CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Qiuyan Zhang,Jun Tong,Wei Zhou,Zibiao Zhong,Qianchao Hu,Qiang Ma,Haitao Long,Shuangquan Wu,Xiaowen Shi,Qifa Ye
Hypothermic machine perfusion (HMP) is a preferable measure to preserve kidneys from donation after cardiac death (DCD), while the current standard perfusate is imperfect. We synthesized amphiphilic chitosan, N-alkylated-O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (N-alkyl-O-HTCC) as additive in the perfusate, which can self-assembly into micelles in water (size 133 ± 8.48 nm) (ζ-potential 43.9 ± 2.06 mV), with good antibacterial activity for Escherichia coli and Staphylococcus aureus . The derivates can also deliver hydrophobic drug Alda-1 into kidneys through HMP, with drug loading ratio (~42.14%). The delivery system specifically activated mitochondrial acetaldehyde dehydrogenase 2 in kidneys and reduced the ischemic injury. What's more, the addition of N-alkyl-O-HTCC in HMP also activated mitochondria superoxide dismutase 2, presented nice antioxidant activity . These all helped to improve the quality of DCD kidneys. This study provides a feasible amphiphilic carrier for hydrophobic drugs, and provides efficient guidance for perfusate improvement.
研究领域:
药学
·
生物医学工程
·
移植医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Yue Dong,Zihao Wei,Yuming Wang,Qingjuan Tang,Changhu Xue,Qingrong Huang
This work prepared food-grade oleogel-based Pickering emulsion stabilized by ovotransferrin (OVT)–carboxymethyl chitosan (CMCS) nanoparticles (OPEOC). The analysis of emulsified phase volumes and stability index exhibited that OPEOC with different volumes of oil fraction remained stable for 2 months of storage. The prepared Pickering emulsions were used as delivery systems for curcumin. In vitro digestive studies showed that OPEOC significantly improved the degree of lipolysis and the bioaccessibility of curcumin compared to the sunflower seed oil-based oleogel. The oleogel-based Pickering emulsions fabricated in the present study had noticeable storage stability, high loading and improved bioaccessibility of nutraceuticals, which might provide new insights into the development of novel food-grade Pickering emulsions with excellent properties.
研究领域:
食品科学
·
纳米技术
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2022
·Vol. 22
·期 7
·2100493
·
IF 5.859
Haishan Wu,Ting Guo,Jian Nan,Liu Yang,Guangfu Liao,Hyun Jin Park,Jinglei Li
Oral administration of insulin faces multiple biological challenges, such as varied digestive environments, mucin exclusion, and low epithelial cells’ absorption. In the present study, a hyaluronic acid (HA)-coated chitosan (CS) nanoparticle (HCP) delivery system is fabricated for insulin oral delivery. It is hypothesized that the developed nanoparticles will protect insulin from digestive degradation, promote intestinal epithelial cell absorption, and exert strong in vivo hyperglycemic ability. Nanoparticles formulated by CS and sodium tripolyphosphate (TPP) are optimized to form the core nanoparticles (CNPs). HA is further applied to coat CNP (HCP) to improve stability, reduce enzymatic degradation, and promote absorption of insulin. HCP promotes insulin uptake by Caco-2 cells, absorbs less mucin, and improves intestinal absorption. Moreover, an in vivo test demonstrates that oral administration of insulin-loaded HCP exerts strong and continuous hyperthermia effect (with a pharmacological availability (PA) of 13.8%). In summary, HCP is a promising delivery platform for insulin oral administration in terms of protecting insulin during digestion, facilitating its absorption and ultimately promoting its oral bioavailability.
研究领域:
药学
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纳米技术
·
药物递送系统
·
糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2022
·Vol. 6
·期 1
·1-14
·
IF 11.806
Xiao Lin,Xu Weichang,Huang Lixia,Liu Jili,Yang Guang
Effective postoperative therapy is crucial for preventing tumor recurrence and metastasis. Nanocomposite (NC) pastes of Gel/CS-g-CD NP with unique viscoelastic properties and pH-responsive drug delivery ability were prepared from gelatin (Gel) and β-cyclodextrin-grafted chitosan nanoparticles (CS-g-CD NPs) through host–guest interactions between the aromatic amino acid residues of gelatin and β-cyclodextrin. The dynamic rheological properties, shear strength, and shear viscosity were modulated through variations in the concentration of CS-g-CD NPs in the NC pastes. Due to the reversibility of the host–guest interactions, the NC pastes exhibited shear-thinning and self-healing properties, endowing them with good injectability. Loading of doxorubicin (DOX) in the nanoparticles (DOX-CSCD NPs) forming the NC pastes of Gel/DOX-CSCD NP allowed pH-triggered DOX release in a simulated tumoral microenvironment, because of the labile nature of the CS-g-CD NPs under acidic conditions. In vitro experiments showed that the NC pastes of Gel/DOX-CSCD NP induced sustained tumor cell inhibition. Moreover, in vivo studies with tumor-bearing mice indicated that the NC pastes of Gel/DOX-CSCD NP can effectively inhibit tumor recurrence after surgical resection. Therefore, the NC pastes described in this work may act as an effective injectable drug delivery system for postoperative tumoral treatment.
研究领域:
生物医学工程
·
药学
·
癌症研究
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
TRIBOLOGY INTERNATIONAL
·
2023
·
IF 6.2
Lumin Yang,Renjie Li,Xiaozhu Liao,Tao Li,Yunsong Kong,Xiaoduo Zhao,Rui Wang,Zhengfeng Ma,Zengjie Fan,Yong-min Liang,Shuanhong Ma,Feng Zhou
Osteoarthritis (OA) caused by the articular cartilage degeneration is closely related to the lubrication dysfunction of joint . At present, injecting biolubricants into the joint cavity is regarded as an effective strategy for the treatment of arthritis. Herein, a biocompatible chitosan (CS)-based nanoparticles (NPs) biolubricant ( [email protected] NPs) with dual function of lubrication and anti-inflammatory was fabricated. Zwitterionic polyelectrolyte brush poly(2-methacryloyloxyethyl phosphorylcholine, PMPC) was grafted onto the NPs via atom transfer radical polymerization (ATRP) strategy, while the anti-inflammatory drug (diclofenac sodium, DS) was encapsulated within them. Under the synergistic effect rolling lubrication and hydration lubrication mechanisms, the obtained biolubricant exhibited superior lubrication performance in either artificial joint materials or cartilage contacts, which presents great potential for OA treatment.
研究领域:
生物医学工程
·
材料科学
·
骨科
·
药学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Haishan Wu,Jingbo He,Haoran Cheng,Liu Yang,Hyun Jin Park,Jinglei Li
Chitosan-based nanoparticles (CNPs) are widely used in drug delivery, cosmetics formulation and food applications. To accelerate the manufacturing of CNPs, the present study develops a workflow to prepare CNPs in a continuous model. Based on machine learning, the workflow precisely predicts size and polymer dispersity index (PDI) value of CNPs, which impacts on the colloidal stability and applications. Multi-inlet vortex mixer (MIVM) device was fabricated by 3D printing as the reactor. Peristaltic pump was applied to deliver the reaction streams into the MIVM device and produce CNPs by flash nanoprecipitation (FNP) in a continuous way. The developed MIVM device produces CNPs in a controlled manner at a higher output which is promising for upscale applications. Twelve machine learning algorithms were employed to investigate the potential relationship between the reaction independent variables and hydrodynamic characteristics of CNPs. Random Forest, Decision Tree, Extra Tree and Bagging algorithms performed better than other algorithms with the average prediction accuracy around 90 %. The current study demonstrated that supervised machine learning guided FNP using the developed MIVM device is an effective strategy for accurate and intelligent production of CNPs and other similar nanoparticles.
研究领域:
生物工程
·
纳米技术
·
制药工程
✓ 已验证
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2013
·Vol. 9
·期 5
·863-869
·
IF 5.256
Zhang Li,Liu Yongjun,Yu Dexin,Zhang Na
The aim of our study was to prepare gadolinium loaded chitosan nanoparticles (Gd-CSNPs) for magnetic resonance imaging (MRI). The chitosan nanoparticles (CSNPs) were prepared by ionic gelation method with sodium tripolyphosphate. The Gd ions were conjugated to the surface of CSNPs through
diethylenetriamine pentaacetic acid (DTPA) using N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to obtain Gd-CSNPs. The physicochemical properties of CSNPs and Gd-CSNPs were measured by transmission electron microscope,
dynamic light scattering and inductively coupled plasma optical emission spectroscopy, respectively. The cell toxicity evaluation was performed in mouse B16 cells by MTT assay. The T1-weighed magnetic resonance images were measured by a 3.0 T Sigma scanner. The morphologies of the CSNPs and
Gd-CSNPs were spherical or ellipsoidal in shape. The mean sizes of the CSNPs and Gd-CSNPs were 110.9 ± 6.8 nm and 153.0 ± 7.5 nm, respectively. The zeta potentials of the CSNPs and Gd-CSNPs were 22.30 ± 0.77 mV and 13.91 ± 4.26 mV, respectively. The relaxation rates
of Gd-CSNPs and Magnevist
®
were 7.509 mM–1 · s–1 and 3.052 mM–1 · s–1, respectively. The Gd-CSNPs exhibited high T1 relaxivity and no obvious cytotoxicity was observed under the experimental
concentrations in mouse B16 melanoma cells. These results indicated that the Gd-CSNPs had great potential as MRI contrast agents for the early diagnosis of tumor.
研究领域:
医学影像
·
纳米技术
·
药学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 5
·1635
·
IF 4.556
Yanan Wang,Shangyong Li,Mengfei Jin,Qi Han,Songshen Liu,Xuehong Chen,Yantao Han
The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. Lysozyme degrades bacterial cell wall without involving antibiotic resistance and has become a new antibacterial strategy. However, direct use of native, active proteins in clinical settings is not practical as it is fragile under various conditions. In this study, lysozyme was integrated into chitosan nanoparticles (CS-NPs) by the ionic gelation technique to obtain lysozyme immobilized chitosan nanoparticles (Lys-CS-NPs) and then characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM), which showed a small particle size (243.1 ± 2.1 nm) and positive zeta potential (22.8 ± 0.2 mV). The immobilization significantly enhanced the thermal stability and reusability of lysozyme. In addition, compared with free lysozyme, Lys-CS-NPs exhibited superb antibacterial properties according to the results of killing kinetics in vitro and measurement of the minimum inhibitory concentration (MIC) of CS-NPs and Lys-CS-NPs againstPseudomonas aeruginosa(P. aeruginosa),Klebsiella pneumoniae(K. pneumoniae),Escherichia coli(E. coli), andStaphylococcus aureus(S. aureus). These results suggest that the integration of lysozyme into CS-NPs will create opportunities for the further potential applications of lysozyme as an anti-bacterium agent.Keywords:immobilization;chitosan nanoparticles;lysozyme;anti-bacterium activity
研究领域:
药学
·
纳米技术
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Li Lin,Junyi He,Jiawei Li,Yan Xu,Jingxia Li,Yangzhe Wu
Background During the past few years, immune cell therapy for malignant cancer has benefited a considerable amount of patients worldwide. As one of several promising candidates for immunotherapy, Vγ9Vδ2 γδ T cells have many unique biological advantages, such as non-MHC restriction and have been noted as the earliest source of IFN-γ. However, potentiating anti-tumor functions of γδ T cells has become of particular interest to researchers studying γδ T cell applications.Purpose In this study, we proposed a nanotechnology-based methodology for strengthening γδ T cell functions.Methods As a type of reliable, biocompatible material, chitosan nanoparticles (CSNPs) were used to enhance anti-tumor immunity of γδ T cells.Results First, we found that the size of prepared CSNPs distributed 50 to 100 nm, and that CSNPs had optimal immunocompatibility. Then, we observed that CSNPs could induce α-tubulin cytoskeleton polarization and rearrangement, correlating with a higher killing ability of γδ T cells. Furthermore, we revealed that CSNPs could enhance Vγ9Vδ2 T cell anti-tumor functions by upregulating killing of related receptors, including NKG2D, CD56, FasL, and perforin secretion.Conclusion Our work provided evidence of application for CSNPs based bio-carrier in immunotherapy. More importantly, we proposed a new strategy for enhancing γδ T cell anti-tumor activity using nanobiomaterial, which could benefit future clinical applications of γδ T cells.
研究领域:
免疫学
·
纳米技术
·
肿瘤学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ruixin Li,Jinxia He,Hongguo Xie,Wenxia Wang,Santosh Kumar Bose,Yeqing Sun,Jianen Hu,Heng Yin
To investigate the effect and mechanism of chitosan nanoparticles (CSNPs) on the germination and seedling growth of wheat ( Triticum aestivum L.), we conducted systematic research on the impact of different concentrations (1–100 μg/mL) of CSNPs and chitosan (CS). The result of energy-dispersive spectroscopy (EDS) and confocal laser scanning microscopy (CLSM) showed that adsorption of CSNPs on the surface of wheat seeds was higher than that of CS. CSNPs had growth promoting effect at a lower concentration (5 μg/mL) compared with CS (50 μg/mL). In addition, the application of 5 μg/mL CSNPs induced the auxin-related gene expression, accelerated indole-3-acetic acid (IAA) biosynthesis and transport, and reduced IAA oxidase activity resulting in the increase of IAA concentration in wheat shoots and roots. The results suggest that CSNPs have positive effect on seed germination and seedling growth of wheat at a lower concentration than CS due to higher adsorption on the surface of wheat seeds.
研究领域:
植物学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Qing Wan,Meiying Liu,Dazhuang Xu,Liucheng Mao,Jianwen Tian,Hongye Huang,Peng Gao,Fengjie Deng,Xiaoyong Zhang,Yen Wei
Chitosan based nanomaterials have been extensively examined for biomedical applications for their biodegradability, low toxicity, biological activity and low cost. In this work, a novel strategy for fabrication of luminescent polymeric nanoparticles (LPNs) based on aggregation induced emission (AIE) dye and water soluble chitosan (WS-Chitosan) were firstly developed via a highly efficient mercaptoacetic acid (MA) locking imine reaction. In this multicomponent reaction (MCR), MA serves as “lock” to connect 9,10-Bis(aldehydephenl)anthracene dye (An-CHO) and amino-containing WS-Chitosan under mild reaction conditions. The obtained WS-Chitosan@An-CHO LPNs show strong yellow emission and great water dispersibility. Biological evaluation results demonstrated that synthetic luminescent polymeric nanoparticles possess desirable cytocompatibility and distinct imaging properties. Therefore, we have developed a facile and useful method to fabricate AIE active nanoprobes with desirable properties for various biomedical applications. This strategy should be a general and easy handling tool to fabricate many other AIE dye based materials.
研究领域:
材料科学
·
生物医学工程
·
高分子化学
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Chao Luo,Cong Chen,Na Zhang,Jiani Li,Yaru Liang,Weibin Wu,Kai Yang
Tanshinone IIA, an important active ingredient isolated from Salvia miltiorrhiza , exhibits a broad spectrum of biological activities including anti-inflammatory, anti-oxidative, and anti-tumor. However, its poor water solubility results in low bioavailability and limits the subsequent clinical application. Chitosan nanoparticle is an excellent drug delivery carrier due to the ideal biocompatibility and pH-sensitive. Here, we developed an innovative and efficient approach to fabricate the tanshinone IIA-chitosan nanoparticles (TA/CS-NPs) by initially creating a tanshinone IIA-chitosan solid dispersion as the precursor for nanoparticle synthesis. This method successfully overcame the problem that the hydrophobic tanshinone IIA could not be directly mixed with the hydrophilic chitosan to prepare nanoparticles. The optimal conditions for TA/CS-NPs preparation were obtained through the response surface method, with the chitosan concentration of 31 mg/ml, the mass ratio of CS to TA 27:1, and stirring time 30 min. In vitro release and cell uptake experiments proved that the TA/CS-NPs had pH-sensitive drug release property. In ph5.0 dissolution medium or the medium of inflammatory cell, the nanoparticles revealed rapid drug release properties. However, in the ph7.4 dissolution medium or the medium of normal cells, the drug release rate of nanoparticles was very slow. Subsequently, cellular experiments demonstrated that the anti-inflammatory and antioxidant activities of tanshinone IIA were markedly enhanced through nanoparticle delivery. Compared with direct administration of tanshinone IIA, TA/CS-NPs have more significant activity on the inhibition of inflammatory factors (IL-1β, IL-6, TNF-α and NO), regulation of oxidative stress products (ROS, MDA) and antioxidant products (SOD, GSH, and CAT), protective effect on mitochondrial damage, inhibition of inflammatory signaling pathways (p-p65, NLRP3). This study provides a successful method to encapsulate hydrophobic drugs into hydrophilic chitosan nanoparticles and improve the therapeutic activities of the loaded drug.
研究领域:
药剂学
·
生物医学工程
·
中药学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）