SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
·
IF 2.931
Dongwei Miao,Dongkui Liu,Yanbo Zeng,Guobao Zhou,Wei Xie,Yiwen Yang,Hailong Wang,Jian Zhang,Yunyun Zhai,Zulei Zhang,Lei Li
A novel fluorescent biosensor based on dabcyl [(E)-4-((4-(dimethylamino) phenyl) diazenyl)benzoic acid] -modified anti-Mb aptamer (D-AMA) and 6-FAM(6-carboxyfluorescein) -modified complementary short chain (F-CSC)for the specific and sensitive detection of Mb levels is presented in this study. In PBS buffer solution, D-AMA bound to F-CSC, and then dabcyl quenched the fluorescence of 6-FAM. After adding Mb into the system, D-AMA bound to Mb and separated from F-CSC. The fluorescence of 6-FAM was restored after it separated from dabcyl. The assay exhibited high specificity and sensitivity toward Mb, with a low limit of detection of 0.07 ng/mL (S/N = 3) and linear relationships of 0.1–5 ng/mL. It was further applied to detect Mb levels in spiked human blood sera samples.
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ANALYTICA CHIMICA ACTA
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2018
·
IF 5.123
Yifan Chen,Juan Qiao,Qianrong Liu,Mingming Zhang,Li Qi
Papain-stabilized gold nanoclusters ( [email protected] ) with a red fluorescence emission at 639 nm have been generated successfully in aqueous solution. The fluorescence of [email protected] could be quenched in the addition of Cu 2+ . Subsequently, a unique fluorescent probe based on [email protected] 2+ complex has been constructed for sensitive, selective and “turn-on” detection of D-penicillamine (D-pen). The sensing probe has exhibited a remarkable fluorescence enhancement in the presence of D-pen, which can be measured ranging from 30.0 μM to 2.0 mM (r 2 = 0.991) with the detection limit of 5.0 μM. Furthermore, the developed assay has been utilized to explore the metabolic process of D-pen in rats after intraperitoneal injection. As far as we concerned, the present study has reported the first analytical method for sensing serum D-pen in real time by using the fluorescent [email protected] 2+ complex. Our strategy paves a way for digging up the biological and clinical application of the fluorescent AuNCs.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2018
·Vol. 19
·期 10
·3184
·
IF 3.687
Zhiwen Qi,Chengzhang Wang,Jianxin Jiang,Caie Wu
A series of novel C15 urushiol derivatives were designed by introducing a pechmann structure and F-, Cl-, and Br-nitro substituents with different electronic properties into its alkyl side chain, as well as a triazolyl functional group in its aromatic oxide. Their chemical structures were determined based on the analysis of the NMR (nuclear magnetic resonance) spectroscopic and mass spectrometric data. The results showed that compound4exhibited a strong inhibition of the HepG2 cell proliferation (half maximal inhibitory concentration (IC50): 2.833 μM to human hepatocellular carcinoma (HepG2), and 80.905 μM to human normal hepatocytes (LO2)). Furthermore, it had an excellent synergistic effect with levopimaric acid. The nitrogen atom of the triazole ring formed a hydrogen-bonding interaction with Gly103, Gly154, and Tyr308, which made compound4bind to histone deacetylase (HDAC)2 more tightly. One triazole ring and His33 formed a π–π stacking effect; the other, whose branches were deep into the pocket, further enhanced the interaction with HDAC2. Meanwhile, compound4involved a hydrophobic interaction with the residues Phe210 and Leu276. The hydrophobic interaction and π–π stacking provided powerful van der Waals forces for the compounds.Keywords:urushiol;triazole;diels-alder;pechmann;anti-tumor;HDAC2
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RSC Advances
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2014
·Vol. 4
·期 66
·34944-34949
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IF 3.708
Jintao Lin,Shuangyue Li,Mingzhong Sun,Cuili Zhang,Wenbo Yang,Zehui Zhang,Xiaojie Li,Shao Li
The economical production of biodiesel from lignocellulose hydrolysates requires a robust D-xylose fermentation path. The present work found that Lipomyces starkeyi AS 2.1560 and Rhodosporidium toruloides AS 2.1389 could produce micro-lipid effectively from D-xylose solution by a two-stage culture mode. Results demonstrated that both L. starkeyi and R. toruloides could achieve high lipid production in xylose solution. High cell density fermentation was carried out by a feed-batch fermentation mode. After fermentation for 48 h in a bioreactor using unsterile xylose solution by L. starkeyi, the biomass, lipid and lipid content reached 97.4 g L−1, 63.9 g L−1, and 65.5%, respectively. Accordingly, the lipid productivity reached 1.3 g L−1 h−1 in the second stage. And the overall lipid productivity was 0.55 g L−1 h−1. Therefore, L. starkeyi and R. toruloides are highly effective D-xylose-fermenting oleaginous yeasts for lipid production in xylose solution. The two-stage culture method should provide a new opportunity to make the industrial production of biodiesel possible in the future.
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POLYMER BULLETIN
·
2015
·Vol. 72
·期 6
·1531-1543
·
IF 1.438
Zhou Zhihua,Huang Huihua,Huang Tianlong,Peng Cheng,Zhou Hu,Liu Qingquan,Zeng Wennan,Liu Lihua,Cao Dafu,He Siliang,Xiang Liujiao,Yan Hua
Novel maleated poly( d , l -lactide- co -glycolide) (MPLGA) was melt copolymerized directly from maleic anhydride, d,l -lactide and glycolide monomers with stannous octoate as catalyst and benzoyl peroxide as initiator. FTIR, 1 H NMR, GPC and maleation content were employed to qualitatively characterize the synthesized MPLGA. The effects of the catalyst dosage, reaction time and reaction temperature on weight average molecular mass, polydispersity index and maleation content of MPLGA were also investigated. The results indicated that the novel MPLGA was successfully obtained using melting-copolymerization method. MPLGA, with a weight average molecular mass of about 5.39 × 10 4 Da, a polydispersity index of 1.51 and a maleation content of 7.1 wt%, was obtained when polymerization was conducted with catalyst dosage of 0.03 wt% for 36 h at 140 °C. The results indicated that the introduction of maleic anhydride into PLGA may offer new and important options for biomaterial applications.
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RSC Advances
·
2015
·Vol. 5
·期 36
·28233-28241
·
IF 3.84
Yuhe Xiu,Angjun Chen,Xuerui Liu,Chen Chen,Jizhong Chen,Li Guo,Ran Zhang,Zhenshan Hou
Novel polymer-supported Brønsted acid polymer catalysts have been successfully synthesized by radical polymerization, followed by a simple solvothermal route and ion exchange step. The as-synthesized co-polymers were characterized by Elemental Analysis, FT-IR spectroscopy, thermogravimetric analysis and scanning electron microscopy and then were employed as a heterogeneous catalyst for dehydration of sorbitol. Especially, the polymer catalysts have a unique advantage of adjustable Brønsted acidity, and the polymer with 1.82 mmol g−1 of Brønsted acidity was a very efficient catalyst for highly selective dehydration of sorbitol into 1,4-anhydro-D-sorbitol. The dehydration reaction can be completed within 4 h and the selectivity of 1,4-anhydro-D-sorbitol achieved 90%. Moreover, the present catalysts resisted acid-leaching and can be recycled in five consecutive cycles without obvious loss of activity.
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Soft Matter
·
2015
·Vol. 11
·期 31
·6266-6274
·
IF 4.029
Chen Chen,Yue-Cheng Qian,Chuan-bin Sun,Xiao-Jun Huang
The amphiphilic random copolymer poly(β-D-glucose-co-1-octyl)phosphazene (PGOP) can undergo continuous morphological transitions in DMF–water mixed solvents. In this study, the ratio of glucose moieties to octyl moieties was controlled via a two-step thiol–ene reaction. As a result, polyphosphazenes with glycosyl functionalization degrees of 58.1% (PGOP-1), 74.1% (PGOP-2) and 87.0% (PGOP-3) were obtained. These amphiphilic polyphosphazenes self-assemble in both water and water–DMF mixtures. Several self-assembled morphologies including spheres, rods and vesicles were formed though careful control of the water content (WC) in the DMF solvent as well as of the hydrophilicity or hydrophobicity of the copolymers. We also found that an increase in the hydrophobic proportion led to faster morphological transitions at a constant WC. The thermodynamics of micellization were also studied by Isothermal Titration Calorimetry (ITC), and the strong hydrophobic interactions in PGOP-1 were demonstrated by their highly exothermic nature. These self-assemblies have potential applications in biosensing, lectin adsorption and drug loading with controlled release.
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TALANTA
·
2016
·
IF 4.035
Jun Fang Jiang,Juan Qiao,Xiao Yu Mu,Myeong Hee Moon,Li Qi
In this work, a unique D-amino acid oxidase reactor for enhanced enzymolysis efficiency is presented. A kind of magnetic polymer matrices, composed of iron oxide nanoparticles and porous polymer membrane (poly styrene-co-maleic anhydride), was prepared. With covalent bonding D-Amino acid oxidase on the surface of the matrices and characterization of scanning electron microscope and vibrating sample magnetometer, it demonstrated that the membrane enzyme reactor was successfully constructed. The enzymolysis efficiency of the enzyme reactor was evaluated and the apparent Michaelis-Menten constants of D-Amino acid oxidase were determined ( K m was 1.10 mM, V max was 23.8 mM min −1 ) by a chiral ligand exchange capillary electrophoresis protocol with methionine as the substrate. The results indicated that the enzyme reactor could exhibit good stability and excellent reusability. Importantly, because the enzyme and the substrate could be confined into the pores of the matrices, the enzyme reactor displayed the improved enzymolysis efficiency due to the confinement effect. Further, the prepared enzyme reactor was applied for D-Amino acid oxidase inhibitors screening. It has displayed that the proposed protocol could pave a new way for fabrication of novel porous polymer membrane based enzyme reactors to screen enzyme inhibitors .
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2016
·Vol. 34
·期 5
·563-577
·
IF 1.811
Dong Li-qi,Hu Du-fen,Duan Xue-min,Wang Zhi-peng,Zhang Kai-xin,Zhu Xiao-fei,Sun Hui,Zhang You-shan,Xu Jing-kun
Two pairs of amino-acid functionalized poly(3,4-ethylenedioxythiophene) (PEDOT) derivatives, namely, poly( N -(tert-butoxycarbonyl)- L -methionyl (3,4-ethylenedioxythiophene-2’-yl)methylamide) ( L -PEDOT-Boc-Met) and poly( N -(tert-butoxycarbonyl)- D -methionyl (3,4-ethylenedioxythiophene-2’-yl)methylamide) ( D -PEDOT-Boc-Met); poly( L -methionyl (3,4-ethylenedioxythiophene-2’-yl)methylamide) ( L -PEDOT-Met) and poly( D -methionyl (3,4-ethylenedioxythiophene-2’-yl)methylamide) ( D -PEDOT-Met) were synthesized via chemical oxidative polymerization of corresponding monomers. The structural characterization, spectroscopic properties and thermal stability of these monomers and polymers were systematically explored by FTIR spectra, Raman spectra, XRD spectra, UV-Vis spectra and thermogravimetric analysis. As chiral electrode materials, these polymers were employed to successfully recognize 3,4-dihydroxyphenylalanine (DOPA) enantiomers by cyclic voltammetry (CV) in sulphuric acid solution. The measurement results reveal that the tendency was hetero-chiral interaction between L -PEDOT-Met/PVA/GCE and D -DOPA, D -PEDOT-Met/PVA/GCE and L -DOPA, respectively. Also, the mechanism of chiral discrimination was discussed. All the results implied that the combination of electrochemical molecular recognition technology and chiral PEDOT materials can be a promising approach for chiral recognition and may open new opportunities for facile, biocompatible, sensitive and robust chiral assays in biochemical applications.
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JOURNAL OF MOLECULAR LIQUIDS
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2017
·
IF 3.648
Yonghong Hu,Yemin Yu,Yuhao Zhang,Wenge Yang,Tao Li,Renlun Deng,Min Jiang
The solid-liquid equilibrium (SLE) of d -sorbitol in (ethanol + acetonitrile) binary solvent mixtures was explored at temperatures ranging from 278.15 K to 333.15 K under 0.1 MPa. For the temperature range investigated, the equilibrium solubility varies with the composition of the solvent and temperature. The equilibrium solubility data were then modelled using modified Apelblat equation, a variant of the combined nearly ideal binary solvent/Redich-Kister (GSM) model and Jouyban-Acree model. Computational results showed that the modified Apelblat equation and CNIBS/R-K model have the low mean deviation ( MD ). In addition, the thermodynamic properties of the solution process, including the Gibbs energy , enthalpy, and entropy were calculated by the van't Hoff analysis.
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JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 23
·4653-4660
·
IF 2.557
Haoran Fan,Jinpeng Wang,Qingran Meng,Yaoqi Tian,Xueming Xu,Zhengyu Jin
Photoirradiation surface molecularly imprinted polymers for the separation of 6- O -α- d -maltosyl-β-cyclodextrin were synthesized using functionalized silica as a matrix, 4-(phenyldiazenyl)phenol as a light-sensitive monomer, and 6- O -α- d -maltosyl-β-cyclodextrin as a template. Fourier transform infrared spectroscopy results indicated that 4-(phenyldiazenyl)phenol was grafted onto the surface of functionalized silica. The obtained imprinted polymers exhibited specific recognition toward 6- O -α- d -maltosyl-β-cyclodextrin. Equilibrium binding experiments showed that the photoirradiation surface molecularly imprinted polymers obtained the maximum adsorption amount of 6- O -α- d -maltosyl-β-cyclodextrin at 20.5 mg/g. In binding kinetic experiments, the adsorption reached saturation within 2 h with binding capacity of 72.8%. The experimental results showed that the adsorption capacity and selectivity of imprinted polymers were effective for the separation of 6- O -α- d -maltosyl-β-cyclodextrin, indicating that imprinted polymers could be used to isolate 6- O -α- d -maltosyl-β-cyclodextrin from a conversion mixture containing β-cyclodextrin and maltose. The results showed that the imprinted polymers prepared by this method were very promising for the selective separation of 6- O -α- d -maltosyl-β-cyclodextrin.
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Journal of the Mechanical Behavior of Biomedical Materials
·
2017
·
IF 3.11
Guangmei Huang,Zhiyun Du,Zuoying Yuan,Lihua Gu,Qing Cai,Xiaoping Yang
Biodegradable organic-inorganic composites composed of polylactide (PLA) and hydroxyapatite (HA) are important bone repairing materials, while the dispersibility of nanoscaled HA in PLA and the interfacial adhesion between HA and PLA remained unsatisfactory. In this study, poly(D-lactide) (PDLA) oligomers with different molecular weights were grafted onto HA nanorods (HA-PDLA), and the HA-PDLA hybrids were mixed with poly(L-lactide) (PLLA). Dispersibility of HA-PDLA hybrids in PLLA matrix was investigated, and the formation of PDLA/PLLA stereocomplex at the interface of HA-PDLA and PLLA was studied by characterizations including crystallization, mechanical and thermal properties, taking PLLA grafted HA (HA-PLLA) and unmodified HA as comparisons. Surface grafting of PLLA or PDLA oligomers both significantly improved the dispersibility of HA nanorods in PLLA matrix. Benefiting from PDLA/PLLA stereocomplexation, HA-PDLA could increase the tensile strength and the elongation of resulting PLLA/HA nanocomposites more significantly than HA-PLLA. The interfacial interaction between HA-PDLA and PLLA matrix could be further strengthened by increasing the molecular weights of those grafted PDLA chains on HA, which was verified by changes in crystallization and glass transition behaviors. To sum up, the concept of using PDLA/PLLA stereocomplexation was feasible and effective in preparing PLA-based organic-inorganic nanocomposites targeting bone repairing.
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