Evidence-based Complementary and Alternative Medicine
·
2022
·
IF 2.65
Yu Zhou,Zijian Zhang,Yingying Sun,Dandan Zhou,Yang Zhao,Jianxiang Li,Weifeng Guo
Background. Liangxue Tongyu prescription (LTP) is a commonly used formula for acute intracerebral hemorrhage (AICH) in clinical practice that has significant ameliorative effects on neurological deficits and gastrointestinal dysfunction, yet the mechanism remains elusive. The aim of this study was to investigate the pathway by which LTP alleviates brain damage in AICH rats. Methods. The AICH rat models were established by autologous caudal arterial blood injection. The neurological function scores were evaluated before and after treatment. The water content and the volume of Evans blue staining in the brain were measured to reflect the degree of brain damage. RT-PCR was used to detect the inflammatory factors of the brain. Western blotting was used to detect the expression of the tight junction proteins zonula occludens 1 (ZO-1), occludin (OCLN), and claudin (CLDN) in the brain and colon, followed by mucin 2 (MUC2), secretory immunoglobulin A (SIgA), and G protein-coupled receptor 43 (GPR43) in the colon. Flow cytometry was used to detect the ratios of helper T cells 17 (Th17) and regulatory T cells (Treg) in peripheral blood, and the vagus nerve (VN) discharge signals were collected. Results. LTP reduced the brain damage of the AICH rats. Compared with the model group, LTP significantly improved the permeability of the colonic mucosa, promoted the secretion of MUC2, SigA, and GPR43 in the colon, and regulated the immune balance of peripheral T cells. The AICH rats had significantly faster VN discharge rates and lower amplitudes than normal rats, and these abnormalities were corrected in the LTP and probiotics groups. Conclusion. LTP can effectively reduce the degree of brain damage in AICH rats, and the mechanism may be that it can play a neuroprotective role by regulating the function of the intestinal mucosal barrier.
研究领域:
神经科学
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药理学
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免疫学
·
胃肠病学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.2
Feng He,Aihong Wu,Chuanlai Xu,Hua Kuang,Aihua Qu
Fenazaquin (FEN), a widely used quinazoline acaricide, poses potential risks to public health and ecosystems due to its residue accumulation in food and the environment, thus demanding rapid and reliable detection methods. Herein, novel FEN haptens were rationally designed based on computational chemistry to successfully generate a highly specific and sensitive monoclonal antibody. The antibody exhibited a half-inhibitory concentration of 1.90 ng/mL. A gold nanoparticle-based dual-T-line lateral flow immunochromatographic assay was established for the rapid detection of FEN in lake water, soil, apples, and tea within 20 min. The detection limits were 1.74, 2.29, 4.38, and 2.64 μg/kg, with quantitative ranges of 6.12–450.44, 7.38–404.59, 11.49–309.78, and 8.16–385.02 μg/kg, respectively. Spiked recovery and blind sample tests showed excellent agreement with instrumental analysis. This study provides a rational hapten design strategy and a practical tool for field screening of FEN in food and environmental matrices.
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高影响因子
INFLAMMATION
·
2020
·Vol. 43
·期 5
·1780-1788
·
IF 3.212
Zhang Liming,Huang Yang,Cui Xiaoxiao,Tan Xiao,Zhu Ying,Zhou Wenjun,Wang Chaokui,Yuan Gangxiang,Cao Qingfeng,Su Guannan,Kijlstra Aize,Yang Peizeng
Previous studies have pointed out that indoleamine 2,3-dioxygenase (IDO), the rate-limiting enzyme initiating tryptophan metabolism, plays a role in the regulation of the immune system. This project was designed to investigate the potential role of IDO in monocyte-derived dendritic cells (moDCs) obtained from active Vogt-Koyanagi-Harada (VKH) disease patients. In this study, we found that the IDO mRNA expression and enzyme activity were increased in active VKH patients as compared with healthy controls and patients in remission. To investigate the role of IDO in immune regulation, an effective inhibitor 1-methyl-L-tryptophan (1-MT) was used to suppress its activity in DCs. The results showed that inhibition of IDO with 1-MT significantly decreased the expression of DC marker CD86. IDO inhibition did not affect the cytokine production of IL-6, IL-1β, TNF-α, IL-10, and TGF-β in DCs. Downregulation of IDO in DCs also led to the reduction of regulatory T (Treg) cells and an increased CD4 + T cell proliferation. Treatment with 1-MT did not affect the phosphorylation of the MAPK pathway in DCs. In general, our study suggests that IDO may play an important role in the pathogenesis of VKH disease by regulating DC and CD4 + T cell function. Tryptophan deficiency and kynurenine accumulation may account for the complicated effects of IDO. Further research is needed to study the precise tryptophan metabolites that may limit immune responses in VKH disease.
研究领域:
免疫学
·
眼科
·
分子生物学
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PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2024
·
IF 2.2
Yufeng Tang,Qichao Yang,Zhanping Qiao
The phase equilibrium of ternary systems RbX/CsX + CuX 2 + H 2 O (X = Cl, Br) at T = 298.15 K were investigated by the isothermal dissolution equilibrium method, in which the composition of equilibrium solid phases was determined by the Schreinemaker’s wet residue method. In the system RbCl + CuCl 2 + H 2 O, there were three crystallization regions corresponding to RbCl, Rb 2 CuCl 4 ·2H 2 O and CuCl 2 ·2H 2 O, respectively. Five crystallization fields corresponding to CsCl, CsCuCl 3 , Cs 3 Cu 2 Cl 7 ·2H 2 O, Cs 2 CuCl 4 and CuCl 2 ·2H 2 O were formed in the system CsCl + CuCl 2 + H 2 O. The ternary system RbBr + CuBr 2 + H 2 O had three crystalline regions corresponding to RbBr, Rb 2 CuBr 4 ·2H 2 O and CuBr 2 . In the system CsBr + CuBr 2 + H 2 O, the solid-phases of CsCuBr 3 , Cs 2 CuBr 4 , besides CsBr and CuBr 2 , were discovered. The results were compared with available literature data for cesium bromide/copper bromide salt-water system. The seven new solid phase were characterized using the X-ray diffraction method and thermogravimetric/differential analysis. The dissolution enthalpies of Rb 2 CuCl 4 ·2H 2 O, Rb 2 CuBr 4 ·2H 2 O, CsCuCl 3 , Cs 3 Cu 2 Cl 7 ·2H 2 O, Cs 2 CuCl 4 , CsCuBr 3 and Cs 2 CuBr 4 at T = 298.15 K were measured. Their standard enthalpies of formation were obtained, and the results were in order with −(1714.4 ± 3.2) kJ·mol −1 , −(1533.6 ± 3.3) kJ·mol −1 , −(697.8 ± 3.1) kJ·mol −1 , −(2418.9 ± 6.1) kJ·mol −1 , −(1134.8 ± 3.2) kJ·mol −1 , −(563.5 ± 3.2) kJ·mol −1 and −(966.2 ± 3.3) kJ·mol −1 , respectively.
研究领域:
物理化学
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无机化学
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热力学
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高影响因子
Journal of Manufacturing Processes
·
2026
·
IF 7.8
Yifei Yan,Chuanzhen Huang,Zhenyu Shi,Zhen Wang,Longhua Xu,Shuiquan Huang,Meina Qu,Zhengkai Xu,Dijia Zhang,Baosu Guo,Tianye Jin,Xiaodan Wang,Hanlian Liu,Dun Liu,Peng Yao
High-solid-loading ceramic vat photopolymerization (VPP) enables dense ceramic components, but dimensional control remains difficult because particle-induced scattering redistributes exposure energy and alters curing geometry. Existing working-curve descriptions, simplified scattering models, and empirical compensation schemes provide only limited predictive connection between slurry optical characteristics and printed feature dimensions when dependent scattering becomes significant. Here, a coupled multiple-sphere T-matrix and Monte Carlo ray-tracing (MSTM-MCRT) approach was developed for curing-geometry prediction and contour compensation. Full-wave MSTM calculations were used to obtain dependent-scattering radiative properties, including scattering, absorption, and angular redistribution, for dense particle assemblies. These properties were then incorporated into a three-dimensional MCRT model to predict spatial dose distribution, cure depth, cure width, and cross-sectional curing contour. The predicted curing dimensions were further converted into local boundary offsets for model-guided compensation of contour-defined microfeatures and internal channels. For the investigated Al 2 O 3 –resin slurry over 10–50 vol% solid loading, comparison with independent-scattering approximation and structure-factor approximation baselines shows that the advantage of MSTM becomes especially evident at Φ ≥ 40 vol%. Experiments showed that the predicted cure depth and cure width remained within the ±5% reference band in the relative error analysis. The approach also compensated square openings, circular openings, and a serpentine microchannel, yielding a 791 μm opening for an 800 μm target. This study establishes a physics-based route from dense-particle optical response to curing-geometry prediction and contour compensation, providing guidance for exposure design, slurry-formulation assessment, and dimensional control in high-solid-loading ceramic VPP.
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高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Qinglin Wang,Zhuojun Wei,Jin Li,Danyang Feng,An Feng,Hui Zhang
Water-soluble captopril-capped atomically precise Pd nanoclusters (Pd17Capt8 NCs: 1.3 ± 0.5 nm) produced by a simple chemical reduction were supported on preprepared hybrid Co3Al-layered double hydroxide/reduced graphene oxide (Co3Al-LDH/rGO) by a pH-adjusted electrostatic adsorption strategy followed by proper calcinations, giving a series of novel catalysts x-PdNCs/CoAl(O)/rGO-T (x (Pd loading) = 0.09, 0.17, 0.43 wt % (ICP), T = 230, 250, 280, 300, 320 °C). The characterization results show that the as-obtained catalysts possess the hierarchical nanosheet array morphology. Pd NCs with a size of ∼1.3 to 1.8 nm are highly distributed at the edge sites of the CoAl(O) nanosheets. All of the x-PdNCs/CoAl(O)/rGO-T catalysts show superior catalytic efficiency for the conversion of 4-nitrophenol to 4-aminophenol, particularly 0.17-PdNCs/CoAl(O)/rGO-300 possesses the highest performance with a turnover frequency (TOF) of 30 042 h–1, which is the highest among the reported Pd-based catalysts so far. The superior activity of 0.17-PdNCs/CoAl(O)/rGO-300 can be owing to ultrafine Pd NCs with a clean surface, the strongest PdNCs–Co2+-OH(LDH)–rGO three-phase synergy, and the much improved adsorption of the substrate via π–π stacking upon nanosheet array morphology. Meanwhile, 0.17-PdNCs/CoAl(O)/rGO-300 exhibits excellent catalytic activities for various nitroarenes and anionic azo dyes as well as good reusability with the complete reduction of 4-nitrophenol (4-NP) within 90 s after 10 successive runs. The present work provides not only a simple and convenient strategy for the synthesis of clean, efficient, and environmentally friendly supported metal nanocluster catalysts but also a new idea for the efficient catalytic degradation of environmental pollutants.
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高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2022
·
IF 3.269
Lingyan Gui,Dongdong Li,Jiangtao Wu,Xianyang Meng
The apparent molar heat capacities of LiBO 2 (aq), NaBO 2 (aq), KBO 2 (aq), Na 2 B 4 O 7 (aq) and K 2 B 4 O 7 (aq) solutions at molalities from 0.01 to 2.6 mol·kg −1 , and at temperatures T = (298.12, 313.13 and 328.14) K have been determined using a Picker flow calorimeter built in this work. Further, the standard heat capacities of these solutes were derived from the experimental data by extrapolation to infinite dilution using the Pitzer ion-interaction model. The group contribution method is used to calculate the standard molar heat capacities of tetraborate ion in three equilibria for different assumptions, among which the prediction on the assumption that the standard molar heat capacity of reaction for the association reaction is zero is the most reasonable, and the results are in good agreement with the experimental data.
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高影响因子
Bioengineered
·
2021
·
IF 3.269
Haixia Liu,Mei Song,Xiaoyan Sun,Xin Zhang,Huayan Miao,Yankui Wang
Cervical cancer (CC) is the fourth most common cancers among women worldwide. T-box transcription factor 1 (TBX1), a member of the T-box family, has anti-tumor effects in some types of cancer, but its role in CC is yet unknown. The aim of this study is to investigate the functions and underlying mechanisms of TBX1 in CC. Online database UALCAN showed that TBX1 was down-regulated in CC tissues compared with normal tissues and patients with lower TBX1 expression level had a poor prognosis. TBX1 overexpression significantly decreased the proliferation, migration, and invasion of Hela and SiHa cells. Conversely, cell apoptosis and chemosensitivity to cisplatin were promoted in TBX1-overexpressing CC cells. Moreover, up-regulation of TBX1 inhibited both AKT and MAPK signaling pathways. Furthermore, dual luciferase report assay indicated that TBX1 could directly bind to miR-6727-5p. In addition, TBX1 expression was inhibited by miR-6727-5p mimic and up-regulated by miR-6727-5p inhibitor. Knockdown of TBX1 reversed the inhibitory effect of the miR-6727-5p inhibitor on CC cells. This study demonstrates that TBX1, a target gene of miR-6727-5p, acts as a tumor suppressor in CC, indicating that TBX1 may be a new target for CC therapy.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2020
·
IF 2.369
Xiaomei Qin,Yapei Chen,Shihao Yang,Xiaoyun Qin,Jianbo Zhao,Wenjun Fang
Measurements on densities, viscosities, and refractive indices at atmospheric pressure for binary mixtures of 1,3-dimethyladamantane (1,3-DMA), 1,3,5-trimethyladamantane (1,3,5-TMA), and 1-ethyladamantane (1-EA) with n-nonane or n-undecane are carried out over the whole composition range at temperatures T = 293.15–343.15 K and atmospheric pressure. The excess molar volume (VmE), deviation in viscosity (Δη), deviation in refractive index (ΔnD), and deviation in molar refraction (ΔR) for the six mixtures are calculated and then fitted to the Redlich–Kister polynomial. The VmE values are all negative over the entire range of mole fractions and decrease with increasing temperature for the six binary systems. The absolute values of Δη for the mixtures decrease obviously with increasing temperature. The ΔnD values with the volume fraction for the six systems are all positive over the entire composition range. Density, viscosities, and refractive index of the six systems are correlated by the Jouyban–Acree model. The experimental results could be useful for the development of high-energy-density hydrocarbon fuels.
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高影响因子
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
·
2016
·Vol. 120
·期 6
·560-570
·
IF 3.097
Hongjun Zhang,Baoling Ju,Xiaoli Zhang,Yanfei Zhu,Ying Nie,Yuanhong Xu,Qiuxia Lei
Magnolol is a pharmacological biphenolic compound extracted from Chinese herb Magnolia officinalis , which displays anti-inflammatory and antioxidant effects. This study was aimed at exploring the potential effect of magnolol on immune-related liver fibrosis. Herein, BALB/c mice were injected with concanavalin A (ConA, 8 mg/kg/week) up to 6 weeks to establish hepatic fibrosis, and magnolol (10, 20, 30 mg/kg/day) was given to these mice orally throughout the whole experiment. We found that magnolol preserved liver function and attenuated liver fibrotic injury in vivo . In response to ConA stimulation, the CD4 + T cells preferred to polarizing towards CD4 + T helper 17 (Th17) cells in liver. Magnolol was observed to inhibit Th17 cell differentiation in ConA-treated liver in addition to suppressing interleukin (IL)-17A generation. Hepatic stellate cells were activated in fibrotic liver as demonstrated by increased alpha smooth muscle actin (α-SMA) and desmin. More transforming growth factor (TGF)-β1 and activin A were secreted into the serum. Magnolol suppressed this abnormal HSC activation. Furthermore, the phosphorylation of Smad3 in its linker area (Thr179, Ser 204/208/213) was inhibited by magnolol. In vitro , the recombinant IL-17A plus TGF-β1 or activin A induced activation of human LX2 HSCs and promoted their collagen production. Smad3/Smad4 signalling pathway was activated in LX2 cells exposed to the fibrotic stimuli, as illustrated by the up-regulated phospho-Smad3 and the enhanced interaction between Smad3 and Smad4. These alterations were suppressed by magnolol. Collectively, our study reveals a novel antifibrotic effect of magnolol on Th17 cell-mediated fibrosis.
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MOLECULAR IMMUNOLOGY
·
2024
·
IF 3.2
Zhou Si Yun,Song Zhihua,Tian Xuelian,Xia Min,Hu Rongjing,Luo Mei
Chlamydia trachomatis (CT) is the leading cause of bacterial sexually transmitted diseases worldwide, which can cause diseases such as pelvic inflammatory disease, and cervical and fallopian tube inflammation, and poses a threat to human health. Rosmarinic acid (RosA) is an active ingredient of natural products with anti-inflammatory and immunomodulatory effects. This study aimed to investigate the role of RosA in inhibiting autophagy-regulated immune cells-CD8+ T cells via the Ras/Raf/MEK/ERK signaling pathway in a CT-infected mouse model. Mice were inoculated with CT infection solution vaginally, and the mechanistic basis of RosA treatment was established using H&E staining, flow cytometry, immunofluorescence, transmission electron microscopy, and western blot. The key factors involved in RosA treatment were further validated using the MEK inhibitor cobimetinib. Experimental results showed that both RosA and the reference drug azithromycin could attenuate the pathological damage to the endometrium caused by CT infection; flow cytometry showed that peripheral blood CD8+ T cells increased after CT infection and decreased after treatment with RosA and the positive drug azithromycin (positive control); immunofluorescence showed that endometrial CD8 and LC3 increased after CT infection and decreased after RosA and positive drug treatment; the results of transmission electron microscopy showed that RosA and the positive drug azithromycin inhibited the accumulation of autophagosomes; western bolt experiments confirmed the activation of autophagy proteins LC3Ⅱ/Ⅰ, ATG5, Beclin-1, and p62 after CT infection, as well as the inhibition of Ras/Raf/MEK/ERK signaling. RosA and azithromycin inhibition of autophagy proteins activates Ras/Raf/MEK/ERK signaling. In addition, the MEK inhibitor cobimetinib attenuated RosA's protective effect on endometrium by further activating CD8+ T cells on a CT-induced basis, while transmission electron microscopy, immunofluorescence, and western blots showed that cobimetinib blocked ERK signals activation and further induced phagocytosis on a CT-induced basis. These data indicated that RosA can activate the Ras/Raf/MEK/ERK signaling pathway to inhibit autophagy, and RosA could also regulate the activation of immune cells-CD8+T cells to protect the reproductive tract of CT-infected mice.
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高影响因子
Journal of Cancer
·
2019
·
IF 3.182
Wanru Ma,Juan Ma,Ting Lei,Man Zhao,Man Zhang
To investigate whether CD155 is an attractive target for T cell-mediated immunotherapy against human bladder cancer, we examined the novel bispecific antibody anti-CD3 x anti-CD155 (CD155Bi-Ab) for its ability to redirect activated T cells (ATCs) to target bladder cancer cells was examined. Expression of CD155 was detected by flow cytometry on the surface of bladder cancer cells, including T24 and Pumc-91 cells, and their chemotherapeutic drug-resistant counterparts. ATCs generated from healthy donors were stimulated with anti-CD3 monoclonal antibody, anti-CD28 monoclonal antibody and interleukin-2 (IL-2) for 14 days. The cytotoxic activity of ATCs armed with CD155Bi-Ab against bladder cancer cells was detected by LDH and luciferase quantitative assay. Furthermore, ATCs generated from bladder cancer patients were also armed with CD155Bi-Ab to verity the cell killing by the same methods. In contrast to unarmed ATCs, CD155Bi-armed ATCs against bladder cancer cells were increased cytotoxic activity at effector/target (E/T) ratios of 5:1, 10:1, and 20:1, with more IFN-γ, TNF-α secreting. It is worth noting that in spite of the presence of immunosuppression in bladder cancer patients and the drug resistance in chemotherapeutic drug-resistant cancer cell lines, not only the anti-tumor effect of CD155Bi-armed ATCs generated from bladder cancer patients still showed significantly but only higher level of activation marker CD69 was expressed. Taken together, our results suggest that CD155 is an effective target for the CD155-positive bladder cancer. And CD155Bi-Ab-armed ATCs are promisingly to provide a novel strategy for current CD155-positive bladder cancer therapy.
研究领域:
肿瘤学
·
免疫疗法
·
分子生物学
·
癌症研究
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PubMed
高影响因子
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