JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2021
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IF 8.128
Ruyu Wang,Hongji Liu,Xiangfu Meng,Yong Qian,Xingyu Wang,Feng Zhu,Rongrong Nie,Hui Wang
Silver nanoparticles (Ag NPs) have attracted extensive research interest in bioimaging and biosensing due to their unique surface plasmon resonance. However, the potential aggregation and security anxiety of Ag NPs hinder their further application in biomedical field due to their high surface energy and the possible ionization. Here, binary heterogeneous nanocomplexes constructed from silver nanoparticles and carbon nanomaterials (termed as C-Ag NPs) were reported. The C-Ag NPs with multiple yolk structure were synthesized via a one-step solvothermal route using toluene as carbon precursor and dispersant. The hydrophilic functional groups on the carbon layer endowed the C-Ag NPs excellent chemical stability and water-dispersity. Results showed that C-Ag NPs demonstrated excellent safety profile and excellent biocompatibility, which could be used as an intracellular imaging agent. Moreover, the C-Ag NPs responded specifically to hydroxyl radicals and were expected to serve as a flexible sensor to efficiently detect diseases related to the expression of hydroxyl radicals in the future.
研究领域:
纳米医学
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生物成像
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生物传感
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2012
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IF 3.15
Weijuan Huang,Huijinlan Xu,Yue Xue,Rong Huang,Hongbing Deng,Siyi Pan
Negatively charged electrospun cellulose acetate (CA) fibrous mats were modified with multilayers of the positively charged lysozyme (LY)–chitosan (CS)–organic rectorite (OREC) composites and the negatively charged sodium alginate (ALG) via layer-by-layer (LBL) self-assembly technique. The morphology and antibacterial activity of the resultant samples were investigated by regulating the number of deposition bilayers, the composition of coating bilayers and the outermost layer. Field emission scanning electron microscopy images indicated that the average diameter of LY–CS–OREC/ALG film coating was larger than that of LY–CS/ALG film coating. X-ray photoelectron spectroscopy results implied that LY and OREC were successfully deposited on CA fibers. The LBL structured fibrous mats with the addition of OREC could enhance the degree of inhibition against Escherichia coli and Staphylococcus aureus . (LY–CS–OREC/ALG) 10.5 film coating with the best antibacterial activity, selected for preserving pork for 21 days, could extend the shelf-life of pork for about 3 days.
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DOI
高影响因子
CHEMOSPHERE
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2021
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IF 7.086
Ni Tang,Xue Liu,Meng-Ru Jia,Xin-Yao Shi,Jing-Wei Fu,Dong-Xing Guan,Lena Q. Ma
In this study, a two-step functionalizing strategy by combining co-condensation with grafting procedures was employed to synthesize well-ordered Amino- and Thiol-Bifunctionalized SBA-15 (ATBS) mesoporous silica. Its physicochemical properties , performance, and mechanisms in immobilization of toxic metals Pb and Cd in water and soil were investigated. After bi-functionalization, X-ray diffractometer , transmission electron microscope , and N 2 adsorption-desorption measurements confirmed that the ATBS maintained a highly-ordered mesoporous structure, large surface area and pore volume. The elemental analysis, Fourier transform infrared spectroscopy and X-ray Photoelectron Spectroscopy (XPS) evidenced the successful incorporation of amine and thiol groups into ATBS. These structure and functional characteristics of ATBS benefited Pb and Cd sorption . Sorption isotherms of Pb and Cd were better fit with Sips and Redlich-Peterson models. Sorption kinetics suggested that Pb sorption was mainly regulated by chemical reactions, whereas both diffusion process and chemical reactions were rate-regulating steps in Cd sorption. ATBS showed the maximum sorption capacities for Pb and Cd at 120 and 38 mg g −1 , respectively. The sorption mechanisms revealed by XPS measurements suggested that Cd sorption was mainly attributed to thiol groups while Pb was efficiently bond to both thiol and amino groups. High and stable sorption efficiencies were attained in the pH range of 4–6, with a higher affinity towards Pb than Cd. Furthermore, its ability to immobilize Pb and Cd in soils was examined with an incubation experiment, which showed that ATBS reduced 30–56% of MgCl 2 -extractable Pb and Cd in a contaminated soil. The synthesized sorbent via the two-step functionalizing strategy shows high sorption efficiency towards Pb and Cd, and thus it has potential application in remediating Pb and Cd contaminated water and soils.
研究领域:
环境科学
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材料科学
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化学工程
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DOI
高影响因子
INORGANIC CHEMISTRY
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2014
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IF 4.794
Jun He,Jingjing Duan,Huatian Shi,Jian Huang,Jiahong Huang,Lin Yu,Matthias Zeller,Allen D. Hunter,Zhengtao Xu
The major discovery here is a robust and water-stable metal–organic framework (MOF) material capable of reversible binding of the volatile and reactive molecules of ICl and I2. The immobilization of I2 and ICl, as well as their controllable release thus achieved, is to facilitate the wide-ranging applications of these volatile species as catalysts and reagents in chemical and industrial processes. The framework material TMBP·CuI (hereafter TCuI) can be conveniently prepared in quantitative yields by heating CuI and the organic linker TMBP (3,3′,5,5′-tetramethyl-4,4′-bipyrazol) in acetonitrile. The microporous three-dimensional net of TCuI features CuI chains that contribute to efficient and reversible binding of ICl and I2 molecules, to result in the stoichiometrically well-defined adducts of TCuI·ICl and TCuI·I2, respectively. Moreover, the confinement of a volatile compound like ICl within the MOF medium provides unique opportunities to enhance its reactivity and selectivity as a chemical reagent, as is exemplified by the iodination reactions examined herein. With this exemplary study, we intend to stimulate interest in further exploring MOFs and other porous media (e.g., porous polymers) for entrapping ICl and other volatile reagents (e.g., Br2, SCl2, S2Cl2, and SOCl2) and for potentially novel reactivity associated with the porous medium.
研究领域:
材料科学
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化学工程
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催化化学
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2022
·Vol. 194
·期 11
·5167-5184
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IF 3.094
An Peide,Zhu Fenfen,Liu Shiji,Zhou Xiaolin,Wang Chunfang,Liu Yufeng,Meng Hao,Zhang Xia
Recently, metal–organic frameworks (MOFs) are considered to be the moderate hosts for the bio-enzymes owing to their unique 3D pores and controllable surface affinity to the target molecules. In this work, the benzoic acid (BA)–modulated UiO-66-NO 2 was introduced, and cytochrome c (Cyt C) was chosen as the target enzyme to evaluate the immobilization efficiency of the resulting UiO-66-NO 2 -BA. The immobilization conditions including pH, adsorption time, and temperature and the initial concentrations of BA were optimized. The adsorption kinetics and thermodynamics were analyzed to further explore the enhanced adsorption mechanism. It is worth noted that all the UiO-66-NO 2 -BA exhibited evidently enhanced adsorption capacities in comparison with the unmodified UiO-66-NO 2 due to the formation of the chemical bonds between the UiO-66-NO 2 -BA and cytochrome C, indicating the positive roles of BA modification. Finally, the activities of the immobilized cytochrome C were assessed by using the catalytic oxidation of ABTS in the presence of H 2 O 2 , which reactions were also conducted over the free cytochrome C for comparison. The evidently improved stability under definite pH range, prolonged durability against the organic solvents, and the good reusability of the immobilized cytochrome C highlight the prospect applications of functional MOF immobilized enzymes in the practical catalytic reactions. Graphical abstract
研究领域:
生物化学
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材料科学
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酶工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2019
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IF 8.456
Yindian Wang,Yuan Qi,Chuxuan Chen,Changwen Zhao,Yuhong Ma,Wantai Yang
Exploring a suitable immobilization strategy to improve catalytic efficiency and reusability of cellulase is of great importance to lowering the cost and promoting the industrialization of cellulose-derived bioethanol. In this work, a layered structure with a thin PEG hydrogel as the inner layer and sodium polyacrylate (PAANa) brush as the outer layer was fabricated on low density polyethylene (LDPE) film by visible-light-induced graft polymerization. Two enzymes, β-glucosidase (BG) and cellulase, were separately coimmobilized onto this hierarchical film. As supplementary to cellulase for improving catalytic efficiency, BG was in situ entrapped into the inner PEG hydrogel layer during the graft polymerization from the LDPE surface. After graft polymerization of sodium acrylate on the PEG hydrogel layer was reinitiated, cellulase was covalently attached on the outer PAANa brush layer. Owing to the mild reaction condition (visible-light irradiation and room temperature), the immobilized BG could retain a high activity after the graft polymerization. The immobilization did not alter the optimal pH and temperature of BG or the optimal temperature of cellulase. However, the optimal pH of cellulase shifts to 5.0 after immobilization. Compared with the original activity of single cellulase system and isolated BG/cellulase immobilization system, the dual-enzyme system exhibited 82% and 20% increase in catalytic activity, respectively. The dual-enzyme system could maintain 93% of carboxymethylcellulose sodium salt (CMC) activity after repeating 10 cycles of hydrolysis and 89% of filter paper activity after 6 cycles relative to original activity, exhibiting excellent reusability. This layer coimmobilization system of BG and cellulase on the polymer film displays tremendous potential for practical application in a biorefinery.
研究领域:
生物技术
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材料科学
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酶工程
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DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2018
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IF 3.141
Junhui Rong,Juan Han,Yang Zhou,Lei Wang,Chunmei Li,Yun Wang
In enzymatic conversion of biomass, how to degrade cellulose into fermentable glucose in an economic, efficient, and clean way has become an important subject. As for the application of cellulase in cellulose degradation, the process optimization in enzyme engineering is urgently desired. The traditional multistep purification processes lead to rising production costs and reduced activity of cellulase; meanwhile, the difficulty in reusability of cellulase has also become a big baffle in the cost-effective application of cellulase in biomass degradation. In this paper, the biocatalyst Glu-linker-ELP-GB (GLEGB) containing binary tags, elastin-like polypeptide (ELP), and graphene-binding (GB), was constructed to simplify the purification and immobilization of β-glucosidase (Glu) from Coptotermes formosanus. A high recovery rate (97.2%) and purification fold (18.7) of GLEGB was obtained by only one round of inverse transition cycling (ITC) with 0.5 M (NH4)2SO4 at 25 °C in a short incubating time of 10 min. The purification performance of the one-round ITC method is superior to the commonly used Ni-NTA resin affinity method. Furthermore, the high loading amounts of GLEGB immobilized on GO (698.2 mg g–1) and C3N4 (527.3 mg g–1) were achieved by the synergistic effects of ELP and GB tags. The storage stability and thermal stability of GLEGB was significantly enhanced after immobilization. The recombinant GLEGB immobilized on GO, MGO, graphite, C3N4, C200, and C400 retained 71.4%, 69.5%, 75.1%, 61.2%, 73.5%, and 80.2% of their initial activities respectively after eight cycles. It is worth mentioning that the Km values of GLEGB immobilized on lamellar carbon materials including GO, MGO, and C3N4 are very close to free GLEGB, showing a high affinity of recombinant GLEGB to substrate. To our knowledge, this is the first report on enzyme-linker-ELP-GB system with wide application prospect in the efficient purification and immobilization of enzyme, which can achieve the goal of reducing cost and improving efficiency of biocatalyst in enzymatic conversion of biomass.
研究领域:
生物技术
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酶工程
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生物质转化
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材料科学
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DOI
高影响因子
RENEWABLE ENERGY
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2023
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IF 8.7
Zhiqiang Zou,Hao Zhou,Lingmei Dai,Dehua Liu,Wei Du
The poor stability of metal-organic frameworks (MOFs) limits their practical applications in many fields. Zeolitic imidazolate frameworks (ZIF-8) has been widely studied as an enzyme immobilization carrier. In this paper, the stability of ZIF-8-immobilized lipase in methanolysis for biodiesel production was studied. Fatty acids as conventional substances involved in lipase-mediated natural acylation were found to destroy the structure of ZIF-8 through ligand exchange mechanism. A new MOF was successfully constructed by using phthalic acid (pKa much smaller than 2-methylimidazole) as the exchange ligand. This novel MOF (abbreviated as LeZIF-8-PA X, X represents reaction hours) had dual stability in water and even in pure oleic acid. Lipase immobilized on LeZIF-8-PA 0.5 showed higher specific activity. FTIR analysis showed that enzyme molecule immobilized on Le-ZIF-8-PA 0.5 had higher conformational flexibility than that with ZIF-8 as the carrier. When applied to methanolysis for biodiesel production, ET 2.0/LeZIF-8-PA 0.5 maintained intact morphology while ET 2.0/ZIF-8 almost lost its original morphology, with enzyme activity of 81.5% and 60.2% retained respectively after 5 batch reactions . This work is anticipated to provide new ideas for constructing stable MOFs and promoting the application of MOFs-immobilized enzymes in many chemical and pharmaceutical industrial processes.
研究领域:
材料科学
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化学工程
·
生物化学
·
生物技术
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Chao Huang,Yue Feng,Gopal Patel,Xiao-qian Xu,Jun Qian,Qun Liu,Guo-yin Kai
Beta-glucosidase (EC 3.2.1.21) catalyzes the hydrolysis of cellobiose and cellooligosaccharides containing (1 → 4)-beta-glycosidic bonds to glucose, which is crucial in cellulosic ethanol production. In this study, Aspergillus versicolor , a novel highly productive beta-glucosidase strain, was first isolated from Camptotheca acuminata seeds. The highest beta-glucosidase activity with 812.86 U/mL was obtained by using the response surface methodology, and a 14.4-fold has increased compared to the control. The beta-glucosidase was then purified to homogeneity with recovery yield and specific activity of 25.98% and 499.15 U/mg, respectively. To enhance its stability and recyclability, the purified beta-glucosidase was first immobilized onto magnetic MnO 2 by electrostatic adsorption. The immobilized materials were characterized by FR-IT, TEM and FE-SEM. Compared with the free beta-glucosidase, the immobilized enzyme exhibited enhanced thermal stability (1.5-fold raise in half-life at 50 °C), and reusability (holding over 60% activity after eight cycles), besides, the optimum pH has increased to 6.0. Substrate specificity research suggested that the enzyme had high hydrolytic activity on cellobiose. It also had a hydrolysis effect on (1 → 3) and (1 → 6)-beta-glycosidic linkages. Application trials in cellulose hydrolysis revealed that the immobilized enzyme was comparatively more effective. Our results suggested this novel immobilized beta-glucosidase makes a promising alternative for the cellulosic ethanol production.
研究领域:
生物技术
·
生物化学
·
酶学
·
环境科学
·
可再生能源
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DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2026
·
IF 10.6
Weixi Kong,Biao Li,Qi Zhang,Xuyang Lei,Ke Zheng,Xiaoyang Yue,Yunting Liu,Yanjun Jiang
Chiral amines are key building blocks in pharmaceuticals, yet their efficient and sustainable synthesis via amine dehydrogenase (AmDH) remains challenging due to limitations in substrate loading, enzyme stability, and process scalability. Herein, we report an integrated biocatalytic platform for asymmetric reductive amination (ARA) that synergistically combines a bienzymatic system (CalAmDH for ARA and GDH for cofactor regeneration) with green solvent engineering, enzyme co-immobilization, and continuous-flow technology. A deep eutectic solvent (DES) buffer system based on betaine and acetic acid (Bet–HAc) was designed to enable high substrate concentrations, with molecular dynamics simulations revealing that the DES enhances CalAmDH activity and stability by suppressing an unfavorable helix-to-loop transition. Co-immobilization yielded a robust catalyst that, in a continuous-flow reactor, achieved high conversion, excellent enantioselectivity (>99% ee), and a space-time yield (STY) of 49.11 g·L⁻¹·h⁻¹. This represents a 185-fold enhancement over prior reports and demonstrates the power of combining DES-mediated stabilization with process intensification for the green, scalable synthesis of chiral amines.
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Simin Zhang,Xiangfeng Chen,Yaping Shi,Xiangyu Zhu,Zongwei Cai
As bioactive compounds, chiral amino acids (AAs) have been demonstrated to play pivotal roles in different physiological processes, thereby influencing human health. The assessment of the relative enantiomeric ratio through analytical methods is essential for indicating the abnormal status of disease progression, which drives the development of effective methods for resolving and identifying enantiomers. This work presents a robust strategy for the ultrahigh-resolution analysis of chiral AAs using MALDI-TIMS-MS. By employing a chiral derivatization process using S-NIFE and stabilizing the soft chain of the derivatization reagent with bimetal ion addition, the separation of 19 pairs of proteinogenic AAs was successfully achieved. This method not only demonstrates outstanding separation capability but also proves its efficiency in determining enantiomeric ratios, extending its applicability to real sample analysis.
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Qiang Niu,Gelimei Zhang,Tao Yang,Qiyuan Chen,Fenghui Wu,Dandan Chen,Yan Guo,Ding Yuan,Yuanchuan Ren,Xuejun Zhu
Synthesized MOF-based carbon sponge materials through electrostatic self-assembly. • The removal efficiency of this material for Hg 0 surpasses traditional limits. • Over 90% removal efficiency for Hg 0 in simulated actual flue gas after 4 cycles. Mercury emissions from coal combustion present persistent risks to both public health and ecosystem integrity. Although powdered adsorbents exhibit high gaseous elemental mercury (Hg 0 ) removal efficiency, their practical deployment is often hindered by particle agglomeration, poor recoverability, and entrainment in flue gas streams. Herein, this article reports a structured monolithic adsorbent CuS-BTC@CMS, fabricated by electrostatic self-assembly of CuS derived from Cu-BTC (BTC = 1,3,5-benzenetricarboxylic acid) onto a carbonized melamine sponge (CMS). Leveraging the spatial confinement effect of the metal organic framework (MOF) precursor, the resulting CuS-BTC demonstrates superior Hg 0 adsorption performance at elevated temperatures (150 °C), achieving an average removal efficiency of 95.91% over 4 h. Mechanistic investigations reveal that polysulfide species (S x 2− ), particularly long-chain variants, serve as the predominant active sites for Hg 0 capture. Through electrostatic interactions, negatively charged CuS-BTC is uniformly assembled onto the positively charged CMS surface, which was pre-functionalized with CTAB (cetyltrimethylammonium bromide), ensuring high dispersion of active components. The macroporous architecture of the support, combined with the well-dispersed CuS-BTC, facilitates efficient Hg 0 transport to reactive sites, enabling CuS-BTC@CMS to achieve an average Hg 0 removal efficiency of 97.4% over 4 h, outperforming the parent Cu-BTC. The monolithic adsorbent maintains excellent Hg 0 removal performance across a wide temperature range (30–180 °C) and exhibits robust tolerance to diverse flue gas components (SO 2 , NO, O 2 CO 2 , and H 2 O), underscoring its adaptability to variable operating conditions. Moreover, it demonstrates favorable regenerability, retaining over 90% Hg 0 removal efficiency after four regeneration cycles. The maximum Hg 0 adsorption capacity of CuS-BTC@CMS reaches 11.87 mg/g with the breakthrough ratio of 99.97%, corresponding to 108.41 mg/g when normalized to the CuS-BTC loading. Download: Download high-res image (116KB) Download: Download full-size image
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产品关联
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高影响因子
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