CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
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2023
·
IF 10.2
Rafael M. P. Poeys-Carvalho,Alice G. M. Gonzalez
Vegetables are crucial for a healthy human diet due to their abundance of essential macronutrients and micronutrients. However, there have been increased reports of antimicrobial-resistant Enterobacteriaceae isolated from vegetables. Enterobacteriaceae is a large group of Gram-negative bacteria that can act as commensals, intestinal pathogens, or opportunistic extraintestinal pathogens. Extraintestinal infections caused by Enterobacteriaceae are a clinical concern due to antimicrobial resistance (AMR). β-lactams have high efficacy against Gram-negative bacteria and low toxicity for eukaryotic cells. These antimicrobials are widely used in the treatment of Enterobacteriaceae extraintestinal infections. This review aimed to conduct a literature survey of the last five years (2018–2023) on the occurrence of β-lactam-resistant Enterobacteriaceae in vegetables. Research was carried out in PubMed, Web of Science, Scopus, ScienceDirect, and LILACS (Latin American and Caribbean Health Sciences Literature) databases. After a careful evaluation, thirty-seven articles were selected. β-lactam-resistant Enterobacteriaceae, including extended-spectrum β-lactamases (ESBLs)-producing, AmpC β-lactamases, and carbapenemases, have been isolated from a wide variety of vegetables. Vegetables are vectors of β-lactam-resistant Enterobacteriaceae, contributing to the dissemination of resistance mechanisms previously observed only in the hospital environment.
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PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
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2014
·Vol. 211
·期 12
·2773-2777
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IF 1.525
Beibei Li,Zhipeng Qiu,Qijin Wan,Yi Liu,Nianjun Yang
β-cyclodextrin (β-CD) and graphene nano platelets (GNPs) have been applied to construct the β-CD/GNP coated electrode and further for the determination of triclosan. The surface properties of β-CD/GNP coated electrodes were characterized with scanning electron microscopy (SEM) and electrochemical techniques. On such an electrode, the oxidation peak current of triclosan is three-times higher than that on the GNP coated electrode and seven-times higher than that on a bare glassy carbon electrode. By applying differential pulse voltammetry, the monitoring of tricosan in the range of 2.0 µM to 100 µM was realized. The detection limit was 0.6 µM. The β-CD and GNPs based sensor shows high sensitivity, wide dynamic response range, and long stability towards the detection of triclosan. This simple and sensitive electrochemical sensor is thus promising for the environmental monitoring of triclosan in future.
研究领域:
环境监测
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电分析化学
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纳米材料
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传感器与生物传感器
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TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
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2014
·Vol. 13
·期 11
·1791-1796
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IF 0.495
H Nan,H Ma,R Zhang,R Zhan
Purpose: To improve the hydrophilic property of myricetin by complexing it with hydroxypropyl-β- cyclodextrin (HP-β-CD)Methods: The complexes of myricetin and HP-β-CD were prepared in both water and ethanol. The physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and x-ray diffractometry (XRD).Results: The content of myricetin in the complex prepared in ethanol (3.71 %) was higher than that prepared in water (1.43 %). Characterization studies indicate that myricetin in the complex was molecularly dispersed in HP-β-CD matrix.Conclusion: Ethanol is the better solvent to prepare the complex of myricetin and hydroxypropyl-β- cyclodextrin. Myricetin and HP-β-CD in the complex are combined by non-covalent bond, and thus did not form a new compound.
研究领域:
药剂学
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药物递送
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分子包合
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分析化学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Cuicui Ding,Mengdie Tian,Yue Wang,Kuan Cheng,Yifan Yi,Min Zhang
The effect of carbohydrates on collagen self-assembly behavior has been widely investigated because of their regulation on collagen fibrogenesis in vivo. In this paper, β-cyclodextrin (β-CD) was selected as an external disturbance to explore its intrinsic regulating mechanism on collagen self-assembly. The results of fibrogenesis kinetics indicated that β-CD had a bilateral regulation on collagen self-aggregation process, which was closely related to the content of β-CD: collagen protofibrils with low β-CD content were less aggregated compared to collagen protofibrils with high β-CD content. However, typical periodic stripes of ~67 nm on collagen fibrils were observed from transmission electron microscope (TEM), indicating that β-CD did not disturb the lateral arrangement of collagen molecules to form a 1/4 staggered structure. Correspondingly, the degree of aggregation of collagen self-assembled fibrils was closely correlated with the addition of β-CD content, as confirmed by field emission scanning electron microscopy (FESEM) and atomic force microscope (AFM). In addition, collagen/β-CD fibrillar hydrogel had good thermal stability and cytocompatibility. These results provide a better understanding of how to construct a structurally reliable collagen/β-CD fibrillar hydrogel as a biomedical material in a β-CD-regulated environment.
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JOURNAL OF BIOTECHNOLOGY
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2023
·
IF 4.1
Zekang Wang,Haiyan Chen,Yuyue Qin,Tianqing Lan
In this study, the Fenton oxidized lignin was prepared to investigate the effect of Fenton oxidation modification on the activity of lignin immobilized β-glucosidase (β-GL). The results demonstrated that Fenton oxidation could significantly improve the activity and stability of immobilized β-GL. This is because the Fenton oxidation increased the electrostatic, hydrogen bonding, and hydrophobic forces between lignin and β-GL, resulting in increased lignin adsorption onto β-GL. The Fenton oxidation also changed the chemical structure of lignin, altering the lignin-β-GL binding site and reducing the negative effect of lignin on the β-GL catalytic domain . This research will improve understanding of the effect of Fenton lignin oxidation on immobilized β-GL activity and expand the use of lignin in enzyme immobilization .
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LANGMUIR
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2022
·
IF 4.331
Chaonan Li,Ya Wang,Zihao Wang,Xiaogang Li
Although paraquat is a widely used herbicide, it is toxic to humans if ingested or absorbed through an open wound. To improve the safety of paraquat, a new formulation of paraquat based on photoresponsive polymers was exploited. Photoresponsive β-cyclodextrin polymer microspheres (AZO-CD) were synthesized via a host–guest interaction between β-cyclodextrin and azobenzene. AZO-CD were characterized by Fourier transform infrared spectrometry, circular dichroism, ultraviolet (UV) spectrophotometry, and thermogravimetric analysis, and their photoresponsiveness was also evaluated. AZO-CD were used to load paraquat, which yielded photoresponsive paraquat-loaded microspheres. For the paraquat-loaded microspheres, irradiation with UV light or sunlight induced the isomerization of azobenzene into the cis form. Then, the cis-azobenzene was liberated from the cavities of the β-cyclodextrin. The paraquat-loaded microspheres released paraquat continuously over time. Furthermore, under UV light, the herbicidal capacity of the paraquat-loaded microspheres against barnyard grass was comparable to that of free paraquat at the same dose. Our findings show that loading paraquat into AZO-CD provides a safe and environmentally friendly herbicide formulation.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 24
·16055
·
IF 6.208
Yan-Ting Wu,Li-Shan Zhong,Chen Huang,Yu-Ying Guo,Fu-Jun Jin,Yu-Ze Hu,Zi-Bo Zhao,Zhe Ren,Yi-Fei Wang
Macrophage infiltration is one of the main pathological features of ulcerative colitis (UC) and ferroptosis is a type of nonapoptotic cell death, connecting oxidative stress and inflammation. However, whether ferroptosis occurs in the colon macrophages of UC mice and whether targeting macrophage ferroptosis is an effective approach for UC treatment remain unclear. The present study revealed that macrophage lipid peroxidation was observed in the colon of UC mice. Subsequently, we screened several main components of essential oil from Artemisia argyi and found that β-caryophyllene (BCP) had a good inhibitory effect on macrophage lipid peroxidation. Additionally, ferroptotic macrophages were found to increase the mRNA expression of tumor necrosis factor alpha (Tnf-α) and prostaglandin-endoperoxide synthase 2 (Ptgs2), while BCP can reverse the effects of inflammation activated by ferroptosis. Further molecular mechanism studies revealed that BCP activated the type 2 cannabinoid receptor (CB2R) to inhibit macrophage ferroptosis and its induced inflammatory response both in vivo and in vitro. Taken together, BCP potentially ameliorated experimental colitis inflammation by inhibiting macrophage ferroptosis. These results revealed that macrophage ferroptosis is a potential therapeutic target for UC and identified a novel mechanism of BCP in ameliorating experimental colitis.Keywords:ulcerative colitis;ferroptosis;β-caryophyllene;type 2 cannabinoid receptor;inflammation;macrophage
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ANALYTICAL CHEMISTRY
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2023
·
IF 7.4
Xiaoqing Tan,Yongtao Tang,Tingting Yang,Guoliang Dai,Changqing Ye,Jianxin Meng,Fengyu Li
Photochromic sensors have the advantages of diverse isomers for multi-analysis, providing more sensing information and possessing more recognition units and more sensitivity to external stimulations, but they present enormous complexity with various stimulations as well. Deep learning (DL) algorithms contribute a huge advantage at analyzing nonlinear and multidimensional data, but they suffer from nontransparent inner networks, “black-boxes”. In this work, we employed the explainable DL approach to process and explicate photochromic sensing. Spirooxazine metallic complexes were adopted to prepare a multi-state analysis array for β-Lactams identification and quantitation. A dataset of 2520 unduplicated fluorescence intensity images was collected for convolutional neural network (CNN) operation. The method clearly discriminated six β-Lactams with 97.98% prediction accuracy and allowed rapid quantification with a concentration range from 1 to 100 mg/L. The photochromic sensing mechanism was verified via molecular simulation and class activation mapping, which explicated how the CNN model assesses the importance of photochromic sensor states and makes a discrimination decision. The explainable DL-assisted analysis method establishes an end-to-end strategy to ascertain and verify the complicated sensing mechanism for device optimization and even new scientific discovery.
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CHEMICAL PAPERS
·
2023
·Vol. 77
·期 6
·2967-2976
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IF 2.2
Ma Mingming,Liang Juanli
The voltammetric behavior of 2-aminoazotoluene (2-AMAT) is studied at pencil electrode (PEC) modified by electropolymerization of β-cyclodextrin (P-β-CD-PEC) in different supporting electrolyte at various pH values using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). At the scan rate of 0.1 V s −1 , 2-AMAT exhibits an irreversible oxidation peak at 0.67 V in phosphate buffer solution (PBS) with the pH value of 6.64. The electrochemical data suggest that the oxidation reaction of 2-AMAT at the surface of P−β−CD−PEC involves one electron and one proton. 2−AMAT has a significantly higher oxidation peak current on P−β−CD−PEC than pristine PEC, which is ascribed to the presence of cavities in polymer of β−CD on the surface of PEC. Under the optimized DPV conditions, the oxidation peak current is linear with 2-AMAT concentration in the range of 0.3 μM to 30.0 μM with a low limit of detection (LOD = 0.14 μM) and high sensitivity (1.45 μA /μM). The same 2-AMAT concentration was measured every two days in 20 days using the same P−β−CD−PEC. The results show that the relative standard deviation (RSD) of the peak current was 3.36%, indicating that the stability of P−β−CD−PEC is good. Five different bitches P−β−CD−PEC was used to determine the same 2-AMAT concentration and 4.29% RSD is obtained, which suggests that the reproducibility of this modified electrode is excellent. In addition to some metal ions, the structural substances such as 2,4-diaminotoluene and 4-chloroaniline do not interfere the determination of 2-AMAT, suggesting that P−β−CD−PEC has fine selectivity. Therefore, a new method for detecting 2-AMAT based on P−β−CD−PEC is developed. This method is successfully used to determine 2−AMAT in rose lotion cosmetics with satisfied results.
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EUROPEAN POLYMER JOURNAL
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2023
·
IF 6
Yang Liu,Chuang Lang,Yaping Ding,Siyu Sun,Guangwei Sun
Chitosan (CS)/β-sodium glycerophosphate (β-GP) gels have low temperature sensitivity, which limits their biomedical application. The protonation and deprotonation of the CS amino groups are regulated by pH. It is possible to accelerate thermosensitive gelation by increasing the pH of the CS solution to maximize the degree of CS amino deprotonation and enhance hydrophobic interactions. In this study, a CS solution with a higher pH (HpHCS; pH 6.19, close to pKa 6.2) was obtained by means of the consumption of hydrogen ions using excessive chitosan. Raman spectroscopy showed that strong hydrogen bonds might exist in HpHCS. Gelation was accelerated significantly at 37 °C using HpHCS due to the decreased sol–gel transition temperature. Support vector machine and t-distributed stochastic neighbor embedding analyses confirmed that the gel derived from HpHCS was significantly different from that of the other groups. In addition, the HpHCS gel exhibited a low degradation rate and low cytotoxicity. Furthermore, a mathematical equation was established to predict the gelation time of HpHCS/β-GP at 37 °C. A better concentration of HpHCS for in vivo injection was obtained due to the shorter gelation time and lower viscosity. We found that human mesenchymal stem cells survived and proliferated in vitro in the HpHCS/β-GP hydrogel. Therefore, this study provides an optimized HpHCS solution with enhanced deprotonation for accelerated thermosensitive gelation with β-GP.
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ENZYME AND MICROBIAL TECHNOLOGY
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2023
·
IF 3.4
Shuaifeng Liu,Meng Zhang,Dengwang Hong,Zemin Fang,Yazhong Xiao,Wei Fang,Xuecheng Zhang
β-Glucosidases with high catalytic activity and glucose tolerant properties possess promising applications in lignocellulose-based industries. To obtain enzymes possessing these properties, a semi-rational strategy was employed to engineer the glucose-stimulating β-glucosidase Bgl2A for high cellobiose hydrolysis activity. A total of 18 mutants were constructed. A22S, V224D, and A22S/V224D exhibited high specific activities of 272.06, 237.60, and 239.29 U/mg toward cellobiose, which were 2.5- to 2.8-fold of Bgl2A. A22S, V224D, and A22S/V224D exhibited increased k cat values, which were 2.7- to 3.1-fold of Bgl2A. A22S and V224D maintained glucose-stimulating property, whereas A22S/V224D lost it. Using 150 g/L cellobiose as the substrate, the amount of glucose produced by A22S was the highest, yielding 129.70 g/L glucose after 3 h reaction at 35 °C. The synergistic effects of the engineered enzymes with commercial cellulase on hydrolyzing cellulose were investigated. Supplemented with the commercial cellulase and A22S, the highest glucose amount of 23.30 g/L was yielded from cellulose with hydrolysis rate of 21.02 %. Given its high cellobiose hydrolysis activity and glucose-stimulating properties, A22S can be used as a component of enzyme cocktail to match mesophilic cellulases for efficient cellulose hydrolysis.
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Applied Nanoscience
·
2022
·Vol. 12
·期 9
·2683-2693
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IF 3.869
Chi Yu,Ye Zhenghe,Su Xianyan,Li Zhao,Ren Xuexiang
In this work, a compound was fabricated using β-cyclodextrin (β-CD) and propithiumazole (PTC). FTIR and TG were used to analyze the intermolecular force and thermal stability of the compound and results indicated. The intermolecular forces were van der Waals forces. According to previous research, the seed coat agent and suspension concentrate (SC) were prepared using this compound and auxiliary. The inhibitory effect on Fusarium graminearum was studied with a bioassay. The data suggested inhibition rate of microencapsulated PTC are about 7.8% and 5% higher than PTC at 72 h and 192 h, respectively. In addition, results of pot experiments suggested dry weight of crops were increased after using seed coat agent contained microencapsulated prothioconazole which indicates that the seed coat agent may be beneficial to protein synthesis. Compared to commercial PTC, field experiments were conducted to investigate the preventive effects of SC against fusarium head blight (FHB). The results of toxicity test and field experiment showed that this compound can improve the utilization efficiency about 5% of PTC and reduce pesticide injury to wheat seeds.
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