PHARMACEUTICAL BIOLOGY
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2023
·
IF 3.8
Shouhai Wu,Meixia Yan,Junyi Liu,Yizhen Li,Ruimin Tian,Chuang Li,Lihuang Huang,Zhisheng Lu,Peng Xu,Wei Mao
Context Clerodendranthus spicatus Thunb. (Labiatae) (CS), a perennial traditional Chinese medicinal herb that can reduce serum uric acid (sUA) levels and ameliorate renal function is widely used to treat hyperuricaemic nephropathy (HN).Objective To investigate the molecular mechanism of action of CS in HN treatment using in vivo and in vitro experiments.Materials and methods Sprague-Dawley rats were randomly divided into control, HN, CS and positive control allopurinol groups. The HN group was intraperitoneally injected with 750 mg/kg oxonic acid potassium (OA), whereas the CS group was injected with OA along with a gavage of CS (low dose 3.125 g/kg, high dose 6.25 g/kg) for five weeks. For in vitro studies, uric acid-treated HK2 cells were used to verify the therapeutic mechanism of CS in HN.Results HN rats exhibit pathological phenotypes of elevated sUA levels and renal injury. CS significantly improved these symptoms and sUA (p < 0.05) and blood urea nitrogen (p < 0.01) levels, and dramatically improved renal tubular injury in HN rats. The IC50 value of UA (uric acid) in HK2 cells was 826.32 ± 3.55 μg/mL; however, 120 ng/mL CS had no significant cytotoxicity on HK2 cells. In vivo and in vitro studies showed that CS inhibited NF-κB phosphorylation and inhibited α-smooth muscle actin (α-SMA) and vimentin expression while increasing E-cadherin expression, suggesting that CS inhibited the fibrotic process in renal cells, thus protecting renal function.Discussion and conclusions These findings provide a fundamental understanding of the application of CS in HN treatment to better guide clinical interventions.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2023
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IF 3.1
Chan-juan Chai,Yao Sun,Rui-fang Chi,Hui-yu Yang,Bin Yang,Bao Li
Cardiac remodeling (CR), characterized by cardiac hypertrophy and fibrosis, leads to the development and progression of heart failure (HF). Nowadays, emerging evidence implicated that inflammation plays a vital role in the pathogenesis of CR and HF. Astragaloside IV (AS-IV), an effective component of Astragalus membranaceus , exerts cardio-protective and anti-inflammatory effects, but the underlying mechanism remains not fully elucidated. This present study aimed to investigate the effects of AS-IV on cardiac hypertrophy and fibrosis in cultured H9C2 cells stimulated with LPS, as well as explore its underlying mechanisms. As a result, we found AS-IV could reduce the cell surface size, ameliorate cardiac hypertrophy and fibrosis in LPS-induced H9C2 cells. To specify which molecules or signaling pathways play key roles in the process, RNA-seq analysis was performed. After analyzing the transcriptome data, CCL2 has captured our attention, of which expression was sharply increased in model group and reversed by AS-IV treatment. The results also indicated that AS-IV could ameliorate the inflammatory response by down-regulating NF-κB signaling pathway. Additionally, a classical inhibitor of CCL2 (bindarit) were used to further explore whether the anti-inflammatory effect of AS-IV was dependent on this chemokine . Our results indicated that AS-IV could exert a potent inhibitory effect on CCL2 expression and down-regulated NF-κB signaling pathway in a CCL2-dependent manner. These findings provided a scientific basis for promoting the treatment of HF with AS-IV.
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ELECTROCHIMICA ACTA
·
2023
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IF 6.6
Sirong Li,Yipeng Zhang,Xin Yu,Zhenlong Wang,Guofei Zhang,Zhengyi Zhao,Zhiyong Yan,Xuechun Xiao
An environmentally friendly method of producing hydrogen is electrocatalytic water splitting, but the high theoretical potential of the anode's OER (1.23 V vs. RHE) restricts the efficiency of hydrogen production . Therefore, using the urea oxidation reaction (UOR, theoretical potential of 0.37 V vs. RHE) in place of the OER can significantly increase the efficiency of hydrogen production. Here, we grow the hydroxide layer of Ni-Co-Fe directly on NF by one-step electrodeposition and obtain the best catalyst by regulating the elemental ratio of Co and Fe. Through tests, it is found that NiCoFe(OH) x /NF(Co: Fe=3: 1) exhibits the best electrocatalytic performance, and the required potential is only 1.24 and 1.30 V when the current density reaches 10 and 50 mA cm −2 in the UOR process, which is 170 and 140 mV lower than the OER overpotential under the same conditions, showing a more advantageous anodic catalytic effect. At the same time, integral water decomposition in an electrolyte of 1 M KOH adds with 0.5 M urea also shows more favorable catalytic activity and stability. The NiCoFe(OH) x /NF(Co: Fe=3: 1) plays an important role in improving the efficiency of hydrogen production by water splitting through simple electrodeposition, and has sustainable development prospects.
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Foods
·
2023
·Vol. 12
·期 11
·2120
·
IF 5.2
Jie Li,Bingyong Mao,Xin Tang,Qiuxiang Zhang,Jianxin Zhao,Hao Zhang,Shumao Cui
Naringenin and apigenin are common flavonoids derived from edible plants with the potential to alleviate inflammation and improve skin antioxidation. This study aimed to evaluate the effects of naringenin and apigenin on oleic acid-induced skin damage in mice and compare their underlying mechanisms of action. Triglycerides and non-esterified fatty acids were significantly decreased by naringenin and apigenin, while apigenin intervention resulted in a better recovery of skin lesions. Naringenin and apigenin improved the antioxidative abilities of the skin by increasing catalase and total antioxidant capacity levels and decreasing malondialdehyde and lipid peroxide levels. The release of skin proinflammatory cytokines, such as interleukin (IL)-6, IL-1β, and tumor necrosis factor α, was inhibited after naringenin and apigenin pretreatments, but naringenin only promoted the excretion of IL-10. Additionally, naringenin and apigenin regulated antioxidant defense and inflammatory response by activating nuclear factor erythroid-2 related factor 2-dependent mechanisms and suppressing the expression of nuclear factor-kappa B. In summary, naringenin and apigenin are prospective ingredients that contribute to the amelioration of skin damage by activating anti-inflammatory and antioxidative responses.Keywords:naringenin;apigenin;antioxidative system;inflammation;signaling pathway
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2023
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IF 4.2
Chenyu Zhu,Xue Zhang,Fuzhi Li,Ruochen Yang,Xuan Zhao
Thin-film composite (TFC) nanofiltration (NF) has been widely utilized to separate monovalent ions from multivalent ions in industrial wastewater treatment. In this study, the pore properties of polyvinylidene fluoride (PVDF) substrates were adjusted by varying the content and molecular weights (MWs) of porogen polyethylene glycol (PEG). The TFC membranes fabricated on different substrates showed distinguishable permeance and selectivity. The PVDF membranes with 2 kDa PEG of 0.5–3.0 wt % and 20 kDa PEG of 0.2 wt % were fit for the substrates of the TFC NF membrane. These substrates are usually characterized by evenly distributed surface pores with porosity of 7–12% and tiny finger-like pores of less than 15 μm length. Such pore structures in the substrates are fit for the storage and migration of amine monomers, resulting in a defect-free PA layer. In a 6 h filtration of mining wastewater using an optimized TFC membrane, the permeance remained around 5 LMH/bar and the separation factor of SO42–/Cl– stayed over 13.39 with a maximum value over 30. This study shed light on improving NF membrane performance by simply adjusting the porogen in the substrate casting solution.
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RSC Advances
·
2023
·Vol. 13
·期 24
·16536-16548
·
IF 3.9
Hai-Yang Zhu,Ying-Hui Liu,Hai-Yun Sun,Dan-Dan Cao,Yu-Chuan Li,Si-Ping Pang
The development of environment-friendly and non-toxic green energetic materials and their safe, environmentally friendly, and economical production is very important to the national economy and national security. As an innovative, efficient, and environmentally friendly energetic material, the preferred preparation method of ammonium dinitramide (ADN) is the nitro-sulfur mixed acid method, which has the advantages of high yield, simple method, and easy access to raw materials. However, the large number of inorganic salt ions introduced by this method limits the large-scale production of ADN. Nanofiltration (NF) has been widely used in various industrial processes as a separation method with high separation efficiency and simple operation. In this study, NF was used for the desalination and purification of ADN synthesized by the mixed acid method. The effects of NF types, operation process (pressure, temperature, and feed solution concentration) on desalination efficiency, and membrane flux during purification were examined. The results showed that 600D NF could achieve the efficient desalination and purification of ADN. It was verified that the highest desalination and purification efficiency was achieved at 2 MPa pressure, 25 °C, and 1 time dilution of the feed solution, and the membrane flux of the desalination and purification process was stable. Under the optimized process conditions, the removal rate of inorganic salts and other impurities reached 99% (which can be recycled), the purity of ADN reached 99.8%, and the recovery rate reached 99%. This process has the potential for the large-scale production of ADN and provides a new process for the safe, efficient, and cheap preparation of energetic materials.
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MOLECULES
·
2023
·Vol. 28
·期 8
·3395
·
IF 4.6
Yi Ou,Zonglin You,Min Yao,Yingfan Cao,Xiu Xue,Min Chen,Rihui Wu,Lishe Gan,Dongli Li,Panpan Wu,Xuetao Xu,Wingleung Wong,Vincent Kam Wai Wong,Wenfeng Liu,Jiming Ye,Jingwei Jin
Naproxen is widely used for anti-inflammatory treatment but it can lead to serious side effects. To improve the anti-inflammatory activity and safety, a novel naproxen derivative containing cinnamic acid (NDC) was synthesized and used in combination with resveratrol. The results showed that the combination of NDC and resveratrol at different ratios have a synergistic anti-inflammatory efficacy in RAW264.7 macrophage cells. It was indicated that the combination of NDC and resveratrol at a ratio of 2:1 significantly inhibited the expression of carbon monoxide (NO), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), induced nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) and reactive oxygen species (ROS) without detectable side effects on cell viability. Further studies revealed that these anti-inflammatory effects were mediated by the activation of nuclear factor kappa-B (NF-κB), mitogen-activated protein kinase (MAPK) and phosphoinositide-3 kinase (PI3K)/protein kinase B (Akt) signaling pathways, respectively. Taken together, these results highlighted the synergistic NDC and resveratrol anti-inflammatory activity that could be further explored as a strategy for the treatment of inflammatory disease with an improved safety profile.Keywords:naproxen derivative;resveratrol;synergistic;anti-inflammatory;mechanism
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Yu Li,Jiangnan Li,Deliang Zhu,Guangcun Qian,Hongyan Tang
During water treatment, the chlorination degradation by active chlorine and membrane fouling seriously threaten the service life of nanofiltration (NF) membranes. In this work, dual-functionalized NF membranes were facilely fabricated by in-situ grafting of zwitterions, which were generated by in-situ grafting 2,4,6-Triaminopyrimidine (TAP) onto the separation layer and the subsequential quaternization. The introduction of zwitterions was confirmed by ATR-FTIR and XPS. Under constant chlorine exposure (24000 ppm·h), chlorine with high concentration proved to be more corrosive. After harsh 24000 ppm·h chlorination in different environments (pH = 4.0, 7.0, and 10.0, respectively), all the salt rejections of the zwitterion-grafted NF membranes (the QTAP NF membranes) were higher than 98.0% and barely decreased. The flux of the QTAP NF membranes was 23.5% higher than that of the pristine NF membranes in acid chlorine environment after chlorination. The SEM and ATR-FTIR results indicated that no obvious change occurred to the surface morphologies and chemical structures for the QTAP NF membranes after chlorination. Flux recovery ratio (Bovine serum albumin) of the QTAP NF membranes was 91.2%, which indicated good antifouling property. The quaternization process slightly increased the rejection for inorganic salts. The zwitterions endowed the membranes with excellent chlorine resistance and good antifouling property. This study could provide a facile and effective strategy for constructing dual-functional NF membranes with chlorine-resistance and antifouling property.
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Frontiers in Microbiology
·
2023
·
IF 5.2
Zhou Ziyuan,Wang Baohong,Pan Xiaxia,Lv Jiawen,Lou Zhuoqi,Han Yuqiu,Yao Yuanyuan,Chen Jun,Wang Qiangqiang,Li Lanjuan
Introduction: Acute liver failure (ALF) is a clinical condition with many causes, fast progression, and a poor prognosis. Previous research has indicated that microbial factors have a role in ALF, but a clear picture has yet to emerge.Methods: To investigate the specific involvement of microbial metabolites in ALF development, we pretreated D-GalN/LPS-induced ALF mice with indole derivatives, an influential class of gut microbial metabolites.Results: Contrary to their typical role as anti-inflammatory agents in the host, indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), and indolepropionic acid (IPA) gavage sensitize mice to D-GalN/LPS-induced-ALF with a rapid rise in serum transaminases and histologic lesion. For a clearer picture, we performed comprehensive analysis for the IAA therapy. IAA markedly amplified inflammatory response and cellular damage. The transcriptome analysis indicated the participation of the TNF-α/NF-κB signaling pathway. The structure of gut microbiota in ileum and the expression of Toll-like receptor 2 (Tlr2) in the liver were also significantly changed.Discussion: In conclusion, IAA pretreatment can exacerbate D-GalN/LPS-induced ALF via probable Tlr2/NF-κB pathway involvement and ileac dysbiosis characterized by enriched gram-positive genus with potential pathogenesis. Microbial metabolites IAA may aggravate individual susceptibility to D-GalN/LPS-induced ALF. Further investigation of the underlying mechanism is needed, and intervention with indole derivatives and related commensal species should be undertaken with caution.
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FOOD AND CHEMICAL TOXICOLOGY
·
2018
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IF 3.977
Jian Fang,Qiuyan Chen,Baozhu He,Jiaxuan Cai,Yawen Yao,Yi Cai,Suowen Xu,Kannan R.R. Rengasamy,Shanmugaraj Gowrishankar,Shunmugiah Karutha Pandian,Tiansheng Cao
Tanshinone IIA is one of the most predominant bioactive constituents of Danshen , a traditional Chinese medicinal plant with multiple cardiovascular protective actions. Although Tanshinone IIA has been well documented for its endothelial protective efficacy, studies unveiling the mechanism and/or molecular targets for its pharmacological activity are still inadequate. In recent studies, it has been envisaged that the expression of pentraxin 3 (PTX3) was associated with atherosclerotic cardiovascular diseases (ACVD). Therefore, the current study was designed to evaluate the possible role of Tanshinone IIA in influencing the expression of PTX3 in endothelial cells and thereby prevents endothelial dysfunction . Molecular analyses through real-time PCR, western blot , and ELISA revealed that Tanshinone IIA down-regulates PTX3 gene expression as well as protein secretion in human endothelial cells in the presence or absence of TNF-α. Besides, Tanshinone IIA inhibits the adhesion of THP1 cells (a monocytic cell line) to activated-endothelial cells stimulated with TNF-α. Furthermore, mechanistic studies uncovered the role of p38 MAPK/NF-κB pathway in Tanshinone II-A mediated pharmacological effects. Thus, the present study exemplifies the manifestation of Tanshinone IIA as a plausible alternative natural remedy for ACVD by targeting PTX3.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2019
·
IF 3.361
Sen Yang,Ge Kuang,Rong Jiang,Shengwang Wu,Tao Zeng,Yuanyuan Wang,Fangzhi Xu,Lingyi Xiong,Xia Gong,Jingyuan Wan
Acetaminophen (APAP) is a widely used over-the-counter drug for antipyretic and analgesic , but an overdose will induce acute liver injury. APAP hepatotoxicity has been the most common cause of acute liver failure in western countries with high morbidity and mortality. Geniposide (GP), an iridoid glycoside extracted from the fruit of Gardenia jasminoides , has been reported to exert a profound anti-inflammatory activity on acute and chronic diseases. However, it is never demonstrated whether GP can protect hepatocytes from APAP hepatotoxicity. In this study, we investigated the protective effect and underlying mechanism of GP against AILI. The results showed that GP pretreatment reduced the levels of ALT and AST in a dose-dependent manner and alleviated hepatocyte necrosis and apoptosis in mice exposed at APAP. Moreover, it suppressed the expression of CYP 2E1 and attenuated the exhaustion of GSH and accumulation of MDA in the liver. Furthermore, GP remarkably inhibited inflammatory cells infiltration and mitigated the release of IL-1β and TNF-α, and inhibited Toll-like receptor 4 (TLR4) expression and nuclear factor kappa (NF-κB) activation. These data suggested that GP could effectively protect hepatocytes from APAP hepatotoxicity through the down-regulation of CYP 2E1 expression and the inhibition of TLR4/NF-κB signaling pathway .
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PLoS One
·
2015
·Vol. 10
·期 2
·e0116879
·
IF 3.234
Li Zhang,Yuanyuan Li,Zhiwen Gu,Yuyue Wang,Mei Shi,Yun Ji,Jing Sun,Xiaopeng Xu,Lirong Zhang,Jingtin Jiang,Weifeng Shi
Polydatin and resveratrol, as major active components in Polygonum cuspidatum , have anti-inflammatory, antioxidant and antitumor functions. However, the effect and mechanism of polydatin and resveratrol on enterovirus 71 (EV71) have not been reported. In this study, resveratrol revealed strong antiviral activity on EV71, while polydatin had weak effect. Neither polydatin nor resveratrol exhibited influence on viral attachment. Resveratrol could effectively inhibit the synthesis of EV71/VP1 and the phosphorylation of IKKα, IKKβ, IKKγ, IKBα, NF-κB p50 and NF-κB p65, respectively. Meanwhile, the remarkably increased secretion of IL-6 and TNF-α in EV71-infected rhabdosarcoma (RD) cells could be blocked by resveratrol. These results demonstrated that resveratrol inhibited EV71 replication and cytokine secretion in EV71-infected RD cells through blocking IKKs/NF-κB signaling pathway. Thus, resveratrol may have potent antiviral effect on EV71 infection.
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