Food Frontiers
·
2025
·
IF 6.9
Sibo Zhao,Jielin Jiang,Yan Liu,Mianhong Xu,Rui Yang,Zhi Yang,Jilin Xie,Zhanggui Ding,Liyong Luo,Liang Zeng
Excessive alcohol consumption in contemporary society induces alcoholic liver disease (ALD). Previous laboratory studies demonstrated hepatoprotective and gut microbiota–modulating effects of ripe Pu-erh tea. To identify the key bioactive components responsible for these effects and to support the development of functional beverages, we investigated the preventive effects of various membrane-separated Pu-erh tea extracts on a chronic ALD mouse model. The extracts included an 800-Da nanofiltration fraction, a fraction isolated via a 50-nm ceramic membrane, the retentate from the 50-nm ceramic membrane filtration, and unfiltered Pu-erh tea. The results revealed that the fraction isolated via a 50-nm ceramic membrane (T4) significantly improved alcohol-induced intestinal barrier dysfunction, reduced lipopolysaccharide (LPS) release, and suppressed the release of proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). Furthermore, T4 attenuated hepatic lipid accumulation, oxidative stress, and inflammatory responses by modulating the Nrf2/NQO1 antioxidant pathway and inhibiting toll-like receptor 4 (TLR4)/MyD88-mediated proinflammatory signaling. Notably, T4 restored microbial homeostasis by increasing the relative abundance of beneficial bacteria, such as Dubosiella , Muribaculum , and Lactobacillus , and significantly regulated tryptophan metabolism and its related metabolite, indole-3-acetic acid (IAA). Overall, the membrane separation technology enriched T4 with polyphenols and flavonoids, which further regulated tryptophan metabolism by regulating liver oxidative stress and promoting probiotic colonization and ultimately alleviates ALD. The membrane-fractionated Pu-erh tea, particularly T4, represents a promising functional beverage to combat alcohol-induced metabolic disorders.
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高影响因子
eFood
·
2023
·Vol. 4
·期 2
·e64
·
IF 0
Yuhong Li,Xiaoling Chen,Yueli Lin,Yaqi Yang,Li Zhang,Pengcheng Zhao,Chenxi Wang,Junjie Fei,Yixi Xie
Catechin (Cat) is one of the primary functional components of tea, and its accurate detection is significant for understanding the health benefits of tea. In this study, cyclodextrin nanosponges (CDNS) were prepared and employed to combine with graphene quantum dots (GQDs) and carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) to form a CDNS/GQDs/MWCNTs-COOH composite. Scanning electron microscope and X-ray diffraction were used to characterize the composite which was used to build a new electrochemical Cat sensor. The abundance of cavities, intermolecular gaps, and surface active groups of CD molecules endowed CDNS with remarkable Cat enrichment capacity. Due to the outstanding biocompatibility of GQDs, the good electrocatalytic activity of MWCNTs-COOH, and the enrichment capacity of CDNS, the composite modified glass carbon electrode (GCE) exhibited excellent electrochemical sensing performance. The CDNS/GQDs/MWCNTs-COOH/GCE showed a sensitive peak current response to catechins in the linear range between 2 × 10 −8 and 1.2 × 10 −6 M, with a minimum limit of detection (S/N = 3) of 4.45 nM. The sensor has also been successfully applied for catechin detection in tea beverages.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yiwen Lou,Wanqin Chen,Yating Gan,Ying Zhou,Haipeng Ye,Weiwei Huan,Yiming Zhang
A high-performance lateral flow and paper-based microfluidic device ( μ PAD) was constructed to develop a rapid read-out analytical platform for heavy metal elements (Pb (II)) in green tea with complex matrices. The entire analytical device was integrated with several functional parts, including a liquid flow channel and an enrichment visualization zone prepared by the molecularly imprinted polymer (MIP) protocol. Under the optimal conditions, the Pb (II) content in tea products can be sensitively and specifically analyzed using an in situ visualization readout. Herein, a rapid visual read-out analysis of the lead level can be achieved by overcoming the other co-existing interfering elements in the traditional green tea sample. The ionic imprinted paper chip can effectively capture the Pb (II) of the digested sample solution, and a visualization sensing process occurred between the metal elements and a chelating visual reagent with high sensitivity and selectivity in situ . Owing to the stable visualization performance, the constructed chip demonstrated good contrast, with a low background and easily recognizable colored spots. The linear range was 2.5–100 mg kg −1 , and the limit of detection for Pb (II) in traditional green tea was 1.375 mg kg −1 . In addition, other co-existing metal elements did not interfere with the analysis because of the appropriate pretreatment and selectivity of the paper-based chip. Furthermore, the analytical results for tea samples were verified by atomic absorption spectrophotometer (AAS) tests, which demonstrated the robustness and reliability of the proposed method. Therefore, the high-performance visualization lateral flow- μ PAD is a promising platform for the rapid analysis of metal element levels in food products.
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高影响因子
Heliyon
·
2023
·
IF 4
Xiao-Lan Yu,Jia Li,Yanqing Yang,Jiayi Zhu,Haibo Yuan,Yongwen Jiang
In this study, four kinds of Longjing tea, the famous flat green tea and the protected geographical indication product in China, were used to explore the quality difference of the same green tea due to the cultivar, geographic origin, and storage time under the premise of consistent picking conditions and processing technology using the widely targeted metabolomics. Results showed that 483 flavonoid metabolites with 10 subgroups of flavonoids were screened and 118 differential flavonoid metabolites were identified. The number and subgroups of differential flavonoid metabolites produced by different cultivars of Longjing tea were the largest, followed by storage time, and third by the geographic origin. Glycosidification and methylation or methoxylation were the main structural modifications of differential flavonoid metabolites. This study has enriched the understanding of the effects of the cultivar, the geographic origin, and the storage time on the flavonoid metabolic profiles of Longjing tea, and provided worthy information for the traceability of green tea.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qiu-Yue Ma,Qian-Da Xu,Nan Chen,Wei-Cai Zeng
Plant-based proteins represent a more sustainable alternative, the approaches to modify and enhance their functionality and application are focused on. Covalent interaction could significantly modify the structure and function properties of protein. This study investigated the effects of covalent interaction between wheat gluten and tea polyphenols on the structure, aggregation, stability, and digestive properties of their covalent complex, as well as the possible action mechanism. The results showed that tea polyphenols could interact with gluten via covalent bonds (C N and/or C S), while tea polyphenols also acted as a bridge connecting gluten molecules, thus making covalent complex to show the larger particle sizes. This covalent interaction significantly changed the secondary structure, tertiary structure, and surface hydrophobicity of gluten. Moreover, covalent complex exhibited the high polyphenols bioaccessibility during in vitro digestion. The peptide bonds of covalent complex were mainly broken in gastric digestion, while the covalent bonds between tea polyphenols and gluten were completely destroyed in intestinal digestion. In addition, their digestates exhibited excellent antioxidant capability. All results suggest that wheat gluten have potential to prepare functional carrier for transporting active compounds and protecting them during digestion.
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PubMed
高影响因子
Journal of Functional Biomaterials
·
2025
·Vol. 16
·期 4
·124
·
IF 5.2
Wei Shen,Qilian Ye,Hongbo Zhang,Shenghong Xie,Shiqi Xie,Cailian Chen,Jinying Liu,Zhengwei Huang,Hai-Bin Luo,Ling Guo
Psoriasis is a chronic and recurrent inflammatory disease driven not only by intrinsic factors such as immune system dysregulation but also by external factors, including bacterial infections. In contrast to the control of a single pathogenic pathway, combination therapies addressing both the immune and infectious components of psoriasis pathogenesis may offer a more effective strategy for controlling its progression. In this study, we developed a sprayable hydrogel incorporating tea polyphenol-loaded lauric acid liposomes (TP@LA-Lipo gel) to investigate its anti-inflammatory and antibacterial role in psoriasis. Our results demonstrated that TP@LA-Lipo modulated macrophage activity, reduced the expression of iNOS and TNF-α, and remodeled the immune microenvironment. Meanwhile, TP@LA-Lipo effectively eliminated Staphylococcus aureus and Escherichia coli through membrane disruption, mitigating the provoked inflammatory response. More importantly, TP@LA-Lipo gel, when sprayed onto the psoriasis lesions, provided sustained drug release over three days, enabling deeper penetration through the thickened stratum corneum to reach the inflamed layers beneath. Furthermore, in an imiquimod-induced psoriasis mouse model, TP@LA-Lipo gel effectively restored the damaged skin, alleviated histopathological changes, and reduced the systemic immune response. In summary, these findings indicate that TP@LA-Lipo gel offers a comprehensive strategy for effective disease management and improving the quality of life for psoriasis patients.
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PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Yixuan Wang,Shijia Fu,Yao Zeng,Siwen Jiao,Guihong Chai,Yuehong Xu
5-aminolevulinic acid photodynamic therapy (ALA-PDT) is an emerging therapeutic strategy for skin cancer due to its noninvasiveness and high spatiotemporal selectivity. However, poor skin penetration, poor intratumoral delivery, the instability of aqueous ALA, and the tumor’s inherent hypoxia microenvironment are major hurdles hindering the efficacy of ALA-PDT. Herein, we aim to address these challenges by using microneedles (MNs) to assist in delivering nanoparticles based on natural polymeric tea polyphenols (TP NPs) to self-assemble and load ALA (ALA@TP NPs). The TP NPs specifically increase cellular uptake of ALA by A375 and A431 cells and reduce mitochondrial membrane potential. Subsequently, the photosensitizer protoporphyrin IX derived from ALA accumulates in the tumor cells in a dose-dependent manner with TP NPs, generating reactive oxygen species to promote apoptosis and necrosis of A375 and A431 cells. Interestingly, TP NPs can ameliorate the tumor’s inherent hypoxia microenvironment and rapid oxygen consumption during PDT by inhibiting hypoxia inducible factor-1α, thereby boosting reactive oxygen species (ROS) generation and enhancing ALA-PDT efficacy through a positive feedback loop. After ALA@TP NPs are loaded into MNs to fabricate ALA@TP NPs@MNs, the MNs enhance skin penetration and storage stability of ALA. Importantly, they exhibit remarkable antitumor efficacy in A375-induced melanoma and A431-induced squamous cell carcinoma with a reduced dose of ALA and reverse hypoxia in vivo . This study provides a facile and novel strategy that integrates MNs and green NPs of TP for addressing the bottlenecks of ALA-PDT and enhancing the ALA-PDT efficacy against skin cancers for future clinical translation.
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yongjia Cheng,Yao Chen,Jianjun Liu,Dongyang Li,Juan Zhang,Chaojie Li,Wen Yang,Zhiwei Lei
Plants release a variety of defensive volatiles in response to biotic stress. The present study examined interactions between Colletotrichum fructicola and the defense volatiles induced by this fungal species in the tea cultivar “Qiancha No. 1”. Analysis of the volatiles emitted from C. fructicola-infected leaves of “Qiancha No. 1” revealed that four volatiles showed a relative content increase of more than 2%. Fungicidal activity assays demonstrated that decanal exhibited the strongest antifungal activity among the four volatiles. Further physiological experiments demonstrated that the antifungal mechanism of decanal was associated with disruption of the cell wall and the membrane. Transcriptome analysis revealed that the genes encoding Chitinase 1, chitin synthase 1, and endochitinase42 were identified as potential targets that may be involved in cell wall degradation by decanal. Additionally, genes encoding cytochrome P450-DIT2 and multidrug resistance protein fer6 were identified as potential targets that may be associated with membrane damage. This study is the first to demonstrate that decanal acts as a direct defense volatile in the interaction between C. fructicola and “Qiancha No. 1,” highlighting its potential as an effective antifungal agent.
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高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Li Zhen,Qian Wang,Huimin Cui,Yifen Wang,Jianxin Cao,Yaping Liu,Hesham R El-Seedi,Guiguang Cheng
Goji ( Lycium barbarum L.) leaves are widely processed as a healthy tea (GT) in China for their multiple biological activities. In this study, different polar segments from GT ethanolic extract were obtained using the liquid-liquid fractionation method. n -Butanol fraction (GN) had the highest antioxidant activity while ethyl acetate fraction (GE) showed the highest inhibitory effect on the α -glucosidase enzyme. The anti-diabetic effect of GE was further investigated in type 2 diabetic mellitus (T2DM) mice. The oral administration of GE (300 or 600 mg/kg) reduced fasting blood glucose (FBG) levels, improved glucose tolerance ability, and ameliorated insulin resistance (IR) in T2DM mice. GE intervention could enhance the cholesterol transportation in the serum in coincidence with decreased total cholesterol, triglyceride levels, and non-esterified fatty acid. Transcriptomic, immunofluorescence and western blotting analysis revealed that GE significantly up-regulated insulin receptor substrate 1 (IRS1), phosphatidylinositol-3-kinase (PI3K), protein kinase B (AKT), glycogen synthesis (GS), and glucose transporter 4 (GLUT4). AMP-activated protein kinases (AMPK)-mediate gluconeogenesis and glycogenolysis, such as phosphoenolpyruvate carboxy kinase (PEPCK) and glucose-6-phosphatase (G6Pase) in the liver and kidney tissues of GE groups were decreased. Thus, GE alleviated the liver, kidney, and pancreatic tissue damage. The phytoconstituents of GE were characterized, and rutin, neochlorogenic acid, and its acylated derivatives have hypoglycemic effects. Furthermore, these findings suggested that Goji tea might be used as a promising hypoglycemic candidate.
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高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2025
·
IF 8.4
Yuliang Tu,Jianle Wang,Yanfeng Huang,Hong Deng,Xueming Liu,Jiaxin Lv
Hexavalent chromium [Cr(VI)] presents substantial environmental and health hazards due to its pronounced toxicity and mobility in soils. Traditional chemical reduction methods for Cr(VI) are often inadequate for treating chromium-contaminated soils, as they may fail to fully reduce Cr(VI), leading to incomplete remediation. Consequently, there is a critical need to develop more efficient and environmentally sustainable methods for the treatment of hexavalent chromium. This study explores an innovative approach using microwave-enhanced reduction of soil iron minerals facilitated by tea polyphenols to achieve transformational fixation of Cr(VI). The microwave-assisted treatment (0.5 wt% TP combined with MW at 700 W for 10 min) significantly reduced Cr(VI) concentrations in iron-rich soil from 363.4 mg kg −1 to 0.3 mg kg −1 , achieving a removal rate of 99.9%, compared to TP treatment alone (0.5 wt%). Transmission electron microscopy (TEM), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) analyses revealed that the enhanced removal efficiency of Cr(VI) by microwave-assisted tea polyphenols is attributable to the accelerated reduction of soil iron minerals by the polyphenols. Furthermore, microwave enhancement facilitated the transformation of Cr into more stable forms, such as Cr 2 O 3 and FeCr 2 O 4 . Additionally, the Cr(VI) content in the treated soil remained below 3 mg kg −1 after 360 days of atmospheric exposure, indicating sustained immobilization. This study demonstrates that the integration of tea polyphenols with microwave irradiation constitutes a promising strategy for the remediation of Cr(VI)-contaminated soils.
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PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Zhi-Peng Cheng,Yao-Zeng Zhang,Zhong-Zheng Guan,Rui-shan Liu,Zhen-Ming Lu,Li-Juan Chai,Hong-Yu Xu,Jin-Song Shi,Xiao-Juan Zhang,Zheng-Hong Xu
To improve the nutritional and sensory qualities of black tea extract, this study explores the potential of lactic acid bacteria (LAB) fermentation to enhance flavor and bioactive polyphenols through microbial metabolism and enzymatic transformation.Twenty-four LAB strains were screened for their growth, acid production, sugar consumption, and phenol metabolism. Three strains (LPL048, LPL043, LPL007) were identified as optimal for fermentation. These strains significantly increased the content and variety of volatile substances and enriched polyphenol content, with catechin, epicatechin, and epigallocatechin levels rising by 33%–73%, and phenolic acids (ferulic, caffeic, sinapic acids) increasing by 16%–75%. Quercetin levels rose by 9%–18%, and indole-3-lactic acid increased by 50%–119% post-fermentation. Fermentation enhanced the aftertaste of bitterness and astringency, enriched the flavor profile, and improved sensory complexity. This research provides a foundation for developing specialized LAB strains for black tea fermentation, potentially enhancing the health benefits and sensory experience of fermented tea products.
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高影响因子
TENSIDE SURFACTANTS DETERGENTS
·
2025
·
IF 1.2
Wenwei Wu,Wenhuan Huang,Zhiguo Sun,Yuqin Tian,Yangwen Zhu,Quan Shi,Zhen Tao,Qingchun Deng,Haoye Cui,Bingang Chen,Yaoguo Wang,Bo Yu
Emerging carbon capture, utilization, and storage (CCUS) technologies have driven the development of advanced nanocomposite materials. In this study, a novel polyhedral oligomeric silsesquioxane (POSS)-based nanomaterial, referred to as FAP, was synthesized using acrylamide, sodium α-olefin sulfonate, and tea saponin as monomers. The structure of the material was confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and silicon nuclear magnetic resonance spectroscopy ( 29 Si NMR), revealing a well-defined cage-like cubic architecture. TEM images clearly displayed the cage-like structure of the POSS material in FAP, while energy dispersive spectroscopy (EDS) confirmed the presence of silicon (Si) elements. Thermogravimetric analysis (TGA) showed that the incorporation of POSS materials improved the thermal resistance of FAP. The nanomaterials significantly improved the foaming performance and foam stability. The foam generated by FAP reached a height of 480 mL, with a half-life of 10 h under conditions of 120 °C and a salinity of 100,000 mg L −1 , showing excellent properties. Furthermore, the foam was regenerable in the presence of 20 % kerosene and maintained good foaming performance. Microscopic mechanism analysis indicated that the POSS nanomaterials compressed the electrical double layer of the polymer, reduced the particle size, increased the liquid film thickness, and markedly improved the foam stability. Under the influence of activators, the gel viscosity of FAP exceeded 100 mPa s, which significantly improved the underground sealing efficiency of CO 2 . The novel nanocomposite material exhibited integrated capabilities for CO 2 foaming, foam stabilization, and storage. The optimal concentration of the FAP foaming agent was 0.75 %, resulting in a 32.75 % increase in recovery efficiency, a resistance factor of 18.9, and a CO 2 storage rate of 12.59 %. The development of this nanocomposite material provides technological support for effectively mitigation of CO 2 channeling during CO 2 flooding and promotes the wider application of CO 2 flooding technology.
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高影响因子
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