ELECTROPHORESIS
·
2024
·
IF 3
Jiao Long,Yan Gao,Kangjia Sheng,Tao Bao,Sicen Wang
In this study, an efficient and practical method was established to enrich and detect three trace quinolones in fish tissue. Low-cost and environment-friendly cotton threads were coated with PCN-224 using a simple one-pot hydrothermal method. The PCN-224-modified cotton threads (PCN-224@CTs) were characterized to ensure that the crystal was successfully fabricated on the surface of cotton threads. The prepared PCN-224@CTs were wrapped around steel needles to form suspended bars. Key factors of extraction and desorption were optimized, and analytical performance was tested. Suspended bar sorptive extraction coupled with ultra-performance liquid chromatography (UPLC) with a photodiode array detector was used to analyze the quinolones in fish tissue. The detection limits of quinolones were 0.26–1.15 ng/mL, and the recoveries were achieved in the range of 81.31%–115.81%, indicating a simple method with high sensitivity and satisfactory practicability of enriching, separating, and detecting trace quinolones was successfully established.
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ke Zhang,Zhihua Li,Suliman Khan,Mohammad Rezaul Islam Shishir,Huanhuan Zheng,Liying Gao,Jiyong Shi,Xiaowei Huang,Xiaobo Zou
This study aimed to develop a natural colorimetric aerogel based on carboxymethyl cellulose (CMC), sodium alginate (SA), black goji anthocyanin (BGA), and various natural fibers such as cotton fiber (CF), silk fiber (SF), and down fiber (DF) via a freeze-drying approach for monitoring fish freshness. Various colorimetric aerogels, including CMC/SA, CMC/SA/CF, CMC/SA/SF, and CMC/SA/DF were developed and structurally characterized using SEM, FTIR, XRD, TGA, TPA, and BET. CMC/SA aerogel showed higher density (0.054 g/cm 3 ) with no surface pores, while CMC/SA/SF had a large surface area (18.6214 m 2 /g) with uniformly higher porosity (97.113 %) and a lower density (0.024 g/cm 3 ). Moreover, the CMC/SA/SF aerogel exhibited higher mechanical properties and color sensitivity at various pH compared to other aerogels, which was then used as an indicator label for monitoring fish freshness during storage at 4 °C and 25 °C. The label aerogel became blue from purple, when TVB-N and pH values reached 20.03 mg/100 g and 19.50 mg/100 g and pH 6.67 and 6.67 at 4 and 25 °C, respectively, exhibiting fish deterioration. Taken together, the colorimetric aerogel composite CMC/SA/SF shows great potential for application as an intelligent package in monitoring the freshness of fish.
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Haiyan Li,Xiao Zhang,Hongmei Luo,Qilin Huang
In this study, an edible calcium-based metal – organic frameworks (Ca-MOFs) were constructed to remove off-odors. The adsorption behavior and mechanisms of Ca-MOFs for various off-odors were evaluated using kinetic models, isotherms, molecular dynamics, and density functional theory. Compared with zeolite and aluminum oxide, Ca-MOFs exhibited superior adsorption capacity for fish odors, attributed to its plentiful COO- sites and unsaturated Ca 2+ sites. Moreover, Ca-MOFs showed selective adsorption for dimethyl trisulfide (DMTS) with an adsorption rate of 97.3 %, surpassing other odors (42.4 – 84.7 %). Ca-MOFs can completely remove DMTS from surimi and also reduce the levels of aldehydes, alcohols, and heterocyclic substances. Hydrogen bonding and van der Waals forces were identified as the primary driving forces for five off-odors adsorption, with the additional involvement of weak coordination bonds in adsorption of DMTS and trimethylamine (TMA). Overall, this study offers a novel strategy for removing off-odors and broadening the potential applications of MOFs.
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Ye Zi,Jiamin Xu,Shudan Huang,Yulu Zheng,Jiawei Peng,Mengyang Yang,Xichang Wang,Jian Zhong
In this work, octenyl succinic anhydride (OSA) chemical modification with surfactant physical modification was combined to modify bovine bone gelatin (BBG) for emulsion stabilization at pH 6.0 (to simulate acidic food environment). OSA modification decreased the β-sheet percentage and increased β-turn percentage of BBG. Further, the combination of OSA modification with surfactant physical modification had obvious and different effects on the emulsifying properties of BBG. The creaming stability of gelatin/surfactant-stabilized emulsions was dependent on gelatin structure, surfactant type, and preparation pH. The emulsions stabilized by OBBG/Span 80 and OBBG/soybean lecithin (only blurry creaming at day 28) had significantly better creaming stability than other emulsions. These results demonstrated that the combination of OSA modification with surfactant modification could be applied to improve the emulsifying properties of BBG.
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Jiexian Dong,Zhenfeng Li,Yu Wang,Maojun Jin,Yudong Shen,Zhenlin Xu,A.M. Abd El-Aty,Shirley J. Gee,Bruce D. Hammock,Yuanming Sun,Hong Wang
To determine malachite green (MG) and its major metabolite, leucomalachite green (LMG) residual levels in tilapia fish, chemiluminescent enzyme immunoassay (CLEIA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. At first, V H and V L gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against LMG, and then thoroughly by database-assisted sequence analysis. Finally, the productive V H and V L were assembled to an intact scFv sequence and engineered to produce scFv-AP fusion protein. The fusion protein was further identified as a bifunctional reagent for immunoassay, then a sensitive one-step CLEIA against LMG was developed with a half-maximal inhibitory concentration (IC 50 ) and limit of detection (LOD) of 1.3 and 0.04 ng/mL, respectively. The validation results of this novel competitive CLEIA was in line with those obtained by classical HPLC method for determination of total MG in spiked and field incurred samples.
研究领域:
免疫学
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食品科学
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环境科学
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DOI
PubMed
高影响因子
Aquaculture Reports
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2021
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IF 3.216
Shiwei Xie,Dan Wei,Lixia Tian,Yongjian Liu
Bile acid metabolism in aquatic animals usually is suppressed when fish meal (FM) is replaced by plant proteins. An eight weeks feeding experiment was conducted to evaluate the effects of dietary chenodeoxycholic acid (CDCA) supplementation on growth, immune response and intestinal health of Penaeus monodon fed a low FM diet. Three diets were formulated: high FM (25 %) diet (HF), low FM (15 %) diet (LF), and low FM diet supplemented with 0.1 % CDCA (LFC). Shrimp fed Diet LF showed lower weight gain and feed efficiency than those fed Diet HF. Low dietary FM up-regulated the mRNA level of xbp1 , and down-regulated the mRNA levels of sod , iap and NF-κB signaling pathway-related genes ( traf6 , tube ). Dietary supplementation of CDCA up-regulated the expression of relish and anti-oxidative-related genes ( sod and cat ) in the hepatopancreas and midgut. Low dietary FM impaired the intestinal fold and induced the endoplasmic reticulum swollen of intestinal epithelial cell, dietary supplementation of CDCA alleviated this problem. The intestinal microbiota diversity was lower in shrimp fed Diet LF than those fed HF. Dietary supplementation of CDCA in a low FM diet increased the intestinal microbiota diversity by decreasing the relative abundance of dominant phylum Proteobacteria. Low dietary FM increased the relative abundance of a dominant pathogenic bacterium ( Vibrio ), which was decreased by the supplementation of CDCA. These results indicated that dietary supplementation of CDCA in a low FM diet improved the growth performance, immune response and intestinal health of shrimp.
研究领域:
水产养殖学
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营养学
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分子生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 8
·5366-5375
·
IF 3.612
Jing Lv,Xinping Lin,Wenqing Wang,Wenhuan Xu,Caichan Li,Chaofan Ji,Huipeng Liang,Shengjie Li,Sufang Zhang,Beiwei Zhu
Summary Suanzhayu is a traditional Chinese fermented fish that often faces a long fermentation time (about 1 month) and unstable quality. This study evaluated the combined effects of papain and Lactiplantibacillus plantarum 1-24-LJ, a selected starter culture, on the quality of Suanzhayu . The addition of L. plantarum and/or papain increased the content of alcohols, esters, ketones and umami amino acids (UAAs), but decreased the content of bitter amino acids (BAAs). Besides, Lactobacillus might play a role in reducing harmful bacteria and BAAs content and increasing UAAs content, while Lactococcus and Weissella contributed to the characteristic flavour of Suanzhayu . Overall, no significant improvement in sensory quality was observed with the addition of papain alone, while better quality and faster fermentation processes were obtained with the addition of L. plantarum 1-24-LJ alone or in combination with papain. The results contribute to quality control in Suanzhayu production and the selection of starter cultures.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
Animal Nutrition
·
2026
·
IF 8.3
Anran Wang,Jie Xu,Haoyue Guo,Dongxu Luo,Zhen Zhang,Chao Ran,Shuyan Miao,Zhigang Zhou
Fish oil is an essential lipid source in aquafeed due to its high polyunsaturated fatty acid content. However, its susceptibility to oxidation can harm aquatic animal health and growth. This study investigated the effects of oxidized fish oil (OFO) diet on hepatopancreas health of Macrobrachium rosenbergii , and the mechanism by which Cetobacterium somerae protects against oxidative damage induced by OFO diet. A total of 240 juvenile prawns (3.36 ± 0.02 g) were randomly allocated to three dietary treatments with four replicates of 20 prawns each for 8 weeks: Control (control diet with fresh fish oil diet), OFO (OFO diet), and OFO + Cet (OFO diet supplemented with C. somerae at 1.52 × 10 7 CFU/g). Compared with the OFO group, C. somerae intervention significantly reduced the levels of lipid peroxidation-related markers, including malondialdehyde (MDA), 5-hydroperoxyeicosatetraenoic acid (5-HETE), leukotriene B4 (LTB4), prostaglandins (PGs), and thromboxanes (TXs), while increasing hepatopancreatic antioxidant indicators, including total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GSH-Px) ( P < 0.05). Moreover, it also reversed the OFO diet-induced alterations in the relative mRNA expression levels of ferroptosis-related genes ( acsl4 , tfr1 , gpx4 , and slc7a11 ) ( P < 0.05), potentially via the adenosine monophosphate (AMP)-activated protein kinase (AMPK)-sirtuin1 (SIRT1)-peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) signaling pathway. Serum acetic acid, a C. somerae metabolite, exhibited the most pronounced alteration in the C. somerae -treated group ( P = 0.004). Furthermore, 160 juvenile prawns (2.62 ± 0.12 g) were randomly divided into two groups with four replicates of 20 prawns in each for 4 weeks: OFO diet, OFO + 0.15% sodium acetate (0.15% NaAc). Supplementation with 0.15% NaAc in the OFO diet also effectively decreased the peroxides (MDA, 5-HETE, LTB4, and TXs) and reversed the OFO diet-induced alterations in the relative mRNA expression levels of ferroptosis-related genes ( acsl4 , tfr1 , gpx4 , and slc7a11 ) compared with OFO group ( P < 0.05), suggesting a contributory role of acetate in the functionality of C. somerae . Meanwhile, the elevated AMP/adenosine triphosphate (ATP) ratio and upregulated relative mRNA expression levels of acss1/2 , ampk, sirt1 , and pgc-1α ( P < 0.05) indicated that C. somerae- derived acetate activates AMP production, thereby activating the AMPK-SIRT1-PGC-1α signaling pathway . Overall, C. somerae protects against OFO diet-induced hepatopancreatic injury by mitigating ferroptosis via acetate-mediated activation of the AMPK-SIRT1-PGC-1α signaling pathway.
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wenyu Guo,Yuanyuan Zhang,Yunxuan Wang,Zhijun Hu,Peixuan Xu,Jingying Cai,Peirong Yu,Shiya Chen,Lei Zhang,Benmei Wei,Juntao Zhang,Sheng Li,Haibo Wang,Lingyun Chen
Peptides, which are hydrolysis products of proteins, are commonly utilized as nutritional supplements in food due to their high bio-safety profile. In this study, we present a novel water-soluble fluorescent probe derived from fish collagen peptides, termed Sali-Pep. This probe exhibits light green fluorescence, characterized by both aggregate-induced-emission (AIE) and excited state intramolecular proton transfer (ESIPT) mechanisms, with emission peaks at 480 nm and 522 nm. Furthermore, Sali-Pep demonstrates a high sensitivity towards Cu 2+ ions via a “turn-off” fluorescence mechanism, and towards Zn 2+ ions through a “turn-on” fluorescence mechanism, both in a dose-dependent manner. The detection limits of Sali-Pep were 1.49 × 10 −7 M for Cu 2+ and 4.23 × 10 −7 M for Zn 2+ , respectively. In real environmental and biological samples, the recoveries of Cu 2+ and Zn 2+ ions ranged from 91.14 % to 108.61 %. These findings indicate that Sali-Pep is a promising fluorescent probe for the detection of Cu 2+ and Zn 2+ ions, combining both high biosafety and low environmental risk. Additionally, this study also show that collagen-derived peptides have the potential to serve as AIE sensing platforms for metal ion detection and fluorescent labeling.
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DOI
高影响因子
Sustainable Chemistry and Pharmacy
·
2020
·
IF 3.294
Qing Feng Yao,Dong Sheng Zhou,Jin Hua Yang,Wei Tao Huang
Fluorescent nanomaterials have important applications in environmental monitoring and biomedicine and imaging. However, their preparation often is cumbersome, expensive, and extremely small-scale, requires harsh conditions, which limit their wide and practical applications. Herein, by directly reusing waste fish scales with intrinsic fluorescent property as precursors, we developed a facile, large-scale, inexpensive, and green method for extraction of fluorescent carbon nanoparticles for sensing of Fe 3+ . Our preparation approach only requires soaking fish scales in water without harsh conditions and complicated operations, only based on physical exfoliation, not limited by containers. Fish-scale-derived fluorescent carbon nanoparticles with bright blue fluorescence and a quantum yield of 15.6% are irregular spheres with a diameter of more than 60 nm, contained smaller nanodots with a diameter of about 4.2 nm, which has obvious lattice fringes of 0.23 nm. Elemental and functional group analysis confirmed that fluorescent carbon nanoparticles were mainly composed of C, O, and N, had hydroxyl, amine, and carbonyl groups, and similar in composition to collagens and hydroxyapatites . Fluorescent carbon nanoparticles can be used for fluorescent quenching-based detection of Fe 3+ with a linear range of 0–6.25 μM and detection limit of 0.144 μM, and have good recoveries for Fe 3+ in actual water samples and human serum with low relative standard deviations. This study not only adds a new dimension to provide a large-scale, simple, green, and promising method for reuse of waste fish scales, but also offers a new idea for the large-scale preparation and application of fluorescent carbon nanomaterials.
研究领域:
材料科学
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环境科学
·
生物医学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Xin Song,Rongyu Wang,Xiao Wang,Haoyue Han,Zhaoyu Qiao,Xiaowei Sun,Wenhua Ji
Due to the environmental persistence and various health problems associated with per- and polyfluoroalkyl substances (PFASs), they have come under increased public scrutiny. However, the efficient extraction of PFASs from complex media remains challenging. Herein, an olefin-linked covalent organic framework (COF-CN) has been prepared via a Knoevenagel condensation reaction , followed by reduction using LiAlH 4 to form an amine-functionalized COF (COF-NH 2 ). The characterization results demonstrated that the crystal structure was maintained during the post-modification step. Isothermal and kinetic adsorption studies showed the higher affinity of COF-NH 2 toward PFASs. Based on density functional theory , the adsorption mechanism of the stable six-member-ring structure formed between COF-NH 2 and PFASs via hydrogen bonding was tentatively revealed. After optimizing the solid-phase microextraction parameters, legacy and emerging PFASs were efficiently extracted from fish using the COF-NH 2 coating, followed by detection using ultra-performance liquid chromatography-tandem mass spectrometry . The method exhibited ideal linearity, low limits of quantification, excellent precision, and high relative recoveries. Finally, the bioconcentration kinetics for goldfish was studied, which can provide a feasible platform for investigating the accumulate ion and toxicity of PFASs.
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Zi-Yuan Zhao,Lei Qin,Meng Dong,Yu-Ying Zhang,Xu-Hui Huang,Ming Du,Da-Yong Zhou,Bei-Wei Zhu
Intake of endocrine-disrupting chemicals (EDCs) by humans could disturb the metabolism of hormones, induce cancer, and damage the liver and other organs. Phthalate acid esters (PAEs) and alkylphenols (APs) are important EDCs and environmental contaminants. With the increasing use of plastics and nonionic surfactants worldwide, PAEs and APs have entered environmental water and accumulated in edible fish, which are finally consumed by humans. In this study, a coated direct inlet probe (CDIP) based on an atmospheric solid analysis probe, which can rapidly and simultaneously extract both PAEs and APs in fish, was developed. Twelve PAEs and APs were quantified by using a stable-isotope-labeled internal standard. Standard curves of the PAEs and APs having correlation coefficients of R2 ≥ 0.9837 were obtained. The limit of detection of the PAEs and APs was distributed from 0.01 to 40 ng g–1. The relative recovery of the method was 78–120% between low, medium, and high spiked levels. Combined with principal component analysis, PAE- and AP-contaminated Carassius auratus from different habitats could be identified. Multiple sample analysis mode allowed the extraction of up to 12 samples at once, and the total analysis time (including sample pretreatment, extraction, and analysis time) was less than 10 min per sample, which indicates that CDIP is useful for rapid quantitative analysis.
研究领域:
环境科学
·
分析化学
·
食品安全
·
毒理学
·
质谱分析
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DOI
高影响因子
危险品化学品经营许可证（带存储）