Journal of Analysis and Testing
·
2019
·Vol. 3
·期 3
·277-285
·
IF 0
Li Xinxue,Huang Qingwei,Li Wei,Zhang Jinli,Fu Yan
Abstract N -Acety-L-cysteine (NAC), enriched with multiple electron-rich groups involving –SH, –C=O, and –COOH, exhibits strong affinities to transition metal ions. Herein, NAC was adopted as the stabilizing agent to synthesize a series of peroxidase-like Pt nanoclusters (NCs) with different physicochemical properties. Both the cluster size and the charge state of NAC–Pt NCs are highly dependent upon the molar ratio of [K 2 PtCl 4 ]/[NAC], contributing to different peroxidase mimicking activities. The most active Pt nanozyme (~ 1.7 nm), composed of 60% metallic Pt 0 and 40% positively charged Pt 2+ respectively, exhibits the K m of 0.132 mM for 3,3′,5,5′-tetramethylbenzidine and 35 mM for H 2 O 2 . Based on the heparin-activated enzymatic activity of NAC–Pt at pH 6.0, an ultrasensitive test was established for quantitative determination of heparin, giving the linear response of 0.5–20 μg/mL as well as the limit of detection of 2 × 10 −3 μg/mL. This proposed method is also applicable in biological fluid for practical applications. Such a colorimetric method possesses several advantages involving high sensitivity, short response, eco-friendly synthesis, low consumption of material and energy, free of expensive instrument, as well as simple operation. Graphic abstract
研究领域:
纳米技术
·
分析化学
·
生物化学
✓ 已验证
DOI
Nanoscale
·
2017
·Vol. 9
·期 47
·18699-18710
·
IF 7.367
Bing Tan,Huimin Zhao,Weihao Wu,Xuan Liu,Yaobin Zhang,Xie Quan
Two-dimensional (2D) metal–organic framework (MOF) nanosheets emerging as a new member of the 2D family have received significant research interest in recent years. Herein, we have successfully synthesized 2D copper-based MOF nanosheets with bimetallic anchorage using a facile two-step process at room temperature and ambient pressure, denoted as Cu(HBTC)-1/Fe3O4-AuNPs nanosheets. The as-synthesized 2D bimetallic MOF nanosheets displayed enhanced peroxidase-like activity with relatively high catalytic velocity and affinity for substrates compared with previously reported peroxidase mimics. Furthermore, their intrinsic peroxidase-like catalytic activity could be flexibly regulated by single-stranded DNA (ssDNA), exhibiting the enhancement of 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation or inhibition of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) oxidation due to the adsorption of ssDNA via π–π stacking. Accordingly, on the basis of their peroxidase-like activity, our prepared 2D bimetallic immobilized MOF nanosheets achieved ultra-sensitive detection of H2O2 with a linear range of 2.86 to 71.43 nM, and comparable detection performance for glucose with a linear range of 12.86 to 257.14 μM. By means of their controllable peroxidase-like activity, a versatile colorimetric sensing platform was developed which realized the detection of sulfadimethoxine (SDM) with a linear range of 3.57 to 357.14 μg L−1 and the limit of detection (LOD) of 1.70 μg L−1. With the multiplexed performance for detecting various targets, our as-synthesized bimetallic MOF nanosheets hold great promise for applications in environmental monitoring, as well as bioassays by virtue of their good biocompatibility.
研究领域:
纳米材料科学
·
生物医学工程
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2013
·
IF 5.008
Lina Zhong,Feifei Xing,Wei Shi,Liuming Yan,Liqing Xie,Shourong Zhu
An aspartic acid-functionalized water-soluble perylene bisimide, N,N′-di(2-succinic acid)-perylene-3,4,9,10-tetracarboxylic bisimide (PASP) was synthesized and characterized. It has absorbance maximum A0–0 and A0–1 at 527 and 498 nm (ε ≈ 1.7 × 104 L cm–1 mol–1) respectively in pH 7.20 HEPES buffer. Two quasi-reversible redox processes with E1/2 at −0.17 and −0.71 V (vs Ag/AgCl) respectively in pH 7–12.5 aqueous solutions. PASP can react with Na2S in pure aqueous solution to form monoanion radical and dianion species consecutively. PASP–• has EPR signal with g = 1.998 in aqueous solution, whereas PASP2- is EPR silent. The monoanion radical formation is a first-order reaction with k = 8.9 × 10–2 s–1. Dianion species formation is a zero-order reaction and the rate constant is 4.3 × 10–8 mol L–1 s–1. The presence of H2O2 greatly increases the radical formation rate constant. PASP as a two-electron transfer reagent is expected to be used in the water photolysis.
研究领域:
有机化学
·
物理化学
·
电化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Shaoyu Tang,Lei Xu,Xiaolong Yu,Shuona Chen,Huanyong Li,Ying Huang,Junfeng Niu
Degradation of capecitabine was conducted using electrochemical oxidation, UV/H 2 O 2 oxidation, and ozonation. Capecitabine was efficiently degraded by three advanced oxidation processes. The degradation rates of capecitabine achieved approximately 99.0% after 4 min of ozonation, 12 min of electrochemical oxidation, and 30 min of UV/H 2 O 2 oxidation. The mineralization rate obtained was merely 10.3% for ozonation at 60 min, compared to 91.7% for electrochemical oxidation and 55.3% for UV/H 2 O 2 oxidation. Capecitabine was degraded by oxidation, defluorination, hydrogenation, hydrolysis, ring and bond cleavage. Thus, multiple intermediates were generated during the degradation process, such as F - , formic acid, acetic acid, oxalic acid, and other intermediate metabolites with lager molecules. A total of eighteen metabolites, except for F - and carboxylic acids, were identified by QTOF-HRMS, where six of them could only be detected by the electrochemical oxidation process, two of them were by the UV/H 2 O 2 oxidation process, and one of them by the ozonation process. Electrochemical oxidation was the only process that could achieve over 90% removal of cytotoxicity and genotoxicity, and the metabolites were non-genotoxic after 60 min of reaction. The number of live cells decreased with the increasing reaction time in ozonation, demonstrating the higher toxicity of the intermediates in ozonation. Energy cost evaluation results suggested that the lowest energy consumption was achieved at 1.8 kWh/m 3 for 90% of capecitabine degradation with the O 3 dosage of 250 mg/h. Energy consumptions for 50% removal of cytotoxicity were 21.3 and 6.3 kWh/m 3 in electrochemical oxidation and UV/H 2 O 2 oxidation, respectively. The highest energy cost for 50% of genotoxicity removal was observed in electrochemical oxidation, while ozonation was identified as the most cost-effective process.
研究领域:
环境科学
·
化学工程
·
药学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2015
·
IF 3.368
Wenshu Yang,Jinhui Hao,Zhe Zhang,Bailin Zhang
Starting with metal–organic frameworks (MOFs), a facile process was demonstrated toward the synthesis of porous FeP nanocubes. Herein, Prussian blue nanocubes were used as iron-based MOFs precursors, and converted into FeP nanocubes through the thermal phosphidation reaction. Then, the FeP nanocubes were exploited as a peroxidase mimetic, which not only has the advantages of low cost, high stability, and easy preparation, but also follows Michaelis–Menten behaviors and shows strong affinity to substrates. On the basis of high catalytic activity of FeP nanocubes, a rapid, and convenient approach was developed for the colorimetric detection of H 2 O 2 from 2 μM to 130 μM and with a detection limit of 0.62 μM. The good catalytic activity and high stability make the porous FeP nanocubes a useful catalyst for a wide range of potential applications in catalysis and biotechnology.
研究领域:
材料科学
·
生物技术
·
催化化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 48
·5851-5858
·
IF 2.896
Yan Guan,Xiao-Lan Zhao,Qiu-Xia Li,Long Huang,Jian-Mei Yang,Tong Yang,Yun-Hui Yang,Rong Hu
In this paper, a novel colorimetric strategy based on iodide ion (I−) and Cu-MOF catalysis was developed for simple, low-cost, and naked-eye detection of Fe3+. Both I− and MOFs display catalytic activity toward peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Adsorption and embedding of I− in Cu-MOF generates Cu-MOF/I with a three-dimensional porous structure that exhibits higher specific surface area, providing more active sites to facilitate interaction with TMB, resulting in enhanced catalytic efficiency. Reports have shown that Fe3+ can oxidize TMB in the absence of H2O2. We found that as Fe3+ concentration increases, the color of the system gradually deepens and the UV absorption peak gradually increases, thus providing a colorimetric sensor for quantitative Fe3+ detection. The detection limit (LOD) obtained in the presence of I− is 200 nM; however, in the absence of I−, the LOD is approx. 10 μM. Thus, the sensing system is ideal for signal amplified analysis of Fe3+. In the presence of various interfering metal ions, the developed sensing system displays excellent selectivity. Additionally, the practical application to Fe3+ detection in real samples is explored.
研究领域:
分析化学
·
材料科学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Wenya Hou,Wuyong Zhao,Sijing Yi,Wenmei Gao,Jiangang Zhang,Jinzhong Zhao,Xiaoxia Liu
Papain , as a classical cysteine protease , has been widely used in the food, pharmaceutical, chemical, and cosmetic fields. However, there are few information about the peroxidase-like activity of papain catalyzed substrate to produce fluorescence. In this study, we found that papain can catalyze H 2 O 2 to convert o-phenylenediamine (OPD), and generate fluorescence emission at 550 nm under 430 nm excitation. Based on this foundation, we report a papain/OPD/H 2 O 2 system for fluorescence detection of uric acid. The method exhibits a wide linear range of 10–1000 μM with a limit-of-detection of 4.6 μM, and has been successfully used to detect uric acid in human serum. This study paves the way for the application of papain as catalyst for fluorescence detection of different target biomolecules , such as cholesterol, glucose, lactate, for which H 2 O 2 is a product of oxidoreductase enzymes .
研究领域:
生物化学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 41
·8514-8524
·
IF 7.571
Qi Xu,Jiarui Wu,Yiming Feng,Han Hu,Zhimin Mo,Zushun Xu,Hewei Li,Shengli Yang
Sonodynamic therapy (SDT) is a non-invasive treatment, preferred by many researchers. Ultrasound is applied to tumor lesions, enriched with a sound sensitizer. The activation of the sound sensitizer produces reactive oxygen species (ROS), which kill the tumor cells. In this study, raspberry-like AgBiS2@PVP nanoparticles (NPs) were prepared, which were modified with polyvinylpyrrolidone (PVP). Finally, AgBiS2@PVP NPs with 261 nm diameter and a suitable band gap were successfully synthesized. These NPs could play a role in SDT by generating ROS when induced by ultrasonic stimulation. Moreover, the AgBiS2@PVP NPs could also catalyze H2O2 in the tumor microenvironment to produce ˙OH, equipping the NPs with chemodynamic therapy (CDT) properties. In vivo and in vitro experiments suggested that, compared to the single treatments, the combination of SDT and CDT had a clearer inhibitory effect on tumor cells.
研究领域:
纳米技术
·
癌症治疗
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Qing Li,Run Lu,Huiyu Jiang,Yuyang Zhou
Establishing an energy/water-saving bleaching process is highly expected by textile plants to reduce industrial carbon emission, wastewater discharge and general cost. Therefore, an efficient UVA-assisted N-[4-(triethylammoniomethyl)-benzoyl]- caprolactam-chloride (TBCC) -activated hydrogen peroxide (H 2 O 2 ) bleaching strategy at ambient temperature for cotton/spandex (C/S) fabric was developed in this study. Results reveal that 7.4 g/L of TBCC-activated H 2 O 2 (30%) achieves a slightly higher whiteness index (WI) on C/S fabrics than those only treated with 60 g/L H 2 O 2 (30%). The treated C/S fabric displays a good dyeability using direct dyes due to the qualified wettability. The mild pH process protects C/S fabrics from damage with a low tensile loss (less than 10%) in warp and weft directions. The WI of C/S fabric mainly depends on the concentration of TBCC/H 2 O 2 couple and radiation time. Moreover, the degradation mechanism of cotton pigment in UVA/TBCC/H 2 O 2 bleaching system was implemented including the identification of effective reactive oxygen species (ROS), tracking of the concentration variation of hydroxyl radical (HO•) under UVA irradiation, and the exploration of degrading kinetics and pathway of morin as the model of pigment impurity. Indicated by the degradation rate constant, both TBCC and UVA plays an accelerating role in enhancing the degradation efficiency. In the radical scavenging experiment, HO• and superoxide radical (O 2 – •) are two major ROS participating in the degradation. And therein, HO• makes larger contribution than O 2 – •. HO• concentration has a high positive correlation with radiation duration and TBCC dosage based on the fluorescence labelling method. Confirmed by LC-MS/MS analyses, 2,4,6-trihydroxybenzoic acid (MW: 170.12) and 2,4,-dihydroxybenzoic acid (MW: 154.12) are two major segments degraded from the oxidation and ring-opening of C-ring in morin. Finally, considering energy, water and chemical conservations, the condition i.e. 7.4 g/L of H 2 O 2 (30%) and 20 g/L of TBCC under 30 W UVA radiation for 1 h, is recommended, which significantly increases the WI by 46.5%, and saves about 90% energy and 50% water consumption compared with the conventional waterbath bleaching at 95 °C for 45 min when similar WI enhancement was achieved.
研究领域:
纺织科学与工程
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Jianan Li,Xinyong Li,Qidong Zhao,Zao Jiang,Moses Tadé,Shaobin Wang,Shaomin Liu
A novel photoelectrochemical (PEC) biosensing platform coupling with enzyme-induced biocatalytic precipitation (BCP) for H 2 O 2 was successfully constructed. TiO 2 nanotube arrays (TNs) were coated with polydopamine (PDA) and horseradish peroxidase (HRP) as the PEC electrode through a facile method. PDA was incorporated into the sensor system not only as a sensitizing agent to alter the optical properties of TiO 2 nanotube arrays and enhance the visible light (λ > 400 nm) absorption, but also as a bonding resin to HRP via Michael addition or Schiff base reaction. The immobilized HRP on the PEC electrodes could induce BCP reaction as the signal amplifier by oxidizing the 4-chloro-1-naphthol (4-CN) into insoluble products in the presence of H 2 O 2 . The quantitative monitoring of H 2 O 2 could be realized thanks to the photocurrent decrease caused by the BCP generated insoluble products deposited on the PEC electrode surface. The fabricated TNs@HRP/PDA PEC electrode for biosensing of H 2 O 2 exhibited a good sensitivity as well as selectivity in a detection range from 1 nM to 50 μM, with a detection limit of 0.7 nM.
研究领域:
生物传感
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Zi-Long Song,Yanan Hou,Feifei Bai,Jianguo Fang
Oxidative stress is constantly involved in the etiopathogenesis of an ever-widening range of neurodegenerative diseases. As a consequence, effective repression of cellular oxidative stress to a redox homeostatic condition is a promising and feasible strategy to treat, or at least retard the progression of, such disorders. Nrf2, a primary orchestrator of cellular antioxidant response machine, is responsible for detoxifying and compensating for deleterious oxidative stress via transcriptional activation of a diverse array of antioxidant biomolecules. In the framework of our persistent interest in disclosing small molecules that interfere with cellular redox-regulating machinery, we report herein the synthesis, optimization, and biological assessment of 47 vinyl sulfone scaffold-bearing small molecules, most of which exhibit robust neuroprotective effect against H 2 O 2 -mediated lesions to PC12 cells. After initial screening, the most potent neuroprotective compounds 9b and 9c with marginal cytotoxicity were selected for the follow-up studies. Our results demonstrate that their neuroprotective effects are attributed to the up-regulation of a panel of antioxidant genes and corresponding gene products. Further mechanistic studies indicate that Nrf2 is indispensable for the cellular performances of 9b and 9c , arising from the fact that silence of Nrf2 gene drastically nullifies their protective action. Taken together, 9b and 9c discovered in this work merit further development as neuroprotective candidates for the treatment of oxidative stress-mediated pathological conditions.
研究领域:
神经科学
·
药物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2014
·Vol. 38
·期 10
·4931-4937
·
IF 3.159
Wei Xu,Yu Wang,Su Liu,Jinghua Yu,Hongzhi Wang,Jiadong Huang
In this work, we report a novel sandwich-type electrochemical aptasensor for sensitive and selective detection of antibiotic kanamycin. This aptasensor was constructed by assembling graphene–polyaniline (GR–PANI) and polyamidoamine dendrimer–Au nanoparticle (PAMAM–Au) nanocomposites on the surfaces of a glass carbon electrode, which as electrode materials exhibited excellent charge-transport property and loading capacity of biomolecules. Followed by the sandwich-type specific identification, kanamycin detection was realized by outputting a redox current from electro-reduction reaction of H2O2 catalyzed by HRP. It was demonstrated that the developed strategy had a wide linear response range from 5 × 10−6 to 4 × 10−2 μg mL−1 and a low detection limit of 4.6 × 10−6 μg mL−1. Additionally, this aptasensor had the advantages in its low cost, high sensitivity, and superb specificity. Thus, it might provide a useful tool for kanamycin determination and related food safety analysis and clinical diagnosis.
研究领域:
分析化学
·
食品安全
·
临床诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）