Nutrients
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2022
·Vol. 14
·期 5
·1013
·
IF 6.706
Mingfei Yao,Yiqiu Fei,Shuobo Zhang,Bo Qiu,Lian Zhu,Fang Li,Björn Berglund,Hang Xiao,Lanjuan Li
Resveratrol (RSV) has been confirmed to confer multiple health benefits, and the majority of RSV tends to be metabolized in the gut microbiota after oral administration. In this study, the metabolism of RSV was investigated by using mouse models with distinct gut microbiota compositions: germ-free mice colonized with probiotics, conventional mouse, and DSS-induced colitis mouse models. The results demonstrated that in feces, the metabolites of RSV, including resveratrol sulfate (RES-sulfate), resveratrol glucuronide (RES-glucuronide), and dihydroresveratrol, significantly increased after probiotics colonized in germ-free mice. Furthermore, RES-sulfate and RES-glucuronide were below the detectable limit in the feces of conventional mice, with dihydroresveratrol being the dominant metabolite. Compared to the conventional mice, the ratio ofFirmicutes/Bacteroidesand the abundance ofLactobacillusgenera were found significantly elevated in colitis mice after long-term ingestion of RSV, which shifted the metabolism of RSV in return. Our study provided critical implications in further application of RSV in foods and food supplements.Keywords:resveratrol (RSV);metabolites;gut microbiota;mouse model;Ligilactobacillus salivariousLi01
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Experimental and Therapeutic Medicine
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2022
·Vol. 24
·期 3
·1-10
·
IF 2.751
Zhi-Bin Dong,Yu-Jia Wang,Wen-Jun Wan,Ji Wu,Bo-Jun Wang,Hai-Li Zhu,Min Xie,Ling Liu
Oxaliplatin (OXA) is a common chemotherapy drug and exhibits clinical activity in several cancer types. Its anticancer clinical effect is frequently accompanied by neurotoxicity. The symptoms include paresthesia and pain, which adversely affect the quality of life of patients. In the present study, five consecutive intraperitoneal injections of 4 mg/kg OXA were used to mimic chemotherapy in rats. OXA administration induced mechanical allodynia, activated spinal astrocytes and triggered the inflammatory response. To explore potential therapeutic options for OXA‑induced neuropathic pain, resveratrol (Res) was intrathecally injected into the spinal cord of OXA‑treated rats. Paw withdrawal threshold values of OXA‑treated rats were increased, indicating an antinociception effect of Res on OXA‑induced pain. Additionally, Res treatment reduced the levels of glial fibrillary acidic protein, TNF‑α, IL‑1β and NF‑κB, which were upregulated in OXA‑treated rats (compared with control). Furthermore, Auto Dock data showed that Res binds to cyclooxygenase‑2 (COX‑2) through six hydrogen bonds. Western blot analysis and reactive oxygen species (ROS) assays indicated that Res treatment decreased COX‑2 expression and suppressed ROS production. In summary, intrathecal injection of Res reduced the spinal COX‑2‑mediated ROS generation and inflammatory reaction, suppressed astrocytic activation, and alleviated OXA‑induced neuropathic pain.
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JOURNAL OF MEMBRANE SCIENCE
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2021
·
IF 8.742
Youxiu Huo,Qingbing Li,Zhiyan Rui,Rui Ding,Jia Liu,Jia Li,Jianguo Liu
The need to reduce the cost of proton exchange membranes (PEMs) leads to less usage of perfluorinated sulfonic acid (PFSA), which would definitely result in a decline in the membrane lifespan under the attack of free radicals. In this study, polytetrafluoroethylene (PTFE) is used as a reinforced material to make a thinner composite membrane due to the poor mechanical strength of the pristine membrane. Moreover, a polydopamine (PD)-treated strategy was selected to make hydrophilic PTFE, which allows the adoption of a high-boiling point solvent for the dissolution of resveratrol , a natural antioxidant produced by plants that acts as a free radical scavenger (FRS) in the membrane to prevent the membrane from being attacked. With the help of dimethyl formamide (DMF), a high-boiling point solvent, and hydrophilic groups in PD molecules, a Nafion/PD@PTFE/resveratrol composite membrane with a thickness of 10 μm was prepared and tested using an accelerated degradation test (ADT) strategy. With a resveratrol dose of 1 wt%, the cell presented a lower hydrogen crossover of 2.82 mA cm −2 and a longer lifespan than those of a membrane without resveratrol while cell performance did not suffer too much damage. In the ADT experiment, the hydrogen crossover of Nafion/PD@PTFE/resveratrol was still less than 20 mA cm −2 after 8 open circuit voltage (OCV) cycles, while that without resveratrol increased to 80 mA cm −2 in 3 OCV cycles.
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iScience
·
2026
·Vol. 29
·期 10
·117733
·
IF 4.5
Yanlin Wu,Wenyu Yang,Yilan Zeng,Dingfeng Huang,Wenbo Dong,Olivier Monfort,Martin Motola
Natural resveratrol can assist Fe(III)-mediated PDS activation • RES promotes Fe(III) reduction and supports Fe(III)/Fe(II) cycling • Balanced RES concentration improves redox cycling while limiting radical competition • Near-neutral OFL degradation is achieved without additional pH adjustment Summary Iron-mediated peroxydisulfate (PDS) activation is limited by slow Fe(III)/Fe(II) redox cycling under circumneutral conditions. Here, resveratrol (RES), a natural stilbene polyphenol, was introduced as a low-dose redox mediator to promote Fe(II) formation from Fe(III) for enhanced PDS activation. The Fe(III)/RES/PDS system showed higher ofloxacin (OFL) degradation than the corresponding single-reagent and binary systems and achieved approximately 90% OFL degradation at pH 6.5. Chemical quenching and competition-kinetic analysis indicated that sulfate radical (SO 4 ·− ) was the major contributor to OFL degradation, while hydroxyl radical ( · OH) played a secondary role. Matrix experiments showed that Cl − and NO 3 − had minor effects, whereas NO 2 − strongly inhibited OFL degradation. This work provides a case study of RES-mediated Fe(III)/PDS activation for antibiotic degradation under near-neutral conditions. Download: Download high-res image (83KB) Download: Download full-size image
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Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
·
2018
·Vol. 301
·期 6
·1115-1125
·
IF 1.373
Hui Wang,Xiaoguang Dong,Zengxun Liu,Shaowei Zhu,Haili Liu,Wenchuang Fan,Yanlai Hu,Tao Hu,Yonghui Yu,Yizhao Li,Tianwei Liu,Chengjia Xie,Qing Gao,Guibao Li,Jing Zhang,Zhaoxi Ding,Jinhao Sun
Rotenone is a common pesticide and has been reported as one of the risk factors for Parkinson disease. Rotenone can cause neuronal death or apoptosis through inducing oxidative injury and inhibiting mitochondrial function. As a natural polyphenolic compound, resveratrol possesses the antioxidant capacity and neuroprotective effect. However, the mechanism underlying the neuroprotective effect of resveratrol against rotenone-induced neurotoxicity remains elusive. Here, we treated PC12 cells with rotenone to induce neurotoxicity, and the neurotoxic cells were subjected to resveratrol treatment. The CCK8 and LDH activity assays demonstrated that resveratrol could suppress neurotoxicity induced by rotenone ( P < 0.01). The DCFH-DA assay indicated that resveratrol reduced the production of reactive oxygen species (ROS). JC-1 and Hoechst 33342/PI staining revealed that resveratrol attenuated mitochondrial dysfunction and cell apoptosis. Moreover, resveratrol reversed rotenone-induced decrease in SIRT1 expression and Akt1 phosphorylation ( P < 0.05). Furthermore, when the SIRT1 and Akt1 activity was inhibited by niacinamide and LY294002, respectively, the neuroprotective effect of resveratrol was remarkably attenuated, which implied that SIRT1 and Akt1 could mediate this process and may be potential molecular targets for intervening rotenone-induced neurotoxicity. In summary, our study demonstrated that resveratrol reduced rotenone-induced oxidative damage, which was partly mediated through activation of the SIRT1/Akt1 signaling pathway. Our study launched a promising avenue for the potential application of resveratrol as a neuroprotective therapeutic agent in Parkinson disease. Anat Rec, 301:1115–1125, 2018. © 2018 Wiley Periodicals, Inc.
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PROCESS BIOCHEMISTRY
·
2016
·
IF 2.529
Ying-bo Li,Fen Liu,Hai-yan Luo,Yi-fan Zhu,Xiang-feng Liang,Hui-zhou Liu,Jian-jiang Zhong
In this work, the hydrolytic kinetic of piceid was systematically investigated not only in different solvents, but under different conditions of pH and temperature as well. Results showed that the solvents have a significant effect on rate constant. In general, piceid was more readily hydrolyzed in pure organic solvents in which the rate constant was over 10 times of that in conventional aqueous methanol. The solvent effect was analyzed using LSER and it demonstrated that hydrolysis of piceid is accelerated by solvents with larger value of dipolarity/polarizability, being inhibited by hydrogen bond donor solvents. Furthermore, the rate constant increased with elevated acidity and temperature, resulting in shorter time for hydrolysis of piceid and avoiding degradation of resveratrol. The hydrolysis of piceid obeyed first-order kinetics. The new information obtained herein could be helpful to enhanced production of resveratrol via hydrolysis of piceid.
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RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
·
2024
·Vol. 50
·期 4
·1466-1475
·
IF 1.1
Lu Liushen,Qin Ting,Chen Kai,Xie Jun,Pan Liangkun,Xi Bingwen
Abstract Objective: Resveratrol, a natural product found in plants, is a polyphenolic compound with strong antioxidant activity. In order to enhance the antioxidant activity of resveratrol, we have used a natural product emodin, which has multiple hydroxyl group structure similar to resveratrol. Methods: In this study, we investigated the antioxidant activities of resveratrol and emodin in vitro respectively, including their abilities to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, hydroxyl radicals, and superoxide radicals. Results and Discussion: our results demonstrated that the combination of resveratrol and emodin significantly enhances antioxidant activity. And the process of their DPPH radical scavenging follows a first-order reaction kinetics equation. Finally, a possible mechanism for the scavenging of DPPH free radicals by resveratrol and emodin was proposed. Conclusions: We found that emodin combined with resveratrol can increase antioxidant activity. This study provides a new sight to enhance antioxidant activity.
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PHYTOMEDICINE
·
2024
·
IF 6.7
Ziwei Huang,Shan Li,Lishan Zhong,Yuan Su,Menghe Li,Xiaohui Wang,Zexu Wang,Zhiping Wang,Cuifang Ye,Zhe Ren,Xiao Wang,Qiongzhen Zeng,Kai Zheng,Yifei Wang
Background Herpes simplex virus type 1 (HSV-1)-induced herpes simplex encephalitis (HSE) has a high mortality rate in clinically immunocompromised patients, while recovered patients often experience neurological sequelae due to neuroinflammation. Nucleoside drugs and nucleoside analogues such as acyclovir and ganciclovir are mainly used in clinical treatment, and the emergence of resistant viral strains makes the development of new anti-herpesvirus encephalitis drugs urgent. Resveratrol is a multifunctional, plant-derived bioactive compound and its antiviral potential is attracting much attention. Purpose This study aimed to investigate the anti-HSV-1 mechanism of resveratrol in microglial cells and in the HSE mouse model. Methods The antiviral effect of resveratrol on HSV-1 infection was investigated by plaque assay, virus titer, immunofluorescence, Western blot and time-of-addition assay. The influence of resveratrol on stimulator of interferon gene (STING)/Nuclear Factor kappa B (NF-κB) signaling pathway-mediated neuroinflammation was examined by Western blot, RT-qPCR and ELISA. The interaction between resveratrol and STING/heat shock protein 90 beta (HSP90β) was evaluated by molecular modeling, co-immunoprecipitation, and drug affinity responsive target stability assay. The therapeutic effect of resveratrol on HSE was evaluated in the HSE mouse model by analyzing weight loss, neurodegenerative symptoms and histopathological scores. Results Resveratrol inhibited the early process of HSV-1 infection, and interfered with the STING/NF-κB signaling pathway to attenuate HSV-1-induced neuroinflammation and microglial M1 polarization, independent of its classical target Sirtuin1. Mechanistically, resveratrol completely bound to Glu515 and Lys491 of HSP90β, thus disrupting the HSP90β-STING interaction and promoting STING degradation. Resveratrol also significantly alleviated viral encephalitis and neuroinflammation caused by HSV-1 in the HSE mouse model. Conclusion Resveratrol acted as a non-classical HSP90β inhibitor, binding to the STING-HSP90β interaction site to promote STING degradation and attenuate HSV-1-induced encephalitis and neuroinflammation. These findings suggest the alternative strategy of targeting HSP90β and resveratrol-mediated inhibition of HSP90β as a potential antiviral approach.
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Biomolecules
·
2024
·Vol. 14
·期 6
·712
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IF 4.8
Gehad G. Ibrahim,Madhavi Perera,Saadiah A. Abdulmalek,Jinyong Yan,Yunjun Yan
Resveratrol, a phenylpropanoid compound, exhibits diverse pharmacological properties, making it a valuable candidate for health and disease management. However, the demand for resveratrol exceeds the capacity of plant extraction methods, necessitating alternative production strategies. Microbial synthesis offers several advantages over plant-based approaches and presents a promising alternative.Yarrowia lipolyticastands out among microbial hosts due to its safe nature, abundant acetyl-CoA and malonyl-CoA availability, and robust pentose phosphate pathway. This study aimed to engineerY. lipolyticafor resveratrol production. The resveratrol biosynthetic pathway was integrated intoY. lipolyticaby adding genes encoding tyrosine ammonia lyase fromRhodotorula glutinis, 4-coumarate CoA ligase fromNicotiana tabacum, and stilbene synthase fromVitis vinifera. This resulted in the production of 14.3 mg/L resveratrol. A combination of endogenous and exogenous malonyl-CoA biosynthetic modules was introduced to enhance malonyl-CoA availability. This included genes encoding acetyl-CoA carboxylase 2 fromArabidopsis thaliana, malonyl-CoA synthase, and a malonate transporter protein fromBradyrhizobium diazoefficiens. These strategies increased resveratrol production to 51.8 mg/L. The further optimization of fermentation conditions and the utilization of sucrose as an effective carbon source in YP media enhanced the resveratrol concentration to 141 mg/L in flask fermentation. By combining these strategies, we achieved a titer of 400 mg/L resveratrol in a controlled fed-batch bioreactor. These findings demonstrate the efficacy ofY. lipolyticaas a platform for the de novo production of resveratrol and highlight the importance of metabolic engineering, enhancing malonyl-CoA availability, and media optimization for improved resveratrol production.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lin Zhang,Luzhen Chen,Shiyi Li,Zhenyu Yu,Yibin Zhou,Yongquan Wang
This study aimed to improve the mechanical properties of wheat starch gels (WSG) and the stability and bioaccessibility of resveratrol (Res) in prolamin nanoparticles. Res-loaded gliadin (Gli), zein, deamidated gliadin (DG) and deamidated zein (DZ) nanoparticles were filled in WSG. The hardness, G′ and G′′ of WSG were notably increased. It can be attributed to the more ordered and stable structure induced by the interaction of prolamin nanoparticles and starch. The Res retention of nanoparticles and nanoparticle-filled starch gels was at least 24.6 % and 36.0 % higher than free Res upon heating. When exposed to ultraviolet, the Res retention was enhanced by over 6.1 % and 37.5 %. The in-vitro digestion demonstrated that the Res releasing percentage for nanoparticle-filled starch gels was 25.8 %–38.7 % lower than nanoparticles in the simulated stomach, and more Res was released in the simulated intestine. This resulted in a higher bioaccessibility of 82.1 %–93.2 %. The bioaccessibility of Res in Gli/Res/WSG and DG/Res/WSG was greater than that of Zein/Res/WSG and DZ/Res/WSG. More hydrophobic interactions occurred between Res and Gli, DG. The interactions between Res and zein, DZ were mainly hydrogen bonding. The microstructure showed that nanoparticles exhibited dense spherical structures and were uniformly embedded in the pores of starch gels.
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Food Bioscience
·
2024
·
IF 4.8
Caiying Yang,Ying Zhang,Xiaoya Zhou,Zhihua Wu,Jinyan Gao,Hongbing Chen,Ping Tong
Allergen modification is a novel strategy for preventing and treating food allergy. Resveratrol (RES) can be used as a modifying ligand for allergens, but the interaction mechanism between RES and allergens is still not detailed, and the ramifications of this interaction on the structure, epitope and allergenicity of allergens are also lacking a clear explanation. In addition, the application of heat can promote protein structure to unfold and expose more binding sites to facilitate interaction. Therefore, in this study, ovalbumin (OVA) was selected as the allergen model and was heated to expose more binding sites. The effects of the interaction between RES and heat-stressed ovalbumin (HOVA) on allergen structure, epitope and allergenicity were investigated by combining routine experimental analysis and computer simulation. Results showed heat stress at 323 K for 15 min could better unfold the molecular structure of OVA. On this basis, the interaction between RES and OVA was strengthened, which showed a static quenching of the ground state complex with binding affinity of 1.25 × 10 6 L/mol. The binding of RES induced the variation of protein microenvironment, and the overall structure tended to disordered direction. Computer simulation had shown RES could directly act on the IgE epitope region (AA251-260) of the OVA. The allergenicity evaluation experiment further indicated the combination of RES alleviated the IgE binding capacity of HOVA. These findings are helpful to further study the mechanism of RES ligand modification to change food allergy.
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REACTIVE & FUNCTIONAL POLYMERS
·
2025
·
IF 5.1
Wenxiu Chen,Youzhi Zeng,Zaoxia Wen,Linxuan Fang,Lianbin Wu
A trifunctional methacrylate monomer (REM) was synthesized from biomass resveratrol to address the need for sustainable resins in digital light processing (DLP) 3D printing. The polymer properties were optimized by incorporating methacryloxypropyl polyhedral oligomeric silsesquioxane (MA-POSS) with different mass fractions. Light-curing biobased materials (REM/POSS) exhibited enhanced thermal stability and dielectric properties. The REM/POSS-30 % composite showed a low dielectric constant ( D k = 2.90 at 14.2 GHz) and excellent thermal stability. Compared to commercial bisphenol A-based resins (MEP), REM/POSS-0 % demonstrated superior comprehensive performance. The stilbene structure of resveratrol in REM/POSS polymers provided strong blue fluorescence under UV light, suggesting potential for information storage and encryption. The successful fabrication of complex structures confirmed the compatibility of REM/POSS with DLP 3D printing, offering a promising alternative to petroleum-based materials in both 3D printing and anti-counterfeiting applications.
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