LUMINESCENCE
·
2019
·Vol. 34
·期 7
·680-688
·
IF 1.691
Yang Liu,Piaopiao Chen,Shan Zheng,Yichen Xing,Chaobiao Huang
In this study, a high fluorescence sensitivity and selectivity, molecularly imprinted nanofluorescent polymer sensor (MIP@SiO 2 @QDs) was prepared using a reverse microemulsion method. 2,4,6-Trichlorophenol (2,4,6-TCP) was detected using fluorescence quenching. Tetraethyl orthosilicate (TEOS), quantum dots (QDs) and 3-aminopropyltriethoxysilane (APTS) were used as cross-linker, signal sources and functional monomer respectively. The sensor (MIP@SiO 2 @QDs) and the non-imprinted polymer sensor (NIP@SiO 2 @QDs) were characterized using infra-red (IR) analysis, X-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The selectivity of MIP@SiO 2 @QDs was examined by comparing 2,4,6-TCP with other similar functional substances including 2,4-dichlorophenol (2,4-DCP), 2,6-dichlorophenol (2,6-DCP) and 4-chlorophenol (4-CP). Results showed that MIP@SiO 2 @QDs had better selectivity for 2,4,6-TCP than the other compounds. Fluorescence quenching efficiency displayed a good linear response at the 2,4,6-TCP concentration range 5–1000 μmol/L. The limit of detection (LOD) was 0.9 μmol/L (3σ, n = 9). This method was equally applicable for testing actual samples with a recovery rate of 98.0–105.8%. The sensor had advantages of simple pretreatment, good sensitivity and selectivity, and wide linear range and could be applied for the rapid detection of 2,4,6-TCP in actual samples.
研究领域:
分析化学
·
环境科学
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DOI
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JOURNAL OF POWER SOURCES
·
2020
·
IF 8.247
Yimin Yan,Yanping Hou,Zebin Yu,Lingli Tu,Shanming Qin,Guiyun Yuan,Danquan Lan,Shuo Chen,Jiangli Sun,Shuangfei Wang
The sluggish kinetics of cathodic oxygen reduction reaction (ORR) is one of the critical factors hindering the development of microbial fuel cells (MFCs). Herein, the metal organic framework (MOF)-derived Fe x Co 3-x S 4 /Fe y Co 9-y S 8 (FeCoS(MOF)) is in-situ grown on nickel foam as ORR catalyst with high doping of N and S. Results show that based on the role of FeCoMOF, the FeCoS(MOF) microspheres are made up of nanosheets piling-up without phase aggregation and structural collapse. Meanwhile, more defects are generated, resulting in low internal resistance, high quality activity and high turnover frequency . In the MFC with the FeCoS(MOF) cathode, the maximum power density is 2.55 times of that of Pt/C cathode. The excellent ORR activity is due to the unique morphology and rich oxygen defects, which shortens electronic transmission distance, facilitates the exposure of more active sites, and improves oxygen adsorption and conductivity. Moreover, the durability of FeCoS(MOF) cathode outperforms Pt/C cathode, with stable output voltage maintains for 2 months, while the Pt/C cathode decreases to 67% of the initial voltage. This work provides inspiration for developing efficient MOF-derived ORR catalyst.
研究领域:
材料科学
·
环境科学
·
能源科学
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TALANTA
·
2026
·
IF 6.7
Jie Zan,Wenjie Guo,Wencheng Tan,Jieying Lan,Tianxu Li,Yongyu Huang,Rui Feng,Aoxue Gao,Chen Wang,Lei Gao,Zhiming Wu,Chaozhan Chen
Current diagnostic methods for Helicobacter pylori ( H. pylori ) infection suffer from critical limitations. Endoscopy is invasive, while stool antigen tests and urea breath tests require specialized laboratories and complex sample processing. To address these unmet needs, we present a novel saliva-based point-of-care platform for rapid and quantitative H. pylori detection. The platform consists of two innovative biosensors: a time-resolved fluorescent microsphere-lateral flow immunoassay strip (TRFM-LFIA) and an electrochemical immunosensor, specifically designed for UreB antigen detection in unprocessed saliva. A high-affinity monoclonal antibody pair (2-C2-F5/8-B5-E6) targeting non-overlapping UreB epitopes was developed through integrated recombinant antigen expression, hybridoma technology, and molecular docking. This antibody pair was applied to both biosensors using a double-antibody sandwich format, achieving high sensitivity (LOD: 91 pg/mL for TRFM-LFIA; 38.3 pg/mL for electrochemical immunosensor) and broad dynamic range (0.1–1000 ng/mL) without sample pre-treatment. Both biosensors demonstrated good specificity against common interferents, with reproducibility below 8% coefficient of variation, and 100% concordance with gold-standard diagnostics in 21 saliva samples (11 H. pylori -positive, 10 healthy controls). Crucially, this work enables direct saliva analysis, eliminating the need for invasive procedures and complex infrastructure, thereby offering a scalable and cost-effective solution for resource-limited settings.
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PubMed
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2022
·
IF 4.969
Linlin Cui,Hanping Xu,Yingrui An,Mincai Xu,Zijie Lei,Xiaojuan Jin
This work reports a three-dimensional N, S co-doped lignin-based carbon microsphere/graphene composite hydrogel (GH-NSCMS) as an electrode material for supercapacitors and a signal enhancement material for tetracycline molecularly imprinted electrochemical sensors (MIECS). As a supercapacitor electrode, GH-NSCMS electrode has a specific capacitance of 434.6 F g −1 at 0.5 A g −1 , which can still maintain 94.15% after 5,000 cycles. In addition, a highly sensitive MIP electrochemical sensor for tetracycline detection is prepared based on GH-NSCMS composite material due to the synergistic effects of the high recognition accuracy of the imprinting method, and excellent conductivity of the composite hydrogel material. The sensor has a wide linear range (0.1–50 μM) and a significant detection limit (5 × 10 −8 mol L −1 ). Therefore, the 3D GH-NSCMS composites described herein have potential application prospects in supercapacitor electrode materials and tetracycline molecular imprinting detection.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Mianxing Luo,Meng Zhang,Changbiao Chi,Guo Chen
It is of great significance to study the effect of multi-enzyme aggregation behavior at the interface on the formation of multi-enzyme complexes and their co-catalytic characteristics, which is helpful for us to design and construct immobilized multi-enzyme complex systems for in vitro synthetic biology. Here, a magnetic microsphere with chelated Ni 2+ , was prepared to explore the self-assembly characteristics of PduQ-SpyTag (P-T) and Nox-SpyCatcher (N C) on its surface, based on the mixed interaction mode consisting the affinity of His-tag/Ni 2+ and covalent binding of SpyTag/SpyCatcher. After studying the effect of saturated or unsaturated adsorption of P-T on the covalent binding between P-T and N C at the interface, a possible multienzyme interaction mechanism for the affinity-assisted covalent self-assembly on the Ni 2+ chelating surface was proposed. The time evolution of NADH showed that the immobilized P-T/N-C complex formed by this method and the free P-T/N-C complex exhibited similar synergistic catalytic properties, and presented higher catalytic efficiency than the simple mixing of P-T and N C. The optimal catalytic conditions, stability and reusability of the immobilized multi-enzyme complexes prepared in this study were also discussed by comparing them with free enzymes. In this study, we demonstrate a simple and effective strategy for self-assembling SpyTag/SpyCatcher fusion proteins on the surface of magnetic beads, which is inspiring for the construction of more cascade enzyme systems at the interface. It provides a new method for facilitating the rapid construction of immobilized multi-enzyme complexes in vitro from the crude cell lysis .
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PubMed
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yilin Wang,Xi Tang,Zixia Zhao,Zhifang Liu,Caiyu Ge,Kunxiang Deng,Yixi Xie,Pengcheng Zhao,Junjie Fei
Tert -Butylhydroquinone (TBHQ) is a phenolic antioxidant commonly found in edible oils. However, excessive amounts of TBHQ can adversely affect food quality due to its toxic and mutagenic effects, which may cause human injury and present carcinogenic risks. Thus, there is an urgent need to develop a sensitive and accurate analytical method for the rapid detection of TBHQ. In this work, hierarchical flower-structured spherical SnIn 4 S 8 and pyramidal Bi 2 O 3 were combined to obtain the Z-scheme SnIn 4 S 8 /Bi 2 O 3 heterojunction. Under irradiation, the electron transport channel of this heterojunction facilitated the combination of electrons in the conduction band of Bi 2 O 3 with holes in the valence band of SnIn 4 S 8 , which decreased the recombination rate of photogenerated electrons and improved the photoelectric response. After the addition of TBHQ, the adsorption effect on the electrode surface and the oxidation reaction with holes in the valence band of Bi 2 O 3 further promoted the charge transfer and separation of photogenerated electrons and holes, which led to a rapid increase in the photocurrent to realize the detection of TBHQ in environmental and food samples. The increase in photocurrent has been linearly correlated with the logarithm of the concentration of TBHQ in the range of 1.00–500 μM with a limit of detection of 0.526 μM. Furthermore, the sensor showed excellent selectivity in the presence of a wide range of analogs. This study provides a novel scheme for the precise detection of TBHQ and the construction of high-performance photoelectrochemical Z-scheme heterojunctions.
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TALANTA
·
2013
·
IF 3.498
Weina Wang,Ruiyang Ma,Qiuhua Wu,Chun Wang,Zhi Wang
In this study, a magnetic microsphere-confined graphene (Fe 3 O 4 @SiO 2 -G) was prepared as a novel adsorbent for the preconcentration of some phthalate esters in environmental water and soybean milk samples prior to high performance liquid chromatography analysis. The properties and morphology of the Fe 3 O 4 @SiO 2 -G were characterized by transmission electron microscopy and X-ray diffraction. This novel graphene-based magnetic nanocomposite showed great adsorptive ability toward the analytes. The method, which takes the advantages of both the high adsorption capacity of G and easy phase separation of the magnetic adsorbent from the sample solution, could avoid the time-consuming experimental procedures related to the traditional solid phase extraction . Various experimental parameters that could affect the extraction efficiencies, such as the amount of Fe 3 O 4 @SiO 2 -G, the extraction time, the pH of sample solution and the desorption conditions, were investigated. Under the optimum conditions, the limits of detection ( S / N =3) of the method for the compounds were between 0.07 and 0.10 ng mL −1 in water samples, and between 0.15 and 0.30 ng mL −1 in soybean milk samples. The relative standard deviations (RSDs) varied from 2.7% to 6.1% ( n =5). The recoveries of the method were in the range between 87.2% and 109.0% for environmental water and soybean milk samples. The method is suitable to determine the five phthalate esters (diallyl phthalate, di- n -propyl-phthalate, benzyl butyl phthalate, dicyclohexyl-phthalate and diethyl-hexyl-phthalate) in environmental water and soybean milk samples.
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POLYMER DEGRADATION AND STABILITY
·
2026
·
IF 8.1
Ziyang Zhang,Wen Wang,Xiaoyang Guo,Jing He,Zaihang Zheng,Xin Liu,Ye-Tang Pan
High-performance silicone rubber (SR) was widely considered for engineering applications. However, its practical potential was constrained by inherent flammability. The prevalent reliance on halogen- and phosphorus-based flame retardants would be associated with long-term health and ecological concerns, while also raising the sustainability issues related to the progressive depletion of phosphate resources. In addition, the high loadings of inorganic fillers were often difficult to simultaneously maintain the strength and ductility of SR. To address the intrinsic limitation of SR with poor char-forming ability and the absence of effective flame-retardant pathway during combustion, a “single-component and multifunctional integration” strategy was adopted in place of conventional “multi-component stacking” method. A halogen-free, phosphorus-free, low-toxicity, and low-loading single-component flame retardant (Si-FMs@PANI@LDH) was constructed to enable the synergistic optimization of flame retardancy, mechanical performance, and corrosion-resistant durability. Specifically, guided by a brick-and-mortar-inspired hierarchical architecture, nitrogen-rich furfural-based microspheres (FMs) together with cobalt-iron layered double hydroxide (CoFe-LDH) nanosheets were employed as the principal structural domains, while polyaniline (PANI) and epoxy-modified silicone resin (EPSR) served as organic interfacial components. Through this design, thermal shielding, gas-phase dilution, residue reinforcement, and migration suppression were integrated into a single flame-retardant architecture. At a loading of only 13 phr, the resulting composites were upgraded to Underwriters Laboratories standards-94 (UL-94) V-0 rating, and the limiting oxygen index (LOI) was increased to 30.5%. Fire-hazard parameters were substantially reduced with total heat release reduction of 21.3% and total smoke release reduction of 21.9%. The production of CO and CO 2 was simultaneously suppressed. Moreover, the effective protection was maintained under extreme thermal shock above 1000 °C, and the glow-wire flammability index (GWFI) was higher than 960 °C, indicating high heat tolerance and self-extinguishing capability relevant to electrical safety scenarios. While the flame retardancy was enhanced, the mechanical reliability was also retained that was evidenced by the good self-supporting capability of SR-5 and its elongation at break of 253%. Under strongly alkaline corrosion condition, SR composites still maintained UL-94 V-0 rating after 120 days immersion tests, demonstrating excellent long-term durability and flame-retardancy retention. Overall, the hierarchical interfacial construction of Si-FMs@PANI@LDH provided a feasible route for achieving a multidimensional performance improvement without halogen or phosphorus elements. This work also offered a practical reference for developing flame-retardant SR with reduced resource dependence and improved service stability.
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Regenerative Biomaterials
·
2025
·
IF 8.1
Wang Shujing,Liang Jiahao,Chen Yu,Liu Xianda,Tong Dongmei,Li Yupei,Zhao Weifeng,Su Baihai,Zhao Changsheng
Conventional hemodialysis and hemodiafiltration prove less effective at removing protein-bound uremic toxins (PBUTs) from the bloodstream of end-stage renal disease patients, primarily because PBUTs cannot pass through the small pores in the polymeric membranes. Hemoperfusion is an extracorporeal blood purification technique that employs an adsorption mechanism to remove multiple uremic toxins from such patients. Yet, the efficacy of hemoperfusion is constrained by some limitations of contemporary adsorbents, such as suboptimal capacity to adsorb PBUTs and poor hemocompatibility, presenting significant barriers for their clinical application. To address these challenges, we engineered a tailored hemoperfusion adsorbent by compounding sulfonated polysulfone (SPSf) and polyethyleneimine (PEI) into polyethersulfone (PES) microspheres to effectively capture and remove PBUTs through electrostatic interactions. Specially, we introduced sulfuric acid into the coagulation bath to increase the adsorption amount of the developed adsorbent (H-PES/SPSf@PEI microspheres), to neutralize strong positive charge of PEI, and to improve hemocompatibility. The tailored H-PES/SPSf@PEI microspheres neither damage blood cells nor activate the complement pathway when they contact with human blood. Moreover, H-PES/SPSf@PEI microspheres have a high adsorption amount towards major PBUTs, including hippuric acid (HA, 34.24 mg/g), 3-indoleacetic acid (IAA, 49.19 mg/g), p-cresol sulfate (PCS, 40.31 mg/g) and indoxyl sulfate (IS, 128.67 mg/g) by fitting adsorption isotherms. In a simulated hemoperfusion setting, the removal ratio of IS, IAA, PCS and HA by H-PES/SPSf@PEI microspheres reach nearly 75.33%, 41.73%, 44.36%, and 21.11%, respectively, with 47.89% of IS, 40.64% of IAA, 44.42% of PCS and 37.35% of HA being removed from BSA solution. In conclusion, H-PES/SPSf@PEI microspheres hold a potential to eliminate PBUTs from patients with end-stage renal disease.
研究领域:
生物医学工程
·
肾脏病学
·
材料科学
·
生物工程
·
血液透析
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高影响因子
Biomedical Materials
·
2021
·Vol. 17
·期 1
·015014
·
IF 3.715
Qiuju Miao,Nan Jiang,Qinmeng Yang,Ismail Mohamed Hussein,Zhen Luo,Lei Wang,Shenyu Yang
Various requirements for the repair of complex bone defects have motivated to development of scaffolds with adjustable degradation rates and biological functions. Tricalcium phosphate (TCP) and calcium sulfate are the most commonly used bone repair materials in the clinic, how to better combine TCP and calcium sulfate and play their greatest advantages in the repair of osteoporotic bone defect is the focus of our research. In this study, a series of scaffolds with multistage-controlled degradation properties composed of strontium-doped calcium sulfate (SrCSH) and strontium-doped tricalcium phosphate (Sr-TCP) microspheres scaffolds were prepared, and their osteogenic activity, in vivo degradation and bone regeneration ability in tibia of osteoporotic rats were evaluated. In vitro studies revealed that different components of SrCSH/Sr-TCP scaffolds significantly promoted the proliferation and differentiation of MC3T3-E1 cells, which showed a good osteogenic induction activity. In vivo degradation results showed that the degradation time of composite scaffolds could be controlled in a large range (6–12 months) by controlling the porosity and phase composition of Sr-TCP microspheres. The results of osteoporotic femoral defect repair showed that when the degradation rate of scaffold matched with the growth rate of new bone, the parameters such as bone mineral density, bone volume/total volume ratio, trabecular thickness, angiogenesis marker platelet endothelial cell adhesion molecule-1 and new bone formation marker osteocalcin expression were higher, which promoted the rapid repair of osteoporotic bone defects. On the contrary, the slow degradation rate of scaffolds hindered the growth of new bone to a certain extent. This study elucidates the importance of the degradation rate of scaffolds for the repair of osteoporotic bone defects, and the design considerations can be extended to other bone repair materials, which is expected to provide new ideas for the development of tissue engineering materials in the future.
研究领域:
生物材料学
·
骨组织工程
·
医学
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PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2026
·
IF 8.8
Yuxing Han,Yingqing Zhan,Jie Liu,Jia Luo,Jing Yang,Sama M. Al-Jubouri,Xiaoming Gao,Xinyue Duan,Wei Zhao,Ping Zhang,Yajie Lei
Hydrogel-based polymer separation membranes are widely applied in the purification of oily emulsified wastewater. However, the traditional hydrogel layer on the membrane surface encounters major obstacles of poor structural stability and difficulty in addressing complex fouling issues. Herein, the 3D raspberry-like CdS@MXene heterojunction microsphere was prepared via a combined strategy of template-assisted and hydrothermal methods, which was applied as a functional nanofiller to reinforce the polyvinyl alcohol-tannic acid (PVA-TA) hydrogel decorated poly (arylene ether nitrile) (PEN) membrane with photothermal-catalytic functionality. Benefiting from the multi-scale synergistic stabilization strategy of multiple bonding, gradient functionalization, and nano-interpenetration enhancement, the hydrogel layer demonstrated robust structural stability under harsh conditions and physical damage. Furthermore, the 3D hierarchical heterojunction microsphere provided a rapid transport pathway for photogenerated electrons and effectively suppressed electron-hole recombination, conferring on the membrane high degradation efficiency of organic dyes under simulated sunlight conditions within 60 min (degradation rate: up to 96.88%). Simultaneously, the membrane surface temperature reached 85.34 °C via rapid photothermal conversion within 30 s, significantly reducing the viscosity of highly viscous oil pollutants attached to the membrane surface and achieving efficient self-cleaning capability. Moreover, 3D MXene-based heterojunction microsphere overcame the compact physical stacking of 2D nanosheets, enabling the efficient separation of oil-in-water emulsions (flux: 215.15 L m −2 h −1 ; rejection rate: 99.56%). This work provides a new approach for preparing robust and multiple antifouling hydrogel-based polymer separation membrane with photothermal-catalytic synergy for treating complex oily emulsified wastewater.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Shucheng Liu,Ying Sun,Danzhao Guo,Ruiheng Lu,Yuying Mao,Hongxiang Ou
For porous molecular imprinted polymers (MIPs), introduction of special functional groups and integration porous structure support will endow the MIPs with tunable surface properties and specific affinity performance. Herein, by combination of RAFT molecular imprinting, Pickering emulsion, and boronate affinity methods, a new kind of porous imprinted microspheres (BA-HPMIPs) using the low pKa value of APBA (1-allylpyridinium-3-boronic acid, pKa = 7.4) as functional monomer was prepared for specific molecular recognition and separation of NRG. Meanwhile, the novel RAFT functioned surfactants with dual amide multiple H-bonds were designed as stabilized particles was used to promote sustainability, which further confer them multifunctionality and/or by polymerizing the Pickering emulsion itself. Equilibrium adsorption was reached rapidly within 360 min, and the maximum equilibrium binding capacities of BA-HPMIPs were found to be 118.32 µmol g −1 for NRG. Furthermore, they had a stronger selectivity towards NRG than other competitive target (i.e rutin, catechol, ARS, o -nitrophenol). Moreover, BA-HPMIPs have shown outstanding reusability of 8.63 % loss in enrichment performance after five times repeatedly. As a proof of this concept, a commercially avaliable NRG with purity could be effectively extracted and concentrated to 96.75 % purity. These results demonstrated that, a new kind of porous imprinted microsphere with abundant porous structure and boronic acid recognizing sites has great potential for separation and purification of industrial samples under neutral conditions.
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危险品化学品经营许可证（带存储）