INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Honghong Deng,Xi Liang,Ziyang Zhang,Chao Peng,Xueying Wang,Liumin Li,Binqi Li,Qiang Yang,Junwei Liu,Muhammad Moaaz Ali,Sheng-Yen Wu,Faxing Chen
Plums are economically important stone fruits, valued for their nutritional quality and health-promoting properties. However, their quality and marketability are often compromised by a physiological disorder called fruit hollowness and browning (HB). This study employed an integrated physiological, biochemical, and proteomic approach to investigate the molecular mechanisms underlying HB in Huangguan plum. Huangguan plum fruit samples from seven developmental stages were analyzed for lignin content, key enzyme activities, and expression patterns of genes involved in lignin biosynthesis. The results demonstrated that HB-affected fruit accumulated significantly higher lignin levels, particularly during post-veraison stages, accompanied by elevated activities of lignin biosynthesis-related enzymes like phenylalanine ammonia-lyase and laccase. Expression analysis of lignin pathway genes revealed distinct transcriptional dynamics, with notable upregulation of PsPAL1 and PsLAC7 in HB fruit at critical developmental stages. Proteomic profiling identified 941 and 1249 differentially expressed proteins between HB and non-HB fruit at the veraison and ripe stages, respectively. KEGG enrichment analysis highlighted significant alterations in the phenylpropanoid biosynthesis pathway (map00940), which is central to lignification. These findings provide new insights into the physiological and molecular basis of HB in plum fruit and a scientific foundation for developing effective strategies to mitigate or address this disorder and improve fruit quality.
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高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Xishun Peng,Zhongchen Bai,Caizhen Zu,Yuxue He,Qi Zhao
The presence of thiram residues on fruit peels posed a significant risk to human health. Here, we prepared an electrochemical surface-enhanced Raman spectroscopy (EC-SERS) sensor of SPE/RGO/Au@Ag flexible film to detect thiram residues on fruit peel. The surface of this film was evenly covered with Au@Ag nanoparticles, which allowed it to specifically identify thiram residues through the formation of Ag-S bond. In addition, an electrochemical method was used to modulate the energy level matching between the SERS film and the thiram molecules to improve the chemical enhancement effect of the SERS. The results indicated that the charge transfer degree between the SERS film and the thiram molecules was up to 0.875. The SPE/RGO/Au@Ag film achieved a limit of detection (LOD) of 2.1 pM at -1.3 V. Owing to its excellent SERS performance, the SPE/RGO/Au@Ag film could successfully recognize the thiram residues on apple and orange peels, with recoveries of 91% to 107% and from 89% to 102%, respectively. The flexible EC-SERS sensor offers considerable promise for sensitively recognizing thiram residues on fruit peels.
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Nutrients
·
2025
·Vol. 17
·期 21
·3463
·
IF 5
Yang Liu,Jingzhong Zheng,Xin Zheng,Dan Zhou,Hang Ma,Xue Zhou,Fahuan Ge
Background:Cili (Rosa roxburghiiTratt) is a unique fruit native to China’s Yunnan–Guizhou Plateau, rich in vitamin C, polyphenols, and triterpene, with broad health-promoting effects. Although cili’s hepatoprotective properties are reported, the bioactive components and underlying mechanisms remain poorly defined.Methods:We enriched proanthocyanidins from cili using column chromatography, identified their components via UPLC-Q-TOF-MS/MS, and validated their anti-liver fibrosis effects through in vitro and in vivo experiments.Results:Herein, we developed a novel proanthocyanidin-rich cili fruit extract (PACs-CFE) containing 84.2% total proanthocyanidins, comprising catechins, epicatechins, and diverse B-type dimers, trimers, tetramers, and gallate esters, as characterized by UPLC-Q-TOF-MS/MS. PACs-CFE inhibited LX-2 activation, suppressed collagen III and α-SMA expression, and induced ferroptosis via mitochondrial injury, reactive oxygen species accumulation, and GPX4/ferritin downregulation. In vivo, PACs-CFE ameliorated liver fibrosis, restored hepatic architecture, and improved serum alanine aminotransferase, aspartate aminotransferase, and bilirubin profiles. Moreover, PACs-CFE modulated the TGF-β1/Smad3 signaling pathway and beneficially reshaped the gut microbiota, enriching anti-inflammatory and hepatoprotective genera while reducing pathogenic taxa.Conclusions:Our findings show that PACs-CFE exerts multi-targeted anti-fibrotic effects through hepatic stellate cell inactivation, ferroptosis induction, TGF-β1/Smad3 suppression, and gut–liver axis modulation. This study provides useful insight into the hepatoprotective potential of cili fruit and supports its development as standardized functional ingredients for liver health.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Jing Chen,Yuan Ma,Zhi-Min Rao,Song-Lin Jiang,Ying-Jun Lou,Karim Malik,Arman Chowdhury,Hua-Zhong Ying,Chen-Huan Yu
The development of potent adjuvants is crucial for enhancing influenza mRNA vaccine efficacy. Tremella aurantia polysaccharides possess bioactivities, but their structural complexity and adjuvant potential remain unclear. In this study, a high-molecular-weight (Mw: 1178.77 kDa) polysaccharide (TAP) was purified from T. aurantia fruit bodies and hydrolyzed by using an ultrasonic-assisted H 2 O 2 -Cu 2+ method, yielding three products (designated TAP1-TAP3). Structural analyses revealed that TAP1 (426.28 kDa) and TAP2 (16.96 kDa) retained the native monosaccharide composition (Man, GlcA, Xyl, Fuc), whereas TAP3 (21.97 kDa) lost its fucose residues. All polysaccharides shared a 1,3-α-D-mannose backbone with GlcA and Xyl side chains. TAP2, a novel low-Mw fucosylated glucuronoxylomannan, exhibited superior immunostimulatory activity in vitro with minimal cytotoxicity. When used as an adjuvant with a seasonal influenza HA mRNA vaccine in mice, TAP2 significantly enhanced hemagglutination inhibition titers, increased influenza-specific IgG levels, and improved survival rates against a lethal influenza virus challenge. Mechanistically, the immunoenhancing effect of TAP2 was attributed to its optimal molecular size and sulfated fucose branches, which potently promoted dendritic cell maturation primarily via activation of the TLR4/MyD88/NF-κB pathway. This study not only identified TAP2 as a promising adjuvant candidate for mRNA vaccines but also elucidated the structure-activity relationship of Tremella glucuronoxylomannans.
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Food & Function
·
2015
·Vol. 6
·期 12
·3693-3701
·
IF 2.791
Qian Li,Jun Chen,Ti Li,Chengmei Liu,Yuxin Zhai,David Julian McClements,Jiyan Liu
Bioactive proanthocyanidins were isolated from the peel of Choerospondias axillaris fruit, which is a waste product of the food processing industry. Compositional analysis indicated that the proanthocyanidins had extension units mainly consisting of epicatechin gallate or epicatechin, and terminal units mainly consisting of catechin. Numerous polymeric forms of the molecules were detected, including monomers, dimers, and trimers. Certain fractions exhibited strong α-amylase or α-glucosidase inhibition in a dose-dependent manner. Furthermore, their inhibitory activities depended on their degree of polymerization and galloylation. For example, the most bioactive fraction had α-amylase and α-glucosidase inhibitory activities (IC50 values) of 541 and 3.1 μg mL−1, respectively. This study demonstrates that proanthocyanidins from C. axillaris peels can inhibit carbohydrate digestive enzymes in vitro and may therefore serve as antidiabetic ingredients in functional or medical foods.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Mingxiu Long,Hui Wang,Fang Zhang,Yaoqi Ran,Yongfu Li,Jingwen Luo,Zhaojun Chen,Zhuxi Tian,Shuming Tan,Xiong Liu
Rosa roxburghii Tratt fruit polyphenols (RP) possess antioxidant, anti-inflammatory, and immunomodulatory properties, showing potential for ulcerative colitis (UC) treatment. However, their efficacy is limited by bioavailability and targeting efficiency. To address this, RP were encapsulated into acylated chitosan-crosslinked carboxymethyl konjac glucomannan (ACs-CKGM) nanogel (RPNG). Experimental results demonstrated that RPNG significantly enhanced intestinal barrier function by upregulating tight junction proteins (ZO-1, Occludin and Claudin-3) and mucus secretion (MUC2). It effectively mitigated oxidative stress by restoring antioxidant enzymes (SOD, GSH-Px and CAT) while reducing MPO, COX-2 and iNOS activation. Through integrated ELISA, western blot, and transcriptomic analyses, we emonstrated that RPNG specifically inhibited PI3K/Akt/mTOR and NF-κB signaling pathways, decreasing inflammatory cytokines (e.g., TNF-α, IL-6) while increasing anti-inflammatory mediators (e.g., IL-10). Gut microbiome analysis showed RPNG reversed DSS-induced dysbiosis by enriching beneficial bacteria (e.g., Muribaculum sp.) and suppressing pathogens (e.g., Escherichia coli ). These findings highlight RPNG as a novel targeted strategy for UC management, supported by multi-omics mechanistic insights.
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Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Yuan Jiangjuan,Huang Xinyi,Pei Dong,Wang Han,Liu Yun,Ren Xiangping,Zhao Lichun,Kan Huan,Qu Qingli
Olea europaea L. (OEL) fruits play a significant role in the Mediterranean diet. OEL fruits contain abundant bioactive components and have been reported to possess potential antioxidant and xanthine oxidase (XOD) inhibition effects. However, the active components for XOD inhibition effects and related mechanisms are still unclear. In this study, the antioxidant activity of OEL fruits was assessed and showed a good effect on antioxidants. After that, the XOD inhibition effects of OEL fruits were investigated. The results indicated that OEL fruits also have a good impact on inhibiting XOD, with IC 50 values of 208.2 ± 1.58 μg/mL. Under the optimal experimental conditions, six potential XOD ligands in OEL fruits were screened out and three phenylethanol analogues were identified (hydroxytyrosol, 3,4-dihydroxy phenyl ethyl acetate, oleuropein). Compared to the inactivated group, 3,4-dihydroxy phenyl ethyl acetate had the highest affinity, indicating it might be the main component of the OEL fruits that inhibit XOD. Molecular docking results elucidated that the potential active components mainly form hydrogen bonds with XOD. The results showed that OEL fruits possessed potential antioxidant and XOD inhibitory effects and that the affinity ultrafiltration (UF) method is an effective method to screen potential ligands for XOD from natural products.
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JOURNAL OF EXPERIMENTAL BOTANY
·
2018
·Vol. 69
·期 10
·2595-2608
·
IF 5.354
Luo Huifeng,Dai Cheng,Li Yongping,Feng Jia,Liu Zhongchi,Kang Chunying
The red color of the foliage and fruit in strawberry comes from anthocyanins stored in the vacuole; however, how this anthocyanin accumulation is regulated remains unclear. A reduced anthocyanin in petioles (rap) mutant was identified in an N-ethyl-N-nitrosourea (ENU) mutagenized population of YW5AF7, a white-fruited variety of the wild strawberry Fragaria vesca. The causative mutation was identified to be a premature stop codon in a glutathione S-transferase (GST) gene. In addition to the foliage coloration, RAP also mediates fruit pigmentation and acts downstream of the fruit-specific transcription factor FvMYB10. Among all eight GST genes in the same subfamily, RAP is most abundantly expressed in the ripening fruit. Expression analysis and transient expression assays demonstrated that RAP is the principal transporter of anthocyanins among the paralogs. Moreover, domain-swap experiments showed that both the N- and C-terminals of RAP are essential for the binding capability of anthocyanins. In addition, transient knock-down of RAP resulted in reduced fruit coloration in cultivated strawberry. Collectively, our results demonstrate that RAP encodes the principal GST transporter of anthocyanins in the strawberry foliage and fruit, and it could be modified to alter the fruit color in strawberry.
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Food Analytical Methods
·
2019
·Vol. 12
·期 12
·2777-2784
·
IF 2.413
Li Hong,Zhao Chunxi,Tian Hao,Yang Yaling,Li Wanyi
An uncomplicated, valid, and convenient way for detection of lycopene and β-carotene in fruit juice samples using acid–base-induced deep eutectic solvent (DES) liquid-liquid microextraction (DES-ABLLME) prior to HPLC was developed. The ternary fatty acid DESs were prepared by C 9 :C 10 :C 11 (2:1:1), which serve as hydrogen bond donors and hydrogen bond acceptors synchronously. In extraction procedure, NH 3 ·H 2 O was used as the emulsifier and reacted with DESs with a milk solution, which can significantly increase the extraction efficiency. HCl was used as the de-emulsifier, which could break the state of emulsification. At optimum conditions, the calibration graphs were linear in the concentration range from 0.1 to 100 μg mL −1 for lycopene and from 0.025 to 5.00 μg mL −1 for β-carotene. The limits of detection (LODs) were 0.05 and 0.002 μg mL −1 for lycopene and β-carotene, respectively. The RSD values were varied from 1.3 to 4.1%. Finally, the proposed method was applied to analyze β-carotene and lycopene in fruit juices.
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JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
·
2019
·Vol. 144
·期 6
·420-428
·
IF 0.829
Yu Wang,Haobo Yang,Shuai Zhong,Xin Liu,Tong Li,Chengwen Zong
Sugar and organic acid contents as well as sugar-to-acid ratio of fruit are important factors affecting fruit quality and processing. In this study, we compared sugar and organic acid contents of Vaccinium uliginosum (bog bilberry) fruit harvested from 100 plants across 10 wild populations growing in the Changbai Mountains of China. Correlation of sugar and acid contents with the spatial distribution of these populations was accessed. Germplasm with high sugar and low acid with potential for use in future breeding was also identified. Results showed that sugar and organic acid contents varied among different V. uliginosum populations in the Changbai Mountains. Fructose (18.60–38.54 mg·g−1) and glucose (15.77–35.08 mg·g−1) were the main sugars, whereas quinic (16.06–20.97 mg·g−1), citric (10.39–14.96 mg·g−1), and malic (4.28–7.47 mg·g−1) acids were the main organic acids in V. uliginosum. The first population collected in the Dongfanghong forest farm (lat. 42°00′N, long. 128°03′E) showed the highest average sugar content and sugar-to-acid ratio among the sampled populations. Moreover, total sugar content and sugar-to-acid ratio of Sample 7—Population 1, which is located at Manjiang forest farm (lat. 41°09′N, long. 127°05′E) was much higher than the other 99 samples, making this sample representative of important germplasm for breeding. Altitude and latitude were important environmental factors affecting sugar and organic acid contents. Fructose, glucose, and total sugar contents were positively correlated with altitude and negatively correlated with latitude. Oxalic acid content and sugar-to-acid ratio were positively correlated with altitude, and there was no significant correlation between organic acid contents and latitude.
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PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2025
·
IF 3.3
Houyin Zhou,Kun Guo,Jingzhi Yang,Gul Umair,Xianhui Yin,Yue Ma,Jun Yuan
The Asian plum ( Prunus salicina ) is widely cultivated in China for its nutritional and economic importance. In 2024, a previously unreported fruit spot disease was observed on plums in Guizhou Province, China; affected fruit developed depressed lesions that progressed to rot, resulting in substantial economic losses. The causal agent was identified as Fusarium oxysporum based on morphology and a multilocus phylogenetic analysis of TEF1-α , RPB2 , and TUB2 . Pathogenicity assays that fulfilled Koch's postulates confirmed F. oxysporum as the etiological agent of the disease. Fungicide sensitivity of the F. oxysporum isolate was evaluated using the mycelial growth rate method across 13 active ingredients, and field efficacy trials were conducted with six fungicides. Prochloraz (45.0 % EW), tebuconazole (43.0 % SC), and azoxystrobin (25.0 % SC) strongly inhibited mycelial growth, with EC 50 values of 0.0570, 0.0810, and 0.0592 μg mL, respectively. In field trials, prochloraz (45.0 % EW) and tebuconazole (43.0 % SC) provided excellent control of plum fruit spot, with control efficacies of 80.31 % and 78.39 % at 7 days and 76.55 % and 73.50 % at 14 days after the final application, respectively. To the present knowledge, this is the first report of F. oxysporum causing fruit spot on plum in China, and the results identify effective chemical options for disease management.
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MOLECULES
·
2024
·Vol. 29
·期 8
·1733
·
IF 4.2
Shuo Zhang,Ze Liu,Xinyu Xu,Ruihua Zhao,Shujiang Zhang,Rong Luo
In traditional Chinese medicine, Aurantii Fructus Immatures (AFIs) have been utilized for more than 2000 years. The proportions of different fruit parts are crucial for evaluating AFI quality in China. However, the basis for this statement’s substance is unclear. Differences in quality are intimately correlated with a plant’s metabolite composition. On the basis of a widely targeted metabolome, this study intended to investigate the metabolite composition and evaluate the antioxidant capacity of the peel and pulp of an AFI. Metabolites were identified and quantified by UHPLC-QqQ-MS. To assess their antioxidant ability, DPPH and ABTS assays were carried out. There were 1327 chemical compounds identified by UHPLC-QqQ-MS. After screening the differential metabolites using a multivariate statistical analysis, it was found that there were 695 significant differences in the metabolites between the peel and the pulp. Among them, it was discovered that the content of active ingredients in the peel group was higher than that in the pulp group. Furthermore, the aqueous extracts from the peel showed stronger antioxidant capacities than those from the pulp. The metabolites and antioxidant capacities were significantly different between the peel and the pulp. This study of different fruit parts might provide a guide for AFI quality assessments.
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高影响因子
危险品化学品经营许可证（带存储）