FOOD CHEMISTRY
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2024
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IF 8.5
Fuyuan Zhang,Yamei Li,Seid Mahdi Jafari,Yang Liu,Yaxin Sang,Shuo Wang,Xianghong Wang
Gold nanoparticles (AuNPs)-based immunochromatographic assay has gained popularity as a rapid detection method for food hazards. Synthesizing highly stable AuNPs in a rapid, simple and environmentally friendly manner is a key focus in this field. Here, we present a green microfluidic strategy for the rapid, automated, and size-controllable synthesis of pepsin-doped AuNPs (AuNPs@Pep) by employing glucose-pepsin as a versatile reducing agent and stabilizer. Through combining the colorimetric and photothermal (PoT) properties of AuNPs@Pep, both “signal-off” and “signal-on” formats of microfluidic paper analytical devices (PADs) were developed for detection of a small molecule antibiotic, florfenicol, and an egg allergen, ovalbumin. Compared to the colorimetric mode, a 4-fold and 3-fold improvement in limit of detection was observed in the “signal-off” detection of florfenicol and the “signal-on” detection of ovalbumin, respectively. The results demonstrated the practicality of AuNPs@Pep as a colorimetric/PoT dual-readout probe for immunochromatographic detection of food hazards at different molecular scales.
研究领域:
分析化学
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纳米技术
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食品科学
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环境科学
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DOI
PubMed
高影响因子
Scientific Reports
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2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Jiang Bin,Zhang Xiaoqing,Yuan Yongqiang,Qu Yuxiao,Feng Zhibiao
An aqueous two-phase system (ATPS) consisting of poly(ethylene glycol-ran-propylene glycol) monobutyl ether (UCON)/phosphate was developed for the separation of the antioxidant peptides from pepsin hydrolysate of Whey Protein Isolate (WPI). The efficiency of the separation was evaluated based on the DPPH radical scavenging activity, ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) of the separated peptides. The effects of some parameters on the partition of antioxidant peptides were investigated. An efficient separation of antioxidant peptides was achieved using ATPS with pH of 4.0, 4 mL of UCON solution (40%, w / w ), 4 mL of KH 2 PO 4 solution (15.5%, w / w ), 2 mL of WPI hydrolysate and 0.40 g/10 mL of NaCl. Reversed-phase high-performance liquid chromatography (RP-HPLC), amino acid analyzer and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to characterize the purified peptides separated by the ATPS. The peptides in top phase were less polar than those in bottom phase. More antioxidative and hydrophobic amino acids were extracted to the top phase of ATPS, and the peptides with the amino acid sequences with antioxidant activities moved to the top phase as well. In conclusion, antioxidant peptides were successfully separated from the WPI hydrolysate by UCON/phosphate ATPS.
研究领域:
生物化学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Ge Ge,Jie Zhao,Jiabao Zheng,Mouming Zhao,Weizheng Sun
This study aimed to investigate the effect of tea polyphenol extract (TPE) on the in vitro gastric digestion of soymilk. Fluorescence recovery after photobleaching was applied to measure pepsin diffusivity in soymilk. The characteristics of soymilk digesta were evaluated by gel electrophoresis, degree of hydrolysis (DH), molecular weight distribution, free amino acid analysis, particle size, antioxidant capacity, and trypsin/chymotrypsin inhibitor activity (TIA/CIA). The binding between soy proteins and tea polyphenols could significantly impair in vitro gastric digestion of soymilk by decreasing pepsin diffusivity from 91.3 to 70.3 μm2/s and DH from 17.13 to 13.93% with 1.2 mg/g TPE addition. Soymilk with 0.6 mg/g TPE addition exhibited low TIA/CIA and a strong antioxidant capacity in gastric digesta, which might be good for the following intestinal digestion. A better understanding of the effect of polyphenol on the digestion of protein-based food may be beneficial to innovation in food manufacturing.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Mengjiao Zhu,Jing Kong,Qing Li,Xinxin Liu,Jing Zhang,Tao Liu,Liqun Ren,Jing Zheng,Zhenyu Chu,Wanqin Jin
Aspiration pneumonia (AP), caused by the microinhalation of gastric contents, is easy to miss because of its nonspecific symptoms and is associated with a high mortality rate (46.1%) over a short disease course. Current diagnostic methods for aspiration pneumonia, including medical imaging and bronchoscopy, are difficult to use for onsite and rapid diagnosis because they require bulky and time-consuming equipment. In this work, we propose an ultrasensitive electrochemical immunosensor to achieve fast determination of the AP biomarker (pepsin) in the bronchoalveolar lavage fluid (BALF) of AP patients. This immunosensor works through the construction of heterogeneous Ag@Co(OH)2 NWs to simultaneously provide both high electrocatalysis and high conductivity. In terms of architecture, Ag NWs can contribute abundant active sites for antibody immobilization to achieve target capture ability while accelerating electron transfer. In addition, the uniformly distributed Co(OH)2 nanoparticles intensify the response signal because of their high redox capacity. Through the above synergetic effects, our immunosensor enables the recognition and reporting of trace amounts of pepsin in patients’ BALF within only 20 min, which is at least two times faster than that of clinical techniques. Moreover, the high diagnostic accuracy of the immunosensor is comparable to that of CT images from clinical AP patients. This facile immunosensor is promising for providing new insights into the early diagnosis of AP to guide clinical treatment onsite.
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Xunyu Song,Yukun Song,Zixuan Guo,Mingqian Tan
Food-derived nanoparticles are inevitably ingested with food matrices and may negatively affect digestion. In this study, the bio-distribution of carbon nanoparticles (CNPs) extracted from roasted chicken in the gastrointestinal tract was investigated. Their biological effects on the digestion of soy protein isolate (SPI) with pepsin were explored by spectroscopic, morphological, and enzymatic methods. In vitro simulated digestion results showed that the digestibility of SPI was decreased from 93.53% to 79.56% in gastric juice with 10% CNPs. The CNPs were able to significantly reduce the activity of pepsin and disrupt its secondary structure ( P < 0.05). Ultraviolet–visible spectrometry demonstrated that CNPs exhibited a physical absorption effect on pepsin and quenched its fluorescence in a static model. The results of zeta potential and atomic force microscopy proved that the CNPs formed a nanoparticle-protein corona structure with pepsin, which could affect the digestion of SPI.
研究领域:
纳米技术
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食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2026
·
IF 3
Rong Hu,Yuxiang Wu,Chen Zhu,Liangying Liu,Ailin Zhang,Huahao Zhang,Yiquan Li,Shenhua Jiang
Wild tea is highly valued for its perceived superior quality, yet scientific validation remains limited. Therefore, this study comprehensively evaluated the quality of Wuning wild tea. Compared to cultivated tea, Wuning wild tea exhibits superior essential chemical constituents, including tea polyphenols and free amino acids, and its functional component composition and quality characteristics are better. Wuning wild black tea (WBT) was shown to possess the highest quality among the 11 teas evaluated through multivariate statistical analysis and the technique for ordering preferences for similarity to ideal solutions-gray relational analysis (TOPSIS-GRA) coupled model, and to possess strong antioxidant activity. Mechanistic studies revealed that the interaction between theaflavin-3,3′-digallate (TFDG) and pepsin (PEP) follows a static quenching mechanism. UV–vis spectroscopy analysis showed that TFDG reduces the hydrophobicity of tyrosine and tryptophan residues in PEP. These results provide a scientific basis for the superior quality of wild tea.
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2018
·
IF 2.88
Yabo Shi,Min Liu,Hui Yan,Chang Cai,Qingying Guo,Wenxin Pei,Ruiyan Zhang,Zhengping Wang,Jun Han
The individual and combined interactions of bisphenol F and piceatannol with pepsin were investigated using spectroscopic methods (fluorescence, UV–vis absorption, and circular dichroism spectroscopy), combined with isothermal titration calorimetry and molecular docking. Thermodynamic data showed that hydrogen bonds and van der Waals forces might play a major role for the binding process. Site marking experiments and molecular docking confirmed the binding sites of these two ligands on pepsin. The discrepancy in the binding constant between the binary and ternary systems indicated the competitive binding of piceatannol and bisphenol F to pepsin. Circular dichroism spectra studies suggested that the binding of the two ligands led to a loosening of pepsin backbone. Enzyme activity assays indicated that the inhibition of pepsin activity by piceatannol and bisphenol F was competitive. These results will be helpful to understand the mechanism of piceatannol and bisphenol F affecting the activity of digestive proteases in the sight of the food security.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Xiyao Tang,Jiafen Yu,Xinxin You,Yujia Liu,Hong Wang,Jihui Wang,Yanxue Cai
Curcumin, a natural phenolic compound with various bioactivities, is easily decomposed into stability ferulic acid and vanillin molecules. Whether the functional equivalence of decomposition products can match that of curcumin is a matter worth attention. In this work, the inhibition and interaction of curcumin and its decomposition products on pepsin were studied. The inhibition by curcumin and ferulic acid belonged to competitive inhibition, the quenching types were static quenching with spontaneous process. Binding energies of curcumin, ferulic acid and vanillin were −8.33 ± 0.14, −7.88 ± 0.09 and −6.12 ± 0.08 kcal/mol, respectively, through H-bonds and hydrophobic interactions. All molecules significantly changed the secondary structure of pepsin and reduced the deformation temperature about 15 °C. The results show that better inhibition on pepsin from curcumin than ferulic acid, whereas vanillin did not show inhibition. The results provided the experimental basis for curcumin as potential inhibitors of pepsin in special diets.
研究领域:
生物化学
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营养学
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产品关联
DOI
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Shengnan Hu,Ronghui Wu,Ming Guo
Metal-organic framework (MOF)-based immobilized enzymes with green catalytic and reusable features have been widely used in various fields. The pore structure modulation of MOF is the key to the application of MOF materials. The traditional MOF is only microporous, which affects the immobilization rate and catalytic activity of the enzyme. Here, ions that can interact with MOF coordination groups are used to induce the lattice structure of MOF to expand, forming porous carriers with high activity, which not only provides a stable microenvironment for pepsin, but also enables heterogeneous catalysis of pepsin. Pepsin was immobilized onto MOF carriers to form heterogeneous pepsin using immobilized enzyme technology, and the results showed that the immobilization rate of pepsin on MOF carriers modified with pore structure was increased from 49.25 % to 84.68 %. The prepared heterogeneous pepsin showed excellent removal performance of microplastic additive di-n-octyl phthalate (DNOP) contaminant with a removal rate of 95.68 % in 180 min, which is a completely innovative method for the removal of DNOP. In addition, the molecular models of heterogeneous pepsin and DNOP were established, the theoretical results were in agreement with the experimental results, and the degradation pathway of DNOP was analyzed by gas chromatography-mass spectrometry (GC–MS) technique. Our results demonstrate the potential of heterogeneous pepsin as a green catalyst, which can provide new ideas for the construction of heterogeneous enzyme catalysts and the removal of organic pollutants in the future.
研究领域:
催化
·
环境科学
·
材料科学
·
生物技术
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产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2020
·
IF 2.455
Ruichang Gao,Wanghui Shu,Yang Shen,Quancai Sun,Wengang Jin,Dajing Li,Ying Li,Li Yuan
Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6、TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPKs, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.
研究领域:
免疫学
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生物化学
·
药理学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Mingxuan Ma,Cheng Chen,Xinqi Zhu,Xiaoqi Li,Yingxiang Du,Liu Zhang,Jie Gan
New material zeolitic imidazolate framework-4, 5-imidazoledicarboxylic acid (ZIF-IMD) located on the pore surface of porous layer open-tubular (PLOT) column previously functionalized with N-(3-aminopropyl)-imidazole have been prepared via a layer-by-layer self-assembly strategy. This new ZIF-IMD coating hybrids are used as solid-phase carriers for chiral selector pepsin immobilization. The ZIF-IMD material was characterized by scanning electron microscopy, energy-dispersive spectroscopy, transmission electron microscope and X-ray diffraction. The synthesized pepsin@ZIF-IMD@POLT column achieved the baseline separation of hydroxychloroquine (HCQ), chloroquine (CHQ) and hydroxyzine (HXY) (the resolution of HCQ: 2.19; CHQ: 1.84; HXY: 1.53). Compared with the pepsin@PLOT column (without ZIF-IMD material), the chiral separation capability of the pepsin@ZIF-IMD@POLT column can be remarkably improved. Several key parameters including concentration of chiral selector, buffer pH, applied voltage and buffer concentration were systematically evaluated to provide the optimal enantioseparation condition. The relative standard deviations (RSDs) of intra-day, inter-day, column-to-column and inter-batch of migration time and Rs of the HCQ were evaluated in detail, respectively (RSD < 7.21%). Additionally, the potential mechanism of increased resolution was discussed in the article.
研究领域:
分析化学
·
材料科学
·
药物分析
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jie Zhao,Jihong Wu,Yanni Chen,Mouming Zhao,Weizheng Sun
The model of lipoxygenase-catalyzed linoleic acid (LA) oxidation was selected as representative of a lipid peroxidation system to investigate the effects of oxidative modification on soybean protein isolate (SPI) gel properties and in vitro gastric digestion. Fluorescence recovery after the photobleaching (FRAP) technique was applied to evaluate pepsin diffusion in the gel. The results showed that oxidative modification increased the gel hardness as well as brought about a compact and three-dimensional network structure, which consequently decreased the water mobility as manifest by lowering the relaxation time of T2b and T21 from 0.55 and 3.22 ms for the control to 0.32 and 2.42 ms for 7LA+LOX (addition of 7 mL of LA and LOX), respectively. It was interesting to note that pepsin diffusion was significant correlated (p < 0.05) with T2b and DH (degree of hydrolysis), indicating that water mobility might be a factor related to FITC–pepsin diffusion, which would ultimately influence the gel gastric digestion. Compared with native SPI, moderate oxidation can improve the digestibility of SPI gel by the summed effects of pepsin diffusion limitation, microstructure variation, and hydrolysis degree.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）