TALANTA
·
2025
·
IF 6.1
Xiujuan Wang,Binghui Shi,Cengceng Xia,Mengnan Hou,Jingying Wang,Anning Tian,Chao Shi,Cuiping Ma
Traditional detection of foodborne pathogen relies on advanced analyzers, which is inadequate for the rapid control of infections, particularly in resource-limited regions, highlighting the necessity of developing detection systems for point-of-care testing (POCT). Herein, taking Vibrio parahaemolyticus as a detecting target, we reported poly- L -lysine functionalized silica membrane (PL-SM) based loop-mediated isothermal amplification (pLAMP) platform for sensitive on-site detection. This platform utilized PL-SM for DNA capture driven by the electrostatic attraction between protonated amine groups of poly-L-lysine and negatively charged phosphate groups of DNA, followed by introducing a colorimetric indicator calcein for LAMP amplification. After optimization, the colorimetric mode of pLAMP allowed the screening of V . parahaemolyticus with the visual limit of detection ( v LOD) of 1 CFU/mL in 50 min, 1000-fold lower than methods based on commercial kits. Validation was performed using 174 seafoods, which was 97 % concordant to those of real-time PCR. Furthermore, an image processing approach was developed based on the analysis of the RGB under UV light. Paired with a smartphone, the objective analytical method could be readily conducted in the field. Thus, we propose a sensitive and visual detection platform, which may play a crucial role in improving testing efficiency and accuracy in food safety, medical diagnostics, and environmental monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Shouyu Zhang,Pu Xiong,Yongsheng Ma,Ning Jin,Shibo Sun,Xiaoying Dong,Xiaodong Li,Jianqiang Xu,Hao Zhou,Weiping Xu
Insect biorefinery by black soldier fly larvae (BSFL), Hermetia illucens , has emerged as an innovative technique for the valorization of food waste. However, despite BSFL being an attractive natural source of antimicrobial proteins (AMPs), there is a scarcity of research on the antimicrobial activity and transcriptome expression of AMPs derived from BSFL following waste treatment. In the present study, food waste treatment was performed by BSFL with a substrate C/N ratio ranging from 21:1 to 10:1, marine Vibrio parahaemolyticus (VP) was selected as the model aquaculture pathogen, the antimicrobial activities of AMPs in vitro and zebrafish in vivo were examined, and the molecular mechanism of the C/N-dependent AMP difference was expounded. Findings were made that the AMP extract of C/N16:1 resulted in relatively higher antimicrobial activity in vitro than that of other C/Ns. Further, the AMPs of C/N16:1 exhibited a promising in vivo defense effect for elevating the 96-h survival rate of zebrafish from 0% to 39% after VP infection , comparable to the animal antibiotic sulfamethoxidine. The results of transcriptome analysis reveal that lysozymes were the highest expressed components in the AMP gene family. The C/N16:1 BSFL significantly up-regulated 12 out of 51 lysozyme genes compared with C/N21:1, which likely contributed to the improvement of AMP antimicrobial activity. Further, C/N16:1 significantly up-regulated the expression of lysozyme, glycosyl hydrolase and muscle protein genes compared with C/N21:1, which likely enhanced the defense ability of the immune system, the utilization of the starch-like substrate, and the mobility of the larvae, thereby facilitating the larval transformation and AMP production. Overall, such results indicate that waste C/N ratio interacted with the activity and expression of BSFL AMPs through transcriptome regulation, and the BSFL AMPs derived from food waste could be used for the defense against marine pathogens to support the sustainable development of aquaculture.
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Xin Xia,Xinjie Ji,Penglu Liu,Yi Xia,Nan Cheng
AEW 30 s kills high-load S. aureus & V. para without resistance in 10 generations. • S. aureus enriches carbon metabolism & CAMP resistance; V. para enriches QS & ABC. • AEW acts via ROS accumulation, not membrane rupture. • AEW disrupts two-component & QS of S. aureus / V. para to remove biofilm. • Blueberries require 1 min AEW; oysters need ≥3 min due to rough surfaces. Foodborne pathogens and their biofilms seriously endanger global fresh food safety, and conventional non-thermal disinfection is limited by poor biofilm removal capacity. However, the species-specific mechanisms against biofilm-embedded pathogens and the matrix-dependent efficacy on real food surfaces have not been systematically investigated for AEW. Herein, the disinfection efficiency and molecular mechanisms of residue-free acidic electrolyzed water (AEW) were explored against Staphylococcus aureus (Gram-positive) and Vibrio parahaemolyticus (Gram-negative). AEW achieved full bacterial inactivation within 30 s and showed no evidence of inducing stable inherited resistance over 10 successive generations. In practical food matrices, AEW eliminated pathogens on blueberries in 1 min, whereas over 3 min was needed for rough-shelled oysters, and mature biofilms, especially of S. aureus displayed stronger tolerance, which was further quantified via the Weibull kinetic model. Transcriptomic changes in quorum sensing, two-component, and metabolic pathways were consistent with increased reactive oxygen species (ROS) levels and biofilm disruption observed physiologically and ultrastructurally, suggesting that AEW may impair biofilm integrity and contribute to metabolic suppression in a species-dependent manner, without evident membrane lysis.With its fast disinfection and zero-residue features, AEW shows application potential for on-site decontamination of fruits and seafood in food industry. Download: Download high-res image (256KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2021
·
IF 5.277
Houqi Ning,Yu Cong,Hong Lin,Jingxue Wang
Cationic peptide chimeric lysins, Lysqdvp001-5aa, Lysqdvp001-10aa and Lysqdvp001-15aa, were designed based on lysin Lysqdvp001 from Vibrio parahaemolyticus ( V. parahaemolyticus ) phage qdvp001. These chimeric lysins showed equivalent peptidoglycan hydrolysis activities with Lysqdvp001 and could lyse the bacteria from the outside. The antibacterial activity as well as outer and inner membrane permeabilization of Lysqdvp001 and chimeric lysins against V. parahaemolyticus were Lysqdvp001-15aa>Lysqdvp001-10aa>Lysqdvp001-5aa>Lysqdvp001. Lysqdvp001-15aa exhibited an excellent antibacterial activity with minimum inhibition and bactericidal concentrations (MIC and MBC) of 0.2 and 0.4 mg/mL, respectively, and its antibacterial spectrum was much broader than phage qdvp001. Membrane hyperpolarization and membrane phospholipid exposure of V. parahaemolyticus were observed after Lysqdvp001-15aa treatments. Transmission electron microscope (TEM) showed Lysqdvp001-15aa destroyed structure integrity of V. parahaemolyticus . Besides, MIC and MBC of Lysqdvp001-15aa decreased V. parahaemolyticus counts in oyster by 3.20 and 4.03 log 10 CFU/g. Lysqdvp001-15aa at MBC eradicated about 50% of V. parahaemolyticus biofilms and inhibited over 90% of the formation of the bacterial biofilms.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CURRENT MICROBIOLOGY
·
2025
·Vol. 82
·期 4
·1-8
·
IF 2.6
Bai Yameng,Liang Tianqi,Zhao Jie,Lin Yawen,Lu Qian,Zhou Xiujuan,Yang Menghua,Zhong Xiaojun
The marine bacterium Vibrio parahaemolyticus is responsible for the seafood-borne gastroenteritis in humans. Two-component system (TCS) TtrRS plays important roles in host colonization and works as an important regulator in V. parahaemolyticus . However, the mechanisms by which TtrRS regulates the downstream genes, as well as the function of their target genes, remain largely unknown. In this study, we performed a Find Individual Motif Occurrences (FIMO) analysis using the ttrR box to scan the V. parahaemolyticus genome and identified 68 potential binding sites harboring the ttrR box. Thereinto, a ttrR box exists in the promoter region of the 05890–05900 operon. We further demonstrated that TtrR directly regulates the expression of the 05890–05900 operon by binding to the ttrR box. In addition, another TCS, 05905/05910, could assist in the regulation of TtrR to the 05890 – 05900 operon. Subsequent study showed that the 05890–05900 operon promotes the sulfur metabolism of V. parahaemolyticus at the early growth stage. Our results suggested that TtrRS and their target genes generate a complex regulatory pathway to better control sulfur metabolism in V. parahaemolyticus .Please check and confirm the edit made in the article title and amend if necessary.It's OK.
✓ 已验证
DOI
PubMed
高影响因子
METHODS
·
2019
·
IF 3.782
Hongshun Hao,Shouchen Hao,Hongman Hou,Gongliang Zhang,Yunxia Hou,Zuowei Zhang,Jingran Bi,Shuang Yan
A novel photoelectrochemical (PEC) immunosensor based on CdSe quantum dots (QDs) sensitized Ho 3+ /Yb 3+ -TiO 2 for the detection of Vibrio parahaemolyticus (VP) was assembled. The working electrode was constructed via the layer-by-layer (LBL) method with the Ho 3+ /Yb 3+ -TiO 2 , CdSe QDs, NHS/EDC, antibody of VP (anti-VP), bovine serum albumin (BSA) modified on the surface of the FTO in sequence. Ascorbic acid (AA) acts as an electron donor to combine photogenerated holes in order to provide a stable current system. Ho 3+ and Yb 3+ co-doping TiO 2 broadened the spectral response range of TiO 2 to the infrared region and improved the photocurrent responsiveness of TiO 2 . The PEC immunosensor, with Ho 3+ /Yb 3+ ratio of 1:5, Ho 3+ /Yb 3+ -TiO 2 of 2 mg/mL and PBS solution of pH 7.4, had an optimal photocurrent responsiveness. Immobilization of anti-VP was by classical NHS/EDC coupling reactions between COOH groups of CdSe QDs and NH 2 groups of the anti-VP. The results indicated that PEC immunosensors had a low detection limit of 25 CFU/mL, a wide detection range of 10 2 –10 8 CFU/mL, high stability, low price, and short detection time. This method could be promising for the rapid and ultrasensitive detection of pathogenic microorganisms in the food.
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Kuiyu Wang,Liebang Du,Liren Zhang,Xiliang Luo,Tao Yang,Hui Zeng
Nowadays, microbial visual sensing platforms based on aptamers and metal-organic frameworks (MOFs) have been reported, but most of them require complex modification processes, which undoubtedly increases the difficulty of detection. Here, we constructed modified-free magnetic-aptamer MOF (Fe 3 O 4 [email protected] ) and signal-aptamer MOF ( [email protected] ), which effectively avoided complex biological modification. The rapid sensing strategy was adopted in this method to avoid the complicated washing steps and greatly shorten the detection time. The performance of this method was verified by taking Vibrio parahaemolyticus (VP) as the mode target. The limit of detection (LOD) of VP is 10 2 CFU/mL. Furthermore, this strategy can achieve rapid visual analysis of other target objects only by changing different aptamers, and has broad application prospects in the fields of rapid detection of food pollutants, rapid medical diagnosis, environmental monitoring, etc.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Xi Jin,Liangke Gong,Jianwei Liang,Zhenhao Wang,Kuiyu Wang,Tao Yang,Hui Zeng
Vertically-ordered mesoporous silica film (VMSF) has proven to be an effective anti-interference and anti-fouling layer in the field of analysis. However, because VMSF is not stable enough in complex samples, its application in sensing field is limited. We herein use mussel-inspired multi-functional agent polydopamine (PDA) to enhance the stability of VMSF on electrode. Owing to the amino-functionalized VMSF combined with amino groups on PDA, superhydrophilicity was achieved which was proved to enhance excellent anti-fouling property. In addition, the electrodeposition of Au nanoparticles (AuNP) in the nanochannel of VMSF serving as an internal electrochemical signal shows excellent electrochemical signal stability, which provides a prerequisite for maintaining the further detection accuracy in complex samples. Finally, we validated the detection performance of the anti-fouling sensing strategy using a foodborne pathogen, Vibrio parahaemolyticus (VP), as a model target. Specifically, the aptamer was modified by covalent bond on the PDA-integrated VMSF interface surface for the recognition of VP. Using shrimp homogenate as a real sample, the anti-fouling detection performance of the aptasensor was verified. The linear range of VP detection in real samples was 10 3 -10 7 CFU/mL, and the detection limit was 10 3 CFU/mL. The results show that the anti-fouling aptasensor has excellent anti-interference and stability. It is worth emphasizing that this strategy (using inherent Au signal) enables rapid electrochemical analysis of VP without outer indicators, greatly reducing the difficulty of constructing an electrochemical VP detection strategy.
✓ 已验证
产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2026
·
IF 4.2
Le Zhang,Yihuan Chen,Yinghao Zhou,Chen Chen,Yaohuan Wang,Yiming Zheng,Shilin Shang,Maocang Yan,Xuan Dong,Qianjin Zhou,Jiong Chen
Honokiol (HKL) sensitizes Vibrio parahaemolyticus to doxycycline. • HKL–DOX shows additive interaction and reduces culturable bacteria. • HKL–DOX is associated with membrane perturbation and ATP depletion. • Dietary HKL–DOX improves shrimp survival and lowers bacterial burden. • Host and microbiota changes accompany HKL–DOX-mediated protection. Vibrio parahaemolyticus is a major bacterial pathogen in shrimp aquaculture, and the development of new anti-infective strategies is urgently needed due to limited protective options. In this study, we report that the plant-derived compound honokiol (HKL) acts as an effective adjuvant to doxycycline (DOX) against V. parahaemolyticus . Checkerboard assays revealed an additive interaction between HKL and DOX (FICI = 0.5625), accompanied by a 16-fold reduction in the minimum inhibitory concentration (MIC) of DOX from 64 μg/mL to 4 μg/mL. Plate-count-based time-kill analysis showed that the HKL–DOX combination reduced recoverable culturable bacteria to below the detection limit under the tested plating conditions. Mechanistic investigations indicated that HKL acted as an important membrane-active component of the combination, increasing bacterial membrane permeability and perturbing membrane integrity. Under combination conditions, HKL and DOX induced membrane-associated damage and intracellular ATP depletion. In a shrimp infection model, the HKL-DOX combination therapy significantly improved shrimp survival, reduced bacterial load in the hepatopancreas, partially preserved intestinal and hepatopancreatic histoarchitecture, was accompanied by changes in immune- and mucosal barrier-related gene expression and intestinal microbiota diversity and composition. Overall, HKL acts as an effective antibiotic adjuvant, highlighting a promising combination strategy for controlling V. parahaemolyticus infections in aquaculture.
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Li Wang,Mengyun Wang,Anhui Lin,Zhuozhen Qian,Yanfang Wu,Zhiyu Liu,Xiaomei Chen
Traditional methods for detecting foodborne pathogens rely on culture techniques, while advanced analytical instruments, though more sophisticated, are unsuitable for rapid detection and cannot eliminate residual contamination from secondary sources. In this study, a novel method integrating near-infrared light-induced upconversion fluorescence sensing with photothermal sterilization was developed for the detection of Vibrio parahaemolyticus ( V. parahaemolyticus ). Upconversion nanoparticles (UCNPs) were used as a fluorescence signal carrier, and silica-modified hollow polydopamine (PDA)-loaded Fe 3 O 4 @Au (HS-PDA@Fe 3 O 4 @Au) functioned as a photothermal element. Based on fluorescence resonance energy transfer (FRET) and photothermal effect, an electromagnetic-driven microfluidic fluorescence platform was constructed to achieve sensitive detection and in situ sterilization of V. parahaemolyticus . More importantly, the platform allows programmable control of the shuttling of magnetic materials within the functional compartments of the sensing chip. Under optimal experimental conditions, the detection limit for V. parahaemolyticus was 5 CFU/mL, and the linear range was 8–10 6 CFU/mL. Additionally, the sterilization rate reached 100 % after 8 min of 980 nm near-infrared irradiation, effectively eliminating the residual pathogenic bacteria and preventing secondary pollution. Therefore, the platform developed in this study demonstrates great potential for on-site detection of V. parahaemolyticus in seafood.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yuan Su,Xuetong Li,Longjiao Zhu,Huashuo Chu,Yangzi Zhang,Jingjing Tian,Shuxia Lyu,Wentao Xu
To develop an on-site, thermostatic and rapid sensor for the detection of Vibrio parahaemolyticus (V. parahaemolyticus) , A single cross-priming fluorescence (SCPF) sensor was designed using a 3D nano-nucleic acid hybrid material that termed mesoporous silica nanoparticle/nucleic acid-doped nanoflower (MSN/NA-doped nanoflower). In addition, a portable polymerase chain reaction (PCR) tube fluorescence reader was built. Further analysis of the MSN/NA-doped nanoflower morphology and the enhancement mechanism indicated that the MSNs aggregated into larger nanoclusters by adsorbing single cross-priming amplification (sCPA) components, forming MSNs/NAs-doped nanoflowers, and increasing the local concentrations to enhance sCPA efficiency. Cyclic amplification relied mainly on the self-folding hairpin-like structure of the amplified product that continuously formed, opened, re-formed, and opened again. The target DNA was detected with a detection limit of 2.4 copies/μL.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-8
·
IF 5.479
Wu Shijia,Duan Nuo,Shen Mofei,Wang Jing,Wang Zhouping
A method is described for single-step detection of V. parahaemolyticus in seafood via aptamer-based SERS. A gold-coated polydimethylsiloxane (PDMS) film was used for the enhancement of Raman scattering. The Raman reporter 4-mercaptobenzoic acid was linked to aptamer modified gold nanoparticles (AuNPs) served as a signalling probe. The negatively charged signalling probe was assembled onto the cysteamine-modified Au-PDMS film through electrostatic adsorption. On addition of V. parahaemolyticus , it will be bound by the aptamer as a biorecognition element, and this leads to the dissociation of the signalling probe from the Au-PDMS film. Hence, the Raman signal (at 1592 cm −1 ) decreases. The assay has a wide linear response that covers the 1.2 × 10 2 to 1.2 × 10 6 cfu·mL −1 V. parahaemolyticus concentration range. The detection limit is 12 cfu·mL −1 . The method was successfully applied to the determination of V. parahaemolyticus in oyster and salmon samples.
Schematic presentation of a surface-enhanced Raman spectroscopic method for single step detection of Vibrio parahaemolyticus using gold coated polydimethylsiloxane as the active substrate and aptamer modified gold nanoparticles. This solid substrate simplified the analysis procedures and enhanced the sensitivity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）