang Ping,Hu Guangzhi
The authors describe a method for synthesis of a nanomaterial consisting of porous carbon encapsulated Mg-Al-Si alloy (denoted as Mg-Al-Si@PC) nanocluster. The nanocluster was synthesis by a solvothermal reaction, followed by high-temperature annealing. The nanoclusters were used as a novel immobilization platform for electrochemical sensing of rutin. The electrochemical behavior of rutin at a modified electrode was investigated by cyclic voltammetry and differential pulse voltammetry. The modified electrode demonstrates a high electrocatalytic activity toward rutin oxidation at a relatively low working potential (0.6 vs. Ag/AgCl). Under optimal conditions, the sensor has a linear response in the 1–10 μM rutin concentration range, and a 0.01 μM lower detection limit (at an S/N ratio of 3). It was successfully applied to the quantification of rutin in pharmaceutical tablets, and satisfactory results were obtained. Furthermore, the results correspond with those with the standard method and with the amounts indicated by the producer, respectively.
Schematic diagram of the Mg-Al-Si@PC nanocluster preparation process and electroanalysis mechanism.
研究领域:
材料科学
·
电化学
·
纳米技术
·
分析化学
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高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 7
·3489-3500
·
IF 4.1
Yihang Liu,Qingzhi Wang,Chen Liu,Haonan Yang,Li Jia,Litao Zhao,Fayong Gong,Chen Tan,Hongxun Tao,Wen-Sen He
BACKGROUND Oxidation is a major problem for oils and fats, which can be mitigated by antioxidants. Rutin has excellent antioxidant activity, but its poor lipid solubility greatly limits its practical application. In this study, an efficient enzymatic synthesis route of lipophilic rutin ester was established using oleic acid as an acyl donor, and the antioxidant potential of rutin oleate was evaluated for the first time by proton ( 1 H) nuclear magnetic resonance (NMR) spectroscopy. RESULTS The synthesized product was finally identified as rutin oleate by Fourier transform infrared, high-performance liquid chromatography–mass spectrometry, and 1 H, carbon-13, and DEPT-135 NMR analyses, and the acylation site was the 4‴-OH of the rhamnose group in the rutin molecule. The maximum conversion was over 93% after 48 h of reaction using Novozym 435 as catalyst under the best conditions among these tests. The conversion of rutin ester decreased with the increase of carbon chain length and the number of carbon–carbon double bonds of the fatty acid molecule. Most importantly, rutin oleate exhibited antioxidant capacity comparable to butylated hydroxytoluene and its counterparts (rutin and oleic acid) at low temperatures (60° C), but had a significant advantage at high temperatures (120° C). CONCLUSION The antioxidant activity of rutin was significantly enhanced by lipase-mediated esterification with oleic acid. Therefore, rutin oleate could be further developed as a novel antioxidant for use in oil‑ and fat-based foods. © 2023 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
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分析化学
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 6
·067526
·
IF 3.721
Yang Ma,Shaozhang Hou,Shujing Xue,Xuemei Wu,Haihao Yao,Keren Shi,Wei Ma,Lingxia Zuo,Huiqin Yao
The glassy carbon (GC) electrode surface modified with carboxylic multi-walled carbon nanotubes (c-MWCNTs) and poly(N-isopropylacrylamide) (PNIPAM) films were used to investigate multiple switching electrochemical behaviors of rutin, and a 4-input/3-output logic-gate system was constructed. The drug molecule rutin acted as electroactive probe, and displayed triple stimuli-responsive switchable cyclic voltammetry (CV) properties on c-MWCNTs/PNIPAM film electrodes. From pH 4.0 to pH 9.0 at 25 °C, the peak currents of rutin were decreasing with the pH value increasing due to the property of rutin itself and the electrostatic interaction between polyacrylamide (PAA) in the film and rutin molecule. When external temperature and Na2SO4 concentration increased, the CV response signals of rutin decreased because the hydrogen bonds between amide group of PNIPAM hydrogel polymer and water molecules were broken gradually, and a collapsed globule structure was formed. According to above results, multiple stimulus-responsive hydrogel thin film sensor was constructed. Then a 4-input/3-output logic-gate system including AND, NAND and INHIBIT gates was fabricated successfully. The construction of logic-gate system based on drug molecule rutin may provide a novel approach and model leading to design the new-style drug sensor, which might be applied to the intelligent medical diagnostics and drug release.
研究领域:
电化学
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材料科学
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生物传感器
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药物传递系统
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生物医学工程
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高影响因子
Separation Science Plus
·
2025
·Vol. 8
·期 11
·e70142
·
IF 1.6
Danyang Liu,Hao Zhou,Zhiwen Qi,Xianghui Kong,Chengzhang Wang
Ginkgo flavonol glycosides are widely used in the fields of food and medicine due to their free radical scavenging functions. The key to separating and purifying Ginkgo flavonol glycosides from complex Ginkgo biloba extracts is the selection of extraction methods with higher specificity and efficiency. Molecularly imprinted polymers (MIPs) have attracted considerable research interest as an alternative for flavonoids extraction in complex matrices. Herein, MIP was prepared for the specific adsorption of Ginkgo flavonol glycosides by precipitation polymerization. Polymers such as 4-vinylpyridine, acrylamide, and methacrylic acid were analyzed using UV spectroscopy under different imprinting ratios as the precursor monomers. The optimal MIP was synthesized using rutin as a template, 4-vinylpyridine as the functional monomer, and ethylene glycol dimethacrylate as the cross-linking agent with an imprinting ratio of 1:5:25. The microscopic morphological and physical properties, as well as the adsorption capacity and selectivity of the MIP, were characterized using instrumental characterization and adsorption experiments. Using the MIP, five kinds of the Ginkgo flavonols can be selectively adsorbed to a purification of 90% from natural Ginkgo biloba extract analyzing by HPLC with ultraviolet absorption detector. The enrichment of flavonol glycosides has potential value in the prevention and treatment of diseases.
研究领域:
植物化学
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高分子科学
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分析化学
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天然产物化学
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分离科学
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Haoxiang Wang,Yuyang He,Ruoyu Wang,Mengqi Lu,Yuting Luo,Yiwei Sun,Dongpo Xu
An electrochemical sensor with a dual recognition strategy was designed using molecularly imprinted polymers (MIPs) and boric acid for the highly sensitive detection of rutin. The synthesized metal-organic framework (MOFs) is combined with phenylboronic acid, which increases the recognition area of phenylboronic acid and its recognition ability towards adjacent diol compounds. Meanwhile, the molecularly imprinted polymer (MIP) provides specific recognition for rutin. The dual recognition strategy is widely adopted in sensors, enhancing the specificity of the imprinted cavity and effectively addressing the influence of interfering substances in complex matrices. After optimizing the experimental parameters, the detection range of this sensor is from 1 nM to 200 nM, and the limit of detection is 0.43 nM, indicating that this sensor has high potential for practical applications.
研究领域:
分析化学
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生物传感
·
药物分析
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高影响因子
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