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INTERNATIONAL JOURNAL OF FOOD PROPERTIES · 2018 · IF 1.845

Seasonal variations of phenolic profiles and antioxidant activity of walnut (Juglans sigillata Dode) green husks

Binbin Shi,Wene Zhang,Xue Li,Xuejun Pan

The phenolics content and antioxidant activity of walnut (Juglans sigillata Dode) green husks were analysed during different stages of development. Contents of total phenolics and total flavonoids decreased from April 25th to June 7th, then increased during June and July, and decreased again at the beginning of August. Thirteen types of phenolic compounds were identified using HPLC of which juglone had the highest content. Because of seasonal variations, the trends of different phenolic compounds were different. Contents of both total phenolics and total flavonoids were strongly correlated with antioxidant activity; however, compared with totals, less significant correlations were observed between individual phenolic compounds and antioxidant activities.

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LWT-FOOD SCIENCE AND TECHNOLOGY · 2018 · IF 3.129

Investigation of inhibition of lipid oxidation by L-carnosine using an oxidized-myoglobin-mediated washed fish muscle system

Shulan Xiao,Hong Zhuang,Guanghong Zhou,Jianhao Zhang

The objective of the present study was to understand mechanisms of inhibitory effect of l -carnosine on lipid oxidation using an oxidized myoglobin (metMb)-mediated washed cod muscle system. Experiments were conducted to compare l -carnosine with commercial antioxidants, tert-Butylhydroquinone (TBHQ) and α-tocopherol, and metal chelating reagent EDTA for inhibition of lipid oxidation, to compare l -carnosine with TBHQ for scavenging free radicals and to evaluate the effect of l -carnosine on structure of pro-oxidant metMb with spectral methods. Results showed that the inhibitory effect of l -carnosine on lipid oxidation was comparable with TBHQ and better than α-tocopherol. However, 1,1-diphenyl-2-picrylhydrazyl assay showed that TBHQ was much more effective in scavenging free radicals than l -carnosine. Comparing with 1.5% EDTA, 1.5% l -carnosine was much more effective against lipid oxidation. Incubation of l -carnosine with metMb resulted in spectral changes of metMb in wavelengths between 370 nm and 450 nm, Soret band shifted from 409 nm to 420 nm, and reduced molar ellepticity values. These results indicate that the high inhibitory effects of l -carnosine on meat lipid oxidation may result from not only its scavenging and chelating functions but also its effect on pro-oxidant metMb structure.

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EUROPEAN FOOD RESEARCH AND TECHNOLOGY · 2018 ·Vol. 244 ·期 11 ·2007-2017 · IF 1.919

Quantification of polyphenol composition and multiple statistical analyses of biological activity in Portuguese red wines

Bai Shuang,Cui Can,Liu Jing,Li Pei,Li Qing,Bi Kaishun

The objective of the research was to evaluate potential biological activities of Portuguese red wines with different grape types on the level of composition characterization. An ultrafast liquid chromatography coupled with triple quadrupole mass spectrometry method was validated and used for qualitative analysis of commercial red wine samples through simultaneously determining 31 major active components. Meanwhile, the antioxidant activities of red wines were measured by DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS [2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] and CUPRAC (cupric ion reducing antioxidant capacity) assay. The relations between polyphenols and antioxidant values were further considered by multivariate statistical analysis. Results of correlation analysis and stepwise multiple regression analysis showed that antioxidant activities were highly correlated to polyphenolic profiles, with significant contributions of procyanidins and phenolic acids. Consequently, the assessment method we established appeared to be an effective tool for appraising the nutritional value of polyphenols in wines, and the research strategy could be applied to the evaluation of other beverages.

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PLoS One · 2018 ·Vol. 13 ·期 6 ·e0198072 · IF 2.766

Effect of different planting areas on the chemical compositions and hypoglycemic and antioxidant activities of mulberry leaf extracts in Southern China

Jing-Yi Hao,Yi Wan,Xiao-Hui Yao,Wei-Guo Zhao,Run-Ze Hu,Cong Chen,Long Li,Dong-Yang Zhang,Guo-Hua Wu

Guangdong, Guangxi and Chongqing are emerging sericulture areas in China where the production of mulberry leaves is huge. In order to identity high quality mulberry leaves that are suitable for healthy products to expand planting, 24 samples from three regions (Guangdong, Guangxi, Chongqing) in the south of China were quantified for two alkaloids (1-deoxynojirimycin and fagomine) and five phenols (chlorogenic acid, rutin, isoquercitrin, etc.) using high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Additionally, the total phenolic and total flavonoid contents, antioxidant and glycosidase inhibitory activities (hypoglycemic activity) were tested using different assays (DPPH, ABTS, FRAP) to comprehensively evaluate the quality of the mulberry leaves. The contents of DNJ and fagomine ranged from 0.401±0.003 to 5.309±0.036 mg/g and from 0.279±0.031 to 2.300±0.060 mg/g, respectively. The main phenolic constituents were chlorogenic acid, rutin and isoquercitrin, with chlorogenic acid present in the highest concentrations, ranging from 3.104±0.191 to 10.050±0.143 mg/g. The antioxidant activity exhibited a tendency as follows: Guangxi > Guangdong > Chongqing, except for two samples from Chongqing, which showed the highest antioxidant activity. Based on our study, mulberry leaves from Guangdong and Guangxi could be future sources of natural hypoglycemic and antioxidant products.

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FOOD CHEMISTRY · 2018 · IF 4.946

Distribution and quantitative analysis of phenolic compounds in fractions of Japonica and Indica rice

Chao Ding,Qiang Liu,Peng Li,Yongsheng Pei,Tingting Tao,Yan Wang,Wei Yan,Guofeng Yang,Xiaolong Shao

Utilization of phenolic compounds in rice husk and bran is important for improving the functionality of rice by-products. Eight rice varieties planted in different area were selected to analyze the phenolic compounds distribution of fractions in Japonica and Indica rice by using UPLC-MS method. A total of 12 phenolic compounds were identified in all rice varieties. Ferulic acid, gallic acid, protocatechuic acid and syringic acid were the dominant phenolic compounds in rice bran, while p-Hydroxybenzaldehyde was the main phenolic compounds existed in rice husk (14.46–23.72 µg·g −1 ). Bran and husk fractions provide more than 90% of phenolic compounds and antioxidant activity of whole rice. Regardless of the planting environmental effects, Japonica rice has significant higher phenolic compounds and antioxidant activity than Indica rice (P < 0.05). Therefore, it can be concluded that the distribution of phenolic compounds were strongly correlated with the rice varieties and fractions.

CAS / MDL: 1898-66-4 149-91-7
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JOURNAL OF MATERIALS SCIENCE · 2019 ·Vol. 54 ·期 16 ·11243-11253 · IF 3.442

In vitro preparation and characterization of amorphous calcium carbonate nanoparticles for applications in curcumin delivery

Rao Chaohui,Guo Xia,Li Min,Sun Xiaoqing,Lian Xiaojie,Wang Huifang,Gao Xianghua,Niu Baolong,Li Wenfeng

The development of high drug loading and sustained release nanoparticles by simple and economical methods is significant for the treatment of diseases. Amorphous calcium carbonate (ACC) is an ideal drug delivery system because of its excellent pH responsiveness, biocompatibility and biodegradability. In this work, ACC nanoparticles were successfully precipitated in ethanol–calcium solutions using ammonium carbonate as the internal source of CO 2 , which exhibited a relatively narrow size distribution in range of 10–200 nm. The microscopic morphology, amorphous characters, porous structure and thermal behavior of resultants were investigated by electron microscopy, Fourier-transform infrared spectroscopy, Brunauer–Emmett–Teller and thermogravimetric analysis, respectively. In vitro drug release assay showed that ACC nanoparticles had high loading capacity of curcumin (Cur) and favorable drug release properties. Furthermore, ACC-Cur showed excellent abilities to scavenge free radicals, protect Cur stability and damage A549 cells, providing a broadened space for the applications of this material in the fields of biomedical or food.

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INTERNATIONAL JOURNAL OF FOOD PROPERTIES · 2019 · IF 1.398

Physicochemical properties and bioactive compounds of fermented pomegranate juice as affected by high-pressure processing and thermal treatment

Yuan Ma,Xiaocui Yin,Xiufang Bi,Fan Su,Zhilin Liang,Ming Luo,Dongxu Fu,Yage Xing,Zhenming Che

High-pressure processing (HPP) is a novel non-thermal processing technology, ensuring the safety of food products, as well as preserving their nutritional and functional characteristics, and avoiding the harmful effects of traditional thermal technologies. This study examined the inactivation of microorganisms and the HPP kinetic model in fermented pomegranate juice (FPJ) at different pressures (400 MPa, 500 MPa, and 600 MPa), and different treatment times (3 min, 5 min, 7 min, and 10 min). Moreover, HPP and thermal processing (TP) (65 °C/20 min) treatment responsible for a similar microbial inactivation activity, were compared by examining their impact on bioactive compounds, volatile compounds, and various quality attributes to provide a theoretical basis for FPJ processing. The inactivation curves of the microorganisms as a result of HPP were fitted using the Weibull model. Furthermore, HPP at or over 600 MPa/3 min inactivated the microorganisms and sufficiently retained the microbial populations investigated in this study below the detection limit. Following HPP and TP treatment, the total color difference (ΔE) values, total soluble solids (TSS), pH and titratable acidity (TA) had not significantly (p>0.05) changed. For total flavonoids, total phenols, anthocyanins, and antioxidant activity, the maximal retention of 91.18%, 97.52%, 95.69%, and 95.89% was achieved at 600 MPa/3 min, which was 4.62%, 24.61%, 26.83%, and 7.08% higher than in the TP-treated sample. The main volatile compounds in FPJ were esters and alcohols, and the FPJ treated at 600 MPa/3 min exhibited the highest alcohol content, while the esters were 2.27% higher than in the control. Considering the pasteurization effect and quality maintenance abilities of HPP and TP treatment, 600 MPa/3 min could be considered as an optimal condition in facilitating FPJ processing.

CAS / MDL: 1898-66-4
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Oxidative Medicine and Cellular Longevity · 2019 · IF 4.868

Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea

Xiang Li,Si Chen,Jing-En Li,Ning Wang,Xin Liu,Qi An,Xi-Mei Ye,Zi-Tong Zhao,Meng Zhao,Yi Han,Ke-Hui Ouyang,Wen-Jun Wang

The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea. The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC). Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied. The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was . Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro. Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens. The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.

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Journal of Food Measurement and Characterization · 2017 ·Vol. 12 ·期 2 ·967-973 · IF 0.536

Phenolic compounds from date pits: ultrasonic-assisted extraction, antioxidant activity and component identification

Liu Yong,Wei Shoulian,Wu Min,Yang Shuqiu

Ultrasonic and response surface methodology were used to extract phenolic compounds from date pits and optimize extraction parameters including ultrasonic time, solid to liquid ratio and ethanol concentration. A mathematical model of the three parameters for a high extraction yield was established. The results showed that all the extraction parameters and the interaction between solid to liquid ratio and ethanol concentration significantly affected the extraction yield. The optimized extraction parameters were ultrasonic time of 42 min, solid to liquid ratio of 1:46 g/mL, and ethanol concentration of 60%, under which the extraction yield was 8.26%. In vitro antioxidant activity and components of the phenolic compounds was investigated by the antioxidant tests and HPLC, respectively. The antioxidant assays of DPPH and ABTS free radicals, reducing power and total antioxidant capacity suggested that the phenolic compounds from date pits had a high antioxidant activity, and the HPLC analyses revealed that the extracts were mainly composed of seven phenolics.

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INDUSTRIAL CROPS AND PRODUCTS · 2017 · IF 3.181

Antioxidant capacities and total phenolic contents of 30 flowers

Guan-Lin Chen,Song-Gen Chen,Yi Xiao,Nan-Lin Fu

In order to find new sources of natural antioxidants, total phenolic content (TPC) and total flavonoid content (TFC) of 30 flowers in their free, esterified and insoluble-bound forms were determined. Rosa rugosa Thunb (pink) showed the highest TPC, and Osmanthus fragrans had the highest TFC. The antioxidant activities of 30 flowers were determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC) assays. Rosa rugosa Thunb (purple) and Rosa rugosa Thunb (pink) had the highest DPPH (612.79 and 544.75   μmol Trolox/g DW), FRAP (273.10 and 301.14   μmol Trolox/g DW) and TEAC (1013.71 and 937.19   μmol Trolox/g DW) value. Furthermore, several phenolic compounds were detected using ultra performance liquid chromatography (UPLC) technique, and (+)-catechin, ferulic acid, isoquercitrin, and quercitrin were widely found in these flowers. The results implied that the flowers were important natural sources of bioactive components with higher antioxidant capacities for use in the food and pharmaceutical industries.

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JOURNAL OF FOOD PROCESSING AND PRESERVATION · 2017 ·Vol. 42 ·期 2 ·e13507 · IF 0.791

Combined effects of pulsed electric field and ultrasound on bioactive compounds and microbial quality of grapefruit juice

Rana Muhammad Aadil,Xin-An Zeng,Zhong Han,Amna Sahar,Anees Ahmed Khalil,Ubaid Ur Rahman,Muneeb Khan,Tariq Mehmood

The combined effect of ultrasound (US) and pulsed electric field (PEF) was investigated on microbial load and bioactive compounds of grapefruit juice. Grapefruit juice was PEF treated (flow rate: 80 ml/min, pulse frequency: 1 kHz, 20 kVcm −1 electric field strength, temperature: 40 °C, time: 600 μs) followed by US treatment in an ultrasonic bath cleaner radiating 600 W at frequency of 28 KHz and 20 °C for 30 min. PEF and US treatment resulted in a significant reduction in microbial load as compared to the control group. Using combined (PEF + US) treatment, carotenoids, lycopene, anthocyanin contents, and total antioxidant activity were increased from 0.84 μg/ml, 0.32 μg/ml, 1.37 mg/L, and 177.48 ascorbic acid equivalent µg/g (control) to 1.26 μg/ml, 0.92 μg/ml, 1.68 mg/L, and 262.32 ascorbic acid equivalent µg/g, respectively. The findings demonstrated that PEF + US could be successfully used for preserving bioactive compounds in grapefruit juice while improving the microbial quality for a better shelf-life. Practical applications In recent years, nonthermal techniques, such as sonication and PEF or combination of these are used for the inactivation of microorganism and preserving the bioactive compounds of different juices. The results of the present study revealed that combined treatment (PEF + US) could improve the antioxidant activity, total phenolics, flavonols, flavonoids, lycopene, and total carotenoids. The findings of this study suggested that PEF + US has a potential to improve the quality of grapefruit juice and may be employed for the processing of grapefruit juice at industrial scale.

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JOURNAL OF CHROMATOGRAPHIC SCIENCE · 2022 ·Vol. 61 ·期 8 ·766-772 · IF 1.555

Simultaneous Determination of Phenylethanoid Glycosides and Antioxidant Activity of Syringa pubescens Turcz. from Different Geographical Origin in China

Yang Qinyu,Wang Xinsheng,Zhang Yanlong,Li Mengyuan,Wang Panxu,Zhang Jiameng,Liu Kun,Wu Yanfang,Shen Guomin,Ma Zhanqiang

A high-performance liquid chromatograph with diode array detector was established for the simultaneous determination of five phenylethanoid glycosides in Syringa pubescens Turcz. The optimal chromatographic conditions were achieved on a Zorbax C18 column using gradient elution with 0.5% aqueous acetic acid and acetonitrile as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was developed as follows: 0–10 min, 276 nm; 10–45 min, 332 nm. The validation of the method including linearity, precision, stability, accuracy, repeatability and recovery was tested. The chemometric analysis including hierarchical cluster analysis and principal component analysis was employed to investigate the similarity and difference of samples from different geographical origin. The results revealed that S. pubescens samples were divided into four clusters based on the phenylethanoid glycosides contents. Antioxidant activity of extract was measured using three different methods including α,α-diphenyl-β-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical scavenging assays, and ferric reducing antioxidant power assay. Furthermore, different phenylethanoid glycosides exhibited different contribution to antioxidant capacities. This study provides a foundation for the quality evaluation and offers scientific data for the utilization of S. pubescens resources.

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