BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Di Hu,Ruru Xu,Yating Jin,Shuqing Sun,Jing Ye,Jiajia Wu,Zhiyuan Dai,Jia-Wei Shen,Yanbin Lu
In this study, a sustainable and environmentally friendly method was developed for the enrichment and purification of phycocyanin from Spirulina platensis . This was achieved by utilizing a temperature-sensitive polymer, Pluronic F68, in an aqueous two-phase solvent system. The phase behavior of the temperature-sensitive polymer-based biphasic system was evaluated. The extraction conditions were optimized by both single-factor experiments and response surface methodology. Under the optimal conditions, the upper polymer-rich phase was recycled for sustainable phycocyanin extraction, resulting in a grade of 3.23 during the third extraction cycle. Pluronic F68 could be efficiently recovered and reused during the extraction process. The interaction mechanism between Pluronic F68 and phycocyanin was systematically studied using FT-IR and fluorescence analysis. This was further complemented by static and dynamic calculation of molecular motion through molecular docking and molecular dynamics simulation, indicating that hydrophobic segment of Pluronic F68 played a key role in the binding process with phycocyanin.
研究领域:
生物技术
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环境科学
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化学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2016
·
IF 1.835
Yang Lu,Miao Yu,Zhenjiang Tan,Yongsheng Yan
Polyoxyethylene(20) cetyl ether (C56H114O21, POELE20) and three sulfate salts (Li2SO4, ZnSO4, and MgSO4) were used as phase-forming material of the aqueous two-phase system, and the binodal data was measured at the three temperatures (288.15, 298.15, 308.15 K). Three classic experienced formulas were used to fit the binodal data, and they had better performance in data association. The tie-line data of studied systems were measured and fitted by the empirical formulas, i.e., Othmer–Tobias and Bancroft equations. It has better performance in data association. For the investigated systems, the influence of the salt type on the binodal curves was studied by the Gibbs free energy of ions hydration and effective excluded volume, and the influence of temperature on the tie-lines and binonal curves was also discussed. First, as it is confirmed, when the salts are of the same anion, salts with higher cation valence will have stronger abilities of salting out; the salting out abilities of salts strengthen with the increasing absolute value of Gibbs free energies of cations. Second, the salting-out abilities of salts enhance with the increase in system effective excluded volume in the investigated systems. Third, the studied aqueous two-phase systems are more prone to formation at the higher temperature than those at lower temperature. Finally, for the tie-lines in the studied systems, the absolute value of slope decreases with the temperature falling.
研究领域:
化学
·
生物化学
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DOI
高影响因子
Journal of Cleaner Production
·
2017
·
IF 5.715
Tong Wang,Cheng-Bo Gu,Sui-Xin Wang,Ping Kou,Jiao Jiao,Yu-Jie Fu
Recently, integrated and sustainable methods for extraction, transformation and purification of value-added compounds have gained increasing attention. In the present study, ionic liquid (IL)-acid based pH-dependent aqueous two-phase system (ATPS) was developed for simultaneous transformation psoralic acid-glucoside into psoralen, extraction and purification of psoralen from fig leaves for the first time. The cleaner [Bmim]Br-citric acid mixture showed enhanced transformation and extraction efficiency. The extraction yield of psoralen by [Bmim]Br-citric acid mixture was 1.45, 2.45 and 3.68 times higher than that of by [Bmim]Br-water, ethanol-critic acid and ethanol, respectively. The cleaner [Bmim]Br-citric acid based ultrasound extraction and transformation process was optimized using response surface method (RSM). Under optimum conditions, the maximum extraction yield of psoralen was 31.22 mg/g. Subsequently, we investigated and optimized the pH-dependent aqueous two-phase process. Under optimum conditions, the maximum recovery yield of psoralen was 96.34%. These results indicated that our method was efficient and had great potential for producing psoralen from fig leaves in large scale. What's more, this method could also been used to produce value-added compounds from other raw materials.
研究领域:
植物化学
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药物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Lu Liu,Yuyu He,Zixin Li,Qiutong Wan,Bing-Zheng Li,Shiyin Guo,Qingming Li,Huiping Xia
In aqueous two-phase systems (ATPS), how to control precisely the particle size of SMs remains challenging, particularly the role of the continuous phase and starch structural properties has not been thoroughly investigated. This study aimed to regulate the particle size of SMs prepared in aqueous two-phase system (ATPS) by modifying the continuous phase and acid hydrolysis time of waxy potato starch. The considered continuous phase was polyethylene glycol (PEG) and polyvinylpyrrolidone (PVP), and the starches were treated with varying the duration of acid hydrolysis. After 8 h of acid hydrolysis, the molecular weight of starch reduced from 1.45×10 7 Da to 3.03×10 5 Da. The resulting particle size of SMs exhibited 10.6 μm in the PVP system, compared to 30 μm in the PEG system. Additionally, SMs prepared in PVP system showed higher crystallinity, short-range order, enthalpy, and pasting temperature, along with smaller particle sizes, for the same acid hydrolysis time. BSA loading tests indicated that SMs with smaller particle sizes had higher drug loading capacity, with PVP-10h showing more than twice the capacity of PEG-10h. The study enabled the researcher to select the appropriate continuous phase and acid hydrolysis time for preparing SMs based on desired physicochemical properties.
研究领域:
生物材料
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药物递送
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高分子科学
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淀粉化学
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药剂学
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Yuehua Chen,Huiyong Wang,Yuanchao Pei,Jianji Wang
It is significant to develop sustainable strategies for the selective separation of rare earth from transition metals from fundamental and practical viewpoint. In this work, an environmentally friendly solvent extraction approach has been developed to selectively separate neodymium (III) from cobalt (II) and nickel (II) by using an ionic liquid-based aqueous two phase system (IL-ATPS). For this purpose, a hydrophilic ionic liquid (IL) tetrabutylphosphonate nitrate ([P 4444 ][NO 3 ]) was prepared and used for the formation of an ATPS with NaNO 3 . Binodal curves of the ATPSs have been determined for the design of extraction process. The extraction parameters such as contact time, aqueous phase pH, content of phase-formation components of NaNO 3 and the ionic liquid have been investigated systematically. It is shown that under optimal conditions, the extraction efficiency of neodymium (III) is as high as 99.7%, and neodymium (III) can be selectively separated from cobalt (II) and nickel (II) with a separation factor of 10 3 . After extraction, neodymium (III) can be stripped from the IL-rich phase by using dilute aqueous sodium oxalate, and the ILs can be quantitatively recovered and reused in the next extraction process. Since [P 4444 ][NO 3 ] works as one of the components of the ATPS and the extractant for the neodymium, no organic diluent, extra etractant and fluorinated ILs are used in the separation process. Thus, the strategy described here shows potential in green separation of neodymium from cobalt and nickel by using simple IL-based aqueous two-phase system.
研究领域:
分离科学
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绿色化学
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湿法冶金
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稀土元素回收
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离子液体
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环境工程
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
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IF 3.716
Juan He,Junfen Wan,Ting Yang,Xuejun Cao,Lin Yang
Aqueous two-phase system (ATPS) has great potential in industrial applications of bio-separations and bio-reactions. However, its large-scale application is limited by recovery difficulty of phase systems. In this paper, a recyclable ATPS was prepared by two pH-responsive copolymers (P ADB4.99 and P MDM7.08 ) and applied for purification of porcine circovirus type 2 Cap protein fermentation broth (PCV2 Cap protein). Phase separation mechanism was studied by using low-field nuclear magnetic resonance (LF-NMR). The results showed that relatively desirable ATPS were formed successfully when two copolymer concentrations were 4%–6% (w/w) with pH 7.5–8.6. The main parameters, such as copolymer concentration, temperature, pH, type and concentration of salts, were investigated. The results demonstrated that the best ATPS consisted of 4% (w/w) P ADB4.99 and 6% (w/w) P MDM7.08 , the optimal partition coefficient (K) and extraction recovery (ER) of PCV2 cap protein were 0.25 and 94.2% in the presence of 50 mM Li 2 SO 4 , 3.53 and 93.4% in the presence of 40 mM KCl, respectively, at 30 °C, pH 8.1. The maximum purity of PCV2 Cap protein was 88.4%. In addition, the two phase-forming copolymers could be recycled by adjusting solution pH to their isoelectric points (pI), with recoveries of over 97.5%.
研究领域:
生物分离技术
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病毒学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 94
·期 9
·2917-2927
·
IF 2.659
Zhuo Sun,Yang Lu,Lina Zhu,Wei Liu,Yue Qu,Nan Lin,Ping Yu
BACKGROUND Fluoroquinolone antibiotics and their metabolites can accumulate in soil and water. Trace amounts of fluoroquinolone antibiotic residues in the environment necessitate the pretreatment of samples prior to analysis. In this work, a polyoxyethylene cetyl ether (POELE20)–NaH 2 PO 4 aqueous two-phase system (ATPS) was combined with high-performance liquid chromatography (HPLC) to separate and determine trace quantities of ciprofloxacin (CIP), norfloxacin (NRF) and pefloxacin (PEF) in environmental samples. RESULTS Single-factor, multi-factor, reproducibility and accuracy experiments were performed. The extraction efficiencies and enrichment factors for the three antibiotics were above 90 and 13.8%, respectively, under optimal conditions. The recovery efficiency of CIP was 92.08–99.50%, with a relative standard deviation (RSD) of 0.60–6.34%, and the recovery efficiency of NRF and PEF was 93.40–99.30%, with RSD of 0.98–5.16%, when this method was applied to spiked water and soil samples. The RSDs of intra- and inter-day analyses were 1.56–3.42 and 1.33–3.97%, respectively. CONCLUSIONS This study demonstrated that POELE20–NaH 2 PO 4 ATPS coupled with HPLC was suitable for the simultaneous separation, concentration and determination of trace quantities of CIP, NRF and PEF in environmental samples. Moreover, this method can be applied for the determination of trace fluoroquinolone antibiotics in food samples. © 2019 Society of Chemical Industry
研究领域:
环境科学
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分析化学
·
药物分析
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产品关联
DOI
高影响因子
RENEWABLE ENERGY
·
2022
·
IF 8.634
Shanshan Tu,Xiaojie Yu,Qinghua Ji,Qiannan Ma,Cunshan Zhou,Li Chen,Clinton Emeka Okonkwo
With the deepening of the research on the conversion of cellulose biomass to furfural, there is no green and efficient method to separate sugars and 5-HMF. This study utilized a thermoreversible aqueous two-phase system (ATPS) coupled with tetrabutylammonium bromide ([N 4444 ]Br)- based deep eutectic solvents (DES) and inorganic salts for separating glucose and 5-HMF. The thermal characteristics of DESs were analyzed by thermogravimetry (TGA) and differential scanning calorimetry (DSC). The thermoreversible phase behavior of ATPS was determined by phase diagram. Furthermore, the process factors were optimized, such as the type and concentration of DES and phosphate, the temperature, and the time of ATPS were investigated, to maximize the separation of glucose and 5-HMF. Finally, the ATPS was a successful integrated catalytic reaction of glucose involving CrCl 3 and 5-HMF separation. The results showed that the ATPS consist of 30 wt% [N 4444 ]Br-EG and 25 wt% K 2 HPO 4 had the most efficient extraction rate, 5-HMF reached 96.0 ± 0.6%, and mainly existed in the DES phase, while glucose was enriched in salt phase, and the extraction rate reached 99.9 ± 0.03%. And the 5-HMF yield reached 25.6 ± 1.1% in the production separation integrated platform. This thermoreversible ATPS may provide a guide for the effective extraction of furfural from biomass-derived glucose.
研究领域:
生物化学
·
绿色化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Xiao-Hua Zhang,Jing-Jing Zheng,Xiang-Dong Qing,Fang Lin,Yan-Ting Yuan,Kai-Long Yang,Jing-Zhe Zhang,Hui-Wen Gu
The phenolic compounds are the primary ingredients contributing to the color, fragrance, and health care functions of tea. The present study sought to extract the phenolic compounds from Chinese tea samples using a novel compound salt aqueous two phase system (ATPS) with iso -propanol and two inorganic salts (sodium citrate and ammonium sulfate). The results showed that the analytes were mainly distributed in the alcohol-rich top phase within the recovery range of 82.54–110.73%. Additionally, the alternating trilinear decomposition (ATLD) algorithm combined with HPLC-DAD was employed for the simultaneous rapid quantitative analysis of six phenolic compounds in four Chinese tea samples within 7.0 min. Based on the quantitative data obtained by the proposed ATPS-ATLD method, the principal component analysis (PCA) and hierarchical cluster analysis (HCA) methods were adopted to classify and analyze the four types of Chinese teas. Overall, the proposed ATPS-ATLD method could be a potential approach for distinguishing the types of Chinese teas.
研究领域:
食品科学
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分析化学
·
植物化学
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DOI
高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Ying Huang,Diyun Chen,Shuqi Chen,Minhua Su,Yongheng Chen,Yixiong pang,Gutha Yuvaraja
Environmental thallium (Tl) and uranium (U) pollution have attracted great research attention. This study developed a stepwise extraction process to separate Tl and U in an aqueous two-phase system (ATPS) based on 15% polyethylene glycol (PEG6,000) and 10% ammonium sulfate with hydrochloric acid and chlorophosphonazo III (CPA III). The effects of PEG molecular weight , temperature, hydrogen peroxide, HCl, pH, CPA III, equilibrium time, ionic strength , and dissolved organic matter (DOM) on Tl and U separation were studied systematically. The results revealed that 10% H 2 O 2 and 3.2 M HCl in the ATPS rapidly yielded a Tl extraction rate of 95.2%, and the separation factor of Tl and U exceeded 2000 in 5 min; 97.8% of U was extracted in an ATPS containing 0.1 mM CPA III at pH 5–6, and the separation factor of U and Tl reached 5000 in 10 min. The increase of Na + and NH 4 + from 0 to 60 mM did not affect U extraction but promoted Tl extraction and separation, whereas K + inhibited Tl and U extraction. Moreover, although DOM inhibited Tl extraction, it promoted U extraction and separation. Mechanism analysis indicated that Tl and U extraction in the ATPS was due to the salting-out effect of different anions and complexing agents in different acidities. Relative to the conventional method of organic solvent extraction for Tl and U, the method developed herein has greater safety, lower cost, and more environmental benefits, which can be function appropriately in seriously contaminated wastewater containing Tl and U.
研究领域:
环境科学
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2019
·
IF 4.561
Lianglei Liu,Danyu Sun,Fenfang Li,Shaoping Ma,Zhijian Tan
It was widely reported that the heterocyclic ionic liquids (ILs) had relatively high biotoxicity and the cholinium amino acid ionic liquids (ChAAILs) were much environmentally friendly. Therefore, the ChAAILs/salt aqueous two-phase systems (ATPSs) were developed for the chiral resolution of valine enantiomers in this study. The copper complexes were prepared by ChAAILs and Cu(Ac) 2 , which can be used as both the phase-forming components and the chiral selectors. A series of factors affecting the enantioseparation result were investigated, including type and concentration of ChAAILs, molar ratio of ChAAIL and Cu(Ac) 2 , type and concentration of salt, concentration of valine, and operation temperature. The valine enantiomers were preferentially recognized in the ChAAIL-rich phase (top phase) and the maximum e.e. value of 55.6% was obtained in the top phase. This ATPS used for the enantioseparation of valine enantiomers has the advantages of environmental friendliness, high enantioseparation efficiency and simple operation by one step extraction.
研究领域:
化学
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Qing Lian,Jiamei Chen,Shijian Huang,Yiying Zou,Jiyuan Yao,Mingliang Jin,Lingling Shui
Microcapsules have emerged as versatile microcarriers in the biomedical and biological fields. Herein, we successfully fabricated polyelectrolyte microcapsules (PEMCs) with favorable release and storage characteristics through an integrated approach utilizing an aqueous two-phase system (ATPS) with microfluidic techniques. The PEMCs were synthesized at the interface between the dispersed and continuous phases by layering oppositely charged polyelectrolytes: poly(diallyldimethylammonium chloride) (PDDA) and polystyrene sodium sulfate (PSS). To improve the robustness, storage potential, and biocompatibility of the microcapsules, cellulose nanocrystals (CNCs) were integrated into the microcapsule matrix. The resulting microcapsules, which encapsulated FITC-labeled dextran (FITC-Dex), displayed tunable release characteristics in different poly(ethylene glycol) and phosphate-buffered saline solutions, suggesting that CNCs-PEMCs could be controlled by osmotic pressure and pH. Additionally, horseradish peroxidase (HRP), a model enzyme, had preserved catalytic function within the PEMCs for over 24 h, demonstrating that the microcapsules do not significantly affect enzymatic activity. The formation of a cohesive shell by integrating of CNCs with polyelectrolytes under neutral conditions shows the potential of these CNCs-PEMCs for broader applications.
研究领域:
生物医学工程
·
材料科学
·
酶学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）