COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Qingling Nie,Peipei Xu,Jiajia Liu,Yanhanzhuo Wang,Qian Huang,Ruiyun You,Yunzhen Liu,Lizhi Li,Yudong Lu
To improve the sensitivity, accuracy and specificity of the assay, a two-dimensional silver substrate with EF=5.85×10 8 was first synthesized as a SERS substrate, on the surface of which DSP molecules were modified to form a DSP-antibody coupling through the activation of two N-hydroxy succinimide (NHS) esters to capture TNF-α. Subsequently, aptamerized silver-coated gold nanospheres (Au@TFMBA@Ag) were synthesized as Surface-Enhanced Raman Scattering (SERS) recognition probes. These probes were employed to create a sandwich structure for the quantitative detection of Tumor Necrosis Factor-alpha (TNF-α), utilizing the SERS signal intensity at 1374 cm −1 . Quantitative detection of TNF-α was successfully accomplished within the concentration range of 10 −4 to 10 −10 mg·mL −1 . Clinical serum samples were collected and subjected to testing. Significance analysis, conducted through the T-test (p < 0.0001), unequivocally showed the method's ability to differentiate between sera from normal individuals and those diagnosed with colon cancer.
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TALANTA
·
2025
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IF 6.1
Liwei Hou,Xinyue Xu,Fengchun Tian,Yi Xu
The effective qualitative and quantitative detection of mixed components of volatile organic compounds (VOCs) with similar molecular structures has always been a challenge and hotpoint in the research. A novel quartz-crystal microbalance (QCM) nanocomposite sensor integrated with a surface-enhanced Raman scattering (SERS) detection platform for multi-component gas analysis was proposed and fabricated in this paper. MIL-100 (Fe)/PAN composite fibers were developed on QCM via electrospinning of polyacrylonitrile (PAN) and hydrothermal synthesis, addressing the integration issues of MIL-100 particles in devices while maintaining high specific surface area. The MIL-100(Fe)/PAN@QCM sensor can simultaneously collect QCM frequency responses and SERS spectra, enabling the quantitative and qualitative detection of toluene and benzaldehyde in mixtures. The linear response relationship between the frequency obtained by the QCM sensor and the concentration of toluene and benzaldehyde gases was in the range of 0–100 ppm, and the limits of detections were 0.57 and 0.86 ppm, respectively. Additionally, an algorithm, that considered the relationship among the QCM frequency response signal, intensity values of the characteristic peaks in the SERS spectra, and the gas concentration, was developed and applied to measure the mixed gases of toluene and benzaldehyde in the concentration range of 0–100 ppm. The relative standard deviations (RSDs) of spiked sample determinations were found to be in the range of 3–8%. This work provided a new method for the efficient and rapid detection of gas molecules with similar structures, which could be extended and applied to detect more multi-component gas mixtures.
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Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 44
·11631-11639
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IF 5.256
Shunsheng Ye,Hongyu Wang,Hailong Wang,Lingxia Chang,Junhu Zhang,Bai Yang
A large-scale, uniform array of particle-in-aperture (PIA) hybrid architectures for highly reproducible surface-enhanced Raman spectroscopy (SERS) is rationally designed and prepared via electrostatic self-assembly of Au nanospheres and vertical deposition of Ag. By controlling the thickness of the Ag coating, polarization-independent ring-shaped hot spots with tunable width can be created around individual nanospheres, offering far higher Raman enhancements than a bare Au nanosphere array and a flat Ag film do. Three PIA substrates with different Ag thicknesses and an identical nanosphere diameter of 58 nm were investigated. Consequently, it is found that a 28 nm Ag coating enables the wavelength matching between the plasmonic coupling band and the laser line, leading to an enhancement factor of up to 4.5 × 106. Meanwhile, remarkable reproducibility of SERS signals is realized by ruling out the impact of anomalous hot spots. To this end, three guidelines are meticulously followed, i.e. using monodisperse Au nanospheres, distributing nanoring cavities evenly onto the substrate, and decoupling adjacent nanoparticles by isolating them far away from each other. This work offers a reliable strategy to fabricate high-performance SERS substrates in a simple, cost-effective and scalable manner, paving the way for practical sensing applications, such as quantitative SERS measurements.
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MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Zhilong Chen,Yan Guo,Xinyue Gu,Xinyu Liu,Jingjing Zhang,Chunyuan Song,Lianhui Wang
Interstitial fluid (ISF) is a prevalent accessible and information-rich biofluid, and monitoring pH level of interstitial fluid plays a crucial role in the physiological health assessment. However, facile extraction of ISF and sensitive detection of pH is still a great challenge. Herein, a flexible plasmonic microneedle array (PMNA)-based SERS sensor was developed for facile and sensitive pH monitoring of skin ISF. The flexible microneedle arrays were fabricated by norland optical adhesive 65 (NOA65), and coated with pH-sensitive 4-Mercaptobenzoic acid (4-MBA)-labeled silver nanoparticles (Ag NPs) by electrostatic assembly. The proposed flexible PMNA-based SERS sensor has a good linear response in the pH range of 5 to 9 which covers the normal pH level in skin interstitial fluid. The ISF extracted from porcine skin was chosen as the test model, and the pH levels detected by the PMNA-based SERS sensors were well consistent with the theoretical results. Besides, the proposed flexible SERS sensors show good mechanical robustness, sensing stability, and biocompatibility before and after skin insertion, which can be a potential analytical tool for practical detection of pH levels in ISF and provide technical support for SERS-based wearable biosensing.
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CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Ning Bi,Lixiao Sun,Meihua Hu,Wei Song,Jun Xu,Lei Jia
A novel colorimetric/surface-enhanced Raman spectroscopy (SERS) dual-signal nanozyme sensor was developed for the rapid and sensitive detection of Hg 2+ based on peroxidase-like gold-platinum core–shell nanoflowers (Au@Pt NFs). Au@Pt NFs catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue oxidation product, 3,3′,5,5′-tetramethylbenzidinediamine (oxTMB), which exhibits high Raman activity. However, the presence of Hg 2+ inhibited the catalytic activity of Au@Pt NFs, leading to a gradual disappearance of the blue color of oxTMB. Consequently, both the absorbance at 650 nm and the Raman intensity at 1639 cm −1 decrease with increasing Hg 2+ concentration. The limits of detection (LODs) were 3.2 nM for colorimetric and 0.22 nM for SERS. The preparation method of Au@Pt NFs dual-signal nanozyme sensor was simple and allowed for naked-eye colorimetric detection as well as simultaneous SERS signal measurement. In addition, this nanozyme sensor demonstrates excellent selectivity and sensitivity, making it a promising probe for water quality monitoring.
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TALANTA
·
2024
·
IF 5.6
Wei Li,Shijie Dai,Xiang Li,Qianjin Li,Jianlin Li
The stability, reproducibility and engineering of SERS substrate faces a great challenge in melamine SERS assay. In this work, a simple, highly sensitive, stable and cost-efficient SERS detection platform for melamine was established based on its Raman fingerprints spectrum. The Ag@ porous silicon photonic crystal (Ag@PPC) was prepared as the 3D SERS substrate by electrochemical etching and magnetron sputter technology. The main influence factors for the preparation of SERS substrate were investigated in detail. The analytical enhancement factor of the 3D SERS substrate can reach to 2.6 × 10 8 . The 3D SERS detection platform showed a wide linear detection range of 10 −4 ∼10 mg L −1 and a low limit of detection of 0.1 μg L −1 for melamine. Moreover, such detection platform showed good stability, high reproducibility and high recovery rates for melamine. The 3D Ag@PPC SERS substrate can be easily prepared and engineered, displaying a great potential application in food safety field.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Ruoyu Zhang,Shan Li,Baoru Liu,Xinyu Yang,Weijun Kang,Lianfeng Ai
In this study, we designed and prepared a novel, stable, and sensitive membrane-based surface-enhanced Raman scattering (SERS) substrate based on the synergistic interaction of two-dimensional MXene material Ti 3 C 2 Tx and silver nanoparticles (AgNPs). Using this AgNPs/Ti 3 C 2 Tx active substrate, a rapid SERS method for determination of paraquat residues in vegetables and fruits was developed. The AgNPs/Ti 3 C 2 Tx films were formed by dropping AgNO 3 solution onto the film and drying, which was synthesized by wet chemical method , and then filtered into an exfoliated Ti 3 C 2 Tx film. The Ti 3 C 2 Tx film can not only effectively enrich analytes, but also provide charge transfer resonance enhancement. The homogeneous AgNPs particle size of 40–120 nm on the substrate surface of Ti 3 C 2 Tx can effectively enhance the Raman signal intensity, and the enhancement factor of methylene blue can reach 10 5 . The detection limit of the detection method for paraquat in 9 fruit and vegetable matrices was lower than 3.0 μg/kg, and the limit of quantification was lower than 12 μg/kg. The results suggest that AgNPs/Ti 3 C 2 Tx platform could be used as a sensitive and reliable for food safety analysis based on SERS method.
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Small
·
2024
·
IF 13
Tao Yang,Jianping Zhou,Yongkang Wang,Ben Fan,Jing Qiao,Lixiang Chen,Xiaoyu Wang,Lingxiang Guo,Hong Yang,Quan Li
Surface-enhanced Raman scattering (SERS) is widely used in all kinds of detection due to its ultrahigh sensitivity and selectivity. Micromotors, when used as SERS sensors, or the so-called “hotspots on the fly”, can combine both controlled mobility and SERS sensing capacity, and are ideal for versatile in situ detection. In this work, mobile SERS sensors are successfully fabricated by growing gold nanospikes onto magnetic microsphere surfaces. These mobile micromotors can act as normal SERS sensors, characterized by the trace detection of thiram, a highly toxic fungicide. The detection limit can reach 0.1 n M , as good as most other noble metal deposited substrates. With significant magnetic gradient forces, separation of pathogenic bacteria from bulk solution is achieved once these magnetic micromotors bind with bacterial cells. Manipulated propulsion of micromotors, on the other hand, enables them to approach and contact pathogenic bacterial cells on command and further acquire Raman spectra under a controlled degree of contact, a capability never seen with passive sensors. The robotic SERS sensors have demonstrated unique sensing characteristics with controlled manipulations along with discriminative detection between bacterial species.
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TALANTA
·
2024
·
IF 5.6
Zhaoyan Yang,Kai Zhu,Kuo Yang,Yeming Qing,Youjiang Zhao,Lei Wu,Shenfei Zong,Yiping Cui,Zhuyuan Wang
Nanoplastics present a significant hazard to both the environment and human health. However, the development of rapid and sensitive analysis techniques for nanoplastics is limited by their small size, lack of specificity, and low concentrations. In this study, a surface-enhanced Raman scattering (SERS) chessboard substrate was introduced as a multi-channel platform for the pre-concentration and detection of nanoplastics, achieved by polydomain aggregating silver nanoparticles (PASN) on a hydrophilic and a punched hydrophobic PVDF combined filter membrane. Through a straightforward suction filtration process, nanoplastics were captured by the PASN gap in a single step for subsequent SERS detection, while excess moisture was promptly eliminated from the filter membrane. The PASN-based SERS chessboard substrate, benefiting from the enhanced electromagnetic (EM) field, effectively discriminated polystyrene (PS) nanoplastics ranging in size from 30 nm to 1000 nm. Furthermore, this substrate demonstrated favorable repeatability (RSD of 8.6 %), high sensitivity with a detection limit of 0.001 mg/mL for 100 nm of PS nanoplastics, and broad linear detection ranges spanning from 0.001 to 0.5 mg/mL (R 2 = 0.9916). Additionally, the SERS chessboard substrate enabled quantitative analysis of nanoplastics spiked in tap and lake water samples. Notably, the entire pre-concentration and detection procedure required only 3 μL of sample and could be completed within 1 min. With the accessibility of portable detection instruments and the ability to prepare substrates on demand, the PASN-based SERS chessboard substrate is anticipated to facilitate the establishment of a comprehensive global nanoplastics map.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Long Wu,Luyao Jiao,Danni Xue,Yueqing Li,Yu Han,Wei Ouyang,Qi Chen
Microcystin-LR (MC-LR), a potent cyanotoxin in freshwater, poses a risk of severe liver damage and other health issues, making its detection vital. However, the detection capabilities of conventional antibodies are constrained, which limited their use in immunoassays. In this work, we designed a new bifunctional nanobody, named A2.3-SBP (comprised of nanobody and streptavidin binding peptide), capable of binding with MC-LR and streptavidin. Based on A2.3-SBP and Fe 3 O 4 @Au-Pt nanozyme, we introduced an enzyme-free immunosensor that operated in microplate with colorimetric and surface-enhanced Raman scattering (SERS) detection modes. The dual-mode assay showed color changes and SERS intensity directly correlating to MC-LR concentrations with a range from 1.0 to 500 ng/mL and a limit of detection of 0.26 and 0.032 ng/mL, respectively. This strategy eliminated the need for complex enzymatic reactions and realized dual-signal detection of MC-LR in 96 water samples (0.03 μg/kg) within 30 min, suggesting its potential in drinking water detection.
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TALANTA
·
2024
·
IF 5.6
Zhihao Li,Zhuang Ding,Zilong Yan,Konghao Han,Maofeng Zhang,Hongyang Zhou,Xu Sun,Hui Sun,Jianhua Li,Wei Zhang,Xiaohong Liu
The ubiquity of plastic products has led to an increased exposure to micro and nano plastics across diverse environments, presenting a novel class of pollutants with substantial health implications. Emerging research indicates their capacity to infiltrate human organs, posing risks of tissue damage and carcinogenesis. Given the prevalent consumption of beverages as a primary vector for these plastics' entry into the human system, there is an imperative need for the advancement of precise detection methodologies in liquids. In this study, we introduce a substrate comprising a Nickel Oxide (NiO) nanosheet array decorated with Silver Nanoparticles (AgNPs) for the Surface-Enhanced Raman Spectroscopy (SERS) analysis of micro//nano plastics. This configuration, leveraging a unique nanowell architecture alongside silver plasmonic enhancement, demonstrates unparalleled sensitivity and repeatability in signal, facilitating the accurate quantification of these contaminants. Through the application of a portable Raman apparatus, this study successfully identifies prevalent micro/nano plastics including polystyrene (PS), polyethylene (PE), and polypropylene (PP), achieving detection sensitivities of 5 μg/mL, 25 μg/mL, and 25 μg/mL, respectively. Moreover, the substrate's efficacy extends to the detection of PS within commonly consumed beverages such as water, milk, and liquor with sensitivities of 25 μg/mL, 50 μg/mL, and 50 μg/mL, respectively. These findings highlight the substrate's potential as an expedient and effective sensor for the real-time monitoring of micro/nano plastic pollutants.
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CHEMISTRY OF MATERIALS
·
2018
·
IF 9.89
Xuefei Lu,Youju Huang,Baoqing Liu,Lei Zhang,Liping Song,Jiawei Zhang,Afang Zhang,Tao Chen
The two-dimensional (2D) monolayer gold nanoparticle (Au NP) film is of significant interest and importance in both fundamental and practical applications including optoelectronic devices, sensing, catalysis, and surface-enhanced Raman spectroscopy (SERS). Because of the weak physical interaction, the conventional monolayer Au NP film fabricated at the oil–water interface was unstable, easily breakable, and difficultly transferred. In the present work, we report on a simple and effective chemical cross-linking strategy at the air–water interface to achieve a large-scale monolayer gold nanoparticle film with intelligently tunable size of nanogaps, and excellent free-standing and easily transferable properties. In our strategy, acrylamide, a polymerizable molecule, was first modified on the surface of Au NPs for subsequent self-assembly into a monolayer film at the liquid–liquid interface. Through photopolymerization of acrylamide, a chemically cross-linked film was formed with closely packed nanoparticles, highly macroscopic uniformity, and excellent free-standing property, which allowed it to be easily transferred from the air–water interface onto various solid substrates while maintaining its integrity. It is interesting to find that the macroscopic film underwent an in situ shrinkage under irradiation of UV-light, and its area shrinkage ratio is close to 55% (equal to 2.2 times) of that from non-cross-linked counterparts. More importantly, UV-light-controlled in situ shrinkage of the Au NP film would lead to intelligently, precisely tuned nanogaps less than 0.5 nm between neighboring Au NPs for maximal amplification of SERS signals, and the macroscopic uniformity of the films ensured the reproducible performance of SERS signals, providing an ideal candidate for SERS applications.
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