Nature Communications
·
2024
·Vol. 15
·期 1
·1-15
·
IF 14.7
Li Hailong,Meng Fanyue,Zhu Penglin,Zu Hongxiao,Yang Zequn,Qu Wenqi,Yang Jianping
Highly efficient decontamination of elemental mercury (Hg 0 ) remains an enormous challenge for public health and ecosystem protection. The artificial conversion of Hg 0 into mercury chalcogenides could achieve Hg 0 detoxification and close the global mercury cycle. Herein, taking inspiration from the bio-detoxification of mercury, in which selenium preferentially converts mercury from sulfoproteins to HgSe, we propose a biomimetic approach to enhance the conversion of Hg 0 into mercury chalcogenides. In this proof-of-concept design, we use sulfur-rich polyphenylene sulfide (PPS) as the Hg 0 transporter. The relatively stable, sulfur-linked aromatic rings result in weak adsorption of Hg 0 on the PPS rather than the formation of metastable HgS. The weakly adsorbed mercury subsequently migrates to the adjacent selenium sites for permanent immobilization. The sulfur-selenium pair affords an unprecedented Hg 0 adsorption capacity and uptake rate of 1621.9 mg g −1 and 1005.6 μg g −1 min −1 , respectively, which are the highest recorded values among various benchmark materials. This work presents an intriguing concept for preparing Hg 0 adsorbents and could pave the way for the biomimetic remediation of diverse pollutants.
研究领域:
环境科学
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材料科学
·
化学工程
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2016
·
IF 3.782
Daqun Chen,Lei Zhao,Weihua Hu
Mussel inspired polydopamine (PDA) film has attracted great interest as a versatile functional coating for biomolecule immobilization in various bio-related devices. However, the details regarding the interaction between a protein and PDA film remain unclear. Particularly, there is very limited knowledge regarding the protein immobilization on PDA film, even though it is of essential importance in various fields. The situation is even more complicated if considering the fact that quite a number of approaches (e.g., different oxidizing reagent, buffer pH, grown time, grown media, etc.) have been developed to grow PDA films. In this work, protein attachment on PDA film was systematically investigated by using the real-time and label-free surface plasmon resonance (SPR) technique. The kinetics of protein-PDA interaction was explored and the influence of buffer pH and deposition media on the protein attachment was studied. Fluorescent protein microarray was further printed on PDA-coated glass slides for quantitative investigations and together with SPR data, the interesting fluorescence quenching phenomenon of PDA film was revealed. This work may deepen our understanding on the PDA-protein interaction and offer a valuable guide for efficient protein attachment on PDA film in various bio-related applications.
研究领域:
生物技术
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生物材料科学
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分析化学
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PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2026
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IF 7.8
Wang Xiufeng,Zhou Bo,Huang Yuhan,Wang Xiaoyu,Xu Shuping,Li Moyan,Wang Shirui,Yang Sizhuo,Cui Haiyang
Metal–organic frameworks (MOFs) are widely used for enzyme immobilization due to their tunable pore structures and high stability. However, whether a relatively universal MOF carrier can support diverse enzymatic reactions remains unclear. Herein, we systematically evaluated 120 MOF–enzyme–substrate combinations using ten representative MOFs, three hydrolases (CRL, CALB, and BsEst), and four ester substrates, and then extended the identified carriers to three additional enzymes spanning catalase, oxidase, and protease reactions (GOx, Typ, and BlCAT), examining each with a single substrate. Overall, UiO-66 and UiO-66-NH 2 showed the most consistently favorable relative apparent activity. Among the selected materials, UiO-66 and UiO-66-NH 2 emerged as universal carriers, enhancing catalytic activity by up to 1.6-fold compared with free enzymes under harsh conditions, including elevated temperatures, pH 3.5–10.5, and the presence of toxic molecules. Molecular dynamics simulations combined with the analysis of 45 structural, solvation, and energetic descriptors revealed two key mechanisms underlying the superior performance of Enzyme/UiO-66(–NH 2 ) systems: preservation of internal hydrogen-bond networks in both substrate-bound and substrate-free states, and framework-assisted substrate enrichment near active sites through enhanced adsorption. This work provides molecular insights into MOF–enzyme–substrate interactions and establishes potentially transferable design principles for the MOF-mediated immobilization of other enzymes.
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高影响因子
BIOTECHNOLOGY LETTERS
·
2013
·Vol. 35
·期 8
·1323-1330
·
IF 1.853
Guan Liye,Di Bin,Su Mengxiang,Qian Jie
The aminopropyl-functionalized ethane-bridged bifunctional periodic mesoporous organosilicas (APEPMOs) were synthesized by the co-condensation of 1,2-bis (triethoxysilyl) ethane and 3-aminopropyltriethoxysilane in the presence of cationic surfactants octadecyltrimethylammonium chloride in basic medium. The pores of the APEPMOs were expanded with N , N -dimethyldecylamine and the pore-expanded materials were utilized as supports for β - glucosidase immobilization. A high enzyme loading of 120 mg per g support was achieved in 18 h, and 95.5 % of enzymatic activity was retained. β-Glucosidases were strongly immobilized on APEPMOs with only 5 % desorption in the washing step with buffer solution. The immobilized enzyme had 75 % activity after 20 batch reactions and had improved thermal stability, relative to the free enzyme. These results demonstrate that APEPMOs would be promising supports for β-glucosidases immobilization.
研究领域:
生物化学
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材料科学
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CELLULOSE
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2025
·
IF 4.8
Yan Weimin,Hou Chengmin
Cationic copolymer carboxymethyl cellulose-graft-poly(methacryloxyethyl trimethylammonium chloride) (CMC- g -PDMC) was synthesized via free radical polymerization and further modified with glutaraldehyde (GA) to immobilize horseradish peroxidase (HRP). DMC, a cationic monomer, was grafted onto CMC to enhance electrostatic adsorption, while GA provided covalent crosslinking sites for HRP. The GA-crosslinked copolymer (CMC- g -PDMC-GA) enabled efficient enzyme immobilization on glass surfaces for batch and continuous catalysis. After 10 cycles of batch catalysis, the immobilized HRP retained 98% of its initial activity. In continuous flow (2 h), the activity remained stable, with a 1.6-fold higher efficiency on NaOH-treated glass compared to untreated surfaces. HRP alone or without GA crosslinking showed negligible immobilization.
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Materials Today Communications
·
2026
·
IF 4.9
Yadong Wang,Linyan Li,Zhenming Lu,Shengdong Zhang,Yi Liu,Jingyi Ding
The waste molten salt (LiCl-KCl) generated from high-temperature pyrometallurgical processing of spent fuel has high water solubility and strong corrosiveness. Thus, it is difficult to be effectively solidified with traditional matrices, such as cement and glass. In this study, high-temperature solid-phase reactions were carried out between reactive aluminosilicate precursors and LiCl, KCl, LiCl-KCl, and their oxalate-transformed products (Li 2 C 2 O 4 -K 2 C 2 O 4 , NaCl) to synthesize ceramic waste forms containing Li + , K + , and Cl - . The phase composition, morphology, and elemental distribution of the solidified products were characterized using XRD, SEM/EDS, ICP, and ion chromatography. The results indicate that: (1) When directly solidifying LiCl-KCl, the solidification rates of Li + and K + were relatively high, while that of Cl - was comparatively low; (2) Using oxalate-transformed products (Li 2 C 2 O 4 -K 2 C 2 O 4 , NaCl) obtained by heating the simulated molten salt of LiCl-KCl mixed with oxalic acid as starting materials, the solidification rates of Li + , K + , and Cl - are significantly increased; (3) the leaching rates of Li + , K + , Na + , and Cl - of the aforementioned solidification bodies are all below 7.0 × 10 −4 g·m −2 ·d −1 .
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ANALYTICA CHIMICA ACTA
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2011
·
IF 4.311
Chunhe Yao,Li Qi,Wenbin Hu,Fuyi Wang,Gengliang Yang
A new kind of immobilized trypsin reactor based on sub-micron skeletal polymer monolith has been developed. Covalent immobilization of trypsin on this support was performed using the epoxide functional groups in either a one- or a multi-step reaction. The proteolytic activity of the immobilized trypsin was measured by monitoring the formation of N -α-benzoyl- l -arginine (BA) which is the digestion product of a substrate N -α-benzoyl- l -arginine ethyl ester (BAEE). Results showed that the digestion speed was about 300 times faster than that performed in free solution. The performance of such an enzyme reactor was further demonstrated by digesting protein myoglobin . It has been found that the protein digestion could be achieved in 88 s at 30 °C, which is comparable to 24 h digestion in solution at 37 °C. Furthermore, the immobilized trypsin exhibits increased stability even after continuous use compared to that in free solution. The present monolithic enzyme-reactor provides a promising platform for the proteomic research.
研究领域:
生物化学
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蛋白质组学
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酶工程
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分析化学
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高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 136
·期 29
·47784
·
IF 2.188
Jing Sun,Caihong Wang,Yanzi Wang,Shuxin Ji,Wenfang Liu
Carbonic anhydrase (CA) catalyzing CO 2 hydration has an important application in carbon capture, and its immobilization is very significant. Here, CA was covalently linked by glutaraldehyde (GA) to the surface of poly(vinylidene fluoride) (PVDF) and polyethylene (PE) membranes, which were previously modified via a simple codeposition of polyethyleneimine (PEI) and dopamine (DA). The effects of the modification conditions were investigated, and the membranes were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. The immobilization process was optimized, and the catalytic properties of immobilized CA were studied. The results show that the optimal mass ratio of PEI and DA was 1:1 and the deposition time was 10–12 h, at which the surface amino group density could reach 1.278 × 10 −7 and 1.397 × 10 −7 mol/cm 2 for PVDF and PE, respectively. For enzyme immobilization, the optimal CA and GA concentrations were 0.2 mg/mL and 0.1 wt %, and a maximum activity recovery of about 53% and 76% could be achieved for PVDF-attached CA and PE-attached CA, respectively. Their K m values were 10.62 m M and 8.6 m M , and the corresponding K cat / K m values were 132.2 M −1 s −1 and 312.9 M −1 s −1 . After immobilization, the storage stability and reusability of CA were much improved. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47784.
研究领域:
生物化学
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材料科学
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环境科学
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BIOCHEMICAL ENGINEERING JOURNAL
·
2013
·
IF 2.579
Qinghong Ai,Dong Yang,Yuanbing Li,Jiafu Shi,Xiaoli Wang,Zhongyi Jiang
In this study, titanate nanotubes (TNTs) with desirable biocompatibility and hydrophilicity have been synthesized by a facile and cost-effective alkaline hydrothermal method, and used to immobilize the enzyme. The characterization results reveal that the prepared TNTs have a regular tubular morphology with a length about 100–180 nm and an outer diameter about 10 nm, and a BET specific surface area of 305.4 m 2 g −1 . Catalase (CAT), as the model enzyme, was pre-modified by 3-(3,4-dihydroxyphenyl) propionic acid (3,4-diHPP) via 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) coupling chemistry, and then covalently immobilized on the TNTs surface by the chelation of catechol groups with Ti 4+ ions. It is found that TNTs exhibits excellent performances as the immobilized supporter of enzyme: the enzyme loading is as high as 820 mg g of support −1 ; the relative activity of immobilized enzyme is about 60% of that of free enzyme; the immobilized CAT demonstrates enhanced storage and recycling stability.
研究领域:
生物化学
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纳米材料科学
·
酶工程
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DOI
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2019
·
IF 4.046
Xiaohai Liu,Baoguo Ma,Hongbo Tan,Hainan Li,Junpeng Mei,Ting Zhang,Pian Chen,Benqing Gu
The utilization of marine resources in reinforced concrete is severely limited, due to the risk of steel corrosion caused by the introduced chloride. In order to reduce this risk, nano-Al 2 O 3 (NA) was added into cement-based materials system by improving the chloride immobilization. The immobilized chloride ratio (ICR) of the system was evaluated, and the results indicated that the addition of NA could significantly enhance ICR. Based on the results from TG, XRD, 27 Al NMR, MIP, 29 Si NMR, and EDS, the mechanism behind was discussed from three aspects of chemical binding, migration resistance, and physical adsorption. The reasons for the increased ICR were as follows: a) chemical binding was promoted by increasing the amount of chloroaluminates; b) migration resistance was improved by refining the pore structure; c) physical adsorption, depending on the amount and the Ca/Si ratio of C-S-H gel, was reduced negligibly. These findings can provide guidance for the theoretical study of chloride immobilization and the application of marine resources.
研究领域:
土木工程
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材料科学
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Wen-Ze Qiu,Zi-Shu Zhao,Yong Du,Meng-Xin Hu,Zhi-Kang Xu
Biofouling control is a major task in membrane separation processes for water treatment and biomedical applications. In this work, N -alkylated polyethylenimine (PEI) is facilely and efficiently introduced onto the membrane surfaces via the co-deposition of catechol (CCh) and PEI, followed by further grafting of PEIs (600 Da, 70 kDa and 750 kDa) and cationization with methyl iodide (CH 3 I). The physical and chemical properties of the constructed membrane surfaces are characterized with scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, zeta potential and water contact angle measurements. Antibacterial assay reveals that the optimized membrane surfaces possess around 95% antibacterial efficiency against Gram-positive Staphylococcus aureus ( S. aureus ) with weak adhesion of bacteria cells after 24 h of bacterial contact. Additionally, the membrane surfaces also exhibit much enhanced antifouling property during the filtration of opposite charged bovine serum albumin (BSA). These results demonstrate a useful strategy for the surface modification of separation membranes by a kind of antimicrobial and antifouling coating.
研究领域:
环境科学
·
生物医学工程
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RSC Advances
·
2018
·Vol. 8
·期 38
·21084-21091
·
IF 2.936
D. Zhang,X. Y. Liu,H. T. Zhao,L. Yang,T. Lü,M. Q. Jin
Radioactive iodine is quite mobile in soil and poses threats to human health and the ecosystem. Many materials, including layered double hydroxides (LDH), have been synthesized to successfully capture iodine from aqueous environments. However, limited information is available on the application of LDH in soil to immobilize iodine species. In the present study, the feasibility of using Mg–Al–NO3 LDH for retention of soil iodate (IO3−) in both batch and column systems was analyzed. The 2 : 1 Mg–Al–NO3 LDH exhibited the greatest removal efficiency of IO3− from aqueous solution, compared with 3 : 1 and 4 : 1 Mg–Al–NO3 LDH. The Mg2–Al–NO3 LDH demonstrated a strong affinity for IO3−, with a high sorption capacity of 149 528 mg kg−1 and a Freundlich affinity constant KF of 21 380 L kg−1. The addition of Mg2–Al–NO3 LDH in soil resulted in significant retention of IO3− in both the batch and column experiments. The affinity parameter KF of soil with the addition of 1.33% Mg2–Al–NO3 LDH was 136 L kg−1, which was 28.6 times higher than soil without LDH added. Moreover, the eluted iodate percentage was only 12.9% in the soil column with the 1.33% Mg2–Al–NO3 LDH addition, whereas almost 43.5% iodate was washed out in the soil column without LDH addition. The results suggested that Mg2–Al–NO3 LDH could effectively immobilize iodate in soil without obvious interference.
研究领域:
环境科学
·
放射性污染控制
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PubMed
高影响因子
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