Journal of Environmental Sciences
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2025
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IF 6.3
Qiao Xu,Na Liu,Jianjian Wu,Fei Ge
The coexistence of heavy metals and antibiotics in agricultural soil poses a critical challenge to crop safety. However, the molecular mechanisms underlying their combined toxic effects in real multi-pollutant environments remain poorly understood. This study elucidated the genotoxic mechanisms underlying the effects of cadmium (Cd) and oxytetracycline (OTC) on rice ( Oryza sativa L.) in the presence of copper (Cu). Results indicated that ambient Cu (50 mg/kg) significantly reduced Cd and OTC accumulation in rice roots by 86.6 % and 68.9 %, respectively. Density functional theory calculations further confirmed that this reduction stemmed from the formation of stable OTC-Cd-Cu ternary complexes. Despite the reduced uptake of Cd and OTC, Cu unexpectedly enhanced genotoxicity. Specifically, compared to the OTC-Cd group, the OTC-Cd-Cu group induced more DNA strand breaks. Further mechanistic investigations showed that Cu induced downregulation of the cell cycle regulators CYCB1 (by 20 %) and WEE1 (by 17.9 %), resulting in inhibition of G2/M transition and impaired DNA repair. Molecular dynamics simulations revealed that Cu enhanced the binding energy between DNA and OTC-Cd (-4178.17 kJ/mol), thereby destabilizing the DNA structure, as evidenced by circular dichroism analysis. Furthermore, thermodynamic parameter analysis suggested that the interaction between DNA and pollutants was an exothermic reaction. Importantly, the OTC-Cd-Cu ternary system exhibited a higher degree of impairment to DNA stability than the OTC-Cd binary system. These findings provide critical insights into the molecular basis of crop toxicity in agricultural soil and underline the urgency of comprehensive risk assessment of combined pollutants.
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ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Hanwen Li,Yonghao Wang,Lingquan Zeng,Chuyun Jin,Peng Zhang,Fenglian Qi,Caifeng Ding
Precision chromatic sensing remains a critical challenge for environmental diagnostics due to limited color resolution in field-deployable platforms. Herein, we present an aptamer-engineered trichromatic colloidal crystal hydrogel (ATCCH) biosensor that integrates triple colloidal crystal arrays with oxytetracycline (OTC)-specific molecular recognition. This system combines programmable stepwise vertical deposition of SiO2 colloids with the formation of OTC-specific aptamers anchored through gold nanoparticle (AuNP) bridges, creating nanoconfined sensing cavities for target-triggered photonic band gap modulation. OTC binding triggers aptamer structural transitions via π–π stacking and hydrogen bonding, disrupting AuNP-mediated cross-linking and inducing synergistic lattice expansion across three photonic domains. This generates independent wavelength shifts (R: 867 → 921 nm, G: 723 → 771 nm, B: 548 → 601 nm) with cooperative naked-eye-discernible color transitions (R: pale red → red, G: indigo → brick yellow, B: purple → blue) as the concentrations of OTC increased from 0.1 to 100 μg/mL. The three-dimensional nanoconfinement architecture enables ultrastable operation in high-ionic-strength environments (0.5 M NaCl), achieving a detection limit low to 33 ng/mL via reflection spectroscopy (0.1–80 μg/mL log–linear range, R2 = 0.966:0.956:0.992 for R/G/B domains) and field-applicable chromatic thresholds (0.5 μg/mL) matching aquaculture safety standards. The triple-signal synergy enhances color resolution by at least 2-fold compared with monochromatic systems. This multichromatic photonic-aptamer nanoengineering strategy establishes a new paradigm for field-deployable environmental sensors that bridge laboratory-grade quantification with instant visual readouts.
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Journal of the Taiwan Institute of Chemical Engineers
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2026
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IF 6.9
Lejing Zhou,Haojun Ji,Xiaobo Gong,Yiqing Liu,Yong Liu,Junfeng Zhao
Background Antibiotic pollution of water bodies is a major problem to be solved in the current environmental field. Methods In this study, copper modified biochar (CuBC) catalyst was successfully prepared using fig branch as biochar source, and an efficient catalytic oxidation system was constructed for the degradation of oxytetracycline (OTC). Significant findings The obtained CuBC catalyst had a porous structure with irregular surface morphology. The multi valence states of Cu + and Cu 2+ in CuBC were beneficial for peroxymonosulfate (PMS) activation. The removal of OTC reached 91.38% in CuBC/PMS system within 45 min. The CuBC catalyst exhibited excellent stability and catalytic activity over a wide pH range of 3 to 11. The efficient degradation of OTC was due to the synergistic effect of free radical (·OH and SO 4 ·- ) and non-free radical ( 1 O 2 ) pathways in CuBC/PMS system. Furthermore, the degradation pathways were proposed and most intermediates during the degradation process of OTC have reduced toxicity in CuBC/PMS system. This research provides a promising strategy for resource utilization of biomass and antibiotic pollution control.
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JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 10.6
Yetao Zeng,Xiaojuan Xu,Jiayin Zhang,Dazuo Yang,Jiamei Pan,Haiqing Wang
P. aibuhitensis presence was associated with lower sediment OM (0.94%) and TP (19.73 mg/kg). • OTC–TWP exposure was associated with higher sediment OM (1.74%) in polychaete-containing systems. • OTC–TWP exposure was associated with lower Chao1 (3947.49) and ACE (3982.72) indices. • OTC–TWP exposure altered T-SOD (52.16 U/mg) and AChE (40.41 U/mg) activities in P. aibuhitensis. Tyre wear particles (TWPs) and antibiotics are co-occurring contaminants in coastal sediments, yet their combined effects on polychaete-associated sediment processes remain under reported. This study provides new evidence on the joint effects of TWPs and oxytetracycline (OTC) on a polychaete–sediment ecosystem. Polychaete–sediment systems were exposed to TWPs (1% of sediment dry weight), OTC (2.0 mg·L −1 ), and their combined treatment for 16 days. In polychaete-containing sediments, OTC–TWP co-exposure was associated with higher OM (1.74%) relative to the polychaete group, consistent with a reduced sediment-reworking effect. Co-exposure also altered inorganic nitrogen profiles and increased alkaline phosphatase activity to 184.37 nmol·g −1 . Microbial analysis showed that OTC–TWP co-exposure was associated with lower Chao1 and ACE indices, and Desulfobacterota showed a higher relative abundance in TWP groups. OTC and TWP co-exposure triggered oxidative stress and physiological alterations in P. aibuhitensis , reflected by increased MDA levels and changes in antioxidant responses. Moreover, exposure to these pollutants resulted in the suppression of AChE and LZM activities. Given the global occurrence of TWPs and antibiotics in coastal environments, this study provides insights into the ecological risks of emerging contaminant mixtures and highlights the need to incorporate particle–antibiotic co-pollution into sediment risk assessment. Download: Download high-res image (212KB) Download: Download full-size image
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Xingbo Ren,Wei Zhang,Yu Wang,Yulong Xu,Yue Wang,Xuwei Chen
Tetracycline residues have raised widespread concern, among which oxytetracycline (OTC) has attracted particular attention because of its potential risks to human health. A sensitive analytical method for detecting tetracycline residues in complex samples is thus needed. Herein, a novel fluorescent sensing platform based on an europium(III) (Eu 3+ )-functionalized covalent organic frameworks (TpBy-COF) for the selective detection of OTC is developed. The fluorescence of TpBy-COF@Eu is hardly observed due to the non-radiative decay from coordinated water molecules. However, the fluorescence signals of TpBy-COF@Eu-based sensing platform is significantly enhanced with the introduction of OTC, attributed to the specific displacement of coordinated water molecules by OTC, which acts as an effective antenna ligand by transferring energy to the excited states of Eu 3+ ,thereby sensitizing its characteristic red emission. Moreover, this TpBy-COF@Eu-based sensing platform exhibits the favorable sensitivity and selectivity for OTC monitoring with a low detection limit as low as 0.12 μmol/L. The TpBy-COF@Eu-based sensing platform is successfully validated in real samples, demonstrating the practical potential for OTC monitoring in complex matrices.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Hongxu Chen,Xingyu Ma,Qi Xu,Jialing Yu,Shoufeng Zhang,Rongrong Yuan
Green synthesis: Mechanochemical synthesis of porphyrin COF with tunable crystallinity, low cost. • Ultra-trace detection: OTC detection limit 0.0138 ng·mL −1 , linear range 0.001–1 ng·mL −1 . • High stability & selectivity: Resists 100× interferences; >92% signal retention after 10 days. • Real-world reliable: River water spike recovery 97–103%, RSD < 3.4%. This study devised a green and cost-effective mechanical grinding approach to successfully synthesize porphyrin-based covalent organic framework (COF) materials with adjustable crystallinity and specific surface area under near-solvent-free conditions. 5,10,15,20-tetra(4-aminophenyl)porphyrin (TAPP) and 2,5-dihydroxyterephthalaldehyde (DTA) were employed as monomers. Through the optimization of experimental conditions, precise control over the ordered structure and pore characteristics of the materials was attained, leading to the formation of a crystalline COF with a high specific surface area and abundant pores. The electrochemical aptamer sensor constructed based on this optimized material demonstrated remarkable detection performance for oxytetracycline. The sensor response current exhibited a favorable linear relationship with the oxytetracycline concentration, characterized by a low detection limit, high selectivity, and excellent stability and reproducibility. In the spiked recovery experiments of actual samples, this sensor also showed extremely high reliability. This research not only offers an efficient sensing platform for the rapid detection of trace oxytetracycline but also paves a new way for the rational design of green-synthesized functional COF materials and their application in the field of electrochemical sensing.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Yunqing Li,Xiaoying Zheng,Haidong He,Ruijie Hu,Zongshuo Han,Jiaqing Tao,Tao Lin,Wei Chen
Oxytetracycline (OTC), an emerging “low-concentration, high-toxicity” contaminant, presents considerable hurdles to wastewater treatment processes. This study systematically evaluated for the first time the impacts of OTC on the operational performance, sludge characteristics, and microbial metabolic activity across three treatment systems: microalgal-bacterial granular sludge (MBGS), aerobic granular sludge (AGS), and activated sludge (AS). Results demonstrated that MBGS exhibited superior treatment efficiency, maintaining stable removal of 500 μg/L OTC at 88.06 ± 1.45 % ( p < 0.05). MBGS adapted to OTC exposure by increasing ATP content and reducing lactate dehydrogenase release. Acclimated MBGS primarily removed OTC through biodegradation. Moreover, multiple OTC transformation products with reduced toxic potential were detected, signifying that MBGS systems achieve efficient microbial degradation. Metagenomic analyses revealed that Pseudomonadota in MBGS displayed high adaptability under OTC exposure. Additionally, OTC exposure upregulated carbohydrate and energy metabolism in MBGS, thereby enhancing overall microbial metabolic activity. Alphaproteobacteria contributed most significantly to key functional genes, underscoring their critical role in contaminant removal in the MBGS. Redundancy analysis highlights a robust association between Alphaproteobacteria and the abundance of antibiotic resistance genes. This study confirms the MBGS's resilience to OTC-contaminated wastewater, highlighting its potential for efficient antibiotic wastewater treatment.
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Frontiers in Microbiology
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2022
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IF 6.064
Cao Xiaoxu,Zhao Wei,Zhang He,Lin Jitong,Hu Jingying,Lou Yanhong,Wang Hui,Yang Quangang,Pan Hong,Zhuge Yuping
Introduction: The large-scale development of animal husbandry and industrialization lead to more and more serious co-contamination from heavy metals and antibiotics in soils. Ecotoxic effects of residues from antibiotics and heavy metals are of increasing concern.Materials and Methods: In this study, oxytetracycline (OTC) and cadmium (Cd) were selected as target pollutants to evaluate the individual and combined effects on nitrification process using four different soil types sampled from North to South China through a 56-day incubation experiment.Results and Discussion: The results demonstrated that the contaminations of OTC and Cd, especially combined pollution had significant inhibitory effects on net nitrification rates (NNRs) as well as on AOA and AOB abundance. The toxic effects of contaminants were greatly enhanced with increasing OTC concentration. AOB was more sensitive than AOA to exogenous contaminants. And the interaction effects of OTC and Cd on ammonia oxidizers were mainly antagonistic. Furthermore, Cd contaminant (with or without OTC) had indirect effects on nitrification activity via inhibiting mineral N and AOA/AOB, while OTC alone indirectly inhibited nitrification activity by inhibiting ammonia oxidizers. The results could provide theoretical foundation for exploring the eco-environmental risks of antibiotics and heavy metals, as well as their toxic effects on nitrification processes.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2022
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IF 5.437
Min Cui,Songguo Yu,Yufei Yu,Xuehai Chen,Jia Li
Croplands have become a hotspot for antibiotic and microplastic (MP) pollution. However, little is known regarding their combined effects on crops. In this study, the individual and combined effects of oxytetracycline (OTC) and three MPs (i.e., polypropylene (PP), polyamide (PA), and polyvinylchloride (PVC)) on cherry radish were investigated using pot experiments. Individually, OTC (50 mg kg −1 ), PA (2%, w/w), and PP (2%, w/w) induced negligible effects on cherry radish biomass and the root/shoot ratio. However, PVC (2%, w/w) significantly inhibited cherry radish growth; that is, its shoot and root fresh weight decreased by 46.2% and 81.1%, respectively. In the combined exposure groups, OTC alleviated the adverse effects of PVC on the cherry radish leaf number and shoot fresh weight. This was linked to that OTC increased the content of photosynthetic pigments . Superoxide dismutase activity in cherry radish roots was inhibited to different extents in all treatment groups except for the PA and PVC treatments. Malondialdehyde (MDA) content in cherry radish roots increased in all treatment groups, suggesting that both OTC and MPs caused oxidative damage to cherry radish root cells, therefore inhibiting cherry radish root growth. However, the presence of OTC non-significantly changed the effects of MPs on cherry radish roots. Irrespective of OTC presence, MPs induced a reduction in the root/shoot ratio of cherry radish, suggesting that the inhibitory effect of MPs on cherry radish roots was stronger than that on shoots. These findings contribute to the evaluation of the phytotoxicity of antibiotics and MPs in soil-vegetable systems.
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ACS Applied Nano Materials
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2022
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IF 6.14
Lei Cao,Mengyan Zhou,Jiaxi Wang,Qiang Zhu,Tingting Liu,Shushu Ding,Ding-Yi Fu
Gold–silver alloy nanoclusters (AuAgNCs) were synthesized, protected by glutathione S-transferase (GST). The as-prepared GST-AuAgNCs displayed strong (QY = 15.9%) fluorescence with two emission peaks around 477 and 653 nm under excitation of 395 nm. In this system, both the NC core and the organic shell contributed to the development of a “turn-on” ratiometric fluorescent probe for sensitively and selectively detecting oxytetracycline (OTC). First, the green fluorescence at 477 nm enhanced with the addition of OTC into GST-AuAgNC solution, whereas the red fluorescence at 653 nm remained unchanged. This “turn-on” ratiometric fluorescence may stem from the aggregation-induced emission effect and the reduction of Ag(I). The fluorescence ratio of GST-AuAgNCs displayed good linearity when the concentration of OTC was in the range of 0∼50 μM, and the detection limit was achieved down to 32.66 nM. Second, benefiting from the strong interaction between GST and OTC, the proposed method exhibited good anti-interference toward OTC in comparison with the common ions and natural amino acids. Moreover, the variation of ratiometric fluorescence intensity resulted in the corresponding red-to-green fluorescent color change, which could be employed to establish a simple paper-based analytical method for OTC colorimetric detection. Based on the superiority of high sensitivity and selectivity, as well as good accuracy and biocompatibility, the GST-AuAgNCs were successfully applied for detecting OTC in cells.
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CHEMOSPHERE
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2022
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IF 8.943
Hao Zhang,Zuhan Ge,Yaguang Li,Suzhen Huang,Jibiao Zhang,Zheng Zheng
Oxytetracycline and sulfadiazine were widely used and they entered the environment through various channels such as domestic sewage, medical wastewater and agricultural wastewater, causing significant ecological risk. To determine the effects of different antibiotic concentrations on submerged macrophytes , Vallisneria natans was exposed to solutions containing different concentrations of oxytetracycline and sulfadiazine (0.1 mg/L、1 mg/L、10 mg/L、50 mg/L). After 20-days exposure, we found that 10 mg/L groups had a significant effect on Vallisneria natans . Under high antibiotic concentrations, the growth of Vallisneria natans was inhibited, chloroplasts were deformed, the chlorophyll content was reduced, and antioxidant enzyme activities , such as superoxide dismutase and glutathione , were increased. There was no significant difference between the control group and groups with low antibiotic concentrations (≤1 mg/L). The N-acyl- l -homoserine lactone concentration tended to increase with increasing antibiotic concentrations. The presence of antibiotics also affected the microbial community structure of biofilms on the submerged macrophytes. For example, the higher the concentration of antibiotics, the higher the proportion of Proteobacteria. These results suggest that high concentrations of oxytetracycline and sulfadiazine can disrupt homeostasis , induce effective Vallisneria natans defense mechanisms and alter biofilms in aquatic ecosystems .
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2021
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IF 3.764
Lianyu Jia,Yang Jin,Jun Li,Zhizhen Wei,Ming Chen,Jun Ma
In this work, a spiral microchannel reactor was used to study the photocatalytic degradation process of oxytetracycline (OTC) in a titanium dioxide (TiO2) slurry system. The influence of parameters such as residence time, TiO2 dosage, OTC concentration, light intensity, and concentration of added ions (NO3–, SO42–, Ca2+, Fe3+) on OTC removal was systematically investigated, and the degradation stability of OTC in the microreactor was evaluated. The results show that under the best experimental conditions, the degradation rate of OTC can reach 99.33%, the initial stage apparent reaction rate constant is 7.7124 min–1, and the space–time yield is 142.79 m3 pollutants/(m3 reactor day). In addition, the results prove the high efficiency and stability of OTC degradation in the microreactor. OTC can be efficiently degraded under high TiO2 dosage, high initial OTC concentration, and low light intensity, which demonstrates the outstanding advantages of spiral microchannels for OTC degradation in slurry systems. This study has a guiding significance for the efficient photocatalytic degradation of tetracycline antibiotics in slurry systems.
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