ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Yuqiang Xiang,Huaduo Yan,Bingjie Zheng,Aroosha Faheem,Yonggang Hu
High-throughput analyses of multitarget markers can facilitate rapid and accurate clinical diagnosis. Suspension array assays, a flow cytometry-based analysis technology, are among some of the most promising multicomponent analysis methods for clinical diagnostics and research purposes. These assays are appropriate for examining low-volume, complex samples having trace amounts of analytes due to superior elimination of background. Physical shape is an important and promising code system, which uses a set of visually distinct patterns to identify different assay particles. Here, we presented a morphology recognizable suspension arrays based on the microorganisms with different morphologies. In this study, UiO-66-NH2 (UiO stands for University of Oslo) metal–organic frameworks (MOFs), was wrapped on the microorganism surface to form an innovative class of microorganism@UiO-66-NH2 composites for suspension array assays. The use of microorganisms endowed composites barcoding ability with their different morphology and size. Meanwhile, the UiO-66-NH2 provided a stable rigid shell, large specific surface area, and metal(IV) ions with multiple binding sites, which could simplify the protein immobilization procedure and enhance detection sensitivity. With this method, simultaneous detection of three colorectal cancer-related microRNA (miRNA), including miRNA-21, miRNA-17, and miRNA-182, could be easily achieved with femtomolar sensitivity by using a commercial flow cytometer. The synergy between microorganisms and MOFs make the composites a prospective barcoding candidate with excellent characteristics for multicomponent analysis, offering great potential for the development of high throughput and accurate diagnostics.
研究领域:
生物医学工程
·
分子生物学
·
癌症研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2020
·
IF 7.333
Xin-Xin Peng,Tongtong Guo,Hao Lu,Linlin Yue,You Li,Dan Jin,Guo-Jun Zhang,Fan Yang
MicroRNA (MiRNA)-based noninvasive diagnostics are hampered by the challenge in the quantification of circulating miRNAs using a general strategy. Here, we present a base-stacking effect-mediated ultrasensitive electrochemical miRNA sensor (BSee-miR) with a universal sandwich configuration. In the BSee-miR, a short DNA probe (10 nucleotides) self-assembled on a gold electrode surface could effectively capture the target miRNA synergizing with another sequence based on coaxial sandwich base-stacking, which rivals the fully complementary strength. Importantly, such a sandwich structure is flexible to incorporate signal amplification strategies (e.g., biotin–avidin) that are usually difficult to achieve in short sequence detection. Using this design, the BSee-miR achieves a broad dynamic range with a detection limit down to 7.5 fM. Furthermore, we found a high-curvature nanostructuring synergetic base-stacking effect that could improve the sensitivity of the BSee-miR by two orders of magnitude (79.3 aM). Our BSee-miR also has a single-base resolution to discriminate the highly homologous miRNAs. More importantly, this approach is universal and has been used to probe target miRNAs varying in sequences and secondary structures. Our ultrasensitive sensor could detect miRNA in cell lysates and human blood and distinguish cancer patients from normal individuals, promising a versatile tool to measure clinically relevant miRNAs for tumor diagnostics.
研究领域:
生物医学工程
·
分子生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Xiao-Mei Yan,Yin-Qi Wang,Yao Chen,Zeng-Ping Chen,Ru-Qin Yu
A simple but effective method for the detection of miRNAs was proposed by integrating exonuclease-III assisted target recycling amplification and repeated-fishing strategy. In the proposed method, exonuclease-III assisted target recycling amplification reaction is adopted to produce a large amount of DNA fragments with fluorescence group at its 5′ end in the presence of the target miRNA, which are then repeatedly fished out from the reaction mixture by a gold foil modified with a capture probe and transferred into a so-called ‘product tube’. The amount of the target miRNA can then be determined from the fluorescence measurement of the solution in the ‘product tube’. Application to the detection of miRNA-155 in samples of KH-2 and BRSA-2B cells revealed that the proposed method could achieve sensitive and accurate quantification of the target miRNA with a limit of detection of 36 fM and recovery rates in the range from 96.2% to 105%. Its simplicity, sensitivity and resistance to possible fluorescence interferences in complex biological samples make the proposed method a potentially competitive alternative for miRNAs detection in complex biological samples.
研究领域:
分子生物学
·
生物化学
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Keqin Deng,Xinyan Liu,Chunxiang Li,Haowen Huang
The loading capacity of thionin (Thi) on shortened multi-walled carbon nanotubes (S-MWCNTs) and acidified multi-walled carbon nanotubes (A-MWCNTs) was compared. Two DNA probe fragments were designed for hybridization with microRNA-21 (miR-21), the microRNAs (miRNAs) model analyte. DNA probe 1 (P1) was assembled on Au nanoparticles (AuNPs) modified electrode . MiR-21 was captured by the pre-immobilized P1. A signal nanoprobe was synthesized by loading large amount of Thi on S-MWCNTs with covalently bonded probe 2 (P2). Owing to the large effective surface area of MWCNTs , fast electron shuttle of MWCNTs, high-loaded Thi on S-MWCNTs, and the increased conductivity from AuNPs, after signal probe hybridized with miR-21, it gave rise to a magnified current response on electrode. The increased electrochemical current enabled us to quantitatively detect miR-21. Expensive bioreagents and labeled target/detection DNA or miRNAs were avoided in this strategy. The operation complexity and assay cost were also reduced.
研究领域:
分析化学
·
生物传感器与生物电子学
·
纳米技术
·
分子诊断
·
电化学
✓ 已验证
DOI
高影响因子
DEVELOPMENTAL CELL
·
2025
·
IF 8.7
Kun Shen,Mengxue Qu,Xuemei Zhou,Yingying Guo,Yicheng Zhong,Meng-xiang Sun,Peng Zhao
Fertilization of the egg cell by more than one sperm cell results in aneuploidy and genomic dysfunction. To avoid this, flowering plants have intricate polytubey block mechanisms that prevent additional pollen tubes from entering the ovule. In one mechanism for blocking polytubey, the fertilized egg cell secretes the aspartic endopeptidases EGG CELL-SPECIFIC1 (ECS1) and ECS2, which degrade pollen tube attractants. However, the mechanism underlying the egg-cell-specific expression of ECS1 and ECS2 was unknown. Here, we demonstrate that in Arabidopsis thaliana egg cells, SMALL RNA DEGRADING NUCLEASE (SDN) degrades microRNAs (miRNAs) targeting ECS genes, thereby allowing ECS1 and ECS2 to accumulate in egg cells to establish the polytubey block. Like Arabidopsis ecs1 ecs2 mutants, sdn1 sdn2 sdn3 mutants exhibited polytubey, as did plants specifically overexpressing miRNAs targeting ECS genes in egg cells. Therefore, the SDN-miRNA-ECS module allows ECS accumulation in egg cells to block polytubey.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale Advances
·
2025
·
IF 4.6
Ruiqi Chen,Bin Qiu,Chen Chen,Mingyuan Chen
Research has shown that miRNA-21 levels increase during vascular aging, making it a promising biomarker for vascular aging. Therefore, developing sensitive miRNA-21 detection techniques is crucial for early diagnosis, therapeutic intervention, and prognosis assessment of vascular aging. This work developed an enzyme-free fluorescence sensing platform based on upconversion nanoparticles (UCNPs) and a catalytic hairpin assembly (CHA), which uses 808 nm light-controlled switches and fluorescence resonance energy transfer (FRET) signal regulation for highly sensitive and specific quantification of miRNA-21. First, the loaded DNA hairpins and the UCNPs were released under weakly acidic conditions due to the disintegration of the organic framework material (ZIF-8). Under 808 nm irradiation, when the analyte miRNA-21 is present, the DNA hairpin (H1) undergoes specific unwinding, triggering CHA, leading to signal recovery of the fluorescent group on another DNA hairpin (H2) and quantitative detection of miRNA-21. The degree of fluorescence restoration of the sensor exhibited a good linear correlation with the miRNA-21 concentration in the range of 0.5–25 nM, and the limit of detection reached 0.128 nM. Moreover, this method exhibits excellent analytical performance in the detection of serum samples, with spiked recovery rates ranging from 98.2% to 102.8%. These data demonstrate that the light-controlled sensing platform has significant merits, such as high detection sensitivity, strong specificity, and minimal background interference, providing a reliable method for the rapid quantification of miRNA-21 in complex matrices.
研究领域:
生物传感器
·
纳米生物技术
·
分子诊断
·
微小RNA检测
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 23
·5779-5787
·
IF 3.637
Dai Panpan,Ke Jiajun,Xie Chenggen,Wei Liyun,Zhang Ying,He Yong,Chen Lijuan,Jin Juncheng
A sensitive electrochemiluminescence (ECL) assay for microRNAs (miRNAs) based on a semiconductor nanomaterial sensitized with noble-metal Au nanoparticles (NPs) is successfully developed. TiO 2 nanotubes (NTs) were equipped with Au NPs to obtain an enhanced ECL emitter. Then, an ECL assay for miRNA-21 was fabricated, which was based on the use of probe 2 DNA–functionalized Pt/PAMAM nanocomposites (NCs) assembled on the surface of Au/TiO 2 NT conjugate via DNA hybridization between probe 1 DNA and capture DNA. The Pt/PAMAM NCs act as an ECL quencher of Au/TiO 2 NTs via resonance energy transfer. After the binding of target miRNA-21 and the capture DNA, the Pt/PAMAM NCs were released and the ECL signal was recovered. An “off–on” ECL assay was achieved with a linear response from 0.01 to 10,000 pM. Finally, this method has been validated to be sensitive and specific for miRNAs in human serum samples. The ECL enhancement strategy opens a new way for fabricating various sensitive biosensors.
A sensitive “off–on” electrochemiluminescence analysis method was developed, which combined Au NP–enhanced ECL emission of TiO 2 nanotubes and an efficient energy-transfer system between Au/TiO 2 nanotubes and Pt/PAMAM nanocomposites
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Zhipeng Shen,Liyong He,Wenhai Wang,Lei Tan,Ning Gan
There are critical interests in the detection of microRNA (miRNA) because it can be a blood-borne biomarker, but analytical strategies are still limited by its small size, high sequence homology among family members and low abundance. In this work, three-dimensional magnetic DNA nanospheres were synthesized and immobilized on a gold stir-bar as encoded probes for miRNA capture and signal amplification . Electrochemical tags-labeled DNAs were immobilized on gold coated magnetic nanospheres via a hyperbranched hybridization chain reaction (HHCR). Subsequently, the magnetic DNA nanospheres were immobilized on the gold stir-bar as encoded probes. Target miRNAs were captured on the surface of the stir-bar by replacing the magnetic DNA nanospheres-encoded probes, and the probes were magnetically enriched for highly sensitive and selective electrochemical detection . The gold stir-bar assisted magnetic DNA nanospheres-encoded probes possess dual functions: They are as a nanocarrier to increase the loading amounts of HHCR products, and they are also a platform for efficient electrochemical signal amplification via magnetic enrichment. The method was successfully applied for the detection of miRNA21 and miRNA155 in a wide linear range of 5 fM to 2 nM, and with detection limits of 1.5 fM and 1.8 fM, respectively. The preliminary application of the method suggests that it has great potential in the detection of miRNAs in serum samples.
研究领域:
分析化学
·
纳米技术
·
生物标志物研究
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Ye Tian,Xinxi Li,Jingdong Tang,Lei Zhang,Shuai Jiang,Halimulati Muertizha,Zhenwei Yang
Diabetic foot ulcer (DFU), a severe complication of diabetes, necessitates sensitive detection of pathogenic microRNAs (miRNAs) as emerging diagnostic biomarkers. The exponential amplification reaction (EXPAR) offers high efficiency and rapid kinetics for miRNA detection, but its application is limited by persistent nonspecific amplification arising from spurious template–template interactions and interference from partially complementary sequences in complex samples. To address this, we present an integrated strategy combining a blocking probe prehybridized to the EXPAR template to sterically hinder nonspecific hybridization, magnetic bead-based isolation of target-bound probes to remove interferents, and a self-priming mechanism that regenerates the EXPAR primer only upon target recognition. This design ensures high specificity and minimizes primer-independent amplification. Coupling this optimized EXPAR platform with a G-quadruplex-based signal transduction system enables facile colorimetric readout for instrument-free visual detection. The method achieves a detection limit of 41.4 aM across a dynamic range of 100 aM to 50 pM for DFU-associated miRNAs, with excellent selectivity against nontarget miRNAs and consistent reproducibility in fibroblasts and serum. This work provides a generalizable solution to the specificity issue in EXPAR and delivers a reliable, sensitive platform for miRNA profiling in clinical and point-of-care settings.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2020
·
IF 4.008
Sulin Lou,Xiulan Zhu,Zhiyong Zeng,Huina Wang,Bin Jia,Hui Li,Zhangli Hu
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play a crucial role in post-transcriptional gene regulation not only in animals and land plants, but also in microalgae . Among all the microalga, Dunaliella salina ( D. salina ) has the highest content of beta-carotene, and a draft genome of this species was recently published. Thus far, few D. salina miRNAs have been identified. In this study, deep sequencing was performed to identify miRNAs in wild-type D. salina (Control) and in a high light and high salinity treatment group (HLS). Three small RNA (sRNA) libraries were constructed for Control and HLS, and an average of 11,542,169 and 16,877,598 clean tags were obtained, respectively. The sRNA length distribution showed that 21 nt sRNAs were the most abundant class in both Control and HLS, which is consistent with the sRNA distribution of model organism Chlamydomonas reinhardtii . Eighty-four known miRNAs and 1054 novel miRNAs were found in Control. Among them, nine known miRNAs and 39 novel miRNAs had altered levels in response to HLS treatment. The expression differences of selected miRNAs and their target genes in Control and HLS were verified by qRT-PCR, which showed that novel-m0533-3p was up-regulated and its predicted target malate dehydrogenase encoded gene was clearly down-regulated in HLS at both transcription and the protein level. Moreover, some key carotenoid pathway genes, such as PSY , PDS and LCYB were all up-regulated in HLS. These findings are providing a new insights into a miRNA-mediated beta-carotene accumulation mechanism in D. salina .
研究领域:
植物学
·
分子生物学
·
基因组学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Qian Zhao,Jiafang Piao,Weipan Peng,Yang Wang,Bo Zhang,Xiaoqun Gong,Jin Chang
Identifying the microRNA (miRNA) expression level can provide critical information for early diagnosis of cancers or monitoring the cancer therapeutic efficacy. This paper focused on a kind of gold-nanoparticle-coated polystyrene microbeads (PS@Au microspheres)-based DNA probe as miRNA capture and duplex-specific nuclease (DSN) signal amplification platform based on an RGB value readout for detection of miRNAs. In virtue of the outstanding selectivity and simple experimental operation, 5′-fluorochrome-labeled molecular beacons (MBs) were immobilized on PS@Au microspheres via their 3′-thiol, in the wake of the fluorescence quenching by nanoparticle surface energy transfer (NSET). Target miRNAs were captured by the PS@Au microspheres-based DNA probe through DNA/RNA hybridization. DSN enzyme subsequently selectively cleaved the DNA to recycle the target miRNA and release of fluorophores, thereby triggering the signal amplification with more free fluorophores. The RGB value measurement enabled a detection limit of 50 fM, almost 4 orders of magnitude lower than PS@Au microspheres-based DNA probe detection without DSN. Meanwhile, by different encoding of dyes, miRNA-21 and miRNA-10b were simultaneously detected in the same sample. Considering the ability for quantitation, high sensitivity, and convenient merits, the PS@Au microspheres-based DNA probe and DSN signal amplification platform supplied valuable information for early diagnosis of cancers.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 22
·4991-4999
·
IF 5.227
Jinyue Shi,Yu Lin,Weiling Qin,Mingxiang Li,Yuyi Zhou,Yeyu Wu,Hu Luo,Ke-Jing Huang,Xuecai Tan
Graphdiyne (GDY) is an sp and sp2 co-hydrocarbon allotrope whose particular structure endows it with many fascinating properties, including abundant chemical bonds, high conjugation, natural pores, high carrier mobility, high conductivity and stability, etc. In this work, two-dimensional graphdiyne is prepared as an electrode substrate material coupling with an exonuclease III-assisted amplification strategy to construct a superior-performance self-powered biosensor based on enzymatic biofuel cells for highly sensitive detection of the tumour marker miRNA-21. Glucose oxidase (GOD) is first immobilized on the GDY/AuNP composite to prepare a bioconjugate. GDY/AuNP modified carbon cloth is used as an enzyme biofuel cell electrode, which is then modified with bilirubin oxidase as a biocathode. The bioconjugate binds to GOD through specific binding to the bioanode. When miRNA-21 is present, specific recognition by exonuclease III in the system results in cleavage of the capture probe, and miRNA-21 is recovered and involved in the cycle. The target miRNA-21 then causes corresponding changes in the open-circuit voltage of the self-powered system. Based on this, a sensitive detection method was constructed, within the scope from 0.1 fM to 0.1 nM with a shallow detection limit of 55.2 aM (S/N = 3). The new approach triumphantly has been used to detect miRNA-21 in serum, which provides a compelling new way for early diagnosis of related cancers.
研究领域:
生物传感器
·
纳米材料
·
癌症诊断
·
生物化学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）