ENVIRONMENTAL TECHNOLOGY
·
2023
·
IF 2.8
Jiaying Zhu,Yanyang Wu,Bin Wu,Kui Chen,Lijun Ji
m-Cresol and p-cresol are widely used in medicine and pesticides as important chemical intermediates. They are generally produced as a mixture in industry and are difficult to separate due to the similarities in both chemical structures and physical properties. The adsorption behaviours of m-cresol and p-cresol on zeolites (NaZSM-5 and HZSM-5) with different Si/Al ratios have been compared by static experiments. Selectivity on NaZSM-5(Si/Al = 80) could be greater than 6.0. Adsorption kinetics and isotherms were investigated in detail. The kinetic data was correlated by PFO, PSO, and ID models, the NRMSE of which were 14.03%, 9.41%, and 21.11%, respectively. In the meanwhile, according to the NRMSE of Langmuir (6.01%), Freundlich (57.80%), D-R (1.1%), and Temkin (0.56%) isotherms, adsorption on NaZSM-5(Si/Al = 80) was mainly a monolayer and chemical adsorption process. It was endothermic for m-cresol and exothermic for p-cresol. The Gibbs free energy, entropy, and enthalpy were calculated accordingly. The adsorption of cresol isomers on NaZSM-5(Si/Al = 80) were both spontaneous, and it was exothermic (ΔH = −37.11kJ/mol) for p-cresol while endothermic (ΔH = 52.30kJ/mol) for m-cresol. Additionally, ΔS were respectively −0.05 and 0.20 kJ·mol−1·K−1for p-cresol and m-cresol, which were both close to zero. The adsorption was mainly driven by enthalpy. The result of breakthrough further demonstrated m-cresol and p-cresol could be separated effectively by NaZSM-5(Si/Al = 80). Additionally, the selectivity increased from 7.53 to 14.72 after four cycles regeneration with 9.95% and 53.96% decreases in the adsorption amounts for m-cresol and p-cresol, respectively. In conclusion, NaZSM-5(Si/Al = 80) could be a feasible adsorbent for the separation of m-cresol and p-cresol.
研究领域:
化学工程
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材料科学
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DOI
PubMed
高影响因子
Microbial Cell Factories
·
2017
·Vol. 16
·期 1
·1-10
·
IF 3.681
Zhang Hui,Li Mengya,Li Jie,Wang Guangli,Liu Yuan
Quizalofop- p -ethyl (QPE) is a post-emergence herbicide that effectively controls grass weeds and is often detected in the environment. However, the biochemical and molecular mechanisms of QPE degradation in the environment remains unclear. In this study, a highly effective QPE-degrading bacterial strain J-2 was isolated from acclimated activated sludge and identified as a Pseudomonas sp., containing the QPE breakdown metabolite quizalofop acid (QA) identified by Liquid Chromatography-Ion Trap-Mass Spectrometry (LC-IT-MS n ) analysis. A novel QPE hydrolase esterase-encoding gene qpeH was cloned from strain J-2 and functionally expressed in Escherichia coli BL21 (DE3). The specific activity of recombinant QpeH was 198.9 ± 2.7 U mg −1 for QPE with K
m
and K
cat
values of 41.3 ± 3.6 μM and 127.3 ± 4.5 s −1 . The optimal pH and temperature for the recombinant QpeH were 8.0 and 30 °C, respectively and the enzyme was activated by Ca 2+ , Cd 2+ , Li + , Fe 3+ and Co 2+ and inhibited by Ni 2+ , Fe 2+ , Ag + , DEPC, SDS, Tween 80, Triton X, β-mercaptoethanol, PMSF, and pCMB. In addition, the catalytic efficiency of QpeH toward different AOPP herbicides in descending order was as follows: fenoxaprop-P-ethyl > quizalofop-P-tefuryl > QPE > haloxyfop-P-methyl > cyhalofopbutyl > clodinafop-propargyl. On the basis of the phylogenetic analysis and multiple sequence alignment, the identified enzyme QpeH, was clustered with esterase family V, suggesting a new member of this family because of its low similarity of amino acid sequence with esterases reported previously.
研究领域:
环境科学
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分子生物学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuxin Wei,Sibo Chen,Hang Yan,Xiaoran Zhang,Xinyue Gao,Zhongli Cui,Yan Huang
A common aromatic and phenolic pollutant, p-nitrophenol (PNP), is widely used in various industry and has serious risk to the environmental health. Biosensors have been extensively employed as an alternative technology for pollutants monitoring. By mining the new sensing elements, more specific biosensors could be characterized for highly sensitive detection. Herein, the PnpR transcription factor was identified to activate the transcription of pnpC1 by binding to P pnpC1 promoter region in P. putida DLL-E4, and PNP was recognized as its specific inducer. The PnpR-based biosensor for detection of PNP was developed, demonstrating adequate sensitivity in a liquid solution with satisfactory specificity. The biosensor was optimized by adopting a transcriptional amplifier, which increased the maximum output by 149-fold, and improved the detection limit by 100-fold, from 1 mg/L to 10 μg/L. These biosensors had a linear range of 5–80 mg/L and 0.01–1.0 mg/L for PNP determination, respectively. Then, the agarose gel entrapment-based biosensor was constructed and allowed a good of PNP detection in the range of 5–60 mg/L in M9 solid agar within 70 min, and a detection sensitive of 16.8 mg/kg in soil. The good performance of the biosensor suggested its potential application of high-efficient and on-site detection in environmental matrices.
研究领域:
环境科学
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分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 6
·2211
·
IF 4.556
Wei Zhang,Danyang Li,Jianguang Zhang,Lingli Jiang,Zhaofa Li,Jun Sheng Lin
The compound 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane (o,p’-DDT) has been identified as one of the endocrine-disrupting chemicals causing adverse effects on wildlife and even humans through bioaccumulation. Its detection has become increasingly important. We have obtained candidate aptamers binding too,p’-DDT by a systematic evolution of ligands by exponential enrichment (SELEX) protocol. Five out of seventeen candidate sequences were selected for preliminary characterization by SYBR Green I assay. One sequence with highest fluorescence response witho,p’-DDT, designated DDT_13, was chosen for further characterization. Its dissociation constant (Kd) was determined to be 412.3 ± 124.6 nM. DDT_13 exhibited low cross-binding activities on other tested small molecules. The good bioactivities of DDT_13 were demonstrated for the analysis of spiked lake water and tap water samples. This study provides a novelo,p’-DDT-specific probe for its future applications.Keywords:o,p’-DDT;aptamer;SELEX;AuNPs;biosensor;real water sample
研究领域:
环境科学
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Cancer Biomarkers
·
2019
·Vol. 24
·期 4
·469-475
·
IF 2.859
Qin Yujing,Li Wenxue,Long Yingli,Zhan Zhijia
OBJECTIVE: This study aims to determine the correlation between p-cofilin expression and cisplatin resistance in patients with ovarian cancer, and also to investigate the role of p-cofilin in prognosis. PATIENTS AND METHODS: The ovarian cancer cell line A2780/DDP resistant to cisplatin was prepared. The cell resistance to cisplatin was measured via MTT assay. The cell invasion capacity was identified via Transwell assay. The mRNA expression and protein level was evaluated via semi-quantitative PCR and Western blot, respectively. The tumor tissues of patients with cisplatin-resistant ovarian cancer were collected. The relationship between prognosis and p-cofilin expression was analyzed. RESULTS: The growth rate of A2780 was similar to that of A2780/DDP. The sensitivity of A2780 to cisplatin was significantly higher than that of A2780/DDP (p< 0.01). The migration capacity of A2780/DDP was significantly increased compared to that of A2780 (p< 0.01), indicating that the cisplatin-resistant cell lines were successfully constructed. Both CFL1 mRNA level and p-cofilin level in A2780/DDP was significantly higher than that in A2780 (p< 0.01). The p-cofilin level in cancer tissues in patients with cisplatin-resistant ovarian cancer was significantly higher than that in patients with cisplatin-sensitive ovarian cancer (p< 0.01). The cisplatin resistance was positively correlated with the p-cofilin expression level (r= 0.802, p= 0.023). The survival time of patients with normal or low level of p-cofilin was significantly longer than that of patients with high expression. CONCLUSION: The cisplatin resistance of ovarian cancer is closely related to the expression level of p-cofilin, which affects the prognosis of patients with ovarian cancer.
研究领域:
肿瘤学
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分子生物学
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癌症研究
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DOI
PubMed
高影响因子
Acta Metallurgica Sinica-English Letters
·
2022
·Vol. 35
·期 10
·1653-1664
·
IF 3.024
Xie Shenghui,Liao Xiang,Zeng Xierong,Yang Haipeng,He Liang
High-entropy alloys (HEAs) have attracted widespread attention due to their excellent mechanical properties. However, their functional properties arising from the supersaturated solid solution state can be improved. Advanced oxidation processes (AOPs) are considered to be an effective method for decomposing organic pollutants, and HEAs are proposed as effective candidates for AOP catalysts. Currently, several HEAs used for organic pollutant degradation are prepared through mechanical alloying. In this work, FeAlCoNi and FeAlCoNiB Fenton-like catalysts were prepared by single roll melt spinning and subsequent ball milling techniques. We found that the FeAlCoNiB HEA exhibits highly efficient degradation for p -nitrophenol ( p -NP). The degradation efficiency of FeAlCoNiB is 120 times higher than that of FeAlCoNi, which confirms that boron functions effectively during the degradation process. FeAlCoNiB has a better degradation efficiency than pure iron powder and a degradation efficiency comparable to that of the amorphous Fe 78 Si 11 B 11 alloy. Additionally, the degradation efficiency of FeAlCoNiB X increases ( k values from 0.13 to 0.55 min −1 ) when the boron content increases from an X value of 0.25 to 1.25. We speculate that Fe 2 B favors the degradation process and promotes zero-valent iron to decompose organic pollutants through a galvanic cell effect. Possible p -NP degradation pathways are proposed after LC–MS analysis. This study provides a new series of HEA Fenton-like catalysts, which have the potential to be prepared into bulk devices for industrial applications.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2025
·
IF 2.3
Caidi Zhang,Wanchuan Shang,Xiaoning Zhang,Xueli Huang,Xueying Wang
Cresol is an important phenolic compound derived primarily from low-temperature coal tar. The presence of m/p-cresol increases the unsaturated bonds in coal tar. This increases the hydrogen consumption in the hydrogenation process. Separation and further purification of m/p-cresol can improve the value of coal tar. The boiling points of m-cresol and p-cresol are similar and therefore difficult to separate. It has become a major problem in the development of crude phenol refining. In this work, a novel complexing agent, hexamethylenetetramine, was used to complex and separate cresols. The effects of crystallization temperature, complexing agent dosage, solvent dosage and crystallization time were investigated. The process was optimized using response the surface methodology. As a result, the purity of m-cresol could reach more than 94.2% and the yield reached more than 30.4%, indicating that the process could effectively separate the mixture of m-cresol and p-cresol.
研究领域:
化学工程
·
煤化工
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产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 52
·46270-46277
·
IF 3.289
Wei Shao,Jimin Wu,Hui Liu,Guanghui Dong,Shuxia Wang,Huihua Min,Min Huang
Bacterial adhesion on the surfaces of medical devices, food processing equipment, heat exchangers and ship hulls has been recognized as a widespread problem. Bacterial adhesion on a surface is influenced by surface physical and chemical properties of the surface. In this paper, polyethyleneimine (PEI) grafted graphene oxide (GO) nanosheets were incorporated into Ni–P coatings by an electroless plating technique to modify the surface energy, and therefore to influence the interactions between the prepared surfaces and the tested bacteria. Ni–P–GO coatings were investigated by SEM, AFM, Raman spectrum and XPS. Contact angles of Ni–P–GO coatings with different GO loadings were tested and the surface free energies and their dispersive and polar components were calculated using the van Oss acid–base approach. The results showed that the surface free energies of the coatings had a significant influence on S. aureus adhesion. The Ni–P–GO coatings showed excellent antibacterial adhesion activity. Extended DLVO theory is used to explain the antibacterial adhesion behavior. The novel coatings reported here can be used in controlling bacterial adhesion and biofilm formation for various applications.
研究领域:
材料科学
·
微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2025
·
IF 7.9
Huiying Cheng,Jingchang Sun,Siwen Liu,Furong Cao,Liuting Shan,Zijiang Yang,Xiaofan Cui,Jiaxu Zhao,Zhen Qiao,Qiuju Feng
Low-grade heat (<100 °C) is abundant and ubiquitous in the environment, and the recovery of low-grade heat energy will be of great significance for the energy supply and the improvement of the environment. Low-cost and effective technologies for transforming low-grade heat into electricity are urgently needed. Thermocell (TEC) based on REDOX couples has become a promising technology for low-grade heat harvesting due to its advantages of low cost, scalability, and intrinsic high thermopower. To enhance its performance, different types of TECs need to incorporate various additives into the REDOX couples. However, there is currently no identical additive that effectively enhances the performance of both P-type and N-type TECs. In this paper, carboxylated chitosan is used as an identical additive, resulting in an increase in the Seebeck coefficient of P-type and N-type TECs by 115.33 % and 53.25 %, respectively. Furthermore, we introduce a novel sandwich-structured packing technique featuring a configuration of Metal/PET film/Carbon felt (MPC) for TEC. This approach effectively reduces both the cost and weight of the TEC, while the MPC structure further enhances their performance. The TEC can be scaled up to power electrical devices. Our achievement provides an effective strategy for harvesting low-grade heat energy.
研究领域:
能源材料
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CURRENT DRUG METABOLISM
·
2024
·Vol. 25
·期 5
·317-329
·
IF 2.1
Xiaocui Li,Qi Liang,Caiyan Wang,Huawei Qiu,Tingting Lin,Wentao Li,Rong Zhang,Zhongqiu Liu,Lijun Zhu
BackgroundYunaconitine (YAC) is a hidden toxin that greatly threatens the life safety of patients who are prescribed herbal medicines containing Aconitum species; however, its underlying mechanism remains unclear.ObjectiveThe objective of this study is to elucidate the functions of P-glycoprotein (P-gp) in regulating the efficacy, toxicity, and pharmacokinetics of YAC.MethodsThe efflux function of P-gp on YAC was explored by using Caco-2 monolayers in combination with the P-gp inhibitor verapamil. The impact of P-gp on regulating the analgesic and anti-inflammatory effects, acute toxicity, tissue distribution, and pharmacokinetics of YAC was determined via male Mdr1a gene knocked-out mice and wild-type FVB mice.ResultsThe presence of verapamil significantly decreased the efflux ratio of YAC from 20.41 to 1.07 in Caco-2 monolayers (P < 0.05). Moreover, oral administration of 0.07 and 0.14 mg/kg YAC resulted in a notable decrease in writhing times in Mdr1a-/- mice by 23.53% and 49.27%, respectively, compared to wild-type FVB mice (P < 0.05). Additionally, the deficiency of P-gp remarkably decreased the half-lethal dose (LD50) of YAC from 2.13 to 0.24 mg/kg (P < 0.05). Moreover, the concentrations of YAC in the tissues of Mdr1a-/- mice were statistically higher than those in wild-type FVB mice (P < 0.05). Particularly, the brain accumulation of YAC in Mdr1a-/- mice significantly increased by 12- and 19-fold, respectively, after oral administration for 30 and 120 min, when compared to wild-type FVB mice (P < 0.05). There were no significant differences in the pharmacokinetic characteristics of YAC between Mdr1a-/- and wild-type FVB mice.ConclusionYAC is a sensitive substrate of P-gp. The absence of P-gp enhances the analgesic effect and toxicity of YAC by upregulating its brain accumulation. Co-administration with a P-gp inhibitor may lead to severe YAC poisoning.
研究领域:
药理学
·
毒理学
·
中药学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2020
·
IF 3.693
Li Yujiao,Li Chen,Yu Huanqin,Yuan Beilei,Xu Fan,Wei Haoming,Cao Bingqiang
To regulate the optical and electrical properties of the crystals and films of the intrinsic methylammonium lead iodide (CH3NH3PbI3), we dope them with sodium (Na) by selecting sodium iodide (NaI) as a dopant source. The highly conductive p-type sodium-doped CH3NH3PbI3 (MAPbI3: Na) perovskite single crystals and thin films are successfully grown using the inverse temperature crystallization (ITC) method and antisolvent spin-coating (ASC) method, respectively. With the increase of Na+ doping concentration, the grain size of the film increases, the surface becomes smoother, and the crystallinity improves. Hall effect results demonstrate that both the MAPbI3: Na thin films and single crystals change their quasi-insulating intrinsic conductivity to a highly conductive p-type conductivity. The room-temperature photoluminescence (PL) peaks of doped MAPbI3 films slightly blue shift, while the photocarriers' lifetime becomes longer. The optical fingerprints of the doped levels in MAPbI3: Na perovskites can be identified by temperature-dependent PL. Obvious fingerprints of Na-related acceptor (A0X) levels in the doped MAPbI3: Na were observed at 10 K. These results suggest that sodium doping is an effective way to grow highly conductive p-type MAPbI3 perovskites.
研究领域:
材料科学
·
物理化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Zhijie Cui,Zhibo Yan,Jie Yin,Wenpin Wang,Mei-E Yue,Zhongcheng Li
Tailoring surface composition and coordinative environment of catalysts in a nano-meter region often influence their chemical performance. It is reported that CoP exhibits a low dissociation ability of H–OH, originating from the poor desorption of intermediate species. Herein, we provide a feasible method to construct P-Fe 2 O 3 -CoP nanosheets through a gas-phase phosphorization process. P doping induces the formation of interfacial structure between Fe 2 O 3 and CoP and the generation of defective structures. The resulting P-Fe 2 O 3 -CoP nanosheets afford high freshwater/seawater oxidation activity (250/270 mV@10 mA/cm 2 ) in 1 mol/L (M) KOH, which is even lower than commercial RuO 2 . Compared with CoP||CoP, P-Fe 2 O 3 ||P-Fe 2 O 3 , and Co 3 O 4 ||Co 3 O 4 , the assembled P-Fe 2 O 3 -CoP||P-Fe 2 O 3 -CoP exhibits the superior water/seawater electrolysis performance with 1.61/1.65 V@10 mA/cm 2 . The synergistic effect of P doping, defective structure, and heterojunction leads to high water oxidation efficiency and water splitting efficiency.
研究领域:
材料科学
·
催化化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）