Environmental Science-Nano
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2024
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IF 5.8
Ya-Fei Cheng,Meng Li,Hai-Tian Xu,Shu-Yang Fang,Yu Zhang,Zheng-Zhe Zhang,Ren-Cun Jin
Among numerous engineered nanoparticles (NPs), CuNPs have been identified as a kind of high-risk inhibitor to anammox bacteria; however, the potential effects of salinity on the toxicity of CuNPs to anammox consortia remain unclear. Their short-term and long-term effects on anammox consortia were investigated by batch assays and continuously-fed bioreactors. The addition of 5.0–7.4 g L−1 NaCl immediately shielded the acute inhibition of 2.0–4.6 mg L−1 CuNPs on anammox activity. However, the coexistence of 5.0 g L−1 NaCl significantly aggravated the inhibitory effect of 3.0 mg L−1 CuNPs on anammox activity after exposure of about one month through reducing the content of extracellular polysaccharides. Even, the membrane permeability was significantly increased with the further increase of NaCl to 8.0 g L−1. Although the relative abundance of anammox bacteria at the DNA level was relatively higher, most of the anammox cells may not be able to perform metabolic functions normally due to membrane damage. Thus, appropriate salinity would attenuate the adverse impacts caused by the short-term shock of CuNPs, while pre-treatment is required to avoid the synergistic stress of high CuNPs when treating high salt wastewater.
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高影响因子
PROCESS BIOCHEMISTRY
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2019
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IF 2.883
Beibei Guo,Yuanhao Chen,Lu Lv,Hafiz Adeel Ahmad,Shou-Qing Ni,Liying Ren,Zhaojie Cui,Xu Fang,Zhuangming Qiao,Shaowu Ding
In this paper, three bio-reactors were designed to determine the effects of different zero-valent iron (ZVI) powder dosages on anammox. Initially, a high ZVI dosage inhibited the anammox process, while anammox adopted the habitat after long-term culture. The continuous experiment indicated that R2 (100 mg ZVI/L) had no notable effect on shortening the anammox start-up time, while R3 (1000 mg ZVI/L) reduced the start-up time from 96 to 87 days. The stable nitrogen isotope test suggested that the anammox activity of R3 was higher than that of R1 (without ZVI powder) during the stabilisation stage. The anammox growth rate increased by 20% in R3, which was verified by the real-time quantitative PCR results. In comparison to the control, the anammox quantity increased by approximately 44.5% and 54% at 100 and 1000 mg/L on day 120, respectively, which is likely due to lower dissolved oxygen and oxide-reduce potential created by the ZVI powder. The addition of ZVI may release iron ions, reduce the DO and ORP, and regulate the habitat pH conditions, which are beneficial for promoting anammox bacteria. Moreover, ZVI may directly partake in the anammox reaction pathway and improve the total nitrogen removal.
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高影响因子
ACS Sustainable Chemistry & Engineering
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2022
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IF 9.224
Meng-Wen Peng,Hui-Min Fu,Peng Yan,Peng Liu,Xun Weng,Fang Fang,Jinsong Guo,Xiaoguo Zhou,You-Peng Chen
Iron has a substantial effect on the growth and metabolism of anammox bacteria in anammox granular sludge (AnGS). Here, the interaction between ferrous ions, iron nanoparticles (FeNPs), and AnGS was examined to study the mechanism by which iron affects the spatial structure and nitrogen removal function of the AnGS system from a morphological and molecular biology perspective. The supply of 0.03 mM Fe2+ increased the live/dead bacterial ratio and the abundance of anammox bacteria. The ribosome metabolism and protein synthesis of bacteria were also promoted. However, higher microbial activity provided by 0.03 mM Fe2+ decreased the porosity and restricted the mass transfer of the AnGS, while the addition of 0.1 mM FeNPs increased the lysis and death of microorganisms by 12.57%, which led to an increase in the porosity (6.05%) and mass transfer of the AnGS. FeNPs also increased the expression of the enzymes involved in the anammox reaction (hydrazine synthetase and hydrazine dehydrogenase) and iron storage proteins. Thus, the increase in mass transfer and anammox-related enzymes jointly promoted the nitrogen removal of the AnGS. These results provided deep theoretical insights that could be used to aid the efficient and sustainable operation of the AnGS system.
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2026
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IF 3.6
Xiaojing Zhang,Zhaoyang Tian,Han Zhang,Haoran Zhao,Xinyue Xu,Qiong Wang,Yongpeng Ma
The stoichiometric ratios can indicate the nitrogen removal pathway in Anammox system. • Most of the PFOA was adsorbed by the Anammox biofilm, reaching a maximum uptake at 1 μg/L. • PFOA was bio-transformed into less toxic short-chain PFASs as predicted by ECOSAR. • Under PFOA stress, Candidatus Kuenenia excessively proliferated while hzsB gene was down-regulated. • Denitrification that reduces nitrite was enhanced with the up-regulation of nirS and nirK genes. Perfluorooctanoic acid (PFOA) is an emerging pollutant frequently detected in nitrogenous wastewater. In this study, the transformation of PFOA in an Anammox system was investigated using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and density functional theory (DFT) calculations. Additionally, the synergistic removal of nitrogen, as well as the evolution of the microbial community and functional genes in response to PFOA (at concentrations of 0.001–100 μg/L) were examined. Results showed that PFOA was primarily adsorbed into the biofilm while biotransformation also occurred, which was achieved via defluorination pathways and initiated by carboxylate-mediated decarboxylation. Several transformation products were detected, most of which exhibited lower toxicity than PFOA. 0.001 μg/L of PFOA had slight influence on nitrogen removal, whereas 1–100 μg/L continuously suppressed it. As a protective strategy, Anammox system up-regulated the growth of anaerobic ammonia-oxidizing bacteria (AnAOB). However, the gene abundance associated nitrate reduction ( nar G) and nitrite reduction ( nir S and nir K) both increased, which may hinder nitrite consumption by AnAOB. In conclusion, when treating PFOA-containing wastewater using the Anammox process, the activity of denitrifying and nitrifying bacteria should be maximally inhibited, while the biotransformation of PFOA should be enhanced. These findings provided a new perspective for the synergistic removal of PFOA and nitrogen. Download: Download high-res image (249KB) Download: Download full-size image
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2023
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IF 9.8
Xiaofang Ma,Jun Shan,Yanchao Chai,Zhijun Wei,Chenglin Li,Ke Jin,Han Zhou,Xiaoyuan Yan,Rong Ji
Microplastics (MPs) are frequently detected emerging pollutants in soil that can endanger farmland ecosystems; however, little is known about their impacts on dissimilatory nitrate reduction processes in paddy soil . Here, using the 15 N-tracer and microbial molecular techniques, we investigated the effects of MPs (200–400 μm) made of polystyrene (PS), polyvinyl chloride (PVC), and polyethylene (PE) on denitrification, anaerobic ammonium oxidation (Anammox), and dissimilatory nitrate reduction to ammonium (DNRA) and the associated microbial community in a black paddy soil. All MPs increased the Anammox rate by 6.6 %–745 % and decreased the DNRA rate by 15.1 %–74.2 %, while MPs of PS and PE significantly increased the denitrification rate by 79.3 %–102.3 % and 34.8 %–62.1 %, respectively. The MPs promoted the partitioning of NO 3 − towards denitrification and Anammox while inhibiting DNRA, as suggested by the decreased relative contributions of DNRA from 24.1 % to 5.4 %–14.2 % following MPs amendment. This was attributed to the increased denitrification gene abundance and the enriched specific denitrifier taxa, as well as the decreased DNRA gene abundance. Our findings suggest that the stimulated denitrification and Anammox by MPs, accompanied by the suppression of DNRA, may lead to substantial nitrogen loss in paddy fields , underscoring the need to further evaluate the environmental behaviors of MPs in agricultural ecosystems.
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PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2021
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IF 9.642
Jin-Jin Fu,Dong-Qi Huang,Zheng-Yang Lu,Yuan-Long Ma,Xian-Wen Xu,Bao-Cheng Huang,Nian-Si Fan,Ren-Cun Jin
Two types of anaerobic ammonium oxidation (anammox) seed sludge were selected to evaluate their responses to copper nanoparticles (CuNPs) exposure. Antibiotic-exposed anammox granules (R 1 ) were more likely to be inhibited by 5.0 mg L -1 CuNPs than the normal anammox granules (C 1 ). The nitrogen removal efficiency (NRE) of C 1 decreased by 9.00% after two weeks of exposure to CuNPs, whereas that of R 1 decreased by 20.32%. Simultaneously, the abundance of Candidatus . Kuenenia decreased by 27.65% and 36.02% in C 1 and R 1 under CuNPs stress conditions, respectively. Generally, R 1 was more susceptible to CuNPs than C 1 . The correlation analysis indicated that the horizontal transfer of antibiotic resistance genes and cop A triggered by int I1 facilitated the generation of multiresistance in the anammox process. Moreover, the potential multiresistance mechanism of anammox bacteria was hypothesized based on previous results. The results will generate new ideas for the treatment of complex wastewater using the anammox process.
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PubMed
高影响因子
CHEMOSPHERE
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2020
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IF 5.778
Weigang Wang,Yuan Yan,Junjie Wang,Yijing Zhu,Jie Ma,Zhuwu Jiang,Yayi Wang
Extracellular proteins are crucial for the formation and structural maintenance of anammox granules. However, the influence of extracellular polymeric substances (EPS) extraction methods on the secondary structure of extracellular proteins has not been studied fully. In this work, ten EPS extraction methods were applied to anammox granules and compared systematically. The EPS extraction methods were then applied to bovine serum albumin (BSA), a representative of extracellular proteins, to investigate their influence on protein secondary structure by circular dichroism (CD) spectroscopy. The ten EPS extraction methods yielded EPS concentration varying from 5.2 ± 0.1 to 84.8 ± 2.0 mg/g volatile suspended solid (VSS). Tween-20 treatment resulted in the most similar CD spectrum and protein secondary structure to the control. Treatment 0.5% Tween-20 for 4 h was found to be the best condition to preserve protein secondary structure while achieving a high EPS yield (44.4 ± 1.4 mg/g VSS) and limited cell lysis for anammox granules. This study provides an optimized protocol to extract granular EPS when the role of the secondary structure of extracellular proteins in regulating aggregation, adsorption and biofilm formation of microbes is to be elucidated.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2019
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IF 5.589
Yabing Meng,Binbin Sheng,Fangang Meng
Anammox-related processes are often applied for the wastewater treatment which contains both ammonium and antibiotics. Herein, the long-term effects of tetracycline (TC), at environmentally and industrially relevant concentrations, on the performance, anammox activity and microbial community of anammox reactors were investigated for 518 days. The control reactor (without TC exposure) was stable for nitrogen removal during the long-term operation (a nitrogen removal rate of 0.56 ± 0.05 kg-N·m −3 ·d −1 ). In the TC-added reactor, the nitrogen removal efficiency increased slightly at low TC levels (1–100 μg/L), whereas poor anammox performance occurred at high TC concentration (1000 μg/L). Furthermore, the concentrations of extracellular polymeric substances (EPS) were much higher at 10 μg/L than those in the control reactor ( P < 0.01), whereas rapidly decreased at 1000 μg-TC/L. Furthermore, the reactor performance was highly consistent with the variations of the heme c contents. Consistently, exposure to TC changed the abundance of anammox bacteria, e.g., an increase in Candidatus Jettenia abundance occurred from 2.20 ± 0.97% (0–10 μg/L) to 12.13 ± 1.66% (100 μg/L). Similarly, the genus Denitratisoma , the most predominant denitrification bacteria, also had a higher abundance at a TC concentration of 100 μg/L (15.60 ± 6.42%) than other TC concentrations (5.40 ± 2.50% and 7.65 ± 0.55% at concentrations of 10 and 1000 μg/L, respectively). The results can explain why the exposure of anammox bacteria to a lower TC concentration (100 μg/L) resulted in a better nitrogen removal rate. In contrast, exposure to a high TC level (1000 μg/L) led to a decline in the abundance of anammox bacteria and denitrifiers (1.53 ± 0.64% and 8.18 ± 0.63%, respectively) but an increased abundance in the nitrifier population (8.07 ± 1.21%; P < 0.01). Therefore, this study can aid in the design and operation of anammox-based processes treating sewage and industrial wastewater .
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PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2019
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IF 5.589
Zhen Bi,Wenjing Zhang,Ge Song,Yong Huang
Anammox bacteria are chemolithotrophic organisms growing on the conversion of ammonium and nitrite with bicarbonate as the sole carbon source. Meanwhile, anammox bacteria display a metabolic versatility to sustain their metabolism. However, there is less attention on the Fe 0 /Fe 2+ -dependent autotrophic denitrification by anammox consortia. In this study, the autotrophic nitrate reduction using different valence of iron (Fe 0 , Fe 2+ and Fe 0 + Fe 2+ , respectively) as electron donors by anammox consortia were firstly explored in continuous feeding mode. Results revealed anammox consortia showed high adaptability to the niche wherein containing nitrate and iron. They could generate nitrite and ammonium from iron-dependent nitrate reduction, and hence support their central metabolism. During 60-days operation, the maximum nitrate and total nitrogen removal efficiency reached 88.43% and 80.77%, respectively, with coexistence of Fe 0 and Fe 2+ . The expression of key functional genes involved in nitrate reduction (including narG, napA and nrfA ) in 16S rRNA level revealed the coupling of dissimilatory nitrate reduction to nitrite, dissimilatory nitrite reduction to ammonia (DNRA), and anammox processes possibly play pivotal role in nitrogen loss under Fe 0 /Fe 2+ condition. Meanwhile, abiotic reduction by Fe 0 /Fe 2+ also contributed nitrate reduction to provide nitrite and ammonium for anammox consortia. Activities of two vital enzymes hydrazine dehydrogenase (HDH) and nitrate oxidoreduetase (NAR) also inferred higher microbial activities with co-existence of Fe 0 and Fe 2+ . The present study confirms and further extends the versatile metabolisms of Anammox consortia, also it can help to circumvent the accumulation of nitrate produced by anammox process itself and increase the quality of discharge.
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高影响因子
BIORESOURCE TECHNOLOGY
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2017
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IF 5.651
Zheng-Zhe Zhang,Jia-Jia Xu,Zhi-Jian Shi,Yu-Hui Bai,Ya-Fei Cheng,Hai-Yan Hu,Ren-Cun Jin
Given the increasing use of nanoscale zero-valent iron (NZVI) particles for environmental remediation and wastewater treatment, their potential impact on anaerobic ammonium oxidation (anammox) bacteria was investigated in this study using anammox sludge. Batch assays showed that NZVI concentrations up to 200 mg L −1 did not affect anammox activity, reactive oxygen species production, and cell membrane integrity. The nitrogen removal efficiency of the continuous-flow reactor fluctuated in the presence of 20 or 50 mg L −1 NZVI, but it could return to normal over time, even at 200 mg L −1 NZVI. 16S rDNA-based high-throughput sequencing indicated that although the presence of 10, 20, 50, and 200 mg L −1 NZVI to some extent affected microbial composition, the anammox bacteria ( Candidatus Kuenenia ) never lost its dominance. The abundance of gene families that are related to the assimilation and utilization of iron was down-regulated in response to the stress of high-level NZVI.
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PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Xingyao Ye,Xiaojun Niu,Ling Li,Mengyu Lv,Dongqing Zhang,Deye Chen,Yu Line,Zhiquan Yang
N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is an antioxidant commonly used in tire manufacturing, and its release into the environment has significantly increased due to rapid urbanization. When subjected to ozonation, 6PPD converts into the harmful pollutant 6PPD quinone (6PPDQ). These substances enter wastewater treatment plants (WWTPs) via stormwater runoff and pipelines, posing significant risks to the functional microorganisms. Anammox, a strictly controlled and sensitive microbial nitrogen removal process, is especially susceptible to the effects of the pollutants. This study investigates the comprehensive impact of 6PPD-Q and 6PPD on anammox communities based on characterization analysis and metagenomics. At environmental concentrations, 6PPD-Q at 200 ng/L-1000 ng/L led to the disintegration of anammox granules. Extended exposure to both 6PPD-Q and 6PPD significantly reduces the population of anammox bacteria (AnAOB). By utilizing organic matter from dead cells and incoming carbonate as a carbon source, the system evolved into a nitrogen metabolism network primarily focused on denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This transformation was accompanied by a reshuffling of the microbial community and associated genes, resulting in an accumulation of NH 4 + -N. These findings underscore the toxicity of 6PPD-Q and 6PPD to anammox and stress the importance of incorporating 6PPD into regulatory and preventive strategies.
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PubMed
高影响因子
Journal of Cleaner Production
·
2024
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IF 9.7
Jia-Min Zhou,Ying-Jun Sun,Zhi-Han Xi,Zu-Gang Liao,Xin Ma,Ze-Tong Feng,Xin-Tao Liu,Xin-Rui Zhang,Ying Wang,Ying Chen,Ren-Cun Jin,Qian-Qian Zhang
The implications of zero-valent iron (ZVI) on performance of partial denitrification (PD) coupled with anammox process (PD-anammox) and microbial community structures were assessed in present investigation. The results indicated that removal efficiencies of nitrate nitrogen, ammonium nitrogen and total nitrogen (TN) reached 86.28 ± 0.04%, 81.05 ± 0.06% and 82.90 ± 0.80%, respectively, with 30-day addition of ZVI under ratio of ZVI to nitrate (ZVI/N), carbon to nitrate (C/N) respectively for 3 and 2.3. Moreover, the content of extracellular polymer substance elevated by ZVI, and which could substitute carbon sources, mediated electron transfer and promoted nitrogen removal efficiency. Additionally, microbiological analysis revealed that ZVI enhanced nitrogen removal process by promoting the growth of genus Ignavibacterium and Denitratisoma , which coexist with anaerobic ammonia oxidation bacterium (AnAOB) and also induced other nitrogen removal process such as heterotrophic nitrate reduction to ammonium (DNRA), nitrate-dependent anaerobic ferrous oxide (NAFO), and iron-based anammox (Feammox) process. This study offers new insights into the enhancement of the nitrogen removal process by ZVI in PD-anammox process.
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高影响因子
危险品化学品经营许可证（带存储）