RSC Advances
·
2024
·Vol. 14
·期 11
·7858-7866
·
IF 3.9
Pang Tingyuan,Liu Xiaorui,Li Jia,Song Qi,Li Junren,Han Ling,Shu Wenying,Jian Xiaoshun,Zhang Meimei
Cholesterol (CHOL) is an important clinical biochemical indicator that plays an important role in the regulation of the fluidity, permeability, and microstructure of cell membranes. Therefore, it is necessary to accurately monitor CHOL levels in biological samples for the early prevention and diagnosis of various diseases. The single-band red upconversion nanoparticle (UCNP) emits light within the optical transmission window of biological tissues, and can penetrate deeper biological tissues and cause less energy loss due to scattering and thus have higher sensitivity and accuracy. Here, using the nontoxic, sensitive, and photochemically stable 3,3′,5,5′-tetramethylbenzidine (TMB) as the quenching agent and single red UCNP as the fluorescent donor, a dual-readout colorimetric and fluorescent sensor was developed to detect CHOL. The detection mechanism and feasibility were discussed in detail, and experimental conditions such as Fe2+ concentration, TMB concentration and reaction time were explored. Under optimal conditions, the limits of CHOL detection by colorimetry and fluorescence were 0.85 μM and 0.63 μM. The sensing system was used to measure CHOL in serum samples and the values obtained by these two modes were close, and the spiked recoveries were 97.2–102.2% and 97.1–103.7%, respectively, which holds great potential in clinical diagnosis and health management.
研究领域:
生物化学
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医学诊断
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 22
·3530-3538
·
IF 2.7
Aham Emmanuel Chigozie,A. Ravikumar,Xiaofeng Yang,G. Tamilselvan,Yibin Deng,A. Arunjegan,Xuesong Li,Zhang Hu,Zhen Zhang
Biomolecules play vital roles in many biological processes and diseases, making their identification crucial. Herein, we present a colorimetric sensing method for detecting biomolecules like cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). This approach is based on a reaction system whereby colorless 3,3′,5,5′-tetramethylbenzidine (TMB) undergoes catalytic oxidation to form blue-colored oxidized TMB (ox-TMB) in the presence of hydrogen peroxide (H2O2), utilizing the peroxidase and catalase-mimicking activities of metal–phenolic coordination frameworks (MPNs) of Cu–TA, Co–TA, and Fe–TA nanospheres. The Fe–TA nanospheres demonstrated superior activity, more active sites and enhanced electron transport. Under optimal conditions, the Fe–TA nanospheres were used for the detection of biomolecules. When present, biomolecules inhibit the reaction between TMB and H2O2, causing various colorimetric responses at low detection limits of 0.382, 0.776 and 0.750 μM for Cys, Hcy and GSH. Furthermore, it was successfully applied to real water samples with good recovery results. The developed sensor not only offers a rapid, portable, and user-friendly technique for multi-target analysis of biomolecules at low concentrations but also expands the potential uses of MPNs for other targets in the environmental field.
研究领域:
生物化学
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分析化学
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纳米生物学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 44
·期 2
·381-386
·
IF 3.069
Qi Wang,Xulu Ma,Houhua Lv,Awen Wei,Tingxuan Wu,Lifeng Ding,Xiang Ma,Chunlei Ma
Ascorbic acid (AA) is an esstential micronutrient in the human body and participates in vital processes. It can only be supplemented by external intake, making the determination of AA in food or beverages of significance. In the present work, MnO2 nanoparticle (MnO2 NP) based turn-off colorimetry was established for AA determination on the basis of AA-stimulated inhibition of catalytic oxidation. MnO2 NPs were synthesized through a biotemplating method and displayed effective catalytic oxidation activity for oxidation of TMB, triggering a chromogenic reaction. Interestingly, the introduction of AA induced the decomposition of MnO2 NPs and as a consequence decreased the colour. Therefore, sensitive and selective turn-off colorimetry for AA detection was established and a satisfactory linear range of 0.5–30 μM with a detection limit of 0.28 μM was obtained. This MnO2 NP-based method was activated via a novel sensing principle, complementing and facilitating colorimetry for AA.
研究领域:
分析化学
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纳米技术
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食品科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Malin Cui,Jingdan Zhou,Yuan Zhao,Qijun Song
A facile and efficient method was developed for the preparation of iridium nanoparticles (Ir NPs) with superior peroxidase-like activity. Under the catalytic action of the Ir NPs, the peroxidase substrate 3,3,5,5-tetramathylbenzidine (TMB) can be oxidized by H 2 O 2 to form a blue-colored production oxTMB. Kinetic analysis indicated that the catalytic behavior was in accordance with the typical Michaelis-Menten kinetics and the catalytic reaction followed the ping-pang mechanism. Electron Spin Resonance (ESR) experiment showed that no hydroxyl radical formed in the reaction process. Peroxide substrates with different electron withdrawing ability (benzoyl peroxide, H 2 O 2 and artemisinin) were used to study the catalytic reactions and results indicated that H 2 O 2 can oxidize TMB with much faster rate than artemisinin and slower rate than benzoyl peroxide. These results suggest an electron transfer mechanism was involved in the system of TMB-H 2 O 2 -Ir NPs. By using TMB as the colorimetric substrate, H 2 O 2 can be rapidly determined and the method was extended for the determination of xanthine based on its production of H 2 O 2 in the presence of xanthine oxidase. The prepared Ir NPs exhibit good stability in wide range of pH and temperature. The Ir NPs retained at least 90% of their initial catalytic activity after stored at ambient temperature for three months. The high specificity for H 2 O 2 and the excellent stability of the peroxidase-like Ir NPs showed the great application potential in biotechnology field.
研究领域:
生物化学
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分析化学
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纳米材料科学
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产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Yunxia Zhuang,Xiaodan Zhang,Qiumeng Chen,Siqi Li,Haiyan Cao,Yuming Huang
Here, we report the synthesis, characterization, and oxidase-like activity of Co 3 O 4 /CuO hollow nanocage (HNC) nanocomposites. The Co 3 O 4 /CuO HNCs were successfully prepared by hydrothermal treatment of ZIF-67 in copper nitrate solution, followed by carbonization in air. The obtained nanocomposites were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. The Co 3 O 4 /CuO HNCs possess enhanced oxidase-like activity compared with pure Co 3 O 4 and CuO, and they can catalytically oxidize the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate in the absence of H 2 O 2 to produce an intense blue product. Reactive oxygen species (ROS) measurements indicate that 1 O 2 and O 2 − radicals are the major ROS in catalytic oxidation of TMB by dissolved oxygen in the TMB–Co 3 O 4 /CuO HNC system. We then developed a simple, sensitive, visual, and colorimetric biosensing method for dopamine (DA) based on its inhibiting effect on TMB oxidation. The proposed method allows for DA detection with a limit of detection of 0.027 μM and a dynamic range of 0.05–8 μM. This new colorimetric method was successfully used to detect DA in biological samples. The present work demonstrates a simple strategy to fabricate an efficient oxidase mimic with potential applications in bioanalysis and clinical diagnosis.
研究领域:
材料科学
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生物传感
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分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Xiangheng Niu,Mengzhu Wang,Hengjia Zhu,Peng Liu,Jianming Pan,Bangxiang Liu
Monitoring the level of heparin in clinical matrices is significant because of its pivotal role in preventing thrombosis. Compared to traditional single-signal sensors, multi-signal ratiometric detection can provide anti-interference results especially in complicated environments. However, fabricating an easy-to-operation, low-cost and robust sensor for the ratiometric detection of heparin still remains challenging. Here we propose a novel nanosensor for the ratiometric multicolor sensing of heparin with high performance. The sensor is based on the specific electrostatic interaction between the target and a positively charged species generated from nanozyme catalysis. FeMoO 4 nanorods are explored as an oxidase mimic for the first time, showing a high activity at neutral pH to catalyze the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to blue TMBox with an absorbance at 652 nm. Heparin can induce the rapid aggregation of the produced TMBox intermediate with rich positive charges due to their strong electrostatic interaction, leading to the formation of a purple Heparin-TMBox complex providing a signal at 565 nm. With the increase of heparin, the color changes from blue to indigo and further purple, enabling the multicolor sensing of the target. As a result, ultrasensitive determination of heparin was obtained with a very low detection limit. The fabricated nanosensor could differentiate heparin from complex species with no interferences, and it provided reliable analytical results for heparin in both serum and plasma. With robust performance, low cost and facile fabrication, the sensor holds great potential in monitoring heparin for clinical applications.
研究领域:
生物化学
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分析化学
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纳米技术
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临床诊断
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 44
·28349-28358
·
IF 4.036
Xin Zhang,Siqin Wang,Jiahao Dao,Jiajing Guo,Yanfang Gao
A novel colorimetric platform based on nano-composites of two-dimensional (2D) molybdenum disulfide nanosheets (MoS2 NSs) and one-dimensional (1D) carbon nanotubes (CNTs), called 2D–1D MoS2-CNT nanozyme, was fabricated for the selective and sensitive determination of hydrogen peroxide (H2O2) in soda water. The MoS2-CNT nanozyme was synthesized through a one-step solvothermal reduction method. The introduced CNTs could effectively prevent the stacking of MoS2 nanosheets (NSs) and not only expanded the interlayer distance of MoS2 NSs from 0.620 nm to 0.710 nm but also improved their specific surface. Under acidic conditions, the as-prepared 2D–1D MoS2-CNT nanozymes could oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-oxidized TMB (oxTMB) in the presence of H2O2, resulting in enhanced peroxidase-like (POD-like) activity. The kinetic study showed that MoS2-CNT nanozyme had stronger catalytic activity than natural horseradish peroxidase (HRP). The linear range for H2O2 colorimetric determination was 5.00–500 μmol L−1 with a limit of detection (LOD) of 1.40 μmol L−1. Furthermore, the established determination method was applied to actual samples and the recoveries of H2O2 spiked in soda water were in the range of 92.3–107%, showing feasibility for the analysis of food.
研究领域:
分析化学
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纳米材料
·
食品分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 8
·1951-1959
·
IF 3.8
Sun Shuo,Chen Changyu,Fu Xingyu,Zhang Yandong,Wu Xinyue,Hao Junkai,Feng Jing,Hu Lei,Yao Wenli,Yan Zhengquan
To conveniently monitor bioactive cysteine (Cys) and Fe 2+ in practice, a kind of poly-β-cyclodextrin strengthen praseodymium oxide (Pr 6 O 11 ) porous oxidase mimic ( p -β-CD@Pr 6 O 11 ) was constructed by virtue of the strong coordination between nano Pr 6 O 11 and poly-β-cyclodextrin substrate. After its microstructure and physicochemical property were characterized in detail, it was noted that porous p -β-CD@Pr 6 O 11 exhibited excellent enzyme-like catalytic activity to accelerate the oxidation of 3,3′,5,5,′-tetramethylbanzidine (TMB) and 2,2′-azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid) ammonium salt (ABTS) with significant color-enhancement effect in the air. Based on the signal amplification, trace Cys could exclusively deteriorate the UV-vis absorbance at 653 nm of p -β-CD@Pr 6 O 11 -TMB and Fe 2+ alter the one at 729 nm of p -β-CD@Pr 6 O 11 -ABTS with visual color changes. Under the optimized conditions, the proposed p -β-CD@Pr 6 O 11 -TMB and p -β-CD@Pr 6 O 11 -ABTS systems were successfully applied for dual-channel monitoring of Cys in Cys capsules and fetal bovine serum and Fe 2+ in agricultural products with quite low detection limits, i.e., 7.8×10 −9 mol·L −1 for Cys and 6.93×10 −8 mol·L −1 (S/N=3) for Fe 2+ , respectively. The synergetic-enhancement detection mechanisms to Cys and Fe 2+ were also proposed. Graphical
研究领域:
分析化学
·
材料科学
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生物化学
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纳米技术
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环境监测
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生物传感器
✓ 已验证
DOI
高影响因子
DALTON TRANSACTIONS
·
2020
·Vol. 49
·期 37
·13160-13166
·
IF 4.174
Yali Yu,Qingqing Zhang,Heng Gao,Chenxiao Yan,Xiong Zheng,Tong Yang,Xiaoshun Zhou,Yong Shao
G-quadruplex DNAs (G4s) have been reported to exhibit the DNAzyme activities by binding with some metal complexes and functional organic ligands. However, there is a challenge to develop metalloenzyme-mimic G4-based innate DNAzymes using the complexed metal ions directly serving as the active centers. This will diversify DNAzymes for developing novel devices since G4 structures are more polymorphic than the other DNA foldings. In this work, we found that the lanthanide trivalent cerium ion of Ce3+ can bind to the human telomere G4 (htG4) according to a 1 : 2 binding mode favorable for creating metalloenzymes-mimic G4 DNAzymes. This Ce3+-G4 entity exhibits a peroxidase activity towards the oxidation of the substrate of 3,3,5,5-tetramethylbenzidine (TMB) by hydrogen peroxide. The 5′ G4 tetrads with the orderly arranged carbonyl oxygen atoms are believed to be the coordination sites for Ce3+ and favor the conversion between Ce3+ and Ce4+. Our work provides an alternative feasibility in developing the G4-based innate DNAzymes for variant applications.
研究领域:
生物化学
·
分子生物学
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催化
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核酸化学
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DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2017
·
IF 2.334
Zhonghua Xue,Xiaofen Wang,Honghong Rao,Xiuhui Liu,Xiaoquan Lu
Many strategies have been explored for selectively and sensitively detecting cysteine in different samples. Here, a novel colorimetric sensor based on self-assembly nanostructures of Fe 3+ -H 2 O 2 /Tetramethylbenzidine system with dual-level logic gate function and colorimetric determination of cysteine were firstly explored. The proposed Fe 3+ -H 2 O 2 -TMB system provides a sensitive optical signal due to the selectively reductive ability of cysteine to the oxidized TMB and thus could be successfully applied to the construction of instant on-site visual detection method with a paper based test strip for cysteine determination in a sample solution as well as for a dual-level logic gate fabrication.
研究领域:
分析化学
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生物传感
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Qi Wang,Yongli Ding,Randy A. Dahlgren,Yue Sun,Jingjing Gu,Yuhao Li,Tingting Liu,Xuedong Wang
Total antioxidant capacity (TAC) can be evaluated by detecting the content of antioxidants , such as ascorbic acid , based on the enzyme-mimetic activity of nanomaterials . Herein, we fabricated a 3D-V 2 O 5 /NC nanocomposite using a self-templating strategy, which achieved ultrafine particles (∼2.5 nm), a porous carbon layer, large specific surface area (152.4 m 2 /g), N-doping and heterogeneous structure. The strong catalytic activity of 3D-V 2 O 5 /NC resulted from the integrated effect between the ultrafine structure of V 2 O 5 nanoparticles and the 3D porous nitrogen-doped carbon framework, effectively increasing the number of active sites. This nanozyme presented a higher catalytic activity than its components or precursors in the nanocomposite (e.g., VN/NC, NC, V 2 O 5 , and VO 2 /g-C 3 N 4 ). ROS scavenging experiments confirmed that the dual enzyme-like activity of 3D-V 2 O 5 /NC (catalase-like and oxidase-like) resulted from their co-participation of ‧O 2 − , h + and ‧OH, among which ‧O 2 − played a crucial role in the catalytic color reaction. By virtue of the 3D-V 2 O 5 /NC nanoenzyme activity and TMB as a chromogenic substrate, the mixed system of 3D-V 2 O 5 /NC + TMB + H 2 O 2 provided a low detection limit (0.03 μM) and suitable recovery (93.0–109.5%) for AA. Additionally, a smartphone-based colorimetric application was developed employing "Thing Identify" software to evaluate TAC in beverages. The colorimetric sensor and smartphone-detection platform provide a better or comparable analytical performance for TAC assessment in comparison to commercial ABTS test kits. The newly developed smartphone-based colorimetric platform presents several prominent advantageous, such as low cost, simple/rapid operation, and feasibility for outdoor use.
研究领域:
纳米材料科学
·
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 4
·1-9
·
IF 5.7
Tu Xinman,Deng Lamei,Ren Boyuan,Xu Zihan,Zhang Li
A conventional colorimetric method based on CuS-catalyzed H 2 O 2 is improved by a dual-modification strategy and employed for thioglycolic acid (TGA) determination. The doping of Co(II) can enhance ion exchange efficiency. Meanwhile, the modification of g-C 3 N 4 can increase specific surface area and decrease unspecific aggregation. The constructed g-C 3 N 4 /Co-CuS nanocomposite exhibited a favorable catalytic feature. A Michaelis constant (K m ) value of 0.02 mM has been achieved, which is 1/160 of those of CuS and horseradish peroxidase (HRP). The g-C 3 N 4 /Co-CuS displays a rapid color response in 3 min and resulted in a stable measurable signal within 10 min. In the determination procedure, the sulfhydryl contained in TGA is capable of preventing TMB oxidation via competing the · OH produced by catalysis and caused a color distinction that is related to the TGA amount. The distinctions of absorbance (λ max = 652 nm) of different concentrations of TGA are recorded. Linearity is obtained in the ranges of 2.5 – 20 µM and 20 – 160 µM, and the LOD is 0.14 µM. In the real sample assays of perm agent and Qianhu lake water, the recoveries were 96.70 – 106.84% and 100.21 – 101.90%, respectively. This demonstrates that the proposed dual-modification strategy for CuS contributes to highly efficient and convenient determination of TGA in daily cosmetics and water analysis. Graphical Abstract
研究领域:
分析化学
·
材料科学
·
环境科学
·
化妆品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）