查找使用阿拉丁产品的科研文献

Chitosan-cucurbit[5]uril supramolecular gel-induced room-temperature phosphorescence: A doxorubicin-responsive RTP probe based on PRET mechanism

Jin-Xian Luo,Qing Fan,Qing Tang,Zhu Tao,Ying Huang

A supramolecular gel was constructed based on chitosan (CS) and cucurbit[5]uril (Q[5]) through self-assembly via hydrogen bonds and ion-dipole interactions. This gel possesses a dense three-dimensional network structure that provides a rigid microenvironment for encapsulating 6-bromo-2-methylquinoline (6-Br-2-MQ), successfully inducing room-temperature phosphorescence (RTP) emission at 511 nm with a phosphorescence lifetime of 1.537 ms and a quantum yield of 6.72%. Mechanistic studies indicate that the RTP emission originates from the synergistic effects of the rigid gel matrix, host-guest interactions at the Q[5], and the bromine-induced heavy-atom effect. The RTP system exhibits reversible temperature-responsive “on-off” behavior. Furthermore, based on a resonance energy transfer mechanism, the system serves as a phosphorescence probe for the detection of doxorubicin (DOX) in complex biological matrices with a detection limit of 0.65 μM, utilizing a PRET-based dynamic quenching mechanism. The probe shows good recovery (96.08–100.75%) and high anti-interference capability in artificial urine samples. This study provides a new strategy for developing RTP materials using naturally derived and biocompatible components, and demonstrates their potential as a simple, cost-effective platform for point-of-care biosensing applications in complex biological fluids.

Leonurus cardiaca L. polysaccharide-stabilized Pd-Au nanozymes for NIR-responsive doxorubicin delivery and combined chemo-chemodynamic-photothermal therapy

Ruyu Li,Tingting Zhang,Lijie Zhou,Jingjing Feng,Longgang Wang,Haiyan Xiao

Pd 3 Au-LCLP NPs-DOX with anti-protein adsorption properties was prepared. • Pd 3 Au-LCLP NPs-DOX exhibit good enzyme-like activity and photothermal performance. • More DOX was released under the irradiation of 808 nm laser. • Pd 3 Au-LCLP NPs-DOX shows significant anti-tumor effects by multi-therapy. Traditional cancer therapies are limited by severe side effects and therapeutic challenges, while noble metal nanozymes offer promising enzyme-like therapeutic potential but remain constrained by poor aqueous stability. In this work, palladium-gold bimetallic nanoparticles (Pd 3 Au-LCLP NPs) with peroxidase-like activity were constructed using Leonurus cardiaca L. polysaccharide as both a reducing agent and stabilizing template, and further loaded with doxorubicin (DOX) to form Pd 3 Au-LCLP NPs-DOX. The resulting nanoplatform exhibited excellent water dispersibility, structural stability, anti-protein adsorption capability, and enhanced photothermal conversion performance. Under 808 nm laser irradiation, Pd 3 Au-LCLP NPs-DOX achieved a cumulative DOX release of 81% at pH 5.5. In vitro , it showed significant cytotoxicity against A549 and HeLa cells. In vivo , the tumor inhibition rate reached 82.6%. Overall, this combined therapeutic strategy integrating chemodynamic therapy (CDT), photothermal therapy (PTT), and chemotherapy (CT) demonstrated strong synergistic anti-tumor efficacy. Download: Download high-res image (393KB) Download: Download full-size image

Folic Acid-Targeted PLGA Nanoparticles Co-delivering Doxorubicin and MnTPP Enable Ultrasound-Triggered Synergy and Apoptosis in Nasopharyngeal Carcinoma

Min Zhang,Chunmiao Chen,Dandan Sun,Dayan Yang,Lingling Qin,Yanhua Cui,Xiangxiang Jing

Although the co-delivery of chemotherapeutic agents and sonosensitizer has been studied for years, developing an efficiency co-delivery nanocarrier that combine chemotherapy and sonodynamic therapy (SDT) with precise targeting remains a challenge. In this study, we prepared a folic acid (FA)-functionalized poly (lactic-coglycolic acid) (PLGA) nanoparticle (FPMD NP) that co-loaded with doxorubicin (Dox) and the sonosensitizer manganese (III) meso-tetrakis(4-carboxyphenyl) porphyrin (MnTPP) via a double-emulsion solvent evaporation method. Upon ultrasound irradiation, oxidative stress and apoptotic signaling were activated, which revealed by transcriptomic analysis. In vivo, the combination of FPMD NPs with ultrasound achieved tumor targeting, and tumor growth suppression in BALB/c nude mice bearing HNE-1 xenografts. The body weight of BALB/c nude mice maintained stable and no histopathological abnormalities in major organs were detected. Overall, the FPMD NPs successfully integrate MnTPP-mediated SDT with Dox chemotherapy, and demonstrate strong antitumor efficacy and favorable biosafety, highlighting its potential for synergistic SDT and chemotherapy in nasopharyngeal carcinoma treatment.

Dual-functional injectable adhesive hydrogel delivering ginger-derived doxorubicin vesicles for osteosarcoma recurrence suppression and post-resection wound healing

Qiang Zhang,Yu Zhang,Hui Chen,Lei-Na Sun,Bin Zhang,Dong-Sheng Yue,Chang-Li Wang,Zhen-Fa Zhang

Introduction: Elderly osteosarcoma patients often face significant postoperative challenges, including high recurrence rates and delayed wound healing. These issues are primarily due to inadequate hemostasis, reactive oxygen species (ROS)-mediated microenvironmental inhibition, and compromised bone regeneration. This study aims to address these challenges by introducing a multifunctional adhesive hydrogel designed for synergistic therapy.Methods: The hydrogel consists of carboxymethyl chitosan methacryloyl (CMCSMA) and tannic acid (TA), which form a dynamic, crosslinked polymer network capable of rapid tissue adhesion and adaptability to moist wound environments. The hydrogel incorporates ginger vesicles (GVs) loaded with doxorubicin (DOX), offering a dual therapeutic approach. The system facilitates hemostasis through physical barrier formation and activation of coagulation factors, while GVs and DOX provide controlled release for ROS scavenging, reduction of inflammation, and targeted tumor cell elimination.Results: In vitro experiments demonstrated the hydrogel’s ability to efficiently remove ROS and promote osteogenic differentiation. In a rat osteosarcoma resection model, the hydrogel significantly shortened hemostasis time compared to conventional sponges, reduced tumor recurrence, and accelerated wound healing.Discussion: This study presents a multifunctional hydrogel that combines hemostasis, antioxidation, tissue repair, and recurrence prevention. The findings suggest that this integrated therapeutic approach holds substantial potential for clinical application in elderly osteosarcoma treatment, addressing critical postoperative challenges and improving patient outcomes.

Selective adsorption and determination of doxorubicin and epirubicin in plasma using magnetic molecularly imprinted polymers combined with HPLC-UV

Shukai Sun,Yue Zhai,Qingwu Tian,Peng Zhao,Hong Cheng,Deng Pan,Junna Sui,Yusun Zhou,Chao Xuan,Tingting Zhou

Magnetic molecularly imprinted polymers (MMIPs) were synthesized using magnetic Fe3O4@SiO2 as a carrier for the selective recognition and adsorption of doxorubicin (DOX) and epirubicin (EPI) in plasma. Then, the method was combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) to realize rapid and accurate detection of DOX and EPI concentrations in plasma. The structures of the prepared MMIPs were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy, and magnetic saturation. As observed, the prepared MMIPs had a uniform appearance and excellent magnetic properties. Then, the adsorption performance of MMIPs was verified through static, dynamic, and selective adsorption tests. MMIPs were found to have high adsorption capacity (8.36 and 8.19 mg g−1), high selectivity (imprinting factors of 34.83 and 8.03), and rapid adsorption for DOX and EPI, respectively. Next, HPLC-UV was used for quantitative analysis. The prepared method had a good linear relationship (0.0001–0.5 mg mL−1 for DOX and 0.0002–0.5 mg mL−1 for EPI) and low detection limits (0.04613 ng mL−1 for DOX and 0.08969 ng mL−1 for EPI). The combination of magnetic solid-phase extraction and HPLC-UV was a highly specific and sensitive method for determining DOX and EPI concentrations in plasma.

Laser-programmable glycosaminoglycan-based nanocarriers co-deliver hypocrellin B and doxorubicin for spatiotemporal chemo-photothermal therapy of hepatocellular carcinoma

Muhammad Sohail,Kai Li,Ayesha Younas,Sajid Iqbal,Xiaoxiao Chen,Baozhu Yang,Gaofeng Shu,Minjiang Chen,Hong Yuan,Jiansong Ji

CS-DHB NPs enabled pH-/laser-triggered DOX and HB codelivery for spatiotemporal HCC chemo-photothermal therapy. • Under 660 nm laser (1.0 W cm⁻²), NPs exhibited rapid hyperthermia, high ¹O₂ yield, and superior photostability. • In Hepa1-6 tumor models, NPs plus laser ensured 100% survival, marked tumor regression, and no notable toxicity. Hepatocellular carcinoma (HCC) remains lethal due to systemic chemotherapy is halted by cardiotoxicity, multidrug resistance, and poor tumor penetration. To address these limitations, we developed a novel modified chondroitin sulfate/cystamine nanoparticle, termed CS-DHB NPs, that co-delivers doxorubicin (DOX) and the photosensitizer hypocrellin B (HB) and is encapsulated within a chondroitin sulfate/cystamine nanocarrier. These engineered nanoparticles (∼154 nm) demonstrated high drug-loading efficiencies (DOX: 80%; HB: 60%), exceptional serum stability (>72 h), and dual-drug release in laser- and pH-dependent environments. Upon 660 nm laser irradiation (1.0 W cm⁻²), the CS-DHB NPs produced substantial photothermal, photodynamic effects (¹O₂ quantum yield: 0.56), and excellent photostability. The prepared CS-DHB NPs were biocompatible and showed efficient in vitro cytotoxic effects via CCK-8 assays and deep penetration and inhibition of multicellular tumor spheroids (MTS). Ex vivo cellular studies revealed that the targeted delivery mechanism, clathrin-dependent endocytosis, with laser-triggered uptake, enhanced intracellular DOX accumulation twofold. In Hepa1–6 cells, CS-DHB NPs treatment induced pronounced apoptosis (∼51%). In vivo, five intravenous administrations combined with localized laser irradiation resulted in significant tumor regression, 100% survival, minimal systemic toxicity, and no histopathological damage in a Hepa1–6-bearing C57BL/6 mice model. By integrating chemotherapy, phototherapy, and targeted release within a tumor-targeted nanosystem, CS-DHB NPs offer a promising strategy to overcome chemoresistance while preserving healthy tissues, advancing an effective, and clinically translatable approach for synergistic HCC therapy.

Tumor Microenvironment Responsive Nanomicelle with Folic Acid Modification Co-Delivery of Doxorubicin/Shikonin for Triple Negative Breast Cancer Treatment

Wu Zhong,Zhehao Shen,Menglan Wang,Hongyi Wang,Yuting Sun,Xiaojun Tao,Defu Hou

Triple negative breast cancer (TNBC), which has poor prognosis, easily develops drug resistance and metastasizes. In general, those TNBC characteristics are related to a high activation of the epithelial-mesenchymal transition (EMT) pathway, which is inhibited by shikonin (SKN). Therefore, the synergistic therapy of SKN and doxorubicin (DOX) will increase anti-tumor efficacy and reduce metastasis. In this study, we prepared the folic acid-linked PEG nanomicelle (NM) grafted with the DOX (denoted as FPD) to load the SKN. We prepared the[email protected]NM according to the effective ratio of dual drugs, where the drug loadings of DOX and SKN were 8.86 ± 0.21% and 9.43 ± 0.13%, with 121.8 ± 1.1 nm of its hydrodynamic dimension and 6.33 ± 0.16 mV of zeta potential, respectively. The nanomaterials significantly slowed down the release of DOX and SKN over 48 h, leading to the release of pH-responsive drugs. Meanwhile, the prepared NM inhibited the activity of MBA-MD-231 cells in vitro. Further in vitro study revealed that the[email protected]NM increased the DOX uptake and significantly reduced the metastasis of MBA-MD-231 cells. Overall, these active-targeting NMs improved the tumor-targeting of small molecular drugs and effectively treated TNBC.Keywords:nanomicelles;doxorubicin;shikonin;tumor microenvironment-sensitive;TNBC;targeted therapy

GE11 peptide modified and reduction-responsive hyaluronic acid-based nanoparticles induced higher efficacy of doxorubicin for breast carcinoma therapy

Danrong Hu,Omar Mezghrani,Lei Zhang,Yi Chen,Xue Ke,Tianyuan Ci

Novel breast carcinoma dual-targeted redox-responsive nanoparticles (NPs) based on cholesteryl-hyaluronic acid conjugates were designed for intracellular delivery of the antitumor drug doxorubicin (DOX). A series of reduction-responsive hyaluronic acid derivatives grafted with hydrophobic cholesteryl moiety (HA-ss-Chol) and GE11 peptide conjugated HA-ss-Chol (GE11–HA-ss-Chol) were synthesized. The obtained conjugates showed attractive self-assembly characteristics and high drug loading capacity. GE11–HA-ss-Chol NPs were highly stable under conditions mimicking normal physiological conditions, while showing a fast degradation of the vehicle’s structure and accelerating the drug release dramatically in the presence of intracellular reductive environment. Furthermore, the cellular uptake assay confirmed GE11–HA-ss-Chol NPs were taken up by MDA-MB-231 cells through CD44- and epidermal growth factor receptor-mediated endocytosis. The internalization pathways of GE11–HA-ss-Chol NPs might involve clathrin-mediated endocytosis and macropinocytosis. The intracellular distribution of DOX in GE11–HA-ss-Chol NPs showed a faster release and more efficient nuclear delivery than the insensitive control. Enhanced in vitro cytotoxicity of GE11–HA-ss-Chol DOX-NPs further confirmed the superiority of their dual-targeting and redox-responsive capacity. Moreover, in vivo imaging investigation in MDA-MB-231 tumor-bearing mice confirmed that GE11–HA-ss-Chol NPs labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide, a near-infrared fluorescence dye, possessed a preferable tumor accumulation ability as compared to the single-targeting counterpart (HA-ss-Chol NPs). The antitumor efficacy showed an improved therapy efficacy and lower systemic side effect. These results suggest GE11–HA-ss-Chol NPs provide a good potential platform for antitumor drugs.

Delivery of Doxorubicin by Ferric Ion-Modified Mesoporous Polydopamine Nanoparticles and Anticancer Activity against HCT-116 Cells In Vitro

Mengwen Guo,Junhong Ling,Xinyi Xu,Xiaokun Ouyang

In clinical cancer research, photothermal therapy is one of the most effective ways to increase sensitivity to chemotherapy. Here, we present a simple and effective method for developing a nanotherapeutic agent for chemotherapy combined with photothermal therapy. The nanotherapeutic agent mesoporous polydopamine-Fe(III)-doxorubicin-hyaluronic acid (MPDA-Fe(III)-DOX-HA) was composed of mesoporous polydopamine modified by ferric ions and loaded with the anticancer drug doxorubicin (DOX), as well as an outer layer coating of hyaluronic acid. The pore size of the mesoporous polydopamine was larger than that of the common polydopamine nanoparticles, and the particle size of MPDA-Fe(III)-DOX-HA nanoparticles was 179 ± 19 nm. With the presence of ferric ions, the heat generation effect of the MPDA-Fe(III)-DOX-HA nanoparticles in the near-infrared light at 808 nm was enhanced. In addition, the experimental findings revealed that the active targeting of hyaluronic acid to tumor cells mitigated the toxicity of DOX on normal cells. Furthermore, under 808 nm illumination, the MPDA-Fe(III)-DOX-HA nanoparticles demonstrated potent cytotoxicity to HCT-116 cells, indicating a good anti-tumor effect in vitro. Therefore, the system developed in this work merits further investigation as a potential nanotherapeutic platform for photothermal treatment of cancer.Keywords:mesoporous polydopamine;ferric ions;doxorubicin (DOX);hyaluronic acid target modification

Cetuximab-Coated Thermo-Sensitive Liposomes Loaded with Magnetic Nanoparticles and Doxorubicin for Targeted EGFR-Expressing Breast Cancer Combined Therapy

Buyankhishig Dorjsuren,Birendra Chaurasiya,Zixuan Ye,Yanyan Liu,Wei Li,Chaoyang Wang,Di Shi,Colin E Evans,Thomas J Webster,Yan Shen

Background One major limitation of cancer chemotherapy is a failure to specifically target a tumor, potentially leading to side effects such as systemic cytotoxicity. In this case, we have generated a cancer cell-targeting nanoparticle-liposome drug delivery system that can be activated by near-infrared laser light to enable local photo-thermal therapy and the release of chemotherapeutic agents, which could achieve combined therapeutic efficiency.Methods To exploit the magnetic potential of iron oxide, we prepared and characterized citric acid-coated iron oxide magnetic nanoparticles (CMNPs) and encapsulated them into thermo-sensitive liposomes (TSLs). The chemotherapeutic drug, doxorubicin (DOX), was then loaded into the CMNP-TSLs, which were coated with an antibody against the epidermal growth factor receptor (EGFR), cetuximab (CET), to target EGFR-expressing breast cancer cells in vitro and in vivo studies in mouse model.Results The resulting CET-DOX-CMNP–TSLs were stable with an average diameter of approximately 120 nm. First, the uptake of TSLs into breast cancer cells increased by the addition of the CET coating. Next, the viability of breast cancer cells treated with CET-CMNP-TSLs and CET-DOX-CMNP-TSLs was reduced by the addition of photo-thermal therapy using near-infrared (NIR) laser irradiation. What is more, the viability of breast cancer cells treated with CMNP-TSLs plus NIR was reduced by the addition of DOX to the CMNP-TSLs. Finally, photo-thermal therapy studies on tumor-bearing mice subjected to NIR laser irradiation showed that treatment with CMNP-TSLs or CET-CMNP-TSLs led to an increase in tumor surface temperature to 44.7°C and 48.7°C, respectively, compared with saline-treated mice body temperature ie, 35.2°C. Further, the hemolysis study shows that these nanocarriers are safe for systemic delivery.Conclusion Our studies revealed that a combined therapy of photo-thermal therapy and targeted chemotherapy in thermo-sensitive nano-carriers represents a promising therapeutic strategy against breast cancer.

Enzyme/pH dual stimuli-responsive nanoplatform co-deliver disulfiram and doxorubicin for effective treatment of breast cancer lung metastasis

Peifu Xiao,Xiaoguang Tao,Hanxun Wang,Hongbing Liu,Yupeng Feng,Yueqi Zhu,Zhengzhen Jiang,Tian Yin,Yu Zhang,Haibing He,Jingxin Gou,Xing Tang

Chitosan coated pH/redox-responsive hyaluronic acid micelles for enhanced tumor targeted co-delivery of doxorubicin and siPD-L1

Panpan Song,Zhongxia Lu,Tianze Jiang,Wenwei Han,Xiangyan Chen,Xia Zhao

The complex tumor microenvironment (TME) makes it difficult for single chemotherapy to achieve satisfactory therapeutic effects. Here, chitosan-coated hyaluronic acid micelles (R/C/ [email protected] ) that co-delivers doxorubicin (DOX) and programmed death-ligand 1 small interfering RNA (siPD-L1) are developed to enhance anti-tumor effect by combination of immunotherapy and chemotherapy. The pH/reduction dual-responsive co-delivery micelles R/C/ [email protected] are spherical particles about 180 nm, and have good drug loading performance, stability, biocompatibility, and TME-responsive drug release properties. The CD44 receptor targeting HA significantly enhances the cellular uptake of DOX and siPD-L1, and siPD-L1 causes the immune infiltration of CD4 + /CD8 + T cells by silencing PD-L1 expression. In vivo studies show that R/C/ [email protected] exhibits significantly stronger anti-breast cancer effect than that of free DOX and micelles loaded only DOX. Therefore, the dual-stimulus responsive micelles provide a promising strategy for combining chemotherapy and siRNA-based immunotherapy to enhance efficacy.