Antioxidants
·
2026
·Vol. 15
·期 9
·1207
·
IF 8.2
Kaiwen You,Yixuan Li,Wanqing Zhong,Jie Yu,Yamei Wu,Yuqi Guo,Haoyang Liu,Haiping Xie,Kai Yuan,Yihong Chen,Lei Wang
Due to the hypoxic environment caused by global warming and intensive aquaculture process, fish are susceptible to oxidative damage and death.Eucommia ulmoidesextract (EUE) has demonstrated antioxidant and anti-inflammatory properties. However, whether EUE can mitigate hypoxia-induced negative effects remains uninvestigated in fish. This study investigated the effect of 0 and 1.0 g/kg EUE on acute hypoxia-induced damage in largemouth bass and its potential mechanisms. The results showed that EUE alleviated the deterioration of plasma activities (ALT and AST) induced by acute hypoxic stress, decreased the number of macrophages and increased the total number of blood cells. EUE was effective in reducing the level of apoptosis and the content of ROS, Ca2+, and NO in blood cells. Additionally, EUE alleviated liver tissue injury caused by acute hypoxic stress, which could be associated with increased total antioxidant capacity and antioxidant enzyme activities (SOD, CAT and GSH-Px). Furthermore, EUE up-regulated the expression levels ofNrf2andCAT, activatedXBP1andIREexpression and down-regulatedGRP78expression, relieving the endoplasmic reticulum (ER) stress response. The down-regulation ofCaspase3andCaspase9is evidence that EUE inhibited apoptosis in acute hypoxic stress. In summary, EUE reduced oxidative damage, ER stress, and apoptosis caused by hypoxic stress via the Nrf2/Keap1 signaling pathway in largemouth bass. This study presents a part of the theoretical foundation for the EUE to resist damage caused by hypoxic stress, thereby establishing a basis for sustainable development in aquaculture.
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高影响因子
AQUACULTURE
·
2021
·
IF 4.242
Peng Yin,Shiwei Xie,Zhenxiao Zhuang,Haohan Fang,Lixia Tian,Yongjian Liu,Jin Niu
High-fat diets (HFD) is widely applied in aquaculture to achieve a protein-saving effect, whereas long-term intake of high-fat feed will negatively affect the health of fish. Chlorogenic acid (CGA) is an effective antioxidant and anti-obesity substance. However, whether CGA can address a series of adverse effects caused by fish's high lipid diet is unclear. Four diets were formulated: a control diet (C) with approximate 11% lipid; HFD with 18% lipid (HF) and HFD supplemented with 300 and 600 mg/kg CGA (HFC1 and HFC2, respectively) and a nine-week feeding trial was conducted of largemouth bass . Here we show that dietary CGA improves health of largemouth bass by regulating expression of gene responsible for lipid metabolism , inflammation, and antioxidant enzymes. We found that dietary CGA decreases total triglyceride (TG) and total cholesterol (TC) in plasma and nonestesterified fatty acid (NEFA) in liver of largemouth bass, and 600 mg/kg CGA increased mRNA expression levels of lipolysis such as hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL), and apolipoprotein A1 (APOA1) in the liver. In addition, we found CGA downregulates mRNA expression levels of interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α). Furthermore, we found that CGA decreases malondialdehyde (MDA) level of liver of largemouth bass, while increased mRNA expression of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT). Our results indicate that CGA plays a role in lipid metabolism, inflammation and antioxidant capacity of largemouth bass fed HFD.
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高影响因子
AQUACULTURE
·
2019
·
IF 3.022
Yves Harimana,Xue Tang,Pao Xu,Gangchun Xu,Eric Karangwa,Kai Zhang,Yongjuan Sun,Yingrui Li,Shuhua Ma,Angelo Uriho,Marie Alice Tuyishimire
Fish fillet is heavily influenced by swimming exercise and other factors leading to a great change in fish physiology. During the present study, muscle cellularity, texture and composition, antioxidant activities and flavor characteristics were assessed and compared between non-exercised (N-EXF) and long-term exercised Largemouth bass . Exercised fish (EXF) showed improve in muscle cellularity and texture with high protein, hydroxyproline , collagen and low crude lipid compared to N-EXF. In addition, EXF showed Higher INOS, T-AOC, CAT and lower MDA, indicating high antioxidant activities in EXF compared to N-EXF. Furthermore, EXF showed lower hydrocarbons and alcohols content but higher in carboxylic acid, esters and aromatic compounds content compared N-EXF. EXF also showed lower TMAO , TMA, HX and high IMP content, indicating better freshness. Generally, EXF largemouth bass should be preferred depends on its high nutrition value, better flesh and freshness quality; and pleasant flavor.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Junde Ren,Feifei Hu,Farooq Muhammad,Lihui Hu,Tao Song,Maninder Meenu,Jie Lin,Ying Liu,Hosahalli S. Ramaswamy,Yong Yu
This study investigated the freezing characteristics of Arctic yeast Leucosporidium sp. AY30 ice-binding protein (LeIBP) after heterologous expression by Aspergillus niger at different concentrations (0.01 %, 0.05 %, 0.1 %, 0.2 %, 0.5 %) and elucidated its effects on physicochemical quality, myofibrillar protein (MP) and ice crystal morphology of largemouth bass during air freezing. LeIBP achieved ice crystal regulation by significantly reducing the freezing point, super-cooling degree and phase transition time of the aqueous solution and prolonging the time of the maximum ice crystal formation zone (−1 to −5 °C). Its thermal hysteresis activity (THA) reached a peak at 0.2 % concentration (1.36 °C), which was superior to the THA of most traditional AFPs. Notably, the addition of 0.05 %LeIBP dramatically reduced the cross-sectional area and mean diameter of ice crystals by up to 91 % and 70 %, respectively. In addition, the addition of LeIBP better maintained the texture of fish, led to an increase in the L* value and a decrease in the b* value, and inhibited the decrease in the a* value, but decreased its water holding capacity slightly. Meanwhile, the addition of 0.01 %LeIBP better inhibited the reduction of MP content and maintained the stability of MP tertiary structure. The addition of LeIBP increased the α-helix content by 1.0–6.9 % to maintain the MP secondary structure, also significantly increased the ion bond content, hydrogen bond content, and hydrophobic interactions. In summary, the addition of low concentration (0.01 % and 0.05 %) of LeIBP better maintained the quality of fish meat during air freezing.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2025
·
IF 5.2
Xianhao Zhang,Bingrui Guo,Bing Dai,Shimiao Wang,Siqi Zhang,Sufang Zhang,Chaofan Ji,Beiwei Zhu,Xinping Lin
In this study, the effect of fermentation on the safety, flavor, and texture of largemouth bass (Micropterus salmoides ) was investigated. Compared to fresh fish, the proteins formed a network structure that enhanced bound water proportion, texture hardness, and water holding capacity, while it reduced cooking losses. TVB-N and TBARS levels increased gradually but stayed within safe limits. However, histamine accumulation surpassed the recommended limit at 56.84 ± 0.17 mg/100 g in late stages. A total of 67 volatile compounds were detected, with 13 identified as key flavor compounds (OAV > 1). 3rd-generation full-length 16S rRNA gene sequencing revealed a microbial community shift from high diversity to dominance by strains like Vibrio anguillarum , Vagococcus fessus , and Vagococcus coleopterorum . Among these, 10 species were highly correlated with flavor formation. This study reveals quality patterns in fermented largemouth bass, providing insights into achieving a balance among safety, flavor, and texture in fermented fish.
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PubMed
高影响因子
AQUACULTURE
·
2026
·
IF 4.4
Yu-Yang Liang,Ying-Man Yu,Chen-Chen Wu,Samwel M. Limbu,Meng-Ting Liu,Fang Qiao,Mei-Ling Zhang,Zhen-Yu Du,Yuan Luo
The expansion of intensive aquaculture and shifts in feed ingredient supply and demand have driven the adoption of high-energy feeds, resulting in metabolic disorders and diminished flesh quality in farmed fish. The natural compound trimethylamine oxide (TMAO) shows great promise as a functional feed additive, such as dietary TMAO supplementation significantly reduces the abdominal fat ratio in bluntnose black bream ( Megalobrama terminalis ), and decreases crude lipid content in the liver of Nile tilapia ( Oreochromis nilotica ), yet its effects and mechanisms in carnivorous fish such as the largemouth bass ( Micropterus nigricans ) remain unclear. This study investigated the effects of TMAO at different supplementation levels on the growth performance, lipid metabolism, fish flesh quality, and targeted metabolomics of largemouth bass. A 56-day feeding trial was designed with three TMAO supplementation levels (0.1%, 0.2%, and 0.4%). A total of 360 largemouth bass (8.78 ± 2.9 g) were randomly assigned to four treatment groups, each with three replicates. TMAO supplementation at all levels had no effect on growth performance. TMAO reduced hepatic lipid deposition in largemouth bass with a concentration-dependent effect, upregulating fatty acid β-oxidation gene expression. Targeted metabolomics analysis further revealed that TMAO increased carnitine content and elevated methylation levels by increasing SAM content in the liver. Furthermore, TMAO increased fish flesh hardness, promoted muscle fiber growth and development, enhanced muscle fiber density, and upregulated gene expression in the mTOR pathway. This study elucidates that dietary TMAO supplementation promotes lipid metabolism by increasing carnitine levels in the liver, and enhances muscle hardness by promoting muscle fiber growth and development.
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高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2026
·
IF 4.2
Yujun Liu,Gengrong Zhang,Haozhe Qu,Ziyu Hu,Maosheng He,Fei Ling,Gaoxue Wang,Tianqiang Liu
The mRNA vaccine can induce specific antibodies in Largemouth bass. • mRNA Cap (1) and poly (A) mRNA reduce RIG-I recognition of foreign mRNA in Largemouth bass. • The mRNA vaccine triggered a rapid immune response in bony fish. • Improved the survival rate of largemouth bass to 57% in LMBV infection. Largemouth bass virus (LMBV) infection poses a significant threat to largemouth bass farming in China, yet effective and safe vaccines remain scarce. In this study, we designed and evaluated four mRNA vaccine candidates against LMBV, all encoding the viral major capsid protein (MCP). The fully configured construct (LMUM) contained a 5′ cap1, untranslated regions (UTRs), and a poly(A) tail, and was produced by in vitro transcription. Each mRNA was encapsulated into lipid nanoparticles (LNPs), yielding homogeneous particles (∼110 nm diameter, ∼54 mV zeta potential) with >96% encapsulation efficiency that provided substantial protection against RNase degradation (43% of mRNA remained intact in LNP-encapsulated groups versus 10% in unprotected controls). Intramuscular immunization of largemouth bass with LNP-encapsulated LMUM (0.2 μg/g body weight) induced MCP-specific serum antibodies after primary immunization, with a strong booster response after the second dose. In the LMUM-immunized group, spleen mRNA levels of IgM and CD4 were upregulated 3.4-fold and 3.2-fold, respectively, by day 14, while the groups lacking UTR or cap/poly(A) tail failed to induce these adaptive immune markers. Interestingly, the incomplete constructs triggered stronger activation of the RIG-I pathway (RIG-I, MAVS, TBK1) than the fully modified LMUM, suggesting that the combination of cap1, UTR, and poly(A) tail helps dampen excessive innate sensing. Following a lethal LMBV challenge, the LMUM vaccine conferred 45–50% relative survival after primary immunization, which increased to 53–57% after the booster dose. In contrast, the incomplete vaccine constructs and naked mRNA gave only 10–15% survival, and control groups experienced nearly complete mortality. These results demonstrate that a fully modified mRNA-LNP vaccine against LMBV induces robust humoral and cellular immune responses and provides significant protective efficacy in largemouth bass.
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PubMed
高影响因子
Frontiers in Immunology
·
2023
·
IF 7.3
Li Lukuan,Wang Yu,Huang Yanqing,Wang Chunfang
Introduction: In an effort to minimize the usage of fishmeal in aquaculture, novel protein diets, including Tenebrio molitor, cottonseed protein concentrate, Clostridium autoethanogenum, and Chlorella vulgaris were evaluated for their potential to replace fishmeal. Nevertheless, comprehensive examinations on the gut health of aquatic animals under an alternate feeding strategy when fed novel protein diets are vacant.Methods: Five isonitrogenous and isolipidic diets containing various proteins were manufactured, with a diet consisting of whole fishmeal serving as the control and diets containing novel proteins serving as the experimental diets. Largemouth bass (Micropterus salmoides) with an initial body weight of 4.73 ± 0.04g employed as an experimental animal and given these five diets for the first 29 days followed by a fishmeal diet for the next 29 days.Results: The results of this study demonstrated that the growth performance of novel protein diets in the second stage was better than in the first stage, even though only the C. vulgaris diet increased antioxidant capacity and the cottonseed protein concentrate diet decreased it. Concerning the intestinal barriers, the C. autoethanogenum diet lowered intestinal permeability and plasma IL-1β/TNF-α. In addition, the contents of intestinal immunological factors, namely LYS and sIgA-like, were greater in C. vulgaris than in fishmeal. From the data analysis of microbiome and metabolome, the levels of short chain fatty acids (SCFAs), anaerobic bacteria, Lactococcus, and Firmicutes were significantly higher in the C. autoethanogenum diet than in the whole fishmeal diet, while the abundance of Pseudomonas, aerobic bacteria, Streptococcus, and Proteobacteria was lowest. However, no extremely large differences in microbiota or short chain fatty acids were observed between the other novel protein diets and the whole fishmeal diet. In addition, the microbiota were strongly connected with intestinal SCFAs, lipase activity, and tight junctions, as shown by the Mantel test and Pearson’s correlation.Discussion: Taken together, according to Z-score, the ranking of advantageous functions among these protein diets was C. autoethanogenum diet > C. vulgaris diet > whole fishmeal diet > cottonseed protein concentrate > T. molitor diet. This study provides comprehensive data illustrating a mixed blessing effect of novel protein diets on the gut health of juvenile largemouth bass under an alternate feeding strategy.
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PubMed
高影响因子
AQUACULTURE
·
2022
·
IF 5.135
Lukuan Li,Yu Wang,Zhen Zhang,Chunfang Wang
The novel proteins, i.e., Clostridium autoethanogenum (CAP), Tenebrio molitor (TM), Chlorella vulgaris (ChM), and cottonseed protein concentrate (CSM), are promising food for the future aquaculture due to the limited fishmeal (FM) resources. However, in-depth investigations on the intestinal barriers of fish when fed novel proteins are sparse, and the association between intestinal flora and physiological biomarkers is still unknown. Five isonitrogenous and isolipidic diets comprising various proteins were developed, with fishmeal serving as the control and novel proteins as the experimental diets. Largemouth bass ( Micropterus salmoides ) with an initial body weight of 211.9 ± 1.2 g were used as experimental model and fed with these five diets for eight weeks. The findings from the current study indicated that the intestinal histological structure as well as immunochemistry barrier performance of the ChM, CAP, and TM groups were superior to those of the CSM and FM groups, despite the fact that the villi height of FM was the greatest of all groups. Similarly, the intestinal inflammatory response and the permeability were elevated in CSM while decreased in ChM, CAP, and TM group. Concerning the intestinal short fatty acids (SCFAs), the levels in the CAP as well as ChM group were greater than the other proteins. From the data analysis of 16S rRNA gene sequencing, the abundance of opportunistic pathogens including (Ralstonia , Acinetobacter , Aeromonas , and Protebacteria) were higher in CSM group while attenuated in ChM, CAP and TM group. In addition, the abundance of nine types of intestinal-barriers-repairing probiotics were elevated in all novel proteins compared with the fishmeal group. These altered microbiota were highly correlated with intestinal inflammatory response, antioxidant status, immunochemistry barrier, physical barrier and SCFAs indexes by Mantel test and Pearson's correlation analysis. Collectively, the ranking of the beneficial functions among these five proteins was ChM > CAP>TM > FM > CSM according to Z -score and this study provides in-depth data manifesting the effects of novel proteins on the gut health status of large-size largemouth bass.
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高影响因子
Aquaculture Reports
·
2023
·
IF 3.7
Kai Luo,Yuhui Shi,Kunlin Yang,Qiaoqing Xu,Jiang Zhou,Weihua Gao,Chenglong Wu,Zhuoduo Wang
This study aimed to investigate the impact of dietary tributyrin (TB) in the low fish meal (high- Clostridium autoethanogenum protein, CAP) diet on the growth, antioxidation, lipid metabolism and intestinal microbiota of largemouth bass ( Micropterus salmoides ). For eight weeks, largemouth bass (15.05 ± 0.58 g) were fed on eight isonitrogenous and isolipid diets with graded levels of dietary TB (0 g/kg, 0.2 g/kg, 0.4 g/kg, 0.8 g/kg, 1.6 g/kg, 3.2 g/kg, 4.0 g/kg and 8.0 g/kg). The findings showed that the weight gain rate (WGR) and specific growth rate (SGR) were increased by 1.6 g/kg of dietary TB. The protein efficiency and deposition ratios were linearly affected by dietary TB. The broken-line model based on the WGR revealed that 1.617 g/kg of dietary TB was sufficient for optimal growth. The digestive enzyme activities and villus length of the intestine were increased by dietary TB (1.6 g/kg). Total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and catalase (CAT) activities were significantly improved, and the malondialdehyde (MDA) and protein carbonyl (PC) contents were significantly decreased after dietary TB. The expressions of nuclear factor erythroid 2-related factor 2 ( nrf2 ) and kelch like ech associated protein 1 ( keap1 ) in the liver and intestine were significantly increased and decreased by 1.6 g/kg of dietary TB, respectively. The mRNA levels of copper-zinc superoxide dismutase ( cuznsod ), cat , heme oxygenase-1 ( ho-1 ) and glutathione peroxidase ( gpx ) were significantly upregulated. Dietary TB could significantly affect the total protein (TP) and albumin (ALB) content in the serum. The aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were the lowest at 1.6 g/kg of TB group. Besides, the hepatocyte vacuolation and nuclear translocation were alleviated after supplementation of 0.2‰− 3.0‰ of dietary TB. The total cholesterol (T-CHO), high density lipoprotein cholesterol (HDLC) and low density lipoprotein cholesterol (LDLC) were significantly increased by dietary TB. The triglycerides (TG) content was decreased by dietary TB. The mRNA levels of sterol regulatory element-binding protein 1-c ( srebp1-c ), acetyl-coa carboxylase alpha ( accα ), fatty acid synthase ( fasn ) and accβ in the liver were significantly downregulated, and peroxisome proliferator-activated receptor alpha ( pparα ) and carnitine palmitoyltransferase 1 ( cpt-1 ) were upregulated by 1.6 g/kg of dietary TB. Dietary TB had effects on the intestine microbes of largemouth bass. The Cetobacterium was present in the highest percentage and increased from 41% (0 g/kg TB) to 59% (1.6 g/kg TB). The Mycoplasmataceae was the second most predominant genus and decreased from 90% in the 0 g/kg of TB group to 10% in the 1.6 g/kg of TB group. In summary, the dietary TB could be supplemented in the CAP-based diet for better growth and antioxidation, lipid metabolism and intestinal microbiota of largemouth bass.
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高影响因子
AQUACULTURE RESEARCH
·
2021
·Vol. 52
·期 7
·2963-2979
·
IF 2.082
Qin Ni,Chunfang Cai,Shengjie Ren,Junbiao Zhang,Yijun Zhao,Xinyi Wei,Yiyang Ge,Chengrui Wang,Wenjian Li,Ping Wu,Yuantu Ye,Xiamin Cao
This study aimed to reveal the impact of excessive dietary fibre (DF) intake on inflammatory responses and bile acid (BA) homeostasis in largemouth bass ( Micropterus salmoides ) and the potential mechanism. In vitro BA binding capacity was compared, and the results showed that the BA binding capacity to chenodeoxycholic acid and glycocholic acid by pectin and soybean meal was higher than cellulose and cottonseed meal respectively. An in vivo trial was conducted using a diet without DF as a control (CON diet), while test diets contained 150 g/kg cellulose (CEL diet) or pectin (PEC diet), 500 g/kg soybean meal (SBM diet) or cottonseed meal (CSM diet). Fish were overfed and ate ad libitum . Compared to fish fed the CON diet, fish fed the PEC and SBM diets showed high expression of inflammatory factor genes and BA synthesis factor genes and obvious histopathological damage in the gut and liver, while the responses of fish fed the CEL and CSM diets were mild. Whole-body BA contents in fish fed all test diets were lower than those in fish fed the CON diet ( p < 0.05). These results support the hypothesis that excessive DF intake induces inflammatory responses and dysregulation of BA homeostasis, which might be attributed to their binding capacity to BAs.
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高影响因子
危险品化学品经营许可证（带存储）