CATALYSIS LETTERS
·
2021
·Vol. 151
·期 9
·2527-2536
·
IF 3.186
Du Hui-Jun,Luo Wei,Appiah Bright,Zou Zhi-Cheng,Yang Zhong-Hua,Zeng Rong,Luo Li
Abstract The intracellular NADPH insufficient supply is the main bottleneck to the synthesis of chiral alcohols by asymmetric reduction with whole-cell catalysis. Herein, we provide a novel strategy to strengthen intracellular NADPH supply through introducing an NADP + -dependent glyceraldehyde 3-phosphate dehydrogenase ( gap B from Bacillus subtilis 168) into the Embden-Meyerhof pathway and a NAD kinase ( yfj B from E. coli MG1655) to further enhance the NADP(H) pool. A recombinant E. coli ( E. coli BL21 (DE3)/pETDuet-1- gap B- yue D&pET28a- yfj B) was constructed to co-express gap B and yfj B with a carbonyl reductase gene yue D together. The result showed that the intracellular NADPH amount increased by 134.4% with the strategy. To the model reaction (asymmetric reduction of acetophenone to S -phenyl ethanol), the yield was 3.7-fold with this strategy compared to the control. This provides a technological route for strengthening the intracellular NADPH supply in E. coli for biocatalysis and biosynthesis. Graphic Abstract
✓ 已验证
DOI
高影响因子
AAPS PHARMSCITECH
·
2020
·Vol. 21
·期 5
·1-13
·
IF 2.401
Liu Dandan,Qiao Sen,Cheng Bingchao,Li Dongyang,Chen Jisuan,Wu Qingyin,Pan Hao,Pan Weisan
Despite that either non-covalent or covalent attachment of hydrophilic polymers or surfactants onto nanodiamonds (NDs) could overcome the shortcomings of being a drug delivery system, it is hard to draw a definite conclusion which strategy is more effective. Hence, with the purpose of comparing the influence of different coating approach of NDs on the oral delivery efficiency of water-insoluble model drug curcumin (CUR), NDs were firstly modified with d -α-tocopheryl polyethylene glycol 1000 succinate (TPGS) via non-covalent or covalent conjugation method, and then loaded with CUR (CUR@NDs-COOH/TPGS or CUR@NDs-TPGS). In comparison with the core-shell-structured CUR@NDs-COOH/TPGS, CUR@NDs-TPGS were irregular in shape with dense TPGS film, and exhibited smaller size, more negatively potential, and higher drug loading efficiency. The covalent connection group also showed higher anti-cancer activity, cellular uptake, and permeability through the Caco-2 cell monolayers, as well as favorable distribution, penetration, and retention in rat intestines. The oral bioavailability study in rats demonstrated that CUR@NDs-TPGS showed significantly greater C max and AUC 0− t in contrast with CUR suspension and the TPGS-coated ones, respectively. The findings illustrated that covalent grafting TPGS onto the surface of NDs possesses better efficacy and biocompatibility on oral delivery of poorly soluble drug CUR than pristine and non-covalent coated nanoparticles.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2021
·Vol. 23
·期 6
·1-16
·
IF 2.253
Wang Qilong,Liu Jing,Liu Jian,Thant Yaminn,Weng Wen,Wei Chunmei,Bao Rui,Adu-Frimpong Michael,Yu Qingtong,Deng Wenwen,Cao Xia,Toreniyazov Elmurat,Ji Hao,Xu Ximing,Yu Jiangnan
Recently, bisdemethoxycurcumin (BDMC), a constituent of turmeric, has been studied widely in view of their various therapeutic effects, namely anti-oxidant, anti-cancer, anti-diabetic, and anti-inflammatory. Since ancient times, turmeric has been extensively used as a naturally occurring traditional Chinese medicine (TCM) and as a crucial home remedy for treatment of many health problems such as inflammation and pain. However, the action of anti-gouty arthritis and analgesic activity of its constituents, especially BDMC, have not been scientifically reported yet. Despite the various bioactivities of BDMC, its utilization in clinics has been restricted owing to poor solubility and bioavailability. This research aimed at enhancing oral bioavailability and pharmacokinetic of BDMC via liposome formulation which was modified with d -α-tocopherol polyethylene glycol 1000 succinate (TPGS or vitamin E TPGS) and was characterized using various parameters both in vitro and in vivo. Also, the analgesic and anti-gouty activities of BDMC-loaded TPGS-modified liposomes were investigated by assessing pain threshold and serum uric acid level in potassium oxonate–induced hyperuricemic rats. As the result, BDMC-loaded TPGS-modified liposomes were formulated successfully with optimal physiochemical properties such as 75.98 ± 5.46 nm (particle size), 0.246 ± 0.011 (polydispersity index), − 38.21 ± 0.29 mV (zeta potential), 96.98 ± 0.17 (encapsulation efficiency (EE%)), and 4.84 ± 0.0089 (drug loading capacity (DL%)). Moreover, the oral bioavailability of liposomized BDMC was approximately 10-fold greater than free BDMC in vivo and in vitro cumulative release rates in pH 1.2 (78%) and pH 7 (68%) respectively. This significantly improved the sustaining drug effect of BDMC compared with free form. In addition, BDMC-TPGS liposome acted as a xanthine oxidase inhibitor to evidently reduce uric acid level in potassium oxonate–induced hyperuricemic rats and remarkably increase pain threshold capacity and reaction time in hot plate test at a dose-dependent manner. Altogether, BDMC-conjugated TPGS liposome could act as favorable nanocarriers against gouty arthritis and intense pain with prospect of overcoming the limitation of BDMC application.
✓ 已验证
DOI
高影响因子
ACS Applied Electronic Materials
·
2025
·
IF 4.7
Ziheng Luo,Shuai Zhang,Genshui Wang,Hengchang Nie,Shiguang Yan,Zhiyan Guo,Yuou Yuan,Liang Shi
Among typical ferroelectric materials, Bi0.5Na0.5TiO3 (BNT) has attracted significant research attention owing to its high intrinsic polarization and polymorphic phase transitions. However, the practical application of pure BNT in high-performance energy storage devices has been limited by its large remnant polarization (Pr) and broad P–E hysteresis loops. Previous studies on element-doped BNT-based materials for enhanced energy storage performance have primarily focused on improving dielectric breakdown strength (Eb) and slimming P–E loops, which typically leads to deteriorated ferroelectric properties (reduced Pmax). To overcome the above problems, this study investigates (0.94–x)Bi0.5Na0.5TiO3-0.06BaTiO3-xMgZrO3:1 mol % Mn (BNT-BT-xMZ:Mn, x = 0, 0.03, 0.06, 0.09, 0.12) thin films. MZ doping induces lattice expansion within the material, where larger domain sizes enhance polarization behavior, leading to increased Pmax (76.81 μC/cm2). Concurrently, the incorporation of MZ promotes cationic disorder, strengthening relaxor behavior. This results in nearly unchanged remnant polarization (Pr) but significantly reduced coercive field (Ec). Consequently, a large polarization difference (ΔP = 70.44 μC/cm2) and slim P–E hysteresis loops are achieved, and synergistic optimization of ferroelectric properties and relaxor characteristics enables the thin film to achieve superior energy storage performance under moderate electric fields (∼2250 kV/cm), ultimately achieving optimal comprehensive performance at x = 0.09, with a recoverable energy density (Wrec) of 55.76 J/cm3 and efficiency (η) of 79.46%. In contrast to previous unilateral approaches solely pursuing high Eb, the synergistic optimization strategy for polarization intensity and P–E hysteresis loop shape provides insights and design principles for modifying thin films as energy storage capacitors.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2013
·
IF 2.417
Jian Xu,Yan-Yan Jia,Shao-Rui Chen,Jian-Tao Ye,Xian-Zhang Bu,Yun Hu,Yun-Zi Ma,Jin-Lei Guo,Pei-Qing Liu
Flavonoids are a class of compounds that exist in nature with the structure of 2-phenyl-chromone. In Chinese traditional medicine , herbal drugs containing flavonoids are widely used for the treatment of inflammation, cardiovascular disease, tumor and so on. In this study, we investigated the anti-inflammatory effect and related mechanisms of a novel synthetic flavonoid, (E)-1-(4-ethoxyphenyl)-3-(4-nitrophenyl)-prop-2-en-1-one (ETH) in lipopolysaccharide (LPS) stimulated macrophages. Our results showed that ETH inhibited LPS-induced TNF-α and IL-6 release in a dose-dependent manner, and decreased TNF-α, IL-1β, IL-6 and iNOS mRNA production. LPS-induced expression of cyclooxygenase-2 (COX-2) was also significantly attenuated by ETH. Pretreatment with ETH reduced the I-κBα phosphorylation, p65 nuclear translocation as well as NF-κB-dependent transcriptional activity. In addition, ETH exhibited a significant protection against LPS-induced inflammatory mortality in mice. Taken together, these findings suggest that ETH can inhibit LPS-induced inflammation via suppressing NF-κB signaling pathway , indicating that ETH may be a potential anti-inflammatory agent.
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Lei Wang,Mengqing Luo,Zihao Zhang,Dan Ji,Xiaohua Chang,Yutian Zhu
Ionic conductive hydrogels as electronic skins (e-skins) have showcased pivotal potentials in the realm of human health monitoring and human–machine interfaces. However, developing intelligent hydrogel with remarkable stretchability and mechanical elasticity is yet challenging. Herein, high-performance ion-conducting hydrogels with unprecedented mechanical properties and good transparent, conductive, self-healing properties are constructed via free-radical polymerization of acrylic acid (AA) within the polyvinyl alcohol (PVA) network, and the subsequent freeze–thaw (F-T) treatment and Zr 4+ ion immersion crosslinking technique. Profiting from the synergy of multiple intermolecular/intramolecular hydrogen bonds between different polymers and coordination bonds of carboxyl-Zr 4+ , the resultant PVA/PAA/Zr 4+ hydrogel exhibits high transparency (∼97 %), superior conductivity (0.6089 S/m), a long elongation at break (2053 %), robust self-healing efficiency (96.3 %), high deformation-tolerate property, and notable mechanical repeatability. These multifaceted attributes render the hydrogel to serve as an ionic conductor for capacitive/resistive −type strain sensor. The hydrogel-based resistive-type strain sensor features a wide detection range (0.5 %-800 %), rapid response time (150 ms), long-term stability, and consistent output stability, making it promising in monitoring diverse human motions and seamless human–machine interactions. Additionally, the hydrogel sensor demonstrates excellent linear response in ultra-wide range (0–900 % strain), high sensitivity, and excellent cycling stability in a capacitive mode, enabling to be used for accurately detecting the weights of objects. As such, this work advances the development of ionic conductive hydrogel as flexible and wearable electronic devices.
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yiye Luo,Lusi Ouyang,Shuo Zhang,Yusheng Wang,Pingzhang Yao,Yin Liu,Xiaoyu Chen,Lin Yuan,Zhiyong Wu
This study investigated volatile organic compound (VOC) variations across commercial grades (G1, G2, G3) of Chinese black truffles ( Tuber indicum ) using electronic nose ( E -nose), headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPM E -GC–MS), and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). E-nose analysis revealed significant differences in sulfur-containing and terpenic compounds between grades. HS-SPME-GC–MS identified 95 VOCs, with G1 exhibiting the highest diversity (75 compounds) and unique markers like 2-butanone. Orthogonal partial least squares-discriminant analysis (OPLS-DA) highlighted 86 discriminatory VOCs (VIP > 1). Odor activity value (OAV) analysis showed G1 had the strongest aroma intensity, driven by esters (ethyl isobutyrate, OAV = 6574) and alcohols (1-octen-3-ol, OAV = 3444). Higher-grade truffles (G1) contained distinct sulfur compounds absent in lower grades. This multi-technique approach provides a biochemical basis for refining truffle grading standards beyond morphological criteria.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL MONITORING AND ASSESSMENT
·
2024
·Vol. 196
·期 12
·1152
·
IF 2.9
Alamgir Aamir,Fatima Noor
In the present study, piped water samples from Gulshan-e-Iqbal Town, Karachi, were evaluated for their Health Risk Assessment (HRA) and Water Quality Index (WQI). For this, different physicochemical and microbiological parameters were analyzed, and the results were evaluated according to the guidelines established by the WHO (2011) and the National Standards for Drinking Water Quality (NSDWQ) (2008). With the exception of sulphate, all physicochemical parameters were well within the guideline values. The mean concentrations of the metals in the samples were in the following order: Ca 2+ > Mg 2+ > Zn > Fe > Pb > Ni > Cr > As. More than 70% of the samples tested for Total Coliform Count (TCC), Total Faecal Coliform Count (TFC), Total Feacal Streptococci (TFS), and Total Aerobic Count (TAC) were feacal contaminated. E. coli was also isolated in almost 84.61% of the tested piped water samples. Results from the WQI revealed that 95.6% of samples had good physico-chemical characteristics, and 26% of the piped water samples had good microbiological quality. The WQI readings of all the samples for metals showed that they were unfit for human consumption. The mean Chronic Daily Intake (CDI) and Hazard Quotient (HQ) values, respectively, were in the following order: Zn > Fe > Pb > Ni > Cr > As, and Pb > As > Ni > Cr > Zn > Fe. All HQ values for As and Pb were greater than 1, and 50% of piped water samples had an HQ value for Ni that was greater than 1, indicating that people may suffer serious health issues as a result. All of the piped water samples except the area of block 13 D (S-5), had HQ values of less than 1 in relation to iron, but Zn HQ values of less than 1 indicated only mild health problems. Discharge of untreated sewage and cross-contamination are all potential sources of contamination that could result in diseases that are harmful to the public’s health. Water monitoring and management projects should be implemented in order to improve pipeline infrastructure and reduce sewage leakages.
✓ 已验证
DOI
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Ran Tao,Chengzhang Wang,Yin Lu,Changwei Zhang,Hao Zhou,Hongxia Chen,WenJun Li
Background This study demonstrated an innovative formulation including the polyprenol (GBP) lipid and vitamin E-TPGS hybrid nanoparticles (NPs) which was aimed to control the transfer of betulinic acid (BA) and low-substituted hydroxyl fullerenol (C60(OH)n). Additionally, it developed BA-C60(OH)n-GBP-TPGS-NPs delivery system and researched the anti-hepatocellular carcinoma (HCC) effects.Materials and Methods The NPs were prepared by nanoprecipitation with ultrasonic-assisted emulsification (UAE) method. It was characterized by scanning electronic microscopy (SEM), transmission electron microscopy (TEM), FTIR spectrum, size distribution and zeta potential. Physical and chemical properties were evaluated through measurement of drug release, stability studies, drug loading efficiency (DE) and encapsulation efficiency (EE). Biological activities were evaluated through measurement of MTT assay, lactate dehydrogenase leakage assay (LDH), cell proliferation assays, cell apoptosis analysis, comet assay, wound healing assay, cell invasion and Western blot analysis.Results and Conclusions The NPs exhibited clear distribution characteristics, improved solubility and stability. BA and C60(OH)n for the NPs displayed a biphasic release pattern with sustained drug release properties. The mixture of C60(OH)n with different hydroxyl groups may have a certain effect on the stability of the NPs system itself. The NPs could effectively inhibit MHCC97H cell proliferation, migration and invasion in vitro. Combined use of C60(OH)n and BA in GBP lipids may improve the inhibit effect of C60(OH)n or BA against HCC cells and reduce cytotoxicity and genotoxicity of C60(OH)n for normal cells. We concluded that one of the important mechanisms of BA-C60(OH)n-GBP-TPGS-NPs inhibiting MHCC97H cells is achieved by up-regulating the expression of Caspase-3, Caspase-8 and Caspase-9.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Energy
·
2023
·
IF 17.6
Zhongxue Bai,Xuechuan Wang,Mengchen Huang,Jiajun Li,Siwei Sun,Xiaoliang Zou,Long Xie,Xiao Wang,Pengbo Xue,Yuyu Feng,Peiyao Huo,Ouyang Yue,Xinhua Liu
The rapid advancement of bio-integrated electronics in recent years has been paralleled by increased interest in the research of stretchable, elastic, conductive, and multi-dimensional sensing electronic skin (e-skin) due to its potential integration with electronic devices and soft tissues. In this work, we presented the fabrication of a nature-skin derived e-skin, denoted as S-P/G@PU e-skin, achieved through an on-demand "top-down" strategy and a triple structure design, employing integrated in-situ polymerization, impregnation, and encapsulation techniques. The collagen fibers weaved hierarchical 3D structure of natural skin, was strategically employed to host conductive polypyrrole and combined with a binary solvent system of glycerin and water. Subsequently, this composite natural skin was coated with polyurethane films, resulting in the engineering of the S-P/G@PU e-skin. This innovative e-skin exhibited remarkable properties, including high tensile strength (8.76 MPa), excellent elasticity (0–180 %), anti-freezing capacity, moisture retention, substantial conductivity (6.3 S/m), and outstanding multi-dimensional sensing capabilities. Importantly, the S-P/G@PU e-skin functioned as both a slow adaptive resistance strain sensor and a fast adaptive single-electrode triboelectric nanogenerator , making it a versatile sensing system that enabled real-time monitoring of various physiological signals from the human body and wide-frequency vibration signals from devices such as cellphones and motors. This study serves as a proof of concept for transforming nature-skin into e-skin, showcasing the potential for integrated wearable electronics , artificial intelligence, and human-machine interfaces.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Runming Wang,Gang Cheng,Zan Dai,Jie Ding,Yunling Liu,Rong Chen
A general ionic-assisted microwave-ultrasonic combined synthetic strategy was developed to fabricate Bi 2 E 3 (E = S, Se, and Te) hierarchitectures. To understand the crystal phase transformation and morphological evolution of Bi 2 S 3 hierarchical nanostructures, the fabrication of Bi 2 S 3 hierarchitectures was investigated by varying sulfidation time and chlorination time. A topotactic transformation from BiOCl microsphere to Bi 2 S 3 hierarchitectures was proposed. It was found that the structure, size, and morphology of Bi 2 S 3 hierarchitectures could be delicately engineered by varying chlorination time, alkyl chain length, and concentration of ionic liquid. Moreover, the as-prepared Bi 2 S 3 hierarchitectures displayed superior capacity of Cr(VI) photoreduction to P25 and irregular Bi 2 S 3 nanostructures under visible light irradiation. Effects of morphology, pH value of reaction system, Cr(VI) concentration, and catalyst dosage on the Cr(VI) photoreduction capacity of Bi 2 S 3 hierarchitectures were also discussed. The enhancement of photoreduction capacity is not only attributed to the intrinsic good electron transfer ability of Bi 2 S 3 , but also to synergetic effects of special architectures, wide photoresponse range, high light photoadsorption, high BET surface area and good electron-hole separation performance. The systematic condition experiments and Cr(VI) photoreduction in electroplating and tannery wastewater experiments further demonstrated that the hierarchical Bi 2 S 3 nanospheres can be used in the actual Cr(VI)-containing wastewater treatment.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Shuwen Zheng,Chenhao Cong,Fuhao Jiang,Shoukun Yu,Bingxiang Li,Fenglin Sun,Xianbin Zheng,Binxuan Diao,Sang Woo Joo,Rong Li,Se Hyun Kim,Xinlin Li,Zhijun Li
In recent years, significant advancements in printed electronics and flexible materials have catalyzed the development of electronic skins for wearable applications. However, the low glass transition temperature of flexible substrates poses a challenge as it is incompatible with the high-temperature annealing required for electrode fabrication, thereby limiting the performance of flexible electronic devices. In this study, we address these limitations by proposing a novel flexible device manufacturing process that combines adhesive printing patterning with a transfer printing technology. By employing poly(vinylidene fluoride) (PVDF)/graphene nitride (GCN) as the transfer substrate and dielectric layer, we successfully fabricated a high-performance dual-mode touch sensor on a large scale. The successful development of this dual-mode sensor can be attributed to two key factors: the construction of a robust hydrogen-bonding network between the PVDF/GCN dielectric layer and the carbon electrode and the ability of GCN to restrict the movement of PVDF molecular chains within the dielectric layer. This restriction reduces the overall polarization of the film, enabling the formation of a complete device structure with a highly sensitive edge electric field. The noncontact sensors developed in this study are fully printable into sensor arrays and can be seamlessly integrated with internet of things technology for wearable applications. These sensors exhibit exceptional tactile response and facilitate effective human–machine interactions over extended distances, underscoring their significant potential in fields such as healthcare and artificial intelligence.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）