BMC Chemistry
·
2025
·Vol. 19
·期 1
·223
·
IF 4.6
Yuan Ming,Han Xiaofang,Zhang Zekai,Wang Ruijia,Guo Shengrong
In this study, organic halides, such as 2-bromobutane (C 4 H 9 Br), ethyl α-bromophenylacetate (BPA), ethyl 2-bromoisobutyrate (EBiB), and ethyl 2-bromopropionate (EBP) are utilized in conjunction with copper nanopowder (Nano-Cu) to initiate the polymerization of methyl methacrylate (MMA). Among these, BPA combined with nano-Cu exhibits the highest reactivity, resulting in the production of poly(methyl methacrylate) (PMMA) with a number-average molecular weight ( M n ) of 1.91 × 10 6 Da, a weight-average molecular weight ( M w ) of 3.46 × 10 6 Da, and a polydispersity index (PDI) of 1.81. A kinetic analysis of the polymerization reveals that the reaction orders for MMA, BPA, and nano-Cu concentration are 0.76, 0.49, and 0.77, respectively. The activation energy of the polymerization of MMA initiated by BPA is calculated to be 59.6 kJ/mol. The molecular weight of PMMA product is determined using gel permeation chromatography (GPC), while the structure of the synthesized PMMA is characterized through proton nuclear magnetic resonance ( 1 H NMR). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) is employed to analyze the precise structure and molecular weight of PMMA. Additionally, the nano-Cu recovered after the polymerization is examined using X-ray photoelectron spectroscopy (XPS). An electron paramagnetic resonance (EPR) spectrometer is employed to detect the reaction intermediate formed during the polymerization. Results from MALDI-TOF, XPS and EPR reveal that the polymerization of MMA proceeds via a radical mechanism, with the combination of nano-Cu and BPA leading to the formation of BPA residual radicals that initiate the polymerization of MMA.
研究领域:
高分子化学
·
材料科学
·
纳米技术
·
聚合反应工程
·
物理化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 14
·8871-8886
·
IF 4.22
Pi Menghan,Jiang Lichao,Wang Zhisen,Cui Wei,Shi Lingying,Ran Rong
As a new two-dimensional material, MXene has caused extensive research in field of supercapacitor, electromagnetic shielding, strain sensing field due to its excellent mechanical properties and electrical conductivity. MXene with good water dispersibility can be used as an ideal nano-material to enhance the conductivity of hydrogels. However, MXene is easy to accumulate in the hydrogel system, and there are rare articles on MXene hydrogels. In this paper, we combine cellulose nanocrystals (CNCs) and MXene to prepare CNC/MXene nanosheets and take advantage of CNC’s excellent dispersibility in water and the electrostatic repulsion between CNCs to avoid the accumulating of MXene and enhance the mechanical properties of the hydrogel. Based on it, we prepared a physical–chemical double crosslinking and double-network CNC/MXene hydrogel by in situ polymerization and cycle freeze-thaw method. The CNC/MXene hydrogel we prepared exhibited excellent mechanical properties; it could be stretched to 7 times its original length and could reach 1.025 MPa tensile stress. When the compression strain was 80%, the compression stress could reach 1.1 MPa. After unloading the compression pressure, it could rapidly return to its original length within 0.17 s, which would impact greatly on the practical application of CNC/MXene hydrogel. What's more, the conductivity of it was up to 0.4 S/m and the thermal conductivity was 0.38 W/mK. So, the hydrogel prepared by us has a wide range of application prospects in the fields of human motion monitoring, electronic skin, man–machine interface, etc.
研究领域:
材料科学
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传感器技术
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生物医学工程
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DOI
高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 3
·1715-1729
·
IF 5.044
Liu Hongyu,Yang Lin,Zhan Yifei,Lan Jianwu,Shang Jiaojiao,Zhou Mi,Lin Shaojian
In this work, a durable and sunlight-driven self-cleaning superhydrophobic cotton fabric was developed as a multifunctional oil/water separation material. First of all, Ag/AgCl was successfully deposited on the polydopamine modified cotton fabric surface by electrostatic adsorption, then the Ag/AgCl loaded fabric was coated by the polydimethylsiloxane (PDMS) via simple dip-coating method. The as-prepared cotton fabric (PDMS-Ag/AgCl@CF) presented the superhydrophobic property with water contact angle about 155°. Meanwhile, the prepared PDMS-Ag/AgCl@CF exhibited great tolerance and resistance to broad pH and various organic solvents, and it can also withstand 50 cycles of abrasion or 60 min of ultrasound treatment without an apparent decrease of superhydrophobicity, indicating its outstanding mechanical durability. As expected, this durable superhydrophobic fabric can availably separate various oil/water mixtures by gravity-driven force with high separation efficiency (over 97.8%), and it possessed good reusability. Additionally, the PDMS-Ag/AgCl@CF exhibited an outstanding self-cleaning performance under sunlight irradiation, due to visible light photocatalytic performance of Ag/AgCl. Finally, the resultant cotton fabric displayed good antibacterial activity. Therefore, in consideration of its prominent comprehensive performances, it is expected that the PDMS-Ag/AgCl@CF can be used as a promising material for oily water remediation.
研究领域:
材料科学
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环境科学
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光催化
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抗菌材料
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2022
·Vol. 33
·期 10
·1-13
·
IF 4.727
Su Zhixiang,Xing Yuehao,Wang Fei,Xu Zeqin,Gu Yongquan
There are no suitable methods to develop the small-calibre tissue-engineered blood vessels (TEBVs) that can be widely used in the clinic. In this study, we developed a new method that combines electrospinning and in-body tissue architecture(iBTA) to develop small-calibre TEBVs. Electrospinning imparted mechanical properties to the TEBVs, and the iBTA imparted biological properties to the TEBVs. The hybrid fibres of PLCL (poly(L-lactic-co-ε-caprolactone) and PU (Polyurethane) were obtained by electrospinning, and the fibre scaffolds were then implanted subcutaneously in the abdominal area of the rabbit (as an in vivo bioreactor). The biotubes were harvested after four weeks. The mechanical properties of the biotubes were most similar to those of the native rabbit aorta. Biotubes and the PLCL/PU vascular scaffolds were implanted into the rabbit carotid artery. The biotube exhibited a better patency rate and certain remodelling ability in the rabbit model, which indicated the potential use of this hybridization method to develop small-calibre TEBVs.
Sketch map of developing the biotube. The vascular scaffolds were prepared by electrospinning (A). Silicone tube was used as the core, and the vascular scaffold was used as the shell (B). The vascular scaffold and silicone tube were implanted subcutaneously in the abdominal area of the rabbit (C). The biotube was extruded from the silicone tube after 4 weeks ofembedding (D). The biotube was implanted for the rabbit carotid artery (E).
研究领域:
组织工程
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生物材料
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生物医学工程
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 104
·期 5
·2205-2216
·
IF 3.53
Wang Feifei,Wang Yongzheng,Cen Congnan,Fu Linglin,Wang Yanbo
Cyclic diguanylate (c-di-GMP) is a second messenger involved in the regulation of various physiological processes in bacteria. However, its function in spoilage bacteria has not yet been addressed. Here, we studied the function of a tandem GGDEF-EAL domain protein, Sbal_3235, in the spoilage bacterium Shewanella baltica OS155. The deletion of sbal_3235 significantly reduced the c-di-GMP level, biofilm formation, and exopolysaccharide, trimethylamine (TMA), and putrescine production; sbal_3235 deletion also downregulated the expression of the torS and speF genes and affected membrane fatty acid composition. Site-directed mutagenesis in conserved GGDEF and EAL motifs abolished diguanylate cyclase (DGC) and phosphodiesterase (PDE) activity, respectively. These data indicate that Sbal_3235 is an essential contributor to the c-di-GMP pool with bifunctional DGC and PDE activity, which is involved in the biofilm formation and spoilage activity of S. baltica OS155. Our findings expand the biochemical role of c-di-GMP and uncover its link to spoilage activities, providing novel targets for food quality and safety controlling.
研究领域:
微生物学
·
食品科学
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DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2019
·Vol. 149
·期 11
·2973-2983
·
IF 2.372
Luo Wei,Du Hui-Jun,Bonku Emmanuel Mintah,Hou Ya-Li,Li Ling-Ling,Wang Xiao-Qiang,Yang Zhong-Hua
Abstract Enantiopure alcohols have received much attention due to their widespread use as pharmaceutical intermediates. In the asymmetric biosynthesis of enantiopure alcohols, the excellent performance of carbonyl reductase makes it be the best choice as the biocatalysts. In this work, an alkali-tolerant carbonyl reductase ( Bs CR, encoded by yueD ) from Bacillus subtilis (strain 168) was obtained through gene mining, and successfully heterologously expressed in Escherichia coli with pET-32a. Bs CR showed excellent alkali resistance and even can keep more than 70% of its peak activity after incubation in Tris–HCl buffer at pH 9.0 for 40 h. The Michaelis constants and maximal velocity of the Bs CR to NADPH (A) and ethyl 4-chloroacetoacetate (B) are \(K_{m}^{A}\) = 5.390 × 10 −2 mmol/L, \(K_{m}^{B}\) = 1.855 mmol/L, and \(V_{max}\) = 147.3 μmol·min −1 ·mg −1 , respectively. Applying the E. coli BL21(DE3)/pET-32a-yueD to catalyze asymmetric reduction of ethyl 4-chloroacetoacetate and acetophenone, the yield of S -CHBE reached 89.9% and S -1-phenyl ethanol reached 66.7%, and e.e. of both products reached more than 99%. This work provides a novel CR for asymmetric reduction. Graphic Abstract A carbonyl reductase ( Bs CR) and its gene were identified through gene mining, and overexpressed in Escherichia coli BL21(DE3) for whole-cell biocatalytic asymmetric reduction
研究领域:
生物技术
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生物化学
·
药物科学
·
酶学
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DOI
高影响因子
Scientific Reports
·
2022
·Vol. 12
·期 1
·1-13
·
IF 4.996
Ren Zhenkun,Han Xibin,Wang Lixin,Wang Yi
Artesunate was reported to have inhibition effect on tumors via amplified oxidative stress while the lack of intratumoral ferrous ions supply greatly hinders its efficacy. Herein, the AS/GOD@HAZnO NPs we proposed could be efficiently taken in by the affinity between hyaluronic acid and the CD44 receptors. DLS and TEM results manifested the nano-size (~ 160 nm) and circular shape of AS/GOD@HAZnO NPs. Due to the acid-responsive degradation, AS/GOD@HAZnO NPs realized responsive release (up to 80%) in acid environment while only 20% was released in neutral medium. The cellular and in vivo experiment showed that co-delivery of AS and GOD via HAZnO NPs could effectively induce the overproduction of ROS and cut the glucose supply of tumor cells, and thus result in efficient cell apoptosis and tumor inhibition.
研究领域:
纳米技术
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癌症治疗
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药理学
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材料科学
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DOI
PubMed
高影响因子
FISH PHYSIOLOGY AND BIOCHEMISTRY
·
2019
·Vol. 45
·期 2
·539-549
·
IF 1.729
Chen Jiaojiao,Zhang Dianfu,Tan Qingsong,Liu Mengmei,Hu Pengcheng
Dietary arginine (Arg) could improve the intestinal structure and absorption of grass carp ( Ctenopharyngodon idellus ); however, the mechanism of Arg on intestinal morphology improvement was unclear. The present study aimed to explain the possible mechanism of the positive effect of Arg on intestinal epithelial cells of grass carp. An in vitro study was conducted through a primary culture model to assess the growth, cell viability, mRNA expressions of TOR signal pathway, and tight junction proteins of enterocytes after culture in the medium with 6 levels of Arg (0, 0.1, 0.2, 0.5, 1.0, and 2.0 mmol/L). The results showed that 0.5 mmol/L Arg improved the cell number and decreased the lactate dehydrogenase and creatine kinase activities in culture medium ( P < 0.05). The alkaline phosphatase activity in cell lysis buffer was depressed by 1 and 2 mmol/L Arg ( P < 0.05). The nitric oxide (NO) content showed an increasing trend with the Arg content ( P < 0.05), whereas the NO synthase activity showed an opposite trend to NO. TOR expression was higher in 0.2 and 0.5 mmol/L groups, whereas S6K1 expression in 1.0 mmol/L and 2.0 mmol/L groups were lower ( P < 0.05). The mRNA expressions of occludin, claudin 3, and claudin c in 0.5 mmol/L group were the highest, while ZO-1 and claudin b expressions were higher in 0.2 and 0.5 mmol/L groups ( P < 0.05). This study indicated that Arg enhanced the growth and integrity of intestinal epithelial cells of grass carp through upregulation of mRNA expression of TOR signal pathway and tight junction proteins at an optimal Arg content of 0.2–0.5 mmol/L.
研究领域:
水产养殖
·
分子生物学
·
营养学
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DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2022
·Vol. 22
·期 1
·1-12
·
IF 5.26
Yue Runqing,Sun Qi,Ding Jianguo,Li Wenlan,Li Wencai,Zhao Meng,Lu Shouping,Zeng Tingru,Zhang Hua,Zhao Suxian,Tie Shuanggui,Meng Zhaodong
Background Maize rough dwarf disease (MRDD), caused by rice black-streaked dwarf virus (RBSDV) belonging to the Fijivirus genus, seriously threatens maize production worldwide. Three susceptible varieties (Ye478, Zheng 58, and Zhengdan 958) and two resistant varieties (P138 and Chang7–2) were used in our study. Results A set of ATP-binding cassette subfamily B (ABCB) transporter genes were screened to evaluate their possible involvements in RBSDV resistance. In the present study, ZmABCB15 , an ABCB transporter family member, was cloned and functionally identified. Expression analysis showed that ZmABCB15 was significantly induced in the resistant varieties, not in the susceptible varieties, suggesting its involvement in resistance to the RBSDV infection. ZmABCB15 gene encodes a putative polar auxin transporter containing two trans-membrane domains and two P-loop nucleotide-binding domains. Transient expression analysis indicated that ZmABCB15 is a cell membrance localized protein. Over-expression of ZmABCB15 enhanced the resistance by repressing the RBSDV replication ratio. ZmABCB15 might participate in the RBSDV resistance by affecting the homeostasis of active and inactive auxins in RBSDV infected seedlings. Conclusions Polar auxin transport might participate in the RBSDV resistance by affecting the distribution of endogenous auxin among tissues. Our data showed the involvement of polar auxin transport in RBSDV resistance and provided novel mechanism underlying the auxin-mediated disease control technology.
研究领域:
植物病理学
·
分子生物学
·
功能基因组学
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DOI
PubMed
高影响因子
BIOLOGY AND FERTILITY OF SOILS
·
2021
·Vol. 57
·期 3
·399-407
·
IF 6.432
Li Yaying,Zhang Yang,Chapman Stephen James,Yao Huaiying
Sorghum has a great capacity to release biological nitrification inhibitors (BNIs), but the inhibitory effect on nitrification and ammonia oxidizer populations under planted soil conditions is unclear. A pot experiment with three nitrogen (N) application rates (0, 50, and 200 mg N kg −1 ) was set up to detect the influence of sorghum growth on soil nitrification and investigate the function of blocking the activity of ammonia oxidizers. A 15 N-labeled experiment was also conducted to detect the N form absorbed by sorghum. Sorghum root exudates were collected at 30 days after transplanting to hydroponic culture and added into cultured soil to determine the shifts in the populations of nitrifiers. The 15 N labeling experiment showed that the uptake rate by sorghum of ammonium N fertilizer was 24% and that of nitrate N fertilizer was 9%, indicating that sorghum was an ammonium using plant. Compared with unplanted soil, sorghum planting had a significant inhibitory effect on the nitrification process even at the high-N fertilizer rates. Autotrophic nitrification was the prevailing process, and sorghum root exudation inhibited this process as much as dicyandiamide (DCD, 10 mg kg −1 ). Root exudates had a significant inhibitory effect on ammonia-oxidizing bacteria (AOB) but had no effect on ammonia-oxidizing archaea (AOA).
研究领域:
土壤微生物学
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植物生理学
·
环境科学
·
农学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-11
·
IF 6.408
Wang Lin,Wang Beibei,Kang Kai,Ji Xueping,Wang Bing,Li Congwei,Ren Jujie
A new strategy is proposed for ultrasensitive detection of chlorogenic acid (CGA) by fabricating an electrochemiluminescence resonance energy transfer (ECL-RET) sensing platform. The novel system designed by introducing ruthenium-based 2D metal–organic framework nanosheets (Ru@Zn-MOF) as ECL acceptor and L-cysteine capped CdS quantum dots (L-CdS QDs) as ECL donor, exhibited good ECL response. The possible mechanism of the modified electrode surface reaction was discussed. Modifying of the electrode surface by application of L-CdS QDs directly on ultrathin MOF nanosheets greatly shortened the electron-transfer distance and reduce energy loss, therefore significantly improving the ECL efficiency. The prepared sensor demonstrated good stability and highly selective detection of the target molecule. Under optimal conditions, the constructed sensor for the detection of CGA exhibited a wide linear range from 1.0 × 10 −10 to 1.0 × 10 −4 mol·L −1 and a low detection limit of 3.2 × 10 −11 mol·L −1 with a correction coefficient of 0.995. The recovery for spiked samples was calculated to be 94.4–109% and the RSD was 1.07–1.72% in real samples. The obtained sensor is considered to be a promising platform for CGA detection. Graphical abstract Electrochemiluminescence resonance energy transfer (ECL-RET) sensing platform is used for the detection for chlorogenic acid.
研究领域:
分析化学
·
材料科学
·
生物医学检测
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DOI
高影响因子
CELLULOSE
·
2020
·Vol. 28
·期 2
·691-701
·
IF 4.21
Wang Ruochun,Rong Liduo,Ni Shujing,Wang Qiankun,Wang Bijia,Mao Zhiping,Feng Xueling,Yuan Jinying,Sui Xiaofeng
A novel approach was developed to prepare cellulose acetoacetate- graft -copolymers via horseradish peroxidase (HRP)-mediated polymerization. Cellulose acetoacetate (CAA), the macroinitiator, was obtained by transesterification between tert -butyl acetoacetate ( t -BAA) and cellulose in ionic liquid. The CAA- graft -polyacrylamide (CAA- g -PAM) was synthesized using a ternary initiator system consisting of CAA, HRP and hydrogen peroxide (H 2 O 2 ). The results of surface-initiated polymerization showed that the formation of free polymers could be inhibited in this process. The kinetics exhibited that this method is a powerful tool for obtaining graft copolymers. The effects of H 2 O 2 concentration and reaction temperature were investigated to explore the optimal conditions for the process of enzymatic initiation. The synthesized copolymers were characterized by FT IR, NMR, TGA and elemental analysis to confirm the successful grafting of polyacrylamide chains onto the cellulose backbones. Furthermore, generality of the HRP-mediated graft polymerization approach was demonstrated using various monomers, including 2-hydroxyethyl methacrylate (HEMA), methyl methacrylate (MMA) and sulfobetaine methacrylate (SBMA). This efficient and robust strategy has great potential in fabrication of cellulose-based functional polymers. Graphic abstract Enzymatic grafting polymerization from cellulose acetoacetate
研究领域:
材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）