MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Jun Zhao,Xinhui Gu,Jiyang Liu
A highly sensitive electrochemiluminescence (ECL) aptasensor was developed for the detection of carcinoembryonic antigen (CEA), a well-established broad-spectrum tumor biomarker, through the integration of nanochannel-confined nanocatalysts, endogenous coreactants, and nanochannels with anti-fouling ability. A silica nanochannel film (SNF) with uniform pore size (2–3 nm) was grown on an indium tin oxide (ITO) electrode via simple Stöber solution growth method. Nitrogen-doped graphene quantum dots (N-GQDs) were subsequently confined within the nanochannels through electrophoretic deposition. The spatial confinement significantly enhanced the catalytic activity of N-GQDs toward luminol oxidation and dissolved oxygen (DO) reduction, thereby significantly amplifying the ECL signal without the need for additional coreactants. The outer surface of SNF was functionalized with epoxy groups using a silane coupling agent, allowing for covalent immobilization of the CEA-specific aptamer to construct a selective recognition interface. Upon target binding, steric hindrance and increased interfacial resistance hindered luminol diffusion and electron transfer, resulting in ECL signal suppression. Under optimized conditions, the constructed aptasensor exhibited a linear response toward CEA in the range of 0.001–10 ng/mL with a detection limit of 0.4 pg/mL (S/N = 3). The combined use of endogenous O₂ as a coreactant and the anti-fouling properties of the SNF enabled direct and reliable detection of CEA in complex serum samples without the need for complicated pretreatment. This work presents a versatile sensing platform based on nanoconfinement, catalyst enhancement, and antifouling interfaces for sensitive clinical biomarker detection.
研究领域:
分析化学
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生物化学
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纳米技术
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生物传感器
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肿瘤学
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材料科学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2025
·
IF 2.5
Xueru Zhang,Jiarong Guo,Wei Tang,Xinrui Duan
Based on the chemical structure and luminescence mechanism of luminol, the synthesis of luminol analogues has been widely studied. However, luminol and its derived reagents are still suffering from some drawbacks, such as short luminescence time and weak intensity. Herein, we designed and synthesized a novel hydroxyphenyl benzothiazole-based phthalhydrazide derivative DAH-AMPH, which has a hydroxyphenyl benzothiazole structure as a strong fluorescent site and a cyclic phthalhydrazide structure as a chemiluminescence (CL) emitting site. DAH-AMPH exhibited excellent CL properties in the DAH-AMPH/UHP/hemin CL system for hemin detection with a wide linear range of 0.1 nM to 20.0 nM and low LOD as 3 pM. It performed good selectivity in bloodstain imaging on different substrate materials. DAH-AMPH/UHP/hemin CL system supplied a powerful tool for trace detection of bloodstain. Moreover, as a new CL substrate to HRP, DAH-AMPH showed high sensitivity (0.8 pg/mL of LOD) in the DAH-AMPH/UHP/SPTZ/MORP/HRP CL system indicating a bright prospect for the use of DAH-AMPH in heme-containing peroxidase detection.
研究领域:
分析化学
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法医学
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生物化学
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产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 10
·1135-1140
·
IF 1.618
Wang Ze,Dong Bin,Feng Guodong,Shan Hongyan,Huan Yanfu,Fei Qiang
In the present study, we synthesized a water-soluble substance (Hemin-mPEG) at room temperature by using hemin and poly(ethylene glycol) methyl ether (mPEG). It was found that the Hemin-mPEG maintained the excellent catalytic activity inherited from hemin, and was first used to catalyze a luminol–H 2 O 2 chemiluminescence (CL) system to generate an intense and slow CL signal. The results of a mechanism research showed that the presence of Hemin-mPEG could promote the production of oxygen-relative radicals from H 2 O 2 and dissolved oxygen in solution. Based on this mechanism, an ultra-sensitive, cheap and simply practical sensor for detecting glucose and H 2 O 2 was developed. Under the most optimal experimental conditions, H 2 O 2 and glucose detection results exhibited a good linear range from 0.002 to 3 μM and from 0.02 to 4 μM, respectively, and the detection limits were 1.8 and 10 nM, respectively. This approach has been successfully used to detect glucose in actual biological samples, and achieved good results.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Dejian Li,Sisi Zhang,Xuan Feng,Hongjing Yang,Fei Nie,Wenyan Zhang
In this work, a metal organic framework material ([Co(L)(H 2 O) 2 ] n , Co-MOF) was synthesized by a facile hydrothermal method, and it was found that Co-MOF have a peroxidase-like activity. They can efficiently catalyze the chemiluminescence (CL) reaction between luminol and H 2 O 2 and enhance the CL signal significantly. The possible enhancement mechanism suggested that Co-MOF catalyzed the decomposition of H 2 O 2 into O 2 and increased the generation of •O 2 − . Based on these findings, a new highly sensitive glucose sensor was constructed combined with the luminol-H 2 O 2 -Co-MOF CL system and glucose oxidase. Under the optimal conditions, the method exhibited a good linear response to glucose in the range of 0.04˜8 μM with a detection limit of 1 × 10 -8 M. The method has been further applied in the quantitative determination of glucose in human serum and urine successfully.
研究领域:
分析化学
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生物传感
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材料科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Hui Ouyang,Mengyao Wang,Wenwen Wang,Zhifeng Fu
Luminol-reduced gold nanoparticles (LuReGNPs) were prepared and utilized as dual-response signal probes for the development of a colorimetric/chemiluminescent immunochromatographic test strip (ITS). LuReGNPs-labeled antibody was adopted as the signal tracer to conduct immunoassay of mouse IgG using the developed ITS device. After 6-min competitive immunoreaction, the tracer antibody was captured on the test zone, which led to accumulation of LuReGNPs. The appearance of red color resulting from LuReGNPs accumulation on the test zone was observed by naked eyes as a qualitative and semi-quantitative signal. Moreover, luminophore in the signal probes enabled sensitive quantitation after initiating the luminol-H 2 O 2 chemiluminescent reaction. Under the optimal conditions, mouse IgG was detected within a linear range of 0.10–100 ng/mL with a detection limit of 0.033 ng/mL (S/N = 3). The results for real biological samples detection and recovery tests demonstrated the acceptable reliability of the dual-response ITS. It exhibited great potential in both rapid screening and sensitive quantitation. Due to the merits of facile manipulation, short detection time and ideal portability, the platform could be further extended to multianalyte immunoassay of biomarker proteins for point-of-care test.
研究领域:
分析化学
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纳米技术
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生物医学工程
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即时检测
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2018
·
IF 2.275
Malik Saddam Khan,Wenjuan Zhu,Asghar Ali,Shah Masood Ahmad,Xiaojian Li,Lei Yang,Yaoguang Wang,Huan Wang,Qin Wei
A novel label-free electrochemiluminescent (ECL) immunosensor based upon luminol functionalized platinum nanoparticles loaded on graphene sheets (Lu-Pt@GS) as sensing platform was fabricated for highly sensitive and selective determination of prostate specific antigen (PSA). In this work, for the first time luminol was employed as both ECL luminescence reagent and reductants to in-situ reduce H 2 PtCl 6 forming Pt NPs on surface of GS. A great deal of luminol could be attached onto the surface of Pt NPs within the reduction process, which can generate strong ECL emission. Pt NPs not only could enhance ECL signals of luminol but supply active sites for the immobilization of PSA antibodies with micro friendly environment. For preventing the consecutive reaction among luminol and H 2 O 2 , single-step cycle pulse was adopted, resulting in stable and strong ECL signals. Under optimized experimental conditions, the proposed ECL immunosensor acquired a wide linear range of 1 pg/mL to 10 ng/mL with a relatively low detection limit of 0.3 pg/mL for PSA. Furthermore, due to high sensitivity, simplicity and cost-effectiveness, the designed immunosensor provides a new method for detecting other important biomarkers in clinical analysis.
研究领域:
生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Long Wu,Shuhong Zhou,Yonghuan Yun,Lin Zhu,Bei Li,Weimin Zhang
Based on the exchange of the DNA duplex-quadruplex, a multifunctional Pb(II) ion probe was fabricated, in which Pb(II) ion-driven DNA duplexes act as a bridge to link CdSe/ZnS-luminol and Fe 3 O 4 nanoparticles . As Pb(II) ion owns a strong binding capacity for G-quadruplex (G4), it can unwind the DNA duplex and release its complementary strand. In this strategy, three detection modes, chemiluminescence (CL), fluorescence (FL) assay and colorimetric assay (CA), can be realized via DNA duplex bridged CdSe/ZnS and Fe 3 O 4 nanocomposites . Amongst, FL assay shows the best detection performance, and it exhibits a linear range of 10 nM to 10 µM, as well as the limit of detection (LOD) with 1.63 nM (n = 3, ± 3.5%). In the selective analysis, Pb(II) ion can be specifically detected in the nine different interfering metal ions . Besides, the magnetic probe requires only one “mixing and separation” operation and greatly improves the detection efficiency. The three assays can be combined as one method to achieve a simple and rapid feature. Such a three-in-one analytical platform, therefore, can serve as a powerful tool for selective, accurate and sensitive detection of Pb(II) ion.
研究领域:
生物化学
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分析化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2025
·
IF 5.1
Jiyang Liu,Hongzhan Liu,Yutong Liu,Hao Jiang,Morteza Hosseini,Cun Qi Wu,Guobao Xu
The unique redox transition between Mn (III)-porphyrin and Mn(IV)-porphyrin centers confers exceptional catalytic properties to Mn-porphyrinic frameworks. In this study, we synthesized Mn-based metal-organic frameworks (Mn-MOFs) via a facile one-pot method using manganese (II) chloride tetrahydrate and 5,10,15,20-Tetrakis(4-carboxyphenyl)porphyrin (TCPP) as the ligand. The resulting Mn-MOFs exhibit unprecedented peroxidase-mimetic activity, enhancing luminol/H2O2 chemiluminescence (CL) by over 1200-fold. Capitalizing on the selective quenching effect of dopamine on the Mn-MOFs/luminol/H2O2 system, we developed a rapid CL analytical strategy with a linear detection range of 5–1000 nM and a detection limit of 3.95 nM (S/N = 3). Furthermore, this method was successfully applied for the quantitative determination of dopamine in serum samples, achieving a recovery rate of 98.3–104.4%. These findings highlight the potential of Mn-porphyrin-based MOFs as efficient catalysts in CL detection platforms, paving the way for their broader application in bioanalytical sensing.
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Shuting Cheng,Rui Xu,Fengzhen Yang,Jingcheng Huang,Xia Sun,Xue Huang,He Li,Falan Li,Yemin Guo,Mohammad Hasanzadeh,Yelong Zhu
A novel sandwich electrochemiluminescence (ECL) aptasensor was developed for highly sensitive detection of kanamycin using luminol-functionalized aptamer as a signal probe. The aptasensor used polyethyleneimine (PAMAM), molybdenum disulfide, and multi-walled carbon nanotubes as the substrate, which provided enough binding sites for aptamer1 (the aptamer which modified NH 2 ) coupling. We found that kanamycin could be detected using the aptamer1 containing the same base sequence as aptamer2 (the aptamer which modified SH) on the electrode self-assembly. In addition, PAMAM nanocomposites can be used to effectively improve the ECL intensity by loading a high volume of luminol molecules and silver nanoparticles. In the presence of kanamycin, the sandwiched aptasensor was formed between aptamer1 and the probe of aptamer2 connecting silver nanoparticles, luminol, and PAMAM, resulting in a proportional increase of ECL intensity. Since the significantly enhanced loading of luminol by PAMAM accelerated the electron transfer, the sensitive aptasensor exhibited a wide linear range of detection from 1 × 10 -3 to 1 × 10 3 ng/mL and a low detection limit of 0.21 pg/mL (S/N) for kanamycin. The fabricated aptasensor was successfully applied in quantitative analysis of kanamycin in milk samples.
研究领域:
分析化学
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电化学
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生物传感器
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食品安全
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Xue Fan,Lujie Wang,Hongxin Wang,Liuyi Huang,Jiahui Lin,Xia Gao,Fengna Xi
An immunosensing platform was developed based on the enhanced electrochemiluminescence (ECL) of luminol-dissolved oxygen (O 2 ) by nanochannel-confined Ni(OH) 2 -CeO 2 composite nanozyme, which is able to sensitively detect cytokine. The mesoporous silica nanochannel array film (SNF), decorated on a cost-effective ITO electrode, features ultrasmall (2∼3 nm) nanochannels, enabling the confinement of in situ synthesized Ni(OH) 2 -CeO 2 composite nanozyme via a continuous electrodeposition process. Ni(OH) 2 -CeO 2 exhibits dual peroxidase (POD) and oxidase (OXD) enzyme-like activities, serving as an efficient oxygen reduction reaction (ORR) catalyst to produce reactive oxygen species (ROS) and catalyze luminol oxidation. Compared to nanozymes synthesized on flat electrodes, nanochannel-confined nanozyme demonstrates superior ECL enhancement. The combination of Ni(OH) 2 and CeO 2 exhibits strong synergistic catalytic performance, boosting ECL of luminol-O 2 under neutral conditions by 33.7 orders compared to electrode without confined nanozyme. Using tumor necrosis factor-alpha (TNF-α) as a proof-of-concept demonstration, immunosensor is fabricated by immobilization recognition antibodies on the SNF outer surface. TNF-α binding induces immunocomplex formation, reducing ECL signal by increasing interfacial resistance and hindering luminol diffuse. This enables sensitive TNF-α detection over a wide linear range (10 fg/mL to 10 ng/mL) with an ultralow detection limit of 8 fg/mL. The immunosensor demonstrates good selectivity, stability, and reproducibility.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Waner Hou,Xun Lei,Zhimin Liu,Xuanxuan Hao,Jintao Cheng,Yaxin Li,Leqian Hu,Peng Li
Herein, ZnCo bimetallic zeolite imidazole framework (ZnCo-ZIF) nanocubes were synthesized and used as 2e − oxygen reduction reaction (2e-ORR) electrocatalysts. ZnCo-ZIFs showed excellent ORR catalytic activity, which was benefit for producing reactive oxygen species (ROS) efficiently and enhancing the electrochemiluminescence (ECL) emission from luminol/H 2 O 2 system. The reaction mechanism investigation implied that ORR reaction could produce ROS, and the ECL response exhibited a positive correlation with the catalytic activity of ZnCo-ZIFs promoter. In addition, the electrocatalytic activity of different Zn/Co molar ratio (1/ 9, 1/5, 1 /1, 5/1 and 9/1) were investigated, the results showed that ZnCo-ZIF-IV with Zn/Co molar ratio of 5/1 displayed the highest ORR electrocatalytic performance, and the onset potential, H 2 O 2 production rate, the faraday efficiency were 0.67 V (vs. RHE), 67.17 % and 50.74 %, respectively. Finally, an ECL immunosensor based on the promoting effect of ZnCo-ZIF-IV nanocubes on the luminol/H 2 O 2 ECL system was proposed for the sensitive zearalenone assay. This strategy offers a new way to study the luminol-cathodic ECL system.
研究领域:
电化学
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纳米材料
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催化化学
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分析化学
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生物传感器
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-11
·
IF 5.3
Li Ruidan,Tian Li,Song Yujia,Guo Yanjia,Ma Guangping,Han Pengfei,Jiang Hanyue,Wang Wenzhuo,Lu Juan
The synthesis of self-supporting material ZnMnO₂ by electrospinning and high-temperature calcination is reported. A highly sensitive electrochemiluminescence (ECL) sensor for Amaranth Red (AR) detection was constructed using Luminol@Ti₃AlC₂ and ZnMnO₂ with multi-step signal amplification. Luminol-O 2 served as the ECL probe with luminescent signal originating from its reaction with dissolved oxygen. Ti₃AlC₂ was introduced as the carrier to enhance the effective concentration of the ECL reagent and improve the signal. ZnMnO 2 as the co-reaction promoter could further improve the ECL value. The highest signal was attributed to the combined catalytic action of Luminol@Ti 3 AlC 2 and ZnMnO 2 . A new method for the detection of AR was developed due to the resonance energy transfer (RET) between Luminol and AR. Under optimal conditions, the response to AR exhibited a linear range from 5.0 × 10 −15 to 5.0 × 10 −5 mol·L −1 . The sensor achieved satisfactory AR detection in candy samples. Graphical
研究领域:
分析化学
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材料科学
·
电化学
·
传感器技术
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）