ANALYTICAL LETTERS
·
2020
·
IF 1.467
Yuying Song,Yuanyi Zhang,Yiqun Huang,Yuxia Fan,Keqiang Lai
With the large-scale use of pesticides, food safety issues caused by their residues have been drawn increasing concern. In this study, surface-enhanced Raman spectroscopy (SERS) coupled with a Au@Ag nanoparticle (NP)-graphene oxide (GO) composite substrate which was synthesized by a simple self-assembly method was applied for the rapid determination of thiram residues in fruit juice. To obtain high SERS activity, the loading and the aggregation of Au@Ag NP on the graphene oxide sheets were adjusted by controlling the size of Au@Ag nanoparticle and the concentration of graphene oxide in solution. Using the optimal conditions, the limit of detection for thiram standard solution was 6.3 ng/mL, while the limits of detection for thiram in apple juice and grape juice without any pretreatment were 36.7 and 134.4 ng/mL, respectively. The entire analytical process required less than 20 min. The results indicated that SERS technology coupled with Au@Ag nanoparticle graphene oxide composite substrate was a simple, rapid, and sensitive approach for the determination of pesticide residues in fruit juice.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 48
·30102-30108
·
IF 3.361
Setareh Rostami-Javanroudi,Masoud Moradi,Kiomars Sharafi,Nazir Fattahi
In the present research, several novel and natural hydrophobic deep eutectic solvents (DESs) were prepared using methyltrioctylammonium chloride (MTOAC) as the hydrogen bond acceptor (HBA) and different types of straight chain alcohols as hydrogen bond donors (HBDs). One of the DESs composed of MTOAC and n-butanol was advantageously used to develop a vortex-assisted liquid phase microextraction (VALPME) method combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) for the determination of common acaricides in fruit juice samples. Several important parameters influencing extraction efficiency were investigated and optimized, including the type and volume of DES, sample solution pH, effect of salt addition and, extraction and vortex time. Under optimal experimental conditions, the method showed good linearity with the correlation coefficients (R2) of 0.9986–0.9991 in the linear range of 2–300 μg L−1, low limits of detection of 0.5–1 μg L−1 and acceptable extraction recoveries in the range of 85–93%. The proposed method was successfully applied for the extraction and preconcentration of trace acaricides in real fruit juice samples, and the results demonstrated the potential of the synthesized DESs for the extraction and determination of contaminants in aqueous samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2021
·Vol. 114
·期 6
·2430-2438
·
IF 2.381
Su Sha,Jian Chengzhi,Zhang Xiaohe,Fang Sensen,Peng Xiong,Piñero Jaime C,Chen Maohua
Grapholita molesta is one of the most important fruit pests worldwide. Abamectin is a biological pesticide frequently used to control fruit borers like G. molesta in part owing to its translaminar properties. In this study, we characterized the toxicity of abamectin to G. molesta larvae using the diet incorporation method. The sublethal effects of abamectin on the development, reproduction, detoxification enzyme activity, and related gene expression of G. molesta were assessed. The results showed that the LC20 and LC50 values of the insecticide against G. molesta 72 h post-treatment were 1.17 mg L–1 and 5.85 mg L–1, whereas the LC20 and LC50 values 96 h post-treatment were 0.34 mg L–1 and 3.63 mg L–1. When compared to the control, sublethal concentrations of abamectin 1) significantly increased the mortality of the larvae, prepupae, and pupae of G. molesta, 2) prolonged the duration of 3rd to 5th instar larva, prepupal and pupal periods, 3) shortened the longevity of adults, and 4) reduced female fecundity. The enzymatic activity of glutathione S-transferase (GST) varied significantly after exposure to sublethal concentrations of abamectin, but the cytochrome P450 monooxygenases and carboxylesterase activity were not significantly affected. Thirteen of the 25 GST genes were significantly upregulated under different sublethal concentrations of abamectin. The combined findings increase our understanding of the effects of abamectin on G. molesta and the potential role of GSTs in the metabolic interactions of abamectin in this pest, and have applications for more rational and effective use of abamectin to control G. molesta.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Liyang Du,Xuedong Wang,Tingting Liu,Jieyi Li,Junxia Wang,Ming Gao,Huili Wang
Herein, we developed a novel three-layer nanocomposite for adsorption/extraction of organophosphorus pesticides in fruit juice samples. This magnetic nanocomposite was fabricated by coating polydopamine and Mg/Al layered double hydroxides onto NiFe 2 O 4 nanoparticles , which overcomes the shortcomings of low surface area and easy agglomeration associated with conventional Fe-based crystal nanoparticles. Additionally, the higher dispersibility of polydopamine and larger specific surface area of Mg/Al layered double hydroxides are conducive to rapid dispersion and efficient adsorption of organophosphorus pesticides by the synthesized nanocomposites, thereby greatly improving the efficiency of magnetic solid-phase extraction. The extraction method based on as-synthesized nanocomposites provided average extraction recoveries of 84.9% for methamidophos , parathion and phoxim , an increase of ca. 30% and 19% over those of NiFe 2 O 4 and NiFe 2 O 4 @polydopamine, respectively. The main operational parameters were firstly screened and then optimized using Plackett-Burman and central-composite design, respectively. Under optimized conditions, this proposed method yielded low detection limits (0.06–0.13 μg L − 1 ) and high intra- and inter-day precisions (2.7–4.5%). Average extraction recoveries for organophosphorus pesticides were 81.8–94.4% in grape, apple, orange, peach and mango fruit juices. To sum up, it integrates highly efficient preconcentration/extraction/dispersion, as well as rapid separation/collection into one synchronous step, satisfies the technical requirement of modern analytical pretreatment and thus enriches the multi-functional application of Fe-based nanoparticles.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Dandan Li,Man He,Beibei Chen,Bin Hu
In this work, magnetic nanoporous carbons (MNPCs) based on Zn/Co-MOFs was synthesized via a facial one-step carbonization method. The prepared MNPCs exhibited high specific surface areas, good chemical stability and high extraction efficiency for interest organophosphorus pesticides (OPPs). The good extraction performance of the MNPCs for interest OPPs can be primarily attributed to π-π interaction and hydrophobic interaction between MNPCs and OPPs. Based on it, MNPCs-based MSPE-GC-FPD method was established for the quantification of five OPPs in fruit samples. The main experimental conditions for MSPE of five target OPPs were investigated systematically and optimized, such as the desorption solvent and volume, extracted volume, ionic strength, extraction time and desorption time. Under the optimal conditions, the limits of detection (LODs, S/N = 3) were found to be in the range of 0.018 (ethion) -0.045 (malathion) μg/L. The linearity was 0.05-100 μg/L for phorate, diazinon, ethion and 0.1-100 μg/L for malathion, fenthion respectively. The reproducibility of the method was in the range of 3.5-9.7%. The recovery experiments were carried out by spiking target OPPs at three concentration levels to validate the accuracy of the proposed method, and the recoveries for target OPPs were 84-116%. The results show that the proposed method in this work can be successfully used to analyze OPPs residue in fruit sample.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 15
·5659-5665
·
IF 1.938
Lin Hao,Chenhuan Wang,Xiaoxing Ma,Qiuhua Wu,Chun Wang,Zhi Wang
In this paper, a three-dimensional graphene-based magnetic nanocomposite (3D-G-Fe3O4) was synthesized and used as an effective adsorbent for the extraction of four phthalate esters (dimethyl(o-)phthalate, diethyl(o-)phthalate, diallyo(o-)phthalate and bis(2-propylheptyl)phthalate) from fruit juice prior to high performance liquid chromatography analysis. The three-dimensional nanoporous structure of the 3D-G endows the new material with a high adsorption capacity. The properties and morphology of the 3D-G-Fe3O4 were characterized by transmission electron microscopy, scanning electron microscopy and infrared spectroscopy. Several experimental parameters affecting the extraction efficiencies, such as the amount of the 3D-G-Fe3O4, extraction time, sample pH, salt addition and desorption conditions were optimized. Under the optimum conditions, the limits of detection (S/N = 3) of the method for the analytes were between 0.04 and 0.13 ng mL−1. The recoveries of the method for the analytes were in the range from 87.0% to 97.8%. The result showed that the 3D-G-Fe3O4 has an excellent adsorption capacity for the analytes.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Fengli Chen,Jia Jia,Qiang Zhang,Huiyan Gu,Lei Yang
In this work, a modified technique was developed to separate essential oil from the fruit of Amorpha fruticosa using microwave-assisted hydrodistillation concatenated liquid-liquid extraction (MHD-LLE). The new apparatus consists of two series-wound separation columns for separating essential oil, one is the conventional oil-water separation column, and the other is the extraction column of components from hydrosol using an organic solvent. Therefore, the apparatus can simultaneously collect the essential oil separated on the top of hydrosol and the components extracted from hydrosol using an organic solvent. Based on the yield of essential oil in the first and second separation columns, the effects of parameters were investigated by single factor experiments and Box–Behnken design. Under the optimum conditions (2 mL ethyl ether as the extraction solvent in the second separation column, 12 mL/g liquid-solid ratio, 4.0 min homogenate time, 35 min microwave irradiation time and 540 W microwave irradiation power), satisfactory yields for the essential oil in the first separation column (10.31 ± 0.33 g/kg) and second separation column (0.82 ± 0.03 g/kg) were obtained. Compared with traditional methods, the developed method gave a higher yield of essential oil in a shorter time. In addition, GC–MS analysis of the essential oil indicated significant differences of the relative contents of individual volatile components in the essential oils obtained in the two separation columns. Therefore, the MHD-LLE technique developed here is a good alternative for the isolation of essential oil from A. fruticosa fruit as well as other herbs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2017
·
IF 3.144
Guan-Lin Chen,Xin Zhang,Song-Gen Chen,Men-Di Han,Yong-Qing Gao
In order to explore the potential of different subtropical fruit leaves as natural sources of bioactive compounds, free, esterified and insoluble-bound forms of total phenolic contents (TPC) and total flavonoid contents (TFC) were determined. The antioxidant activities of fruit leaves was determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC) assays. Furthermore, several phenolic compounds were detected using ultra performance liquid chromatography (UPLC) technique. Rutin, (+)-catechin, isoquercitrin, quercitrin and quercetin were widely found in these fruit leaves. The highest TPC, TFC and antioxidant capacities were found in Averrhoa carambola , Dimocarpus longgana Lour, Litchi chinensis Sonn , Mangifera indica and Psidium guajava Linn . The results implied that these leaves are important natural sources of antioxidants for preparing functional food ingredients and preventing oxidative stress diseases.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Fungi
·
2024
·Vol. 10
·期 10
·726
·
IF 4.2
Di Wang,Haijue Zhang,Lingkui Meng,Xinyu Tan,Rong Liu,Qingchao Gao,Yan Wu,Yuhan Zhu,Xueyan Ren,Yongcai Li,Qingjun Kong
Black spot caused byAlternaria alternatais one of the most common postharvest diseases in fruit and vegetables. A comprehensive investigation into its pathogenicity mechanism is imperative in order to propose a targeted and effective control strategy. The effect of nitric oxide (NO) on the pathogenicity ofA. alternataand its underlying mechanism was studied. The results showed that treatment with 0.5 mM L−1of sodium nitroprusside (SNP) (NO donor) increased the lesion diameter ofA. alternatain vivo and in vitro, which was 22.8% and 13.2% higher than that of the control, respectively. Exogenous NO treatment also induced endogenous NO accumulation by activating nitric oxide synthase (NOS). In addition, NO triggered an increase in reactive oxygen species (ROS) levels. NO enhanced activities and gene expression levels of NADPH oxidase (NOX), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), and glutathione reductase (GR). Moreover, NO stimulated cell wall degrading enzymes by activating the corresponding gene expression in vivo and in vitro. These results suggested that exogenous NO promoted the pathogenicity ofA. alternataby inducing ROS accumulation and activating antioxidants and cell wall degrading enzymes. The present results could establish a theoretical foundation for the targeted control of the black spot disease in pear fruit.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Keyan Liu,Wei Feng,Yanru Li,Donglai Han,Tianna Wu,Kaiyue Li,Shuo Yang
Surface-enhanced Raman spectroscopy (SERS) with enormous advantages has emerged as a powerful tool for both highly sensitive structural detection of analytes and various imaging applications. A notable advancement in the realm of multifunctional SERS substrates is the integration of magnetic nanoparticles endowed with magnetic manipulation capabilities and noble metal nanoparticles exhibiting plasmonic resonance properties. This synergistic combination offers substantial practical benefits for trace analysis across diverse fields. In this paper, a new paradigm of Fe 3 O 4 @PEI-DTC-Ag (Fe 3 O 4 @PD-Ag) photomagnetic nanocomposites based on magnetic Fe 3 O 4 nanoparticles (NPs), the noble metal silver quantum dots (Ag QDs) and the adhesive layer polyethyleneimine dithiocarbamate (PEI-DTC) for convenient recycling and ultrasensitive SERS detection was developed by engineering hydrothermal and seed-growth methods. The SERS performance of the Fe 3 O 4 @PD-Ag substrates with varying amounts of Ag was evaluated by detecting 4-aminothiophenol (4-ATP). Among the tested samples, Fe 3 O 4 @PD-Ag-4 with a silver solution volume of 200 ml exhibited the optimal performance, showcasing an enhancement factor ( EF ) of 4.418 × 10 5 . Moreover, the relative standard deviations (RSDs) were calculated to be less than 11.5 %, demonstrating the SERS substrates exhibit good uniformity and spectral reproducibility. Of note, the SERS substrate has demonstrated successful trace detection of thiram molecules, achieving a limit of detection (LOD) of up to 10 -9 M. The intensity of SERS signals shows a linear correlation with the logarithm of thiram concentration, ranging from 10 -4 to 10 -9 M, thus providing compelling evidence for quantitative detection. Importantly, the Fe 3 O 4 @PD-Ag-4 SERS substrate also showcases outstanding performance for detecting thiram in real food, underscoring its significant potential for application in food safety monitoring.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Shikai Zhang,Houshen Li,Bowen Zhang,Shiyun Ai,Yang Shan,Shenghua Ding
Converting fruit peel wastes into degradable bioplastics has brought dawn to solve the severe plastic pollution and inefficient organic food waste management. However, it remains a challenge to convert peel wastes into bioplastics without decomposing their biocomponents. Furthermore, most of the bioplastics currently being researched have poor performance, especially in terms of trade-offing high strength, toughness, and good processability. Here, we utilize a noncovalent-mediated design to convert peel waste into pectocellulosic bioplastic that features the in situ ordered self-assembly of cellulose and pectin in the peel into a structure similar to a carboxylic acid dimer via hydrogen bonding. Benefiting from efficient energy dissipation mechanisms and reversible hydrogen bonding interactions, pectocellulosic bioplastics exhibit excellent mechanical properties (superior to most petrochemical-based and bio-based plastic materials), and can be molded into 2D/3D shapes or reprocessed into new plastics, realizing a combination of high strength (up to 50.5 MPa), toughness (up to 5.1 MJ/m 3 ) and good processability. These bioplastics also combine biosafety (48 h cell viability > 85 %), biodegradability, and durability. Based on these characteristics, these pectocellulosic bioplastics can be considered as a candidate for petrochemical plastics, particularly suitable for some sensitive applications such as food inner packaging. Excitingly, the universality of this strategy in a variety of peel wastes has been verified. This work highlights the transformative potential of converting peel waste into valuable materials, providing a sustainable solution to plastic pollution and organic waste management.
✓ 已验证
产品关联
DOI
高影响因子
ELECTROPHORESIS
·
2012
·Vol. 33
·期 15
·2454-2463
·
IF 3.303
Yingying Wen,Lingxin Chen,Jinhua Li,Yanling Ma,Shoufang Xu,Zhong Zhang,Zongliang Niu,Jaebum Choo
A simple and sensitive method for the simultaneous determination of four triazines from soil, strawberry, and tomato samples was developed by selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) coupled to micellar electrokinetic chromatography (MEKC). Using atrazine as template, the synthesized molecularly imprinted polymers (MIPs) were employed as the dispersion sorbent of MSPD to successfully extract atrazine and its analogs of simazine, ametryn, and propazine from the three different real samples, while matrix interferences were effectively eliminated simultaneously under the optimum extraction conditions. Excellent separation was achieved within 7 min by using an optimized buffer system composed of 30 mmol/L ammonium acetate, 20 mmol/L SDS, and 15% ACN at pH 9.45, obtained by orthogonal design. Good linearity was obtained in a range of 0.5–25 μg/g with the correlation coefficients R 2 ≥0.9991 except for strawberry sample within 1–25 μg/g, and limits of detection were between 12.9–31.5 ng/g in all the three samples. The average recoveries of the four triazines at three different spiked levels were ranged from 53.5 to 98.4% with the relative standard deviations of 1.28–4.89%. This method was proved convenient, costeffective, and environmental benign and could be used as an alternative tool to the existing methods for analyzing the residues of triazines in soil, fruit, and vegetable samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）