BRIEFINGS IN BIOINFORMATICS
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2021
·Vol. 22
·期 2
·1378-1386
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IF 11.622
Li Shasha,Liu Wenli,Chen Yangzhen,Wang Liqin,An Wenlin,An Xiaoping,Song Lihua,Tong Yigang,Fan Huahao,Lu Chenyang
Antiviral therapies targeting the pandemic coronavirus disease 2019 (COVID-19) are urgently required. We studied an already-approved botanical drug cepharanthine (CEP) in a cell culture model of GX_P2V, a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-related virus. RNA-sequencing results showed the virus perturbed the expression of multiple genes including those associated with cellular stress responses such as endoplasmic reticulum (ER) stress and heat shock factor 1 (HSF1)-mediated heat shock response, of which heat shock response-related genes and pathways were at the core. CEP was potent to reverse most dysregulated genes and pathways in infected cells including ER stress/unfolded protein response and HSF1-mediated heat shock response. Additionally, single-cell transcriptomes also confirmed that genes of cellular stress responses and autophagy pathways were enriched in several peripheral blood mononuclear cells populations from COVID-19 patients. In summary, this study uncovered the transcriptome of a SARS-CoV-2-related coronavirus infection model and anti-viral activities of CEP, providing evidence for CEP as a promising therapeutic option for SARS-CoV-2 infection.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Xia Li,Jianpeng Li,Sijia Hao,Ailing Han,Yayu Yang,Guozhen Fang,Jifeng Liu,Shuo Wang
Di(2-ethylhexyl) phthalate (DEHP), the most abundantly used plasticizer, was considered to be a hazardous chemical that was difficult to be degraded naturally. In this study, inspired by the “catalytic triad’’ in serine proteases, an enzyme mimic material was developed by combining the proteases’s active sites of serine, histidine and aspartate (S-H-D) with the self-assembling sequence of LKLKLKL and the aromatic group of fluorenylmethyloxycarbonyl (Fmoc). By mixing the monomer of peptides containing separate S, H and D residues with a ratio of 2:1:1, the enzyme mimics were found to co- assemble into nanofibers (Co-HSD) and showed the highest activity towards DEHP degradation because of the synergistic effects of active sites, orderly secondary structure and stable molecular conformation. To further improve ability and applicability, the high active mimetic enzyme was immobilized onto regenerated cellulose (RC) membranes for DEHP degradation in a continuous recycling mode. The RC membranes were first functionalized by the NaIO 4 oxidation method to form aldehyde groups and then conjugated with the enzyme mimics via Schiff-base reaction. As a biocatalytic membrane, this membrane could not only effectively degrade DEHP, but also showed good stability, thus establishing a promising biomaterial for large scale biodegradation of DEHP in water decontamination and liquid food depollution.
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高影响因子
OPTICAL MATERIALS
·
2021
·
IF 3.08
Dechuan Li,Gungping Zhu,Xuelian Zhao
KTb(CO 3 ) 2 :Eu 3+ phosphors with intense stokes emission were synthesized by hydrothermal method . Under the excitation of 368 nm light, the pure KTb(CO 3 ) 2 shows an intense green emission at 543 nm corresponding to the 5 D 4 → 7 F 5 transition of Tb 3+ , about 5 times higher than that of the K + -free Tb 2 O(CO 3 ) 2 phosphor. As the Tb 3+ is partly substituted by Eu 3+ , the luminescence color can be transformed from green to red. The intensity of red emission of KTb(CO 3 ) 2 :Eu 3+ could greatly increase as the contents of Eu 3+ without any concentration quenching. Under the excited by 368 nm near-ultraviolet, the quantum efficiencies of KTb(CO 3 ) 2 and KTb(CO 3 ) 2 :0.3Eu 3+ were 93% and 80%, respectively. The high quantum efficiency shows that KTb(CO 3 ) 2 :Eu 3+ is a useful color-tunable luminescence material under excitation of ultraviolet light in the anti-counterfeiting, lighting and display.
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高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
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2021
·
IF 2.475
Guichen Gong,Jie Liu,Yuexin Han
The structure–property relationship and adsorption mechanism of a novel collector for cassiterite, 2-carboxyethylphenylphosphinic acid (CEPPA), were comprehensively investigated and compared with those of styrene phosphonic acid (SPA) by density functional theory (DFT) calculations and flotation tests. The net Mulliken charge and frontier molecular orbital (FMO) theory study results indicated that CEPPA exhibited stronger electron-donating ability and higher chemical reactivity than SPA. The flotation test results demonstrated that CEPPA had stronger collecting ability for cassiterite in a much wider effective pH range than SPA. The adsorption simulation results confirmed that the CEPPA molecule was adsorbed on cassiterite (110) surface by two hydrogen bonds. CEPPA monoanion and dianion were adsorbed on cassiterite (110) surface by a series of O-Sn bonds, and the interactions between the O atoms in CEPPA phosphinic group with the Sn atoms on cassiterite (110) surface were stronger than those between the O atoms in CEPPA carboxyl group with the Sn atoms on cassiterite (110) surface. The formation of the O-Sn bonds was mainly contributed from the 2p orbital of O atoms and 5s and 5p orbitals of Sn atoms. The various adsorption configurations of CEPPA on cassiterite accounted for the better collecting ability of CEPPA for cassiterite than that of SPA. The quantitative analysis results were in good agreement with the qualitative experimental test results in a previous paper.
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Polymer Chemistry
·
2018
·Vol. 9
·期 36
·4556-4565
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IF 4.927
Ruihong Xie,Yefei Tian,Shaojun Peng,Liren Zhang,Yongzhi Men,Wuli Yang
Stimulus responsive nanogels have great potential in the application of drug delivery. In this work, biodegradable zwitterionic poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) nanogels were fabricated by the reflux precipitation polymerization, which was clean and convenient for the application of drug delivery. The hydrodynamic size of the obtained PMPC nanogels was below 200 nm with a narrow size distribution (polydispersity index below 0.1). Moreover, the nanogels showed excellent protein adsorption resistance and remained stable at high concentrations of salt. Furthermore, the PMPC nanogels exhibited redox-responsive performance, and the degradation rate of the nanogels increased as the concentration of glutathione and the pH value increased. In addition, the nanogels could be fully disrupted into short linear polymers (Mw = 2000 g mol−1) after 24 h degradation. The doxorubicin-loaded nanogels showed low drug leakage under physiological conditions (below 15% in 24 h), while they presented rapid drug release in a reduction environment (above 80% in 24 h). The cell cytotoxicity assays revealed that the blank zwitterionic nanogels were nontoxic to the human lung adenocarcinoma epithelial cells (A549 cells) even at high concentrations (1 mg mL−1), while the DOX-loaded nanogels showed efficient inhibition to the tumor cells.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2018
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IF 3.141
Yan Li,Tao Li,Long-Qiang Xiao,Yue-Fei Zhang
The polycondensation of 2-pyrazolines is presented. The products are oligomers with the five-membered rings of the 2-pyrazoline as the backbone and the reaction is a polycondensation reaction by decomposition of ROH from the hydrogen atom in the −NH– group and the −OR in the ester group. Metal catalysts, reaction periods, and the addition amounts of the catalyst are investigated to optimize the reaction conditions. The products are down-/up-conversion fluorescence molecules that can significantly enhance cell imaging through the synchronized excitation of visible and near-infrared light and may be widely used in other optical fields.
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POLYMER COMPOSITES
·
2018
·Vol. 40
·期 4
·1595-1601
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IF 1.943
Ming-En Fei,Tianshun Xie,Wendi Liu,Renhui Qiu
Bamboo fibers (BFs)-reinforced unsaturated polyester (UPE) composites were prepared by hand lay-up compression molding. The UPE resins were modified with 2-acetoacetoxyethyl methacrylate (AAEM) and designed to improve the flexibility and toughness of the BFs–UPE composites. The results indicated that the toughness of the composites significantly increased as the AAEM usage increased. When compared with the untreated UPE composites, the composites obtained from AAEM modified UPE resins (with a 20:80 weight ratio of AAEM to UPE) had 21.7% higher impact strength. Dynamic mechanical analysis showed that incorporation of AAEM significantly increased the damping parameter and decreased glass transition temperature of the composites due to the introduction of flexible segments of AAEM into the UPE resins. Scanning electron microscopy images of the impact-fractured surfaces of the composites revealed that AAEM modification improved the interfacial adhesion between BFs and UPE matrices. The main mechanism for the increase in the toughness of the composites could be due to the increased ductility of the matrices. POLYM. COMPOS., 40:1595–1601, 2019. © 2018 Society of Plastics Engineers
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CERAMICS INTERNATIONAL
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2019
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IF 3.45
Hengrui Qiu,Shengli An,Xuejiao Sun,Huimin Yang,Yongqiang Zhang,Wenxiu He
Due to the unconventional properties of MWCNTs-GONRs (multiwalled carbon nanotubes-graphene oxide nanoribbons), we have tried to use it as a carbon resource for supercapacitors . MWCNTs-GONRs/Ni(OH) 2 electrode was obtained by hydrothermal method . Velvet α-Ni(OH) 2 was prepared above NF (nickel-foam) loaded with MWCNTs-GONRs. This layered design can effectively promote the diffusion of ions and increase the active site for MWCNTs-GONRs/Ni(OH) 2 electrode, thus enhancing the electrochemical performance. The electrode exhibits extraordinary electrochemical performances in electrochemical testing, such as supernal specific capacitance (1713.2 F g −1 ) and prominent working time. In addition, supercapacitors was assembled with MWCNTs-GONRs/Ni(OH) 2 and active carbon as materials. Which represents a prominent energy density (41.23 Wh kg −1 ), high power (6.80 kW kg −1 ) and prominent cycling stability property (95.18%, 3000 times). The electrode prepared in this work provides a clue to enlighten people for energy storage.
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JOURNAL OF CELLULAR BIOCHEMISTRY
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2018
·Vol. 120
·期 5
·7602-7611
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IF 2.959
Xiaolin Wang,Teng Ma,Xiaojian Wan,Yan Meng,Zhenzhen Zhao,Jinjun Bian,Rui Bao,Xiaoming Deng,Tao Yang
IGFBP7 as an early biomarker has been used to identify patients at risk of developing acute kidney injury (AKI). Nevertheless, its role in AKI remains obscure. The aim of our study is to determine the role and mechanism of IGFBP7 in lipopolysaccharide (LPS)-induced HK-2 cells in vitro and on sepsis-induced AKI by cecal ligation and puncture (CLP) in vivo. Here, we identified that IGFBP7 expression was increased in patients with AKI and HK-2 cells with LPS (1, 2, and 5 μg/mL) induction. HK-2 cells with LPS induction showed cell cycle arrest at G1-G0 phases and cell apoptosis and activated ERK1/2 parallel with the changes in the proteins belonging to the ERK1/2 pathway, including Cyclin D1, P21, Bax, and Bcl-2, which were inhibited by the IGFBP7 knockdown. Moreover, IGFBP7 overexpression significantly induced cell cycle arrest at G1-G0 phases and cell apoptosis of HK-2 cells, which were inhibited by PD98509, an ERK1/2 signaling inhibitor. IGFBP7 knockdown effectively alleviated the severity of the renal injury, evidenced by decreases in the urinary levels of creatinine, blood urea nitrogen, and albumin, cell apoptosis, and activation of ERK1/2 signaling in CLP mice. Taken together, our findings indicate that IGFBP7 regulates sepsis-induced AKI through ERK1/2 signaling.
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Drug Design Development and Therapy
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2015
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IF 3.028
Jing Sun,Xueqi Fu,Ye Liu,Yongsen Wang,Bo Huo,Yidi Guo,Xuefeng Gao,Wannan Li,Xin Hu
Background Honokiol is one of the main bioactive constituents of the traditional Chinese herbal drug Magnolia bark (Cortex Magnoliae officinalis , Hou Po). The aim of this study was to probe its anti-type 2 diabetes mellitus effects and the underlying mechanism. Methods Type 2 diabetic mouse model was established by intraperitoneally injecting with streptozotocin. Fasting blood glucose, body weight, and lipid profile were measured. The subcutaneous adipose tissue, skeletal muscle, and liver were isolated as well as homogenized. The phospho-insulin receptor β-subunit (IRβ), IRβ, phospho-AKT, AKT, phospho-ERK1/2, ERK1/2, phosphotyrosine, and actin were examined by Western blot assay. Cell viability or cytotoxicity was analyzed by using MTT method. The inhibitory potencies of honokiol on the protein tyrosine phosphatase 1B (PTP1B) activity were performed in reaction buffer. Molecular docking and dynamic simulation were also analyzed. Results In in vivo studies, oral treatment with 200 mg/kg honokiol for 8 weeks significantly decreases the fasting blood glucose in type 2 diabetes mellitus mice. The phosphorylations of the IRβ and the downstream insulin signaling factors including AKT and ERK1/2 significantly increase in adipose, skeletal muscle, and liver tissue of the honokiol-treated mice. Moreover, honokiol enhanced the insulin-stimulated phosphorylations of IRβ, AKT, and ERK1/2 in a dose-dependent manner in C2C12 myotube cells. Meanwhile, honokiol enhanced insulin-stimulated GLUT4 translocation. Importantly, honokiol exhibited reversible competitive inhibitory activity against PTP1B with good selectivity in vitro and in vivo. Furthermore, using molecular docking and dynamic simulation approaches, we determined the potential binding mode of honokiol to PTP1B at an atomic level. Conclusion These findings indicated the hypoglycemic effects of honokiol and its mechanism that honokiol improved the insulin sensitivity by targeting PTP1B. Therefore, our study may highlight honokiol as a promising insulin sensitizer for the therapy of type 2 diabetes.
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ANALYTICAL CHEMISTRY
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2014
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IF 5.825
Zhi Peng Cai,Andrew Kevin Hagan,Mao Mao Wang,Sabine Lahja Flitsch,Li Liu,Josef Voglmeir
We herein report the use of 1,3-di(2-pyridyl)-1,3-propanedione (DPPD) as a fluorogenic labeling reagent for sugars. Reaction of DPPD with the anomeric carbon affords a fluorescent 2-pyridylfuran (2-PF) moiety that permits the sensitive HPLC-based detection of monosaccharides. 2-PF-labeled monosaccharides can be easily separated and analyzed from mixtures thereof, and the reported protocol compares favorably with established labeling reagents such as 2-aminobenzoic acid (2-AA) and 1-phenyl-3-methyl-5-pyrazolone (PMP), ultimately allowing subfemtomole detection of the galactose-derived product. Furthermore, we demonstrate the application of DPPD in the labeling of monosaccharides in complex biological matrices such as blood and milk samples. We envisage that DPPD will prove to be an excellent choice of labeling reagent in monosaccharide and carbohydrate analysis.
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高影响因子
Journal of Materials Chemistry A
·
2014
·Vol. 2
·期 25
·9559-9565
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IF 0
Fei Xue,Zihui Meng,Fengyan Wang,Qiuhong Wang,Min Xue,Zhibin Xu
A novel 2-D photonic crystal (PC) sensing material for the visual detection of glucose with high selectivity at a physiological ionic strength (150 mM) is developed. A monolayer polystyrene crystalline colloidal array (CCA) is embedded in a phenylboronic acid (PBA) functionalized hydrogel film to diffract light to sensitively report on the hydrogel surface area. This 2-D PC sensor is superior to the previously reported 3-D PC sensors due to its fast preparation and simple detection. The binding of glucose would increase the cross-linking of the hydrogel that could shrink the hydrogel to increase the Debye diffraction ring diameter. At a physiological ionic strength of 150 mM, the 2-D PC sensor exhibits significant sensitivity for glucose across the entire human physiological glucose range. Additionally, the 2-D PC sensor shows high selectivity for glucose rather than other sugars (fructose and galactose). The diffraction color change of the 2-D PC sensor can be observed. For validation, the prepared 2-D PC hydrogel sensor is applied for the sensing of glucose in the artificial tear fluid.
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