CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Wenjin Li,Lei Wan,Yan Dong,Hang Luo,Wangzhe Li,Tin Pou Lai,Jian Zhao,Guozheng Liu,Yanxin Gao,Yida Deng,Wenbin Hu,Liyun Zhang
The coupling of photocatalytic assemblies with enzyme-catalyzed reactions offers a promising strategy for storing light energy in the form of chemical bonds, producing clean fuels and high-value chemicals. Such systems require high performance and robustness of all components, as well as effective coupling between inorganic and enzymatic modules to achieve efficient light harvesting and high product yields. In this work, a composite photocatalytic module, consisting of a heterostructure formed by silver nanoclusters (AgNCs) and graphitic carbon nitride (g-C 3 N 4 ), was coupled with an amino-containing Rh-based complex ([Cp*Rh(bpy(NH₂)₂)H₂O] 2+ ) for more efficient electron delivery and NADH recycling. The regenerated NADH was subsequently utilized by the enzymatic module, where the metal-organic framework (MOF) ZIF-90 was employed as a protective compartmentalization matrix for NADH-dependent L-glutamate dehydrogenase (GluDH). The resulting system achieved efficient conversion of NAD + to NADH, along with a notable L-glutamate production rate of 570 μmol g cat −1 h −1 , underscoring the potential of composite photosynthetic systems and paves the way for future improvements through enhanced integration and optimization of key photocatalytic modules.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2025
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IF 4.7
Guo-Xuan Liu,Fan Diao,Guang Lu,Qiang Zheng,Bin Fu,Hua-Chen Jiao,Ke-Zhou Wang,Dong-Hai Liu
Alectinib is the first-line therapy for anaplastic lymphoma kinase rearranged non-small cell lung cancer globally. Sinus bradycardia, as the major adverse cardiac events of alectinib, still widely impact patient’s quality of life. However, its underlying mechanism remains elusive. The aim of this study was to reveal the pathogenesis of the alectinib induced sinus bradycardia (AISB) in a rat model, including the electrophysiology alterations and the molecular mechanism. SD rats were administered alectinib (10 mg / kg / day) by gavage for 7 to 10 days to mimic the clinical AISB. 3-days alectinib treatment did not change heart rate and sinus node recovery time (SNRT) as assessed through in vivo electrophysiology study. Also, alectinib didn’t influence automaticity in isolated heart or single sinus node cardiomyocytes, indicating alectinib cannot decrease sinus node function rapidly. The decreased heart rate and prolonged SNRT was found after 7-days alectinib treatment. The inducibility of atrial fibrillation was not affected under the same condition. The RNA-seq assay revealed the transcriptomic alterations in sinus node of alectinib treated rats, and the dysregulation of genes in cardiac function were observed. The decreased expression of L-type calcium channel Cacna1d was confirmed among the channel candidates identified from RNA-seq assay. Subsequently, the patch-clamp test revealed the reduction in the corresponding L-type calcium current density in 7-days alectinib treated rats. These findings revealed that the AISB was caused by the reduction in Cacna1d expression which resulted in the electro-dysfunction mediated by the suppressed I CaL .
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Journal of the Taiwan Institute of Chemical Engineers
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2024
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IF 5.5
Xiao Duan,Qiang Wang,Wenfeng Che,Ting Li,Ke Zhang,Lingna Han,Lihua Song,Wei Guo
Background Doxorubicin (Dox) is a potent anticancer drug with severe side effects to normal tissues. To fabricate a nanomedicine with Dox is a better method to address the side effects of Dox in normal tissues. However, nanomedicines with unapproved materials could not be easily applied in clinic, hence, a nanomedicine fabricated by endogenous materials is a potential method to increase the possibility of clinical application. Methods In this study, a redox-responsive nanomedicine with clinical-applied anticancer of Dox and endogenous molecule of l -glutathione oxidized (GSSG) was prepared for cancer therapy. : The carboxyl and amino groups in l -glutathione oxidized can be reacted itself to form the poly- l -glutathione oxidized peptide-like polymer. Meanwhile, the Dox were conjugated in poly- l -glutathione oxidized polymer by amnio group in Dox and the structure of Dox-conjugated poly- l -glutathione oxidized polymer (Dox-Poly GSSG) was confirmed by FT-IR, NMR and MALDIT-TOF. Afterward, the drug loading of Dox (22 %) in poly-GSSG was measured by UV–vis and the accumulative Dox release rate of Dox-Poly GSSG is 46 % in 5 mg/mL reduced glutathione (GSH) environment at 72 h The size stability of Dox-Poly GSSG in PBS and PBS with GSH was evaluated by dynamic laser scatter (DLS) and transmit emission microscopy (TEM). Furthermore, cell viabilities of Dox-Poly GSSG were similar with free Dox evaluated by CCK-8 assay and flow cytometry experiment, respectively. The distribution of Dox-Poly GSSG in nuclei and tumor tissues was evaluated by Confocal laser scanning microscope and in vivo imaging system, respectively. Significant Findings The drug release results showed that the Dox could be controllable released in high concentration of GSH in tumor tissues. The tumor inhibition, H&E and TUNEL experiments showed that the Dox-Poly GSSG group can significantly inhibit the tumor growth compared to saline group and avoid major side effects to normal tissues compared to free Dox. This redox-responsive nanomedicine with clinical-applied anticancer of Dox and endogenous molecule of GSSG is a potential candidate for clinical application.
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Biology-Basel
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2025
·Vol. 14
·期 6
·589
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IF 3.5
Xin Yang,Canhui Hong,Tangfei Guan,Chenggui Zhang,Yongshou Yang,Peiyun Xiao,Huai Xiao,Zhengchun He
Simple SummaryIschemic stroke (IS) is a high-mortality cardiovascular disease. This study analyzedPeriplaneta americana(L.) extract (PAS840) components using LC-MS/MS and peptidomics, and predicted its IS-related therapeutic targets by network pharmacology. In BV-2 cells after OGD/R injury, multiple techniques explored PAS840’s effects on cell functions and pathways. In a tMCAO rat model, various methods evaluated its impact on the blood–brain barrier, inflammation, and neural function. Results show PAS840 can act on multiple IS-related pathways. It inhibits the NLRP3 inflammasome pathway in BV-2 cells, reduces oxidative stress, inflammation, and pyroptosis, and improves cell viability. In tMCAO rats, it can reduce the NLRP3 inflammasome pathway, activate growth factor pathways, and protect the blood–brain barrier. These findings suggest that PAS840 is a promising drug worthy of development for the treatment of IS.Ischemic stroke (IS) is a high-mortality, multi-complication cardiovascular disease. Reducing brain injury and promoting neuronal repair after IS onset remain important challenges for current treatments. Our team previously found that PAS840, an extract fromPeriplaneta americana(L.), protects nerve function; this study further uses LC-MS/MS and peptidomics to analyze PAS840’s components and network pharmacology to predict its ischemic stroke (IS) therapeutic targets. We then employed Transwell, a biochemical kit, real-time quantitative polymerase chain reaction (RT-qPCR), and transcriptomics to investigate PAS840’s effects on migration ability, oxidative stress levels, and cellular pathways in mouse microglial cells (BV-2) following oxygen–glucose deprivation/reoxygenation (OGD/R) injury. Finally, using Evans blue staining, immunohistochemical analysis, and RT-qPCR, we investigated PAS840’s effects on the blood–brain barrier, inflammation pathways, and neural function in a transient middle cerebral artery occlusion (tMCAO) rat model. PAS840 components target multiple IS pathways, effectively inhibit NF-κB/NLRP3/Caspase-1/IL-1β inflammasome pathway activation in BV-2 cells following OGD/R, reduce cellular oxidative stress, inflammation, and pyroptosis, and improve cell viability and migration ability. PAS840 decreases NF-κB/NLRP3/Caspase-1/IL-1β inflammasome pathway expression in tMCAO rat brains, reduces inflammation, activates BDNF/VGF/NGR1/Erbb4 neurotrophic factor and vascular endothelial growth factor pathways, enhances neuronal cell viability, and effectively protects and repairs the blood–brain barrier.
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ACS Applied Nano Materials
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2025
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IF 5.5
Jing Hu,Shaogui Wu
A facile solvothermal strategy employing l-asparagine as the sole carbon–nitrogen precursor was developed for the synthesis of bright blue-emitting carbon dots (B-CDs) with remarkable optical properties. The as-prepared B-CDs exhibit optimal excitation/emission wavelengths at 378 and 466 nm, a notably high quantum yield (QY) of 19.2%, and superior aqueous dispersibility, salt tolerance, and photostability. Leveraging their ultrasensitive response toward tetracycline antibiotics (TET, CTC, and OTC), we engineered a fluorescent nanoprobe enabling quantitative detection with an impressive linear correlation coefficient (R2) of 0.999 and a detection limit down to 0.139 μmol/L. Furthermore, preliminary investigations revealed the exceptional potential of B-CDs as functional materials in memristive devices, demonstrating their unique advantages for advanced electronic applications. This study not only expands the multifunctionality of carbon-based nanomaterials but also provides innovative material platforms for antibiotic surveillance and neuromorphic computing technology.
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PLANT AND SOIL
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2025
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IF 4.1
Yi Yuying,Li Wenhui,Wang Jinzhao,Sun Guozhi,Ma Ying,Li Fali,Shi Wei,Hu Yanfeng,Jia Honglei,Li Jisheng
Aims Hydrogen sulfide (H₂S), a gaseous signaling molecule, plays a pivotal role in facilitating plant resistance to cadmium (Cd) stress. Glutathione S-transferases (GSTs) are critical enzymes to tolerate Cd stress in plants. However, the regulatory interplay between H₂S and GSTs activity remains elusive. Here, we identified the persulfidation of SlGSTU24 as a previously uncharacterized mechanism regulating Cd detoxification. Methods To investigate the effect of H₂S on the growth of tomato seedlings under Cd stress, 1, 3, and 5 mg/L CdCl 2 and 200 μM NaHS (an H₂S donor) were used to treat three-week-old seedlings for 7 days in this study. Morphological characteristics, malondialdehyde (MDA) content, non-protein thiol (NPT) levels, metallothioneins (MTs) and protein content of treated seedlings were analyzed. GSTs activity were assessed, and persulfidation proteomics was conducted on GSTs. Mass spectrometry and the modified biotin switch method (MBSM) identified persulfidated residues in SlGSTU24. Cys132 was mutated to alanine for functional analysis. Results Cd exposure inhibited the growth of tomato seedlings, while treatment with NaHS alleviated growth inhibition, decreased malondialdehyde (MDA) content and electrolyte leakage (EL) under Cd stress. The application of NaHS increased non-protein thiols glutathione (GSH) and phytochelatins (PCs) contents, and protein thiols metallothionein (MTs) content. The endogenous activity of GSTs was enhanced by NaHS. Cys132 in SlGSTU24 was identified as a persulfidated residue. Persulfidation of Cys132 increased the SlGSTU24 wild type (WT) activity. When Cys132 was mutated to alanine, H₂S-induced SlGSTU24 activity were compromised in SlGSTU24 Cys(C)132 Ala(A) . Conclusions H₂S-mediated persulfidation of Cys132 in SlGSTU24 enhances its activity and improves tomato resistance to Cd stress.
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Acta Biomaterialia
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2021
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IF 8.947
Xiaomeng Ren,Ningning Wang,Yaxin Zhou,Aixin Song,Guoxia Jin,Zhonghao Li,Yuxia Luan
Arginase 1 (ARG1) inactivates T cells by degrading L-arginine, severely reducing the immunotherapeutic efficacy. Effectively blocking the ARG1 pathway remains a challenge. L-norvaline is a very cheap and negligible side effects inhibitor of ARG1. However, its blockage efficacy for ARG1 is impeded by its high half-maximal-inhibitory concentration (IC50) requiring high drug loading content of L-norvaline in carriers. Moreover its high water solubility results in bursting and uncontrolled release. Herein we reported an injectable hydrogel strategy via an L-norvaline-based immunomodulating gelator that could effectively block ARG1 pathway. The designed gelator was a diblock copolymer containing L-norvaline-based polypeptide block, which could construct a thermally responsive injectable hydrogel by its self-gelation in tumor microenvironments. The hydrogel not only ensures high drug loading of L-norvaline, but also ensures controlled release of L-norvaline through responsive peptide bond cleavage, thereby solving the problems encountered by L-norvaline. The injectable hydrogel in combination with doxorubicin hydrochloride demonstrated a potent immunotherapy for removal of primary tumors, suppression of abscopal tumors and inhibition of pulmonary metastasis by combining the blockage of ARG1 pathway and the immunogenic cell death. Our immunomodulating gelator strategy provides a robust injectable hydrogel platform to efficiently reverse ARG1 immunosuppressive environments for amplified immunotherapy. Statement of Significance We designed an injectable hydrogel via an L-norvaline-based immunomodulating gelator. The designed gelator, a diblock copolymer containing an L-norvaline-based polypeptide block, enabled a thermally responsive injectable hydrogel by its self-gelation in tumor microenvironments. The injectable hydrogel not only guarantees high drug loading of L-norvaline, but also ensures controlled release of L-norvaline through responsive peptide bonds cleavage, thereby solving the problems encountered by L-norvaline. By further introducing doxorubicin hydrochloride in the hydrogel for inducing immunogenic cell death, the hydrogel showed remarkable immunotherapeutic efficacy towards ablation of primary tumors, suppression of abscopal tumors and inhibition of pulmonary metastasis. Our immunomodulating gelator strategy provides a new concept to efficiently reverse Arginase 1 immunosuppressive environments for amplified immunotherapy.
研究领域:
生物医学工程
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肿瘤学
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免疫学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2014
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IF 3.107
Yi Liu,Sha Li,Hong Xu,Lingtian Wu,Zheng Xu,Jing Liu,Xiaohai Feng
d-Tagatose, a functional sweetener, is commonly transformed from d-galactose by l-arabinose isomerase (l-AI). In this study, a novel type of biocatalyst, l-AI from Lactobacillus fermentum CGMCC2921 displayed on the spore surface of Bacillus subtilis 168, was developed for producing d-tagatose. The anchored l-AI, exhibiting the relatively high bioactivity, suggested that the surface display system using CotX as the anchoring protein was successfully constructed. The stability of the anchored l-AI was significantly improved. Specifically, the consolidation of thermal stability representing 87% of relative activity was retained even at 80 °C for 30 min, which remarkably favored the production of d-tagatose. Under the optimal conditions, the robust spores can convert 75% d-galactose (100 g/L) into d-tagatose after 24 h, and the conversion rate remained at 56% at the third cycle. Therefore, this biocatalysis system, which could express the target enzyme on the food-grade vector, was an alternative method for the value-added production of d-tagatose.
研究领域:
生物工程
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食品科学
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MICROCHIMICA ACTA
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2018
·Vol. 185
·期 4
·1-9
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IF 5.705
Song Jinyi,Yang Chengcheng,Ma Jiao,Han Qian,Ran Peiyao,Fu Yingzi
An electrochemical chiral multilayer nanocomposite was prepared by modifying a glassy carbon electrode (GCE) via opposite-charge adsorption of amino-modified β-cyclodextrin (NH 2 -β-CD), gold-platinum core-shell microspheres (Au@Pts), polyethyleneimine (PEI), and multi-walled carbon nanotubes (MWCNTs). The modified GCE was applied to the enantioselective voltammetric determination of tryprophan (Trp). The Au@Pts enable an effective immobilization of the chiral selector (NH 2 -β-CD) and enhance the electrochemical performance. Scanning electron microscopy, transmission electron microscopy, UV-vis spectroscopy, FTIR and electrochemical methods were used to characterize the nanocomposite. Trp enantiomers were then determined by differential pulse voltammetry (DPV) (with a peak potential of +0.7 V vs. Ag/AgCl). The recognition efficiency was expressed by an increase in peak height by about 32% for DPV determinations of L-Trp compared to D-Trp in case of a 5 mM Trp solution of pH 7.0. Response was linear in the 10 μM to 5.0 mM concentration range, and the limits of detection were 4.3 μM and 5.6 μM with electrochemical sensitivity of 43.5 μA·μM −1 ·cm −2 and 34.6 μA·μM −1 ·cm −2 for L-Trp and D-Trp, respectively (at S/ N = 3).
Schematic of an electrochemical chiral multilayer nanocomposite composed of multi-walled carbon nanotubes (MWCNTs), polyethyleneimine (PEI), gold-platinum core-shell microspheres (Au@Pt) and amino-modified β-cyclodextrin (NH 2 -β-CD). It was prepared by modifying a glassy carbon electrode (GCE) for enantioselective voltammetric determination of tryptophan (Trp) enantiomers.
研究领域:
分析化学
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生物化学
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TALANTA
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2020
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IF 5.339
Fangbin Fan,Licheng Wang,Yijing Li,Xusheng Wang,Xiaofeng Lu,Yong Guo
l -Cysteine (L-Cys) and N-Isopropyl acrylamide (NIPAM)-modified silica spheres as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC) was produced firstly by cross-linking polymerization. Some characterizations in this article confirmed that the synthesis of Cys-Si-NIPAM is successful. Some polymer layers can be observed through transmission electron microscopy (TEM). In addition, through nitrogen adsorption porosity method, scanning electron microscopy (SEM), thermal gravimetric analysis (TGA) and other characterization methods, we can find the significant changes after modify. It has good hydrophilic property and higher column effect than bare silica column, Si-Cys column and Si-NIPAM column under the same conditions. It has good separation effect for some hydrophilic analytes such as 5 nucleosides and nucleoside bases, 5 amino acids , 4 sulfonamide drugs and 10 saccharides . The effects including column temperature, pH and organic solvent content on chromatographic performance were studied, which proved that hydrophilic interactions can be simultaneously existed between the stationary phase and the analytes. In addition, reproducibility and efficiency of the Cys-Si-NIPAM column was also investigated, the results illustrated that the stationary phase have passable stability (the intraday RSDs 0.08–0.44%, n = 3 and the interday RSD 0.46–3.50%, n = 3) and ideal efficiency (plates per meter, ~45700 plates/m). In conclusion, the preparation process of this hydrophilic liquid chromatography stationary phase is not only simple, but also can meet the basic requirements for the separation of hydrophilic analytes.
研究领域:
分析化学
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色谱技术
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高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2017
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IF 1.174
Lei Guo,Yangdong Hu,Lianying Wu,Chen Liang,Weitao Zhang
To achieve green hydrolysis technology of hemicellulose through repeated using hydrolysate, the hydrolysis of hemicellulose in corncob was studied. The influence of repeated use of corncob hydrolysate on concentrations of d -xylose and l -arabinose was investigated. The loss rates of d -xylose in the prepared d -xylose solutions both with and without corncob, and repeated using corncob hydrolysate under identical acidity condition were discussed. The result shows that d -xylose concentration and l -arabinose concentration are all gradually increasing with the growing time of repeated use of corncob hydrolysate. After the fifth repetition, the concentrations of d -xylose and l -arabinose are 196.7 g·L − 1 and 22.0 g·L − 1 , respectively. Substance inhibiting the degradation of d -xylose is generated during repeated use of corncob hydrolysate, and the production is further proved by the change of d -xylose concentration and the loss rate of d -xylose over heating time.
研究领域:
生物技术
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化学工程
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可持续化学
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JOURNAL OF APPLIED ELECTROCHEMISTRY
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2019
·Vol. 49
·期 4
·413-421
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IF 2.366
Duan Wanyi,Li Aoqi,Chen Yujuan,Zhang Jiahui,Zhuo Kelei
In this work, we prepared reduced graphene oxide-supported PtCo nanospheres (PtCo/rGO) by a facile one-step hydrothermal method with the assistance of l -proline as the shape-directing agent. We systematically investigated the effects of various experimental conditions on the morphology and structure of the as-prepared PtCo/rGO hybrids. Compared with commercial Pt/C (10 wt%) and Pt/rGO catalysts, the as-prepared PtCo/rGO has better catalytic activity and poisoning resistance toward methanol electrooxidation and can be used as a promising catalyst for direct methanol fuel cells. Graphical abstract The rGO-supported PtCo nanosphere hybrids are successfully synthesized by a facile one-pot hydrothermal method with the assistance of l -proline. l -proline acts as shape-controlled agent for synthesizing PtCo/rGO. The as-prepared PtCo/rGO hybrids exhibit a higher electrocatalytic activity and poisoning-resistant ability for methanol oxidation in acidic media.
研究领域:
材料科学
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电化学
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能源科学
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高影响因子
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