INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Zhihui Chai,Chenglin Li,Yuan Zhu,Xiaorong Song,Manyi Chen,YuLing Yang,Dong Chen,Xinquan Liang,Junhua Wu
The presence of phenolic substances in sugarcane juice seriously affects the color value of sugar products. Magnetic chitosan (MCS) was prepared using an ionic cross-linking technique, and then was modified with arginine to prepare arginine-modified magnetic chitosan (AMCS) for use as a new sucrose clarifying adsorbent. Gallic acid (GA) is a representative phenolic substance and was used to test the adsorption properties of the prepared AMCS. The adsorption kinetics indicated that the adsorption of GA on AMCS conformed to the pseudo-second-order model, the main adsorption mechanism was chemisorption. The Langmuir equation fit well and with good linearity, and indicated a maximum adsorption capacity of 48.38 mg g −1 . The adsorption process was consistent with monolayer adsorption and spontaneous endothermic process. The prepared AMCS exhibited excellent regenerability, and can effectively remove GA in sugarcane juice to improve product safety.
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Food Packaging and Shelf Life
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2023
·
IF 8
Pingping Tang,Tingting Zheng,Ruimin Ran,Yongming Xiong,Guoying Li
Collagen films were functionalized with different concentrations of gallic acid (GA) in the presence of laccase. FTIR results suggested that crosslinking bonds formed between the amino groups of collagen and quinone radicals generated from the oxidized phenolic hydroxyl groups of GA by laccase. The amount of cross-linking gradually increased with increasing GA concentration. The formed covalent cross-linking bonds strengthened the combination between collagen and GA, improving the stability of GA in the film matrix. The tensile strength and denaturation temperature of collagen film was increased by 43.01 MPa and 17.9 °C, respectively, at GA concentration of 8 mmol. When GA concentration was ≥ 4 mmol, the films exhibited obvious scavenging activity against DPPH free radicals and antibacterial activities against S. aureus and E. coli . Film with 8 mmol GA could reduce the thiobarbituric acid reactive substance values, total viable count and total volatile basic nitrogen content of beef during storage, showing a good application potential in food packaging.
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mBio
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2023
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IF 6.4
Jie Peng,Tong Liu,Pengfei Meng,Yue Luo,Siyue Zhu,Yanxin Wang,Mingxia Ma,Jiaojiao Han,Jun Zhou,Xiurong Su,Shiming Li,Chi-Tang Ho,Chenyang Lu
Gut microbiota dysbiosis is causally related to inflammatory bowel disease (IBD), and increased levels of the gut metabolite ammonia have been proposed to contribute to IBD development. In this study, we aimed to clarify the anti-colitis mechanism of gallic acid (GA) based on its ability to trap the deleterious metabolite ammonia and improve gut microbiota. Aminated product was detected in the fecal samples of mice after oral gavage of gallic acid (GA) and identified as 4-amino-substituted gallic acid (4-NH2-GA), thus confirming the ability of GA to trap ammonia in vivo. Then, we compared the beneficial effects of GA and 4-NH2-GA on dextran sulfate sodium (DSS)-induced colitis mouse and found that both compounds managed to alleviate colitis phenotypes, indicating ammonia trapping had no adverse effect on the original anti-colitis activity of GA. In addition, both GA and 4-NH2-GA improved the gut microbiota dysbiosis induced by DSS, and fecal microbiota transplantation was subsequently performed, which further revealed that the gut microbiota mediated the anti-colitis activity of both GA and 4-NH2-GA. In summary, this study clarified that GA alleviated colitis by targeting both the symptoms and root causes: it directly reduced the deleterious metabolite ammonia by forming aminated metabolites without compromising the original anti-colitis activity, and it also improved gut microbiota dysbiosis, which in turn contributed to the alleviation of colitis. Since the GA structure is presented in various polyphenols as a common building block, the novel anti-colitis mechanism obtained from GA may also apply to other complex polyphenols.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-18
·
IF 16.6
Li Guoqing,Liu Su,Chen Yixiao,Zhao Jin,Xu Huihui,Weng Jian,Yu Fei,Xiong Ao,Udduttula Anjaneyulu,Wang Deli,Liu Peng,Chen Yingqi,Zeng Hui
Intra-articular injection of therapeutics is an effective strategy for treating osteoarthritis (OA), but it is hindered by rapid drug diffusion, thereby necessitating high-frequency injections. Hence, the development of a biofunctional hydrogel for improved delivery is required. In this study, we introduce a liposome-anchored teriparatide (PTH (1–34)) incorporated into a gallic acid-grafted gelatin injectable hydrogel (GLP hydrogel). We show that the GLP hydrogel can form in situ and without affecting knee motion after intra-articular injection in mice. We demonstrate controlled, sustained release of PTH (1–34) from the GLP hydrogel. We find that the GLP hydrogel promotes ATDC5 cell proliferation and protects the IL-1β-induced ATDC5 cells from further OA progression by regulating the PI3K/AKT signaling pathway. Further, we show that intra-articular injection of hydrogels into an OA-induced mouse model promotes glycosaminoglycans synthesis and protects the cartilage from degradation, supporting the potential of this biomaterial for OA treatment.
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CHEMICAL ENGINEERING JOURNAL
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2019
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IF 8.355
Tong-liang Wu,Qian Sun,Guo-dong Fang,Pei-xin Cui,Cun Liu,Marcelo Eduardo Alves,Wen-xiu Qin,Dong-mei Zhou,Zhen-qing Shi,Yu-jun Wang
Natural organic matter (NOM) profoundly influences the metal(loid)s immobilization by iron (Fe) minerals, which, in turn, are the host phase for Sb that control its mobilization and bioavailability. However, the potential effect of the redox-active phenolic acids derived from NOM on this process has rarely been explored. Therefore, the adsorption of Sb(III) on goethite and Sb(III) oxidation in the presence of a common phenolic acid—gallic acid (GA)—was systematically investigated. The reaction mechanisms were unraveled by the combination of wet chemistry, electron paramagnetic resonance (EPR), X-ray photoelectron spectroscopy (XPS), and X-ray absorption fine structure (XAFS) spectroscopy. Our results showed the addition of GA significantly inhibited Sb adsorption on goethite as the pH increased. Except for the competitive adsorption by GA, the inhibition also resulted from the extensive Sb(III) oxidation in the suspension and the lower Sb(V) sorption capacity of goethite at higher pH. The alkaline condition facilitated the generation of reactive oxygen species (hydrogen peroxide) and quinone species (semiquinone radical (SQ − ) and oxidized GA), accounting for the significant Sb(III) oxidation in the solution and on the goethite surface. Besides, the edge-sharing inner-sphere complexes were formed between Sb and goethite regardless of the presence of GA. Our findings demonstrated the remarkable role of the phenolic groups in NOM on the adsorption of Sb(III) on goethite.
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Biomaterials Science
·
2019
·Vol. 7
·期 8
·3258-3265
·
IF 5.251
Shuting Lu,Liping Liu,Henan Wang,Wancheng Zhao,Zeyu Li,Zheng Qu,Jialu Li,Tiedong Sun,Ting Wang,Guangchao Sui
Carbon quantum dots are excellent photoluminescent materials because of their unique fluorescence properties. They are widely used in biomedical imaging due to their good biocompatibility. However, carbon quantum dots with antitumor activity have rarely been reported. Gallic acid (GA) is an anticancer agent and effective against many types of tumor cells. In this study, GA based carbon dots (GACDs) with fluorescence and antitumor activity were synthesized by a simple microwave-assisted method and characterized by transmission electron microscopy (TEM), and Fourier transformed infrared (FTIR) and X-ray photoelectron spectroscopy (XPS). Studies of optical properties indicated that the GACDs exhibited significant photoluminescence. In addition, we observed the antitumor activity of the GACDs using both cell-based assays and mouse xenograft tumors. Our results demonstrated that the GACDs can be used as both a bioimaging material and an antitumor agent, suggesting their great potential in future clinical applications.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Chang Liu,Pan-Pan Tang,Xiu-Bin Liu,Jun-Xiang Liu,Xiao-Hong Pan,Rana Muhammad Aadil,Jun-Hu Cheng,Zhi-Wei Liu
The utilization of cold plasma (CP) treatment to promote covalent conjugation of ovalbumin (OVA) and gallic acid (GA), as well as its functionality, were investigated. Results demonstrated that CP significantly enhanced the covalent grafting of OVA and GA. The maximum conjugation of GA, 24.33 ± 2.24 mg/g, was achieved following 45 s of CP treatment. Covalent conjugation between GA and OVA were confirmed through analyses of total sulfhydryl (-SH) group, Fourier transform infrared (FTIR) spectroscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Unfolding of the OVA molecule occurred upon conjugation with GA, as evidenced by multiple spectroscopy analyses. Additionally, conjugation with GA resulted in significant improvements in the antioxidant activity and emulsifying properties of OVA. This study demonstrated that CP is a robust and sustainable technique for promoting the covalent conjugate of polyphenols and proteins, offering a novel approach to enhance the functional properties of proteins.
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International Journal of Nanomedicine
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2024
·
IF 6.6
Nanan Miao,Tao Jiang,Yuanchao Li,Sihong Xue,Shilei Hao,Chunli Zhou,Yujie Gu,Ran Li,Bo Yu,Xiaoqu Duan,Wenchao Xu,Rupeng Wang,Lei Ran
Background Wound healing is a complex physiological process that can be roughly divided into four stages: hemostasis, inflammation, proliferation, and remodeling. Conventional wound dressings often fail to meet the diverse needs of these healing stages due to their limited functionality. Cryogels, however, possess several attractive properties, such as large, interconnected pores, good mechanical strength, and ease of modification, making them suitable for developing advanced dressings with multiple functions. In this study, we developed a multifunctional cryogel dressing, with biocompatible polysaccharides as the main component, designed to provide a breathable, moist, and antibacterial microenvironment for chronic infected wounds, thereby promoting wound healing.Methods Recombinant keratin 31 (RK31) was combined with chitosan (CS) to produce a CS/RK31 cryogel, referred to as CK. Gallic acid-reduced silver nanoparticles (GA/Ag NPs) were incorporated as the active antibacterial component to create the CS/K31@GA/Ag cryogel, known as CKGA. The cryogel was characterized using scanning electron microscopy (SEM) and a universal testing machine, and its biocompatibility was assessed in vitro. The dynamic hemostatic performance of the cryogel was evaluated with a rat tail amputation bleeding model. Additionally, the antibacterial effects of the cryogel against Staphylococcus aureus and Escherichia coli were tested using agar diffusion assays and turbidimetry. The antioxidant capacity of the CKGA cryogel was also measured in vitro. Finally, the cryogel’s ability to promote wound healing was tested in an SD rat model of infected wounds.Results Characterization results showed that the CKGA cryogel features an interpenetrating porous network structure and exhibits excellent mechanical properties, with a swelling rate of up to 1800%. Both in vitro and in vivo experiments confirmed that the cryogel has good biocompatibility, effectively absorbs exudates, and rapidly stops bleeding. The addition of GA/Ag NPs provided significant antibacterial effects, achieving an inhibition rate of over 99.9% against both S. aureus and E. coli. Furthermore, CKGA cryogels demonstrated a strong scavenging capacity for ROS in a dose-dependent manner. Studies using the SD rat infected wound model showed that the cryogel effectively inhibited bacterial proliferation on wound surfaces, reduced local tissue inflammation, and promoted the healing of infected wounds.Conclusion The multifunctional cryogel, with its rapid hemostatic, antibacterial, and antioxidant properties, as well as its ability to promote cell proliferation, could be widely used as a wound dressing for the healing of bacterial infections.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Qianwei Qu,Wenqiang Cui,Xingyu Huang,Zhenxin Zhu,Yue Dong,Zhongwei Yuan,Chunliu Dong,Yadan Zheng,Xueying Chen,Shuguang Yuan,Yanhua Li
Due to the large amount of antibiotics used for human therapy, agriculture, and even aquaculture, the emergence of multidrug-resistant Streptococcus suis (S. suis) led to serious public health threats. Antibiotic-assisted strategies have emerged as a promising approach to alleviate this crisis. Here, the polyphenolic compound gallic acid was found to enhance sulfonamides against multidrug-resistant S. suis. Mechanistic analysis revealed that gallic acid effectively disrupts the integrity and function of the cytoplasmic membrane by dissipating the proton motive force of bacteria. Moreover, we found that gallic acid regulates the expression of dihydrofolate reductase, which in turn inhibits tetrahydrofolate synthesis. As a result of polypharmacology, gallic acid can fully restore sulfadiazine sodium activity in the animal infection model without any drug resistances. Our findings provide an insightful view into the threats of antibiotic resistance. It could become a promising strategy to resolve this crisis.
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CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Liyun Rui,Minhao Xie,Bing Hu,Li Zhou,Danyang Yin,Xiaoxiong Zeng
Gallic acid-grafted-chitosan (GA- g -CS) was synthesized in the presence of 1-ethyl-3-(3′-dimethylaminopropyl) carbodiimide and hydroxybenzotriazole under room temperature. To develop a kind of newly functional film, CS, gelatin, glycerol and Tween 20 were utilized to prepare CS-based composite films with incorporated GA (GA-CS) or conjugated GA (GA- g -CS), and the films were characterized in aspects of physical and functional properties, as well as microstructures. With the increasing of GA concentration, there was a reduction in transmittance, elongation at break and water vapor permeability (WVP) for GA-CS film. For GA-CS films, the higher tensile strength and lower WVP could be obtained when GA was evenly distributed across the films. Different from the GA-CS films, water solubility, swelling ratio, water vapor permeability and scavenging activities on 2,2-Diphenyl-1-picrylhydrazyl/2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) radicals were enhanced for GA- g -CS films, which could be ascribed to the rough surface morphology. The GA- g -CS films exhibited enhanced water vapor permeability when the substitution degree of GA increased. Also, the antimicrobial activities of GA- g -CS films were superior to those of GA-CS films. Our present work developed a way to prepare GA- g -CS films that owned many desirable properties and could be explored as promising biomaterials in food packaging.
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NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Juan Zhou,Ying Huang,Yisen Wang,Junfeng Zhu,Shaohui Cheng,Juqun Xi,Hua Yan
Skin wound infections pose significant challenges in clinical practice, often leading to delayed healing and systemic complications. The overuse of antibiotics has exacerbated bacterial resistance, necessitating the development of alternative antibacterial strategies. Inspired by natural small molecule self-assembly, we developed a novel antibacterial dressing by inducing gallic acid (GA) to self-assemble into fibers through coordination with manganese ions (Mn2+). The Mn2+/GA self-assembled fibers exhibited excellent biocompatibility, sustained GA release, and enhanced antibacterial activity. In vitro and in vivo studies demonstrated that these fibers significantly promoted the healing of Staphylococcus aureus-infected wounds by inhibiting bacterial growth and reducing inflammation. This study provides a new therapeutic strategy for treating infected wounds using metal ion-induced self-assembly of natural drug molecules.
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IUBMB LIFE
·
2020
·Vol. 73
·期 2
·398-407
·
IF 3.244
Zhou Jing,Minghe Li,Hongyuan Wang,Zhuo Yang,Shaolong Zhou,Jian Ma,Enping Meng,Hengwei Zhang,Wulong Liang,Weihua Hu,Xinjun Wang,Xudong Fu
Glioblastoma multiforme (GBM) is among the most common adult brain tumors with invariably fatal character. Following the limited conventional therapies, almost all patients, however, presented with symptoms at the time of recurrence. It is dire to develop novel therapeutic strategies to improve the current treatment of GBM. Gallic acid is a well-established antioxidant, presenting a promising new selective anti-cancer drug, while gold nanoparticles (GNPs) can be developed as versatile nontoxic carriers for anti-cancer drug delivery. Here, we prepared gallic acid-GNPs (GA-GNPs) by loading gallic acid onto GNPs, reduction products of tetrachloroauric acid by sodium citrate, through physical and agitation adsorption. GA-GNPs, rather than GNPs alone, significantly inhibited the survival of U251 GBM cells, as well as enhanced radiation-induced cell death. Moreover, GA-GNPs plus radiation arrested the cell cycle of U251 at the S and G2/M phases and triggered apoptotic cell death, which is supported by increased BAX protein levels and decreased expression of BCL-2. Thus, GA-GNPs have great potential in the combination with radiation therapy in future studies for GBM treatment.
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