ANALYTICA CHIMICA ACTA
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2017
·
IF 4.95
Yashuang Hei,Xiqian Li,Xiao Zhou,Jingju Liu,Mimi Sun,Tianze Sha,Cuixing Xu,Wanyi Xue,Xiangjie Bo,Ming Zhou
In this paper, kelp ( Laminaria japonica ), as a kind of abundant biomass, is used as the precursor for the preparation of kelp-derived hierarchical meso-macroporous carbons (K-dHMMCs) through the carbonization under nitrogen (N 2 ) atmosphere at high temperature. The K-dHMMCs exhibits the unique structure with high specific surface area of 416.02 m 2 g −1 , large pore volume of 0.24 cm 3 g −1 , the hierarchical meso-macroporous size distribution centered at 2, 12 and 82 nm and high density of defective sites, enabling K-dHMMCs attractive for the electrocatalysis . Drop-casting K-dHMMCs on the glassy carbon (GC) surface allows the construction of K-dHMMCs based electrochemical sensing platform, which shows electrocatalytic activities towards many electroactive molecules, such as potassium ferricyanide , nicotinamide adenine dinucleotide (NADH), hydrogen peroxide (H 2 O 2 ), dopamine (DA), uric acid (UA), ascorbic acid (AA), epinephrine (EP), l -tyrosine (Tyr) and acetaminophen (APAP). Especially, the K-dHMMCs modified GC (K-dHMMCs/GC) electrode exhibits higher sensitivity, wider linear range, and lower detection limit than both carbon nanotubes modified GC (CNTs/GC) and GC electrodes for H 2 O 2 detection, which makes the K-dHMMCs/GC electrode to be able to determine the H 2 O 2 levels in human urine sample and monitor the H 2 O 2 released from human cancer cells. These results demonstrate that K-dHMMCs/GC possesses a great potential for conventional electrochemical sensing applications.
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高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
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2017
·Vol. 23
·期 64
·16179-16183
·
IF 5.317
Zao Wang,Fengyu Xie,Zhiang Liu,Gu Du,Abdullah M. Asiri,Xuping Sun
Graphical A nickel borate nanoarray on carbon cloth (Ni−Bi/CC) behaves as an efficient catalyst electrode for H 2 O 2 electroreduction in neutral media. As a non-enzymatic electrochemical H 2 O 2 sensor, such Ni−Bi/CC shows superior sensing performances with a fast response time (less than 3 s), a low detection limit (0.85 n m , S / N =3), and a high sensitivity (18320 μA m m cm −2 ). It is highly attractive to construct natural enzyme-free nanoarray architecture as a 3D catalyst for hydrogen peroxide detection due to its great specific surface area and easy accessibility to target molecules. In this communication, we demonstrate that nickel borate nanoarray supported on carbon cloth (Ni−Bi/CC) behaves as an efficient catalyst electrode for H 2 O 2 electro-reduction in neutral media. As a non-enzymatic electrochemical H 2 O 2 sensor, such Ni−Bi/CC shows superior sensing performances with a fast response time (less than 3 s), a low detection limit (0.85 n m , S/ N =3), and a high sensitivity (18320 μA m m cm −2 ). Importantly, it also demonstrates favourable reproducibility and long-term stability.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2017
·
IF 3.012
Yangping Wen,Juan Chang,Lanjiao Xu,Xiaoning Liao,Ling Bai,Yingdong Lan,Mingfang Li
Abnormal level of purines , especially uric acid (UA), xanthine (XA) and hypoxanthine (HX) in organisms is an important marker for early diagnosis of animal (human) disease. In this paper, an simultaneous analysis of UA, XA and HX in biological samples was successfully realized using the fabricated voltammetric sensor based on the nanocomposite of both nano-rod clay mineral palygorskite (Pal) and two-dimensional nano-layered nitrogen doped graphene (NG). A water-soluble carboxymethyl cellulose (CMC) salt was simultaneously decorated both Pal and NG to improve the water dispersibility, and their nanocomposite was characterized. Under optimized conditions, The as-prepared nanocomposite electrode (CMC-Pal-NG/GCE) could realize their simultaneous voltammetric analysis in linear ranges from 0.5 to 60 μM for UA, 0.2 to 20 μM for XA, and 0.6 to 55 μM for HX. The limit of detection of three purines (UA,XA and HX) is calculated to be 0.1 for UA, 0.05 for XA and 0.4 μM for HX, and their possible detailed mechanisms for electro-catalytic oxidation and chemical reaction was discussed, which was successfully empolyed for simultaneous voltammetric analysis of three purines in real samples with good reproducibility, repeatability and stability. Satisfactory results indicated that the obtained nanocomposite will provide a promising candidate for the simultaneous electrochemical determination of UA, XA and HX in analysis of practical samples.
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JOURNAL OF FLUORESCENCE
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2017
·Vol. 28
·期 1
·269-276
·
IF 1.461
Zhao Chunxi,Jiao Yang,Hua Jianhao,Yang Jian,Yang Yaling
Nitrogen-doped carbon quantum dots (N-CQDs) were synthesized though a facile, economical and straightforward hydrothermal method by using polyacrylamide as both carbon and nitrogen sources. The as-prepared N-CQDs offered high quantum yield of 23.1%, exhibited good water solubility and fluorescence properties. Moreover, the N-CQDs can be used as effective probes for sensitive and selective detection of dopamine. Fluorescence of N-CQDs was effectively quenched after the addition of dopamine owing to dopamine would be transformed into dopamine-quinone under alkaline conditions. A good linear relationship between fluorescence quenching and the concentration of dopamine in the range 0.1–200 μM was obtained with a low detection limit of 0.05 μM. The proposed method showed high selectivity for dopamine in the presence of potential interfering species. Moreover, this method was successfully applied to the determination of DA in urine sample with satisfactory recoveries.
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TALANTA
·
2017
·
IF 4.162
Li-Ping Jia,Li-Juan Wang,Rong-Na Ma,Lei Shang,Wei Zhang,Qing-Wang Xue,Huai-Sheng Wang
In the present work a highly sensitive and selective aptasensor was developed for the determination of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) based on the hybridization chain reaction (HCR) signal amplification. It was observed that the aptamer of 8-OH-dG could hybridize with the capture DNA immobilized on the gold electrode with a sticky tail left, which initiated the HCR and led to the formation of extended dsDNA structure on the electrode surface. Then the electroactive species ([Ru(NH 3 ) 6 ] 3+ , RuHex) intercalated into the dsDNA grooves to generate the amplified signal. However, in the presence of 8-OH-dG, the aptamer containing G-rich nucleic acid sequences would be induced to form a G-quadruplex structure, which made it impossible to continue the HCR. So the detection signal will significantly decrease. Under the optimal conditions, the peak current of RuHex was linear with the logarithm of 8-OH-dG concentration in the range from 10 pM to 100 μM with the detection limit of 2.5 pM. By integrating the merits of enzyme-free amplification power of the HCR and the inherent high sensitivity of the electrochemical technique, the prepared aptasensor not only showed high sensitivity for the detection of 8-OH-dG, but also exhibited good selectivity against to the uric acid , an important interferent in the urine sample. Particularly, the aptasensor was applied to detect 8-OH-dG in urine samples with satisfactory results.
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JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 21
·4160-4167
·
IF 2.557
Jing Wang,Dongfang Shi,Meizhu Zheng,Bing Ma,Jing Cui,Chunming Liu,Chengyu Liu
Natural products have become one of the most important resources for discovering novel xanthine oxidase inhibitors, which are commonly employed in the treatment of hyperuricemia and gout. However, to date, few reports exist regarding the use of monoterpene glycosides as xanthine oxidase inhibitors. Thus, we herein report the use of ultrafiltration coupled with liquid chromatography in the screening of monoterpene glycoside xanthine oxidase inhibitors from the extract of Paeonia lactiflora ( P. lactiflora ), and both high-performance counter-current chromatography and medium-pressure liquid chromatography were employed to separate the main constituents. Furthermore, the xanthine oxidase inhibitory activities and the mechanisms of inhibition of the isolated compounds were evaluated using a multi-mode microplate reader by Molecular Devices. As a result, three monoterpene glycosides were separated by combined high-performance counter-current chromatography and medium-pressure liquid chromatography in purities of 90.4, 98.0, and 86.3%, as determined by liquid chromatography. These three compounds were identified as albiflorin, paeoniflorin, and 1- O -β-ᴅ-glucopyranosyl-8- O -benzoylpaeonisuffrone by electrospray ionization tandem mass spectrometry, and albiflorin and paeoniflorin were screened as potential xanthine oxidase inhibitors by ultrafiltration with liquid chromatography. The evaluation results of xanthine oxidase inhibitory activity corresponded with the screening results, as only albiflorin and paeoniflorin exhibited xanthine oxidase inhibitory activity.
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Nanoscale
·
2017
·Vol. 9
·期 44
·17561-17570
·
IF 7.367
Xiao Liu,Wei Qi,Yuefei Wang,Rongxin Su,Zhimin He
Metal–organic frameworks (MOFs) have drawn extensive research interest as candidates for enzyme immobilization owing to their tunable porosity, high surface area, and excellent chemical/thermal stability. Herein, we report a facile and universal strategy for enzyme immobilization using highly stable hierarchically porous metal–organic frameworks (HP-MOFs). The HP-MOFs were stable over a wide pH range (pH = 2–11 for HP-DUT-5) and met the catalysis conditions of most enzymes. The as-prepared hierarchical micro/mesoporous MOFs with mesoporous defects showed a superior adsorption capacity towards enzymes. The maximum adsorption capacity of HP-DUT-5 for glucose oxidase (GOx) and uricase was 208 mg g−1 and 225 mg g−1, respectively. Furthermore, we constructed two multi-enzyme biosensors for glucose and uric acid (UA) by immobilizing GOx and uricase with horseradish peroxidase (HRP) on HP-DUT-5, respectively. These sensors were efficiently applied in the colorimetric detection of glucose and UA and showed good sensitivity, selectivity, and recyclability.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Longhua Ding,Hongmei Yang,Shenguang Ge,Jinghua Yu
A simple and effective fluorescent assay for the determination of vitamin B 12 was developed. In this study, carbon dots (CDs) were prepared by one-pot hydrothermal method and directly used as a fluorophore in the inner filter effect (IFE). Both of the maximum absorption peak of vitamin B 12 and excitation maxima of CDs are located at 360 nm, hence, the excited light of CDs can be absorbed by vitamin B 12 , resulting in the fluorescence reduction of CDs. And the fluorescence intensity of CDs decreases with the increasing concentration of vitamin B 12 . This IFE-based sensing strategy shows a good linear relationship between the normalized fluorescence intensity and the concentration of vitamin B 12 ranging from 0 to 60 μM, with a limit of detection (LOD) of 0.1 μM at a signal-to-noise ratio of 3. Furthermore, this proposed approach was successfully applied to vitamin B 12 sensing in injections. This IFE sensing platform based on various fluorescent nanomaterials has a high promise for the detection of other biomolecules due to its inherent convenience.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Lijun Zhang,Guiheng Wang,Di Wu,Can Xiong,Lei Zheng,Yunsheng Ding,Hongbo Lu,Guobing Zhang,Longzhen Qiu
In this study, an organic electrochemical transistor sensor (OECT) with a molecularly imprinted polymer (MIP)-modified gate electrode was prepared for the detection of ascorbic acid (AA). The combination of the amplification function of an OECT and the selective specificity of MIPs afforded a highly sensitive, selective OECT sensor. Cyclic voltammetry and electrochemical impedance spectroscopy measurements were carried out to monitor the stepwise fabrication of the modified electrodes and the adsorption capacity of the MIP/Au electrodes. Atomic force microscopy was employed for examining the surface morphology of the electrodes. Important detection parameters, pH and detection temperature were optimized. With the change in the relative concentration of AA from 1 μM to 100 μM, the MIP-OECT sensor exhibited a low detection limit of 10 nM (S/N > 3) and a sensitivity of 75.3 μA channel current change per decade under optimal conditions. In addition, the MIP-OECT sensor exhibited excellent specific recognition ability to AA, which prevented the interference from other structurally similar compounds (e.g., aspartic acid , glucose, uric acid , glycine, glutathione , H 2 O 2 ), and common metal ions (K + , Na + , Ca 2+ , Mg 2+ , and Fe 2+ ). In addition, a series of vitamin C beverages were analyzed to demonstrate the feasibility of the MIP-OECT sensor. Using the proposed principle, several other sensors with improved performance can be constructed via the modification of organic electrochemical transistors with appropriate MIP films.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2017
·
IF 4.233
Juan Zhang,Liao Xu,Bo Zhou,Yinyan Zhu,Xiaoqing Jiang
The pristine graphene can be easily prepared in isopropanol-water mixture with salts as assistant via liquid-phase exfoliation method. The concentration of graphene dispersion reaches as high as 0.565 mg/mL. The graphene film prepared by drop-casting method shows an excellent electrical conductivity (7.095 × 10 4 S/m). Furthermore, an electrochemical biosensor based on the pristine graphene shows high selectivity and sensitivity for the determination of dopamine. The linear detection range for dopamine is 2.5–1500 μM with detection limit of 1.5 μM. This method provides a potential process for preparing high-quality graphene ready-to-use in low-boiling point solvent.
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TALANTA
·
2017
·
IF 4.162
Qi Wang,Qian Zhou,Qicheng Zhang,Rongjia Shi,Shangshang Ma,Wenbo Zhao,Min Zhou
Preparation of high performance electrochemical biosensing interface for the sensitive and rapid detection of human metabolites is of great interest for health care and biomedical science. In this paper, based on the adhesion technique of marine mussels, we designed and prepared a novel biosensor with a micro/nano-biointerface of Fe 3 O 4 -Mn 3 (PO 3 ) 2 @Ni foam, which offered a three dimensional (3D) living environment for real cell. The constructed biosensor with a 3D micro/nano-biointerface of Fe 3 O 4 -Mn 3 (PO 3 ) 2 @Ni foam was characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and elemental mapping. Furthermore, the electrochemical experiments by electrochemical method for detection of superoxide anion (O 2 •− ) in situ released by cells were carried out by this biosensor we proposed. Results indicated that the 3D interface of mussel-inspired Fe 3 O 4 -Mn 3 (PO 3 ) 2 @Ni foam offered an amicable platform for promoting cell adhesion, which was beneficial for enhancing biosensing activity. This proposed sensing platform provided high electroactivity and excellent electron transport with a lower detection limit (0.0170 μM), wider linear range 0.04–2.44 μM) and short diffusion distance to reaction sites. The case achieved the accurate detection of O 2 •− (in situ released by cells) based on the combination of mussel-inspired biomimetic adhesion technique, 3D micro/nano-biointerface construction and electrochemical biosensing technique.
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EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Jingfang Li,Jiaqi Liu,Jiajun Wang,Wen Li,Fang Wang
Wearable electrochemical sensors for glucose analysis hold significant potential for non-invasive monitoring of sweat biomarkers. However, the complex and expensive preparation process has limited the application of these sensors. In this study, we proposed an electrochemical glucose biosensor based on poly (2-hydroxyethyl methacrylate) (PHEMA) hydrogel initiated via iron ion and phenol hydroxyl groups. Copper nanoparticles are then incorporated into the hydrogel, enhancing the conductivity and electrocatalytic performance of the hydrogel electrode. The composite hydrogel, characterized by its rich metal coordination and hydrogen bonds, demonstrated remarkable cyclic adhesion properties, stretchability, and self-healing capability. Subsequently, this hydrogel is modified onto a screen-printed electrode (SPE) to create an in vitro skin patch for glucose detection in human sweat. The biosensor exhibits a wide linear range for glucose concentration of 0–0.2 mM and 0.2–4 mM, with low detection limit of 9.99 μM. The sensor exhibits good stability, selectivity and flexibility, and demonstrates a strong correlation with glucose measurements obtained from real sweat using a glucose assay kit. This sweat sensor holds great development potential, providing broad prospects for future non-invasive detection applications.
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