Journal of Materials Chemistry A
·
2021
·Vol. 9
·期 43
·24308-24314
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IF 12.732
Mingyue Jiang,Yue Yuan,Yuchen Fang,Shouxin Liu,Jian Li,Zhijun Chen,Qiuying Pang,Shujun Li
Using light-harvesting materials to enhance natural photosynthesis is attracting much attention in both materials and plant research. The working efficiency of most light-harvesting materials is, however, compromised by a narrow light-harvesting range (either ultraviolet (UV) only, or near-infrared (NIR) only) and a short lifetime of the excited state. We have now developed a new light-harvesting material (UCNP@CDs) from upconverting nanoparticles (UCNPs) and carbon dots (CDs), which has good photon up- and down-converting properties, because of the UCNPs and CDs, respectively. UCNP@CDs efficiently absorbed and converted both UV and NIR light to visible light, with a long lifetime for the excited state. UCNP@CDs was incorporated into a carboxymethylcellulose (CMC) matrix to produce a light-harvesting film that enhances natural photosynthesis, both by isolated chloroplasts and by living plants. Our rationally designed UCNP@CDs/CMC composite light-harvesting film was able to enhance photosynthesis by Arabidopsis thaliana by ∼12%.
研究领域:
材料科学
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植物生物学
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生物物理学
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DOI
高影响因子
Nanoscale
·
2020
·Vol. 12
·期 26
·13948-13957
·
IF 6.895
Wenzhen Liu,Yuxiang Zhang,Wenwu You,Jianqiang Su,Shaohua Yu,Tao Dai,Yunmei Huang,Xueyuan Chen,Xiaorong Song,Zhuo Chen
Extensively drug-resistant Acinetobacter baumannii (XDR-AB) has raised considerable concerns due to its mortal damage to humans and its high transmission rate of infections in hospitals. However, current antibiotics not only show poor anti-infection effects in vivo but also frequently cause high nephrotoxicity and neurotoxicity. Herein, we report a near-infrared (NIR) light-initiated antimicrobial photodynamic therapy (aPDT) to effectively treat in vivo XDR-AB infections based on photosensitizer (PS) loaded upconversion nanoparticles (UCNPs, LiYF4:Yb/Er). Such nanoagents feature robust NIR triggered UC luminescence and high-efficiency energy transfer from UCNPs to the loaded PS, thereby allowing NIR-triggered generation of reactive oxygen species (ROS) for destroying the bacterial cell membrane. This strategy permits a high antibacterial activity against XDR-AB, resulting in a decline of 4.72 log10 in viability at a dose of 50 μg mL−1 UCNPs-PVP-RB with 980 nm laser irradiation (1 W cm−2). More significantly, we can achieve excellent therapeutic efficacy against deep-tissue (about 5 mm) XDR-AB infections without causing any side effects in the murine model. In brief, such NIR-activated aPDT may open up new avenues for treating various deep-tissue intractable infections.
研究领域:
纳米技术
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光动力疗法
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抗菌剂
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2018
·Vol. 10
·期 33
·15485-15495
·
IF 7.233
Yuxiang Zhang,Ping Huang,Dong Wang,Jincan Chen,Wenzhen Liu,Ping Hu,Mingdong Huang,Xueyuan Chen,Zhuo Chen
An alarming worldwide increase in microbial resistance to traditional drugs and classical pharmacophores has spurred the search for new antimicrobial compounds. Antimicrobial photodynamic therapy (aPDT) has recently emerged as an effective modality for the selective destruction of bacteria and other pathogenic microorganisms. However, some of the factors, including the aggregation of the hydrophobic photosensitizer (PS) in aqueous media and the inefficient biodistribution of PS limit its expansion to clinical conditions. In addition, the photoactivation under visible-light irradiation limits the therapeutic effect of aPDT for deep-tissue infection. To overcome these limitations, a PS (β-carboxyphthalocyanine zinc, CPZ) delivery system with lanthanide-doped upconversion nanoparticles (UCNPs, LiYF4:Yb/Er) and polyvinylpyrrolidone (PVP) was prepared and its antimicrobial (antibacterial and antifungal) activities were investigated. Such a near-infrared (NIR) triggered UCNPs-CPZ-PVP system significantly reduced the aggregation of CPZ and presented a high anti-infectious activity against multi-drug resistant (MDR) bacteria (methicillin-resistant Staphylococcus aureus by 4.7 log10 and MDR Escherichia coli by 2.1 log10) post aPDT (at 50 μg mL−1 UCNPs-CPZ-PVP with 0.5 W cm−2 980 nm light). In particular, UCNPs-CPZ-PVP showed high antifungal efficacy against Candida albicans. In vivo aPDT experiments were further carried out using an MDR bacterial infection murine model in the presence of 5 mm thick tissue specimens, demonstrating the great potential of UCNPs-CPZ-PVP against infections in deep tissue. Altogether, we reveal an efficient NIR-triggered nano-photosensitizer with promising antifungal and antibacterial efficacy for clinical antimicrobial therapy.
研究领域:
药理学
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微生物学
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纳米技术
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治疗学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Jingxin Mo,Xianjue Chen,Meiying Li,Wenxu Liu,Wei Zhao,Lee Yong Lim,Richard D. Tilley,J. Justin Gooding,Qinghua Li
Glioblastoma is hard to be eradicated partly because of the obstructive blood–brain barrier (BBB) and the dynamic autophagy activities of glioblastoma. Here, hydroxychloroquine (HDX)-loaded yolk–shell upconversion nanoparticle (UCNP)@Zn0.5Cd0.5S nanoparticle coating with the cyclic Arg-Gly-Asp (cRGD)-grafted glioblastoma cell membrane for near-infrared (NIR)-triggered treatment of glioblastoma is prepared for the first time. UCNPs@Zn0.5Cd0.5S (abbreviated as YSN, yolk–shell nanoparticle) under NIR radiation will generate reactive oxygen species for imposing cytotoxicity. HDX, the only available autophagy inhibitor in clinical studies, can enhance cytotoxicity by preventing damaged organelles from being recycled. The cRGD-decorated cell membrane allowed the HDX-loaded nanoparticles to efficiently bypass the BBB and specifically target glioblastoma cells. Exceptional treatment efficacy of the NIR-triggered chemotherapy and photodynamic therapy was achieved in U87 cells and in the mouse glioblastoma model as well. Our results provided proof-of-concept evidence that HDX@YSN@CCM@cRGD could overcome the delivery barriers and achieve targeted treatment of glioblastoma.
研究领域:
纳米医学
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癌症研究
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神经科学
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药剂学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 9
·612
·
IF 5.4
Zhang Ning,Hu Xuelian,Guo Shuaijie,Lu Yiwei,Tang Yiwei,Wang Shuo,Ma Tianyi
Multi-modal sensing platforms have gained significant attention due to enhanced accuracy and self-calibration capabilities, particularly within complex matrices. Targeting the demand for the real-time detection of the food spoilage marker hydrogen sulfide (H 2 S), we introduce a triple-mode sensing platform engineered via 3,4-dihydroxyhydrocinnamic-acid-directed interfacial assembly of flower-like MOF@UCNPs heterostructures that synergize intense up-conversion luminescence with potent peroxidase-mimetic activity. Sulfide selectively coordinates with the Cu 2+ nodes embedded in the MOF, concurrently (i) shutting down nanozyme catalysis, (ii) restoring up-conversion emission, and (iii) attenuating UV–vis absorbance; complementary RGB alterations captured by a smartphone furnish the third read-out. The assay delivers limits of detection of 0.06 μM (fluorescence), 0.22 μM (absorbance) and 0.90 μM (RGB imaging) with excellent linearity, and achieves satisfactory recoveries in complex food matrices. By tracking H 2 S released from meat and eggs stored at different temperatures, real-time freshness assessment is realized on a smartphone. This work not only provides a robust food freshness monitoring tool but also offers a versatile blueprint for rational design of multifunctional nanomaterials toward point-of-need analyte detection. Graphical
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2018
·
IF 3.779
Yana Liu,Cheng Zhang,Hui Liu,Yuebin Li,Zushun Xu,Ling Li,Andrew Whittaker
Rare-earth doped up-conversion nanoparticles (UCNPs) with high uniformity and dispersibility were synthesized by a facile solvothermal method. The conditions of synthesis for NaYF 4 :Yb/Tm were explored, and the NaYF 4 :Yb/Tm particles with the optimal size (about 300 nm) were obtained. Blue emission was observed with the excitation 980 nm near infrared (NIR) laser, indicating that the UCNPs can be potentially used for up-conversion luminescence (UCL) imaging. On basis of the optimal UCNPs, further coated by poly (ethylene glycol) (PEG) functionalized metal-organic frameworks (MOFs), a multifunctional platform UCNPs@MIL-PEG for cancer diagnosis and treatment was established. The core-shell structure was confirmed by TEM images. Doxorubicin (DOX) was selected as drug model and the drug loading of UCNPs@MIL-PEG was found to be 60%. Cytotoxicity indicated that UCNPs@MIL-PEG were highly biocompatible. The DOX release in different pH value revealed an excellent pH-triggered drug release. In addition, the UCNPs@MIL-PEG nanoparticles can be tracked by magnetic resonance imaging (MRI). A clear dose-dependent contrast enhancement in T 2 -weighted MR images indicated the potential act as T 2 MRI contrast agents. The UCNPs@MIL-PEG nanoparticles are expected to be simultaneously used for UCL/MR dual-mode imaging and pH-responsive drug release.
研究领域:
生物医学工程
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纳米医学
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材料科学
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肿瘤学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Jin Birui,Ma Chuan,Zhang Chuyao,Yin Huiling,Zhao Guoxu,Hu Jie,Li Zedong
There is an urgent need for a point-of-care testing (POCT) method in developing and underserved regions to distinguish between two Monkeypox virus (MPXV) clades, given their varying transmissibility and clinical manifestations. In this paper, we target the specific complement protein gene fragment of two MPXV clades and construct a high-performance upconversion nanoparticles–based lateral flow assay (UCNPs-based LFA) with double T-lines and a shared C-line. This enables qualitative and quantitative dual-mode detection when combined with a smartphone and a benchtop fluorescence analyzer. The developed LFA exhibits stable performance, convenient operation, rapid readout (within 8 min), and a much lower limit of detection (LOD) (~ pM level) compared to existing POCT methods. The proposed detection platform demonstrates significant potential for pathogen diagnosis using a POCT approach. Graphical Abstract
研究领域:
病毒学
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诊断方法
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纳米技术
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即时检测
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Hongyu Chen,Peipei Tian,Jiayi Guo,Manman Sun,Wenping Zhu,Zhendong Li,Zengchen Liu
A highly sensitive and selective upconversion near-infrared (NIR) fluorescence and colorimetric dual readout hydrogen sulfide (H 2 S) nanoprobe was constructed based on the excellent NIR fluorescence emission performance of upconversion nanomaterials (UCNPs), the specific recognition effect of synergistically synthesized gold nanoflowers (trypsin-stabled AuNFs (Try-AuNFs)) and the effective NIR fluorescence quenching capability. In this assay, the sensing strategy included three processes. First of all, the synthesized UCNPs can emit 803 nm NIR fluorescence when they were excited by 980 nm excitation light. Secondly, as a result of the principle of fluorescence resonance energy transfer (FRET), Try-AuNFs can effectively quench the NIR fluorescence of UCNPs at 803 nm, which can effectively improve the signal-to-background ratio of nanoprobes, thereby improving the sensitivity of the probes. Thirdly, in the presence of H 2 S, the Try protective layer on the surface of Try-AuNFs was specifically penetrated, which will subsequently cleave Try-AuNFs via the strong S–Au bond. As such, the NIR fluorescence of UCNPs will be restored, achieving high selectivity and sensitivity detection of H 2 S. Under optimized conditions, the linear response range of H 2 S was 0.1–300 μM, and the detection limit was 53 nM. It is worth noting that the Try on the surface of Try-AuNFs via the synergistic effect can increase the steric hindrance of the probe, and this can effectively prevent the interaction between the probe with biothiols (cysteine (Cys), homocysteine (Hcy)) and other natural amino acids (non-thiol-containing) with resultant in the high selectivity regarding the detection of H 2 S in human serum, which is unlikely to be achieved by AuNFs synthesized by the gold seed method (Se-AuNFs). This work not only provided a new type of UCNPs fluorescence quencher and recognition unit, but also exemplified that the use of the physical properties (steric hindrance) of protein ligands on the surface of nanoflowers can improve the specificity of the probe. This will provide new ideas for the design of other nanoprobes.
研究领域:
纳米技术
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分析化学
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Po Hu,Shuai Zhou,Yang Wang,Jianhua Xu,Sa Zhang,Jiajun Fu
The luminescent self-healing materials have a significant prospect in optical device applications such as biosensing, multicolor displays, and high-level anti-counterfeiting. Herein, a novel printable, room-temperature self-healing and full-color-tunable luminescent composites were developed through the noncovalent binding of physically cross-linked p(HFBM- co -SBMA)/EMITFSI and excitation-orthogonalized RGB-switchable UCNPs. According to a strategy of Selective photon capture (SPC), the RGB-switchable core-sextuple-shell UCNPs were first established for red/green/blue-switchable luminescence under three different excitations (980/808/1550 nm) and even full-color output through the combination of 980/808/1550 nm light. The copolymer p(HFBM- co -SBMA), pre-synthesized via free radical polymerization, was incorporated with ionic liquid EMITFSI to formulate room-temperature self-healing material owing to ion–dipole interactions between EMITFSI and p(HFBM- co -SBMA). Afterward, the integration of 30 wt% EMITFSI copolymer and fluothane-modified UCNPs resulted in the formation of the final full-color-tunable luminescent self-healing composites, which not only exhibited superior Young’s modulus and healing efficiency than some previously reported room-temperature self-healing materials, but also realized full-color-tunable emissions under ternary 980/808/1550 nm excitations. Significantly, the luminescent self-healing composites, which also exhibited colorless character and high transmittance, could be employed as printable inks for making various luminescent patterns (including clock, flower, and QR code) on flexible substrates via convenient and cost-effective screen-printing technique. As the results of proofs of concept, these luminescent patterns displayed fantastic viewing capability and highly secured property, which showed enormous potential of the proposed luminescent self-healing composites in flexible panchromatic display and high-security-level anti-counterfeiting. To our knowledge, this work provides the strategy for constructing full-color-tunable self-healing luminescent composites for the first time. Undoubtedly, the well-designed luminescent self-healing materials will provide new insights and guidance for exploiting the new generation of flexible optical devices, especially multicolor displays and anti-counterfeiting devices.
研究领域:
材料科学
·
光学
·
纳米技术
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显示技术
·
防伪技术
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DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 33
·13877-13887
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IF 7.394
Xiaoqian Ge,Lining Sun,Binbin Ma,Di Jin,Liang Dong,Liyi Shi,Nan Li,Haige Chen,Wei Huang
We have constructed a multifunctional nanoprobe with sensing and imaging properties by using hollow mesoporous silica coated upconversion nanoparticles (UCNPs) and Hg2+ responsive ruthenium (Ru) complex. The Ru complex was loaded into the hollow mesoporous silica and the UCNPs acted as an energy donor, transferring luminescence energy to the Ru complex. Furthermore, polyethylenimine (PEI) was assembled on the surface of mesoporous silica to achieve better hydrophilic and bio-compatibility. Upon addition of Hg2+, a blue shift of the absorption peak of the Ru complex is observed and the energy transfer process between the UCNPs and the Ru complex was blocked, resulting in an increase of the green emission intensity of the UCNPs. The un-changed 801 nm emission of the nanoprobe was used as an internal standard reference and the detection limit of Hg2+ was determined to be 0.16 μM for this nanoprobe in aqueous solution. In addition, based on the low cytotoxicity as studied by CCK-8 assay, the nanoprobe was successfully applied for cell imaging and small animal imaging. Furthermore, when doped with Gd3+ ions, the nanoprobe was successfully applied to in vivo magnetic resonance imaging (MRI) of Kunming mice, which demonstrates its potential as a MRI positive-contrast agent. Therefore, the method and results may provide more exciting opportunities to afford nanoprobes with multimodal bioimaging and multifunctional applications.
研究领域:
材料科学
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生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2020
·
IF 4.551
Di Wang,Erjun Sun
In this paper, a sensing probe (denoted as CFE) for tumor-biomarker 5-hydroxyindoleacetic acid (5-HIAA) was firstly synthesized. Its sensing mechanism was confirmed as the recovery of fluorescein structure via an ester-exchange reaction, accompanied with emission turn on effect towards 5-HIAA. To improve photostability and minimize self-quenching/aggregation of CFE molecules, they were loaded into silica based MCM-41 spheres and UCNPs-cored (up-conversion nanoparticles) MCM-41 spheres. Corresponding composite samples, denoted as CFE@MCM-41 and CFE@MCM-41@UCNPs, were carefully discussed to confirm their structure, including XRD , N 2 adsorption and desorption, SEM/TEM and TGA . The energy transfer from secondary excitation source UCNPs to probe CFE was confirmed by Forester radius calculation and emission lifetime comparison. Sensing performance of CFE@MCM-41 and CFE@MCM-41@UCNPs were finally discussed in detail. They showed emission turn on effect towards 5-HIAA with good selectivity. CFE@MCM-41 showed linear response, while CFE@MCM-41@UCNPs showed exponential response, resulting in a warning signal when analyte concentration was higher than a critical level. The reason for this exponential response was discussed and explained. Their practical sensing result for human urine test was compared to HLPC method and literature methods, which confirmed the promising result of CFE@MCM-41 and CFE@MCM-41@UCNPs.
研究领域:
生物化学
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医学诊断
·
材料科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Caiping Ding,Shasha Cheng,Cuiling Zhang,Youran Xiong,Mingqiang Ye,Yuezhong Xian
A luminescence resonance energy transfer (LRET) system was successfully developed using near-infrared (NIR) Ag2S nanodots (NDs) as the energy acceptors and upconversion nanoparticles (UCNPs) as the energy donors. The system possessing the properties of NIR excitation (980 nm) and NIR emission (795 nm) was used for the ratiometric detection and bioimaging of pH in tumor cells and zebrafish. Glutathione and mercaptopropionic acid (MPA) co-modified Ag2S NDs (GM-Ag2S NDs) were prepared by ligand exchange with an excellent pH-responsive property over a pH range of 4.0 to 9.0. The NIR GM-Ag2S NDs were covalently grafted with silica coated UCNPs, and an efficient LRET platform was developed via modulation of the thickness of the silica coating. Due to the LRET process between UCNPs and GM-Ag2S NDs, a ratiometric luminescence nanoprobe with the properties of NIR excitation–NIR emission was constructed for pH biosensing and bioimaging. On the basis of high contrast bioimaging, the nanoplatform can distinguish between tumor and normal tissue in the zebrafish model.
研究领域:
纳米技术
·
生物医学工程
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分析化学
·
生物成像
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）