International Journal of Nanomedicine
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2026
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IF 8.7
Mengqi Wang,Mengxuan Jia,Rongrong Zhang,Caixia Xue,Xue Ma,Mingjun Song,Gang Liu
Background: The theranostic potential of glycine-derived carbon dots (Gly/CDs) against arsenic-induced hepatotoxicity remains largely unexplored. This study aimed to synthesize Gly/CDs via a green, microwave-assisted method and to systematically evaluate their hepatoprotective efficacy and underlying mechanisms in a sodium arsenite (NaAsO2)-induced hepatic injury model.Methods: Gly/CDs were synthesized using citric acid, urea, and glycine as precursors. Their physicochemical properties were characterized using transmission electron microscopy (TEM), fluorescence spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and X-ray photoelectron spectroscopy (XPS). Following in vivo biocompatibility assessment, a murine model of NaAsO2-induced hepatotoxicity was established to evaluate therapeutic efficacy. Comprehensive biochemical assays, histopathological examinations, and transcriptomic analyses were conducted to assess oxidative stress, inflammatory responses, DNA damage, apoptosis, fibrosis, and the involved molecular pathways.Results: Gly/CDs exhibited excellent biocompatibility and demonstrated significant hepatoprotective effects, including restoration of redox homeostasis, suppression of pro-inflammatory cytokines, and attenuation of hepatocellular DNA damage, apoptosis, and fibrotic remodeling. Transcriptomic profiling suggested the involvement of the PI3K/AKT signaling pathway as a key molecular axis associated with the observed therapeutic effects. Overall, Gly/CDs preserved hepatic structure and function under chronic arsenic exposure.Conclusion: This study provides the first comprehensive evidence that Gly/CDs function as biologically active nano-antioxidants capable of mitigating arsenic-induced hepatotoxicity through redox modulation and modulation of PI3K/AKT signaling activity. Given their low toxicity, ease of synthesis, and multifunctional properties, Gly/CDs represent a promising nanotherapeutic platform for applications in redox biology, toxicology, and environmental health.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2023
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IF 6
Hui Tang,Yun Li,Wancheng Bao,Pengfei Wang,Xiaomeng Wang,Qinqin Su,Yusheng Zhao,Jinlong Zhu,Songbai Han
Understanding the effects of clay minerals and amino acids on methane hydrate (MH) formation is essential for elucidating the occurrence of MH in marine sediments. In this study, kinetic experiments and molecular dynamics simulations of MH formation in montmorillonite and kaolinite systems with the presence of glycine were investigated. The experimental results showed that the addition of 5 wt% montmorillonite and 5 wt% kaolinite inhibited and promoted MH formation, respectively. Adding 1.5 wt% glycine to the montmorillonite suspension shortened the nucleation time and reduced the amount of MH; it also weakened the facilitating effect of the kaolinite suspension. The simulation results showed MH nucleation in the bulk solution due to the hydration properties of Ca 2+ on the external surface of montmorillonite. However, the addition of glycine reduced the inhibitory effect of Ca 2+ on MH formation due to the electrostatic interaction between the Ca 2+ and COO – groups. Adsorbed methane molecules on the kaolinite siloxane surface formed clathrate-like structures. In addition, glycine also abnormally incorporated into the MH structure to inhibit MH nucleation. These findings imply that the clay mineral had a significant effect on the distribution of amino acids, causing the formation of MH in clay mineral suspension to be different from pure water. Therefore, these interactions should be fully considered when studying the occurrence mechanism of natural gas hydrate when clay minerals and amino acids coexist in marine sediments.
研究领域:
能源地质学
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物理化学
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海洋沉积学
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分子模拟
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RSC Advances
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2018
·Vol. 8
·期 37
·20719-20725
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IF 2.936
Yan Gao,Yunhong Zhang
Increasing attention has been paid to atmospheric “brown carbon” (BrC) aerosols due to their effect on the earth's climate. Aqueous BrC aerosols were formed through aqueous reactions of hydroxyacetone (HA) with nitrogen compounds such as glycine (Gly) and/or ammonium sulfate (AS). When exposed to nitrogen compounds for several days, HA, as a type of aqueous carbonyl compound, becomes absorbent and fluorescent in the blue visible and near ultraviolet regions, which have been monitored by UV/vis and fluorescence spectroscopy. In this study, we quantified absorption and excitation-emission matrix (EEM) spectra in the formation of aqueous BrCs, which was generated from HA-Gly and HA-Gly-AS mixtures, respectively. The obtained data was used to determine the base-10 absorption coefficient (α), absorption Angstrom exponent (AAE), and effective quantum yield (QY). All of the related parameters provide further evidence for the formation of aqueous BrC. The absorbances of the as-obtained BrCs follow the order HA-Gly-AS > HA-Gly > HA-AS. In other words, HA-Gly-AS mixtures displayed the most intense absorbances, whereas HA-AS mixtures barely produced visible absorbance. It is reasonable to speculate that Gly promotes the formation of HA-Gly BrC mixtures. The experimental results are consistent with previous measurements reported by Powelson et al. BrCs from HA-Gly-AS and HA-Gly exhibit stronger fluorescence between 300 and 400 nm. Glycine plays a more important role in the formation of aqueous BrC than that of AS. Furthermore, we examined the mass absorption coefficient (MAC) by photolysis of aqueous BrCs, which resulted from the oxidation of HA-Gly and HA-Gly-AS mixtures by 5 mM H2O2. An effective photolysis time induced significant changes near-UV (300–400 nm) absorption intensity of HA-Gly and HA-Gly-AS mixtures. These results emphasize the dynamic nature of the corresponding atmospheric aqueous BrC. Overall, our study provides the optical properties of the corresponding atmospheric aqueous BrC and the H2O2 oxidation photolysis process of the as-obtained BrC in detail, which may contribute to the understanding of the important effects of aqueous BrC for atmospheric chemistry and climate.
研究领域:
大气化学
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环境科学
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气溶胶物理
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光化学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2026
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IF 13.2
Wangyang Mei,Lili Li,Zhaoxia Wang,Qingwei Gao,Xinwen Li,Liangliang Wei,Guangzhi Wang,Junqiu Jiang,Qingliang Zhao
Glycine betaine (GB) has been used to resist different environmental stress. However, its effects and mechanisms in mitigating salt stress to recover methane yield in anaerobic digestion (AD) remains unclear. This study focused on investigating the alleviation efficiency and mechanism of salt stress in AD of typical saline organic wastes by GB. GB could facilitate hydrolysis, acidogenesis and methanogenesis steps, resulting in a methane yield increase of 156.3 mL·g −1 VS under 10 g·L −1 NaCl. Additionally, GB stimulated extracellular polymeric substances (EPS) secretion by 23% and modulated key enzyme activities (α-GC, F420 and TAOC increased; NP, TBARS, SOD and CAT decreased), thereon restoring metabolic and redox balance. Microbial analysis indicated that GB tightened the microbial network, especially the co-relationship between Methanothrix and other microbes. Metagenomics further revealed upregulation of genes related to methanogenesis (e.g., ftr , mtd and mer ), osmoregulation (e.g., proV , kdpA and envZ / ompR ), and EPS secretion (e.g., bapA , rfbA and tatA ). Furthermore, GB directly participated in methanogenesis pathways. This study not only proposes a broad-spectrum approach to mitigate salt stress of salt-containing AD, but also establishes a universal theoretical framework for designing resilient AD systems from a microbial perspective.
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高影响因子
AMINO ACIDS
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2023
·Vol. 55
·期 3
·385-402
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IF 3.5
Wang Huan,Ni Xiaoyu,Dong Weichong,Qin Weiman,Xu Lei,Jiang Ye
We developed a hollow fiber centrifugal ultrafiltration (HFCF-UF) method to study the change of plasma levels of free glycine (Gly) in patients with acute ischemic stroke (AIS). Twenty-four patients with AIS confirmed by diffusion-weighted imaging (DWI) were enrolled. During the study period, the patients did not receive any supplemental amino acids therapy that could affect the obtained results. Our results showed that although AIS patients adopted different methods of treatment (thrombolytic and non-thrombolytic), the clinical NIHSS score of AIS showed a downward trend whereas Gly concentration showed increased trend. Moreover, plasma free Gly concentration was positively correlated with ASPECTS score. The correlation between Gly levels and infarct volume showed a statistical significance. That is to say, higher Gly level predicted smaller infarct size. Thus, the change of free Gly level in plasma could be considered as a potential biomarker of AIS.
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MINERALS ENGINEERING
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2023
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IF 4.8
Lei Hou,Alejandro López Valdivieso,Peng Chen,Guowei Zhang,Qi Zhang,Yu Chen,Shaoxian Song,Feifei Jia
Ammonia-free thiosulfate systems are known for their non-toxicity, low reagent consumption, and excellent leaching efficiency in gold extraction studies. This study proposes a novel and environmentally friendly glycine-thiosulfate system and comprehensively investigates the dissolution behavior of gold through the application of electrochemical methods. Electrochemical quartz crystal microbalance (EQCM) has demonstrated that the anodic dissolution of gold is predominantly influenced by the forms of copper(II)-glycine complexes as catalysts, and the presence of Cu(C 2 H 4 NO 2 ) 3 − complex in strong alkaline has enhanced dissolution rate of gold, in contrast to Cu(C 2 H 4 NO 2 ) 2 0 complex in near-nurture condition. Such excellent catalytic capability of the Cu(C 2 H 4 NO 2 ) 3 − complex for gold dissolution can be attributed to the lowest corrosion potential, slighter passivation behavior, and rapid electron transfer from the analysis of corrosion electrochemistry (OCP, PDP, and EIS) and advanced spectrum technologies (LA-ICP-MS and XPS). The dissolution of gold in the novel glycine-thiosulfate system undergoes a typical ligand exchange process (Au + →Au(C 2 H 4 NO 2 ) 2 − →Au(S 2 O 3 ) 2 3− ), and the formation of passivation species on the gold surface is predominantly formed by Au(I)-glycine coordination, rather than the sulfur-containing products from thiosulfate decomposition. Based on these results, the proposed two electrochemical reaction mechanisms will contribute to the optimization of leaching conditions in the novel glycine-thiosulfate system.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Xue Xia,Yun Zhai,Heping Cui,Han Zhang,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
A series of Amadori compounds of glucose were prepared from glycine (G-ARP), diglycine (DiG-ARP), and triglycine (TriG-ARP), and identified by UPLC-MS/MS and NMR. The formation rate of ARPs was TriG-ARP > DiG-ARP > G-ARP, and their activation energies were 63.48 kJ/mol (TriG-ARP), 72.84 kJ/mol (DiG-ARP), and 84.76 kJ/mol (G-ARP), respectively, suggesting that ARP was formed more easily from small peptides than from amino acid. Although 1-DG was formed much more difficultly than 3-DG, the same order of the formation of 1-DG, 3-DG, and browning was DiGly > TriGly > Gly. It was also confirmed that more methylglyoxal and glyoxal would be formed from small peptides than equimolar amino acids. Compared with free amino acid, ARP, deoxyglycosones, and their secondary degradation products were more easily formed from dipeptide and tripeptide, thereby stronger browning occurred and higher reactivity was exhibited in Maillard reaction of di- or tripeptide.
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CHEMICAL ENGINEERING JOURNAL
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2020
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IF 10.652
Ce Gao,LingPing Xiao,Jinghui Zhou,Haisong Wang,Shangru Zhai,Qingda An
A new type of nanosilver immobilized catalyst based on glycine modified lignin hydrogels with excellent catalytic performance towards p -nitrophenol reduction have been prepared. Glycine was used to provide amino and carboxy groups for complexing silver ions, simultaneously which was also beneficial for in-situ reduction and suppression of silver nanoparticles. As-prepared composites were characterized by X-ray diffraction, scanning electron microscope with EDS, high-resolution transmission electron microscope, X-ray photoelectron spectroscopy, Fourier Transform Infrared Spectrometer and Thermal Gravimetric Analyzer. The developed network with enriched anchoring sites could provide the stability during the catalytic process and enough traction to disperse and stabilize the silver nanoparticles. Batch experiments of 4-NP hydrogenation were carried out to correlate the catalytic performance. The concentration of 4-NP of 5 mmol/L could be finished with only 101 s and the rate of k was 0.0151 s −1 . Moreover, fixed-bed experiments were creatively applied for the tests and the catalyst can maintain high efficiency about 300 min with the flowing rate of 3 mL/min. After being reused ten cycles, the catalyst retained the conversion rate beyond 98%; and the structure has no obvious deterioration and the leaching of nanosilver was neglectful, which could be attributed to the crucial role of introduced glycine component.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2020
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IF 3.721
Haoran Wang,Ran Suo,Cuncun Zhang,Rongbin Li,Xu Shi,Yaqiong Liu,Wenxiu Wang,Jie Wang
A new method for determining the content of γ-glutamyl-valyl-glycine (γ-Glu-Val-Gly, γ-EVG) using high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) with precolumn derivatization by 2,4-Dinitrochlorobenzene (CDNB) was developed. The method exhibited satisfactory linear relationship ( R 2 = 0.9995) at concentrations ranging from 1.0 to 40.0 ng/mL. The limits of detection (LOD) and quantitation (LOQ) were 0.3 and 0.9 ng/mL, respectively. Recoveries of the target peptide in spiked samples were between 83.05% and 116.49%, showing the values of relative standard deviation (RSD) less than 10%. The intra-day and the inter-day precisions were calculated to be 7.13% and 8.54% ( n = 6), respectively. The validated approach was effectively applied to quantify the γ-EVG levels (0.13–0.38 μg/g) in different aquatic products. In conclusions, a sensitive, low-cost, convenient and reliable approach was developed for the detection of γ-EVG, which can serve as a potential tool for evaluating flavor peptide quality of aquatic products.
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ANALYTICAL LETTERS
·
2021
·
IF 2.329
Zhipeng Su,Fanyan Ye,Kaiyu He,Tao Yang,Wang Li,Jiali Ren
The sensitive determination of pesticides constitutes an important aspect in ensuring food safety. Due to the disadvantages of traditional methods, such as low sensitivity, complex operation, and high cost, the development of an efficient and economical biosensor to detect pesticide residues is required. In this work, the dipeptide glycyl-L-histidine (GH) and divalent copper ions (Cu2+) were employed as bioorganic linkers and metal ions in the synthesis of the peptide-based metal organic framework (MOF) material GH-Cu BioMOF. Because of its properties of fluorescence quenching and adsorption of nucleic acids, GH-Cu BioMOF was used to develop a distinctive aptamer based method for the determination of pesticide residues in food. The GH-Cu BioMOF-based aptasensor was used for acetamiprid and demonstrated a linear response in the range from 0.15 to 3.00 µM with a detection limit of 137 nM, verifying the feasibility of this method for pesticide determination in food.
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JOURNAL OF ORGANIC CHEMISTRY
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2019
·
IF 4.745
Jian Fan,Yinshan Ye,Guochao Chu,Zhongping Zhang,Yao Fu,Yi-Ming Li,Jing Shi
Ubiquitin (Ub)-based fluorescent reagents are crucial to explore the activity of deubiquitinases (DUBs). Ub-Rho110-G is one of the preferred tools, whereas the current synthetic route is time-consuming. Here, we report a new semisynthetic strategy to produce Ub-Rho110-G through direct aminolysis of Boc-protected Ub-Mesna using bisglycyl-rhodamine 110. We also applied this strategy to synthesize active SUMO2-Rho110-G for the first time. Biochemical analysis demonstrated that semisynthetic Ub or SUMO-Rho110-G can be effectively used for the detection of the activity of DUBs or SUMO-specific enzymes.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2015
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IF 2.416
Yueyu Zou,Caihuan Huang,Kehan Pei,Yun Cai,Guangwen Zhang,Changying Hu,Shiyi Ou
Inhibition of acrylamide formation in food has been extensively reported, but some mitigation methods result in a concomitant increase in hydroxymethylfurfural (HMF), a food contaminant mainly produced through Maillard reaction . Mitigation strategies to reduce HMF are not yet available. This study showed that cysteine alone or in combination with glycine could simultaneously and significantly reduce the content of acrylamide and HMF in asparagine/glucose model as well as in biscuits. Mixing 0.36 g/100 g of cysteine and 0.2 g/100 g (w/w) of glycine into the dough reduced 97.8% and 93.2% of acrylamide and HMF, respectively, in biscuits. Cysteine reduced HMF content possibly by reacting with formed HMF through Michael adduction and Maillard reaction.
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危险品化学品经营许可证（带存储）