JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY
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2018
·
IF 1.418
Hui Chen,Wei Song,Kun-Kun Sun,He-Wei Du,Shu-Dong Wei
This study aimed to investigate the structural features, antioxidant activity, tyrosinase inhibitory effect, and mechanism of proanthocyanidins from leaf and fruit of Leucaena leucocephala. MALDI-TOF-MS, thiolysis coupled with HPLC-ESI-MS, and 13C-NMR confirmed that these proanthocyanidins were complex mixtures of propelargonidins, procyanidins, and prodelphinidins. (Epi)catechin, (epi)gallocatechin, (epi)catechin gallate, and (epi)gallocatechin gallate were the main constitutive units. The findings obtained from enzyme analysis revealed that the proanthocyanidins had inhibitory effects on both monophenolase and diphenolase of tyrosinase. The IC50 values of leaf and fruit proanthocyanidins were 73.5 ± 2.7 and 52.3 ± 3.5 µg/mL for monophenolase activity, and 27.2 ± 1.4 and 16.1 ± 1.1 µg/mL for diphenolase activity, respectively. The inhibition of diphenolase by proanthocyanidins were proved to be reversible and mixed type. In addition, it was also found from various antioxidant methods that the proanthocyanidins in leaf and fruit possessed potent DPPH radical scavenging activity with IC50 values of 103.4 ± 0.8 and 87.8 ± 1.1 µg/mL, ABTS radical scavenging activity with IC50 values of 72.9 ± 0.4 and 65.7 ± 0.9 µg/mL, and ferric reducing antioxidant power with FRAP values of 3.74 ± 0.03 and 4.02 ± 0.15 mmol AAE/g, respectively. The results obtained suggested that the proanthocyanidins from leaf and fruit of L. leucocephala might have the potential to be exploited as natural tyrosinase inhibitors and antioxidants.
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Chemistry Central Journal
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2014
·Vol. 8
·期 1
·1-7
·
IF 1.663
Luo Hui,Cai Yongqiang,Peng Zhijun,Liu Tao,Yang Shengjie
Background Hylocereus polyrhizus and Hylocereus undatus are two varieties of the commonly called pitaya fruits, and pitaya fruits have gained popularity in many countries all over the world. However, studies on chemical composition and the nutritional quality of pitaya flesh peel are limited.Results Extracts of pitaya (H. polyrhizus and H. undatus) peel were extracted by supercritical carbon dioxide extraction, and analyzed by gas chromatography–mass spectrometry analysis. Their cytotoxic and antioxidant activities were investigated. The main components of H. polyrhizus extract were β-amyrin (15.87%), α-amyrin (13.90%), octacosane (12.2%), γ-sitosterol (9.35%), octadecane (6.27%), 1-tetracosanol (5.19%), stigmast-4-en-3-one (4.65%), and campesterol (4.16%), whereas H. undatus were β-amyrin (23.39%), γ-sitosterol (19.32%), and octadecane (9.25%), heptacosane (5.52%), campesterol (5.27%), nonacosane (5.02%), and trichloroacetic acid, hexadecyl ester (5.21%). Both of the two extracts possessed good cytotoxic activities against PC3, Bcap-37, and MGC-803 cells (IC50 values ranging from 0.61 to 0.73 mg/mL), and the activities of their main components were also studied. Furthermore, these extracts also presented some radical scavenging activities, with IC50 values of 0.83 and 0.91 mg/mL, respectively.Conclusion This paper provides evidence for studying the chemical composition of supercritical carbon dioxide extracts of pitaya peel and their biological activity.
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MOLECULES
·
2014
·Vol. 19
·期 4
·4510-4523
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IF 2.095
Feng Chen,Hai-Long Li,Yin-Feng Tan,Wei-Wei Guan,Jun-Qing Zhang,Yong-Hui Li,Yuan-Sheng Zhao,Zhen-Miao Qin
Plant secondary metabolites are known to not only play a key role in the adaptation of plants to their environment, but also represent an important source of active pharmaceuticals.Alpinia oxyphyllacapsular fruits, made up of seeds and pericarps, are commonly used in traditional East Asian medicines. In clinical utilization of these capsular fruits, inconsistent processing approaches (i.e., hulling pericarps or not) are employed, with the potential of leading to differential pharmacological effects. Therefore, an important question arises whether the content levels of pharmacologically active chemicals between the seeds and pericarps ofA. oxyphyllaare comparable. Nine secondary metabolites present inA. oxyphyllacapsular fruits, including flavonoids (e.g., tectochrysin, izalpinin, chrysin, apigenin-4',7-dimethylether and kaempferide), diarylheptanoids (e.g., yakuchinone A and B and oxyphyllacinol) and sesquiterpenes (e.g., nootkatone), were regarded as representative constituents with putative pharmacological activities. This work aimed to investigate the abundance of the nine constituents in the seeds and pericarps ofA. oxyphylla. Thirteen batches ofA. oxyphyllacapsular fruits were gathered from different production regions. Accordingly, an ultra-fast high performance liquid chromatography/quadrupole tandem mass spectrometry (UFLC-MS/MS) method was developed and validated. We found that: (1) the nine secondary metabolites were differentially concentrated in seeds and fruit capsules; (2) nootkatone is predominantly distributed in the seeds; in contrast, the flavonoids and diarylheptanoids are mainly deposited in the capsules; and (3) the content levels of the nine secondary metabolites occurring in the capsules varied greatly among different production regions, although the nootkatone levels in the seeds were comparable among production regions. These results are helpful to evaluating and elucidating pharmacological activities ofA. oxyphyllacapsular fruits. Additionally, it may be of interest to elucidate the mechanisms involved in the distinct accumulation profiles of these secondary metabolites between seeds and pericarps.Keywords:secondary metabolites;A. oxyphyllacapsular fruits;diarylheptanoids;flavonoids;nootkatone;production region;UFLC-MS/MS
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FOOD CHEMISTRY
·
2013
·
IF 3.334
Miaomiao Tian,Rongmin Cheng,Juanjuan Ye,Xiaoting Liu,Qiong Jia
A new ionic liquid (IL)-calixarene coated solid-phase microextraction (SPME) fibre has been synthesized on the surface of quartz fibre by the sol–gel method. The coated fibre has been coupled with gas chromatography–flame ionisation detector (GC–FID) for the determination of triazines in fruit and vegetable samples. The operation parameters including sample volume, extraction time, extraction temperature, desorption time, and sample pH have been investigated and optimised. Under the optimum conditions, the limits of detection of atrazine, simazine, ametryn, and cyanazine based on three times of standard deviations of blank by seven replications are 3.3, 4.4, 8.8, and 13.0 μg kg −1 , respectively. The intra-day and inter-day relative standard deviations are less than 7.2% and 9.9%. The proposed method has been successfully applied to the determination of the four triazines in fruit and vegetable samples and the accuracy is assessed through recovery experiments.
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Analytical Methods
·
2016
·Vol. 8
·期 17
·3528-3535
·
IF 1.915
Xingli Liu,Tao Feng,Chenhuan Wang,Lin Hao,Chun Wang,Qiuhua Wu,Zhi Wang
In this work, a nanoporous carbon/iron composite material designated as MIL-53-C was fabricated by one-step carbonization of an Fe-based metal–organic framework material MIL-53. The obtained MIL-53-C showed a high Brunauer–Emmett–Teller surface area (249.33 m2 g−1), large pore volume (0.57 cm3 g−1) and an excellent magnetic property (127.95 emu g−1). It was used as a magnetic solid-phase extraction adsorbent for the enrichment of some endocrine disrupting compounds from fruit juice and milk samples followed by high-performance liquid chromatographic analysis. Under optimum conditions, a good linearity for all analytes over the studied concentration range was achieved with a correlation coefficient higher than 0.9995. The limits of detection, based on S/N = 3, were 0.05–0.10 ng mL−1 for fruit juice and 0.10–0.20 ng mL−1 for milk samples. The recoveries of spiked samples ranged from 92.2% to 108.3%. These results indicated that the MIL-53-C exhibited an excellent adsorption capability for trace levels of EDCs, and it may also be a promising adsorbent for extraction and enrichment of other organic pollutants.
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MOLECULES
·
2016
·Vol. 21
·期 9
·1148
·
IF 2.465
Shuxia Yan,Xianan Zhang,Xin Wen,Qiang Lv,Changjie Xu,Chongde Sun,Xian Li
Chinese bayberry (Morella rubraSieb. et Zucc.) fruit have a diverse flavonoid composition responsible for the various medicinal activities, including anti-diabetes. In the present study, efficient simultaneous purification of four flavonoid glycosides, i.e., cyanidin-3-O-glucoside (1), myricetin-3-O-rhamnoside (2), quercetin-3-O-galactoside (3), quercetin-3-O-rhamnoside (4), from Chinese bayberry pulp was established by the combination of solid phase extract (SPE) by C18 Sep-Pak®cartridge column chromatography and semi-preparative HPLC (Prep-HPLC), which was followed by HPLC and LC-MS identification. The purified flavonoid glycosides, as well as different fractions of fruit extracts of six bayberry cultivars, were investigated for α-glucosidase inhibitory activities. The flavonol extracts (50% methanol elution fraction) of six cultivars showed strong α-glucosidase inhibitory activities (IC50= 15.4–69.5 μg/mL), which were higher than that of positive control acarbose (IC50= 383.2 μg/mL). Four purified compounds1–4exerted α-glucosidase inhibitory activities, with IC50values of 1444.3 μg/mL, 418.8 μg/mL, 556.4 μg/mL, and 491.8 μg/mL, respectively. Such results may provide important evidence for the potential anti-diabetic activity of different cultivars of Chinese bayberry fruit and the possible bioactive compounds involved.Keywords:Morella rubrafruit;α-glucosidase inhibitory activity;flavonoid glycosides;purification
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FOOD CHEMISTRY
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2017
·
IF 4.529
Dezhi Yang,Xiaolan Li,Dongling Meng,Meng Wang,Yaling Yang
A magnesium–aluminum layered double hydroxides coated on magnetic nanoparticles (Fe 3 O 4 @MgAl-LDHs NPs) combined with the supramolecular solvents (SUPRASs) as the magnetic supramolecular fluids were successfully applied to the extraction and determination of four phenolic compounds (Bisphenol-A (BPA), bisphenol-AF (BPAF), tetrabromobisphenol-A (TBBPA) and 4-tert-octylphenol (4-TOP)). Magnetic supramolecular fluids (ferrofluids) composites were prepared through adding the mixture of n -octanol and tetrahydrofuran in distilled water as the supramolecular solvents into the solution containing magnetic Fe 3 O 4 @MgAl-LDHs NPs. Under the optimized conditions, the calibration curve was obtained in range of 5–1000 μg L −1 . The limits of detection (S/N = 3) for BPA, BPAF, TBBPA and 4-TOP, were obtained for 0.54, 0.48, 0.37 and 0.63 μg L −1 , respectively. The intra-day and inter-day precision (RSDs %) for bisphenols (BPs) and 4-TOP at 100 and 300 μg L −1 were in the range of 4.0–7.8. The enrichment factors were between 16 and 24.
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JOURNAL OF FOOD BIOCHEMISTRY
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2024
·Vol. 2024
·期 1
·3900785
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IF 3.5
Wei Ren,Jian Xu,Yongping Zhang,Yanyan Miao,Yao Liu
The aim of this study is to investigate efficient, cost-effective, and high-quality drying technologies for Rosa roxburghii Tratt. Five drying methods—hot air drying (HAD), microwave drying (MD), vacuum drying (VD), microwave vacuum drying (MVD), and freeze vacuum drying (FVD), were employed to dry Rosa roxburghii Tratt. The study focused on evaluating drying characteristics, colors, texture and structure, and microstructure, as well as the content of active ingredients and antioxidant activity of Rosa roxburghii Tratt. The results of drying kinetics indicated that the Wang and Singh model was the most suitable for FVD, while the Page model was optimal for the other four drying methods. Rosa roxburghii Tratt subjected to HAD exhibited the highest energy consumption per unit but the lowest ΔE and the highest TFC. The microstructure of Rosa roxburghii Tratt under different drying methods was analyzed. Rosa roxburghii Tratt dried using MD experienced serious cell rupture, resulting in a large loss of active ingredients and the worst antioxidant ability, but it had the fastest drying rate. On the other hand, Rosa roxburghii Tratt dried under VD had the microstructure with the least pore space, leading to slower drying speed and the highest total polyphenol content. Rosa roxburghii Tratt dried using MVD had the least hardness and chewiness, with slightly better Δ E and active ingredient content compared to MD. FVD Rosa roxburghii Tratt took the longest time to dry, with the greatest Δ E difference, hardness, and chewiness, but it had the highest Vitamin C content and the most powerful antioxidant ability. Principal component analysis (PCA)–entropy weighting method was used to provide comprehensive scores for the different drying methods, with FVD, HAD, VD, MVD, and MD were 0.8107, 0.7037, 0.4499, 0.2746, and 0.0586 respectively. Through comprehensive analysis, we demonstrated that the quality of Rosa roxburghii Tratt obtained via FVD and HAD is satisfactory. Additionally, HAD is characterized by its economic efficiency and convenience, providing valuable insights for the industrial production of Rosa roxburghii Tratt drying.
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FOOD RESEARCH INTERNATIONAL
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2025
·
IF 8
Nisar Hussain,Yuzhu Li,Ning Li,Abid Hussain,Manzoor Hussain,Mengke Su,Honglin Liu
Surface-enhanced Raman scattering (SERS) is an emerging swift and nondestructive detecting method, offering a unique structural signature of analytes down to ultrasensitive levels. Herein, we present a novel SERS-based core-shell nanostructure with optimized silver shell thicknesses, capped with 2-mercaptoethylamine (2-MCE), to simultaneously detect multiple agrochemical residues in fruit samples. Compared to unmodified Au@AgNPs, the modified Au@Ag@2-MCENPs integrated with SERS exhibited improved sensitivity and stability, exhibiting a strong relationship between the SERS intensities and pesticide concentrations in the fruit samples, with LODs of 0.006 and 0.008 ppm for thiacloprid and 0.007 and 0.009 ppm for oxamyl in pear and apple fruit samples, respectively. Moreover, the method showed satisfactory recoveries ranging from 80 % to 106.5 % for thiacloprid and 81.8 % to 110.2 % for oxamyl in both fruit samples. Furthermore, this method presented rapid and simultaneous detection capabilities, significantly reducing analysis time compared to traditional methods, making it suitable for detecting other toxic chemical residues in agricultural products.
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Food Bioscience
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2024
·
IF 4.8
Xiaomei Ye,Shiru Mo,Mingyue Shen,Qiang Yu,Yi Chen,Chengyuan Wang,Xianxiang Chen,Jianhua Xie
Allergic asthma accounts for more than 50 percent of adult asthma in life. Glucocorticoids and leukotriene receptor antagonists are commonly used to treat asthma, but these drugs not only have high side effects but also cannot cure the disease. In contrast, natural active ingredients are relatively safe to the organism and show great potential for improving the organism. Polysaccharides, as the main active ingredient in aqueous extract, may be equally beneficial for biological activity. The aim of this study is to investigate the alleviating effect and mechanism of action of Ficus pumila fruit polysaccharide (FPP) and aqueous extract of Ficus pumila fruit (FPW) on mice with allergic asthma. The results showed that FPP and FPW could regulate the secretion of the cytokines IL-4, IL-5, IL-13, and IFN-γ. FPP and FPW also reduced the inflammation, mucus secretion, and collagen deposition in mouse lung tissue, and down-regulated the expression of matrix metallopeptidase-9 (MMP-9), tissue inhibitor of metalloproteinases-1 (TIMP-1), and α-smooth muscle actin (α-SMA) proteins. In addition, FPP and FPW modulated the intestinal microbiota by augmenting the alpha diversity and adjusting the taxonomic composition at the phylum and family levels, thereby ensured the sustenance of regular physiological processes in the body. This study demonstrated that FPP and FPW may attenuate asthma by inducing beneficial microbiota in the gut-lung axis and provide an effective basis for the future clinical treatment of allergic asthma with Ficus pumila and the development and utilization of new anti-asthma drugs.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Ting-tiao Pan,Da-Wen Sun,Hongbin Pu,Qingyi Wei
A simple method based on surface-enhanced Raman scattering (SERS) was developed for the rapid determination of alternariol (AOH) in pear fruits using an easily prepared silver-nanoparticle (AgNP) substrate. The AgNP substrate was modified by pyridine to circumvent the weak affinity of the AOH molecules to the silver surface and to improve the sensitivity of detection. Quantitative analysis was performed in AOH solutions at concentrations ranging from 3.16 to 316.0 μg/L, and the limit of detection was 1.30 μg/L. The novel method was also applied to the detection of AOH residues in pear fruits purchased from the market and in pear fruits that were artificially inoculated with Alternaria alternata. AOH was not found in any of the fresh fruit, whereas it resided in the rotten and inoculated fruits. Finally, the SERS method was cross validated against HPLC. It was revealed that the SERS method has great potential utility in the rapid detection of AOH in pear fruits and other agricultural products.
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Horticultural Plant Journal
·
2025
·
IF 6.2
Yukuo Li,Zhe Song,Xu Zhan,Miaomiao Lin,Xiaohan Li,Ran Wang,Leiming Sun,Hong Gu,Feng Wei,Jinbao Fang,Xiujuan Qi
In kiwifruit ( Actinidia arguta ), fruit coloration is typically accompanied by ripening; however, the intrinsic connection between these two processes remains unclear. In this study, we found that ethylene and auxin accelerated and suppressed fruit coloration and ripening, respectively, in A. arguta . ETHYLENE INSENSITIVE 3-LIKE 2 (AaEIL2) and ETHYLENE RESPONSIVE FACTOR (AaERF059) were mined and identified using yeast two-hybrid library screening and transcriptome analysis. AaEIL2, specifically induced to high expression by ethylene, was confirmed as a transcription factor that positively regulates coloration and ripening by targeting AaLDOX (leucoanthocyanidin dioxygenase involved in anthocyanin biosynthesis) and AaPG18 (polygalacturonase involved in cell wall degradation) based on its subcellular localization in Arabidopsis protoplasts, stable genetic transformation in transgenic tomato, and yeast one-hybrid and luciferase activity assays. AaERF059 also responds to ethylene and regulates ethylene-/auxin-mediated fruit coloration and ripening by targeting the downstream genes AaACS2 (ACC synthase, which is involved in ethylene biosynthesis) and AaGH3 (Gretchen-Hagen 3, which is involved in the auxin pathway). Overall, AaEIL2 and AaERF059 regulate ethylene-and auxin-mediated fruit coloration and ripening by maintaining a dynamic balance in a positive and negative regulatory manner. Our results not only identified key genes but also established an intrinsic connection between fruit coloration and ripening in A. arguta .
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危险品化学品经营许可证（带存储）