ACTA PHARMACOLOGICA SINICA
·
2022
·Vol. 44
·期 3
·499-512
·
IF 7.169
Xu Bing-tian,Li Meng-fan,Chen Ke-chun,Li Xing,Cai Ning-bo,Xu Jiang-ping,Wang Hai-tao
Cannabidiol (CBD) reportedly exerts protective effects against many psychiatric disorders and neurodegenerative diseases, but the mechanisms are poorly understood. In this study, we explored the molecular mechanism of CBD against cerebral ischemia. HT-22 cells or primary cortical neurons were subjected to oxygen-glucose deprivation insult followed by reoxygenation (OGD/R). In both HT-22 cells and primary cortical neurons, CBD pretreatment (0.1, 0.3, 1 μM) dose-dependently attenuated OGD/R-induced cell death and mitochondrial dysfunction, ameliorated OGD/R-induced endoplasmic reticulum (ER) stress, and increased the mitofusin-2 (MFN2) protein level in HT-22 cells and primary cortical neurons. Knockdown of MFN2 abolished the protective effects of CBD. CBD pretreatment also suppressed OGD/R-induced binding of Parkin to MFN2 and subsequent ubiquitination of MFN2. Overexpression of Parkin blocked the effects of CBD in reducing MFN2 ubiquitination and reduced cell viability, whereas overexpressing MFN2 abolished Parkin’s detrimental effects. In vivo experiments were conducted on male rats subjected to middle cerebral artery occlusion (MCAO) insult, and administration of CBD (2.5, 5 mg · kg −1 , i.p.) dose-dependently reduced the infarct volume and ER stress in the brains. Moreover, the level of MFN2 within the ischemic penumbra of rats was increased by CBD treatment, while the binding of Parkin to MFN2 and the ubiquitination of MFN2 was decreased. Finally, short hairpin RNA against MFN2 reversed CBD’s protective effects. Together, these results demonstrate that CBD protects brain neurons against cerebral ischemia by reducing MFN2 degradation via disrupting Parkin’s binding to MFN2, indicating that MFN2 is a potential target for the treatment of cerebral ischemia.
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JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Shuxian Li,Yan Zhao,Fuqiang Hu,Yuechun Jiao,Jianming Zhao,Changyong Li
Ethoxy-substituted benzene has several stable conformations as the single bond in the ethoxy group is capable of internal rotation. Ethoxybenzonitrile may has more conformers due to different orientations of ethoxy with respect to the CN group. In this paper, the potential energy surface (PES) of 2-ethoxybenzonitrile in the ground state was calculated at the level of B3LYP/cc-pvdz, and five different molecular conformers were found on the PES. Only the most stable conformer I (trans) was observed in the supersonic molecular beam experiments. The resonance enhanced multiphoton ionization (REMPI) and mass analyzed threshold ionization (MATI) spectra of 2-ethoxybenzonitrile were measured and Franck-Condon simulations were performed, and the theoretical results are in good agreement with the experimental measurements. The similar molecular structures in S 0 , S 1 , and D 0 states imply the large Franck-Condon factors. The vibronic structures in S 1 and D 0 states were analyzed in detail and the vibronic features were assigned. The MATI spectra follow well the propensity rule Δν = 0, indicating that the molecular structures of the cationic and excited states are similar. Most of the observed vibrations are associated with the ring in-plane distortion. The band origin of the S 1 ←S 0 transition and the accurate adiabatic ionization energy of 2-ethoxybenzonitrile are determined for the first time to be 34 092 ± 2 and 69 796 ± 5 cm −1 , respectively.
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Advanced Optical Materials
·
2023
·Vol. 11
·期 15
·2203140
·
IF 9
Jing Yang,Ziyu Wang,Lu Liu,Le Wang,Zhen Chang,Jianxun Li,Kai Wang,Shengzhong (Frank) Liu
Organic lead halide perovskites are regarded as qualified candidates for the photoactive layers of semitransparent solar cells. The critical aspect of boosting the performance of semitransparent perovskite solar cells (PSCs) to a higher level lies in preparing perovskite films with both excellent photoelectric properties and high transmittance. Herein, it is realized that, through theoretical analysis, perovskite materials with bandgap of 2.00–2.30 eV are suitable for the preparation of semitransparent PSCs that can simultaneously achieve low thermal-energy loss, allow transmission of light for high photopic response, and provide acceptable transmittance for photovoltaic greenhouses. Then the study aims at improving the crystallinity and morphology of wide-bandgap perovskite films by using additive engineering. As such, PSCs with absorber bandgaps of 2.02, 2.16, and 2.32 eV deliver maximum power-conversion efficiencies of 12.08%, 9.17%, and 8.42%, respectively. Then the corresponding semitransparent PSCs are crafted with average transmittance of light detectable by the human eye of 9.41%, 23.26%, and 43.47%, respectively. Furthermore, the crop growth factors are predicted to be 21.25%, 29.48%, and 37.07%, respectively. This work gives full play to bandgap modulation in the fabrication of semitransparent PSCs, which will broaden the application of semitransparent solar cells in the field of building integrated photovoltaics.
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JOURNAL OF CELLULAR BIOCHEMISTRY
·
2018
·Vol. 120
·期 5
·7602-7611
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IF 2.959
Xiaolin Wang,Teng Ma,Xiaojian Wan,Yan Meng,Zhenzhen Zhao,Jinjun Bian,Rui Bao,Xiaoming Deng,Tao Yang
IGFBP7 as an early biomarker has been used to identify patients at risk of developing acute kidney injury (AKI). Nevertheless, its role in AKI remains obscure. The aim of our study is to determine the role and mechanism of IGFBP7 in lipopolysaccharide (LPS)-induced HK-2 cells in vitro and on sepsis-induced AKI by cecal ligation and puncture (CLP) in vivo. Here, we identified that IGFBP7 expression was increased in patients with AKI and HK-2 cells with LPS (1, 2, and 5 μg/mL) induction. HK-2 cells with LPS induction showed cell cycle arrest at G1-G0 phases and cell apoptosis and activated ERK1/2 parallel with the changes in the proteins belonging to the ERK1/2 pathway, including Cyclin D1, P21, Bax, and Bcl-2, which were inhibited by the IGFBP7 knockdown. Moreover, IGFBP7 overexpression significantly induced cell cycle arrest at G1-G0 phases and cell apoptosis of HK-2 cells, which were inhibited by PD98509, an ERK1/2 signaling inhibitor. IGFBP7 knockdown effectively alleviated the severity of the renal injury, evidenced by decreases in the urinary levels of creatinine, blood urea nitrogen, and albumin, cell apoptosis, and activation of ERK1/2 signaling in CLP mice. Taken together, our findings indicate that IGFBP7 regulates sepsis-induced AKI through ERK1/2 signaling.
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POLYMER COMPOSITES
·
2018
·Vol. 40
·期 4
·1595-1601
·
IF 1.943
Ming-En Fei,Tianshun Xie,Wendi Liu,Renhui Qiu
Bamboo fibers (BFs)-reinforced unsaturated polyester (UPE) composites were prepared by hand lay-up compression molding. The UPE resins were modified with 2-acetoacetoxyethyl methacrylate (AAEM) and designed to improve the flexibility and toughness of the BFs–UPE composites. The results indicated that the toughness of the composites significantly increased as the AAEM usage increased. When compared with the untreated UPE composites, the composites obtained from AAEM modified UPE resins (with a 20:80 weight ratio of AAEM to UPE) had 21.7% higher impact strength. Dynamic mechanical analysis showed that incorporation of AAEM significantly increased the damping parameter and decreased glass transition temperature of the composites due to the introduction of flexible segments of AAEM into the UPE resins. Scanning electron microscopy images of the impact-fractured surfaces of the composites revealed that AAEM modification improved the interfacial adhesion between BFs and UPE matrices. The main mechanism for the increase in the toughness of the composites could be due to the increased ductility of the matrices. POLYM. COMPOS., 40:1595–1601, 2019. © 2018 Society of Plastics Engineers
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Yan Li,Tao Li,Long-Qiang Xiao,Yue-Fei Zhang
The polycondensation of 2-pyrazolines is presented. The products are oligomers with the five-membered rings of the 2-pyrazoline as the backbone and the reaction is a polycondensation reaction by decomposition of ROH from the hydrogen atom in the −NH– group and the −OR in the ester group. Metal catalysts, reaction periods, and the addition amounts of the catalyst are investigated to optimize the reaction conditions. The products are down-/up-conversion fluorescence molecules that can significantly enhance cell imaging through the synchronized excitation of visible and near-infrared light and may be widely used in other optical fields.
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Polymer Chemistry
·
2018
·Vol. 9
·期 36
·4556-4565
·
IF 4.927
Ruihong Xie,Yefei Tian,Shaojun Peng,Liren Zhang,Yongzhi Men,Wuli Yang
Stimulus responsive nanogels have great potential in the application of drug delivery. In this work, biodegradable zwitterionic poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) nanogels were fabricated by the reflux precipitation polymerization, which was clean and convenient for the application of drug delivery. The hydrodynamic size of the obtained PMPC nanogels was below 200 nm with a narrow size distribution (polydispersity index below 0.1). Moreover, the nanogels showed excellent protein adsorption resistance and remained stable at high concentrations of salt. Furthermore, the PMPC nanogels exhibited redox-responsive performance, and the degradation rate of the nanogels increased as the concentration of glutathione and the pH value increased. In addition, the nanogels could be fully disrupted into short linear polymers (Mw = 2000 g mol−1) after 24 h degradation. The doxorubicin-loaded nanogels showed low drug leakage under physiological conditions (below 15% in 24 h), while they presented rapid drug release in a reduction environment (above 80% in 24 h). The cell cytotoxicity assays revealed that the blank zwitterionic nanogels were nontoxic to the human lung adenocarcinoma epithelial cells (A549 cells) even at high concentrations (1 mg mL−1), while the DOX-loaded nanogels showed efficient inhibition to the tumor cells.
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CERAMICS INTERNATIONAL
·
2019
·
IF 3.45
Hengrui Qiu,Shengli An,Xuejiao Sun,Huimin Yang,Yongqiang Zhang,Wenxiu He
Due to the unconventional properties of MWCNTs-GONRs (multiwalled carbon nanotubes-graphene oxide nanoribbons), we have tried to use it as a carbon resource for supercapacitors . MWCNTs-GONRs/Ni(OH) 2 electrode was obtained by hydrothermal method . Velvet α-Ni(OH) 2 was prepared above NF (nickel-foam) loaded with MWCNTs-GONRs. This layered design can effectively promote the diffusion of ions and increase the active site for MWCNTs-GONRs/Ni(OH) 2 electrode, thus enhancing the electrochemical performance. The electrode exhibits extraordinary electrochemical performances in electrochemical testing, such as supernal specific capacitance (1713.2 F g −1 ) and prominent working time. In addition, supercapacitors was assembled with MWCNTs-GONRs/Ni(OH) 2 and active carbon as materials. Which represents a prominent energy density (41.23 Wh kg −1 ), high power (6.80 kW kg −1 ) and prominent cycling stability property (95.18%, 3000 times). The electrode prepared in this work provides a clue to enlighten people for energy storage.
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FOOD RESEARCH INTERNATIONAL
·
2017
·
IF 3.086
Yajing Fang,Fuqiang Liang,Kunyuan Liu,Shakeel Qaiser,Siyi Pan,Xiaoyun Xu
Flavonoids are a large group of polyphenols and widely distributed in plant foods. Flavonoids exhibit various biological activities, such as anti-cancer, antioxidant and anti-inflammatory while poor oral bioavailability has been considered as a major hurdle in their use as functional foods. Cellular uptake and efflux of flavonoid implicates their bioavailability. To investigate the cellular uptake and efflux of flavonoids, 27 flavonoids were measured for their cellular uptake in Caco-2 cells with (CUV) and without (CU) the inhibitor of P-glycoprotein (P-gp) verapamil. Then, a quantitative structure-absorption relationship (QSAR) model containing 21 compounds as training set was obtained from their corresponding CU. The model showed good robustness and predictivity with a high cross-validation coefficient (Q 2 ) value of 0.809 and Log of the octanol/water partition coefficient (SlogP) and atomic charge on carbon 5 (Q C5 ) were related to flavonoid uptake. The CUV of some flavonoids were significantly ( p < 0.05 or p < 0.01) higher than their CU, suggesting that specific flavonoids are pumped out by P-gp. The structure-affinity relationship of flavonoids as substrates of P-gp was determined with the presence of 4′-OCH 3 , 3′-OCH 3 and the absence of 3′-OH, 3-OH and 4′-OH favorable for the affinity of flavonoids. These results provide valuable information for screening flavonoids with good absorption and low affinity with transporters.
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PROCEEDINGS OF THE COMBUSTION INSTITUTE
·
2024
·
IF 5.3
Mengqi Wu,Zhongkai Liu,Zhaohan Chu,Xiaoqing Wu,Jiabin Huang,Jiuzhong Yang,Bin Yang,Feng Zhang
A combined experimental and theoretical study was conducted to investigate the weight growth routes from 2-naphthylmethyl radical to tricyclic aromatics. Co-pyrolysis of 2-(chloromethyl)naphthalene with two C 3 H 4 isomers (allene and propyne) were experimentally studied using the flash pyrolysis microreactor employing synchrotron vacuum ultraviolet photoionization mass spectrometry (SVUV-PIMS). Abundant propargyl radicals and naphthylmethyl radicals were detected in the two reaction systems. The similar signal intensities and photoionization efficiency spectrum curves of m/z = 180 (C 14 H 12 ) in both reaction systems are dominantly attributed to the products of the radical-radical reaction of 2-naphthylmethyl and propargyl. The possible candidates of m/z = 180 were assigned via the calculated adiabatic ionization energies . Further investigations on the kinetics of the radical-radical 2-naphthylmethyl + propargyl reaction indicate a severe competition among the adducts and tricyclic aromatics at the typical flame temperatures. At low pressures, the tricyclic C 14 H 11 radicals + H products possess overwhelming advantages, which may contribute to the formation of phenanthrene/anthracene or larger polycyclic aromatic hydrocarbons (PAHs) via the subsequent chain reactions. At atmospheric and higher pressures, most of the products are stabilized to the bicyclic adducts. The present work reveals a potential weight growth route from 2-naphthylmethyl radical to tricyclic aromatics, which should be considered in the model for the growth of PAHs.
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CATALYSIS COMMUNICATIONS
·
2016
·
IF 3.389
Xiaomin Xiang,Bin Zhang,Guoqiang Ding,Jinglei Cui,Hongyan Zheng,Yulei Zhu
The oxidation of furfural in H 2 O 2 and H 2 O 2 –Mg(OH) 2 system were systematically investigated and a rational explanation for the reaction mechanism was proposed. 2-formyloxyfuran, from selective oxidation of HC O group in furfural, was a crucial intermediate. The addition of Mg(OH) 2 suppressed the oxidation of furan ring of furfural and enhanced selectivities of 2(5H)-furanone (44.8%) and succinic acid (38.0%). FT-IR, Gaussian calculation and experimental results indicated that the process of furfural oxidation with H 2 O 2 is homogeneous, and the synergy between dissolved Mg 2 + cations and OH − ions facilitates the HOO − attacking the carbon atom of HC O other than the C C bound of furan ring.
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ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Zhi Peng Cai,Andrew Kevin Hagan,Mao Mao Wang,Sabine Lahja Flitsch,Li Liu,Josef Voglmeir
We herein report the use of 1,3-di(2-pyridyl)-1,3-propanedione (DPPD) as a fluorogenic labeling reagent for sugars. Reaction of DPPD with the anomeric carbon affords a fluorescent 2-pyridylfuran (2-PF) moiety that permits the sensitive HPLC-based detection of monosaccharides. 2-PF-labeled monosaccharides can be easily separated and analyzed from mixtures thereof, and the reported protocol compares favorably with established labeling reagents such as 2-aminobenzoic acid (2-AA) and 1-phenyl-3-methyl-5-pyrazolone (PMP), ultimately allowing subfemtomole detection of the galactose-derived product. Furthermore, we demonstrate the application of DPPD in the labeling of monosaccharides in complex biological matrices such as blood and milk samples. We envisage that DPPD will prove to be an excellent choice of labeling reagent in monosaccharide and carbohydrate analysis.
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