CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Jun-Sheng Liu,Yue-Xiang Zeng,Shi-Yuan Bi,Jin-Wei Zhou,Rui Cheng,Jing Li,Ai-Qun Jia
Phomopsis liquidambari is a classical endophytic fungus with great application potential in ecology and agriculture; however, studies on its exopolysaccharides are lacking. Here, we aimed to evaluate the structure and bioactivity of PLN-1, an exopolysaccharide derived from the P. liquidambari NJUSTb1 strain. The structure was elucidated by chromatography/spectral methods and hydrolyzation. Immunomodulation, moisture absorption, and retention properties were investigated after sulfation and carboxymethylation modification. Results showed that PLN-1 contained a linear repeating unit of →[4)- α - d -Glc p -(1→6)- α - d -Glc p -(1→4)- α - d -Glc p -(1→4)- α - d -Glc p -(1→] n , with a molecular weight of 343 kDa. The degrees of substitution of sulfated polysaccharide (S-PLN-1) and carboxymethylated polysaccharide (C-PLN-1) were 1.228 and 0.903, respectively. S-PLN-1 showed stronger moisture absorption and retention properties than PLN (crude EPS), C-PLN1, and PLN-1. Furthermore, PLN, S-PLN-1, and C-PLN-1 stimulated the proliferation of RAW 264.7 cells with no cytotoxicity. The elucidation of PLN-1 in this study paves the way for future applications.
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高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2025
·
IF 3.5
Huanxu Teng,Meihua Zhu,Yixuan Shao,Siqi Shao,Qingsheng Wu,Ziming Li,Xiangshu Chen,Hidetoshi Kita
Supported TS-1 zeolites were successfully synthesized on α-Al 2 O 3 microsphere by secondary hydrothermal synthesis, which were placed in the fixed-bed reactor and catalyzed the oxidation of isopropanol(IPA) with hydrogen peroxide. Effects of synthesis parameters on the morphology and catalytic activity of the supported TS-1 zeolites were systematically studied in this work. Size of α-Al 2 O 3 microsphere, and crystallization temperature could determine the coverage, titanium coordination, and titanium content of the supported TS-1 zeolites. When the crystallization temperature and H 2 O/SiO 2 molar ratio of the initial synthesis solution were 150 °C and 120, the titanium-rich and anatase-free supported TS-1 zeolites were prepared on the ca. 1 mm α-Al 2 O 3 microsphere support. The supported TS-1 zeolites showed an excellent catalytic performance and stability for isopropanol oxidation, the isopropanol conversion and acetone selectivity reached 100 % within 2 h. Kinetic studies showed that the reaction conformed to the Langmuir-Hinshelwood mechanism and that the apparent activation energy was only 4.57 kJ/mol.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yawei Zhao,Jing Zhang,Xiaohan Fan,Yue Cai,Wenting Du,Ziyi Yu,Biao Xu,Xuan Sun
Microvascular obstruction (MVO) is recognized as an independent risk factor for adverse prognosis in patients with myocardial infarction (MI), and its development is mechanistically linked to inflammation and microthrombus. Maresin 1 (MaR1), a specialized pro-resolving mediator, has demonstrated therapeutic potential in counteracting these processes, but its translation has been hindered by poor stability and rapid degradation. To overcome these limitations, we report the development of a cardiac-targeted liposomal nanotherapeutic (nano-MaR1) designed to stabilize and selectively deliver MaR1 to the injured myocardium. Nano-MaR1 was generated through a scalable nanoprecipitation method in a herringbone microfluidic mixer, with MaR1 encapsulated by a freeze–thaw approach to minimize degradation, and further modified with a cardiac-homing peptide for organ-specific targeting. In a murine MI model, intravenous administration of nano-MaR1 led to rapid myocardial enrichment within 12 h, with 20 μM identified as the optimal therapeutic dose. Treatment markedly reduced infarct size, cardiomyocyte apoptosis, inflammatory infiltration, and MVO formation, while promoting reparative macrophage polarization toward the M2 phenotype. Mechanistic studies revealed that these effects were mediated by downregulation of Itgam (CD11b) within the coagulation cascade and inhibition of monocyte–platelet aggregate formation, which were abolished upon CD11b blockade. Collectively, this work establishes cardiac-targeted liposomal encapsulation as a powerful strategy to enhance the stability and bioactivity of MaR1, and highlights nano-MaR1 as a promising therapeutic candidate for mitigating MVO and improving post-MI repair.
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高影响因子
Evidence-based Complementary and Alternative Medicine
·
2022
·
IF 2.65
Hong Zhang,Jian Ye,Xu Wang,Zongjun Liu,Tao Chen,Junqing Gao
The inhibitory effect of muscone on the hyperinflammatory response after myocardial ischemia reperfusion injury (MIRI) was investigated, and the target and signal pathways of muscone were explored. The levels of inflammatory cytokines interleukin-1β, interleukin-6, and tumor necrosis factor alpha were detected through qRT-PCR and ELISA. The expression levels of p38 and NF-κB signaling pathway-related proteins were detected through Western blot. TREM-1 siRNA was transfected into macrophages in vitro. The rat model of myocardial ischemia was established and used in studying the inhibitory effect of muscone on the inflammatory response and its protective effect muscone on myocardial apoptosis. The expression of TREM-1 was upregulated during myocardial ischemia. Knocking down TREM-1 decreased the increase in inflammatory cytokines in the supernatant of macrophages induced by rmHMGB1 (1 μg/mL) and rmHSP60 (1 mol/mL). In addition, knocking down TREM-1 decreased p38 and NF-κB signaling activation. Muscone can protect myocardial cells by inhibiting the expression of TREM-1 and the inflammatory response after myocardial infarction. Further study showed that muscone inhibited the production of DAM-triggered (damage-associated molecular pattern trigger) inflammatory cytokines. In addition, muscone inhibited the activation of p38 and NF-κB signals under DAM-induced conditions. Muscone and TREM-1 gene knockout reduced cell apoptosis and provided protection against MIRI by inhibiting p38 and NF-κB signaling activation. Mechanism studies showed that muscone inhibited the production and release of inflammatory cytokines by inhibiting TREM-1, and thereby reducing the inflammatory response and providing protection against MIRI.
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高影响因子
CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 19
·期 1
·e202100694
·
IF 2.408
Zhipeng Qi,Xinyi Tong,Su Bu,Jianjun Pei,Linguo Zhao
Natural β-ionone, a high-value flavoring agent, has been widely applied in the food, cosmetics, and perfume industry. However, attempts to overproduce β-ionone in microorganisms have been limited by the efficiency of carotenoid cleavage dioxygenases (CCDs), which catalyzes β-carotene in the biosynthesis pathway. In order to obtain CCD genes responsible for the specific cleavage of carotenoids generating β-ionone, a novel carotenoid cleavage dioxygenase 1 from Helianthus annuus was cloned and overexpressed in Escherichia coli BL21(DE3). The recombinant CCD was able to cleave a variety of carotenoids at the 9, 10 (9′, 10′) sites to produce C13 products in vitro , including β-ionone, pseudoionone, 3-hydroxy-4-oxo-β-ionone, 3-hydroxy-β-ionone, and 3-hydroxy-α-ionone, which vary depending on the carotenoid substrates. In comparison with lycopene and zeaxanthin, HaCCD1 also showed the high specificity for β-carotene to cleave the 9, 10 (9′, 10′) double bond to produce β-ionone in E. coli accumulating carotenoids. Finally, the expression of HaCCD1 in E. coli was optimized, and biochemical characterizations were further clarified. The optimal activity of HaCCD1 was at pH 8.8 and 50 °C. The V max for β-apo-8′-carotenal was 10.14 U/mg, while the K m was 0.32 mM. Collectively, our study provides a valuable enzyme for the synthesis of natural β-ionone by biotransformation and synthetic biology platform.
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高影响因子
Inorganics
·
2025
·Vol. 13
·期 2
·37
·
IF 3
Yao Wang,Hongda Yu,Huan Wang,Tiehong Chen
The environmental and health impacts of sulfur compounds in fuel oil have prompted considerable research interest in oxidative desulfurization (ODS) technology. Tetrahedrally coordinated titanium has been demonstrated to exhibit excellent activity in the context of oxidative desulfurization processes. However, further improving the catalytic property of the tetrahedrally coordinated titanium remains a challenging endeavor. In the context of ODS processes conducted at near room temperatures, the improvement of conversion remains a subject of considerable challenge. In this study, hierarchically porous titanosilicate hollow spheres were synthesized by using TS-1 zeolite precursors as Ti and Si sources to obtain the catalyst with only tetrahedrally coordinated titanium. The synthesized materials were characterized through transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), ultraviolet–visible diffuse reflectance spectroscopy (UV-Vis), and nitrogen adsorption analysis. These techniques confirmed the formation of hollow spherical hierarchically porous structures with Ti species uniformly incorporated in tetrahedral coordination and the presence of five-member rings of TS-1 zeolite. As a result, the hierarchically porous titanosilicate hollow spheres demonstrated excellent catalytic performance in ODS, achieving complete dibenzothiophene (DBT) removal within 15 min and a high turnover frequency (TOF) of up to 123 h−1at 30 °C.
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高影响因子
CURRENT PHARMACEUTICAL DESIGN
·
2017
·Vol. 23
·期 39
·6071-6078
·
IF 2.611
Hua Wenbin,Zhang Yukun,Wu Xinghuo,Kang Liang,Tu Ji,Zhao Kangcheng,Li Shuai,Wang Kun,Song Yu,Luo Rongjin,Shao Zengwu,Yang Shuhua,Yang Cao
Background: Low back pain is a common problem, mainly caused by intervertebral disc degeneration (IDD). An important pathophysiological characteristic of IDD is the loss of homeostatic balance of the extracellular matrix metabolism. Interleukin-1β (IL-1β) is one of the inflammatory
mediators stimulating the degradation of extracellular matrix in the nucleus pulposus (NP) and contributing to IDD pathogenesis. Icariin, which is isolated from Epimedium brevicornum, acts as an anti-inflammatory drug. Objective: This study aimed to explore the pharmacological effects
of icariin in IDD by simulating NP inflammation in vitro. Method: Human NP cells were isolated and cultured in vitro. NP cells were pretreated with icariin (0.1, 1 and 10 μM) and stimulated by IL-1β (10 ng/ml). The concentration of Prostaglandin E2 was determined by enzymelinked
immunosorbent assay. Nitric oxide was determined by Griess reagent assay. The expression of cyclooxygenase- 2 (COX-2), inducible nitric oxide synthase (iNOS), degrading enzymes, collagen II, aggrecan, mitogenactivated protein kinase (MAPK), and nuclear factor-kappa B (NF-ΚB)-related signaling
molecules was assessed via western blotting. Results: IL-1β induced pronounced expression of COX-2 and iNOS, and stimulated production of prostaglandin E2 and nitric oxide. Icariin exhibited significant anti-inflammatory effect, inhibiting IL-1β-induced production of degrading
enzymes, as well as extracellular matrix reduction. Finally, icariin suppressed IL-1β-induced activation of MAPK- and NF-ΚB-related signaling pathways. Conclusion: The present findings suggest that icariin may have a protective effect on NP cells. The antiinflammatory effect
may contribute to the therapeutic action of icariin in IDD.
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高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 47
·475708
·
IF 3.874
Qiu-Ping He,Yun Zou,Peng-Fei Wang,Xiao-ming Dou
Silicalite-1 zeolitic membranes have been successfully fabricated on the porous α-Al2O3 support by templated and template-free protocol, respectively, for vapor permeation separation of dichlorobenzene (DCB) isomers. After proving the high quality of the membranes by single gas permeation (He and SF6) performance, the vapor-permeation of DCB isomers over two types of the silicalite-1 membrane was then investigated. The separation results clearly indicated that under the lower partial pressure and higher temperature, the effect of DCB isomer adsorption on the permeance could be kept at a sufficiently low level and sharp selectivity become more important. Thus, high p-DCB selectivity could be achieved. Comparatively, the template-free silicalite-1 zeolite has a much higher p-DCB selectivity due to the relatively fewer inter-crystalline gaps. Under certain separation conditions, the highest selectivity of p-DCB for p-/m-DCB and p-/o-DCB binary systems could reach 165 and 113, respectively.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Guojun Lv,Tao Wang,Yan Chen,Jiangzhang Wang,Xuyang Zou,Jun Lu,Fumin Wang,Xubin Zhang,Yi Zhai
Design of catalyst particles bearing excellent diffusion performance and suitable surface wettability is of great importance to the successful application of Pickering interfacial catalysis. In this study, selective oxidation reactions over hydrophobized hollow TS-1 zeolite were applied as probe reactions to investigate the influence of catalyst diffusion performance closely related to mesopore size on its catalytic activity. Hydrophobized hollow TS-1 zeolite particles were prepared via a post desilication treatment accompanied with precrystallization treatment. The synthesized hydrophobized hollow TS-1 zeolite was characterized by Scanning electron microscopy, Transmission electron microscopy , X-ray diffraction, UV–vis absorption spectrometry , Fourier-transform infrared spectroscopy and Nitrogen adsorption-desorption. Stable Pickering emulsions including 1-hexene-hydrogen peroxide, cyclohexene-hydrogen peroxide and cyclohexanone-water were prepared with hydrophobized hollow TS-1 zeolite particles as emulsifier . HTS-1–393(2)-S zeolite synthesized with precrystallization at 393 K for 2 h presented the maximum values of 43.9 mol/(Ti-mol·h) for 1-hexene epoxidation , 15.7 mol/(Ti-mol·h) for cyclohexene epoxidation and 247.3 mol/(Ti-mol·h) for cyclohexanone ammoximation. In addition, HTS-1–393(2)-S zeolite particles displayed good reusability for more than five cycles.
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高影响因子
FIBERS AND POLYMERS
·
2016
·Vol. 17
·期 11
·1741-1748
·
IF 1.022
Xu Bin,Li Qiaoping,Zhuang Linghua,Wang Qiang,Li Chao,Wang Guowei,Xie Fengwei,Halley Peter J.
3-methyl-1-(ethylacetyl)imidazolium chloride ([EtMIM]Cl), was synthesized for chitosan dissolution, and the dissolution and regeneration behaviors of chitosan in [EtMIM]Cl were thoroughly investigated. The solubility of chitosan in [EtMIM]Cl was measured at temperatures ranging from 40 °C to 110 °C, based on which the thermodynamic parameters of chitosan in [EtMIM]Cl were calculated. The polarizability and hydrogen bond accepting ability was determined by solvatochromic UV/vis spectroscopy. The regenerated chitosan from [EtMIM]Cl by adding methanol was characterized by thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM). Density functional theory (DFT) computations were performed to study the interactions between [EtMIM]Cl and chitobiose. Five kinds of hydrogen bonds, C-H/O, O-H/O, O-H/Cl, C-H/Cl, N-H/Cl were found, suggesting strong interactions between [EtMIM]Cl and chitobiose. In particular, the oxygen atom and the active methylene group of carboxylic ester in [EtMIM] + , formed strong hydrogen bonding with chitobiose. The molecular simulation results indicated that both the Cl − anions and [EtMIM] + cation played important roles in the chitosan dissolution process, by the disruption of native hydrogen bonds of chitosan.
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高影响因子
Microbial Cell Factories
·
2024
·Vol. 23
·期 1
·1-16
·
IF 4.3
Wang Yifan,Shu Dan,Li Zhemin,Luo Di,Yang Jie,Chen Dongbo,Li Tianfu,Hou Xiaonan,Yang Qi,Tan Hong
Background Currently, industrial fermentation of Botrytis cinerea is a significant source of abscisic acid (ABA). The crucial role of ABA in plants and its wide range of applications in agricultural production have resulted in the constant discovery of new derivatives and analogues. While modifying the ABA synthesis pathway of existing strains to produce ABA derivatives is a viable option, it is hindered by the limited synthesis capacity of these strains, which hinders further development and application. Results In this study, we knocked out the bcaba4 gene of B. cinerea TB-31 to obtain the 1′,4′- trans -ABA-diol producing strain ZX2. We then studied the fermentation broth of the batch-fed fermentation of the ZX2 strain using metabolomic analysis. The results showed significant accumulation of 3-hydroxy-3-methylglutaric acid, mevalonic acid, and mevalonolactone during the fermentation process, indicating potential rate-limiting steps in the 1′,4′- trans -ABA-diol synthesis pathway. This may be hindering the flow of the synthetic pathway. Additionally, analysis of the transcript levels of terpene synthesis pathway genes in this strain revealed a correlation between the bchmgr , bcerg12 , and bcaba1-3 genes and 1′,4′- trans -ABA-diol synthesis. To further increase the yield of 1′,4′- trans -ABA-diol, we constructed a pCBg418 plasmid suitable for the Agrobacterium tumefaciens -mediated transformation (ATMT) system and transformed it to obtain a single-gene overexpression strain. We found that overexpression of bchmgr , bcerg12 , bcaba1 , bcaba2 , and bcaba3 genes increased the yield of 1′,4′- trans -ABA-diol. The highest yielding ZX2 A3 strain was eventually screened, which produced a 1′,4′- trans -ABA-diol concentration of 7.96 mg/g DCW (54.4 mg/L) in 144 h of shake flask fermentation. This represents a 2.1-fold increase compared to the ZX2 strain. Conclusions We utilized metabolic engineering techniques to alter the ABA-synthesizing strain B. cinerea , resulting in the creation of the mutant strain ZX2, which has the ability to produce 1′,4′- trans -ABA-diol. By overexpressing the crucial genes involved in the 1′,4′- trans -ABA-diol synthesis pathway in ZX2, we observed a substantial increase in the production of 1′,4′- trans -ABA-diol.
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2023
·
IF 5.2
Zilin Wang,Tao Wang,Long Mu,Zhilin Chen,Lishuang Hu,Chao Xiao
Understanding of the main factors affecting the hydrothermal stability of zeolites is vital for the application of zeolites in catalysis and separation. Although incorporation of extra-framework metal cations is an important stagey to improve the hydrothermal stability of Al-rich zeolites, how these metal cations affect the hydrothermal stability of high-silica zeolites is not well understood. In this work, the effects of residual alkali cations on the hydrothermal/thermal stability of silicalite-1, a pure-silica MFI-type zeolite, is explored. We show that the alkali impurities from commercial tetrapropylammonium hydroxide (TPAOH) are not negligible; the concentration and type of residual alkali cations dictate the hydrothermal/thermal stability of silicalite-1. Above a certain concentration (∼0.3–0.5 wt%), the alkali cations can lead to less silanol defects but much poorer hydrothermal/thermal stability. Below this concentration, the degrading effect is negligible. Removal of the alkali cations by proton exchange improves the stability significantly. Steaming (hydrothermal treatment) has two opposite effects on the silicalite-1 crystal structure: destruction by hydrolysis and perfecting by healing the defects. For silicalite-1 with considerable amounts of alkali cations, the destruction effect predominates; while the perfecting effect predominates for silicalite-1 with low alkali contents.
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高影响因子
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