Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 10
·1688-1694
·
IF 5.068
Lai Yuxuan,Deng Yan,Yang Gaojian,Li Song,Zhang Chuanxiang,Liu Xueying
A novel molecular imprinted polymer (MIP) electrochemical sensor was successfully fabricated for sensitive detection of carcinomaembryonic antigen (CEA). We used CEA as template, dopamine (DA) as imprinted monomers. Through controlling electropolymerization, a "PDA-CEA"complex was achieved.
After elution, the specific cavities adsorbed the target molecules. In addition, polythionine (PTh) and AuNPs were applied as the electrode modifying materials to enhance electron transfer rate and improve detection signal. Using differential pulse voltammetry (DPV) detection, the peak current
decreased with the increase in concentration of CEA, and the linear response range of the MIP sensor was from 0.001 ng/mL to 1000 ng/mL with the detection limit as low as 0.2589 pg/mL. The MIP sensor had a low sample consumption, good stability, and high sensitivity, and could become a new
promising method for the detection of CEA. Furthermore, this MIP sensor was demonstrated in testing CEA in human serum sample with satisfactory results.
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高影响因子
BIOELECTROCHEMISTRY
·
2018
·
IF 3.789
Baoping Lu,Xuna Yuan,Yuehong Ren,Qinghua Shi,Song Wang,Jinlong Dong,Ze-dong Nan
We describe a facile method to synthesize a new type of catalyst by electrodepositing Ag nanocrystals (AgNCs) on the different polymer dyes, Poly (methylene blue) (PMB) or Poly (4-(2-Pyridylazo)-Resorcinol) (PAR) modified graphene‑carbon spheres (GS) hybrids. The self-assembled GS take dual advantages of carbon spheres and graphene. Carbon spheres acts as nano-spacers prevent the aggregation of graphene and guarantee the fast electron transfer of GS. Secondly, polymerized dyes used here are beneficial for AgNCs growing as a linker. The effects of dyes on the growth habits, morphologies and catalytic properties for AgNCs were investigated. A novel electrochemical nonenzymatic sensor for hydrogen peroxide (H 2 O 2 ) detection is fabricated based on the Ag/Polymer dyes/GS ternary composites modified glass carbon electrode (GCE) for the first time. It was found that the proposed electrodes, especially for Ag/PMB/GS/GCE, displayed a peculiar electrocatalytic activity towards H 2 O 2 reduction synergistically as compared to Ag/PAR/GS/GCE or Ag/GS/GCE alone. Ag/PMB/GS/GCE showed a linear response over the H 2 O 2 concentration range of 0.5 to 1112 μM. The detection limit and sensitivity is 0.15 μM and 400 μA mM −1 cm −2 , respectively. These outstanding results enable the practical application of Ag/PMB/GS/GCE for the H 2 O 2 tracking released from MCF-7 (human breast cancer cells) with satisfactory results.
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ELECTROCHIMICA ACTA
·
2018
·
IF 5.116
Fangming Lou,Anjun Wang,Jing Jin,Qunfang Li,Shenghui Zhang
We report here one-pot green synthetic method for preparing popcorn-like Au@Polyluminol nanoflowers by using the HAuCl 4 to directly oxidize luminol at a specific controlled mass ratio of HAuCl 4 : luminol in hydrothermal conditions for the first time. The Au@Polyluminol nanoflowers were attached to the surface of a GCE electrode to form a highly solid-state electrochemiluminescent (ECL) sensor, which exhibited strong and stable ECL intensity, showed excellent ECL behavior. Further, the as-prepared Au@Polyluminol nanoflowers could also be used for the immobilization of uricase on an electrode surface to fabricate a sensitive solid-state Uric Acid ECL biosensor, which indicate that the Au@Polyluminol nanoflowers is a promising candidate for constructing various sensitive solid-state ECL sensors and promoting the application in electroanalytical and biochemical analysis.
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RSC Advances
·
2018
·Vol. 8
·期 29
·15906-15914
·
IF 2.936
Zhaokun Yan,Qingtang Wang,Xiaolong Liu,Jun Peng,Qin Li,Ming Wu,Jiumao Lin
Ursolic acid (UA) has shown great potential in cancer therapy but their efficacy is seriously compromised by poor water-solubility and limited cellular uptake. In this paper, cationic nanomicelles self-assembled from Pluronic F127 with the cationic polymer of C18-polyethylenimine (C18-PEI) as a functional component are fabricated as delivery vehicles of Chinese herbal medicine active components of ursolic acid (UA) for colorectal cancer treatment. The inhibition effects of this drug loaded cationic nanomicelles (named as FUP) on cell viability and cell colony formation were more significant than the free UA, due to their cationic surface which can increase UA uptake by colorectal cancer cells. Cell cycle analysis showed that this inhibition effect was mediated by a cell cycle arrest at the G1 checkpoint, and the cell death induced by these nanomicelles occurred via apoptosis, which was detected by annexin V antibody and propidium iodide staining. Further western blot analysis demonstrated the apoptosis mechanism was associated with the regulation of Fas/FasL and activation of caspase-8 and caspase-3. Therefore, our cationic nanomicelles can potentially be used to enhance the therapeutic effect of UA for colorectal cancer treatment.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Weiwei Mao,Haiping He,Zhizhen Ye,Jingyun Huang
A facile and in-situ electrochemical nanofabrication towards three-dimensional graphene@nickel foam (3DGF) to produce Ni(OH) 2 nanosheets was proposed and the integrated Ni(OH) 2 /3DGF electrode for glucose sensing was researched. The nanofabrication of chemical battery reaction is mild and facile. Nanopores formation plays a key role in promoting the growth of Ni(OH) 2 nanosheets, leading to enhanced electro-catalysis. In this constructed unique 3D hierarchical architecture, graphene layers not only provide excellent electrical conductivity but also benefit to the sensing stability, while the 3D interconnected structure prevents the agglomeration or exfoliation of Ni(OH) 2 nanosheets and the porous Ni(OH) 2 film benefits to the mass and charge transfer, and thus improving the sensing performance remarkably. As a successful demonstration, the fabricated Ni(OH) 2 /3DGF glucose sensor exhibits an appreciable sensitivity 2366 μA mM −1 cm −2 and a low detection limit of 0.32 μM. This study proposes an easy and convenient strategy to prepare Ni(OH) 2 and graphene integrated glucose sensor, promisingly applied in practice.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Qiuxuan Yang,Qilong Wang,Wenwen Deng,Congyong Sun,Qiuyu Wei,Michael Adu-Frimpong,Jixiang Shi,Jiangnan Yu,Ximing Xu
In this present study, we investigated the anti-hyperuricemic and anti-gouty arthritis effect of a puried water-soluble polysaccharide (LJP-1) obtained from Lonicera japonica . A series of characterization of the purified polysaccharide were carried out in this paper. Monosaccharide analysis showed that LJP-1 composed of glucuronic acid, glucose, galactose, arabinose, and xylose at the ratio of 2.43:1:2.09:1.95:1.96, respectively. The estimated molecular weight of LJP-1 was 17.5 kDa. LJP-1 belonged to pyranose and possessed α- and β -glycosidic configurations. Congo red test showed that LJP-1 had a spatial triple helix structure. In pharmacodynamic experiments, the anti-hyperuricemic activity of LJP-1 was studied using hyperuricemic SD rat model induced via potassium oxonate and hypoxanthine. The result showed that LJP-1 could obviously decrease the serum uric acid level and suppress xanthine oxidase (XOD) activity. Moreover, in the gouty arthritis model established by sodium urate crystals, the degree of swelling of the ankle joint, IL-1β, IL-6, TNF-α and COX-2-related inflammatory factors levels in murine serum all declined. Taken together, these results demonstrated that LJP-1 has anti-gouty arthritis effect. Therefore, LJP-1 could serve as a promising candidate for developing novel natural anti-gouty agent.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jingling Wu,Peiyao Ran,Shu Zhu,Fangjing Mo,Cun Wang,Yingzi Fu
We present a highly sensitive platinum nanoparticles (PtNPs), rhombus-shaped rubrene microsheets (RubRMs) and dissolved oxygen (O 2 ) system for the monitoring of l -cysteine by the quenched electrochemiluminescence (ECL) signals. Firstly, RubRMs has been prepared through reprecipitation method, then the metal organic composites of PtNPs-RubRMs was synthesized by a simple in situ reduction of PtNPs on RubRMs surface. The composites combined the high ECL characteristic of RubRMs, the wonderful catalytic efficiency and good electron transfer ability of PtNPs. With the synergistic effect between RubRMs and PtNPs, the proposed ECL sensors exhibited excellent responses for l -cysteine. Under the optimum conditions, the linear range was from 1.0 × 10 −9 mol L -1 to 5.0 × 10 -4 mol L -1 with a detection limit of 3.3 × 10 -10 mol L -1 (S/N = 3). And the results shows high sensitivity, excellent stability, and low background signal. It is simple to prepare and operate for the practical application.
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Molecular Medicine Reports
·
2018
·Vol. 18
·期 3
·3193-3202
·
IF 1.922
Bo Yan,Dongsheng Wang,Qiang Wang,Xiaolan Lu,Qin Du,Qi Liang,Xingliang Jiang,Xiaolan Guo,Jingguo Zhou,Yan Xing
At present, the majority of methods used for uric acid (UA) detection are not able to meet the detection requirements with speed, accuracy, high sensitivity, high specificity, a wide linear range or a low cost. Compared with other methods, the electrochemical method has a high sensitivity and fast detection. The present study aimed to identify an electrochemical sensor with high sensitivity, fast detection and a wide linear range for the detection of UA. A glassy carbon electrode modified with graphene‑molybdenum disulfide‑Nafion (G‑MoS2‑Nafion) composites was prepared for use as the working electrode. The morphologies and elemental compositions of the G‑MoS2 composites were characterized by field emission scanning electron microscopy, elemental distribution spectrometry and X‑ray diffraction, respectively. The electrochemical behaviors were investigated by cyclic voltammetry, linear sweep voltammetry and the amperometric i‑t curve (i‑t). The interference of glucose, ascorbic acid and dopamine, and the accuracy and precision of the electrochemical method were subsequently evaluated. The present study identified the following: (1) Only the reduction peak of UA was detected in human serum, indicating that the method established in the present study has a high specificity for the determination of UA in human serum; (2) UA concentration has a linear correlation with current intensity (y=0.012x+0.998; R2=0.998), wide linear range and high sensitivity (minimum detectability=13.91 µM; signal‑to‑noise ratio=3); (3) the values of UA content in human serum were positively proportional to the clinical results (y=0.9802x+11.494; R2=0.978); (4) the average recovery rate of UA (95.28%) and the replicability assay of the i‑t electrochemical method (coefficient of variation=2.04%), suggest that the method had a high accuracy and good precision for UA detection. Due to its characteristics of good accuracy, high sensitivity, wide linear range, good anti‑interference ability and replicability, G‑MoS2‑Nafion has good prospects for UA detection in the clinical setting.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 29
·7663-7670
·
IF 3.307
Liu Fuyan,Wang Peng,Zhang Qianqian,Wang Zeyan,Liu Yuanyuan,Zheng Zhaoke,Qin Xiaoyan,Zhang Xiaoyang,Dai Ying,Li Lu,Huang Baibiao
Novel porous Co 3 O 4 nanosheets (NSs) were synthesized on the flexible carbon cloth (CC) substrate by a facile hydrothermal method and applied to construct a non-enzymatic sensor for glucose detection. The sensor is based on the electro-catalytic oxidation of glucose on the surface of Co 3 O 4 NSs. Since this particular nanostructure can provide large surface area and more active sites, the Co 3 O 4 NSs non-enzymatic sensor exhibits excellent analytical performance, such as a high sensitivity (8506 μA mM −1 cm −2 ), a fast response time (less than 6 s), low detection limit of 1 μM, good selectivity, and long-term stability. The results suggest that the porous Co 3 O 4 NSs have great potential applications in the development of sensors for enzyme-free detection of glucose.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-12
·
IF 5.705
Xu Cuxing,Hei Yashuang,Liu Jingju,Sun Mimi,Sha Tianze,Wang Nan,Hassan Mehboob,Bo Xiangjie,Zhou Ming
The authors describe a method for synthesis of a three-dimensional (3D) interconnected carbon nanorod aerogel (3D-ICNA) starting from wax gourd ( Benincasa hispida ) which is a low-cost biomass. The 3D-ICNA possesses unique 3D interconnected and porous nanostructure, with abundant edge-plane-like defective sites, a large specific surface area (823 m 2 g −1 ) and a large pore volume (0.12 cm 3 g −1 ). This makes the material attractive in terms of electrochemical sensing. To validate the feasibility, the voltammetric response towards ferricyanide, hydrogen peroxide (H 2 O 2 ), acetaminophen, ascorbic acid (AA), dopamine, uric acid and epinephrine was investigated by using a glassy carbon electrode (GCE) modified with 3D-ICNA. The modified GCE shows higher electron-transfer capacity than a conventional GCE. In addition, as an electrochemical sensor for AA or H 2 O 2 , the electrode exhibits better analytical performance with lower detection limit [3.5 μM for AA or 0.68 μM for H 2 O 2 based on 3σ/m criterion (where σ is the standard deviation of the blank and m is the slope of the calibration plot)], wider linear range and higher sensitivity (0.14, 0.11 and 0.080 μA μM −1 cm −2 for AA or 0.24 and 0.20 μA μM −1 cm −2 for H 2 O 2 ) compared to a plain GCE or a carbon nanotube-modified GCE. The modified GCE exhibits a large potential for the amperometric determination of AA or H 2 O 2 in real samples.
By employing the biomass of wax gourd ( Benincasa hispida ) as the precursor, a three-dimensional interconnected carbon nanorod aerogel was prepared. It is shown to be a viable material for the construction of an advanced electrochemical sensor for H 2 O 2 and ascorbic acid.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Lanfang Wang,Weiqi Zhu,Wenbo Lu,Lina Shi,Rui Wang,Ruixue Pang,YueYue Cao,Fang Wang,Xiaohong Xu
A novel nonsemiconductor photoelectrochemical biosensor was first constructed using the unique plasmonic AuNi nanodendrite arrays. The AuNi nanodendrite arrays were rapidly prepared by a one-step electrodeposition method using the porous anodic aluminum templates. Owing to its hierarchical structure with abundant active sites, the synergistic catalytic of Au and Ni can be better exploited. These plasmonic AuNi nanodendrite arrays display exceptional photoelectrocatalytic activities for glucose oxidation and hydrogen peroxide reduction reaction under visible light illumination. Specifically, the detection sensitivity for glucose (3.7277 mA mM −1 cm −2 ) under illumination is about 3.3 folds improvement than in the dark (1.1287 mA mM −1 cm −2 ), together with high accuracy and low detection limit of 3 μM. The markedly enhanced performance of AuNi nanodendrite arrays can be attributed to its hierarchical structure with abundant active sites and plasmonic effect of Au with strong absorption band in visible region. Such a newly developed method via the facile and low-cost route is of great significance in designing the plasmon-aided photoelectrochemical biosensors.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-8
·
IF 5.479
Zhou Jiao,Ling Yu,Li Nian Bing,Luo Hong Qun
The authors describe a fluorometric and resonance Rayleigh scattering dual-mode scheme for detection of the activity and inhibition of alkaline phosphatase (ALP). The method utilizes (a) CoOOH nanoflakes, which have high resonance Rayleigh scattering activity and can strongly adsorb ssDNA, and (b) Co(II)-dependent DNAzyme assisted signal amplification. ALP specifically catalyzes the hydrolysis of ascorbic acid-2-phosphate to produce ascorbic acid which reduces CoOOH nanoflakes to Co(II) ion. The Co(II)-dependent DNAzyme is then activated by Co(II) ion, and this results in the cleavage of a substrate labeled with both a fluorophore and a quencher. Following hydrolysis, fluorophore and quencher become separated and the fluorescence measured at excitation/emission wavelengths of 490/518 nm recovers, while the RRS signal at 405 nm decreases. The method works on the 0.2 to 2000 U L −1 ALP activity range, and the detection limit is 0.05 U L −1 . The method was used to validate the mechanism of the action of two classical ALP inhibitors (EDTA and Na 3 VO 4 ). Conceivably, it can also be applied to screen for ALP inhibitors.
A bioprobe composed of cobalt oxyhydroxide (CoOOH) nanoflakes as the substrate and carrier, and Co 2+ -dependent DNAzyme containing guanine ribonucleotide (rG) and uracil ribonucleotide (rU) for circularly signal amplification is used for fluorometric determination of alkaline phosphatase (ALP) activity.
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高影响因子
危险品化学品经营许可证（带存储）