MICROCHIMICA ACTA
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2024
·Vol. 191
·期 8
·1-10
·
IF 5.3
Wang Panxue,Liu Ying,Li Xiang,Li Cen,Li Guoliang
A ratiometric SERS aptasensor based on catalytic hairpin self-assembly (CHA) mediated cyclic signal amplification strategy was developed for the rapid and reliable determination of Escherichia coli O157:H7. The recognition probe was synthesized by modifying magnetic beads with blocked aptamers, and the SERS probe was constructed by functionalizing gold nanoparticles (Au NPs) with hairpin structured DNA and 4-mercaptobenzonitrile (4-MBN). The recognition probe captured E. coli O157:H7 specifically and released the blocker DNA, which activated the CHA reaction on the SERS probe and turned on the SERS signal of 6-carboxyl-x-rhodamine (ROX). Meanwhile, 4-MBN was used as an internal reference to calibrate the matrix interference. Thus, sensitive and reliable determination and quantification of E. coli O157:H7 was established using the ratio of the SERS signal intensities of ROX to 4-MBN. This aptasensor enabled detection of 2.44 × 10 2 CFU/mL of E. coli O157:H7 in approximately 3 h without pre-culture and DNA extraction. In addition, good reliability and excellent reproducibility were observed for the determination of E. coli O157:H7 in spiked water and milk samples. This study offered a new solution for the design of rapid, sensitive, and reliable SERS aptasensors. Graphical abstract
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BIOCHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 2.892
Ya-Jun Wang,Xiao-Peng Chen,Wei Shen,Zhi-Qiang Liu,Yu-Guo Zheng
In our previous work, we constructed a carbonyl reductase (CR) and glucose dehydrogenase (GDH) co-expression strain E. coli BL21(DE3)/pCDFDuet- gdh - cr , and a biocatalytic process of chiral diol t -butyl 6-cyano-(3 R ,5 R )-dihydroxyhexanoate ((3 R ,5 R )- 2 ) was established by using its free cells. Herein, a (3 R ,5 R )- 2 process with celite-polyethyleneimine (PEI)-glutaraldehyde (GA) immobilized E. coli BL21(DE3)/pCDFDuet- gdh - cr whole cells was developed for the first time. It was demonstrated that immobilization via cross-linking was preferred, and the optimized immobilization conditions were as follows: 0.6% (w/v) celite, 4% (w/v) PEI and 1% (w/v) GA, pH7. The immobilizate can be used at pH 7.0, 30 °C for intermittent 7 batch, yielding a total of 17.5 g (3 R ,5 R )- 2 per gram of celite-PEI-GAimmobilizates in de p (diastereomeric excess) >99.5%. Compared with the free cells, there was a dramatic improvement in celite- PEI-GA immobilizate stability and recyclability. In this way, a promising platform technology based on a combination of cells immobilization and multiple enzymes co-expression is constructed, enabling efficiently converting inexpensive prochiral ketones to high-valued chiral alcohols.
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BIORESOURCE TECHNOLOGY
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2014
·
IF 5.039
Yu-Cai He,Zhi-Cheng Tao,Xian Zhang,Zhen-Xing Yang,Jian-He Xu
An NADH-dependent reductase (CmCR) from Candida magnoliae was discovered by genome mining for carbonyl reductases. After CmCR was overexpressed in Escherichia coli BL21, a robust reductase-producing strain, recombinant E. coli CCZU-K14, was employed for the efficient synthesis of ethyl ( S )-4-chloro-3-hydroxybutanoate (( S )-CHBE) from the reduction of ethyl 4-chloro-3-oxobutanoate (COBE). After the optimization, the optimum reaction conditions were obtained. Notably, E. coli CCZU-K14 had broad substrate specificity in reducing both aliphatic and aromatic substrates, and excellent enantioselectivity of CCZU-K14 was observed for most of the tested substrates, resulting in chiral alcohols of over 99.9% ee . Moreover, COBE at a high concentration of (3000 mM) could be asymmetrically reduced to ( S )-CHBE in the high yield (>99.0%) and high enantiometric excess value (>99.9% ee ) after 14 h. Significantly, E. coli CCZU-K14 shows high potential in the industrial production of ( S )-CHBE and its derivatives (>99.9% ee ).
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Deshou Wang,Yumeng Liang,Yong Zeng,Chang Liu,Chun Zhan,Peng Chen,Shaoxian Song,Feifei Jia
The recycling of electronic waste, i.e ., waste Printed Circuit Boards (WPCBs), provides substantial environmental and economic advantages. In fact, the concentration of valuable precious and base metals in WPCBs is even higher compared to those found in mined ores. Nevertheless, it is still challenging to selectively extract precious metals with low concentrations from the pregnant leaching solution, due to the co-deposition of base metals, like Cu, which have higher concentrations. In this research, stepwise recovery of precious metals and copper directly from WPCBs thiosulfate leaching solution was facilitated by the Ti cathode coated with MoS 2 (MoS 2 /Ti). The in-situ enrichment of Au(S 2 O 3 ) 2 3- and Ag(S 2 O 3 ) 2 3- at the surface of MoS 2 enables the high efficiency and selectivity of electrodeposition , which has been confirmed through COMSOL Multiphysics simulations and visualization. As a result, the first-step electrodeposition at 0.6 V recovered 92.44 % Au and 98.18 % Ag without any co-deposition of Cu. Subsequently, the second-step recovery employed a constant current of 0.03 A, achieving 100 % recovery of copper within 12 h. Furthermore, this study optimized the reduction potential, NH 3 ·H 2 O concentration, and S 2 O 3 2- concentration for the stepwise electrodeposition process. These findings provide valuable insights for establishing a closed loop circular economy in the electronics industry.
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ANALYST
·
2024
·
IF 3.6
Zhiqi Gu,Hui Chang,Guo Yang,Boxuan Xu,Bin Miao,Jiadong Li
Vertical flow assay (VFA) is an effective point-of-care (POC) diagnostic tool for widespread applications. Nevertheless, the lack of multi-target detection and multi-signal readout capability still remain challenges. Herein, a brand new VFA scheme for multi-target saliva detection based on electronic-tags was proposed, where AlGaN/GaN HEMT sensors modified with different bio-receptors as electronic tags endowed the VFA a multi-target detection capability. In addition, the use of electronic tags instead of optical tags made that the VFA could simultaneously carry out direct multi-target readout, which ensure the effective POC diagnostic of saliva analysis. Moreover, by integrating a hydrophilically optimized micro-sieve, impurities like sticky filaments, epidermal cells and other large-scale charged particles in saliva were effectively screened, which enabled the direct detection of saliva by AlGaN/GaN HEMT sensors. Glucose, urea, and cortisol were selected to verified the feasibility of the multi-target e-VFA scheme, results displayed that the limit-of-detection (LOD) was as low as 100 aM. The linear response was demonstrated in the dynamic range of 100 aM - 100 μM, the specificity and long-term stability and the validity of the actual saliva test were also verified. These results demonstrated that the as-proposed e-VFA had potential application in saliva detection for multi-target simultaneous detection, and it is expected to achieve the real-time detection of more biological targets in saliva.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Lei Wang,Mengqing Luo,Zihao Zhang,Dan Ji,Xiaohua Chang,Yutian Zhu
Ionic conductive hydrogels as electronic skins (e-skins) have showcased pivotal potentials in the realm of human health monitoring and human–machine interfaces. However, developing intelligent hydrogel with remarkable stretchability and mechanical elasticity is yet challenging. Herein, high-performance ion-conducting hydrogels with unprecedented mechanical properties and good transparent, conductive, self-healing properties are constructed via free-radical polymerization of acrylic acid (AA) within the polyvinyl alcohol (PVA) network, and the subsequent freeze–thaw (F-T) treatment and Zr 4+ ion immersion crosslinking technique. Profiting from the synergy of multiple intermolecular/intramolecular hydrogen bonds between different polymers and coordination bonds of carboxyl-Zr 4+ , the resultant PVA/PAA/Zr 4+ hydrogel exhibits high transparency (∼97 %), superior conductivity (0.6089 S/m), a long elongation at break (2053 %), robust self-healing efficiency (96.3 %), high deformation-tolerate property, and notable mechanical repeatability. These multifaceted attributes render the hydrogel to serve as an ionic conductor for capacitive/resistive −type strain sensor. The hydrogel-based resistive-type strain sensor features a wide detection range (0.5 %-800 %), rapid response time (150 ms), long-term stability, and consistent output stability, making it promising in monitoring diverse human motions and seamless human–machine interactions. Additionally, the hydrogel sensor demonstrates excellent linear response in ultra-wide range (0–900 % strain), high sensitivity, and excellent cycling stability in a capacitive mode, enabling to be used for accurately detecting the weights of objects. As such, this work advances the development of ionic conductive hydrogel as flexible and wearable electronic devices.
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WASTE MANAGEMENT
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2025
·
IF 7.1
Ke Xu,Shengde Dong,Chunxi Hai,Luxiang Ma,Yanxia Sun,Qi Xu,Xin He,Yuan Zhou
The recycling of cathode materials in waste lithium-ion batteries is of great significance to resource utilization and environmental protection. However, few studies have addressed the challenges posed by high-aluminum LiFePO 4 black powder (HALFP-BP) resulting from inadequate pre-treatment. This study proposes a low-temperature short-time sulfuric acid leaching method based on thermodynamic analysis of E-pH diagrams. Under optimized conditions, the leaching efficiencies of Li and Fe reached 99 %, while 86 % of Al was selectively removed. Furthermore, the guiding role of E-pH diagram in the recovery process and the chemical reactions and Gibbs free energy analysis during the acid leaching process were analyzed. It revealed that the dissolution kinetics inhibition of the dense Al 2 O 3 film on the surface of aluminum impurities in the sulfuric acid system was the key to achieving selective separation. This method provides an energy-saving and efficient solution for the resource utilization of the HALFP-BP.
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Food Chemistry-X
·
2025
·
IF 8.2
Yiye Luo,Lusi Ouyang,Shuo Zhang,Yusheng Wang,Pingzhang Yao,Yin Liu,Xiaoyu Chen,Lin Yuan,Zhiyong Wu
This study investigated volatile organic compound (VOC) variations across commercial grades (G1, G2, G3) of Chinese black truffles ( Tuber indicum ) using electronic nose ( E -nose), headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPM E -GC–MS), and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). E-nose analysis revealed significant differences in sulfur-containing and terpenic compounds between grades. HS-SPME-GC–MS identified 95 VOCs, with G1 exhibiting the highest diversity (75 compounds) and unique markers like 2-butanone. Orthogonal partial least squares-discriminant analysis (OPLS-DA) highlighted 86 discriminatory VOCs (VIP > 1). Odor activity value (OAV) analysis showed G1 had the strongest aroma intensity, driven by esters (ethyl isobutyrate, OAV = 6574) and alcohols (1-octen-3-ol, OAV = 3444). Higher-grade truffles (G1) contained distinct sulfur compounds absent in lower grades. This multi-technique approach provides a biochemical basis for refining truffle grading standards beyond morphological criteria.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2021
·Vol. 23
·期 6
·1-16
·
IF 2.253
Wang Qilong,Liu Jing,Liu Jian,Thant Yaminn,Weng Wen,Wei Chunmei,Bao Rui,Adu-Frimpong Michael,Yu Qingtong,Deng Wenwen,Cao Xia,Toreniyazov Elmurat,Ji Hao,Xu Ximing,Yu Jiangnan
Recently, bisdemethoxycurcumin (BDMC), a constituent of turmeric, has been studied widely in view of their various therapeutic effects, namely anti-oxidant, anti-cancer, anti-diabetic, and anti-inflammatory. Since ancient times, turmeric has been extensively used as a naturally occurring traditional Chinese medicine (TCM) and as a crucial home remedy for treatment of many health problems such as inflammation and pain. However, the action of anti-gouty arthritis and analgesic activity of its constituents, especially BDMC, have not been scientifically reported yet. Despite the various bioactivities of BDMC, its utilization in clinics has been restricted owing to poor solubility and bioavailability. This research aimed at enhancing oral bioavailability and pharmacokinetic of BDMC via liposome formulation which was modified with d -α-tocopherol polyethylene glycol 1000 succinate (TPGS or vitamin E TPGS) and was characterized using various parameters both in vitro and in vivo. Also, the analgesic and anti-gouty activities of BDMC-loaded TPGS-modified liposomes were investigated by assessing pain threshold and serum uric acid level in potassium oxonate–induced hyperuricemic rats. As the result, BDMC-loaded TPGS-modified liposomes were formulated successfully with optimal physiochemical properties such as 75.98 ± 5.46 nm (particle size), 0.246 ± 0.011 (polydispersity index), − 38.21 ± 0.29 mV (zeta potential), 96.98 ± 0.17 (encapsulation efficiency (EE%)), and 4.84 ± 0.0089 (drug loading capacity (DL%)). Moreover, the oral bioavailability of liposomized BDMC was approximately 10-fold greater than free BDMC in vivo and in vitro cumulative release rates in pH 1.2 (78%) and pH 7 (68%) respectively. This significantly improved the sustaining drug effect of BDMC compared with free form. In addition, BDMC-TPGS liposome acted as a xanthine oxidase inhibitor to evidently reduce uric acid level in potassium oxonate–induced hyperuricemic rats and remarkably increase pain threshold capacity and reaction time in hot plate test at a dose-dependent manner. Altogether, BDMC-conjugated TPGS liposome could act as favorable nanocarriers against gouty arthritis and intense pain with prospect of overcoming the limitation of BDMC application.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2013
·
IF 2.417
Jian Xu,Yan-Yan Jia,Shao-Rui Chen,Jian-Tao Ye,Xian-Zhang Bu,Yun Hu,Yun-Zi Ma,Jin-Lei Guo,Pei-Qing Liu
Flavonoids are a class of compounds that exist in nature with the structure of 2-phenyl-chromone. In Chinese traditional medicine , herbal drugs containing flavonoids are widely used for the treatment of inflammation, cardiovascular disease, tumor and so on. In this study, we investigated the anti-inflammatory effect and related mechanisms of a novel synthetic flavonoid, (E)-1-(4-ethoxyphenyl)-3-(4-nitrophenyl)-prop-2-en-1-one (ETH) in lipopolysaccharide (LPS) stimulated macrophages. Our results showed that ETH inhibited LPS-induced TNF-α and IL-6 release in a dose-dependent manner, and decreased TNF-α, IL-1β, IL-6 and iNOS mRNA production. LPS-induced expression of cyclooxygenase-2 (COX-2) was also significantly attenuated by ETH. Pretreatment with ETH reduced the I-κBα phosphorylation, p65 nuclear translocation as well as NF-κB-dependent transcriptional activity. In addition, ETH exhibited a significant protection against LPS-induced inflammatory mortality in mice. Taken together, these findings suggest that ETH can inhibit LPS-induced inflammation via suppressing NF-κB signaling pathway , indicating that ETH may be a potential anti-inflammatory agent.
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AAPS PHARMSCITECH
·
2020
·Vol. 21
·期 5
·1-13
·
IF 2.401
Liu Dandan,Qiao Sen,Cheng Bingchao,Li Dongyang,Chen Jisuan,Wu Qingyin,Pan Hao,Pan Weisan
Despite that either non-covalent or covalent attachment of hydrophilic polymers or surfactants onto nanodiamonds (NDs) could overcome the shortcomings of being a drug delivery system, it is hard to draw a definite conclusion which strategy is more effective. Hence, with the purpose of comparing the influence of different coating approach of NDs on the oral delivery efficiency of water-insoluble model drug curcumin (CUR), NDs were firstly modified with d -α-tocopheryl polyethylene glycol 1000 succinate (TPGS) via non-covalent or covalent conjugation method, and then loaded with CUR (CUR@NDs-COOH/TPGS or CUR@NDs-TPGS). In comparison with the core-shell-structured CUR@NDs-COOH/TPGS, CUR@NDs-TPGS were irregular in shape with dense TPGS film, and exhibited smaller size, more negatively potential, and higher drug loading efficiency. The covalent connection group also showed higher anti-cancer activity, cellular uptake, and permeability through the Caco-2 cell monolayers, as well as favorable distribution, penetration, and retention in rat intestines. The oral bioavailability study in rats demonstrated that CUR@NDs-TPGS showed significantly greater C max and AUC 0− t in contrast with CUR suspension and the TPGS-coated ones, respectively. The findings illustrated that covalent grafting TPGS onto the surface of NDs possesses better efficacy and biocompatibility on oral delivery of poorly soluble drug CUR than pristine and non-covalent coated nanoparticles.
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CATALYSIS LETTERS
·
2021
·Vol. 151
·期 9
·2527-2536
·
IF 3.186
Du Hui-Jun,Luo Wei,Appiah Bright,Zou Zhi-Cheng,Yang Zhong-Hua,Zeng Rong,Luo Li
Abstract The intracellular NADPH insufficient supply is the main bottleneck to the synthesis of chiral alcohols by asymmetric reduction with whole-cell catalysis. Herein, we provide a novel strategy to strengthen intracellular NADPH supply through introducing an NADP + -dependent glyceraldehyde 3-phosphate dehydrogenase ( gap B from Bacillus subtilis 168) into the Embden-Meyerhof pathway and a NAD kinase ( yfj B from E. coli MG1655) to further enhance the NADP(H) pool. A recombinant E. coli ( E. coli BL21 (DE3)/pETDuet-1- gap B- yue D&pET28a- yfj B) was constructed to co-express gap B and yfj B with a carbonyl reductase gene yue D together. The result showed that the intracellular NADPH amount increased by 134.4% with the strategy. To the model reaction (asymmetric reduction of acetophenone to S -phenyl ethanol), the yield was 3.7-fold with this strategy compared to the control. This provides a technological route for strengthening the intracellular NADPH supply in E. coli for biocatalysis and biosynthesis. Graphic Abstract
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