INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Tongchao Su,Youtong Zhang,Wenkai Du,Jie Zeng,Haiyan Gao
The trehalose (TL) was modified with sodium alginate, starch, and the effects of modified trehalose particle on the gluten protein and steamed bread properties were investigated. The results showed that for dough frozen after 30 d, the addition of 0.8 % modified TL significantly increased water-holding capacity by 12.12 %, improved the freezing rate, and the change of binding water could be better controlled. Besides, the α-helix content and random coil content of gluten protein was increased and decreased after adding modified TL, respectively, improving the ordered structure of wheat gluten protein and maintaining its aggregation characteristics and structural stability to some extent. Meanwhile, the brightness, water-holding capacity and specific volume of steamed bread was increased after adding 0.8 % modified TL, which improved and maintained the normal texture and internal structure of steamed bread. The results can provide a basis for the application of trehalose in flour products.
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JOURNAL OF FLUORESCENCE
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2024
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IF 2.6
Chen Xian,Zhang Menghan,Zheng Lang,Deng Xiaoqin,Chen Qiang,Han Wendi,Huang Zhengjun,Weng Shaohuang
The establishment of a convenient and efficient testing method is crucial and needed for the monitoring of diosmin. In this study, nitrogen doped carbon dots (N-CDs) with the particle size distribution of 2.5–5.7 nm and the average diameter of 4.1 nm were successfully synthesized using a simple strategy. N-CDs exhibited excellent and stable fluorescence performance with the quantum yield of 22.33%. Correspondingly, a fluorescence analytical method was developed for diosmin determination using N-CDs as probe. UV-vis absorbance spectroscopy and the evaluation of internal filtering parameters verified that the mechanism causing the quenching of N-CDs was an internal filtering effect (IFE). The concentration of diosmin can be directly evaluated based on the quenched fluorescence intensities. After optimizing experimental conditions, it was found that the fluorescence quenching efficiency ((F 0 -F)/F 0 ) of N-CDs exhibited a good linear relationship with the concentration of diosmin (C Diosmin ) in the range of 3.0–50 µg/mL. The limit of detection (LOD) was 0.86 µg/mL based on 3σ/slope ( n = 13). This method was successfully applied to accurately determine the content of diosmin in diosmin tablets and human plasma samples with good reproducibility. It stands out for its simplicity, speed, and acceptable determination performance.
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LANGMUIR
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2024
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IF 3.7
Man Li,Min Luo,Xiaoxiao Niu,Angsen Sheng,Zhen Wang,Xiaoqing He,Huaiyu Bu,Gang Xie
Synthesis methods of carbon nanomaterials have been developed vigorously in recent years, among which a simple, green, and mature approach is of more research significance. Carbon nanomaterials have depicted an impact on the growth and development of plants. In this study, a new type of carbon nanomaterial, superhydrophilic carbon aerogel (CA), was synthesized via a hydrothermal process using carbohydrates and water-soluble polymers as raw materials. Characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, powder X-ray diffraction, and N2 adsorption analysis, the exemplified CA presented to have porous three-dimensional network structure composed of individual particles with diameters of 25 nm, with reactive surface, single composition, high specific surface area (89.94 m2·g–1), and wide range of density variation. The 9 mg·mL-1 CA suspension had a significant positive effect on the root growth of wheat seedlings, with promoted root elongation (about 67.17% longer) and root diameter (about 28.95% thicker) compared with those of the control group. The cytological results suggested that CA treatment triggered the propagation of meristematic cells, and the increased number of meristematic cells (65.79% more than the control group) led to enhanced root growth by upregulated expression of related phytohormone genes in wheat seedlings.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Jing Li,Jijie Shi,Hui Ren,Junjie Yang,Dan Xiao,Xiaoqin Li,Xinhui Wang,Yong Guo,Zhaoyu Jin
A novel non-enzymatic glucose sensor is developed by directly growing flower-shaped NiCo 2 O 4 microsphere on flexible carbon cloth (NCO/CC) through a one-step electro-deposition with subsequent mild heat treatment. Benefiting from the excellent conductivity between the three-dimensional (3D) structure of NiCo 2 O 4 and CC, as well as the synergic effect between the nickel and cobalt cations, the NCO/CC can not only offer more additional active sites, but also significantly enhance its electrocatalytic performance. Under the optimized conditions, the NCO/CC electrode reveals excellent activity with a broad linear detection range from 10 to 2000 μM, a high sensitivity of 5260 μA mM −1 cm −2 , and a low detection limit (LOD) of 0.2 μM, along with good repeatability and long-term stability. Furthermore, the NCO/CC was successfully utilized to detect glucose in actual beverage samples, showing competitive performance. The straightforward and practical method offers a novel approach to measuring glucose in real-world applications. Novelty statement 3D flower-shaped NiCo 2 O 4 microspheres growing on flexible carbon cloth (NCO/CC) were fabricated by a facile one-step electro-deposition with subsequent mild heat treatment. The fabricated NCO/CC electrode exhibits a wide linear detection range, high selectivity, good reproducibility, and stability towards the detection of glucose.
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ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Yonghong Zha,Yansong Li,Jianhua Zhou,Xiaolan Liu,Ki Soo Park,Yu Zhou
Given the harmful effect of pesticide residues, it is essential to develop portable and accurate biosensors for the analysis of pesticides in agricultural products. In this paper, we demonstrated a dual-mode fluorescent/intelligent (DM-f/DM-i) lateral flow immunoassay (LFIA) for chloroacetamide herbicides, which utilized horseradish peroxidase-IgG conjugated time-resolved fluorescent nanoparticle probes as both a signal label and amplification tool. With the newly developed LFIA in the DM-f mode, the limits of detection (LODs) were 0.08 ng/mL of acetochlor, 0.29 ng/mL of metolachlor, 0.51 ng/mL of Propisochlor, and 0.13 ng/mL of their mixture. In the DM-i mode, machine learning (ML) algorithms were used for image segmentation, feature extraction, and correlation analysis to obtain multivariate fitted equations, which had high reliability in the regression model with R2 of 0.95 in the range of 2 × 102–2 × 105 pg/mL. Importantly, the practical applicability was successfully validated by determining chloroacetamide herbicides in the corn sample with good recovery rates (85.4 to 109.3%) that correlate well with the regression model. The newly developed dual-mode LFIA with reduced detection time (12 min) holds great potential for pesticide monitoring in equipment-limited environments using a portable test strip reader and laboratory conditions using ML algorithms.
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Journal of Cleaner Production
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2024
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IF 9.7
Fan Yang,Zexuan Zhang,Jiugang Yuan,Jin Xu,Qiao Ji,Yutong Bai
In recent years, resource optimization has been recognized as a top priority among many challenges. Studies have indicated that valuable products can be created by combining natural or discarded resources with cutting-edge biosynthesis techniques. This approach is considered foundational for establishing a “zero-waste” socio-economic structure. This research focuses on optimizing resources and minimizing environmental impacts, especially within the manufacturing and fashion sectors. An innovative leather-like material named BC-BioLeath (Bacterial Cellulose-Based Bio Leather) has been introduced. BC was produced by the fermentation of coconut water, a byproduct of the food industry. Traditional leather-like processing, including tanning, was applied to BC using food waste such as oxidized edible oil and coffee grounds, along with timber industry by-products like vegetable tannins. Studies involving SEM observation, mechanical, style, and dyeing performance testing revealed that the product, BC-BioLeath, exhibited superior mechanical properties. For instance, BC crosslinked with oxidized edible oil showed a tensile strength of 82.14 MPa, surpassing traditional cowhide used in shoe uppers by 2.08 times. When plasticized with glycerol, the tensile strength reached 56.19 MPa, with breaking elongation of 36%. Beyond its mechanical prowess, the product also achieved economic and environmental benefits through waste repurposing, contributing to a natural energy cycle. BC-BioLeath was identified as a more humane and eco-friendly leather alternative. This method showcased not just efficient waste repurposing but also introduced a novel, microbe-focused sustainable production approach. Such a model offered a genuine circular economy solution for high-polluting sectors, such as the leather fashion industry. A cost analysis showed that BC-BioLeath's production cost of $97.92/m 2 (excluding labor and operating costs: $7.52/m 2 ) was within the commercial leather range of $39.7 to $220.8/m 2 . This indicated its sustainability as a competitive alternative.
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SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Shuai Zhang,Jincheng Xiong,Boyan Sun,Chunchao Yin,Yongjun Zheng,Xingxing Liu,Yanchen Gao,Xiaomei Wang,Sihan Wang,Haiyang Jiang
Emerging contaminants seriously jeopardize food safety and even human health. This study presents the formation of all-in-one aggregation-induced emission (AlE)-type immunomagnetic ZlF-8 (AlMZ) through an ultra-simple self-assembly method in an aqueous phase, successfully harnessing the functions of immuno-recognition, luminescence, and magnetism, Here, we have achieved a dual enhancement in detection efficiency rapidly enriching targets and performing a triple lateral flow immunoassay (LFlA) for ractopamine, diethylstilbestrol, and chloramphenicol within 40 minutes, The dual readout biosensor was then configured to automatically extract the color intensity values of the three test lines using a smartphone. A portable sensing platform ensures stable temperature and light intensity in non-laboratory environments, providing favorable conditions for immunological detection. This study demonstrated low detection limits of 0.11, 0.14, and 0.01ug/L for ractopamine, diethylstilbestrol, and chloramphenicol, respectively, Applications in pork and tap water showed excellent practicality and stability, with recovery rates ranging from 87.2 % to 112.7 %, emphasizing the potential for applications in food and environmental safety.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Weihua Xu,Yanling Liu,Kaiming Fan,Ziyi Yuan,Siqi Wang,Linjun Li,Shengji Xia
The widespread presence of hardness ions in surface water poses potential challenges for drinking water treatment, which can be efficiently removed by nanofiltration (NF) membranes. Compared with mainstream spiral-wound polyamide ones, hollow fiber (HF) NF membranes present a promising alternative due to the potential advantages in operation. This study investigated the ion rejection properties of a commercial polyelectrolyte multilayer (PEM)-based HF NF membrane by treating surface water with varying hardness levels, respectively resembling the raw water hardness of northern and southern areas of China. Results show that compared to NF270 membrane, the PEM HF NF membrane had a thicker separation layer with higher surface hydrophobicity, leading to a lower water permeance. Despite the predominantly negatively charged surface, the PEM HF NF membrane achieved high CaCl 2 and MgCl 2 rejections of 85.4 % and 90.2 %, respectively. During surface water treatment, the PEM HF NF membrane maintained stable water purification performance after several cycles of backwashing and outperformed NF270 membrane in removing organic matters and hardness ions. The distinct ion rejection characteristics and underlying mechanisms for the PEM HF NF membrane treating surface water with different hardness levels were comprehensively elucidated. Moreover, the membrane negative rejection of Na + in low hardness water affected by the Na + /Cl - molar ratio in the feed was particularly interpreted. This study provides performance demonstration and mechanistic insights for promoting the potential application of PEM HF NF membranes for drinking water treatment.
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FOOD CHEMISTRY
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2024
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IF 8.5
Shengxin Ji,Yong Yang,Huipin Li,Zhen Li,Biao Suo,Minghui Fan,Zhilu Ai
This study aimed to enhance the rehydration property of fried-free instant noodles using a fermentation method with exopolysaccharide-producing Weissella confusa , thereby improving their quality. The effect of sourdough fermented with W. confusa on the rehydration property, cooking qualities and in vitro starch digestibility of fried-free instant noodles was investigated. Compared with the control group, the rehydration time of fried-free instant noodles prepared using W. confusa –fermented sourdough supplemented with 2 % sucrose was significantly reduced by 17.36 % from 403 to 333 s, the cooking loss was significantly decreased by 12.72 % from 12.50 % to 10.91 %, and the digestible starch content was significantly increased by 5.40 % from 73.09 % to 77.04 % ( p < 0.05). Sourdough fermentation with W. confusa created an acidic environment and produced hydrophilic exopolysaccharides, which significantly enhanced the rehydration property and cooking quality of fried-free instant noodles by improving their degree of gelatinization and reducing their relative crystallinity ( p < 0.05).
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Food Bioscience
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2024
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IF 4.8
Caiyuan Yu,Yuanwei Sun,Chunkai Yang,Yan Fang,Qi Meng,Yibin Qiu,Peng Lei,Hong Xu,Qi Zhang,Sha Li
Tremella fuciformis , one of the most approved mushrooms worldwide, possesses both edible and medicinal value. However, low transformation efficiency and high false positive rate, long verification period, and low expression of heterologous proteins hinder further genetic research. In this study, a polyethylene glycol-mediated genetic transformation system was established by using the homologous resistance marker carboxin in T. fuciformis . By optimizing the protoplast quality and transformation conditions, the transformation efficiency reached 8.23 CFU/μg, and the positive rate reached 64.95%. Moreover, Driselase-Heating-Freezing screening system was developed for rapid transformants verification, which took only 35–45 min and made the colony PCR 100% accurate. Considering the rationality of tRNA use frequency in heterologous genes, the coordinated relative synonymous codon usage (RSCU) strategy was adopted to recode the heterologous gene sequence, which ensured the protein expression level. This study comprehensively enhances molecular biology tools in T. fuciformis , and lays a foundation for further development.
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IONICS
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2024
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IF 2.4
Qiu Shengzhou,Wu Chenxi,Akter Sima,Hong Shaoli,Liu Huihong,Mahmud Sakil
This study presents the development of copper oxide–modified screen-printed carbon electrodes (CuO/SPCE) for the sensitive electrochemical detection of isoniazid (INZ). The electrodeposition of CuO on the surface of SPCE in a dilute CuSO 4 solution forms CuO amorphisms or clusters, which catalyze the oxidation of INZ. The sensor exhibited a linear response to INZ concentrations ranging from 4 to 200 μM with a correlation coefficient of 0.991 and a limit of detection (LoD) of 8.39 μM. Stability and reproducibility were confirmed through repetitive measurements with relative standard deviations of 0.27% and 1.20%, respectively. Interference studies demonstrated the sensor’s selectivity toward INZ, and a real sample analysis of pharmaceutical tablets yielded recovery rates ranging from 99.50 to 105.03%. Hence, regular monitoring of INZ dosage levels in human body fluids is critical, necessitating the development of sensitive, effective, and consistent methods to quantify INZ in clinical and pharmaceutical samples. Graphical Abstract
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MONATSHEFTE FUR CHEMIE
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2024
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IF 1.7
Qiu Shengzhou,Wu Chenxi,Akter Sima,Hong Shaoli,Liu Huihong,Mahmud Sakil
Addressing the challenges of tuberculosis drug detection, this study investigates the application of copper(II) oxide modified screen-printed carbon electrodes (CuO/SPCEs) for the electrochemical detection of rifampicin, a critical drug in the treatment of tuberculosis and mycobacterial infections. The CuO/SPCE was fabricated via a constant potential electrodeposition technique and characterized using voltammetry. The bare SPCE surface exhibited a rough, porous structure, whereas the electrodeposition of CuO resulted in a smoother CuO/SPCE surface. The sensor exhibited high sensitivity with a detection limit of 2.89 μM for rifampicin and demonstrated excellent stability with a relative standard deviation of 3.5% over 10 measurements. The reproducibility was confirmed with an RSD of 4.2% across different electrodes. It also showed a linear response to rifampicin concentrations ranging from 10 to 200 μM. The real sample analysis showed a linear relationship between rifampicin concentration and peak current ( I = 0.739 + 0.008 c ), with a high correlation coefficient ( R 2 = 0.995). This electrochemical sensor’s simplicity, cost-effectiveness, and rapid response make it a promising tool for point-of-care applications in tuberculosis management. Graphical abstract
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危险品化学品经营许可证（带存储）