BIORESOURCE TECHNOLOGY
·
2014
·
IF 5.039
Yu-Cai He,Zhi-Cheng Tao,Xian Zhang,Zhen-Xing Yang,Jian-He Xu
An NADH-dependent reductase (CmCR) from Candida magnoliae was discovered by genome mining for carbonyl reductases. After CmCR was overexpressed in Escherichia coli BL21, a robust reductase-producing strain, recombinant E. coli CCZU-K14, was employed for the efficient synthesis of ethyl ( S )-4-chloro-3-hydroxybutanoate (( S )-CHBE) from the reduction of ethyl 4-chloro-3-oxobutanoate (COBE). After the optimization, the optimum reaction conditions were obtained. Notably, E. coli CCZU-K14 had broad substrate specificity in reducing both aliphatic and aromatic substrates, and excellent enantioselectivity of CCZU-K14 was observed for most of the tested substrates, resulting in chiral alcohols of over 99.9% ee . Moreover, COBE at a high concentration of (3000 mM) could be asymmetrically reduced to ( S )-CHBE in the high yield (>99.0%) and high enantiometric excess value (>99.9% ee ) after 14 h. Significantly, E. coli CCZU-K14 shows high potential in the industrial production of ( S )-CHBE and its derivatives (>99.9% ee ).
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 2.892
Ya-Jun Wang,Xiao-Peng Chen,Wei Shen,Zhi-Qiang Liu,Yu-Guo Zheng
In our previous work, we constructed a carbonyl reductase (CR) and glucose dehydrogenase (GDH) co-expression strain E. coli BL21(DE3)/pCDFDuet- gdh - cr , and a biocatalytic process of chiral diol t -butyl 6-cyano-(3 R ,5 R )-dihydroxyhexanoate ((3 R ,5 R )- 2 ) was established by using its free cells. Herein, a (3 R ,5 R )- 2 process with celite-polyethyleneimine (PEI)-glutaraldehyde (GA) immobilized E. coli BL21(DE3)/pCDFDuet- gdh - cr whole cells was developed for the first time. It was demonstrated that immobilization via cross-linking was preferred, and the optimized immobilization conditions were as follows: 0.6% (w/v) celite, 4% (w/v) PEI and 1% (w/v) GA, pH7. The immobilizate can be used at pH 7.0, 30 °C for intermittent 7 batch, yielding a total of 17.5 g (3 R ,5 R )- 2 per gram of celite-PEI-GAimmobilizates in de p (diastereomeric excess) >99.5%. Compared with the free cells, there was a dramatic improvement in celite- PEI-GA immobilizate stability and recyclability. In this way, a promising platform technology based on a combination of cells immobilization and multiple enzymes co-expression is constructed, enabling efficiently converting inexpensive prochiral ketones to high-valued chiral alcohols.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Deshou Wang,Yumeng Liang,Yong Zeng,Chang Liu,Chun Zhan,Peng Chen,Shaoxian Song,Feifei Jia
The recycling of electronic waste, i.e ., waste Printed Circuit Boards (WPCBs), provides substantial environmental and economic advantages. In fact, the concentration of valuable precious and base metals in WPCBs is even higher compared to those found in mined ores. Nevertheless, it is still challenging to selectively extract precious metals with low concentrations from the pregnant leaching solution, due to the co-deposition of base metals, like Cu, which have higher concentrations. In this research, stepwise recovery of precious metals and copper directly from WPCBs thiosulfate leaching solution was facilitated by the Ti cathode coated with MoS 2 (MoS 2 /Ti). The in-situ enrichment of Au(S 2 O 3 ) 2 3- and Ag(S 2 O 3 ) 2 3- at the surface of MoS 2 enables the high efficiency and selectivity of electrodeposition , which has been confirmed through COMSOL Multiphysics simulations and visualization. As a result, the first-step electrodeposition at 0.6 V recovered 92.44 % Au and 98.18 % Ag without any co-deposition of Cu. Subsequently, the second-step recovery employed a constant current of 0.03 A, achieving 100 % recovery of copper within 12 h. Furthermore, this study optimized the reduction potential, NH 3 ·H 2 O concentration, and S 2 O 3 2- concentration for the stepwise electrodeposition process. These findings provide valuable insights for establishing a closed loop circular economy in the electronics industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2024
·
IF 3.6
Zhiqi Gu,Hui Chang,Guo Yang,Boxuan Xu,Bin Miao,Jiadong Li
Vertical flow assay (VFA) is an effective point-of-care (POC) diagnostic tool for widespread applications. Nevertheless, the lack of multi-target detection and multi-signal readout capability still remain challenges. Herein, a brand new VFA scheme for multi-target saliva detection based on electronic-tags was proposed, where AlGaN/GaN HEMT sensors modified with different bio-receptors as electronic tags endowed the VFA a multi-target detection capability. In addition, the use of electronic tags instead of optical tags made that the VFA could simultaneously carry out direct multi-target readout, which ensure the effective POC diagnostic of saliva analysis. Moreover, by integrating a hydrophilically optimized micro-sieve, impurities like sticky filaments, epidermal cells and other large-scale charged particles in saliva were effectively screened, which enabled the direct detection of saliva by AlGaN/GaN HEMT sensors. Glucose, urea, and cortisol were selected to verified the feasibility of the multi-target e-VFA scheme, results displayed that the limit-of-detection (LOD) was as low as 100 aM. The linear response was demonstrated in the dynamic range of 100 aM - 100 μM, the specificity and long-term stability and the validity of the actual saliva test were also verified. These results demonstrated that the as-proposed e-VFA had potential application in saliva detection for multi-target simultaneous detection, and it is expected to achieve the real-time detection of more biological targets in saliva.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-10
·
IF 5.3
Wang Panxue,Liu Ying,Li Xiang,Li Cen,Li Guoliang
A ratiometric SERS aptasensor based on catalytic hairpin self-assembly (CHA) mediated cyclic signal amplification strategy was developed for the rapid and reliable determination of Escherichia coli O157:H7. The recognition probe was synthesized by modifying magnetic beads with blocked aptamers, and the SERS probe was constructed by functionalizing gold nanoparticles (Au NPs) with hairpin structured DNA and 4-mercaptobenzonitrile (4-MBN). The recognition probe captured E. coli O157:H7 specifically and released the blocker DNA, which activated the CHA reaction on the SERS probe and turned on the SERS signal of 6-carboxyl-x-rhodamine (ROX). Meanwhile, 4-MBN was used as an internal reference to calibrate the matrix interference. Thus, sensitive and reliable determination and quantification of E. coli O157:H7 was established using the ratio of the SERS signal intensities of ROX to 4-MBN. This aptasensor enabled detection of 2.44 × 10 2 CFU/mL of E. coli O157:H7 in approximately 3 h without pre-culture and DNA extraction. In addition, good reliability and excellent reproducibility were observed for the determination of E. coli O157:H7 in spiked water and milk samples. This study offered a new solution for the design of rapid, sensitive, and reliable SERS aptasensors. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WASTE MANAGEMENT
·
2025
·
IF 7.1
Ke Xu,Shengde Dong,Chunxi Hai,Luxiang Ma,Yanxia Sun,Qi Xu,Xin He,Yuan Zhou
The recycling of cathode materials in waste lithium-ion batteries is of great significance to resource utilization and environmental protection. However, few studies have addressed the challenges posed by high-aluminum LiFePO 4 black powder (HALFP-BP) resulting from inadequate pre-treatment. This study proposes a low-temperature short-time sulfuric acid leaching method based on thermodynamic analysis of E-pH diagrams. Under optimized conditions, the leaching efficiencies of Li and Fe reached 99 %, while 86 % of Al was selectively removed. Furthermore, the guiding role of E-pH diagram in the recovery process and the chemical reactions and Gibbs free energy analysis during the acid leaching process were analyzed. It revealed that the dissolution kinetics inhibition of the dense Al 2 O 3 film on the surface of aluminum impurities in the sulfuric acid system was the key to achieving selective separation. This method provides an energy-saving and efficient solution for the resource utilization of the HALFP-BP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Mingzhu Zhang,Dongfeng Guo,Guanglong Wu,Ping Han,Yaqi Shi,Tianfei Zheng,Xiaohui He,Eryong Zhao,Hui Zhang,Xingjiang Li
Tobacco is an agricultural commodity with great economic significance. Producers recognize that the flavor characteristics of filler tobacco clear-out (FTLs) affect the quality of cigar tobacco items. To identify the major volatile organic compounds (VOCs) responsible for differences in FTLs, E-nose, HS-SPME-GC–MS, and HS-GC-IMS were employed for multi-dimensional analysis of nine groups of FTLs at various fermentation stages. The E-nose results indicated a progressive increase in nitrogen-oxygen compounds during fermentation. Coupled with these findings, HS-SPME-GC–MS identified 56 VOCs, and HS-GC-IMS revealed 55 VOCs in the FTLs. Principal component analysis (PCA) suggested that FTLs from various fermentation stages of the Yunxue No 39 tobacco plant exhibit significant differences. Partial least squares discriminant analysis (PLS-DA), along with the variable importance in projection (VIP) method for feature selection, pinpointed 12 VOCs as the essential unmistakable metabolites embroiled within the agrarian fermenting prepare of matured FTLs. During the fermentation process, these crucial metabolites are predominantly enriched in pathways including pyruvate metabolism, phenylalanine metabolism, as well as nicotinate and nicotinamide metabolism. The study provides insights into the differences and diversity of FTLs at agricultural fermentation stages. It offers valuable information for optimizing the quality of agricultural fermentation and guiding the directional fermentation of tobacco leaves.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2017
·
IF 3.649
Xiaofeng Han,Xuerong Dong,Jing Li,Manyuan Wang,Lei Luo,Zhaoxia Li,Xuran Lu,Rui He,Rongsong Xu,Muxin Gong
The present work seeks to construct a nanovehicle for the efficient suppression of breast cancer metastasis through targeting E-selectin on tumor vascular endothelial cells and hyaluronic acid-receptor on tumor cells. Herein, a new ligand-PEG-lipid conjugate, E-selectin binding peptide-polyethene glycol-1-octadecylamine (Esbp-PEG-OA), was used as the targeting molecule of micelle self-assembled form hyaluronic acid-paclitaxel (HA-PTX) conjugate. When loaded with free PTX, the micelles (Esbp-HA-PTX/PTX) exhibited nanoscale particle size with high drug-loading capacity (up to 31.5%). In vitro release study showed that the conjugated and entrapped PTX released simultaneously. Cellular uptake of micelles confirmed that Esbp-HA-PTX micelles could be specifically and efficiently internalized into E-selectin expressing human umbilical vein endothelial cells (HUVEC) and 4T1 breast cancer cells via receptor-meditated endocytosis. In vitro cytotoxicity assay further revealed that Esbp-HA-PTX/PTX micelles significantly improved the selectivity of PTX for killing the two cell types compared with PTX solution formulation. More importantly, Esbp-HA-PTX micelles raised the accumulation of payload in tumor through targeting two cell types in the tumor microenvironment simultaneously, resulting in marked in vivo inhibition of tumor growth, intratumoral microvessel density and metastasis, and decreased systemic toxicity over solution formulation. Overall, Esbp-HA-PTX/PTX micelle is promising in therapy of breast cancer metastasis.
✓ 已验证
DOI
PubMed
高影响因子
Materials Research Express
·
2021
·Vol. 8
·期 12
·125006
·
IF 1.62
Peng She,Chun Yao,Jun Li,Hegang Bao,Xiuquan Xu,Hong Zhou
Co-doping is one of the most facile and efficient strategies for boosting the photocatalytic performance of g-C3N4. Herein, a series of Ti and O co-doped g-C3N4 (Ti/OCN) composites with excellent photocatalytic inactivation activity of Enterobacter cloacae (E. cloacae) were successfully synthesized by simple calcination of urea/formic acid/TiCl4 precursor. The obtained Ti/OCN-3 possessed the optimal photocatalytic capability with complete inactivation of 6.20 log CFU·ml−1 E. cloacae under 60 min of visible light irradiation. The improved photocatalytic disinfection performance was probably attributed to the synergistic effect of Ti and O co-doping, which led to an extended visible light response and accelerated charge transport efficiency. Furthermore, scavenger trapping and electron spin resonance (ESR) experiments revealed that ·O2−, h+ and ·OH were responsible for the enhanced inactivation activity. Overall, this research provided a promising strategy for fabricating g-C3N4-based catalysts to efficiently deal with the hazards of pathogenic microorganisms.
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
He-Lin Xu,Zi-Liang Fan,De-Li ZhuGe,Bi-Xin Shen,Bing-Hui Jin,Jian Xiao,Cui-Tao Lu,Ying-Zheng Zhao
Severe toxicity and poor tumour penetration are two intrinsic limited factors to hinder the broad clinical application for most of first-line chemotherapeutics. In this study, a novel vitamin E succinate-grafted ε-polylysine (VES-g-PLL) polymer was synthesized by using ε-polylysine as backbone. By adjusting VES graft ratio, VES-g-PLL (50) with a theoretic VES graft ratio of 50% could self-assemble into a supermolecular micelle with a hydrodynamic diameter (D h ) of ca.20 nm, and Zeta potential of 19.6 mV. VES-g-PLL micelles themselves displayed a strong anti-tumour effect on glioma. The poorly water-soluble curcumin was effectively encapsulated in VES-g-PLL micelles with the drug loading amount and entrapment efficiency reaching 4.32% and 82.27%, respectively. In a physiologic medium, curcumin-loaded VES-g-PLL micelles (Cur-Micelles) not only remained stable without obvious drug leakage but also sustained the release of its encapsulated curcumin for a long time. Because of the ultra-small size and positively-charged surface, Cur-Micelles penetrated the deeper tumour zone than free curcumin, resulting in a significant inhibition of tumour spheroids growth. Moreover, in vivo strong antitumor effect of Cur-Micelles was also exhibited at assistance of ultrasound-targeted microbubble destruction and the real-time MRI imaging demonstrated a nearly complete suppression of glioma after 28 days of treatment. TUNEL staining showed that the therapeutic mechanism of Cur-Micelles was relevant to the apoptosis of tumour cells. Finally, in vivo nontoxicity of Cur-Micelles against normal organs including heart, liver, spleen, lung and kidney tissues was also demonstrated by the HE staining. In conclusion, VES-g-PLL micelles may serve as a potential carrier for curcumin to enhance tumour penetration and improve therapeutic effect on glioma.
✓ 已验证
DOI
高影响因子
Nutrients
·
2024
·Vol. 16
·期 10
·1468
·
IF 4.8
Yan Zhang,Hong Huang,Chuanlin Luo,Xinfeng Zhang,Yanjing Chen,Fenfang Yue,Bingqing Xie,Tingtao Chen,Changwei Zou
Cadmium (Cd) is recognized as being linked to several liver diseases. Currently, due to the limited spectrum of drugs available for the treatment of Cd intoxication, developing and designing antidotes with superior detoxification capacity and revealing their underlying mechanisms remains a major challenge. Therefore, we developed the first next-generation probioticE. coli 1917-pSK18a-MT that delivers metallothionein (MT) to overcome Cd-induced liver injury in C57BL/6 mice by utilizing bacterial surface display technology. The results demonstrate thatE. coli 1917-pSK18a-MT could efficiently express MT without altering the growth and probiotic properties of the strain. Moreover, we found thatE. coli 1917-pSK18a-MT ameliorated Cd contamination-induced hepatic steatosis, inflammatory cell infiltration, and liver fibrosis by decreasing the expression of aminotransferases along with inflammatory factors. Activation of the Nrf2-Keap1 signaling pathway also further illustrated the hepatoprotective effects of the engineered bacteria. Finally, we showed thatE. coli 1917-pSK18a-MT improved the colonic barrier function impaired by Cd induction and ameliorated intestinal flora dysbiosis in Cd-poisoned mice by increasing the relative abundance of the Verrucomicrobiota. These data revealed that the combination ofE. coli 1917and MT both alleviated Cd-induced liver injury to a greater extent and restored the integrity of colonic epithelial tissues and bacterial dysbiosis.
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jinghe Sun,Weiyun Zheng,Xiaoyan Wang,Yimeng Li,Jingfeng Yang
The freshness in A. japonicus after thymol treatment was tested. The results showed that sensory scores in thymol group significantly increased 14.83 and 21.67 compared to the Frozen and BK groups after storage. Texture parameters showed that hardness, springiness, and chewiness of body wall treated with thymol were 2.56, 1.21, and 2.02 folds that of the BK group, respectively. Volatile flavor results revealed 38 flavor substances and 33 volatile organic compounds in the body wall. Aldehydes, alcohols, heterocycles, and alkane were the key sources to odor components of A. japonicus , including nonanal, hexanal, 1-octanol, 1-hexanol, d -limonene, and 2-methylbutyl acetate. Moreover, heptenoic acid, dimethyl disulfide, and dimethylamine, as distinct volatile flavor substances, were the main contributors of fishy, malodorous, and spicy smells of autolysis A. japonicus . This study guides the potential use of thymol in sea cucumber preservation and seeks the spoiled flavor markers for non-destructive rapid testing technology development.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）