METABOLIC ENGINEERING
·
2024
·
IF 6.8
Qingchen Li,Chenxi Li,Jie Zhong,Yukun Wang,Qinghua Yang,Bingmei Wang,Wenjin He,Jianzhong Huang,Shengyuan Lin,Feng Qi
N-methylserotonin (NMS) is a valuable indole alkaloid with therapeutic potential for psychiatric and neurological disorders, and it is used in health foods, cosmetics, and weight loss supplements. However, environmental challenges and low reaction efficiencies significantly hinder cost-effective, large-scale production of NMS in plants or through chemical synthesis. Herein, we have successfully engineered Escherichia coli strains to enhance NMS production from L-tryptophan using whole-cell catalysis. We developed multiple biosynthesis pathways incorporating modules for serotonin (5-hydroxytryptamine, 5-HT), tetrahydromonapterin (MH₄), and S-adenosylmethionine (SAM) synthesis. To enhance MH₄ availability, we employed a high-activity Bacillus subtilis FolE and minimized carbon flux loss through targeted gene knockouts in competitive metabolic pathways, improving 5-HT production. Additionally, we constructed a comprehensive SAM biosynthesis module to facilitate transmethylation by a selected N-methyltransferase fused with ProS2. These engineered modules were coexpressed in two plasmids within the optimized strain NMS-19, producing 128.6 mg/L of NMS in a 5-L bioreactor using fed-batch cultivation—a 92-fold increase over the original strain. This study introduces a viable strategy for NMS production and provides insights into the biosynthesis of SAM-dependent methylated tryptamine derivatives.
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高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Xuan Tang,Haidong Xu,Yuqing Shi,Mingwei Wu,Hongru Tian,Jie Liang
The porous antimicrobial starch particles containing N-Halamine functional groups (PST-MBA-Cl particles) were synthesized by a crosslinking polymerization between starch (ST) and N, N' -methylenebisacrylamide (MBA), and then a chlorination of amide groups of MBA . The synthetic process used only water as the solvent and was environmentally friendly. The results showed that under the optimal preparation conditions, the as-synthesized PST-MBA-Cl particles could have a Cl + % of 8.60 %. Antimicrobial tests showed that PST-MBA-Cl particles had very powerful antimicrobial efficacy against both Staphylococcus aureus and Escherichia coli and could completely kill Staphylococcus aureus with a concentration of 2.1 × 10 6 CFU/mL and Escherichia coli with a concentration of 5.6 × 10 6 CFU/mL within a contact time of one minute. Furthermore, the N-Halamine functional groups of PST-MBA-Cl particles also showed excellent stability under storage and reproducibility. Therefore, the as-synthesized PST-MBA-Cl particles will have potential applications in water disinfection.
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Advanced Powder Materials
·
2023
·
IF 0
Zongge Li,Chenwei Wang,Anuj Kumar,Hongrui Jia,Yin Jia,Huifang Li,Lu Bai,Guoxin Zhang,Xiaoming Sun
Despite the fact that low-dimensional carbons (LDCs, 1D/2D) materials are very interesting due to their intriguing electrical properties, we still attempt to enrich them by high N-content in order to enjoy their electro-applications. We here report a template-free synthesis of 1D/2D LDC with high N content (>40 at%) and tunable aspect ratios from molecular formamide (FA). The 1D/2D LDC is in polyaminoimidazole as confirmed by pair distribution function analysis, and 1D growth mode can be altered to 2D by simply adding a 2D-guiding molecule of melamine. Electrochemical properties of the LDC can be finely tuned by adjusting the solvothermal temperature and melamine dosage. It is revealed that the optimal 2D LDC delivers superior O 2 -to-H 2 O 2 yield (687.2 mmol·g −1 ⋅h −1 ) and Faradic efficiency (87.5%). Considering the heavy N content and high adjustability of aspect ratio, the FA-derived LDCs potentially open new synthesis routes for structural carbon materials for broad electrochemical applications.
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TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY
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2016
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IF 0.634
Xirui Lv,Yue Zhou,Anguo Zhang,Lihong Zhou,Qingle Zeng
A straightforward and environment-friendly protocol for the synthesis of valuable chiral N-(tert-butylsulfinyl)imines has been developed. Different from traditional process with benzaldehydes as substrates, arylmethyl alcohols, benzylthiol, dibenzyl ether, thioether, and disulfide are used as alternative substrates to react with tert-butanesulfinamide in the presence of KOtBu under air for the synthesis of chiral N-(tert-butylsulfinyl)imines. This is a transition metal-free, mild, cost-effective, and simple process.
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CHINESE JOURNAL OF CHEMICAL PHYSICS
·
2022
·Vol. 35
·期 5
·823-834
·
IF 1.09
Wu Xiao-ran,Xue Xiang,Wang Jing-hang,Liu He-wen
Smart functional microgels hold great potential in a variety of applications, especially in drug transportation. However, current drug carriers based on physiological internal stimuli cannot efficiently orientate to designated locations. Therefore, it is necessary to introduce the self-propelled particles to the drug release of the microgels. In order to study self-propulsion of microgels induced by light, it is also a challenge to prepare micronsized microgels so that they can be observed directly under optical microscopes. In this work, phototactic microgels with photoresponsive properties are prepared. The microgel particles can be observed by confocal laser scanning microscopy. The photoresponsive properties of microgels are fully investigated by various instruments. Light can also regulate the state of the microgel solution, making it switch between turbidity and clarity. The phototaxis of particles irradiated by UV light was studied, which may be used for microgels enrichment and drug transportation and release.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2026
·
IF 2.4
Yuhang Chen,Long Cheng,Zhao Lei,Yuqi Su,Maogang He,Ying Zhang
Transport properties, including thermal diffusivity and the Fick diffusion coefficient, are key parameters for optimizing chemical processes and ensuring production safety. In this work, the thermal diffusivity and Fick diffusion coefficient of the n–heptane + n–decane binary mixture were measured by dynamic light scattering at n–heptane amount fractions from 0.25 to 0.75. Measurements were performed along five isobars at 0.1, 2.5, 5.0, 7.5, and 10 MPa. Thermal diffusivity was determined over 298.15–523.15 K, except at 0.1 MPa, where the temperature range was limited to 298.15–373.15 K, while Fick diffusion coefficients were obtained from 298.15 to 373.15 K. The relative expanded uncertainties in thermal diffusivity and Fick diffusion coefficient were estimated to be 0.094 and 0.146, respectively, at a confidence level of 0.95 (k = 2). The effects of temperature, pressure, and composition on thermal diffusivity and Fick diffusion coefficient were analyzed, aiming to offer guidance for optimizing the design of industrial processes and ensuring operational safety and reliability.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Huidan Li,Ye Liu,Jun Ding
Abstract Isothiocyanates (ITCs) are bioactive hydrolysis products of glucosinolates in Brassicaceae plants with diverse health-promoting properties. However, comprehensive profiling of ITCs remains challenging due to the limited availability of authentic standards, the underexplored structural diversity of naturally occurring ITCs, and poor mass spectrometry (MS) detection sensitivity. Here, we established a N,N-dimethylethylenediamine (DMEDA) derivatization strategy and constructed an in silico MS/MS library for systematic ITC annotation. DMEDA derivatization markedly improved LC–MS detection sensitivity, enabling the reliable detection of low-abundance ITCs with limits of detection at the nanomolar level. Detailed characterization of the MS/MS fragmentation behaviors of derivatized ITC standards revealed subclass-dependent fragmentation patterns, which were subsequently translated into spectral prediction rules for the generation of an in silico mass spectral library with 2209 simulated ITC structures. Application of the developed workflow to Brassicaceae plant samples enabled the annotation of 50 endogenous ITCs, including 4 low-abundance compounds that were previously not reported. Our work provides a valuable resource for the systematic exploration of ITC structural diversity and facilitates functional studies of glucosinolate-derived bioactive metabolites.
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JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 4.4
Liu Tong,Zhang Jie,An Qianqian,Wu Yusheng,Li Laishi,Fang Tiehui,You Junhua
Electrocatalytic water splitting is a clean and sustainable method for hydrogen production. Cobalt diselenide (CoSe 2 ) is an electrocatalyst with great application prospects. However, due to the inherent low electrical conductivity and aggregation tendency of transition metal selenides, their practical applications are limited. In this study, under the constraint of three-dimensional porous nitrogen-doped reduced graphene oxide/nickel foam (N-rGO/NF) substrates, small-sized nitrogen-doped cobalt diselenide (CoSe 2-x N x ) nanoneedles were prepared by a one-step hydrothermal method, and excellent electrocatalysts were obtained. The optimized catalyst (N-rGO/CoSe 2-x N x /NF-100) exhibits excellent OER performance in an alkaline environment at current densities of 10, 50 and 100 mA cm −2 . N-rGO/NF not only provides good electrical conductivity for the electrocatalyst, but also limits the size of CoSe 2 . The three-dimensional porous structure constructed by small-sized CoSe 2 nanoneedles increases the specific surface area of the electrocatalyst, which is conducive to the OER process. The doping of nitrogen in CoSe 2 forms Co-Nx electron transfer channels, accelerating charge transfer. Density functional theory (DFT) calculations have confirmed that structural modification methods such as limiting the size of the electrocatalyst to avoid agglomeration, as well as electronic control methods such as nitrogen doping, can accelerate the surface structure reconstruction process of the catalyst, thereby improving the adsorption energy of the electrocatalyst for OER intermediates and reducing the overpotential.
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高影响因子
BMC Nephrology
·
2025
·
IF 2.4
Chen Wenjie,Hou Suyue,Hu Jijia
Background This study investigates the molecular mechanisms by which di-n-butyl phthalate (DBP) and mono-n-butyl phthalate (MnBP)-induced diabetic kidney disease (DKD). Methods Differential expression analysis and Weighted Gene Co-expression Network Analysis were used to identify DKD-associated targets. Machine learning, molecular docking, molecular dynamics simulations, and public databases were integrated to explore the interaction between DBP/MnBP and target proteins. Results Six core genes were identified: DUSP1 , PTGS2 , FOSB , GDF15 , NR4A1 , and CXCR2 . Among these, DUSP1 and FOSB showed excellent performance in single-gene ROC curves, box plots, public databases, and molecular docking. Molecular docking and molecular dynamics simulations demonstrated a stable binding affinity between DBP/MnBP and the target proteins. Conclusion This research suggests that DBP/MnBP may promote DKD by targeting these six core genes. The binding capacity and stability of DBP/MnBP with these genes were confirmed by machine learning, molecular docking, and molecular dynamics simulations. These findings provide a direction for future in-depth research on the DBP/MnBP-induced DKD mechanism.
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高影响因子
Nanoscale
·
2022
·Vol. 15
·期 3
·1210-1220
·
IF 8.307
Shiang Li,Chao Feng,Yuehong Xie,Changyan Guo,Afaq Hassan,Jide Wang
The development of low-cost, long-term stability, and good oxygen reversible catalytic reaction (ORR/OER) and hydrogen evolution (HER) activity under the same electrolyte concentration of electrocatalytic materials has an important role in the construction of large-scale applications and more valuable sustainable energy systems. Among them, the representative CoMn–Nx@N–C-900-0.2 showed good ORR/OER/HER catalytic activity in 0.1 M KOH alkaline electrolyte, specifically manifested by its half-wave potential E = 0.84 V in the ORR test, which was better than that of commercial Pt/C. The total oxygen electrode activity index of OER/ORR was E = 0.79 V, and it also showed good HER performance. When the current density was 10 mA cm−2, the operating potential was E = −0.266 V. The synergistic effects of the CoMn bimetallic alloy, tubular layered porous structure, which exposed more active area and various nitrogen species such as CoMn–Nx, were the main reasons for the improvement of the trifunctional catalytic performance of electrocatalytic materials. The synthesis strategy and analysis of the electrocatalyst performance provide a new reference for the development of multifunctional materials with high catalytic performance.
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高影响因子
FUEL PROCESSING TECHNOLOGY
·
2022
·
IF 8.129
Qin Wen,Dongxu Tian,Zequan Zeng,Fei Qi,Zhanggen Huang
Fe-N-C catalysts with magnetism were successfully synthesized through one-step heat treatment of ferrosoferric oxide and covalent organic frameworks composite (Fe 3 O 4 @COF) as heterogeneous catalysts for persulfate (PS) activation and 2,4-dichlorophenol (2,4-DCP) degradation. The morphologies, compositions and active sites were adjusted by changing the pyrolysis temperature. The catalyst prepared at 900 °C showed excellent activity with 2,4-DCP removal of 90% in 60 min, recycling convenience and regeneration stability. Large surface area (342 m 2 g −1 ) and hierarchical porous structure benefited PS activation. It was proved that the radical and non-radical mechanisms coexisted in Fe-N-C-900/PS system with the results of quenching and electrochemical experiments. Roles of graphitic N and Fe-N X were distinguished. Graphitic N and Fe-N X sites palyed an prominent part in the non-radical activation process , in which Fe-N X was closely related to the electron transfer activation mechanism. Density functional theory (DFT) calculation further verified that the Fe N 2 was the key site for PS activation. 2,4-DCP degradation was proved to be a toxicity-attenuation process according to the biotoxicity analysis. The effects of several operating parameters and degradation of different model pollutants were also systematically evaluated, and possible activation mechanism was proposed.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Long-Qi Yang,Xiao-Ming Jiang,Shao-Min Pei,Wen-Fa Chen,Bin-Wen Liu,Guo-Cong Guo
Exploring new infrared nonlinear optical (IR NLO) materials with superior overall properties is scientifically and technically important. However, large second-order harmonic generation (SHG) efficiencies and high laser-induced damage thresholds (LIDT) are incompatible, which makes realizing this goal a challenge. The IR NLO performance of an A-NIIB-MIIIA-Q (Q: chalcogen) system was optimized by simultaneously modulating A/(M + N) and M/N ratios (A: alkali metal; N, M: tetra-coordinated metals), and SHG–LIDT balance was achieved. Three new sulfides, KCd3Ga5S11 (1), RbCd4Ga3S9 (2), and Cs2Cd2Ga8S15 (3), containing the same CdS4 and GaS4 but with different A/(Ga + Cd) and Ga/Cd ratios were obtained. Among these compounds, compound 3 exhibits both the largest SHG efficiency (0.5 × AgGaS2) and LIDT (35 × AgGaS2), which can be ascribed to the Ga/Cd modulation for enhancing the NLO functional motif distortions and SHG efficiency as well as the A/(Ga + Cd) modulation for enlarging the band gap and LIDT. Remarkably, compound 3 is the first phase-matchable IR NLO material in the A-NIIB-MIIIA-Q family. This article proposes a novel avenue to explore infrared nonlinear materials with superior comprehensive properties by modulating the A/(M + N) and M/N ratios.
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