ARCHIVES OF TOXICOLOGY
·
2021
·Vol. 95
·期 8
·2839-2850
·
IF 5.153
Wan Jingjing,Chen Ruixue,Yang Zhou,Xi Jing,Cao Yiyi,Chen Yu,Zhang Xinyu,Luan Yang
Aristolochic acids (AAs) are a family of natural compounds with AA I and AA II being known carcinogens, whose bioactivation causes DNA adducts formation. However, other congeners have rarely been investigated. This study aimed to investigate genotoxicity of AA IVa, which differs from AA I by a hydroxyl group, abundant in Aristolochiaceae plants. AA IVa reacted with 2'-deoxyadenosine (dA) and 2'-deoxyguanosine (dG) to form three dA and five dG adducts as identified by high-resolution mass spectrometry, among which two dA and three dG adducts were detected in reactions of AA IVa with calf thymus DNA (CT DNA). However, no DNA adducts were detected in the kidney, liver, and forestomach of orally dosed mice at 40 mg/kg/day for 2 days, and bone marrow micronucleus assay also yielded negative results. Pharmacokinetic analyses of metabolites in plasma indicated that AA IVa was mainly O -demethylated to produce a metabolite with two hydroxyl groups, probably facilitating its excretion. Meanwhile, no reduced metabolites were detected. The competitive reaction of AA I and AA IVa with CT DNA, with adducts levels varying with pH of reaction revealed that AA IVa was significantly less reactive than AA I, probably by hydroxyl deprotonation of AA IVa, which was explained by theoretical calculations for reaction barriers, energy levels of the molecular orbits, and charges at the reaction sites. In brief, although it could form DNA adducts in vitro, AA IVa was non-genotoxic in vivo, which was attributed to its low reactivity and biotransformation into an easily excreted metabolite rather than bioactivation.
研究领域:
药理学
·
毒理学
·
分子生物学
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DOI
PubMed
高影响因子
Journal of Saudi Chemical Society
·
2021
·
IF 3.932
Smaher M. Elbayomi,Xuan Nie,Ye-Zi You,Tamer M. Tamer
Cationic polymers with high charge density could effectively condense the DNA and achieve gene transfection; however, it often brings non-negligible cytotoxicity. Notably, the high charge density gene vector fails in the serum environment, limiting further application in vivo. In this paper, an efficient and reliable non-viral gene vector of poly (amidoamine) (PAA) was designed by introducing diacryolyl-2,6-diaminopyridine (DADAP) onto the PAA backbone through Michael-addition polymerization, which provides high transfection efficiency in a serum-containing environment. Diacryolyl-2,6-diaminopyridine and cationic parts provided multiple interactions between gene vectors and DNA, including hydrogen bond and electrostatic interactions. The introduction of hydrogen bonding can effectively reduce the charge density of polyplexes without reducing the DNA condensing ability, incorporating the diaminopyridine group and cationic part in PAA chains successfully consolidated cellular uptake, endosome destabilization, and transfection efficiency for the PAA/DNA complexes with low cytotoxicity. The constructed vector with multiple interactions presented 6 times higher transfection efficiency in serum-free and 9 times in serum-containing environment than that of branched polyethyleneimine (PEI 25K) in 293T cells in vitro. Therefore, introducing the hydrogen band to form low charge density polyplexes with high transfection efficiency and low cytotoxicity has a great potential in gene delivery.
研究领域:
生物化学
·
分子生物学
·
基因治疗
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 24
·期 1
·275
·
IF 6.208
Fei Li,Honglu Zhao,Yuxin Liu,Jiaqi Zhang,Hongbo Yu
Lytic polysaccharide monooxygenases (LPMOs) have the potential to improve recalcitrant polysaccharide hydrolysis by the oxidizing cleavage of glycosidic bond.Streptomycesspecies are major chitin decomposers in soil ecological environments and encode multiplelpmogenes. In this study, we demonstrated that transcription of thelpmogene,Sclpmo10G, in theStreptomyces coelicolorA3(2) (ScA3(2)) strain is strongly induced by chitin. TheScLPMO10G protein was further expressed inEscherichia coliand characterized in vitro. TheScLPMO10G protein showed oxidation activity towards chitin. Chitinase synergy experiments demonstrated that the addition ofScLPMO10G resulted in a substantial in vitro increase in the reducing sugar levels. Moreover, in vivo the LPMO-overexpressing strainScΔLPMO10G(+) showed stronger chitin-degrading ability than the wild-type, leading to a 2.97-fold increase in reducing sugar level following chitin degradation. The total chitinase activity ofScΔLPMO10G(+) was 1.5-fold higher than that ofScA3(2). In summary,ScLPMO10G may play a role in chitin biodegradation inS. coelicolor, which could have potential applications in biorefineries.Keywords:lytic polysaccharide monooxygenases;transcription;expression;Streptomyces coelicolorA3(2);chitin biodegradation
研究领域:
微生物学
·
生物化学
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DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 4
·2251-2260
·
IF 3.289
Guo Liu,Shengwei Sun,Baoyan Guo,Benchun Miao,Zhen Luo,Zumeng Xia,Danyang Ying,Fei Liu,Bin Guo,Jian Tang,Yong Cao,Jianyin Miao
Casein phosphopeptides (CPPs) have been demonstrated to be calcium chelators. Unfortunately, few studies have been reported on the effects of CPPs on the mechanism of the uptake and absorption of Ca2+ and bone metabolism. In this study, a monomeric peptide fraction isolated by RP-HPLC (F6-1) that possessed high calcium transport capacity in Caco-2 cell monolayers was separated and characterized. The effects of F6-1 on the absorption mechanisms of Ca2+ in a Caco-2 monolayer model and bone metabolism in rats were investigated. F6-1 was isolated by preparative and analytical RP-HPLC. Results for calcium transport suggested that the rates of Ca2+ transportation by F6-1 were approximately 2.57, 2.87 and 2.38 times higher than those in the control group at 30, 60 and 120 min, respectively. Results of ultraviolet (UV) spectroscopy indicated that the intensity of UV absorption changed because of the binding of Ca2+ to F6-1. Analysis of transepithelial electrical resistance (TEER) and the expression of TRPV6 in Caco-2 cells showed that F6-1 was likely to influence the transcellular pathway of intestinal absorption of Ca2+ rather than the paracellular pathway. Furthermore, the F6-1 group (1% Ca, 0.03% F6-1) exhibited increases in serum Ca2+ levels, femur length and femur Ca and decreases in serum alkaline phosphatase (ALP) levels and urinary pyridinoline content in a Sprague-Dawley rat model, which implied that F6-1 was beneficial for bone calcification. Overall, our results suggested that F6-1 enhanced the transport of Ca2+ in Caco-2 cells by affecting the transcellular pathway by upregulating the expression of TRPV6. F6-1 also improved bone formation and prevented bone resorption to benefit bone health in rats, which provided a basis for using F6-1 in calcium supplements or functional foods.
研究领域:
营养学
·
生物化学
·
食品科学
·
生理学
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Suqi Huang,Zili Cui,Lixin Qiao,Gaojie Xu,Jianjun Zhang,Kun Tang,Xiaochen Liu,Qinglei Wang,Xinhong Zhou,Botao Zhang,Guanglei Cui
Due to the ex-situ assembly technology of solid-state electrolyte, solid-state lithium batteries commonly show a poor electrolyte/electrode interfacial contact. Herein, we report an in-situ polymerized poly (tetrahydrofuran) (PTHF)-based solid polymer electrolyte (PTSPE) to resolve the poor interfacial contact problem in solid state lithium batteries . The in-situ formed PTSPE exhibits a high ionic conductivity (2.3 × 10 −4 S cm −1 ) and a wide electrochemical window (4.5 V) at 60 °C. More importantly, the in-situ formed PTSPE improves interfacial compatibility remarkably, suppressing the growth of lithium dendrites . It is revealed that boron trifluoride initiator works as functional additive to form LiF and B-O species at interface layer of lithium metal anode , alleviating lithium dendrites growth and undesired parasitic reactions. An as-assembled LiFePO 4 /Li cell employing this in-situ formed PTSPE delivers a high discharge capacity of 142.3 mA h g −1 even after 100 cycles. The aforementioned results suggest that the in-situ polymerization technology of solid-state electrolyte is very promising for high-performance solid-state lithium batteries.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
TRIBOLOGY INTERNATIONAL
·
2016
·
IF 2.259
Yinglei Wu,Xiangqiong Zeng,Tianhui Ren,Erik de Vries,Emile van der Heide
Graphene oxide (GO) was asymmetric chemically modified with myristyltrimethylammonium bromide (TTAB) to get modified graphene oxide (MGO). This MGO was used as an emulsifier and additive in oil-in-water emulsion. The emulsifying tests showed MGO greatly improved the stability of base emulsion and decreased its droplets size . Tribological test results showed that under the boundary lubrication condition with MGO-emulsion, the friction coefficient (COF) and the wear rate of the steel ball were decreased by about 18% and 48% respectively, comparing with base emulsion lubrication . The tribological mechanism of MGO-emulsion could be explained by the strong film-forming ability on the metal surface; the good lubricity of its small droplets; the formed adsorption film, transfer film and tribofilm between the contact surfaces.
研究领域:
材料科学
·
化学工程
·
机械工程
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-7
·
IF 4.259
Liu Jian,Bi Hong,Cesar Morais Paulo,Zhang Xiang,Zhang Fapei,Hu Lin
Room temperature magnetic ordering is reported for very small carbon dots (CDs), mat-like polyaniline nanofibers (Mat-PANI) and a composite of CDs@Mat-PANI containing 0.315 wt% CDs. We have found saturation magnetization ( M
S
) of CDs, Mat-PANI and CDs@Mat-PANI at 5 (20/300) K equals to 0.0079 (0.0048/0.0019), 0.0116 (0.0065/0.0055) and 0.0349 (0.0085/0.0077) emu/g, respectively. The M
S
enhancement in CDs@Mat-PANI (200% and 40% at 5 K and 300 K, respectively) is attributed to electron transfer from Mat-PANI imine N-atoms to the encapsulated CDs. Changes in M
S
values reveal that 0.81 (0.08) electron/CD is transferred at 5 (300) K, which is supported by observation of CDs photoluminescence (PL) redshift while in CDs@Mat-PANI. Band-bending and bandgap-renormalization calculations are used to predict a redshift of 117 meV at 300 K as a result of the electron transfer, in excellent agreement with the PL data (110 meV). Raman, X-ray diffraction and X-ray photoelectron spectroscopy data are used to confirm the electron transfer process as well as the strong interaction of CDs with PANI within CDs@Mat-PANI, which increases the crystalline domain size of Mat-PANI from about 4.8 nm to 9.2 nm while reducing the tensile strain from about 6.2% to 1.8%.
研究领域:
材料科学
·
物理学
✓ 已验证
DOI
高影响因子
Journal of Inflammation Research
·
2024
·
IF 4.2
Tingting Wang,Xia Wu,Xiaoli Zhao,Jiaqi Li,Jian Yu,Maozheng Sheng,Mingyuan Gao,Yutang Cao,Jiawen Wang,Xiaozhen Guo,Kai Zeng
Purpose Postoperative cognitive dysfunction (POCD) is a central nervous system complication that occurs after anesthesia, particularly among the elderly. However, the neurological pathogenesis of postoperative cognitive dysfunction remains unclear. The aim of this study was to evaluate the effects of sevoflurane exposure on serum metabolites and hippocampal gene expression in elderly patients and aging mice by metabolomics and transcriptomic analysis and to explore the pathogenesis of sevoflurane induced POCD.Patients and Methods Human serum samples from five patients over 60 years old were collected before sevoflurane anesthesia and 1 hour after anesthesia. Besides, mice aged at 12 months (n=6 per group) were anesthetized with sevoflurane for 2 hours or with sham procedure. Subsequently, serum and hippocampal tissues were harvested for analysis. Further investigation into the relationship between isatin and neuroinflammation was conducted using BV2 microglial cells.Results Sevoflurane anesthesia led to the activation of inflammatory pathways, an increased presence of hippocampal astrocytes and microglia, and elevated expression of neuroinflammatory cytokines. Comparative analysis identified 12 differential metabolites that exhibited changes in both human and mouse serum post-sevoflurane anesthesia. Notably, isatin levels were significantly decreased after anesthesia. Notably, isatin levels significantly decreased after anesthesia, a factor known to stimulate proliferation and proinflammatory gene expression in microglia—the pivotal cell type in inflammatory responses.Conclusion Sevoflurane-induced alterations in serum metabolites in both elderly patients and aging mice, subsequently contributing to increased inflammation in the hippocampus.
研究领域:
麻醉学
·
神经科学
·
老年医学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2022
·Vol. 76
·期 4
·2029-2039
·
IF 2.146
Tan Siwei,Ruan Xiaoying,Shao Ji,Shan Xiaoyue,Ye Haipeng,Shi Yanpeng
Self-produced peanut shell AC (activated carbon) and Al(OH) 3 (aluminum hydroxide) were successfully synthesized by ultrasound with CTAB (cetyltrimethyl ammonium bromide), and both new nanocomposite AC@Al(OH) 3 @CTAB were evaluated as adsorbents for absorption of BPA (Bisphenol A) and utilized for the determination of BPA in urine by high-performance liquid chromatography coupled with fluorescence detector. Then the AC and AC@Al(OH) 3 @CTAB nanocomposites were examined by X-ray diffraction spectra, infrared spectrum, scanning electron microscope, transmission electron microscope, X-ray photoelectron spectroscopy techniques and differential pulse voltammetry analysis. The extraction elements such as adsorption pH, time, dose, desorption solvent, elution method were also discussed. Under the optimized extraction condition, the recovery of BPA in urine sample can achieve to 99.5% when using AC@Al(OH) 3 @CTAB as the SPE (solid-phase extraction) absorbents. For the methodological analysis, the linearity was detected in the range of 0.2–50 ng mL −1 ( R 2 =0.999 3), and the limit of detection can be 0.05 ng mL −1 . Within-day and between-day relative standard deviations were less than 3.7 and 5.2%, respectively. The synthesized new nanocomposite (AC@Al(OH) 3 @CTAB) showed excellent absorptive capacity, and the proposed SPE-HPLC-FLD methodology was proved to be efficient, sensitive and operable when using AC@Al(OH) 3 @CTAB as absorbents for BPA detection in complicated biological samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 57
·35917-35927
·
IF 3.108
Jun Wang,Aimin Shi,Dominic Agyei,Qiang Wang
Resveratrol is a high-value bioactive polyphenolic compound with vast applications in functional foods; as such, effective and scalable delivery strategies for this compound are worthy of study. In this research, W/O/W emulsions were successfully prepared using polyglycerol polyricinoleate (PGPR) as a lipophilic emulsifier and Tween 80 as a hydrophilic emulsifier with the goal of developing biocompatible carriers to improve the bioavailability of resveratrol. The effects of the type of emulsifier, the concentration of emulsifier, the ratio of the oil phase to the internal water phase, and homogeneous pressure on the physical properties of the W/O/W emulsions (such as microstructure, droplet size, distribution, zeta potential, viscosity and encapsulation efficiency) were investigated. The optimum processing conditions for preparing W/O/W emulsions are as follows: the ratio of the oil phase to the internal water phase is 80 : 20, the concentrations of lipophilic and hydrophilic emulsifiers are 10 wt% and 5 wt%, and the homogeneous pressures in the first and second steps are 30 MPa and 10 MPa. The optimal preparation process of the W/O/W emulsions was used to encapsulate resveratrol. The results showed that at up to 0.040 wt% in the internal water phase (ethanol), resveratrol could be successfully encapsulated in W/O/W emulsions with an encapsulation efficiency of 99.97 ± 0.001%. Moreover, resveratrol was successfully encapsulated in the internal water phase and oil phase together; thus, it was not necessary to increase the amount of carrier materials. This study provides a novel encapsulation formulation to increase the delivery efficacy of resveratrol.
研究领域:
食品科学
·
药物递送
·
生物技术
✓ 已验证
DOI
高影响因子
Materials
·
2021
·Vol. 14
·期 23
·7332
·
IF 3.623
Yunjie Liu,Huanhuan Yuan,Hui Wang,Zhiwei Wang
We report on thermal stability and phase transition behaviors of triangular Au nanoprisms through in situ heating transmission electron microscopy. With rising temperature, Au nanoprisms exhibit fluctuating surface reconstructions at the corner regions. When a quasi-melting state is reached at the temperature below bulk melting points, the evaporation is initiated commonly at a corner with low curvature and containing sharp intersection points. The subsequent annealing process leads to the gradual evaporation, which, in the absence of thick carbon coverages, is accompanied by marked shape reconstructions. The thermal stability and evaporation behaviors are not evidently regulated by nanoprism aggregations.
研究领域:
材料科学
·
纳米技术
✓ 已验证
DOI
高影响因子
MOLECULAR BIOLOGY AND EVOLUTION
·
2022
·Vol. 39
·期 9
·
IF 8.8
Lu Meng,Li Zhi,Zhu Zi-Yu,Peng Fang,Wang Yang,Li Xi-Yin,Wang Zhong-Wei,Zhang Xiao-Juan,Zhou Li,Gui Jian-Fang
Unisexual animals are commonly found in some polyploid species complexes, and most of these species have had a long evolutionary history. However, their method for avoiding genomic decay remains unclear. The polyploid Carassius complex naturally comprises the sexual amphidiploid C. auratus (crucian carp or goldfish) (AABB) and the gynogenetic amphitriploid C. gibelio (gibel carp) (AAABBB). Recently, we developed a fertile synthetic amphitetraploid (AAAABBBB) male from C. gibelio by incorporating a C. auratus genome. In this study, we generated novel amphitriploids (AAABBB) by backcrossing the amphitetraploid male with the amphidiploid C. auratus. Whole-genome resequencing revealed the genomic changes, including recombination and independent assortment between homologs of C. gibelio and C. auratus. The fertility, sex determination system, oocyte development, and fertilization behaviors of the novel amphitriploids were investigated. Approximately 80% of the novel amphitriploid females recovered the unisexual gynogenesis ability. Intriguingly, two types of primary oocyte (with and without homolog synapsis) were discovered, and their distinct development fates were observed. Type I oocytes entered apoptosis due to improper synaptonemal complex assembly and incomplete double-strand break repair, whereas subsequent type II oocytes bypassed meiosis through an alternative ameiotic pathway to develop into mature eggs. Moreover, gynogenesis was stabilized in their offspring, and a new array of diverse gynogenetic amphitriploid clones was produced. These revealed genomic changes and detailed cytological data provide comprehensive evidence that changes in ploidy drive unisexual and sexual reproduction transition, thereby resulting in genomic diversity and allowing C. gibelio avoid genomic decay.
研究领域:
遗传学
·
进化生物学
·
鱼类学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）