INTERNATIONAL IMMUNOPHARMACOLOGY
·
2015
·
IF 2.472
Qiong Shi,Xiufang Song,Juanli Fu,Chuanyang Su,Xiaomin Xia,Erqun Song,Yang Song
The present study evaluated the protective effect of artificial sweetener neohesperidin dihydrochalcone (NHDC) against paraquat (PQ)-induced acute liver injury in mice. A single dose of PQ (75 mg/kg body weight, i.p.) induced acute liver toxicity with the evidences of increased liver damage biomarkers, aspartate transaminase (AST) and alanine transaminase (ALT) activities in serum. Consistently, PQ decreased the antioxidant capacity by reducing glutathione peroxidase (GP-X), glutathione-S-transferase (GST) and catalase (CAT) activities, glutathione (GSH) level and total antioxidant capacity (T-AOC), as well as increasing reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) levels. Histopathological examination revealed that PQ induced numerous changes in the liver tissues. Immunochemical staining assay indicated the upregulation of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions. However, NHDC ameliorates PQ-induced hepatic toxicity in mice by reversing these parameters. Additionally, NHDC significantly inhibited PQ-induced nuclear factor-kappa B (NF-κB) expression and mitochondrial-driven apoptotic signaling. TUNEL assay confirmed that PQ-induced apoptosis was relieved by NHDC. In conclusion, these findings suggested that NHDC showed potent antioxidant, anti-inflammatory and anti-apoptotic effects against PQ-induced acute liver damage.
研究领域:
药理学
·
毒理学
·
肝病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Jiangtao Dong,Aohui Han,Yanli Zhao,Haoming Li,Yue Yang,Bowen Yuan,Yishan Wang,Runqiang Liu,Xinming Yin,Xuezhong Du
Nanopesticides have been selected as one of the top 10 chemical innovations for enhancing the efficacy and safety of agrochemicals . Herein, smart, degradable, and eco-friendly metal−organic framework MIL-101(Fe III ) nanoherbicides coated with carboxymethyl cellulose-Ca II (CMC-Ca II ) cross-linking hydrogel-like networks are synthesized via a simple strategy. The coating of the CMC-Ca II hydrogel-like gatekeepers is oriented by the coordination unsaturated Fe III clusters on the surfaces of the MIL-101(Fe III ) nanocarriers to form a dense film network to prevent paraquat (PQ) leakage. Based on the stimuli factors (acid/basic pH, GSH, phosphates, and EDTA) of physiological and natural environments of target plants, the nanoherbicides are combined with five stimuli-responsive properties to attain the various controlled release of packaged PQ by the disassembly of the gatekeepers and/or the degradation of the MOF skeleton structure. More importantly, based on the stimuli-responsive controlled release mechanisms, the eco-friendly nanocarriers are ultimately degraded against bioaccumulation in plants or soil. The coating of natural CMC could promote the spreading of PQ owing to improvement of wettability for aqueous droplets of nanoherbicides on hydrophobic foliage. The PQ trapped in nanocarriers can effectively prevent PQ degradation, which showed that cumulative degradation rate is ca. 2.6 times lower than that of technical PQ under UV irradiation. The prepared nanoherbicides loaded with PQ show good control efficacy against weeds by controlling the release of PQ; good safety on seed germination (germination rate 97.32–99.67 %), seedling emergence (emergence rate 95.53–99.67 %), and are beneficial for the growth of wheat seedling (increase rate of plant height 1.89–6.97 % and 0.54–5.67 % after 7 and 15 days of seedling emergence, respectively) in the greenhouse; good biosafety for honeybees ( Apis mellifera L. ), which shows that lethal rates were 2.04 and 2.55 times lower than technical PQ for incubation 24 and 48 h, respectively. The nanoherbicides have potential applications in the field for PQ green agriculture.
研究领域:
农业科学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Zheng Zhao,Xue Yang
Paraquat (PQ) poisoning leads to irreversible fibrosis in the lungs with high mortality and no known antidote. In this study, we investigated the effect of the SET and MYND domain containing 2 (SMYD2) on PQ-induced pulmonary fibrosis (PF) and its potential mechanisms. We established an in vivo PQ-induced PF mouse model by intraperitoneal injection of PQ (20 mg/kg) and in vitro PQ (25 μM)-injured MLE-12 cell model. On the 15th day of administration, tissue injury, inflammation, and fibrosis in mice were evaluated using various methods including routine blood counts, blood biochemistry, blood gas analysis, western blotting, H&E staining, ELISA, Masson staining, and immunofluorescence. The findings indicated that AZ505 administration mitigated tissue damage, inflammation, and collagen deposition in PQ-poisoned mice. Mechanistically, both in vivo and in vitro experiments revealed that AZ505 treatment suppressed the PQ-induced epithelial-mesenchymal transition (EMT) process by downregulating GLI pathogenesis related 2 (GLIPR2) and ERK/p38 pathway. Further investigations demonstrated that SMYD2 inhibition decreased GLIPR2 methylation and facilitated GLIPR2 ubiquitination, leading to GLIPR2 destabilization in PQ-exposed MLE-12 cells. Moreover, rescue experiments conducted in vitro demonstrated that GLIPR2 overexpression eliminated the inhibitory effect of AZ505 on the ERK/p38 pathway and EMT. Our results reveal that the SMYD2 inhibitor AZ505 may act as a novel therapeutic candidate to suppress the EMT process by modulating the GLIPR2/ERK/p38 axis in PQ-induced PF.
研究领域:
药理学
·
毒理学
·
细胞生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Jing Sun,Fang Guo,Qiaofang Shi,Huayu Wu,Yan Sun,Ming Chen,Guowang Diao
In this paper, silver nanoparticles (AgNPs) modified by carboxylated pillar[5]arene (CP5) were anchored on graphene oxide (GO) by π-π stacking interaction and hydrogen bond between CP5 molecules and GO. The obtained hybrid nanomaterials CP5-AgNPs/GO were characterized by ultraviolet-visible adsorption spectrometer, Fourier transform infrared adsorption spectrometer, and transmission electron microscopy . Testing of various electrochemical techniques displays that the CP5-AgNPs/GO modified glass carbon electrodes (GCE) show good electrocatalytic property , molecular recognition and enrichment performance toward paraquat (PQ) and exhibit high current response. Compared with other modified electrodes , such as GO/GCE and CP5/GO/GCE, CP5-AgNPs/GO/GCE shows an outstanding electrochemical response for PQ with high sensitivity of 78.8 mA mmol −1 L cm −2 . The analytical curves for PQ obtained by differential pulse voltammetry are linear in the range from 1.0 × 10 −8 to 1.0 × 10 −5 M and from 2.0 × 10 −5 to 7.0 × 10 −5 M. The limit of detection is 1.0 × 10 −8 M (S/N = 3). This excellent performance is ascribed to the synergic electrocatalytic effect of AgNPs and GO, and the host-guest recognition of CP5 for PQ.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2014
·Vol. 67
·期 4
·593-600
·
IF 1.96
Zhang Weiguo,Liu Min,Zhang Peiliang,Yu Fugen,Lu Shan,Li Pengfu,Zhou Junying
Only limited information is available on herbicide toxicity to algae under mixotrophic conditions. In the present study, we studied the effects of the herbicide paraquat on growth, photosynthetic pigments, antioxidant enzymes, and gene expression in Chlorella pyrenoidosa under mixotrophic compared with autotrophic conditions. The mean measured exposure concentrations of paraquat under mixotrophic and autotrophic conditions were in the range of 0.3–3.4 and 0.6–3.6 μM, respectively. Exposure to paraquat for 72 h under both autotrophic and mixotrophic conditions induced decreased growth and chlorophyll (Chl) content, increased superoxide dismutase and peroxidase activities, and decreased transcript abundances of three photosynthesis-related genes (light-independent protochlorophyllide reductase subunit, photosystem II protein D1, and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [ rbc L]). Compared with autotrophic conditions, the inhibition percentage of growth rate under mixotrophic conditions was lower at 0.8 μM paraquat, whereas it was greater at 1.8 and 3.4 μM paraquat. With exposure to 0.8–3.4 μM paraquat, the inhibition rates of Chl a and b content under mixotrophic conditions (43.1–52.4 % and 54.6–59.7 %, respectively) were greater compared with autotrophic conditions, whereas the inhibition rate of rbc L gene transcription under mixotrophic conditions (35.7–44.0 %) was lower. These data showed that similar to autotrophic conditions, paraquat affected the activities of antioxidant enzymes and decreased Chl synthesis and transcription of photosynthesis-related genes in C. pyrenoidosa under mixotrophic conditions, but a differential susceptibility to paraquat toxicity occurred between autotrophically versus mixotrophically grown cells.
研究领域:
环境科学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yuanxia Lv,Xiaohuan Qin,Kun Hu,Fanggui Ye,Shulin Zhao
This study proposes a surface-assisted laser desorption ionization time-of-flight mass spectrometry (SALDI-TOF MS) method using an ultrastable microporous hydrogen-bonded organic frameworks (MP-HOFs) for selective and sensitive analysis of two common organic pesticides: paraquat (PQ) and chlormequat (CQ) in real samples. MP-HOFs are formed by H-bonding self-assembly of co-planar π-conjugated large aromatic tectons with carboxylate groups. They can not only efficiently absorb and transfer laser energy, but also specifically capture and isolate target analytes from complicated systems. The proposed SALDI-TOF MS method exhibited high salt tolerance and low background. The selective enrichment performance of MP-HOFs was demonstrated by analyzing PQ and CQ in real samples. Simultaneous analysis results of PQ and CQ showed satisfactory reproducibility with relative standard deviations (RSDs) of 3.2–9.2% and 5.7–12.3% for spot-to-spot and sample-to-sample, good linearity (R 2 > 0.99), and high sensitivity with a limit of detection of 0.001 and 0.05 ng mL −1 , respectively. The detection limits of PQ and CQ test analytes were three and two orders of magnitude lower than those of non-enriched PQ and CQ, respectively. Furthermore, the possible ionization mechanism was discussed, and the presented approach was successfully applied for PQ and CQ detection in tap water, river water, and soil, confirming its potential in practical applications.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Hongbin Pu,Yuting Xie,Qingyi Wei,Da-Wen Sun
SERS sensors based on metal-organic frameworks (MOFs) have garnered considerable attention in trace pesticide residue detection. UiO-66/AuNPs suspension substrates were fabricated by introducing thioglycolic acid (TGA) as a binder to modify the surface of AuNPs to facilitate their electrostatic assembly with UiO-66 prepared through hydrothermal reaction. The proposed substrates were used for quantitative and simultaneous analysis of paraquat and diquat in cabbage. Diquat and paraquat in actual cabbage samples were individually analysed with detection of limits (LOD) of 6 μg/L and 3 μg/L, respectively, and validated with recoveries ranging from 92.65 to 118.34%. Furthermore, the UiO-66/AuNPs substrate was able to differentiate between paraquat and diquat mixtures, even for the concentrations in the cabbage extracts differed by a factor of 100. The proposed UiO-66/AuNPs substrate provides a potentially useful tool for the trace determination of pesticide residues in the practical food safety analysis
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE CHEMICAL LETTERS
·
2022
·
IF 8.455
Meng Li,Xinzhong Wang,Yelin Zhu,Xiuxiu Jia,Shusheng Zhang,Huaisheng Wang,Yongtao Li,Guangzhi Hu
Boron/nitrogen-co-doped carbon (BCN) nanosheets decorated with Fe 2 O 3 nanocrystals (Fe 2 O 3 –BCN) were cast on a glassy carbon electrode (GCE) and applied as an electrochemical sensor to effectively detect paraquat (PQ), a toxic herbicide, in aqueous environments. A linear experiment performed using square wave voltammetry (SWV) under optimized experimental conditions produced a decent linear relationship and a low detection limit (LOD) of 2.74 nmol/L (S/N = 3). Repeatability, reproducibility, stability, and interference experiments confirmed that the Fe 2 O 3 –BCN/GCE system exhibited decent electrochemical sensing performance for PQ molecules. Notably, the designed sensor showed high selectivity and a decent linear relationship with PQ concentration in natural water samples. To the best of our knowledge, this is the first study on the preparation of Fe 2 O 3 –BCN nanosheets for PQ detection. The proposed sensor can be employed as an effective alternative tool for distinguishing and processing PQ.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yuanyuan Liu,Bangyan Liu,Lian Xia,Hongyan Yu,Qiaodie Wang,Yuangen Wu
Although methods based on mass spectrometry or immunoassays can meet the demands of paraquat detection, their application is limited by a lack of convenience or stability. Herein, a facile visual aptamer-based chromatographic strip (CS) was fabricated for the rapid paraquat detection using the cationic polyelectrolyte poly(diallyl dimethyl ammonium chloride) (PDDA) as a powerful capture molecule at test-line (T-line). The PDDA could capture the gold nanoparticles (AuNPs) through strong electrostatic interactions for visual detection. The proposed CS showed an excellent response for paraquat in the range of 20–150 μg·L −1 , and the limit of detection (LOD) was as low as 4.28 μg·L −1 . This detection method has good stability and specific selectivity , and can visually determine paraquat residues more than 30 μg·L −1 by naked eye within 3 min. The results demonstrated that the prepared CS based on aptamer and cationic polyelectrolyte is an accurate and practicable tool for rapid analysis of pesticide residues .
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 20
·3632
·
IF 5.719
Weiran Zheng,Ruobing Su,Guoguang Yu,Lin Liu,Fei Yan
Herein, we demonstrate a sensitive and rapid electrochemical method for the detection of paraquat (PQ) using a glassy carbon electrode (GCE) modified with vertically ordered mesoporous silica films (VMSF) and a nanocarbon composite. The three-dimensional graphene-carbon nanotube (3DG-CNT) nanocarbon composite has a 3D network structure, a large electroactive area and oxygen-containing groups, promoting electron transfer between PQ and the underlying electrode and providing a suitable microenvironment for the stable growth of VMSF. This VMSF/3DG-CNT nanocomposite film could be prepared on the GCE’s surface by a two-step electrochemical method with good controllability and convenience. Owing to the synergistic effect of the electrocatalytic ability of 3DG-CNT and the electrostatically enriched capacity of VMSF, the proposed VMSF/3DG-CNT/GCE has superior analytical sensitivity compared with the bare GCE. Furthermore, VMSF has excellent anti-fouling ability that makes the fabricated sensor exhibit satisfactory performance for direct analysis of PQ in environmental water samples.Keywords:paraquat;electrochemically reduced graphene oxide;carbon nanotubes;vertically ordered mesoporous silica films;electrochemical detection
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 4
·1769-1780
·
IF 3.9
Yumin Chen,Linxuan Zhu,Yuling Yang,Di Wu,Yan Zhang,Weiwei Cheng,Xiaozhi Tang
A high-performance Au@MIL-101/PMMA/DT surface-enhanced Raman scattering (SERS) substrate was fabricated for sensitive and stable detection of paraquat by self-assembling metal organic framework–modified Au nanoparticles (Au@MIL-101) on a poly(methyl methacrylate) (PMMA) film and then immobilizing the formed substrate onto a duct tape (DT). The highly closely packed Au@MIL-101 array provided intensive hotspots for SERS sensing. The MIL-101 layer modified on the surface of Au nanoparticles could absorb paraquat to the electromagnetic enhancement area of Au nanoparticles. The DT on the bottom made the substrate smoother, which is beneficial for achieving a more stable detection performance. As a result, the constructed substrate exhibited outstanding uniformity with relative standard deviations of 9.47% and storage stability for 2 months. For detecting paraquat, the substrate showed a low detection limit of 7.1 × 10 −9 M (1.83 µg/kg) and wide linear range from 10 −8 to 10 −2 M. Furthermore, the substrate showed good detection performance in real cereal samples with desirable recovery rates from 91.57% to 102.32%.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Kaidong Wang,Chunhui Zhang,Baofu Zhang,Guoliang Li,Ge Shi,Qian Cai,Min Huang
Background One of the most common types of neurodegenerative diseases (NDDs) is Lewy body disease (LBD), which is characterized by excessive accumulation of α-synuclein (α-syn) in the neurons and affects around 6 million individuals globally. In recent years, due to the environmental factors that can affect the development of this condition, such as exposure to herbicides and pesticides, so it has become a younger disease. Currently, the vast majority of studies on the neurotoxic effects of paraquat (PQ) focus on the late mechanisms of neuronal-glial network regulation, and little is known about the early origins of this environmental factor leading to LBD. Objective To observe the effect of PQ exposure on intestinal function and to explore the key components of communicating the gut-brain axis by establishing a mouse model. Methods and results In this study, C57BL/6J mice were treated by intraperitoneal injection of 15 mg/kg PQ to construct an LBD time-series model, and confirmed by neurobehavioral testing and pathological examination. After PQ exposure, on the one hand, we found that fecal particle counts and moisture content were abnormal. on the other hand, we found that the expression levels of colonic tight junction proteins decreased, the expression levels of inflammatory markers increased, and the diversity and abundance of gut microbiota altered. In addition, pathological aggregation of α-syn was consistent in the colon and midbrain, and the metabolism and utilization of short-chain fatty acids (SCFAs) were also markedly altered. This suggests that pathological α-syn and SCFAs form the gut may be key components of the communicating gut-brain axis. Conclusion In this PQ-induced mouse model, gut microbiota disruption, intestinal epithelial barrier damage, and inflammatory responses may be the main causes of gut dysfunction, and pathological α-syn and SCFAs in the gut may be key components of the communicating gut-brain axis.
研究领域:
神经科学
·
毒理学
·
微生物学
·
病理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）