ANALYTICAL CHEMISTRY
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2024
·
IF 6.7
Bin Liu,Tian Wang,Dehui Qiu,Xinrong Yan,Yuan Liu,Jean-Louis Mergny,Xiaobo Zhang,David Monchaud,Huangxian Ju,Jun Zhou
Hemin/G-quadruplex (hG4) complexes are frequently used as artificial peroxidase-like enzymatic systems (termed G4 DNAzymes) in many biosensing applications, in spite of a rather low efficiency, notably in terms of detection limits. To tackle this issue, we report herein a strategy in which hemin is chemically modified with the amino acids found in the active site of parent horseradish peroxidase (HRP), with the aim of recreating an environment conducive to high catalytic activity. When hemin is conjugated with a single arginine, it associates with G4 to create an arginine–hemin/G4 (R-hG4) DNAzyme that exhibits improved catalytic performances, characterized by kinetic analysis and DFT calculations. The practical relevance of this system was demonstrated with the implementation of biosensing assays enabling the chemiluminescent detection of G4-containing DNA and colorimetry detection of the flap endonuclease 1 (FEN1) enzyme with a high efficiency and sensitivity. Our results thus provide a guide for future enzyme engineering campaigns to create ever more efficient peroxidase-mimicking DNA-based systems.
研究领域:
生物化学
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分子生物学
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化学生物学
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Ze Xu,Jun Gao,Hongyu Zhang,Guojun Zheng,Jihong Hu,Junyao Li,Laidi Xu,Yong Kong
A thermosensitive hydrogel based on 6-deoxy-6-arginine grafted chitosan (DAC) and poloxamer 407 (P407) is prepared for wound repair. Since P407 is a thermosensitive polymer, the resultant DAC/P407-gel exhibits thermo-responsive sol–to–gel phase transition behaviors with a phase transition temperature (T sol/gel ) of ∼ 26.5 °C, making it an appropriate material for wound healing. The DAC/P407-gel has a three-dimensional (3D) network structure with large amounts of pores, and the good air permeability of the hydrogel makes it an attractive candidate as wound dressing. The introduction of DAC can not only increase the mechanical strength of the hydrogel but also significantly enhance its biological indexes including antibacterial activity, blood-clotting index (BCI) and hemocompatibility. The cytotoxicity test reveals that the developed DAC/P407-gel also shows outstanding cytocompatibility.
研究领域:
生物材料学
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医学工程
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药剂学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Xiaqing Wu,Yan Cheng,Runxiao Zheng,Keqiang Xu,Jiao Yan,Panpan Song,Yanjing Wang,Abdur Rauf,Yue Pan,Haiyuan Zhang
Tumor-associated macrophages (TAMs) of M2 phenotype have mediated the immunosuppression in a tumor microenvironment, facilitating the escape of tumor cells from immunosurveillance. Reprograming the immunosuppressive M2 TAMs to immunostimulatory M1 phenotype can activate the antitumor immune responses for cancer immunotherapy. Herein, hollow iron oxide (Fe3O4) nanoparticles (NPs) were employed to reprogram M2 TAMs toward M1 TAMs, aiming to release proinflammatory cytokines and recruit T cells to kill tumor cells. After loaded with l-arginine (l-Arg) and sealed with poly(acrylic acid) (PAA), hollow Fe3O4 NPs were fabricated into LPFe3O4 NPs, which could release l-Arg based on pH-responsive PAA and produce nitric oxide (NO) with the help of nitric oxide synthase (iNOS) overexpressed by M1 TAMs, as a result of additional tumor elimination for gas therapy. In vitro and in vivo studies demonstrate that LPFe3O4 NPs could effectively reprogram M2 to M1 macrophages, activating T cells, releasing TNF-α, and producing high levels of NO, leading to synergistic tumor therapy.
研究领域:
癌症研究
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纳米技术
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免疫学
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药学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Yang Zhou,Zhipeng Gu,Jie Liu,Keqing Huang,Guiting Liu,Jun Wu
Bone transplantations are still facing many serious challenges, hydrogel as a new kind of artificial bone substitutes has developed into a promising bone scaffold material. However, it is still a challenge to combine bioactive agents and hydrogel matrix to promote osteoinductivity. Herein, we developed a novel bioactive hydrogel based on arginine-based unsaturated poly (ester amide) (Arg-UPEA) and methacrylated hyaluronic acid (HA-MA) via photo-crosslinking. As the results indicated, we found that the introduction of Arg-UPEA into HA-MA hydrogels could finely modulate their compressive modulus, swelling level and porous structure. Besides, among groups of different feed ratio, groups of 10 % and 15 % of Arg-UPEA content effectively enhanced osteogenic differentiation in osteoblasts when compared with HA-MA hydrogel. Furthermore, better bone regeneration and expression of osteogenesis-related factors in vivo also verified the Arg-UPEA/HA-MA hybrid hydrogels as a promising scaffold material for bone tissue engineering.
研究领域:
生物材料
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组织工程
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2016
·Vol. 9
·期 2
·282-286
·
IF 1.915
B. Liu,X. P. Wang,X. Y. Sun,C. X. Yang,P. Li
In this paper, a novel fluorescent immunosensor based on self-assembled multilayers was constructed to detect microcystin-(leucine–arginine). CdTe quantum dots were firstly assembled on the quartz substrates. Then bioconjugates were formed by an amidation reaction between CdTe quantum dots and microcystin-(leucine–arginine) antibodies. The sensing system exhibited a linear range from 0.0001 to 3 μg L−1 for microcystin-(leucine–arginine) with a detection limit of 0.0440 ng L−1, 2–4 orders of magnitude lower than most other fluoroimmunoassays. Desirable results were also obtained in natural lake water and interference experiments. The fluorescent immunosensor based on self-assembled multilayers has great potential for the rapid, sensitive detection of trace amounts of microcystin-(leucine–arginine) in real water samples.
研究领域:
环境科学
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分析化学
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生物医学工程
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产品关联
DOI
高影响因子
Food Science & Nutrition
·
2019
·Vol. 7
·期 5
·1677-1684
·
IF 1.747
Chengli Hou,Xuan Song,Zheng Li,Wenting Wang,Qingwu Shen,Dequan Zhang
To increase the color stability of hemoglobin (Hb) powder, the technological conditions for arginine–hemoglobin (Arg-Hb) powder preparation were optimized by response surface methodology and the influence of arginine (Arg) on the color stability of Hb powder was evaluated. Results showed that: (a) Arg-Hb powder had better colors (less MetHb% and higher a* value) than Hb powder ( p < 0.05); (b) using MetHb% as an indicator, the optimal conditions to prepare Arg stabilized Hb were Arg concentration of 10.5 mg/ml Hb extract, reaction pH of 10.75, and reaction temperature of 18°C; (c) pH and NaCl had a significant influence on the color stability of Hb ( p < 0.05). At various NaCl concentrations and pH conditions, Arg-Hb solution showed better color than Hb ( p < 0.05); (d) Arg-Hb powder had higher a* values and higher percentages of deoxyhemoglobin and oxyhemoglobin but lower MetHb% than Hb powder during storage ( p < 0.05).
研究领域:
食品科学
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生物化学
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制药技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2013
·
IF 3.473
Shufeng Shen,Xiaoxia Feng,Ruihong Zhao,Ujjal Kumar Ghosh,Aibing Chen
The absorption of carbon dioxide (CO 2 ) into aqueous 35 wt.% equivalent potassium carbonate (K 2 CO 3 ) solution was performed using a wetted wall column at temperatures from 313 K to 343 K. Effect of arginine concentrations on absorption rates of CO 2 into carbonate solutions was investigated and the second-order rate constant k 1 was obtained using two-film model. Preliminary screening results showed that arginine was an effective promoter in aqueous K 2 CO 3 solution for CO 2 capture. The reaction of CO 2 with arginine is described using zwitterionic mechanism. The overall reaction kinetic constant k ov strongly increased with the increase of arginine concentration and absorption temperature under the investigated range. Based on the pseudo-first-order regime, the reaction rate parameters were obtained from the kinetic measurements and k 1 was determined as 33,641 and 85,367 L mol −1 s −1 at 315 K and 325 K, respectively with activation energy of 71.9 kJ mol −1 . The overall reaction rate for CO 2 absorption into promoted K 2 CO 3 were described as the following equation: - r CO 2 = 2.58 × 10 16 exp - 8645 T C Arg + 4.32 × 10 13 exp - 6666 T C OH - C CO 2 .
研究领域:
化学工程
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环境科学
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物理化学
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产品关联
DOI
高影响因子
POLYMER INTERNATIONAL
·
2022
·Vol. 71
·期 8
·1022-1029
·
IF 3.213
Mei Lin,Aizhu Lin,Sheng Huang,Tianhui Liu,Fang Ke,Deshun Qiu,Xuefeng Lin,Daoshu Luo
An ideal carrier that delivers small interfering RNA (siRNA) should be designed based on two criteria: high gene transfection efficiency and low cytotoxicity. In this work, arginine was used to modify polyvinylamine (PVAm) to form a novel PVAm derivative (PVAm-Arg) for siRNA delivery and the derivative was characterized using Fourier transform infrared spectroscopy. PVAm-Arg/siRNA complexes were formed by the self-assembly method. The zeta potential and size of the complexes were measured using electrophoretic light scattering and dynamic light scattering, respectively. The siRNA binding capacity of PVAm-Arg was assessed using gel retardation assay. The results showed that PVAm-Arg derivatives can bind siRNA effectively and form complexes with sizes of about 72 ± 2 nm. What is more, the ability of PVAm-Arg to condense siRNA was better than that of PVAm. Cytotoxicity assay with RSC96 cells also showed that PVAm-Arg had lower cytotoxicity compared with PVAm. In in vitro cellular uptake assay, PVAm-Arg showed good transfection efficiency. Therefore, we concluded that PVAm-Arg would be a promising candidate as a gene delivery vector. © 2022 Society of Industrial Chemistry.
研究领域:
生物技术
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药学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Yiwei Li,Yaohong Ma,Eric Lichtfouse,Jin Song,Rui Gong,Jinheng Zhang,Shuo Wang,Leilei Xiao
Para-Nitrophenol ( p -nitrophenol) is a common industrial pollutant occurring widely in water bodies, yet actual monitoring methods are limited. Herein we proposed a fully electrochemically in situ synthesized graphene-polyarginine composite functionalized screen printed electrode, as a novel p-nitrophenol sensing platform. The electrode was characterized by morphologic, spectrometric and electrochemical techniques. p -nitrophenol in both pure aqueous solution and real water samples was tested. Results show a detection limit as low as the nanomolar level, and display a linear response and high selectivity in the range of 0.5–1250 μM. Molecular simulation reveals a detailed synergy between graphene and poly-arginine. The preferable orientation of nitrophenol molecules on the graphene interface in the presence of poly-arginine induces H- and ionic binding. This sensor is an ideal prototype for p -nitrophenol quantification in real waters.
研究领域:
环境科学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2022
·
IF 5.112
Lili Lin,Saili Xie,Yingzheng Zhao,Zenghui Liang,Qilong Wu,Mingling Fang,Xiaolu Teng,Binbin Shi,Yunjun Yang,Bin Chen
Purpose Acute pancreatitis (AP) involves sudden inflammation caused by abnormal activation of pancreatic enzymes. The mechanisms underlying AP include oxidative stress, high levels of inflammatory mediators and inflammatory cell infiltration. Heparin, a key therapeutic drug, exerts anti-inflammatory, antioxidative, and anticoagulative effects. However, safe and effective drug delivery remains an obstacle. This study is the first to investigate the therapeutic effects of heparin-loaded microbubbles (HPMB) combined with ultrasound (UHPMB) and the role of heparin in acoustic cavitation. Methods The characteristics of the microbubbles, including particle size, concentration, release, stability, and development, were studied. Heparin concentration in the HPMB was measured, and heparin-induced anticoagulation was evaluated. Drug safety was explored using hemolysis and cell viability assessments. The ability of HPMB to alleviate oxidative stress and inflammation were investigated in vitro . L -arginine induces AP in vivo . UHPMB was used for AP treatment. Serum amylase levels were measured and pancreatic architecture and pathological features were evaluated to determine AP severity. In vivo efficacy was evaluated, and the underlying mechanism of heparin action during acoustic cavitation was explored. Results HPMB was spherical and presented as an emulsion-like solution without aggregation. HPMB was visible and stable and effectively released the drug under ultrasound (US). HPMB and UHPMB led to lower AP severity than in the untreated group. US-targeted microbubble destruction (UTMD) enhanced the therapeutic effect by decreasing oxidative stress and inflammation in AP models without injuring vital organs. UHPMB regulated VEGF/Flt-1 and SOD-1 expression. HPMB can also mitigate oxidative stress and inflammation in H 2 O 2 -pretreated cells. Conclusion UHPMB exhibits a strong ability not only to selectively target pancreatic lesions and release heparin but also to provide efficient protection by inhibiting oxidative stress and inflammation.
研究领域:
药理学
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治疗学
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医学成像
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炎症研究
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Zhuoran Yang,Chunyu Yang,Dan Yang,Ye Zhang,Qingzhu Yang,Fengyu Qu,Wei Guo
Ferroptosis has recently attracted much attention as an anti-tumor therapy. Evidence suggests that ferroptosis can induce oxidative stress and accumulation of lethal lipid peroxides in cancer cells, leading to cell damage. However, unsuitable pH, H 2 O 2 levels, and high glutathione (GSH) expression in the tumor microenvironment hinder the development of ferroptosis-mediated therapy. In this study, an l -arginine ( l -arg)-modified CoWO 4 /FeWO 4 (CFW) S-scheme heterojunction is strategically designed and constructed for ultrasound (US)-triggered sonodynamic- and gas therapy-induced ferroptosis. CFW not only has excellent Fenton-catalytic activity, outstanding GSH consumption capacity, and excellent ability to overcome tumor hypoxia, but its S-scheme heterostructure can also avoid the rapid combination of electron (e) and hole (h + ) pairs, thereby enhancing the sonodynamic effects. As a precursor of nitric oxide (NO), l -arg is modified on the surface of CFW (CFW@ l -arg) to achieve controlled NO release under US irradiation, thereby enhancing ferroptosis. In addition, poly(allylamine hydrochloride) is further modified on the surface of CFW@ l -arg to stabilize l -arg and achieve controllable NO release. Both in vitro and in vivo results demonstrate that such a multifunctional therapeutic nanoplatform can achieve high therapeutic efficacy through sonodynamic and gas therapy-enhanced ferroptosis. This designed oncotherapy nanoplatform provides new inspiration for ferroptosis-mediated therapy.
研究领域:
癌症治疗
·
纳米技术
·
材料科学
·
肿瘤学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Membranes
·
2023
·Vol. 13
·期 9
·760
·
IF 4.2
Yichen Chen,Wenmeng Yu,Hu Cao
Membrane fouling is an inevitable obstacle of polyamide composite forward osmosis (FO) membranes in oily wastewater treatment. In this study, zwitterionic arginine (Arg) is grafted onto nascent self-made FO polyamide poly(ether sulfone) (PA-PES) membrane, imparting superior hydrophilic, antifouling, and antibacterial properties to the membrane. Detailed characterizations revealed that the Arg-modified (Arg-PES) membrane presented obviously surface positively charged and unique morphology. Results showed that our strategy endowed the optimized membrane, the water flux increased by 113.2% compared to the pristine membrane, respectively, meanwhile keeping high NaCl rejection > 93.9% (with DI water as feed solution and 0.5 M NaCl as draw solution, FO mode). The dynamic fouling tests indicated that the Arg-PES membranes exhibited much improved antifouling performance towards oily wastewater treatment. The flux recovery ratios of the membrane were as high as 92.0% for cationic emulsified oil (cetyl pyridinium chloride, CPC), 87.0% for neutral emulsified oil (Tween-80), and 86.0% for anionic emulsified oil (sodium dodecyl sulfate, SDS) after washing, respectively. Meanwhile, the Arg-PES membranes assembled with guanidine cationic groups exhibited an enhanced antibacterial property againstE. coli, which exhibited a high antibacterial efficiency of approximately 96%. Consequently, the newly arginine functionalized FO membrane possesses impressive antifouling performance, while simultaneously resisting bacterial invasion, thus rendering it an ideal alternative for oily wastewater treatment in the FO process.Keywords:forward osmosis;chemical modification;wastewater treatment;antifouling;antibacterial
研究领域:
环境科学
·
膜技术
·
生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）