APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
Zhao Shanshan,Du Shouwen,Guan Yudong,Schlüter Hartmut
The structural instability and electronegativity of sialic acids make N-sialoglycan profiling challenging. We compared commonly used preparation strategies, including native, acetohydrazide-based derivatization, and permethylation, in N-glycomics with nMatch, an R-based bioinformatics tool that ensures full coverage of the detected N-glycans using mass spectrometry. We demonstrated that permethylation leads to efficient detection of not only N-sialoglycans but also other N-glycans and, therefore, provides a valuable reference for the selection of the appropriate N-glycan preparation strategy in future studies.
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DOI
PubMed
高影响因子
Journal of the American Chemical Society
·
2024
·
IF 14.4
Weihang Li,Haoyang Jiang,Xiang Zhang,Bo Lei,Le Li,Haoshen Zhou,Miao Zhong
Electrified synthesis of high-value organonitrogen chemicals from low-cost carbon- and nitrogen-based feedstocks offers an economically and environmentally appealing alternative to traditional thermocatalytic methods. However, the intricate electrochemical reactions at electrode surfaces pose significant challenges in controlling selectivity and activity, especially for producing complex substances such as N,N-dimethylformamide (DMF). Herein, we tackle this challenge by developing relay catalysis for efficient DMF production using a composite WO2–NiOOH/Ni catalyst with two distinctive active sites. Specifically, WO2 selectively promotes dimethylamine (DMA) electrooxidation to produce strongly surface-bound (CH3)2N*, while nearby NiOOH facilitates methanol electrooxidation to yield more weakly bound *CHO. The disparity in binding energetics of the key C- and N-intermediates expedites C–N coupling at the WO2–NiOOH interface. In situ infrared spectroscopy with isotope-labeling experiments, quasi-in situ electron paramagnetic resonance trapping experiments, and electrochemical operating experiments revealed the C–N coupling mechanism and enhanced DMF-synthesis selectivity and activity. In situ X-ray absorption spectroscopy (XAS) and postreaction transmission electron microscopy (TEM) studies verified the stability of WO2–NiOOH/Ni during extended electrochemical operation. A Faradaic efficiency of ∼50% and a production rate of 438 μmol cm–2 h–1 were achieved at an industrially relevant current density of 100 mA cm–2 over an 80 h DMF production period. This study introduces a new paradigm for developing electrothermo relay catalysis for the sustainable and efficient synthesis of valuable organic chemicals with industrial potential.
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Qing Gao,Hailin Fu,Hui Liu,Pengfei Li,Jin Shi,Yao Lin,Ziyuan Song
Poly(sarcosine) (PSar)-based diblock copolypeptoid nanoparticles are promising alternatives to PEGylated polymeric nanocarriers. However, their preparation was limited by the slow polymerization kinetics and low assembly concentrations. Herein, we report the facile preparation of copolypeptoid nanoparticles through polymerization-induced self-assembly of N -substituted glycine N -carboxyanhydrides (NNCAs) in solvents with low polarity and weak hydrogen-bonding ability like dichloromethane (DCM). In contrast to the conventional polymerization in N , N -dimethylformamide (DMF), NNCAs were polymerized at an accelerated rate in DCM that minimized side reactions from water and other impurities. With isopentylamine-initiated PSar as the first stabilizing block, the polymerization of N -benzyl glycine N -carboxyanhydrides resulted in the formation of spherical or rod-like nano-assemblies at high solid contents (up to 15%) within several hours. We believe this work not only improves our basic understanding on NNCA polymerization behaviours, but also offers an efficient strategy to prepare drug nanocarriers with PSar corona, facilitating the downstream application studies.
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Journal of Oncology
·
2022
·
IF 4.501
Sheng-Xiong Zhang,Wen Tian,Yuan-Liang Liu,Jia-Hui Ni,Dan Zhang,Hua-Feng Pan,Zi-Ming Zhao,Bo Ai,Zhe-Sheng Chen,Li-Zhu Lin,Wei Liu
Early diagnosis and treatment of gastric precancerous lesions (GPL) are key factors for reducing the incidence and morbidity of gastric cancer. The study is aimed at examining GPL in mice induced by N-methyl-N-nitroso-urea (MNU) and to illustrate the underlying mechanisms of tumorigenesis. In this study, we utilized an in vivo MNU-induced GPL mouse model, and histopathological changes of the gastric mucosa were observed by hematoxylin and eosin (H&E-stain) and alcian blue (AB-PAS-stain). The level of miR-194-5p in the gastric mucosa was determined by real-time polymerase chain reaction. We used transmission electron microscopy to observe the effects of MNU on gastric chief cells and parietal cells. We performed immunohistochemical detection of HIF-1α, vWF, Ki-67, and P53, while the changes in the protein expression of key genes in LKB1-AMPK and AKT-FoxO3 signaling pathways were detected by western blot analysis. We demonstrated that the miR-194-5p expression was upregulated under hypoxia in GPL gastric tissues, and that a high miR-194-5p expression level closely related with tumorigenesis. Mechanistically, miR-194-5p exerted the acceleration of activities related to metabolic reprogramming through LKB1-AMPK and AKT-FoxO3 pathways. Furthermore, similar to miR-194-5p, high expression levels of AMPK and AKT were also related to the metabolic reprogramming of GPL. Moreover, we revealed the correlation between the expression levels of miR-194-5p, p-AMPKα, p-AKT, and FoxO3a. These findings suggest that miR-194-5p/FoxO3 pathway is important for the reversal of metabolic reprogramming in GPL. Thus, exploring strategies to regulate the miR-194-5p/FoxO3a pathway may provide an efficient strategy for the prevention and treatment of GPL.
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PubMed
高影响因子
Journal of Biobased Materials and Bioenergy
·
2016
·Vol. 10
·期 5
·378-384
·
IF 0.635
Chen Wei,Jiang Yetao,Sun Yong,Wang Yanjun,Li Zheng,Wang Xiaoyu,Zeng Xianhai,Liu Shijie,Lin Lu
5-Dimethylaminomethyl-2-Furanmethanol (DMMF) is an essential precursor for the production of ranitidine, a drug for gastropathy. Herein a facile pathway to the value-added pharmaceutical intermediate DMMF from carbohydrates, such as glucose and fructose, was developed for the first
time. The reaction was carried out under catalyst-free conditions using N, N-dimethyl formamide (DMF) as the amine donor in formic acid (FA). A plausible reaction mechanism from carbohydrates to DMMF was also proposed.
研究领域:
有机化学
·
药物化学
·
生物转化
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高影响因子
FLUID PHASE EQUILIBRIA
·
2015
·
IF 2.2
Xiaofei Yan,Danxing Zheng,Min Zhang,Wenzhi Yang
To develop novel working pairs for absorption power cycles, this study selected 1,1,1,3,3,3-hexafluoropropane (HFC236fa) + N , N -dimethylformamide (DMF) and HFC236fa + N -methyl-2-pyrrolidone (NMP) systems. In addition, the vapor–liquid equilibrium (VLE) data of the two systems were measured in a temperature range extending from 293.15 K to 353.15 K and a pressure range of 30–900 kPa. The five-parameter non-random two liquid (NRTL) model was chosen to regress the obtained VLE data. The results indicated that the NRTL model was suitable for the regression of the VLE data, and the maximum deviations of the pressure between the experimental and calculated values were 4.06% and 3.83%, and the average relative deviations were 1.55% and 1.90%, respectively. The regressed activity coefficients were both less than 1, which indicated that the two binary mixtures exhibit negative deviations from Raoult’s law. Therefore, the two systems exhibit good affinity. However, the activity coefficient of HFC236fa + DMF is smaller than that of HFC236fa + NMP. Finally, the systems in the literature were compared to this work, and the affinity of the systems was analyzed based on the molecular structure and hydrogen bond .
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高影响因子
THERMOCHIMICA ACTA
·
2021
·
IF 3.115
Fenhong Song,Qingli Wang,Qinglong Meng,Han Mu,Jing Fan
Recently, biodiesel has received tremendous attention from a lot of scientists as a biodegradable, renewable and high-potential fuel which could be used directly or blended with diesel in internal combustion engines. The adequate thermophysics properties of fuel are required to model the process of fuel spray, atomization and combustion accurately. In this work, three fuel blends containing methyl laurate and n-undecane, n-dodecane, n-tridecane were prepared and an important transport property—liquid thermal conductivity of which were measured by the transient hot wire method system. The measurement was conducted at temperature ranging from 292 K to 362 K at atmospheric pressure with the mass fraction of methyl laurate being nearly 0.2000, 0.4000, 0.6000 and 0.8000 (by weight) in the binary mixtures. Furthermore, the relationship between the experimental results and temperature and concentration of methyl laurate were correlated as a function. The uncertainty of the experimental system is less than ±2.0%.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2018
·
IF 2.196
Jian Yang,Xianyang Meng,Jiangtao Wu
As the important industrial raw materials, n-pentene, n-hexene, and n-heptene are widely used as gasoline additives to improve the combustion characteristics of gasoline and polymerized monomer to synthesize many important polymer compounds. Therefore, reliable density data are significant for these fluids to apply in industry. In this work, new experimental densities of n-pentene, n-hexene, and n-heptene have been measured at pressures up to 100 MPa along nine isotherms between 283 and 363 K by using a high pressure vibrating-tube densimeter. The experimental system was calibrated using water and a vacuum and was tested with R134a over the entire temperature and pressure ranges. The combined expanded uncertainties of the temperature, pressure, and density with a level of confidence of 0.95 (k = 2) are estimated to be 16 mK, 0.062 MPa (p ≤ 60 MPa), 0.192 MPa (60 MPa < p < 100 MPa), and up to 0.6 kg·m–3 depending on the temperature and pressure ranges. The density data were correlated with the Tait-like equation, and the average absolute deviations are 0.06, 0.04, and 0.05%. Furthermore, the isothermal compressibility and the isobaric thermal expansivity were derived from the Tait-like equation over the experimental temperature and pressure ranges.
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高影响因子
PROCEEDINGS OF THE COMBUSTION INSTITUTE
·
2024
·
IF 5.3
Qilong Fang,Jun Fang,Yi Zhang,Jianguo Zhang,Tianyou Lian,Wei Li,Yuyang Li
Oxymethylene ethers (OMEs) have received great attention due to their carbon-neutral nature and advantageous properties as alternative transportation fuels or fuel additives. Previous studies focused on the combustion of linear OMEs with the chemical formula of CH 3 O(CH 2 O) m CH 3 ( m ≥ 0), while a deep understanding of the combustion of branched OMEs with the chemical formula of CH 3 OCH 3- n (OCH 3 ) n (0 ≤ n ≤ 3) is still absent. In this work, the flow reactor pyrolysis of dimethoxymethane (DMM, n = 1), trimethoxymethane (TMM, n = 2), and tetramethoxymethane (TeMM, n = 3) is investigated using gas chromatography, with special attention given to the multi-sidechain effects on the pyrolysis chemistry. A pyrolysis model of branched OMEs is developed and validated against the new pyrolysis data. Modeling analysis is performed to provide insights into the pyrolysis chemistry of the three branched OMEs and the multi-sidechain effects. Unimolecular decomposition and H-abstraction reactions are found to be two major types of fuel decomposition pathways, with the former dominating TMM decomposition, the latter dominating DMM decomposition, and both contributing almost equally to TeMM decomposition. Sensitivity analysis shows that C O bond dissociation is the most sensitive reaction to fuel decomposition in DMM pyrolysis, while methanol elimination is the most sensitive reaction in TMM and TeMM pyrolysis. Among the three fuels, DMM has the lowest pyrolysis reactivity and produces the least abundant methanol. In contrast, TMM is observed with the highest pyrolysis reactivity and produces the most abundant methanol. Theoretical calculations reveal that TMM and DMM have the lowest and highest energy barriers of methanol elimination reactions, which determines the order of their pyrolysis reactivities and methanol formation trends. As a result, DMM pyrolysis occurs at higher temperature regions where the C O bond dissociation and H-abstraction reactions are preferred, while TMM pyrolysis occurs at lower temperature region where methanol elimination dominates.
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2016
·
IF 1.78
Xue-Jie Tan,Shuang-Shuang Cheng,Yan Shi,Dian-Xiang Xing,Yun Liu,Hui Li,Wen-Quan Feng,Jian-Bo Yang
Maleimide groups are used extensively in bioconjugation reactions, but limited mechanistic studies are available regarding their hydrolysis reactions . In this paper, five single-crystal structures related with the reaction of four-step hydrolytic degradation of N,N ′-ethylenedimaleimide have been investigated. On the basis of experimental results, the reaction mechanisms without or with water catalysis are proposed, which could provide some enlightenment into the study of similar hydrolytic degradations.
研究领域:
化学
·
生物学
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高影响因子
JOURNAL OF PHYSICAL CHEMISTRY A
·
2017
·
IF 2.847
Anne Rodriguez,Olivier Herbinet,Xiangzan Meng,Christa Fittschen,Zhandong Wang,Lili Xing,Lidong Zhang,Frédérique Battin-Leclerc
A wide range of hydroperoxides (C1–C3 alkyl hydroperoxides, C3–C7 alkenyl hydroperoxides, C7 ketohydroperoxides, and hydrogen peroxide (H2O2)), as well as ketene and diones, have been quantified during the gas-phase oxidation of n-heptane. Some of these species, as well as C10 alkenyl hydroperoxides and ketohydroperoxides, were also measured during the oxidation of n-decane. These experiments were performed using an atmospheric-pressure jet-stirred reactor at temperatures from 500 to 1100 K and one of three analytical methods, time-of-flight mass spectrometry combined with tunable synchrotron photoionization with a molecular beam sampling: time-of-flight mass spectrometry combined with laser photoionization with a capillary tube sampling, continuous wave cavity ring-down spectroscopy with sonic probe sampling. The experimental temperature at which the maximum mole fraction is observed increases significantly for alkyl hydroperoxides, alkenyl hydroperoxides, and then more so again for hydrogen peroxide, compared to ketohydroperoxides. The influence of the equivalence ratio from 0.25 to 4 on the formation of these peroxides has been studied during n-heptane oxidation. The up-to-date detailed kinetic oxidation models for n-heptane and for n-decane found in the literature have been used to discuss the possible pathways by which these peroxides, ketene, and diones are formed. In general, the model predicts well the reactivity of the two fuels, as well as the formation of major intermediates.
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高影响因子
JOURNAL OF POWER SOURCES
·
2018
·
IF 6.945
Bo Tong,Jiawei Wang,Zhenjie Liu,Lipo Ma,Peng Wang,Wenfang Feng,Zhangquan Peng,Zhibin Zhou
Formation of dendrite on lithium metal anode (LMA) poses a threat to commercial prospects of rechargeable lithium metal batteries (LMBs). Electrolyte engineering, including but not limited to using new solvents, salts and additives, is an effective remedy to surmounting this vexing problem. In this work, a novel additive, trimethylsilyl(fluorosulfonyl)( n -nonafluorobutanesulfonyl)imide {(CH 3 ) 3 Si-N[(FSO 2 )( n -C 4 F 9 SO 2 )], TMS-FNFSI}, is introduced to the ether-based electrolyte of LiN[(CF 3 SO 2 ) 2 ]-1,3-dioxolane/dimethoxyethane (LiTFSI-DOL/DME) for LMBs. It preferentially reduces on LMA prior to the main electrolyte components (ethers and LiTFSI), resulting in a LiF-rich, organic F- and Si-containing solid electrolyte interphase (SEI) film, being validated by X-ray photoelectron spectroscopy (XPS). Thus, dendrite growth is effectively suppressed. Li|Cu cells, with 1.0 M LiTFSI-DOL/DME (1:1, v/v) containing 5 wt% TMS-FNFSI, exhibit an average coulombic efficiency up to 96.5% for ca. 100 cycles at the current density and areal capacity of 1 mA cm −2 and 2 mAh cm −2 , respectively. The Li|Li symmetric cells with TMS-FNFSI can run for more than 1200 h with a stable polarization voltage (ca. 25 mV) at 0.5 mA cm −2 . The Li|LiFePO 4 cells with TMS-FNFSI can operate steadily for 100 cycles with capacity retention of 92% at 0.2 C. The reduction decomposition mechanism of TMS-FNFSI is tentatively proposed.
研究领域:
电化学
·
能源材料
·
电池技术
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高影响因子
危险品化学品经营许可证（带存储）