Oxidative Medicine and Cellular Longevity
·
2022
·
IF 7.31
Yuan Liang,Jingqi Zhao,Haoyang Zou,Jie Zhang,Tiehua Zhang
As the main active ingredients of Panax ginseng, ginsenosides possess numerous bioactivities. Epidermal growth factor receptor (EGFR) was widely used as a valid target in anticancer therapy. Herein, the EGFR targeting activities of 20(S)-ginsenoside Rh2 (20(S)-Rh2) and the relationship of their structure-activity were investigated. Homogeneous time-resolved fluorescence assay showed that 20(S)-Rh2 significantly inhibited the activity against EGFR kinase. 20(S)-Rh2 was confirmed to effectively inhibited cell proliferation in a dose-dependent manner by MTT assay. Furthermore, quantitative real-time PCR and western blotting analysis revealed that 20(S)-Rh2 inhibited A549 cells growth via the EGFR-MAPK pathway. Meanwhile, 20(S)-Rh2 could promote cell apoptosis, block cell cycle, and reduce cell migration of A549 cells, respectively. In silico, the result suggested that both hydrophobic interactions and hydrogen-bonding interactions could contribute to stabilize their binding. Molecular dynamics simulation showed that the side chain sugar moiety of 20(S)-Rh2 was too flexible to be fixed at the active site of EGFR. Collectively, these findings suggested that 20(S)-Rh2 might serve as a potential EGFR tyrosine kinase inhibitor.
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高影响因子
RSC Advances
·
2012
·Vol. 2
·期 7
·2666-2670
·
IF 0
Meina Wang,Xiangyou Liu,Chuanbao Cao,Cui Shi
Cu–In–S ternary nanocrystals (NCs), with an average size of less than 10 nm, were synthesized in an aqueous solution containing bovine serum albumin (BSA). X-Ray powder diffraction (XRD) and selected-area electron diffraction (SAED) analyses showed that these NCs featured a roquesite structure. The composition of the NCs could be adjusted by controlling the molar ratio of the starting Cu/In precursors in the reaction solution, which led to a tunable band gap ranging from 1.48 eV to 2.30 eV. Cytotoxicity testing showed that the BSA-stabilized Cu–In–S NCs had little effect on the cell viability, which suggested that they are user-friendly and environmentally benign. With low cost, minimal energy input and environmental impact, this simple approach shows great potential for industrial applications.
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MOLECULES
·
2020
·Vol. 25
·期 3
·674
·
IF 3.267
Jie Liu,Xican Li,Rongxin Cai,Ziwei Ren,Aizhen Zhang,Fangdan Deng,Dongfeng Chen
To elucidate the mechanism of anti-ferroptosis and examine structural optimization in natural phenolics, cellular and chemical assays were performed with 2′-hydroxy chalcone butein and dihydroflavone (S)-butin. C11-BODIPY staining and flow cytometric assays suggest that butein more effectively inhibits ferroptosis in erastin-treated bone marrow-derived mesenchymal stem cells than (S)-butin. Butein also exhibited higher antioxidant percentages than (S)-butin in five antioxidant assays: linoleic acid emulsion assay, Fe3+-reducing antioxidant power assay, Cu2+-reducing antioxidant power assay, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•)-trapping assay, and α,α-diphenyl-β-picrylhydrazyl radical (DPPH•)-trapping assay. Their reaction products with DPPH•were further analyzed using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS). Butein and (S)-butin produced a butein 5,5-dimer (m/z542, 271, 253, 225, 135, and 91) and a (S)-butin 5′,5′-dimer (m/z542, 389, 269, 253, and 151), respectively. Interestingly, butein forms a cross dimer with (S)-butin (m/z542, 523, 433, 419, 415, 406, and 375). Therefore, we conclude that butein and (S)-butin exert anti-ferroptotic action via an antioxidant pathway (especially the hydrogen atom transfer pathway). Following this pathway, butein and (S)-butin yield both self-dimers and cross dimers. Butein displays superior antioxidant or anti-ferroptosis action to (S)-butin. This can be attributed the decrease in π-π conjugation in butein due to saturation of its α,β-double bond and loss of its 2′-hydroxy group upon biocatalytical isomerization.Keywords:butein;(S)-butin;anti-ferroptosis;antioxidant;2′-hydroxy chalcone;isomerization
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Yingqi Chen,Yufei Cheng,Junfeng Zhao,Wenwan Zhang,Jianhua Gao,Hui Miao,Xiaoyun Hu
Antimony sulfide (Sb 2 S 3 ) is a relatively abundant and environmentally friendly emerging photovoltaic material, which has been gradually applied in solar cells and photocatalysis. It has high light absorption capacity, but it suffers many deep-level defects and is prone to recombination of electron-hole pairs within itself. Here, by constructing the Sb 2 S 3 /CdIn 2 S 4 S-scheme heterojunction, we avoided the problem that electrons and holes cannot be separated and transported effectively due to many Sb 2 S 3 defects (more recombination centers), and improved its application in the field of photoelectrochemical water splitting. Meanwhile, in order to further improve the performance of Sb 2 S 3 /CdIn 2 S 4 photoelectrode, we introduced CdS energy platform between Sb 2 S 3 and CdIn 2 S 4 to form a Sb 2 S 3 /CdS/CdIn 2 S 4 cascaded S-scheme heterojunction. Compared with Sb 2 S 3 monomer, Sb 2 S 3 /CdS/CdIn 2 S 4 had higher absorbance intensity, IPCE value, ABPE value, and lower charge transfer resistance. In addition, the photocurrent density of the Sb 2 S 3 /CdS/CdIn 2 S 4 photoelectrode was about 4.20 mA/cm 2 (1.23 V vs. RHE), which was 1.3 times higher than that of the Sb 2 S 3 /CdIn 2 S 4 photoelectrode (3.29 mA/cm 2 ) and 3.2 times higher than that of monomer Sb 2 S 3 photoelectrode (1.32 mA/cm 2 ). This method offers new prospects for optimizing the performance of antimony chalcogenides photoelectrodes for photoelectrochemical water splitting.
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高影响因子
JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 28
·期 8
·1-9
·
IF 3.097
He Tingwei,Liu Tao,Huang Junyao,Wang Xueli,He Yong,Ma Bomou
In this work, a novel poly(ester amide)s (PEAs) was designed and synthesized from dimethyl terephthalate (DMT), 3-amino-1-propanol (AP) and adipic acid (AA). Firstly, the aromatic diol monomer (BHTT) was synthesized by DMT and AP, it was characterized by 1 H NMR, 13 C NMR, FT-IR and DSC, respectively. Then, the BHTT and AA were used to synthesize the semi-aromatic PEAs with alternating structure by melt polymerization. The effects of polymerization temperature and time on the crystallization, thermal stability and amide transesterification of PEAs were fully investigated. It was found that the prepared PEAs are semi-crystalline polymer with melting point around 154–165 °C and decomposition temperature around 377–392 °C. Most important, the chemical structure of PEAs synthesized under different conditions was explored by 1 H NMR, 13 C NMR and DEPT spectra, which confirms the occurrence of amide transesterification during the melt polymerization process.
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高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Wenwei Lin,Wen-Ti Guo,Liquan Yao,Jianmin Li,Limei Lin,Jian-Min Zhang,Shuiyuan Chen,Guilin Chen
Hydrothermal deposition is emerging as a highly potential route for antimony-based solar cells, in which the Sb2(S,Se)3 is typically in situ grown on a common toxic CdS buffer layer. The narrow band gap of CdS causes a considerable absorption in the short-wavelength region and then lowers the current density of the device. Herein, TiO2 is first evaluated as an alternative Cd-free buffer layer for hydrothermally derived Sb2S3 solar cells. But it suffers from a severely inhomogeneous Sb2S3 coverage, which is effectively eliminated by inserting a Zn(O,S) layer. The surface atom of sulfur in Zn(O,S) uniquely provides a chemical bridge to enable the quasi-epitaxial deposition of Sb2S3 thin film, confirming by both morphology and binding energy analysis using DFT. Then the results of the first-principles calculations also show that Zn(O,S)/Sb2S3 has a more stable structure than TiO2/Sb2S3. The resultant perfect Zn(O,S)/Sb2S3 junction, with a suitable band alignment and excellent interface contact, delivers a remarkably enhanced JSC and VOC for Sb2S3 solar cells. The device efficiency with the TiO2/Zn(O,S) buffer layer boosts from 0.54% to 3.70% compared with the counterpart of TiO2, which has a champion efficiency of Cd-free Sb2S3 solar cells with a structure of ITO/TiO2/Zn(O,S)/Sb2S3/Carbon/Ag by in situ hydrothermal deposition. This work provides a guideline for the hydrothermal deposition of antimony-based films upon a nontoxic buffer layer.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 16
·13303-13315
·
IF 2.779
Yang Lu,Wang Juan,Zhang Yi,Zhou Binhua,Tan Pengfei,Pan Jun
Developing semiconductor-based photocatalytic systems with wide spectral response and effective charge separation is critical to rapidly and efficiently remove antibiotics residues in water. Herein, S-scheme BiOCl/CdS nanocomposites have been successfully designed and synthesized by decorating CdS nanoparticles on the two-dimensional BiOCl nanosheets through a simple hydrothermal method. The composition, microstructure and optical properties of the prepared samples were thoroughly investigated. Tetracycline hydrochloride (TC-HCl) was used as the model pollutant to estimate the photocatalytic properties of BiOCl/CdS catalysts. All the obtained BiOCl/CdS nanocomposites showed enhanced photocatalytic removal of TC-HCl. Especially, the optimal sample showed the highest photodegradation rate, which was 2.94 and 4.73 times that of bare CdS and BiOCl, respectively. The improved degradation of TC-HCl for BiOCl/CdS photocatalysts was ascribed to the increased light absorption as well as the boosted separation and transfer of carriers. Based on the results of active group capture experiment, transient photocurrent, electrochemical impedance spectroscopy and band structure, the S-scheme charge transfer mechanism for TC-HCl degradation in this BiOCl/CdS photocatalytic system was also discussed.
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高影响因子
MOLECULAR CELL
·
2023
·
IF 16
Xue Huang,Jia Yao,Lu Liu,Jing Chen,Ligang Mei,Jingjing Huangfu,Dong Luo,Xinyi Wang,Changhai Lin,Xiaorong Chen,Yi Yang,Sheng Ouyang,Fujing Wei,Zhuolin Wang,Shaolin Zhang,Tingxiu Xiang,Dante Neculai,Qiming Sun,Eryan Kong,Edward W. Tate,Aimin Yang
p62 is a well-characterized autophagy receptor that recognizes and sequesters specific cargoes into autophagosomes for degradation. p62 promotes the assembly and removal of ubiquitinated proteins by forming p62-liquid droplets. However, it remains unclear how autophagosomes efficiently sequester p62 droplets. Herein, we report that p62 undergoes reversible S -acylation in multiple human-, rat-, and mouse-derived cell lines, catalyzed by zinc-finger Asp-His-His-Cys S -acyltransferase 19 (ZDHHC19) and deacylated by acyl protein thioesterase 1 (APT1). S -acylation of p62 enhances the affinity of p62 for microtubule-associated protein 1 light chain 3 (LC3)-positive membranes and promotes autophagic membrane localization of p62 droplets, thereby leading to the production of small LC3-positive p62 droplets and efficient autophagic degradation of p62-cargo complexes. Specifically, increasing p62 acylation by upregulating ZDHHC19 or by genetic knockout of APT1 accelerates p62 degradation and p62-mediated autophagic clearance of ubiquitinated proteins. Thus, the protein S -acylation-deacylation cycle regulates p62 droplet recruitment to the autophagic membrane and selective autophagic flux, thereby contributing to the control of selective autophagic clearance of ubiquitinated proteins.
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高影响因子
ChemPlusChem
·
2013
·Vol. 79
·期 2
·211-216
·
IF 0
Wentao Hao,Songyan Ding,Liang Zhang,Wei Liu,Wen Yang
Graphical Mother-of-pearl films : Flexible, transparent, and glossy nanocomposite films have been fabricated through vacuum-aided filtration self-assembly of hyperbranched poly(amido amine)s (HPAMAMs) and clay nanosheets. Such hybrid films show strong fluorescence properties and high strength (see figure). As a result of the good biocompatibility of HPAMAMs, the hybrid films are promising in biomedical research. Inspired by the hierarchical structure of nacre, lots of functional nanocomposite films have been fabricated through finely tuning the composition and structure. To broaden their application in biological and biomedical research, it is highly demanded to develop a new strategy to fabricate nanocomposite films that are both biocompatible and functional. Herein, hyperbranched poly(amido amine)s (HPAMAMs) have been assembled with clay nanosheets. The obtained nanocomposite films show good mechanical performance and fluorescence properties. Analysis of the structure of the nanocomposite films indicates that the HPAMAMs and the clay nanosheets are stacked together in an orderly manner. The clay nanosheets align in the direction parallel to the film surface. Because of the inherent biocompatibility and autofluorescence property of the HPAMAMs, such composite films are thought to be very promising in applications requiring biocompatibility, fluorescence, and good mechanical performance. The method should provide a new route to the fabrication of functional nacrelike composite materials.
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SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2021
·
IF 7.267
Xiaowei Zhou,Chao Gao,Hao Luo,Zhiwei Liu,Haixu Liu,Haifeng Gao,Qing Zhou,Wenbo Li,Xiaoyun Teng,Wei Yu
In this paper, we reported a modified preparation process for Cu 2 ZnSn(S,Se) 4 (CZTSSe) films. For this process, Al 2 O 3 capping layers were deposited on precursor films before selenization. It was found the capping layer can prevent the formation of separate Cu x Se phase on top of the film and facilitate a homogeneous growth of the crystalline grains during selenization. Detailed characterizations revealed the reason: the Al 2 O 3 capping layers can inhibit the incorporation of Se into the film in low-temperature region (below 350 °C). So the reaction between Se and Cu, which is thermodynamically preferential in this temperature region, can be prevented. As the disappearance of Cu x Se, the traditional top-to-down crystallization mode was substituted by a homogeneous crystallization mode. Therefore, CZTSSe films with homogeneous crystallization properties were achieved. By using the capping layers, the efficiencies of the solar cells were improved. In particular, the open-circuit voltage (V OC ) of the solar cell increased by ~18% by using the capping layers.
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高影响因子
NUCLEIC ACIDS RESEARCH
·
2019
·Vol. 47
·期 22
·11514-11526
·
IF 11.147
Jie Jialong,Xia Ye,Huang Chun-Hua,Zhao Hongmei,Yang Chunfan,Liu Kunhui,Song Di,Zhu Ben-Zhan,Su Hongmei
Phosphorothioate (PS) modifications naturally appear in bacteria and archaea genome and are widely used as antisense strategy in gene therapy. But the chemical effects of PS introduction as a redox active site into DNA (S-DNA) is still poorly understood. Herein, we perform time-resolved spectroscopy to examine the underlying mechanisms and dynamics of the PS oxidation by potent radicals in free model, in dinucleotide, and in S-oligomer. The crucial sulphur-centered hemi-bonded intermediates -P–S∴S–P- were observed and found to play critical roles leading to the stable adducts of -P–S–S–P-, which are backbone DNA lesion products. Moreover, the oxidation of the PS moiety in dinucleotides d[GPSG], d[APSA], d[GPSA], d[APSG] and in S-oligomers was monitored in real-time, showing that PS oxidation can compete with adenine but not with guanine. Significantly, hole transfer process from A+• to PS and concomitant -P–S∴S–P- formation was observed, demonstrating the base-to-backbone hole transfer unique to S-DNA, which is different from the normally adopted backbone-to-base hole transfer in native DNA. These findings reveal the distinct backbone lesion pathway brought by the PS modification and also imply an alternative -P–S∴S–P-/-P–S–S–P- pathway accounting for the interesting protective role of PS as an oxidation sacrifice in bacterial genome.
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高影响因子
CARBON
·
2015
·
IF 6.196
Feng Wu,Lili Shi,Daobin Mu,Hongliang Xu,Borong Wu
A novel hierarchical structure carbon/sulfur composite is presented based on carbon fiber matrices, which are synthesized by electrospinning. The fibers are constituted with hollow graphitized carbon spheres formed using catalytic Ni nano-particles as hard templates. Sulfur is loaded to the carbon substrates via thermal vaporization. The structure and composition of the hierarchical carbon fiber/S composite are characterized with X-ray diffraction, scanning electron microscopy, transmission electron microscopy , Raman spectroscopy , and nitrogen adsorption isotherms . The electrochemical performance is evaluated by cyclic voltammetry and galvanostatic charge–discharge. The results exhibit an initial discharge capacity of 845 mA h g −1 at 0.25 C (420 mA g −1 ), with a retention of 77% after 100 cycles. A discharge capacity of 533 mA h g −1 is still attainable when the rate is up to 1.0 C. The good cycling performance and rate capability are contributed to the uniform dispersion of sulfur, the conductive network of carbon fibers and hollow graphitized carbon spheres.
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高影响因子
危险品化学品经营许可证（带存储）